LOCUS       DAPBBG010000001       112685 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPBBG010000001 DAPBBG010000000
VERSION     DAPBBG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 112685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 112685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..112685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.001"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            310..1449
                     /locus_tag="RD477_000001"
     CDS             310..1449
                     /locus_tag="RD477_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDT7632404.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1516..2091)
                     /locus_tag="RD477_000002"
     CDS             complement(1516..2091)
                     /locus_tag="RD477_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7632405.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2096..3070)
                     /locus_tag="RD477_000003"
     CDS             complement(2096..3070)
                     /locus_tag="RD477_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDT7632406.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3094..3138)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3353..4321
                     /locus_tag="RD477_000004"
     CDS             3353..4321
                     /locus_tag="RD477_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7632407.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            4418..4711
                     /locus_tag="RD477_000005"
     CDS             4418..4711
                     /locus_tag="RD477_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDT7632408.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4829..5065
                     /gene="secG"
                     /locus_tag="RD477_000006"
     CDS             4829..5065
                     /gene="secG"
                     /locus_tag="RD477_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDT7632409.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5168..5923
                     /locus_tag="RD477_000007"
     CDS             5168..5923
                     /locus_tag="RD477_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7632410.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5926..8283
                     /gene="rnr"
                     /locus_tag="RD477_000008"
     CDS             5926..8283
                     /gene="rnr"
                     /locus_tag="RD477_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDT7632411.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8305..8769
                     /gene="smpB"
                     /locus_tag="RD477_000009"
     CDS             8305..8769
                     /gene="smpB"
                     /locus_tag="RD477_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDT7632412.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9071..9790
                     /gene="atpB"
                     /locus_tag="RD477_000010"
     CDS             9071..9790
                     /gene="atpB"
                     /locus_tag="RD477_000010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDT7632413.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9843..10058
                     /gene="atpE"
                     /locus_tag="RD477_000011"
     CDS             9843..10058
                     /gene="atpE"
                     /locus_tag="RD477_000011"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDT7632414.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10149..10673
                     /gene="atpF"
                     /locus_tag="RD477_000012"
     CDS             10149..10673
                     /gene="atpF"
                     /locus_tag="RD477_000012"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDT7632415.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10660..11202
                     /locus_tag="RD477_000013"
     CDS             10660..11202
                     /locus_tag="RD477_000013"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDT7632416.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11225..12781
                     /locus_tag="RD477_000014"
     CDS             11225..12781
                     /locus_tag="RD477_000014"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDT7632417.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12795..13697
                     /locus_tag="RD477_000015"
     CDS             12795..13697
                     /locus_tag="RD477_000015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDT7632418.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEVSSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13720..15126
                     /gene="atpD"
                     /locus_tag="RD477_000016"
     CDS             13720..15126
                     /gene="atpD"
                     /locus_tag="RD477_000016"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDT7632419.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15140..15562
                     /locus_tag="RD477_000017"
     CDS             15140..15562
                     /locus_tag="RD477_000017"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDT7632420.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16031..16261
                     /locus_tag="RD477_000018"
     CDS             16031..16261
                     /locus_tag="RD477_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDT7632421.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16358..17659
                     /gene="murA"
                     /locus_tag="RD477_000019"
     CDS             16358..17659
                     /gene="murA"
                     /locus_tag="RD477_000019"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDT7632422.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17672..17848
                     /locus_tag="RD477_000020"
     CDS             17672..17848
                     /locus_tag="RD477_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDT7632423.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17983..18279
                     /gene="yidD"
                     /locus_tag="RD477_000021"
     CDS             17983..18279
                     /gene="yidD"
                     /locus_tag="RD477_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDT7632424.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18585..19052
                     /locus_tag="RD477_000022"
     CDS             18585..19052
                     /locus_tag="RD477_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7632425.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            19137..20021
                     /locus_tag="RD477_000023"
     CDS             19137..20021
                     /locus_tag="RD477_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7632426.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            20135..20371
                     /locus_tag="RD477_000024"
     CDS             20135..20371
                     /locus_tag="RD477_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDT7632427.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20459..20539
                     /locus_tag="RD477_000025"
     tRNA            20459..20539
                     /locus_tag="RD477_000025"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20493..20495,aa:Tyr,seq:gta)
     gene            20546..20617
                     /locus_tag="RD477_000026"
     tRNA            20546..20617
                     /locus_tag="RD477_000026"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20578..20580,aa:Gln,seq:ttg)
     gene            20742..20912
                     /locus_tag="RD477_000027"
     CDS             20742..20912
                     /locus_tag="RD477_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632428.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            21132..21839
                     /locus_tag="RD477_000028"
     CDS             21132..21839
                     /locus_tag="RD477_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7632429.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21898..22542)
                     /locus_tag="RD477_000029"
     CDS             complement(21898..22542)
                     /locus_tag="RD477_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDT7632430.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            22668..22955
                     /locus_tag="RD477_000030"
     CDS             22668..22955
                     /locus_tag="RD477_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632431.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            23063..23383
                     /locus_tag="RD477_000031"
     CDS             23063..23383
                     /locus_tag="RD477_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7632432.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23464..24777
                     /locus_tag="RD477_000032"
     CDS             23464..24777
                     /locus_tag="RD477_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDT7632433.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            24774..25493
                     /locus_tag="RD477_000033"
     CDS             24774..25493
                     /locus_tag="RD477_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDT7632434.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            25621..26172
                     /gene="raiA"
                     /locus_tag="RD477_000034"
     CDS             25621..26172
                     /gene="raiA"
                     /locus_tag="RD477_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDT7632435.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26394..28928
                     /gene="secA"
                     /locus_tag="RD477_000035"
     CDS             26394..28928
                     /gene="secA"
                     /locus_tag="RD477_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDT7632436.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            29054..30155
                     /gene="prfB"
                     /locus_tag="RD477_000036"
     CDS             join(29054..29125,29127..30155)
                     /gene="prfB"
                     /locus_tag="RD477_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDT7632437.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            30260..30946
                     /gene="ftsE"
                     /locus_tag="RD477_000037"
     CDS             30260..30946
                     /gene="ftsE"
                     /locus_tag="RD477_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDT7632438.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30930..31823
                     /locus_tag="RD477_000038"
     CDS             30930..31823
                     /locus_tag="RD477_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632439.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31975..33423
                     /gene="cls"
                     /locus_tag="RD477_000039"
     CDS             31975..33423
                     /gene="cls"
                     /locus_tag="RD477_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDT7632440.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            33760..34617
                     /locus_tag="RD477_000040"
     CDS             33760..34617
                     /locus_tag="RD477_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDT7632441.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            34732..35652
                     /gene="pstC"
                     /locus_tag="RD477_000041"
     CDS             34732..35652
                     /gene="pstC"
                     /locus_tag="RD477_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDT7632442.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            35652..36536
                     /gene="pstA"
                     /locus_tag="RD477_000042"
     CDS             35652..36536
                     /gene="pstA"
                     /locus_tag="RD477_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDT7632443.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            36551..37357
                     /gene="pstB"
                     /locus_tag="RD477_000043"
     CDS             36551..37357
                     /gene="pstB"
                     /locus_tag="RD477_000043"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDT7632444.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            37373..38131
                     /locus_tag="RD477_000044"
     CDS             37373..38131
                     /locus_tag="RD477_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDT7632445.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            38144..38821
                     /gene="phoU"
                     /locus_tag="RD477_000045"
     CDS             38144..38821
                     /gene="phoU"
                     /locus_tag="RD477_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDT7632446.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            39058..40089
                     /locus_tag="RD477_000046"
     CDS             39058..40089
                     /locus_tag="RD477_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDT7632447.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            40086..40853
                     /locus_tag="RD477_000047"
     CDS             40086..40853
                     /locus_tag="RD477_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDT7632448.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            40953..42521
                     /gene="liaX"
                     /locus_tag="RD477_000048"
     CDS             40953..42521
                     /gene="liaX"
                     /locus_tag="RD477_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDT7632449.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            42818..43138
                     /locus_tag="RD477_000049"
     CDS             42818..43138
                     /locus_tag="RD477_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDT7632450.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            43138..43488
                     /locus_tag="RD477_000050"
     CDS             43138..43488
                     /locus_tag="RD477_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDT7632451.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            43655..44593
                     /locus_tag="RD477_000051"
     CDS             43655..44593
                     /locus_tag="RD477_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDT7632452.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            44608..45438
                     /locus_tag="RD477_000052"
     CDS             44608..45438
                     /locus_tag="RD477_000052"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDT7632453.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            45482..46507
                     /locus_tag="RD477_000053"
     CDS             45482..46507
                     /locus_tag="RD477_000053"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDT7632454.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            46522..47460
                     /gene="galU"
                     /locus_tag="RD477_000054"
     CDS             46522..47460
                     /gene="galU"
                     /locus_tag="RD477_000054"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDT7632455.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            47585..48025
                     /locus_tag="RD477_000055"
     CDS             47585..48025
                     /locus_tag="RD477_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDT7632456.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            48027..48629
                     /locus_tag="RD477_000056"
     CDS             48027..48629
                     /locus_tag="RD477_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7632457.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(48688..49794)
                     /locus_tag="RD477_000057"
     CDS             complement(48688..49794)
                     /locus_tag="RD477_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDT7632458.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKILP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            50004..51005
                     /gene="ccpA"
                     /locus_tag="RD477_000058"
     CDS             50004..51005
                     /gene="ccpA"
                     /locus_tag="RD477_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDT7632459.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(51342..53687)
                     /locus_tag="RD477_000059"
     CDS             complement(51342..53687)
                     /locus_tag="RD477_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDT7632460.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            53966..54868
                     /locus_tag="RD477_000060"
     CDS             53966..54868
                     /locus_tag="RD477_000060"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDT7632461.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            55081..56856
                     /locus_tag="RD477_000061"
     CDS             55081..56856
                     /locus_tag="RD477_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDT7632462.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(56922..58508)
                     /locus_tag="RD477_000062"
     CDS             complement(56922..58508)
                     /locus_tag="RD477_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDT7632463.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            58759..58983
                     /locus_tag="RD477_000063"
     CDS             58759..58983
                     /locus_tag="RD477_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDT7632464.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            59125..60876
                     /locus_tag="RD477_000064"
     CDS             59125..60876
                     /locus_tag="RD477_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7632465.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            60876..62660
                     /locus_tag="RD477_000065"
     CDS             60876..62660
                     /locus_tag="RD477_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632466.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVECGNHETLLAQNGLYADMYKLQSSGV"
     gene            63118..63969
                     /locus_tag="RD477_000066"
     CDS             63118..63969
                     /locus_tag="RD477_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7632467.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQISTDEKMYLTIHTARL
                     VHENRCK"
     gene            64066..65913
                     /locus_tag="RD477_000067"
                     /pseudo
     CDS             64066..65913
                     /locus_tag="RD477_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
     gene            complement(65954..66856)
                     /locus_tag="RD477_000068"
     CDS             complement(65954..66856)
                     /locus_tag="RD477_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7632468.1"
                     /translation="MLLREKIKQISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLDETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            67086..68489
                     /locus_tag="RD477_000069"
     CDS             67086..68489
                     /locus_tag="RD477_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDT7632469.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            68486..69922
                     /locus_tag="RD477_000070"
     CDS             68486..69922
                     /locus_tag="RD477_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDT7632470.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDEDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            70067..70993
                     /gene="trxB"
                     /locus_tag="RD477_000071"
     CDS             70067..70993
                     /gene="trxB"
                     /locus_tag="RD477_000071"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDT7632471.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            71207..72934
                     /locus_tag="RD477_000072"
     CDS             71207..72934
                     /locus_tag="RD477_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDT7632472.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EENHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            72949..73299
                     /locus_tag="RD477_000073"
     CDS             72949..73299
                     /locus_tag="RD477_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDT7632473.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(73391..74251)
                     /locus_tag="RD477_000074"
     CDS             complement(73391..74251)
                     /locus_tag="RD477_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDT7632474.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            74343..74771
                     /locus_tag="RD477_000075"
     CDS             74343..74771
                     /locus_tag="RD477_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7632475.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            74933..75109
                     /locus_tag="RD477_000076"
     CDS             74933..75109
                     /locus_tag="RD477_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDT7632476.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            75136..75582
                     /locus_tag="RD477_000077"
     CDS             75136..75582
                     /locus_tag="RD477_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDT7632477.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(75736..77115)
                     /gene="brnQ"
                     /locus_tag="RD477_000078"
     CDS             complement(75736..77115)
                     /gene="brnQ"
                     /locus_tag="RD477_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDT7632478.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            77821..78792
                     /locus_tag="RD477_000079"
     CDS             77821..78792
                     /locus_tag="RD477_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDT7632479.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            78816..81008
                     /locus_tag="RD477_000080"
     CDS             78816..81008
                     /locus_tag="RD477_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDT7632480.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            81025..81501
                     /gene="ybeY"
                     /locus_tag="RD477_000081"
     CDS             81025..81501
                     /gene="ybeY"
                     /locus_tag="RD477_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDT7632481.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            81485..81880
                     /locus_tag="RD477_000082"
     CDS             81485..81880
                     /locus_tag="RD477_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDT7632482.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIDWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            81895..82794
                     /gene="era"
                     /locus_tag="RD477_000083"
     CDS             81895..82794
                     /gene="era"
                     /locus_tag="RD477_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDT7632483.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            82937..83749
                     /gene="recO"
                     /locus_tag="RD477_000084"
     CDS             82937..83749
                     /gene="recO"
                     /locus_tag="RD477_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDT7632484.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            84133..85050
                     /gene="glyQ"
                     /locus_tag="RD477_000085"
     CDS             84133..85050
                     /gene="glyQ"
                     /locus_tag="RD477_000085"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDT7632485.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            85052..87127
                     /locus_tag="RD477_000086"
     CDS             85052..87127
                     /locus_tag="RD477_000086"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDT7632486.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            87511..88290
                     /locus_tag="RD477_000087"
     CDS             87511..88290
                     /locus_tag="RD477_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632487.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            88331..89275
                     /locus_tag="RD477_000088"
     CDS             88331..89275
                     /locus_tag="RD477_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7632488.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            89291..90070
                     /locus_tag="RD477_000089"
     CDS             89291..90070
                     /locus_tag="RD477_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDT7632489.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            90083..90781
                     /locus_tag="RD477_000090"
     CDS             90083..90781
                     /locus_tag="RD477_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDT7632490.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            90809..91573
                     /locus_tag="RD477_000091"
     CDS             90809..91573
                     /locus_tag="RD477_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDT7632491.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            91618..92307
                     /locus_tag="RD477_000092"
                     /pseudo
     CDS             91618..92307
                     /locus_tag="RD477_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            92660..92823
                     /locus_tag="RD477_000093"
                     /pseudo
     CDS             92660..92823
                     /locus_tag="RD477_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            94169..95419
                     /locus_tag="RD477_000094"
     CDS             94169..95419
                     /locus_tag="RD477_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDT7632492.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            95438..96448
                     /locus_tag="RD477_000095"
     CDS             95438..96448
                     /locus_tag="RD477_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDT7632493.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            96458..97111
                     /locus_tag="RD477_000096"
     CDS             96458..97111
                     /locus_tag="RD477_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7632494.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            97130..98128
                     /locus_tag="RD477_000097"
     CDS             97130..98128
                     /locus_tag="RD477_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDT7632495.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            98204..98965
                     /locus_tag="RD477_000098"
     CDS             98204..98965
                     /locus_tag="RD477_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDT7632496.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            98983..100089
                     /locus_tag="RD477_000099"
     CDS             98983..100089
                     /locus_tag="RD477_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7632497.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            100511..100966
                     /locus_tag="RD477_000100"
     CDS             100511..100966
                     /locus_tag="RD477_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HDT7632498.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            100980..101753
                     /locus_tag="RD477_000101"
     CDS             100980..101753
                     /locus_tag="RD477_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7632499.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            101740..102870
                     /locus_tag="RD477_000102"
     CDS             101740..102870
                     /locus_tag="RD477_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7632500.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            102901..104136
                     /locus_tag="RD477_000103"
     CDS             102901..104136
                     /locus_tag="RD477_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632501.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            104161..105270
                     /locus_tag="RD477_000104"
     CDS             104161..105270
                     /locus_tag="RD477_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632502.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            105312..106646
                     /gene="gmhB"
                     /locus_tag="RD477_000105"
     CDS             105312..106646
                     /gene="gmhB"
                     /locus_tag="RD477_000105"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDT7632503.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            106681..108318
                     /locus_tag="RD477_000106"
     CDS             106681..108318
                     /locus_tag="RD477_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDT7632504.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            108420..108815
                     /locus_tag="RD477_000107"
     CDS             108420..108815
                     /locus_tag="RD477_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDT7632505.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            108833..109564
                     /locus_tag="RD477_000108"
     CDS             108833..109564
                     /locus_tag="RD477_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7632506.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            109588..111039
                     /locus_tag="RD477_000109"
     CDS             109588..111039
                     /locus_tag="RD477_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDT7632507.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            111065..111676
                     /locus_tag="RD477_000110"
     CDS             111065..111676
                     /locus_tag="RD477_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7632508.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            111858..112373
                     /locus_tag="RD477_000111"
     CDS             111858..112373
                     /locus_tag="RD477_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HDT7632509.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(112508..>112685)
                     /locus_tag="RD477_000112"
     CDS             complement(112508..>112685)
                     /locus_tag="RD477_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7632510.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
BASE COUNT        37032 a        19051 c        24141 g        32461 t
ORIGIN      
        1 atatcagcag gtagagagtt tttttgcaac tagcttctgt atatgatgtt tacaaagaat
       61 ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga
      121 gcttttaagg atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa
      181 ataggaggga agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt
      241 ggttgctcct atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga
      301 gaggattaga tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa
      361 tcagtgaatg gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaaacat
      421 gacataacag gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac
      481 caaaagggcc aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct
      541 ggtgatgaat cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg
      601 tacggacgtg gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc
      661 gaactgaaag agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga
      721 gaagaagtgg gtgaactagg tggggaacag ctcacgaaag caggctatgt agatgactta
      781 gatgccttga tcatcggaga gccaaccaat tacagtttga tgtatacgca catgggatca
      841 atcaactata cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt
      901 tataatgcaa tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac
      961 ctcgctgaaa caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc
     1021 agtggcggga atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga
     1081 tcgattccag aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa
     1141 ttgaatcaag agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc
     1201 aaagctgatc cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta
     1261 cctttagttg gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc
     1321 ttcgactttg tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat
     1381 gttgaaatcg ataattattt ggatatgatc gaaaaatatc aaggaattat tttatcctac
     1441 ttagcgtaag atttttttaa taaatgaaaa aacagagcca gattagcatt attttgctaa
     1501 tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt gtggcaacat
     1561 agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt ctgtacctgc
     1621 ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata gctgaacgat
     1681 catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga tcgaagaaac
     1741 gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc tgtttttatc
     1801 tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa tttggattgg
     1861 cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc tggacaatcc
     1921 tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc
     1981 gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat tgatcccaat
     2041 acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca ttttgttagc
     2101 ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg
     2161 ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta ctgttagcag
     2221 gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt ccttgagttt
     2281 ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa taagcaacag
     2341 ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg aagtcaaaat
     2401 aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga cagcagtatt
     2461 cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc atttctatcg
     2521 tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag taattgtatt
     2581 cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag acaaaacgtt
     2641 ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg gtcaaaggac
     2701 ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa aaatcaacgg
     2761 catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc cctaaaccat
     2821 ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt tccattcctt
     2881 gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca ttctgcgtac
     2941 ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg atatcagggg
     3001 atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta
     3061 cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta ttctgggaag
     3121 tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag tatactggct
     3181 tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac tttaagtaaa
     3241 tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc ctatggatcg
     3301 gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta caatggttat
     3361 tttttatttc attcttattc tgataattat agggatcagt taccattttc tttcttattt
     3421 ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt atgaagacac
     3481 tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac gacaaaaatt
     3541 agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca aaagttatga
     3601 cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata aatgggtcat
     3661 ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag gtaaaagata
     3721 tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag aaagtgaagg
     3781 agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga tccaactcat
     3841 cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg gagcttctac
     3901 gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta ttttagacag
     3961 cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca ctgtctttcc
     4021 taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg gttactcctt
     4081 aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat tagtcatcca
     4141 tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa atgctacggc
     4201 aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat cgatgaaaga
     4261 tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa cgatgaatta
     4321 attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag atcaggagac
     4381 tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg catatggaaa
     4441 tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga tcggagaaat
     4501 gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg tagatccagc
     4561 atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat tagcaagacg
     4621 agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg aaaagaaacc
     4681 agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt tgaactttca
     4741 gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa gttaaagaga
     4801 cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa tcgtgatcgt
     4861 tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa atagtgcagc
     4921 gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc gcggctttga
     4981 agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct tattatttgt
     5041 cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc gggcggcttt
     5101 ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt aaaatgaggc
     5161 gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac atggaaagcg
     5221 agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa tgctcgcacg
     5281 atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac acggaacaat
     5341 ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca ctaaaaaagc
     5401 catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat tatcaatggg
     5461 cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg gttttttctg
     5521 ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat acgtacgtca
     5581 agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag ttgcttaccg
     5641 ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg tagaaagtag
     5701 gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg aaatgatcga
     5761 ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga cacttcaatg
     5821 gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat tagaaaaaga
     5881 cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac aaaagaaacg
     5941 ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt ttctatggaa
     6001 gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact agtacaaacg
     6061 attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa agtgaaactt
     6121 cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg atttggattt
     6181 gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac tggttttgca
     6241 atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga tatggatcgc
     6301 ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt agcaggggaa
     6361 ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt tgttatcccg
     6421 aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt tcgtccagtt
     6481 gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta tgcgactagt
     6541 ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat ggatatcctc
     6601 tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt gtctgaagca
     6661 gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga tttaagaaat
     6721 cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc agttacggtt
     6781 aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt ttcttactat
     6841 gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag tgtatatttg
     6901 actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg ttcactgaat
     6961 ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa tggacaagta
     7021 gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac ctacacagct
     7081 gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc tcagctcgta
     7141 cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg aatcaaacgt
     7201 ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga aggacaccca
     7261 aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc tttcatgctt
     7321 gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt tatttaccga
     7381 atacatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc ttcagctttg
     7441 ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca gcatgtgatc
     7501 gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct aagaagcatg
     7561 caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc agattactac
     7621 acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg tctgatccgt
     7681 tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga acttcctgaa
     7741 attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg tgaagtagat
     7801 tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga tgggatcatc
     7861 agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga gggattgatc
     7921 catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt agctcttgta
     7981 ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa agtagtcaaa
     8041 gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga ggttacagcg
     8101 ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa acaagcagga
     8161 aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa gcaaacgttc
     8221 ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa aaagaaaaaa
     8281 taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat tgcacaaaac
     8341 aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat ggtcttgcaa
     8401 ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg atttattcgc
     8461 gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga acaaggaaat
     8521 atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa acaaatcatc
     8581 cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa agtctatatt
     8641 cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc ttatgataaa
     8701 cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt aaaaaatttc
     8761 tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta cttaatgata
     8821 aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc ttagatcagg
     8881 agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa gtgtctaatt
     8941 ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt ggttagaaac
     9001 cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg gggaaaagag
     9061 gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt ggtttgatgg
     9121 tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag cttacttttt
     9181 tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat gggtcattga
     9241 ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca atttccattt
     9301 gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt tagtaacaaa
     9361 aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc cgtttgttac
     9421 tttaactttg gcatttataa tgatcttact tactcactta tttggggtga aaaagttagg
     9481 cttcaaagga tatttcgtaa attcgttctt gaaaccatat agttttatgt ttccgatgaa
     9541 actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg ggaatattta
     9601 tgcgggggaa gttttgctaa ccttgattgc aaaaatggta ataagcttag gctggttctc
     9661 attgccgtta gcaattccat tagagatggt ctggatcgca ttctccttgt ttatcggaag
     9721 catccaagca tttgtcttcg ttactttatc tatggtttat atgagccata agattgaagt
     9781 ggaagaataa tattcgtttt aaaaacaaac tactatttat ctaatttagg aggaaaacaa
     9841 ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga gccggttatg
     9901 gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct gaaatgtctg
     9961 gtcaattccg tacaacaatg tttatcgggg tagctctagt cgaagctgtt cctatcttag
    10021 gggtagttgt cgccttgatt ctagtttttg gtgtttaatt gaatcagtat taaggcagaa
    10081 acaatttggt ttgttactgc ttttttacat gaaaactaga agtacttcaa agaaaggaag
    10141 gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg ttaggaaatg
    10201 tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcat tttgcttggg
    10261 gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat ttggattctg
    10321 cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa caattgttag
    10381 cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga gaattaagcc
    10441 gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagt aaagcccaag
    10501 ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgat gttgcagaac
    10561 tatctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa atgcacgaat
    10621 cattaatcaa tcaatatatc gaaggtctag gttcttcaaa tgaaactaga taaatataca
    10681 gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa tcaagcagaa
    10741 gaaatctatc aagatctcgt aagcttacgt caaatctatg aacaagtacc tggattaggt
    10801 aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat ggacaagctt
    10861 gttagtggct atgatggaat cgttaaaaac tttttagaag ttgtttatgc gtataaccga
    10921 atggatgatc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga acatcaaggt
    10981 ttgctgttgg gaagtgtcac aacagcagtt ccactttcag acgaacaacg aatacaatta
    11041 gaaaagaatg ttgcaaaaac aatgaactat caatcagtcg aattaaaaca aatcgttgat
    11101 tcttctatta ttggtggcgc gattgttgaa gctaaccatc gagtaatcga cggaagcatt
    11161 cgcacgcaat tagaaaatat gcgtaatcaa ttaaatagat agtacaaaga ggtaggtgaa
    11221 tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa ttgaaaatta
    11281 tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg acgggatcgc
    11341 tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt caaatggctc
    11401 atacggaatg gcgcaaaact tagaatcaaa tgatgtaggt atcattatcc ttggcgattt
    11461 cgagtccatt cgtgaaggag ataaagtaaa acgaacagga aagatcatgg aagttccagt
    11521 tggagaggcc ttgatcggac gggtagttaa tccgctaggt cagccaatcg acggactagg
    11581 tgaaatcgtt actgacaaag ctcgtccagt agaagcaatg gcaccaggcg ttatgcaacg
    11641 taaatctgtt aacgaaccaa tgcaaacagg tctaaaagca atcgacgctc ttgtaccaat
    11701 cggacgtggc caacgtgaat tagttatcgg tgaccgtaaa acagggaaaa cttctattgc
    11761 gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg tagcaatcgg
    11821 acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg gcgcaatgga
    11881 ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagcg ccgttgcttt atatcgcacc
    11941 atatgctggt actgcaatgg gtgaagagtt catgtacaac ggtaaacatg tcttgatcat
    12001 ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc tacttcgccg
    12061 tccaccaggt cgtgaagctt atccagggga tgttttctac ttacactcac gcttattgga
    12121 acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct taccatttgt
    12181 tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt cgatcactga
    12241 tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag cggttgatgc
    12301 cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga aaaaagttgc
    12361 tgggacttta cgtctagact tagcaagtta ccgtgaatta gaagcattta cacaattcgg
    12421 ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta cagtagaaat
    12481 cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga ttttgtatgc
    12541 tttgacacat ggattcttag atagtatccc tgtagacagt attttagact tcgaacatga
    12601 attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc gtacaacaaa
    12661 agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag acatgttctt
    12721 agctacaaaa ggaaataatt cctctacaga agataaattg aaatccattc aaaatgcata
    12781 gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc gcttcaacga
    12841 aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag ttgacaaaat
    12901 ctgaagtttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa atcgttacgc
    12961 atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg gatatcaatt
    13021 acaacagcat gctgctcagc cgtcctgtta agaaaactgg ttatatcgtc atcactgcag
    13081 atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg tctattttag
    13141 aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga actggtgcag
    13201 acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta tctgatcagc
    13261 caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgtac caaaacgaag
    13321 tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact agtcagtttc
    13381 gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaact actttcgaac
    13441 aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg cctcagtatg
    13501 ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat gctgctggta
    13561 tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag ctgacggttt
    13621 cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt gttgccggag
    13681 cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg caagattgtt
    13741 caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt accagatatc
    13801 aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt tcttgaagca
    13861 gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac agatggttta
    13921 caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt aggtaaagaa
    13981 acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac acctttccca
    14041 gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga attaagtaca
    14101 agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc atatttaaaa
    14161 ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact gatccaagaa
    14221 ctgatccata atatcgccca agaacatggt ggtatttctg tatttactgg tgtaggtgaa
    14281 cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt tatcgaaaaa
    14341 acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg tgttgctttg
    14401 actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt actgttgttt
    14461 atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt gttaggacgg
    14521 atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca attgcaagaa
    14581 cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta tgtgccagcc
    14641 gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc gacaaccaac
    14701 ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt ggcttcttct
    14761 tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc aacagaagtc
    14821 caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt aggtatggat
    14881 gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca attcttcttg
    14941 tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata tgtccctgta
    15001 gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga tcttcctgaa
    15061 gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa aacactaggc
    15121 tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa ctcctgccgg
    15181 tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg gtgagcttgg
    15241 gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag ttcgtgtaaa
    15301 acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa tcatggaagt
    15361 tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg atatcgatgt
    15421 atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag caaaagcaaa
    15481 acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa tcaatcgtat
    15541 caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt aagaacagta
    15601 aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt acgaaaattg
    15661 cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc tggaacaccg
    15721 tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca aatggaaaat
    15781 agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat tgaacaccgt
    15841 ttatttggaa taagggactg cgacaagact tttgtttcag tccctttttt tgcatatttt
    15901 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
    15961 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
    16021 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
    16081 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
    16141 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
    16201 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
    16261 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
    16321 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
    16381 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
    16441 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
    16501 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
    16561 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
    16621 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
    16681 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
    16741 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
    16801 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
    16861 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
    16921 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
    16981 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
    17041 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
    17101 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
    17161 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
    17221 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
    17281 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
    17341 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
    17401 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
    17461 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
    17521 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
    17581 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
    17641 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
    17701 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
    17761 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
    17821 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
    17881 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
    17941 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
    18001 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
    18061 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
    18121 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
    18181 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
    18241 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
    18301 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
    18361 aaaaaaggtt gtgaaaaata cgagtagcac tgagcgataa agaaacaatt tggaaaatag
    18421 cttcttatat tttgcaaaat tgtgagttat tgccgaagtg ttggattttg aacaccgtca
    18481 aaaaaaaggt tgtgaaaaat acggctagca ctgagcgata aagaaaaaga ggaaatttat
    18541 tgtcaaagta acaactccgc aactacttat atgggggaca aaaaatgatc gaagaactaa
    18601 acaagaaaga tttaccatgg tccttgttat tagatgcaga tcctgataaa gaaaaagttc
    18661 aagtatatgt ttccagaggg agtggctgga tatggaaaga aaaagaaaaa acaataggtg
    18721 tactgatcta tgtggcaagg gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa
    18781 cgtatcaagg aaaggggatt ggcggaaaat tacttgagac agcttttcag aagctttcac
    18841 aactcgcttc cagtcaaact cagatcatca tacgaaccgg tagtactagc agtgcggcgt
    18901 tacatctcta tcaaaaaatg ggctatgtgg agattggtcg agtcaaagac tattttatcc
    18961 atcattactc tgagcccatt tttgaaaatg gagatcaatt aagggatcaa gtgattttag
    19021 caattcactt acctaaaaat agctttaact aataaaattt caattcaaga aaatgctttc
    19081 aaatgctaaa tagatacatt acaatagctt tagcaaacaa aaaggagaat atttttatgg
    19141 gaatattagt ttttgatttt ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag
    19201 agattaaagg taagggacaa tttgcaacgc ctgaatcttg ggaagaaatg aaagcgcaat
    19261 tatttcaagt ttataaaaaa atactcgttt cttttgaagt cgaaggaatt ggaatcagct
    19321 ctcctggagt agtgaataat gagaaacagg tgattgaggg aatcagtgcg attccttata
    19381 ttcatggatt taatattttc cgagatttgg aaactctgtt tcaacttcca gtaacgattg
    19441 aaaatgacgc caactgtgcc ggaatggctg aattttatga aggtgctgcc cgcgattatc
    19501 aagatgttgc ttttgttgtg gcaggaactg ggattggcgg ggcgttgttc acgaaaggac
    19561 aaatcaataa aggggcacat ctttacggtg gagaatttgg gttgatgttc ttaaatggcg
    19621 ataagacatt cagtaaactt ggtacagcgg tacagatggc ttggcgttat tgtgaacgca
    19681 aaggtctcga taaaaatgct tataccggaa aagatgtctt tgaattagca gaaactggag
    19741 atgcaatagc aaaagaagaa gttgagagtt tttatacgta tcttacaaag ggattattca
    19801 gtattcaatt ttcgtttgat ccagaagtga tcgtattagg cggcggggtt tctgcaaaag
    19861 aagggctcat tgatgagatc aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc
    19921 atgatttcga accgcagatt ttattatgtg aatacagaaa cgatgctaat cttgtaggtg
    19981 cagcagcaaa ctatctcgct gtacgtcaag gggagaatta agagctttag agaaggtgca
    20041 gcttgcataa aagaagtgaa tgaaatgaat aggagatttt tacctaagga atgttttatt
    20101 tgctgcagta tgataaaata tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat
    20161 tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt aagaattatg aagtcactca
    20221 gttatttata gggaaaaaaa tgattggtga gatcttagcc tatggaccaa aagaatatga
    20281 aattttcttt ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg cgatagaaac
    20341 agtgattcga cactggaatc tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg
    20401 gttgcaaaaa tctggaaaat taggtattat aactaagtca ggttgataca aacctttcgg
    20461 aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc gttccttagg gttcagtggt
    20521 tcgaatccac tctcctccat ttcattgggg tatagccaag cggtaaggca agggactttg
    20581 actccctcat gcgttggttc gaatccagct accccagtat accacaatcg taagctctcg
    20641 ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt ctgctgattt
    20701 aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg aaagaaaaag
    20761 tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac aatattgaag
    20821 tgaatattcc gaaaaaaggc ggaaaagcgg tccgggtaaa atcaaaacgc ccaacgaatc
    20881 atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat aagtcggcat
    20941 tttcatcgat tttagggtct tttgttattt tctaggttgg acatatctaa aaaattgttt
    21001 ctgacaatag gagtaggaag aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc
    21061 tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa acgaaaggac
    21121 gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac caattaaaaa
    21181 atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa agagaattag
    21241 cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac ttggcagatg
    21301 aaggaatctt agaaagaaag atcggttcgg gtacatatgt tgcacgagaa aaagtacaag
    21361 aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat cgagtaccgt
    21421 ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa
    21481 acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc
    21541 ctatttgttt tgaagttgcc agtgtgccac aaaagctgat cgatggctac agcaaagcag
    21601 agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag atcggtccag
    21661 cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat ttagaggtaa
    21721 aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac ggtacgccgt
    21781 ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg gaaaaataaa
    21841 aaacaggtga tccctgattc cgtattagaa ataaaagaga atcagaatca cctgttttta
    21901 tatatgatca gttaccagtt gctcgtgata agaaacagga ccctcttcaa attggacttg
    21961 tccgttcagc agctcaatga tggattcttt aaagtggtca gcactttttt catctacagc
    22021 acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat gttggagttc
    22081 aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga tctcctgttg
    22141 caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat gagtgtatgc
    22201 tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga ctactgcagc
    22261 tacattgatc agttgatttt ttttgaggac ttccaacatc ggtatgcccg ccgttccact
    22321 tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga tgaaggcgct
    22381 gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaac tttttgcttc
    22441 gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga tctcgatctc
    22501 actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac ctcttttttc
    22561 attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa cggttgcgca
    22621 attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg caggaacgaa
    22681 tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct caagtaacgt
    22741 tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc aaaacagcaa
    22801 gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt gcctatggct
    22861 ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat gtcgtgttca
    22921 ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag aaaacgttga
    22981 caaatttctt tctttttata taatagagta acagacgcga agaaaacgtt tgcgtagttt
    23041 gttttaaaaa ggagagaaaa gtatggctaa aaaaacaatc atgcttgtat gttcggctgg
    23101 tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa aaggaatcga
    23161 ttctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa ataaagacgt
    23221 ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg agaaacgtct
    23281 ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta tgatgaatgg
    23341 cgaaaaagtg ttagatcaag caatcacgct cataggtgaa taaatagaac agtataacta
    23401 tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt tagggcgaat
    23461 aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc aatagactgt
    23521 gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt gagcaactgg
    23581 attctttgtc atcgctgtgg aatgagaacg tccgtcattg atggacagct tgcagattac
    23641 ggttattttt gtacacattg tctatattta gggcgatgcg acactcaaca agatttatat
    23701 ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac aggacagctt
    23761 actgaaaaac aaacggaaat ctcaaagaga atactttgcc attcagaaaa aagacatcat
    23821 cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat tttagtggaa
    23881 actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt ttgtaatgaa
    23941 ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct ccatgggaat
    24001 acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca gctataccgt
    24061 ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc ttataacggg
    24121 gatgcaggat tagctcatgc tgtgcaaaca gcgattaaac cagacggacg attcatctac
    24181 ttaagtgcca cacctgataa aaagttacta aaggagatca agcagacgtt tgacatccac
    24241 aaacttgctt tacgctttca tcggcgtttg cttcctgaac ccgtcttggt tttcatgaat
    24301 aattggcgac aaaaatgcaa gaacaagaag aagataaaat ctttggtgcg gatcattcat
    24361 caattaatta aagaaaacca tatcctttta ttttgtccaa gtatcacgat gatgcatcgt
    24421 ttgaaagcca cacttcaaga ggctcttccg agatatgaga tcacagatgt ttcttcaaga
    24481 gacaatcagc gggcggaaaa agtccagaaa atgcgagaag caaaatatga catactgtta
    24541 accacgatga tccttgaacg tggagtcaca tttgaaagaa tatccgttat cgttttagga
    24601 gcggatcatc cggtttttac taaatcttct ttggtacaaa tcgcaggaag agccgatcgt
    24661 aaagggcctt atacgaacag tcaagtatat tttttctatg aagaaaaaac tgcagctatt
    24721 cgaaaagctt gtcaggaaat caaagaaatg aatgaagaag ggaagaagat actgtgaggt
    24781 gcagttgctg cagtaaagat atcgtccaaa atttaacatt cagcgagatt attggtgttt
    24841 cgaaatacag attacctcag acttgttcag tttgtcgcgc acaatttcat ttattggaaa
    24901 caagaggctg ttctggctgt caaaaaccaa tccatttcaa tctagagagt acgaaaaaga
    24961 aagtaaattc tatccccgtt tactgcagtg actgtcaaga atggaagaag aaatatccga
    25021 cctatgattt tcagcaccgt gccttttttg tgtacgatga aaatatgaag gcctggctca
    25081 atcaatacaa gtttatggga gatatccgac tagcaggcac ctttgcatat aaatggacgc
    25141 agataaaaaa agaatacccg acatacatct attgtccaat acctttatct gaaaaaaggc
    25201 gaacagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaga attccttttc
    25261 agatgctact caaaagagag aagcatctgc tggctcaagc aaaaaacacg cgaaaggaaa
    25321 gattggagat gcctcaacct tttgaacttg cagtcacctt ttctaagata caaaataaaa
    25381 aaatattgat tattgatgat gtctatacaa caggacagac tcttttccac gcagcagatt
    25441 gtttgcagat cgcacagccg aaaatcatcc gaactatttc tttggcgaga tgaattagat
    25501 gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata atagaattaa
    25561 gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg ggtaattttt
    25621 atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat tcgcgactac
    25681 gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga tgcaacggcg
    25741 catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac gatcccatta
    25801 ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag tatcgatttg
    25861 gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa tcgtaaatcc
    25921 cgtgaaacgg gcatgaatac tgcaaatgct gctgctttat tcaatgaaga ggacgaaaac
    25981 gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa accaatggat
    26041 agtgaagaag cagtcttgca aatgaacatg ctgggacata acttctttat ttttgaagat
    26101 gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata cggactgatt
    26161 gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc tactgacagc
    26221 tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt
    26281 tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt
    26341 aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca
    26401 atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg
    26461 caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg
    26521 caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc
    26581 cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta tatccatacc
    26641 acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa atgcgtactg
    26701 gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg actggcgaag
    26761 gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg
    26821 aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa tcttcggatg
    26881 aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa ttaggatttg
    26941 actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt ccgctgaatt
    27001 atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg ccgctgatca
    27061 tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac ttcgtgaaac
    27121 gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc agtttaaccg
    27181 aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac gacattgaaa
    27241 atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc atgcttttag
    27301 atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc actggtcgga
    27361 tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct aaagaaggcg
    27421 tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac tatttccgta
    27481 tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa gaattcagag
    27541 aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc cgtgatgatc
    27601 gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg caagatatca
    27661 aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt gaaacatctg
    27721 agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg aatgcgaaaa
    27781 atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct gtgacgattg
    27841 ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta agagaacttg
    27901 gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc
    27961 gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg tcattggaag
    28021 atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat cggatgaaga
    28081 tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta gaatctgcac
    28141 aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa tacgatgatg
    28201 tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc atggaagaaa
    28261 aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt gtagtagatg
    28321 cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat tttgccggca
    28381 gtactcttgt ccatgaagat agtattactc ttgcagatat cgaaggaaaa acagctcagg
    28441 agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa gcggctcaac
    28501 taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt gttgtggact
    28561 ctaaatggac agaccatatt gacgcaatgg atcaattacg tcagtcaatc ggcttacgtg
    28621 cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag atgtttgaag
    28681 atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct gagatccgtc
    28741 aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa gacggagatg
    28801 ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaac aaggtcggaa
    28861 gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat ggaaaaaatg
    28921 caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga attcgtctca
    28981 ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa gtatagtgaa
    29041 agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa tgcaggaaaa
    29101 ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac atcgcggaag
    29161 cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca caaaaagtga
    29221 tcagtgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt gccgaagaac
    29281 tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca gcaatgcaag
    29341 aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat gaattatcta
    29401 tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat ccaggggctg
    29461 gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca cgttgggcag
    29521 agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa gcagggatca
    29581 agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa tcagaaaaag
    29641 gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt catacttctt
    29701 tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa atcaatccag
    29761 atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac atcaataaaa
    29821 cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct agtcaggcac
    29881 aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct aagctttatc
    29941 aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa caaaaagaaa
    30001 tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg gtaaaagacc
    30061 atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac cttgatttat
    30121 ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa ttgaaataaa
    30181 attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta aagactgaaa
    30241 aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt actcaaatgg
    30301 tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg tctatgtggt
    30361 tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag aagaaaaagc
    30421 ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa accgcgaagt
    30481 tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat taccaagaaa
    30541 aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac ctcgagatat
    30601 caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag ttcgagtctt
    30661 tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg caatcgtgaa
    30721 tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag aaaactcatg
    30781 ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg tgatggctac
    30841 acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg aaaacggccg
    30901 cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc tttttccgcc
    30961 atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt gcttcagtca
    31021 gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc atgaatgcaa
    31081 ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta gatatcggta
    31141 caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat catgttaaaa
    31201 aagttgaatt ttcaagtaaa gaacaggaat tgaaaaaaat ccaaaacgaa atgggtgatt
    31261 cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta gtaagtgcta
    31321 ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag aatgtttctc
    31381 gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt gcagttcgga
    31441 catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc atttcgaaca
    31501 cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt ttagtcggtg
    31561 caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc ggtctgatcg
    31621 gtgcgattct tccgattttg atcatcacat ttggttatgc atggtttttc caattgatca
    31681 atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc gtttggaaaa
    31741 tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg atcatttcta
    31801 tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag ttggacagtt
    31861 tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct tttttccctt
    31921 tactttaaaa aacaacttgg gtattcttaa gttggtagag gaagaggtga agccgtgaaa
    31981 attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct ttcctttatc
    32041 atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct cgttttgatg
    32101 ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat ttccaaagag
    32161 aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat tgaagaagac
    32221 aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt agatgtgaaa
    32281 caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa aaatgaaatc
    32341 actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat caagcaagca
    32401 gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg tgaagaagtt
    32461 cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct tcttgatgca
    32521 tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc aggcggagaa
    32581 atcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa ttatcgcaat
    32641 catcgtaaaa tcgtagttat tgatgggaaa atcggatata caggtgggtt caatgttgga
    32701 aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca tttgcgtatt
    32761 tacggagaag cggtgtatat cttgcaaaac cgttttttga tggactggaa ttctcagcat
    32821 acaaaagaag taggctattc tcccaaattt tttccgtcca ttcactctac tggtgatata
    32881 gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa aatgacttat
    32941 ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta ttacataccg
    33001 gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa agttcatatc
    33061 caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc tttcgctgca
    33121 gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat tcatgcaaag
    33181 acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga tgttcgttca
    33241 tttcgcttgg actttgaggt caatacactg atttatgatg aacgaatggc ttcacgtgta
    33301 agaaaagcat tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga aatgtatgaa
    33361 aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc gccgctcctt
    33421 taaaacacaa aaaggacatg agaaccatca acagatggag atctcgtgtc cttttctaac
    33481 ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag cgaaattaac
    33541 tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat gctattaaaa
    33601 gagacacatt tcaaagccag tatagcatag aaacttttaa tgagatagtt ttttttacaa
    33661 ttatgaggat ttattttgtt atttaatgat tttgattcgt tttcatcaag taatgaaaat
    33721 gataaaataa acatacttta ggattaaagg agatttctaa tgaaaaaact taaattattc
    33781 ttaccaataa taggattagc tattctttta tcaggctgtt caaagtggat tgatcgagga
    33841 gaatccatta cggctgttgg ctcttctgct ttacagcctt tagtagaaac agttgctgaa
    33901 acttaccaga cagaaaatcc tggaaaattc atcaatgtac aagggggcgg aagcggaact
    33961 ggtttgtctc aagtacagtc aggagcagtc gacatcggaa actctgattt gtttgcggaa
    34021 gaaaaatcgg ggatcgatgc ctcagcatta gtcgatcatc gagtagcagt tgttgggatc
    34081 actcctattg tgaacaagga tattggcgta aaagacatca gtatggaaaa cctgaaaaaa
    34141 ctatttacag gcgagatcaa aaactggagt gaagtcggtg gaaaagatca gccagtagtt
    34201 atattgaacc gtgcttcagg aagcggaacc cgtagcacgt ttgaaaaatg ggtattggac
    34261 ggagaaacag ctgttcaggc acaagaacaa gattcaagcg gaatggtgcg ccagatcgtt
    34321 gccgacacac ccggtgcaat cagctatgtt gctttttctt atgtttccga tgatgtagat
    34381 acgttgaatg ttaacggtgt agaaccgaca gatgaaaatg tgacaacgaa caaatggacg
    34441 atttgggcat atgagcatat gtacacaaga gatcatccaa aaaaactgac aaaagaattt
    34501 ttagactata tcttatctga tgaagtacaa gataacatcg ttggagaact aggttatatc
    34561 cctgtttctc atatgaaagt cgaacgtgat tggcaaggaa atatcgttaa agagtaatct
    34621 ttacacaaaa tttacaaact tagctaaact tttacatgtt attaaaacca aattgatgtt
    34681 caatttgtac actgagtgag tatgttaaac ctgattaagg gggagtcatc tgtggaagat
    34741 gtgaaaaaag tattattaac aaaatcaaaa cgttcaaaaa tggaacagcg tggaaaattc
    34801 atcagttttt tatgtatcgc gctgatcgtg ttggttgtat tatctatttt ttattttgta
    34861 gctagtaaag gattggccac cttttttact gataagatca atgtatttga ttttctgttt
    34921 ggtacagatt ggaatccaag ccaaatcgga gcaaatggga aaccaatggt aggggcattg
    34981 cctatgatca caggttcgtt tattgtgacc tttttatctg ctattgttgc cacaccattt
    35041 gccattggtg cagcagtatt catgacggag atctctccta aaaacggaac gaagatctta
    35101 cagccggtca tcgagctgct tgtaggtatt ccttctgtcg tttatggttt tatcggttta
    35161 tcagttatcg tgccttttgt cagaagtatt tttggcggga cagggtttgg tatcttagct
    35221 ggtacatttg tgttgttcgt tatgatcttg ccgactgtca caacgatgac tgttgatgca
    35281 ttaaaggcag taccacggca ctatcgggaa gcttcattag cgcttggtgc cacaagatgg
    35341 caaacaatct acaaagtcgt gctacgagca gccgtgcccg ggattttaac agcagtcgtt
    35401 tttggtatgg ctcgtgcgtt tggtgaagcc ttagctatcc aaatggtcat cggtaatgca
    35461 gcattgatgc caacaggact tactactcct gcttctacat tgacgtccat cttaacaatg
    35521 ggaatcggaa atacaatcat gggaacagtt gaaaataatg tgttatggtc attagcgctg
    35581 atcttgctat tgatgtcatt attctttaat atcgtgatcc gtatgattgg gaagaaagga
    35641 gagctgaaat aatgaacgca aaacgctcag ataaaattgc tacaggtgtt ttatatgtag
    35701 tttcggggat catcgtattg attttagcag ctttactact ttatatctta gttcggggaa
    35761 taccgcacat ttcatggtcc ttcttgaccg aaccttccaa agcctaccaa gaaggcggtg
    35821 gaatcgggat ccagctattt aactcgattt atttgctttt gatcacgatg atcatcagtt
    35881 ttcctatttc tttaggtgcg ggtatctatc tttcagaata tgctggaaaa aactggctga
    35941 cagatgtgat tcgcacgtcg attgaaatct tgagctcatt gccttctgtc gttgtcggtt
    36001 tattcggttt cttggttttc gttattcaat tccagtatgg tttctcgatt atttctggtg
    36061 cattggcttt gactgtattc aacttgcctc ttttgactag aacagttgag gagtctttac
    36121 aagcggtcca ttacacacaa cgagaagcag gattggcttt aggtttgtca cgctgggaaa
    36181 cagtgataaa agtggtggtt ccagaagcga caccagggat tttgactggt gtgatcctaa
    36241 gttctggaag aatttttggt gaagcagctg cattgatcta tacggctgga caaagtgcac
    36301 ctgcgttaga ttttacgaac tggaatccat taagtgtttc aagcccgatc agcattttcc
    36361 gtcaagctga aacattagct gtccatattt ggaagatcaa tacagaaggc acgatgcctg
    36421 atggtgtttc tgtttcggct ggcgcttctg ccgtgttgat cattgctgtt ttactgttca
    36481 acttcggcgc tcgtgcgatt ggcaaacgat tataccgaaa aatgacttca gcctaaggag
    36541 aagaagcaaa atgaaaaaat ataactggaa tgaaacaaat gtcatcacca tgccaaaaga
    36601 gaaagaagta gcactatata cggatgatct acatgtctgg tatggcgaca atgaagcaat
    36661 caaaggagtg gatctggaat ttgaaaaaaa taagatcact gcactgattg gtccttccgg
    36721 ctgcggaaaa tctacgtatc ttcgttcgtt gaatcgaatg aatgatggga tcgctaatac
    36781 gaaaatcact gggaaaatca tgtatcaagg cgtagatatt aatgcgcctc aagtggatgt
    36841 ttatgagatg cgcaaacgga ttggcatggt ttttcaacgt cctaatccgt tcagcaagtc
    36901 gatttatgac aatattacat ttgctttgaa acagcacggc gaaaaaaaca aaaagaaatt
    36961 agatgaaatc gttgaaacaa gcttgaaaca agctgccttg tgggaccaag taaaagacag
    37021 cttggacaaa agtgccttgg ctttatctgg cgggcaacag caacgtttat gtattgctcg
    37081 tgcgatcgcg atgaaacctg atatcttgct gctagacgag ccggcaagtg ctttggaccc
    37141 tatttcgaca ggaacggttg aagaaacact tgtaaacttg aaagaagact atacgatcat
    37201 catcgtgacg cataacatgc aacaagcggc tcgtatcagc gactatactg cctttttcta
    37261 tctaggaaaa gtaatggaat atgacaaaac taacaagata tttaccagac caaaaattca
    37321 ggcaaccgaa gattatgtgt ccggtcattt tggttaagac ggaggcctca acatgacaaa
    37381 agaaattatt tcatctaaag atcttcatct gtattatgga aagaaagaag cactaaaagg
    37441 gatcgatcta agtttcaatc aaggggaaat cactgcgatg atcggtcctt ctggttgcgg
    37501 gaaatccact tatctgcgtt ctttgaaccg gatgaatgac ctgattccga atgtaacgat
    37561 caccgggagt gttttatata aagacaaaga catctatggt ccaaaaacag atgttgttga
    37621 actaagaaaa gaaatcggca tggtattcca gcagccaaac ccatttcctt tttctatcta
    37681 tgaaaatgtc atctatggat tgaaattaaa aggggaaaaa gataaaaaag tccttgatca
    37741 agtagttgaa gaaagcctga aggcagcgtc tgtttgggat gatgtgaaag acaaattgca
    37801 taaaagtgct ttatctttat ccggtgggca gcagcagcgt gtatgtatcg cacgtgtttt
    37861 ggcagttaat ccggaaatca tcttattgga tgaaccaaca agtgcgcttg atcctgtttc
    37921 tactgggaaa atcgaaagta tgctgttaga attaagagag cggtatacga tgatcatcgt
    37981 gacccataat atgtctcaag cttcacgtat ttcagataag acggcattct ttttagatgg
    38041 tcacttgatc gaatacaatg atacgaagaa gatattcatg aatccagaaa aacaagagac
    38101 agaagattat atttcaggtc gatttggata aggaggaaac ggaatgctac gtacacaatt
    38161 tgaagaagaa ttgctgaatc tccataatca gttctacgaa atggggatga tggtgagcag
    38221 cgccgtgcac aagtcagttc gtgcatacat caaacacgat aaaatcttag cccaagaagt
    38281 tatcgaaaac gatataaata tcaataacat ggaaacacgc ttggagaaga aaagttttga
    38341 aatgatcgca ttgcaacagc cagttacaac tgatttgcgt atgatcatta cggtcatgaa
    38401 agctagctcg gatttagaac gtatggccga tcatgcggtg tctgttgcta aatccacgat
    38461 tcgcgtaaaa gggcagacga gaataccgga aatcgaaaaa gagatctccg atatgtccga
    38521 ttatgtgaaa aaaatggtgg acaacgtcct agttgcttat gtcaaaacag acgaaaaaga
    38581 cgcacgtacg atcgccaata tggatcaaag agtcaatgac tatttcaatc gaatctataa
    38641 tgcaacaatc aaaaatatgc aggaaaatcc tgagacagtc atcagtggaa cagactactt
    38701 gaatgttgcc agttatctag aacggatcgg agactatgtg acaaacatct gtgaatggat
    38761 cgtctacttg gctacaggga aaatcactga gttaaatacg aaccacaatg agattttcta
    38821 aactaaagcg gtgattctgc cgtttgttta ggaaaagagg ttgtgaaatc agcggttgtg
    38881 aaaaagctgt tctgcatcaa gtaataagaa cagtcaaaca aaaatagctc ctcatatttt
    38941 tcatttggct ggacttatta ccgcagatgc aagcttttga acactgttta ttcgggaaag
    39001 ggtttgtgac aaaatatgtc acaaaccctt ttcatcattt catagaaagg ataaagaatg
    39061 caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc atgggttatt
    39121 cagcatcaag ctgctttata tgaagtacct gtcatcgaga tcgaaaaagt acgaataaaa
    39181 gaggatcatc aattgattac aggaagactg atgaacacga atcaaaaagg agaatcgatt
    39241 tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa agcgagacaa
    39301 atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa aaatgatggc
    39361 tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg cggaaaacat
    39421 ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc
    39481 atctttacta aaatagaagg gatttctcta ttaaatatat ctatcctgtt cgtcgttata
    39541 gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt
    39601 tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatctt
    39661 tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct
    39721 gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca
    39781 gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta
    39841 tggctttcgg gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg
    39901 ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtcg
    39961 ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc
    40021 ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt aaggaggctg
    40081 aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg
    40141 gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg atctttttct
    40201 tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt
    40261 tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag
    40321 cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt
    40381 ttttagcaat ccaaggtttt acaacgatag aactagaaga attatctggc ttagataaga
    40441 ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg
    40501 ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc
    40561 cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg
    40621 cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct
    40681 ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag
    40741 aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct ttaacctgcg
    40801 tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac
    40861 aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct
    40921 tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt
    40981 tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa
    41041 ctgaaaaaga cgaaaagcaa ataaaaaaag aagcagacaa ggtaactgct agtcataaag
    41101 aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg
    41161 agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa aagatcttag
    41221 atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat
    41281 tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa
    41341 aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg ttagaagcag
    41401 aaatcaaaga gttagaagct agtggcgata cactgttaga agaacaattg aaattagaag
    41461 cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata
    41521 agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg
    41581 aaaaaatgtc agaagctgga tcacagttag gaaaattctt gaagaaaaca ttccaaactg
    41641 tttcagaaac agtgaatgat aacatggaat ggaaagatgt cagcttacgt gtccctggga
    41701 ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg
    41761 atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag
    41821 ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg
    41881 aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg
    41941 tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac
    42001 ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta
    42061 cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa
    42121 acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg
    42181 gtacagtgac atttggtgcg acagcagaaa atctttctgt gtctttagta aatggtgatg
    42241 tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa
    42301 atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg
    42361 gaagtatcaa tagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa
    42421 accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa
    42481 cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg
    42541 ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg
    42601 tgccttattg ccgataggct atctcttgaa caccgtttac caagagatta tgaacttgcc
    42661 acttatcgcc gagtcacaag gaataatttc aaaaaaagct cgtcacgttt tatgaatatg
    42721 gtaatttgtg accgagaatt ttgcagagag acaccgatta agaagaaggt tgtcacaacg
    42781 attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa
    42841 atccaataaa aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat
    42901 tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc
    42961 aatcatttta tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa
    43021 ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca
    43081 aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg
    43141 tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt
    43201 cctgcgatgg tccatgtaag cagtatttgg acggccgttc ttgccagctt cgtattgtct
    43261 gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc
    43321 acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt
    43381 gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg
    43441 atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg
    43501 ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt
    43561 aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga
    43621 tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca
    43681 attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt
    43741 catcgtaact ggggacatct cacgcccagg actagagttg actggttatt ttaattatta
    43801 ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat
    43861 cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat
    43921 tatttcaaga ggattaccgg ctccagaaga aatgatccag gcagcaaatg aaaatgggat
    43981 agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt
    44041 agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt
    44101 aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat
    44161 caaacgcggt catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt
    44221 aacggtgatc ggagaaccgc ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg
    44281 gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca
    44341 gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga
    44401 agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac
    44461 aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat
    44521 gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc
    44581 aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag
    44641 cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgcc
    44701 atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat
    44761 tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta tgttttattt
    44821 gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta
    44881 gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat
    44941 tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca
    45001 atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc
    45061 ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc
    45121 tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt
    45181 ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta
    45241 atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt
    45301 ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt
    45361 ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa
    45421 aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa
    45481 aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt
    45541 cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga
    45601 aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac ccgattccat
    45661 ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat
    45721 tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc
    45781 agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat
    45841 cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag
    45901 ccatgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa gtacagatgc
    45961 aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa
    46021 tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc
    46081 tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg
    46141 attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgttga cctttatcgg
    46201 attaagcgga gtaggcgatc tagtcgttac ttgcacaagt gttcattcta ggaactggcg
    46261 cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca atatgggaat
    46321 ggtcgttgaa ggtgtgaata ctactcaagc agcgatggaa ctagcccaaa taatggaaat
    46381 cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag aaatcaaaac
    46441 agccgcaaaa gaaatcatgt tacgtgatgg gaaaaaagaa aatgaattct cattaacaat
    46501 tttttgaagg agcgaatgta tatgaaagtc aaaaaagcag ttattccagc agcaggatta
    46561 ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc aattgttgat
    46621 aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggaatcga agacatcttg
    46681 atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa tattgaatta
    46741 gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga agaaacgaca
    46801 gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca tgccgttctt
    46861 caagcaagag cctttattgg aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg
    46921 gaagatgaag taccattgac acagcaatta atcgatgatt acgaacgtac tcatgcttct
    46981 acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa aatacggaat tatcaatcca
    47041 ggagaagtat tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa accagatcca
    47101 agcaaagcac caagtgattt agctattatt ggacgttact tattgactcc agaaattttt
    47161 gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa tccaactaac tgacgcaatc
    47221 gatacattga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa acgttacgat
    47281 gtcggtgaca aatttggttt tatgaaaaca agtattgaat atggtctaac acatccagaa
    47341 gtaggtgcac ctttacgtga gtatatcctg tctttaggta aaacattaga agcagaagga
    47401 agcaagatcg atcataatcc aaaaggaata acagaaatca aagaagaaaa gaaaaaataa
    47461 cccttttttc taacgagctt cagctgctaa ttgaataaaa agtagttgaa gctcgttttt
    47521 atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag aagggagttt
    47581 gatcatgtca ggaggagaaa ttgcaggatt aatcgcagca atcgcctttg cgatacttgt
    47641 cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag tagatcagac
    47701 tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag
    47761 acaagtagaa ggcttgctgg tcaaaagtaa tgaactgctc ttggatatca atggaaaagt
    47821 cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg tatcagaact
    47881 taatcattct agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa
    47941 agcgacggtt gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca
    48001 tactgaaacg acaggaggaa aataaaatgg ctaaaaaagg tggattttta ttaggtgcag
    48061 tagtcggagg aacagccgca gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag
    48121 aacttcggga tgaattagct gcccaagcgg atgatttgat ggatatggct tcagattaca
    48181 cagatatggc gaaagaaaaa tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag
    48241 atctttctca acaagcaggt gatctagcag gtagtgtcaa acaaaaagta agtgaatcag
    48301 cagatgaagc aggaagtgaa tctgacgata tcttgttaaa tcttaaagaa caatcagctg
    48361 acttatctaa tcgtttcaaa aaaacggcag aagatatgaa aaaacctgca gatgaattag
    48421 gaagtattgc taaagatact tcagaagata tcatgattga tgtaaaagac gctgctggta
    48481 aagcaggaga tactgtcaat gaaggaatgt cagaagcaaa accagttgca gatgacacaa
    48541 aagaaatgat gaaaatggca aaagaagatg cgaaagaagc taaaaaagaa ataaaagata
    48601 caatgacaga ttcatctaca aatcactaaa atcacaaatt tatagaataa aaaggtggga
    48661 gcggactagc tctcaccttt tttactctta tttttctaaa acaagtaatt cttttggtgt
    48721 agtcgtgaat ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc
    48781 tactttttca ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac
    48841 taagtcattt ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc
    48901 taaacggtga ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg
    48961 atcaagttca cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc
    49021 aagagtgatg ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg
    49081 agtagcatca ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt
    49141 cggtaaaatc ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag
    49201 gactaatgtg tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc
    49261 tgcaatgatc gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc
    49321 aacatcggcc catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag
    49381 ttgtagacgt tggatgatgg aagttagatc agtgtaagaa tccgttcccg tcaagatatc
    49441 agagatagca tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg
    49501 ctgaccatag cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa
    49561 atagccacgg acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg
    49621 agtgaaaagg aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg
    49681 atcactttcg tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc
    49741 gacttggtgt tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca
    49801 acctactttc taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca
    49861 acattctaaa aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa
    49921 ttcttgagat tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat
    49981 aaaatatgaa taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga
    50041 gagaggcaaa tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtta
    50101 aaccagcaac aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg
    50161 cagttgcgcg tggcttggca agtaagaaaa caacaacagt tggtgtaatc atccctgatg
    50221 tcagcaatat gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca
    50281 aatacaatat tatcttagca aattcagatg gcaacgacca aaaagaagtc aatgtattga
    50341 ataacttact ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg
    50401 aaatccgtgg agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc
    50461 cagatgaaca agtaggtagc gtaaacattg attatgtatc cgcaacaaaa gatgcagtaa
    50521 acaaattggc aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc
    50581 ccatcaatgg acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac
    50641 catatagcga aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag
    50701 tcgatcggat ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca
    50761 tcggtatttt ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca
    50821 tcacaagtaa caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac
    50881 aaccattata cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag
    50941 aagaagtcga agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa
    51001 aataataaaa ggacaaccat tcagaggttg tgtgcctgcc gtttaactct gcgttacaag
    51061 gagcaatttc aaaaaacagc atctcatgtt tgatgaatat tgtgactcgt gattcggagt
    51121 tggcaggaga acaccgttta tttgggaaaa ggggttatga caaaattttg ttataatcct
    51181 ttttttgtgt cttaaaaaag agagaaagtg gttgaaatta tatttatcgt atataaaaca
    51241 atccgtgtat agatgctgta ttttgttcac catcatcaaa aaaataaggg aatgtgacaa
    51301 tactattgtc acattccctt attactgtgt attctattaa atcaatcatc tttctcatct
    51361 gattctttgg ttgcttgatt cgctcttgca taagctgaag cggtgttcca gtaatctgta
    51421 tagttttcat cggtaccgcc aacgccaaac ttaaaggcgc ttttcttcgg tccatttttc
    51481 gcccatagcg attcaactgt ttttcctggt gtttggatgg attttttatt gacagtaact
    51541 tttccaggtg tttgaccagt aaatccagat acttttttct tcatcacatc agatgataat
    51601 tcgaatttct catcagtgcc aaaaatgtca ggttttgctt ggtaagcttg attgatcaga
    51661 ttggcaagat aattagccga acgtatcctt gctttgctgt ccattccagt attgtcatcg
    51721 tgccctgccc agctactgat ggtaatagac ggagtagaga caattagcca tgaatctcgg
    51781 tattcattgg tagaaccagt ttttccgacc caatctgctt ttcctagatt tccgtttaag
    51841 cttgagatgg catctttgaa tggtgtagtg atttttgcat tgatcacact gcgcatcatg
    51901 tcattcatga tcgatgccgt tgcttccgaa tagatccgga cagagttgct ttcgtgttta
    51961 taaattacat ttcctgcgtt atctgtgatg gaatcgatga tatagccttc ttcatagacg
    52021 ccgccgtttg ctaaagcctg aaatccgttg gtctgttgaa gcgtcgtgac atctactgta
    52081 cctaaaggag cagattcaac gccccaattg tcatttgccg ggtagttcat ctttgacaag
    52141 tagtgctcgt aagcatattg tttcgatcca ccggaattca acacatcttg atataagtga
    52201 taagccggga tattgttgga ccattctagt gactcacgga cggtttggaa ggtgttcgat
    52261 ccttcgttag tagcattgac gattttctcg ccagcatttt ctccttcttg ccaagttgtt
    52321 gcataatctg atacacggga ttctgatccg ataagcccca tgtcgattgc aggtccgtaa
    52381 acaagaactg gtttgattga tgagccagct tgacgttctg tatcgaaggc atggttattt
    52441 tgattctgac tgtaattccg tccgccaacg aatccgtaga tgcggccagt tctgttatcc
    52501 atcaatacat ttccggtttc tacttgactg ccagcaccat catctaacat gtatccataa
    52561 ttttcaacgc cattttgcaa tgcagcatag acatctttat cgattgtcga atggattgta
    52621 tatcctttat tttggatcgt ttgctgtgcc ttttgataat aagcgtcact gatcgatgaa
    52681 tcagacatat cagcgttgtc tttttcagcc aattgttgtg caatgattct tgttgcttca
    52741 ttcatcacag tataatacaa gaattctctg tcagtctgtt ctgcgatttg ttgtggtaga
    52801 aagtcttttg taaggtcata ggcttttgct tcttcgtatt gttctttcgt aatttggcct
    52861 tcacgataca tactgaataa aacaaaatct tttctggcaa ggccggcaga aagatcttct
    52921 tttagcgcac ctgtgttcgt gtatggacta tacgtgatcg ggctttgggg taatccagcg
    52981 atataagcag cttgcggcag agtaacatct tttgcatcta caccaaagat tcctcttgca
    53041 gcttcctgga ctccagcaat gttttgtccc ttattattac gaccaaatgg tgagacattc
    53101 aaatacgtag caatgatctc atcttttgaa aagtattttt ctacttgtgc agaaagaatg
    53161 atctcgtttg ctttacgttt gaatgttgtt tcatctgtaa ggatctgctg tttgactaat
    53221 tgctgtgtca aagttgatcc accagaagag ccaataccag tagcttcaga tactaatgct
    53281 cgtaaaacag ctttaggaac gtaaccttgg tgtttatcga agtattcatc ctctgtgctg
    53341 ataattgcag ttttcaataa aggtgaaatt ttatcagatg aaatagtcgt gcgcatcaga
    53401 tctgtctgga tttttgatat ctctgtatta tctgcataaa caatctttga cgtttctgta
    53461 atgtttccta gttctgtctg cagtgctttt ttagtcggca attgtgtgtc ttcgacgaga
    53521 taggcaaaat agccagcgcc tattccaata cctagagctg cgaacatcaa aagaagaaca
    53581 gtaataaaaa cagtcaatga ttgaaaaact ctaaaaataa tcttaggaac aaaccatttt
    53641 tctttttttc ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca
    53701 actaatggtt ttatttcgct aattatagca aataatagaa caaaaaaaca gacaaggctg
    53761 tttttggcag gaatttcttt tttaaaggag ttaaaacgtg agaaaatgat cttttttata
    53821 aaagagttct taagcttttc tttagagaaa atttcagtta ctatggagaa cagggcaaag
    53881 aaaaatcaat cattttcttt gtatatcatc ttaccaatat ataggaattg ttagtaacaa
    53941 ttgcctttca aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa
    54001 gaaaatgctg ttaggagcag cagcagaagc agccagttat ggatcttcga cttttatgat
    54061 ctatacaggc gcccctcaaa acactagaag aaaagccatc gaagagatga atattgatgc
    54121 cggaaaagcg tacatggcag aaaacggtct aacaaatatc gtcgttcatg ccccttatat
    54181 cattaatttg gggaatacga tcaaaccaga aatgtttcct tttgccgtcc aatttctgag
    54241 agaagagatt gttcgtgcgg aagctttagg cgctactcaa attacacttc acccaggtgc
    54301 tcatgtaggg gcaggaacag aagccggaat tgctagaatt attgaaggct tgaatgaagt
    54361 tttgacaaaa gaacagacgg cacaaatcgc tttggagaca atggcaggta aaggtacgga
    54421 aatcggtcga acatttgagg aattagcgca aatcatcgat ggagtaaaat taaatgaaaa
    54481 attatccgtt acttttgaca cctgtcatac gaatgatgcc ggttataata ttaaagaaga
    54541 cttttcaggt gtaatggaag agtttgataa gatcatcggg ttagatcggc ttaaagtact
    54601 ccatatcaat gattctaaaa atccgcaagg ttcgcataaa gaccggcacg ccaatattgg
    54661 ttttggaaca atcggattcg acacgctgaa taagatcgtc catgatccaa tgtttgacaa
    54721 tatttcgaaa atcttggaaa ctccctatgt cggcaaagat aaaaagaaag cttacgcacc
    54781 atatggaaaa gaaatcgcca tgttcaaagc acaaatattc gatccagaag tgtttcctga
    54841 gttattagca gaagaagtga tactctaaga ttttctatct gacagcagca aaaaaggacc
    54901 aggcacattg ccttgtcctt ttttgtttaa gaaacaaagg agtacttttt gcagtctttt
    54961 tcatcatgca tcttctcccc cctcttttta aatttagaca aacaaggtat actgttagaa
    55021 aagtaaaaaa acgaaaagtt tcacaagcaa gtggcaaaac gtgattggaa ggacgaaaag
    55081 atgctgaaca ctttaaaacc cttgatcaac caactaaaaa agaaacatac atactttatt
    55141 cttggcatag ccctattatt tacaattatt cgtttgtatt tatatagtaa agcaatctat
    55201 ggaacagaca tgtcggcaac ctatgatgac cagctgctga ttcgctatgc tcaaaatatt
    55261 ttggacggta aatggctagg ggagtatacg actacaacac tttccaaagg aatctcctac
    55321 agcttatttt tagtacttgc acataaactg catctttctt atgctgtttt gttcggcatg
    55381 ttcagtattt ttgccagtat attggttgca ctcgcgttta aaccgatcgt gaagaacaag
    55441 tatcttcttt taagtatcta tttttatttt ttgttttctc cgattagttt tacacatgaa
    55501 tattccactc gaacatatcg taatgccctc gtgattccgg ctgttgagat cattatcgct
    55561 tgtttactag ctatttatta ccgcaaatac ggttctagca gaaaattgct gccatggttt
    55621 ttagggcttt ctgtcgcttt tcctttcttt tggttcattc gcgaagattc tatctggctt
    55681 cttcctttta ctgtagtagc tttgggtatc agtttcgtcc agatcttgct agggcaagac
    55741 ttttctttca aagaagtaaa gaagttaaaa aaacgactct ttcttctctc gaaacgggaa
    55801 tggctgaaac tttcattgtt tttacttcct atagttggct tttccatcgt ctatcagtgg
    55861 atcgaatata aaaatgagac aacatacggt attgcaacca tcaatgaccg ttccagtgga
    55921 gaatttggac gtttgatgaa acaactgatc cgaatagatg atgaaacgaa cttgaataaa
    55981 gaaaacagtg agatatgggt ctcacatgct gcactaaata aagcattagc cgcatctcct
    56041 acattccata ctgtttctac ccgtattgac gaattatata cgactcacgc gtggactcaa
    56101 ggtggaaaag taaaagaatt acccggagat atcatctttt gggcattgcg agatgtggta
    56161 agcgaatctg gttattacca aaacaatgca aaagaaacca atgatttttg gaaaaaagtc
    56221 aaccaagaat tagaagaagc ctatcacaag ggaaccatca aaaagaaaaa agagttctat
    56281 ctgatgggat caggagatgg aaagacgatg agtgatctgc caatcgtatt cgatttcctt
    56341 caatcaggat acgcttatgc atggcgatat aaagatttcc atcaaggagg aaattatagt
    56401 ttcggaacgc aagaacaagt caaacaggct caagaattat taaaccttcc gctaatggag
    56461 aattggatcg atagtaatga accgagaaca cctaatttcc aattaacaaa atcagcaaaa
    56521 gtcgcgaatg tcatcataaa aatttaccaa gttctggcta ttcccgtgct gattgcttca
    56581 atagcaggga tattacttat gatactagga accgtatttg caaaagctga tcgtgcctat
    56641 tatagttcct ctttgattgt ggtcaccgga ttaatactga cacaattggt ttttcttttc
    56701 ggtgtctcgt ggttttgttc ttatgcgcca gaaaaaaagg attatttttt aagtgtctac
    56761 actggcgcag gcgtgccatt aacgcaattc atcgtagtaa caagcatgct agttttcagt
    56821 aaattaaatc tgaaaaaggc tttatcttct aaataaaaag acccttggta gctagatttt
    56881 taacctaact atcaagggtc tttgtgtgga aaaatcagtt tctattcgat ttcttctacc
    56941 gtttcctgca aacgctgttc atattttttg tttcctttat ataaatcacc aatcgtccat
    57001 gccaacagga gaaggacaag aacaatgatt acataagcga tgtgatcagc caatgctagt
    57061 tgagcagaag tggcttgttc accaaagaaa ctttcgatct gtcctggtaa tcctcgtaag
    57121 ttcaagtaag ttagactaat cacagagaac cagccaagaa gttttacgat gaatgaattt
    57181 ttaaatcggc tacccatctc cgcttggctg ttggtcatca tcagtaaagg gatcatcgaa
    57241 aacggcaacg caaaagccaa gaacacctgg gattcattca tcaagttatt caaagcggta
    57301 tgttcttcga tcgttccttt tgtacttgta aacaaaacac aaatcaatac aggaatcaca
    57361 gaaagcaatc gtgtgaccag tcttcttagc caaatcggga tacgcatatg gatgaagcct
    57421 tccatgatga cttgaccggt caacgtacca gtaatagtag agttctggcc agaagcaaga
    57481 agtgctacag caaataaagt ggataagata ccagattttg ctacagcaat caagatacca
    57541 ttgctcatag ctgatggatc agataaggct tcatacagac caaaaaagga aggatcttgg
    57601 acagaaccgg ttttgaagac agcgacaccc atgatcaata gcagtgcatt gactagaaaa
    57661 gccatcgaca attggatatt agagtcccaa gtagaaaaac gtacagcttg tgctacatct
    57721 tcttcgtctt ggtgatcaat cttccgagtt tgagaaactg ctgaatgaag atataagtta
    57781 tgcggcatga ctgttgcacc aataatccct aaagctccag tcagcggagt ttcgccgccg
    57841 atgaccggtg agtctgagaa tgtatgactg ttagggataa agccagcaaa taaactgtgc
    57901 caatctgggt tagacaagcc aacttgatag acaaaaacga ataaaatgac gaaaattaag
    57961 cagacaacca gtgcttcgat cttacgaaac ccgacttttg tcagtagtaa gagaacgaaa
    58021 acatctaata cagtaataaa gacggcaatc accaatggaa tatcaaaaag taagtagagg
    58081 gcgattgctg caccaatgac ttctgcgatg tctgttgcca taatggctaa ttcagtcaag
    58141 atccataaaa tgacacccaa tgtcttactt gtgcgtgctc ggattgcttg ggccaaatcc
    58201 atttgggtca cgattcctaa ttttgctgcc atatactgta aaagcatagc aatcagacta
    58261 gacatcaaaa taactgacat caataaatat tggaaatttt gtccaccagt gatcgaagtg
    58321 gaccagttac ctggatccat ataaccaact gctactaatg cacctggtcc agagtaagca
    58381 agaagtgttt taaaaaaact tttccccttt ggtacatcaa ctgttccatt tacttcagct
    58441 aaagataaac catttgtatg ttcaataagt cgatgcttat gaaattgttt gttggattga
    58501 ttattcaaga aaaaaccacc tttcctattt atctacgatt acattctaca cctcaaaaag
    58561 aaaaaattaa agaaaaagat tcggtgtgcc taatttattt tcgtggcgcg tcttgttaga
    58621 tgacgaaaga aattttgttc tcctctaaaa aaaccactat tttttgcatg aaactaaaga
    58681 atacgccttt cttttttcaa taaactttag taaaataagt cattgagagc aattcggaaa
    58741 tagaaaggag agatttatat gttatcaaca ggtattgtcg tacttattgc tattattgct
    58801 ttgctaatcg gcgcagtagg tggctttttc ctagcgcgta aatatatgaa agactattta
    58861 gaaaaaaatc ctcccgttaa tgaggacatg ttgcgatcaa tgatgatgtc aatggggcaa
    58921 aaaccatctg agaagaaaat tcgccagatg atgcagcaaa tgaagaacca agggaaaaaa
    58981 taggttctga cccaacgact ttttacgggc tgattttttc agcccgtttt ttatttcgta
    59041 atttgttaga ctattcagat tattcagcaa aaaagacaag cacagacaat cctaggctct
    59101 tgataaagac aaaggaggag taaaatgtcg atttttagaa agttaggctg gttttttcgt
    59161 caggaaaaga aacattattt aattggggtt ttctcgctat tcgcagtggc cttggtacag
    59221 ttagttcctc caaaagtgat tgggatcatc attgatgaga ttgctgataa aaatttatca
    59281 gtaaagatca ttttcgggtg gatcttccta ttgattatcg ctgctttttt acaatatctt
    59341 tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcggttgga gaaagaactt
    59401 cggcagcagt tattcaaaca ttttacagaa atggacaatc tcttttacca aaaatatcgg
    59461 actggggatt tgatggctca tgcaacgaat gatttgaatg cgatccagaa tgtagcagga
    59521 gctggtatct tgacctttgc agattcagtg attacgggcg gaacaacgat cattgcgatg
    59581 attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc tttgctggcc
    59641 gtcacttcgc gagtgctggg gtcaaaactc catgatgctt ttcgtgattc gcaagaagct
    59701 ttttctagta tcaatgataa gacacaagaa agtatcactg ggatcaaagt cattaaaaca
    59761 tttggacaag aaaaagaaga tctagcggat tttgaagaaa agattgaaga tgcaattatt
    59821 aaaaacaaac gagtcaattt tttagactcc ttatttgatc cattcattac cttgattatc
    59881 ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga aggcacgatc
    59941 tcgatcgggc agatggtctc atttattagt tatatcggta tgcttgtttg gccgatgttt
    60001 gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga tcgcgtcaat
    60061 gaacttttgc atgaaaaaac gcatatcgtg gaacaacttg gtgcagtgca gaccccggca
    60121 aaaggcgagc tgaatgtagc tatttcctct tttaattatc cgaaagaaga atcaccagca
    60181 ttagaacaaa tcaagtttgc aatccaagaa ggagaaacat taggtattgt tgggaaaact
    60241 ggtgcgggaa aaacgacgat catcaaatta ctgatgcgtg aatacgatga ttatcaaggg
    60301 aaaatccaat ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt attaggatca
    60361 atcggctatg tgcctcagga tcacttcttg ttttcgatga ccattcgaga taatatccgt
    60421 ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt agcatttatc
    60481 aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga acgtggagtc
    60541 tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat caaaaatcct
    60601 gaattattaa tattagatga tgcactttct gcagtagatg ccaaaacaga agaagctatc
    60661 ttgcataatt taaaagaaat aagaaaagag aaaacaacta tcatttcagc tcaccgttta
    60721 agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat cattgaacga
    60781 ggaacccatc atgagctgct tgcactaggg ggctggtatg aacaaatgtg ggagaaacag
    60841 caattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat caatcggaat
    60901 ggacaaaatc gattccactt aaagaacaga aaaagattat caaacgactg atgaagttcg
    60961 ccaaaccttt tagaagaacg ttcatcgttg ctatcttatt tgcgtttgct ctgtctgtga
    61021 tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg gctaaacagt
    61081 cggctacgac acaagttatt ttgttttttg ctggtatgta cttatttgga gtcatagtca
    61141 aaagtatcat ttggtttttc caatggtatt tgtattccat ggcttcattg aagacctatc
    61201 agtatattag ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt tattttgatc
    61261 agacaccggc aggatcaacc gtttctcgag tgacgaatga cacagaaacg ctatttgaat
    61321 tttggtacgt ctttttgatg gtattgactg ggatctttgc agtcgtttct tcattcattg
    61381 ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgttcttg ccaattttag
    61441 gaatcgtcat ctggtattac cagaaattta gttctcgtat ttaccggaat atgcgagaaa
    61501 aattaagtca gctgaatacg aaattgaatg aatcgatttc cggcatgcgt attatccagc
    61561 agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaat gagtatttag
    61621 acacgcgcta tgcgatgatc aaaacgaatt cgattctgtt aagtccaatc atcaattttc
    61681 tatatgcatt ggcgattgcg ttggtgttga ctttgtttgg gatcgatgcg ttgcattctc
    61741 ccgtagaagt tggactgatc tatgcattta cgacctatgt ccagggattt tttaatccaa
    61801 tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct ggtagtcgta
    61861 tcttgaagat tatggataca gaagaatatg caccacaaca aaaccctgga gcagatcaaa
    61921 tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat ggagagcaca
    61981 atgtattaaa caatatctct tttgttgcta atccgggaga gaccgttgca ttagtgggac
    62041 acaccggaag tgggaaaagc tcgatcatca atgtgttgat gcggttttac gaattttatg
    62101 aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa gagttacgta
    62161 agaaaatggg gctggtcttg caagatgctt tcttgtttta tggggatatc gcaggaaata
    62221 ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct gagtttgttc
    62281 aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg atcgaacatg
    62341 gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca atcgtgactg
    62401 atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa acagaaggat
    62461 tgatccaaga aggattgaca aaaatgcgac aaggacggac aacgattgcg attgcccatc
    62521 ggctgtccac gattcgggat gcagaattga ttttagtttt agacaaaggt catatagtag
    62581 aatgcgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat atgtacaaac
    62641 tgcaaagcag tggtgtgtaa aaagtggttg tgacagggct ccgttcagct ttaggtgata
    62701 agaaaaaaat tgtaaaaaag gctttccaga tttttgcata aatttggctt atcaccgata
    62761 agctggtact ggaacaccgt atattagaag ttgggacagt agcatagtag cattttgacc
    62821 taagcactag agtgaaaata aaacagagct tcccagattc ttcatctgtg agcgttagtg
    62881 tcgtaattac cagatttaaa aaaaatcacg acagctagtc agaagtgccc aatagtcgct
    62941 aagaaagcgt tggcaaaagt gcttgctttc gataatataa agaaaacgat acaacttgga
    63001 tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggttccat ggacggcttt
    63061 ttgggttttt ctttttgttt tggatacagc cctatatatc atggagagga gtaaaacatg
    63121 ctgattgaga aagtactgaa taacaacgtc gtaataacca gggatgaaag aaatcaggaa
    63181 atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc gatttttgac
    63241 tcatgcatcg acaaaaaatt tcagttgaca aatgacggac tttcacaaca gttccaagaa
    63301 ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat tgaatataca
    63361 aaagaggctc tacaagtcca gttgaatgat tcggtgtata ttgcattgac ggatcatttg
    63421 tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt gctgtatgat
    63481 atcaaacaac tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa gaaaatctcg
    63541 gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc
    63601 aatgcacaag gcattcaaag ccaacaagac aatacctacc tgtttactac aacaatacag
    63661 gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat
    63721 ttttttcgat tcgtcacaca cctacgtttt tttctgacac gcgtcctaga tggagaaaaa
    63781 tcagaagtgg gagaagtttc tgaggaattg taccaggtta ttcaaaaaaa atataaggat
    63841 acgtttggtt gcgttgagaa aatagcagac tttttagccg agatattcaa ctaccagata
    63901 tccacggacg aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt
    63961 tgtaaataaa taaaaaaagg aggataaatt aacaagaacg catgaaataa gggtagtgac
    64021 attcagttgg ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaatatcaa
    64081 gaattagcga gcaaaattgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca
    64141 cattgtatca caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc
    64201 ttgaaaaata tggacggtgt tgtgacggta atgcgagcgg gaggacagta ccaagttgtc
    64261 atcgggaatc atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt
    64321 gatgaggctg tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg
    64381 ggctgtttcc agccattcct tgctattcta gctgctggtg ggatgattcg tggactgaca
    64441 gcattacttg tcttcttagg cgcatttgac cgcggttctg gcacatttgt tatgtttgat
    64501 aatatcggtg attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt
    64561 aaatttaaag taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc
    64621 ttgtcgctgg aagctttgag tggtgcagca gaagcaccta tttatcaaac agtattcggc
    64681 attccctgga ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg
    64741 ttagctagcc aaattcagaa gccaattaaa aagttagtac ctgagatgat cgcgaatttc
    64801 ttcgttccat tcttcaccgt tcttattaca atgcccttag gttttttact cgttggacca
    64861 gcattcacat tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg
    64921 ccagttattt atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc
    64981 cattgggcaa tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatc
    65041 atgacacctg ttgcagtagt gtcttttgga taaactgcag cacttactgc actctacttt
    65101 aagcttagaa acccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg
    65161 gggattacag agccagctat ttatgggttt acattgccac gtaaaaaaat atttattttt
    65221 acctgtgtcg gtggtgcgtt agggggcgcg tatagtggat taatgaattt aacttcttgg
    65281 aatcaaggtg gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta
    65341 acggatgtga ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttgcgctg
    65401 acttttttct tttggaagga ggaagcaggt gaaacagatg atagacagaa aagatcaggt
    65461 aaagaaattg taaaaacacc gatacaagga caaatcgctc cactgaatgc agcaaaggat
    65521 gcagcttttg cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa
    65581 gtacgtgccc cttttgacgg aacagtcatg accctgttcc cggcgaaaca tgcaatcgga
    65641 atggtttctg aagcaggtct ggaattgctg attcatgtcg gattggatac tgttcagttg
    65701 gaaggaaaat attttgaaag cttggtgcag caaggagaaa gagtgaaaca aggcgatctg
    65761 ttgctccgtt tcgacaagaa gaaaatcgaa gaggaaggat acagtttaga aacacctgtg
    65821 atcgtcacca attacacgga ttatttggat attttggaaa atcaagggga gacggtcggg
    65881 ccagacgatg cacttatcac cgcactgacc tgaacggtta ggtgaaagaa tggactaggc
    65941 ttaactattc tttttattcc acttctttat tttcgaaatt gaaaggctct ttcatgatgg
    66001 ccgagctgat agggcgttta tcgaattttt gtcctacaga gattagatgg actaaattct
    66061 gttgataatt ttctgcaaaa acacaactat ataacacatc tagaagataa caaatcgatg
    66121 tattgatcgt aaaattacca attttcgaat acagtcgttc gcgtgtcgta agtcgcaaag
    66181 cggctgtact cattgaagat agcgttgaat caccgatact ggtcaaagct aaaattggaa
    66241 catgattttg tttcaatatt tctgcggtac gaaggatcac attgttttca cccgtataag
    66301 aaatcagaat cccgcacata tcactcgtgc aattgtacgc ttcatagaac gattcaccaa
    66361 aagtggtgac aatcgtgaca tccattttga tgcgcttcat tttcagcatg aaatcttgag
    66421 aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt ttttgcttga agcattagtt
    66481 gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag tgtatcttga atcgtcatct
    66541 gttctaaaga agccatttta tttgcgatcg tcataatacc gtctgtggtg gaaaaaggaa
    66601 gattcgcatc tacgtcttga aaattccctt caagataggc tgtttcatct aaaaaagcat
    66661 tacgaaaact gacccagcca tcaaaaccca ttttttttgc tacacggatg agtgtggacg
    66721 ggtggacata acatcgctca gcaatctctt ggatggtcat ttcgccaagt gctggagaat
    66781 cgcttaagag aaactgaatc acttcatttt ctgaaggaga aaaggagatt tgtttgattt
    66841 tttcacgtaa taacatgtag ataccttctt cttgtttttt tacgtaaatt ataacttttt
    66901 tgaaaatgat ttgtaaacgg atttatgtta tatagcttaa taaagacatt ttttgacaag
    66961 atatttacaa aaatccagaa gaaaacacta acttttgtaa atgttactgg aaagaaacga
    67021 atttctattg tttgttttgt ttacaaaaaa gataataaaa gtgtaaaaag caaaggagtg
    67081 agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tatacccccg
    67141 agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg
    67201 tagatgtaga agaaggaaaa gaaaagcttg agatcgtcag tcagatgtct cagcgtatgt
    67261 gggatgcttc tccgtatgaa gtcaaaatct atgcaactct ggatcgggaa gaagcattga
    67321 aagatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag
    67381 acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga
    67441 tattcaaagc ttttcgtacc gttccagtgg ttttggcaat cgttgaagac atgaagcgtt
    67501 tatgtccaaa tgcttggctg atcaatttcg cgaacccaag cgggatggtg acagaagcag
    67561 ttatcagata tggcaaatgg gataaagtga tcggattatg caatgtgcca gtaatggcga
    67621 tgatgacaga accaacaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg
    67681 gtctaaatca cttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag
    67741 aattgattga tcgtttatac gatgaagaca atggattgcc gaaaaatatc tttgacatcc
    67801 cgtttttcaa agaacagtta aaacaaatga acatgatccc ttgtggctac caccgctatt
    67861 actaccgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa
    67921 gagcgcagca agtgaaagaa acagaagcag agctgtttga attatacaaa gatccagaat
    67981 tggatcataa acctgaacaa ttgcaaaaac gaggtggtgc ttattattca gatgctgcct
    68041 gtgaaacgat tgcttcgatc tatggaaata aagaaacaca gatcgtggta tctacaaaga
    68101 atgatggagc tgttcctgat ctgccggcag attgtgtagt agaagtcact gcctatgttg
    68161 gcgcacaagg cgcacgaaat gtagcgtttg gttcccttcc tgctgctgaa agaggctggt
    68221 tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agccgtgact ggtgattatg
    68281 gaacagcttt acaagcgttt acgatcaatc cgcttatacg atcaggaaag acagcaaaac
    68341 ggatcatgga tgagttgttt attgcacatc agcagtattt accgcaattc aaggaaacaa
    68401 tcagccggtt aaaagcagaa ggaatcgaag tccaagatga actggcaaga gaattatcaa
    68461 gcaatacaca tgtaatggag gaagtatgat gggattcaga aaagattttt tatggggcgg
    68521 agctacagca gccaatcaat gcgagggcgg ttatgatgaa ggcggacgtg ggttagcgaa
    68581 cgtagatgtt gtgccaatcg gagaagaccg ttttcctgta attactggaa aaaagaaaat
    68641 gtttgaattt gatgaagatc atttttatcc agcaaaaatt gcgatcgata tgtatcatca
    68701 ttataaggaa gatattgctt tgtttgcaga aatggggttc aagacttatc ggatgtccat
    68761 tgcttggagt cgtatttttc caatgggcga tgaaaaagag ccaaatgaat ctggtttgaa
    68821 attttatgag gatgtcttta aagaatgtca aaagtacggg atcgagccgc ttgtgacgat
    68881 tacacatttt gattgtccga tgcatttgat caaagaatat ggtgcgtggc gttccagaga
    68941 actcgttggt ttttatgaaa atctgtgccg agtcatcttc aatcgctata aagggcttgt
    69001 caaatattgg ttgacattca atgagatcaa tatgatcctt catgcaccat tcatgggagc
    69061 agggctgtat tttgaagaag gagaaaatga agaacaaatc aaatatcaag cggcacatca
    69121 tgagttggtt gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa
    69181 agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt
    69241 ttgggcttct agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg
    69301 agaatatccc gcttatgcat tgaaagaaat ggaacgaaat ggcattcaga tcgaaatgga
    69361 acctggggat gccgcattgc tgaaagaaca tactgttgat tttatctcct tttcttacta
    69421 ttcttctcga gtttcgacta ctgacaacga actcttggaa cagactgcag gaaatatctt
    69481 tgcttctgtc aaaaatccat atctggaagc aagtgaatgg ggttggcaaa ttgatccatt
    69541 aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccac tatttattgt
    69601 agaaaacggc ttaggtgcag tcgatacgcc cgatgagaat ggttatgtgg aagatgacta
    69661 tcgaatcgac tatctggcag cacatatcca ggcaatgaaa gatgcagtag aattagatgg
    69721 agtcgatcta ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtacagg
    69781 tgagatgaag aaaaggtacg gattcattta tgtcgatcgt gacaacgaag gaaatggcac
    69841 attagcccgt tcgaagaaga aatcattcaa ctggtataaa aaagtaattg attcaaatgg
    69901 agaaaatctt tcgaatgatt aatcaatgat ttataaaagt ctattgtttt tcagcggatt
    69961 tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat
    70021 tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat
    70081 cgttattggc gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt
    70141 atccgtattg atgatcgaac aaggcgcccc tggcggccaa atgaataata cggcagaaat
    70201 cgaaaactat cctggtttcg attcaatcat gggtcctgat ctagcttata agatgtatga
    70261 aggtgtttca agatttggga cagaaaatgc ttatggaatc gtgcaagata tcaaggatca
    70321 cggcagccac aaagaagtta tttgtgaaga taaaacttac gaaggaaaaa cagtcattat
    70381 cgcaacaggc tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg
    70441 tggcgtttct tactgtgcag tttgtgatgg tgcgtttttc cgtaacaaac gtctagtagt
    70501 gatcggagga ggagactcag cagtagaaga agccatctat ttgacacaat ttgcttctga
    70561 agtagtcatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc
    70621 atttgcgaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa
    70681 cgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt
    70741 agcagcagac ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga
    70801 agcaggtttg acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat
    70861 gccaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc
    70921 tgttggtgac ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga
    70981 aacaacagca taagtttaaa aagagtgaaa gaacggtttc aattgttctt ttactctttt
    71041 ttttcgcgtg aaatccactg aatccgattt atgtgaatac ttgcaatcat gcttgataaa
    71101 agaatccttt cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa
    71161 tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca
    71221 agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa
    71281 tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg
    71341 tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt
    71401 aagacaagca acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg
    71461 ccgaggtgtc gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc
    71521 agccaaaaca ctggcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac
    71581 accagagctt tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc
    71641 atctcacaac ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat
    71701 gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa
    71761 agtcgaagta ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga
    71821 agaagtggac gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt
    71881 tgaaacaatg gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat
    71941 gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca
    72001 agctgtggca gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc
    72061 ttttgaatat gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga
    72121 tcctgatgca gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt
    72181 gactgggaac caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag
    72241 aggcacttta ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac
    72301 agcaattgcg gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat
    72361 tgctgaaaaa atccaacaat tcgaagaaaa ccattcacat acttttatgt ttggattcga
    72421 agaaagttat ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt
    72481 gcttttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg
    72541 cttgcaaacg atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat
    72601 gagcggacaa gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc
    72661 accgacagaa tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag
    72721 aacagaccgt gaaggaaacg tagaaaaact cacaacaccg ccatccgacg tattgaaata
    72781 tacattggaa gaaaatggct ggattgctat ccgcccttca ggaactgagc cgaaaatcaa
    72841 gttttatatt ggtgtaaaag gaagttctac agaagacgca gccgaaaaag tagcctctta
    72901 tgaagcgatt atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg
    72961 gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa
    73021 aaatcaaaag tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt
    73081 aaaattctgc tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg
    73141 gaaatggagc agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc
    73201 aagtctcatc gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat
    73261 attttgaacc agacactcaa acatgtggaa catcaataac gataggtgca aatagataaa
    73321 cactggagtg tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag
    73381 acaaaaatga tcaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta
    73441 ggtctaaagc attcaaaata agcgaagccg tttcttcaat agataaagat gctacattga
    73501 tcactacaca tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt
    73561 tttcgatatc ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac
    73621 ggatattatt taaaatatct gggttatttg ttagtcccac gattttttta ggatcgatct
    73681 cccaaagctg tttgggaata tgtgcttgtg ggatcaaagg taaattggca acttttaaat
    73741 ttttgttagc taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa
    73801 cgacatctgc ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt
    73861 ccattgcttt gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc
    73921 gaattggctt gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcaa
    73981 aactaaataa ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga
    74041 ttaatgtatg caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg
    74101 ttttttcatc tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag
    74161 ccatagtagc ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa
    74221 aagtaacgat ttctgtagaa tcggaagtca tggataaaac ctcacttgca ttttttttat
    74281 gtttattata ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gagggataag
    74341 tcatgaaaaa acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta
    74401 aatatactaa aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat
    74461 ctgctaaaga agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga
    74521 tttaccggaa tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg
    74581 gagaaatgaa gtttcgtttt cattgctgtc aggggaaaat cggccatcac catcatttta
    74641 tctgtacagt gtgcggaaca acgaaagaaa tccatatgtg tccgatgaat tatttcgaag
    74701 aacagctagg tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa
    74761 attgtcaata aggatttaaa taaaataaca tttgataatt ttaaaaaaaa gcaagatttc
    74821 cttgacggtc aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata
    74881 tatttcaata acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa
    74941 aacagtcgtt cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt
    75001 gtcaaaagcg ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt
    75061 aagacgcaag aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga
    75121 attggagttg gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg
    75181 aaagcgaaag ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac
    75241 gagcagatta aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgt
    75301 gaaatgaaac agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta
    75361 gctgaaaaag taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc
    75421 gacgaagaaa ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg
    75481 aaagaatttg gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga
    75541 aaccaagtga atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc
    75601 ggacagtgat tatccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa
    75661 gaaagaatct tattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa
    75721 ttgtgtttga tttggttatt cttctggtaa ctcacttgct tcttgtttat atcctgatac
    75781 gcgatataca taataaaaga gcaagctgcc agcgtaacca agcaatgttg ggacgaccca
    75841 gccaagacct aatgcagcaa aaggaacata atgatgataa aatcctagta agcttgctac
    75901 gacagtttgc tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac
    75961 ggcagaaaag aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt
    76021 taaggaaagt aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga
    76081 ccattggatg atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa
    76141 ccgcagccat gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga
    76201 aacaacgagt cccatagcag tagtgaagac acctaatgtg accattaccc ctaagaagct
    76261 agcaccgaag ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc
    76321 tgataaagac atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat
    76381 gacttctaac gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc
    76441 tttaaaacca agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac
    76501 tgcatcaacc gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga
    76561 atctgcagca gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat
    76621 agctaataat gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt
    76681 tggatgtaca gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc
    76741 taatgaatca tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc
    76801 tgtccgcggt gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc
    76861 agcataccat tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac
    76921 tacagctaag atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc
    76981 gctgatccag tttgatccag ccatttgacc taagtgtaca ggaaagatca gattacctga
    77041 cccgaagaac atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt
    77101 ttttgtttct tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg
    77161 ttgcagaaac tagaaatacc actaatatca gattccatga aaaccaccac cttaaaatat
    77221 aaaaaaagcc cttttccact tgggaaaagg gctctagatt ctagaacggt accacctttt
    77281 ttcgcttaaa aaagcttctt cattcacgga cgcttttcga taaagttatc cgctagcctg
    77341 ataatggagg cagccacaat gatctacgtt ttcgacattg actcggaaat gatgttcgtc
    77401 tattcgattt attgtcattt ctcacttaca tgactcactg ggaataaatc actgaatagc
    77461 ctactgtttt cctcgacgct ttgctgtata tttatttgtt gacgttataa tacacctgtt
    77521 ttttttgaaa tgcaagaata tttcaaatat tttgttttct ttttttgaaa caagaaaaat
    77581 acccagagaa tacttgctaa gttagacgga atacttgtcc tctatcttat gcatagctta
    77641 catgatcagc tttttttatt caaacgagac ctttagagaa tggaagcggc tagtttttta
    77701 acgtttcttg gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata
    77761 gtttcttctg agtatggtaa aataggaaag aattaatcga aaaaaagggg atgtctattt
    77821 ttgacggaag agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg
    77881 cttttaggtg cacacgacaa gcatataaaa ctgattgagg aaacaacgga aacaaccatc
    77941 catacacgtg gagaaatcat tcagatcatt ggtgagcaat cccagatttc cttggctgcc
    78001 tcagtcattc ggacattaca ggaattgatc aaacgaggaa tccacgtaag tacgcccgat
    78061 gttgtgacgg cattgaaaat ggggcaaaaa ggtacattag attattttgt tgagatgtat
    78121 gaggaagaga tcgtcaaaga ccgtaatgga aaacctatac gggtaaaaaa cagcggccaa
    78181 aagaaatatg tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg
    78241 acaggaaaaa cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg
    78301 caaaaaatca ttttgacgcg gcctgctgtg gaagcaggtg aaaatctagg ctttcttccc
    78361 ggggatctaa aagaaaaagt ggatccttat ctgcggcctg tttatgatgc tttataccaa
    78421 atttttggat tggatcatac gaaccgattg atggaacgag gcgtaatcga aatcgcacct
    78481 ttagcttaca tgcgggggcg aacattggat gatgcattcg ttatcttgga cgaagcacaa
    78541 aacacgacag tcgctcagat gaaaatgttc ttgacccgtc tcggttatca atcaaaaatg
    78601 attgtcaatg gggatacaag tcagatagac ttgcctcgag ggacgacaag tggtcttgtt
    78661 catgcagaac gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt
    78721 gacgttgttc ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc
    78781 catcaagaat aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac
    78841 tacaagaaaa acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta
    78901 tctgcggcgt tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag
    78961 tagcagaaga aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa
    79021 aaagaaaact ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag
    79081 atgaccagaa caatcggatc aagcaactat ttgaattgat cgataagacg aatgatgcca
    79141 tcaacaaatc ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc
    79201 ctactacaga agagcggatc gccgcgctga agaaaaattt tgaaaatgtg aatgcagaaa
    79261 atgttgtgtt ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg
    79321 aaactgagat tcatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca
    79381 aacaagttcg tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat
    79441 atttgaatgt cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg
    79501 tagtgaatga cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag
    79561 ctgtacaacc agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg
    79621 atgcttcagc tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt
    79681 ttccgctaat tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt
    79741 cacttcaatt ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta
    79801 tgtcagtcag catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt
    79861 atatcccgct gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaatc
    79921 gaagagcagg gatcatggct ggtattttcc aagcagtatc cgcacttttc attttttatg
    79981 attcgattgg cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca
    80041 tggcaacgat tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg
    80101 tcattatttt tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg
    80161 gtgatgcaac gacttggtta atgctcgtct gcgggttcac tggtgcactc ttctcattct
    80221 tgctgacgat cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg
    80281 tattaaatga actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc
    80341 caggtactta tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa
    80401 tcggcggacg ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca
    80461 aacatgctag ctttttcgtt gaaaacttgc cttcaggagc ggaaaacccg cacaatttct
    80521 tattgccaga agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat
    80581 tggaagatta taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca
    80641 cgctaatgcg ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc
    80701 aattccgtta tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg
    80761 acagttgtga ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt
    80821 ttgtccataa tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga
    80881 ccttgaaaga aatccgaaaa gtggaaaaat cattaatcag cggtttaagt agcaccttcc
    80941 attctcggat caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagtacaag
    81001 aagaatagaa agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc
    81061 tcagaagaga aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag
    81121 ctgccagagg aaacagaaat gtctgttact ttcatggaca atgcggcaat tcaagtaatc
    81181 aatcgtgatt accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa
    81241 gaaggcgaag acgaaatccc agtgattttt gaagatggag agaatccatt acctcgtgta
    81301 ctaggagaca tcatgatctc tattgaacgt gctgctgaac aagcagaagc ttacggccat
    81361 tcatatgatc gggaacttgg ctttttagcg gtacatggat ttttgcatat caacgggtat
    81421 gaccatatga caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat
    81481 gcctatggac tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga
    81541 atttgctgtc caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt
    81601 tgcgttcggt atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagattg
    81661 gttatggctg cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt
    81721 tgaaaatgta gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa
    81781 agatatggca gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat
    81841 tttatttgtc cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca
    81901 gaacataaat cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta
    81961 ttgaaccgaa tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga
    82021 aataaaatcc aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca
    82081 ggaatccata aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg
    82141 ttgaaagaag tggatgcagt cttatttatg atcagtgctg atcaaaaacg tggccgggga
    82201 gacgatttta ttattgaacg tctaaaaaac gtgcattctc ctgtttatct gattatcaat
    82261 aaaatcgata agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag
    82321 atgccttttg cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta
    82381 atggatgtat tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg
    82441 actgatcatc ctgaatactt catcgtttct gaattgatcc gtgagaaagt cttgttttta
    82501 acacgagacg aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag
    82561 aatgataaaa tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc
    82621 attatcggca aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc
    82681 gaaaatcttt taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg
    82741 cgagacaaaa aagtctatct gcaagacttc ggctaccgaa aagacgagta ctgatattct
    82801 ggacttctta taggttaggt atagaaataa actggataaa aatccgtacg ttgagaaaaa
    82861 cagcggaaga tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt
    82921 ttttaggagg gagaatatga gccagcttgt agaatcgaaa ggcttgattt tatctagccg
    82981 taattacaaa gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat
    83041 gttttatatc aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt
    83101 taccgaagca gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt
    83161 caaagacgtc catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac
    83221 gtatttgcta aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt
    83281 ccaatttacg caacaagctc tgcagcggat ggaccaagga gatgatgcag aaatcatcac
    83341 gaatatcttt gaagtgcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg
    83401 tgtcgtatgc ggggaaacaa aaggaaaatt tgactattct tccaaatatg gcggtgtgat
    83461 atgcgagaaa cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta
    83521 tttcgttcga atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga
    83581 agaaacgaag caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat
    83641 tcatctaaaa agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac
    83701 gccagagaaa aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac
    83761 aacaaaaaaa tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga
    83821 ttaaatagat cgttcactga gaggatctat tatacaaacc agaaaactgc aaaatgaata
    83881 cgtacattga ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc
    83941 cggacccaaa cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct
    84001 ttattcggaa gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata
    84061 gggacttttt gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa
    84121 ggggcaaaac agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa
    84181 ttttggtcat caaacggctg catgctgatg caagcttatg atacagaaaa aggagcaggt
    84241 acgatgagcc catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat
    84301 gtagagccat cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat
    84361 cagcatcatc aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat
    84421 ttagaaagct tgcgtctttt aggaattgat ccattagaac acgatattcg ctttgttgaa
    84481 gacaactggg agaacccatc gatgggttgt gccggtgtgg gctgggaagt atggcttgat
    84541 gggatggaga tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca
    84601 gttacttctg agatcactta tggactagaa cgtcttgctt cttatatcca agaagttgaa
    84661 agtgtttatg atctggaatg ggctgatggc gtgaaatatg gtgagatctt caaacaacca
    84721 gaatatgaac attctaaata ttcttttgaa gtaagcgacc aagacctgct actaagcaat
    84781 tttgatcgtt ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg
    84841 tatgattata ttttgaaatg tagccataca ttcaatttat tagatgctcg cggtgcagta
    84901 tccgtaacag agcgtgcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc
    84961 aaaatctttg tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca
    85021 aatccagaaa acgtgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg
    85081 gctggaagaa atgcctgctc atgtagtcac accaagcatg aaacaattag aagaaaaaac
    85141 agcaaaattt ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc
    85201 gcgccgctta gcaatcaaag taacaaacat tccagaacgt caagcagaca gcgaagaaga
    85261 agtcaaagga ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc
    85321 tgaaggattt gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa
    85381 aggcgtggaa tatgtctatg taacgaaata cactcacggc aaagaagcaa aagaagtatt
    85441 gacaggttta gcagatgtca ttaaaagcct cactttccca gtaacgatgc actgggcaga
    85501 atacgatttt gaatatattc gccctattca ctggatcgtg gcattgttag atgacgaaat
    85561 aatcccgttt gagatcttgg atgtcaaaac agacagagtt tcaagaggtc atcgtttctt
    85621 aggcgaagat gtgacattta cgcatgcaaa agaatacgaa gaaaaattga atgaacaatt
    85681 tgttattgct tcaccgcaaa aaagaaaaga aatgatcatc acacaagcaa acgaattagc
    85741 agaaaaaaat cactggatca ttgatctaga tgaagattta ctagaagaag tcaacaatct
    85801 agtcgaatat ccaactgtct tttctggcga cttcgaagaa aaatatctat ctgttcctga
    85861 agaagtacta gtcacttcta tgaaagaaca tcaacgttat ttcgaagtac ggaacgatca
    85921 aggtatgttg cttcctcatt ttatttctgc tcgtaatggc gatcgggtaa aattggaaaa
    85981 tgtggtacga ggcaatcaaa aagtattgac tgctcgttta gaagatgcgg aattctttta
    86041 taatgaagac aagaaagctt caatcgacca atacgttgaa aaattaaaaa ctgttacttt
    86101 ccatgaaaaa atcggtagca tctatgagaa aatgcaacgg gtacaagtca tcgctcgcat
    86161 cataggcgaa caagtaggac tatctgagga agaattgatg gatctgcaac gcgctgctga
    86221 aatctataaa tttgatctag taaccaacat ggtaggagaa ttccctgaac tacaagggat
    86281 catgggagaa aaatatgctc tgcttaaagg cgagaaacca gccgtagcaa cagcgatccg
    86341 tgaacattac atgcctatat ccagtgaagg agatttacct gagactacag taggttctgt
    86401 tttagcgatt gctgataaat tagatagtat cttttctttc tttgcagttg ggatgatccc
    86461 aagtggctca aacgatccat atgctttgcg ccgtcaagca tatggtgtcg ttcgaatcat
    86521 cgaatcgaaa aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgaagttat
    86581 cagtaaagat acggatcgtt ttggcattgg cttgtcagcg ggacaacaac aggtgatcga
    86641 ctttatcaaa ggccgtctgc gccaattatt gacaacgaag aatatccgtc atgatgtcat
    86701 cgaagctgtt ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt
    86761 gttcaaacaa catcttgcag atgaagactt caaaccttca atggaagcat tgacacgtgt
    86821 cgtgaactta gcgaaaaaag cgcagcttga aaaacaatca gaagtagacc ctgagctgtt
    86881 cgaaaacgac gcagagaaag aattacacaa agcagtagaa gcaatgaaag caagttttgc
    86941 tgaccaaacg atccacgaaa actacgacca gcttgtcgct cttcgtccac tgatcgaaaa
    87001 ttattttgat caaacgatgg tcatggctga tgaaccagca gtacgtaaga accgactaac
    87061 acaacttagt caaataactg ccatggcttt gtcattagca agcttggatc aaatcatcac
    87121 aaaatagtct tttgtttgaa aaacagagag catgcacgaa acgtgctgtg tctctgtttt
    87181 tttgttataa atataaaagc tgttattaat tctattttaa taatttttgt tattttattc
    87241 tttgctagaa ttttcaaaac gctcatgaaa agtgaggaaa taaaaatgaa aaagattaat
    87301 aataatcttg ttactgtttt atttactttt aaattaggaa tttccctagt caagaaaact
    87361 gtttgtggta tgattatata gggaaaacct gtttataaat atttctatga atatattagt
    87421 ttaattatgc ttttaaaata aaaagcgctc attctgatgt gaatgaatca catctttatt
    87481 tatctatcat caatatttag gagtgaatct atggaaaagc cgttcgtcaa gttattggct
    87541 actgtggcgg taggcacagg cgctattgtg atttgcttgt ttggatacca tctcaataat
    87601 cagcggcaac atcatcaacg gatcaattac gcagaatctg ccatcaccaa tcaaaaagac
    87661 accgtgacct ctttatcaaa agaagtagat aagctgtaca gtacaaaaga aaaaattttc
    87721 ctaaatcctg aaataacaga agaaacagtt tcaaatttgt cacataaact atcttctatc
    87781 aaactttcag cagatgatta tgacatcaag gaatcagaat tacctaaaga agctgctgcg
    87841 atccaagaag aaaagaaagc agtcttaaca cagttagagg atgcagaatc aaaactgaag
    87901 atccaaacgg ctgtcaataa attatttact aaaaacgtat caaactggca gcaagctgtg
    87961 gatgatgtca tcatcaaaga aaagttggca agtgcagatg tcgcccatgt acgtgaaaat
    88021 atgagctttt ttaaggatag tgcttggaaa acggtcgtta tgcaatatct aggattcgct
    88081 gatacccaaa ttgcacaagt cacgcaacta gatcagctat tcgatacgat gctgaaagac
    88141 ggtcaagtaa cagctactgc tacttacgat caatatttga ccgcactcag tctcatcgaa
    88201 cagatccgga atgaaaaaat aagagccgca tacgcgtcaa aagcagaaac agtcgctcag
    88261 caaatgggct atagcaatac gagctattaa taagcgagaa ttcaagcaag gctagcaaac
    88321 ggaggaaact atggcaaaac gaagaagaag atcaagaggg aaaaagatat ttctaattat
    88381 tttagcaatt cttgtcgtat tagtattagc aatcgtagga gtaggagcaa aattatatct
    88441 agatctctca cattctattg aaaaaactta cgaatcagtg gaacgaaccc aaggagaacg
    88501 aacaaaacca gttgatctgg acaaacaaga atctttctct gttctattga tggggattga
    88561 tacgggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg ttgccacagt
    88621 cagtcctgaa gacaaacaga caacgattgt cagcattcct cgtgatacgt atgtcgacat
    88681 cgttggacat gacacaaaag acaagatcaa ccacgcttac gcattcggcg gagctgccat
    88741 ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg tttcaatcaa
    88801 tatgaaaggt ctaaaagaac tcgttgatgc agttggtggt atcgaagtca ataatgacct
    88861 caccttctca caagatggct atgattttac gatcggtaaa atcacattga acggggatca
    88921 ggcattgtct tattctcgta tgcgttatga agatccaaat ggtgactacg gcagacaaga
    88981 acgtcaaaga aagatcgtag aaggtgtagc gaaaaaagtg ttgagtctga acggtgtaag
    89041 caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca tgagctttga
    89101 tgaaatgaaa aagattgctt tagattatcg ggatgcattt ggtacgatca agcaagatca
    89161 gctgcaagga gaaggcttca tgcaagacaa tatctcatac caaaaagtca gcgatcaaga
    89221 acttgatcgt gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata aataggaaag
    89281 tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat tacgcaaaca
    89341 cgtgataaca atcctcatct caatgtttgc tggcttggcc atctcaggca tcgtgacgtt
    89401 tttcatcttg acgccaaaat acagctcaca agcccaattg atcgtgacac tgccgcaatc
    89461 agatactgca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata cgtataagga
    89521 tatgatcgtc agtgatctag tactaaatga agtcaaagat cgtttagaaa cagaagacaa
    89581 tgtaaaaatg actgctggac aaatcaaaga tgccatctcc gtcaatcagt cacaaaattc
    89641 acaaatgttc tcgatccaag caacaagcac aaatgcagtg acggctcaac aaatcgctaa
    89701 tacaacgtca caagtattcc aagagaacgc caaagacgtg atgaatgtcg ataaaatctc
    89761 gatcatctca ggtgcagttg ccaatacgac acctgtttcg ccaaataata aattgaatct
    89821 agtgattggc ttagtgttag gaatgatggt aggcgtaggt ctagctttct tatttgaatt
    89881 actcgaccgg acagtaaaag atgaaaaata tgtgacggat acattgggct tcccgatctt
    89941 agggactgta ccagaaatgt cagcgaaaga attgaatgct ggtatccgta aaaaaggacc
    90001 agctccgaca caaccaaaag ctacaaacga taaaacagcg ccatcacgca gaagccgctc
    90061 aagagtctag gaggaaagaa aaatggcacg aacacagaaa caaaaagcag cccaaactca
    90121 tgcagtcagt ttggtcacac tcacaaaccc ctcttcacca atcgctgaac aatatcgtac
    90181 gattcggacg aatatccagt ttgcctccgc agctggtcag caaattaaaa cgattgttgt
    90241 gacttcatca ggacctggag aagggaaatc gaccactgca gcaaacattg ccgttgtttt
    90301 tgccaaatct ggtcaacggg tgctgctagt cgatgcagac ttacgtaagc ccgtcatcta
    90361 taaaacgttc aagttgaaca acgcaagcgg attaagtacc gttcttagtt catcaggaag
    90421 tgtagcagat gagatccaac gaacaccagt cgagaatttg tcgattctgc caagtggacc
    90481 taaaccacca aatccatcag aactactaag ttcgccaaga atggatcaga ttttagcaga
    90541 agcaagacaa ctatttgatg tagtgatctt cgacatgccg ccagtagttg ccgtaacaga
    90601 tgcacagatc atgtcttcca aaacggatgg caccttattg gtcgtacgtg aaaatatgtc
    90661 acggaaagaa tcattgacta aagcaaaaga attgctggat atggtacaag cgcgagtcct
    90721 tggtgttgta tacaatgggg cagaacacag caaagatgct ggctattact acgggaatta
    90781 agaaataaaa agagaggaga caagagaaat gatcgatcta cattgccata tcctgccagg
    90841 aatcgatgac ggagcagaaa acatagatgc cagtatcgcc atggctgaaa aagcgattca
    90901 acaagggatc acacatattt tatgcacacc ccatcataat aatggaaaat acagcaatga
    90961 aaaatcacaa gtcatttctc tagtcgcttc gttacaagca gaattagaga aaagacaatt
    91021 gcctttgacg ttattggaag gacaagaagt acgtattacc ggaacactga tcgaagacat
    91081 cgaccgagat gaaattttat ttacagattt agatgataca tatcttctaa tcgaatttcc
    91141 gacactggaa gtgccgttat atgcagaaca gctctttttt gagttgttac aactaggaaa
    91201 gacaccagtg atcgtccatc cagaacgcaa cgcgcatttt cgtaaagacc cgaatcatat
    91261 gctgccattc ctagatatgg gatgcttggc ccaactcacc gcaccaagtt atgtcggtgt
    91321 atttggaaaa gacatccaga aaacagccaa aatgatggtc aaacataatc ttgtgcagat
    91381 ggctgcttct gatgcccatg gggtctctaa gcgtaccttt tacttaaaag agtgctatga
    91441 gcagattgct aaggactttg gtaaggaaaa agtagtacag atgaaacaag tcgcaaagga
    91501 tctgatcaac ggggatgaaa tcagttatcc agtctatgaa gaggtaaaaa agaagaagtt
    91561 tggtttattt tagttcttta ttcgtagata aaaaggaaat gaggagagag ggaaagtatg
    91621 gaaagtgaag atttattatc tcgtattagt gaagtacatc ctaatcaagt ttggttggat
    91681 aaagaaaaag ttgaaaaacg atatatctat ttatttttta aaagaatgat tgatattgta
    91741 ggaagcatta ttggtttgat tttagcttct cctttgatgt tctatgtagc ttatcgaatc
    91801 agaaaagaag agccaggaag tccgatattt ttttgccata cacgtgttgg taaaaattga
    91861 aaaacattca aaatgtataa attccgttcg atgtgtttag atgctgaaga aaaattggaa
    91921 gaattacttg atcaaaatga aatagaaggt gcaatgttca agatgaaaga agatccccgt
    91981 gtcactaaaa taggtaaaat tatccgtgcc aaaagcattg atgaattgcc acagctatgg
    92041 aatgttttaa aaggcgatat gagtctaatt ggtccacggc cacctttgga gagggaagta
    92101 gctgagtaca cgagatatga tcttcaacgc ttgtatgtaa aacctggttg ttcaggtttg
    92161 tggcaagtta gtgatagaaa taatgttagt ttccataaaa tggttgagtt ggatttagag
    92221 tatattagtc atcgaagttt aatgtacgac ttgaggttga ttttaaaaac gttttatgtt
    92281 attgtcaagc ccaatggagc atattagtta gggatagaat gatggattgt taataaaaaa
    92341 gattacacat gttcatatta tcgtttcaaa agtaataact gtaattatgg agggtttgaa
    92401 tcacttgtag aaaatctaaa aaaagaaagg tgaatggggc aattcagtat cattattctt
    92461 atataaaaga aaatattcga aattttggga tgataccact cttaaatata atgatctaaa
    92521 tgctttaata ttggtatatc cgatatagga actgacgagc tatttagtat gatacaaaag
    92581 tatattgata gccattaata aattttccaa aaaataatga cagcacacca atttttataa
    92641 tttagcttgt tgaatcggta tgttcatggg acggttcaaa aaaagaatcg aaaaataaac
    92701 ggtaaacttt tagtgaatcc taacggatat gagtggttac gagcaaaagg gagttatccg
    92761 gtcccaaaat attgtaatta ttctgaaaca ttaatggaat ggtatcaact gataataaca
    92821 taataagaga taaaattgag gaatatctat cattttataa caaagagaat gctgcagagt
    92881 gtttacttaa aatgtgggag taaggtattt gtgactttag aagaggctat tttttatcta
    92941 taagaattta ttatccactg tgcagaaatg aagattatag ttcatctaaa cttttaaact
    93001 ttgtaatata ttggatgttc ataacggaaa ataatatttg gaaaagtacg ttgttataaa
    93061 gtatgtacca ctatcttaaa tattatgaaa ctaagacaat tttttttgaa actatctata
    93121 gatatattta ctggaatttt tggaggtata gaaacagtta tcattttatc tttgtaccac
    93181 caatgattta tatcagctca acagagtgaa ttgtatttac gcaagaatgc gtaggatgaa
    93241 atggatagat aattaagata tatatgtata tggtgactga agacaaaaaa acagaactaa
    93301 tgaaactgaa taaaaatata gagtgaacta gtttctaaga gggattttaa actagatttc
    93361 attggtaata aaatttggac agatggaaaa acatcacata aggaagcaga ttttgttgtc
    93421 atttttgttc ctacaaacta tggtagtgaa tgtgttattg aattagtgat gtctgttact
    93481 tcagaggcat tggggataat taaatccact atacctgtag gatatacaaa aagtggaaga
    93541 aaaaaatatt atcaatagat tttatttagt tcagaatttt tacgtgaaag tagattatta
    93601 atgataatct ttatccaatt agaattatta tcgtaggatg tgatgatgaa agccgtgaaa
    93661 ttgttcaagt atttaccaat gtctccctgc cagtaaatcc gcttagaatt tgagccttga
    93721 aaatgtgcaa tgataaaaaa caaaaactcg ctcaaaatag acagtttggg gtgggaatag
    93781 accaataatg taccaaacaa acttccattt ggggtgaaaa taggtcaata aagcgatgtt
    93841 aaacatacct ttggagtaag aatagagggt aataagtgaa tttataaact ttttggtgac
    93901 tggttgagtg gaaaatgtag gtacatttca atgcgaaaat gtattataac cccaaacgag
    93961 gtcaataaaa atgactcaaa cttgcaatat aaccctaaac gggtagggga tttatgaaaa
    94021 attgaatttg ccggcgtagg agctggctgt tttaagtatg aatgaacaca gctttcgggc
    94081 tgttttatat aaatagcagg ttactgttca caaacagcat tttgttaaga aagtagtgac
    94141 cacagcagtg gtggaaagag gaaatattat gaagaaacag tataacattg cagtcgccgg
    94201 caccggttat gttggtttga gtattgccac tctgttggca cagcaccata aggttacagc
    94261 ggtggatatc attccagaga aggttgaact gattaataac cgtaaatctc caatccagga
    94321 tgattatatt gaaaagtact tggctgagaa agaactgaat ctgaccgcaa cattggatgc
    94381 aaaggcagca taccagtctg cggattttgt tgttattgcg gctcctacta actacaatag
    94441 ccgcactcaa cattttgaca catccgctgt tgaggctgtt attaggctgg tcatggaagt
    94501 aaatcctgat gccattatgg ttattaagtc caccattcct gttggataca ccgcttctgt
    94561 tcgtgtaaag actggtagca agaatattat tttcagccct gagttcctgc gtgaaagtaa
    94621 ggcactgtat gacaatctgt atccttcgcg tattattgtg ggtaccgata tggatgatga
    94681 gcgtttggtt gaggatgcac acacttttgc tacactgcta caggaaggcg caatcaaaga
    94741 gaatgtggac acattgttca tgggctttac tgaagcagaa gctgttaagc tgtttgccaa
    94801 tacatatctg gctcttcgtg tcagctactt caatgagctg gacacttatg cagagatgaa
    94861 aggcttgaac actcagcaga tcattaatgg agtttgcctt gatcctcgta ttggtactca
    94921 ttacaacaac ccttcctttg gttacggtgg ctattgcctg ccgaaggata ccaagcagct
    94981 tctggccaac tacgcagatg ttcctgaaaa tctgatcgag gcaatcgttg aatctaaccg
    95041 tactcgtaag gactttattg cggatcgtgt acttcagatg gctggttatt acaattacgc
    95101 agaagcaaat cagtatgatg ctggcaaaga aaagcctgtt gtgattggtg tttaccgttt
    95161 gaccatgaaa tccaattctg ataacttccg ccagagttct atccagggcg ttatgaagcg
    95221 tgtaaaagct aaaggtgcaa ctgttgtgat ttatgagccg accttgaaag atggagagac
    95281 tttctttggc agtaaggttg tgaatgattt ggaacagttt aaggcaatgt gtgattctgt
    95341 tatagcaaat cgctacgatg cttgccttga tgacattaag gaaaaagtat atacccgtga
    95401 tatcttccag agagactaag attaggagag agtatttatg attattacaa aaacaccgtt
    95461 tcgtatgtct ttttttggcg gtggaacgga tatggaggat tatttcaaag agaatggcgg
    95521 tgcagttctt tctactactt ttgataagta ctgttatgtg aatgtgagac atctgcctag
    95581 attttttgat tacagcgctg agttatctta ttctcgtact gagagagtaa ctagcattga
    95641 agatattgag catcctgcga ttcgtaatgc gatgaaaatg ctcgatatgc atgaaattcg
    95701 gcttacatac gaagctgatt tacctgctcg ctctggtctg ggtacatcca gttcttttgc
    95761 agttggtatg ttgaatgctt tttatgcact ggagggtaag tatgctgaca agaagaaact
    95821 tgctgatgaa gccatttatc ttgaaagagt tctgtgtaat gaagccggtg gatggcagga
    95881 tcagattgcc gcatcttatg gtgggttcaa tcgaattaat ttcaatgtgg atggttatga
    95941 ggtgctgcct gtcattattt cgcctgaacg taaaaagcag cttaacaaca atctgatgat
    96001 gttctttacc ggattcacta gattttcttc tgatgttcaa aaggctaaca acgtcagtgg
    96061 cacagaggat aagagagtaa gactgaaaaa gatgtatgag cttgtagatg aagctgaggc
    96121 aattcttact gataagaaca gaaatttaga tgatttcggt agacagcttg atgttacttg
    96181 gagattgaaa aaaggtactg gtggagcaat tagtacagga agcattgatg aactatatga
    96241 aaagggtatg gctgcaggtg ctttaggcgg aaagttgctc ggtgcaggtg gtggaggttt
    96301 cttggttttc tatgtacaac ctgagaaaca ggatgcagtt cgttgggcta tgcgtgattt
    96361 gatgtatatt ccgtttgaat ttgaaaatgg tggaactaga gttattcact acacgccgga
    96421 aacttatatt gaaaaacaga atggataagg agtaacaatg ttagcggaaa aacttaataa
    96481 acatattgac ttattgatta aacgatatcc tacacttgag acgattaaac aggacattat
    96541 cgatggatac ctaattatgg aggaatgcta tttgaatggt ggtaaacttc ttgtggcagg
    96601 aaacggagga tcagccgctg actcggaaca tattgctggc gagttgatga agcggtttaa
    96661 gacgccacgt cctgtaagta aggaatttgc cgataagtta gttgcaattg accctgtaag
    96721 aggtccggaa ttagccaaaa atctcgagtg tggtttaacg gcaattcctt tggtcgcaca
    96781 cgaagcattg tctactgctt atattaatga tgtcgatggt ttgggtgtct tcgcacagca
    96841 gctgtttggc tttggtagat ctggtgacgt attccttggt atttcgacgt ccggaaatag
    96901 caagaatatt atgagcgcaa ctgttgttgc tagggctctc ggtataaaag ttattggctt
    96961 gacgggcgca aagggtgggg aacttgccac ggtagcagat gtttgtgtta aggctcctga
    97021 agttgaaaca tacatgattc aagagtatca cttgccgata tatcattgtt ggtgtttgat
    97081 gctggaagat aaattctttg gaaacgaata aaatttggag gaatgttaaa tgaaaaagac
    97141 aattatggtt accggtggtt gcggtatgat tggctccaat cttgttaaga gactggtgaa
    97201 ggaaggctgc tgggatgttt atgtagctga taatctgtgg agaggaaagt tggagtacct
    97261 gaacgacgaa gatgggcatc ctgtcatcga cctggatact catttcttca atgctgatct
    97321 gactgattat gagcaggcta aaaaggttat tggtacgact gagtatgtag ttcatctggc
    97381 tgatgttgtg gctggcattg actatgtgtt taagaaccag ggcgaattgt tccgaatcaa
    97441 taatctgatc aattctaatg tttttgattg ttgccgtaag gctggcaagg agaaaatcaa
    97501 gggtgttatc tatgttggta ccgtatgctc cttccctctg accagacaga atactttgaa
    97561 tcctgagcct ctccgtgaag aggagttgtt ccctgctctt cctgagtctg cttatggctg
    97621 gagcaaactg atgggccagt tggagatggg gtatttggaa aaagaaaccg gaattccgtg
    97681 ctgtatcctg atgctccata atgtttatgg cacacctact gattttggcg aacgtagtca
    97741 ggttattcct gctctgattc gtaaagcaat caataatcct gaagagcctt ttaatgtgtg
    97801 gggtagcgga gagcagggac gtgctttcat tcatgtcaat gatatcgtaa atgcattggt
    97861 gctcgccctg gataagggtt ggggtcatgg tcatattcag attggccctt ctcactgtac
    97921 ttccatcaag gaaattgcat ataagatcat tgaaatttct ggcaaagaca tcaagccttt
    97981 ctttgatact tctaagcctg aaggtgataa ggctcgttgc gcagattatt ctaaggcgaa
    98041 agagattttg gattgggagc ctactgtttc tttggaagag ggtcttcgtg aatcctacaa
    98101 ttggattgaa aagcagatta agggttaata agtatataag ctgttcttcc tccgtgtaat
    98161 tagaggatgc ggaggaagaa catatgagta aggatgtact gagatggaaa agaatatgta
    98221 taaaagaaaa cgtgatttca ctgtgtgtgg aatagtaggt gtttgtgcta ctacaaagaa
    98281 cgagtttgta agggaagttt tttccatgcc aacatctccg gtaagtgcga ctgttgatct
    98341 cgtgggagtg ccggccatta tttcggcaaa ggaaaatccc aaaattgctg aaatgtatag
    98401 ccaggctaca atggctgcaa tagatggtat gcctattgtt aaaattggac ggcgcaaagg
    98461 atttacctgt gaacgatgtg ctgcaccaga tattatggga atggtttttg aagaaggcgt
    98521 tagacaggga aaaacacatt atttctatgg tggaaaagat aacgaagtgt taaataagct
    98581 tcgtgcaaac ctagaaagag attacccggg aatcaaaatt gttggaatgt attccccacc
    98641 atttcgtccg ttaaatgaag aagaggatag caagatttgc gaggaaatca acaacttaaa
    98701 tccagatttt ctgtgggttg gtattggtgc gcctaagcaa gaaatgtgga tgtgggaaca
    98761 ccagaagaag atccataaca cagtaatgtt cggtgtaggc gccggcttta acttttttgc
    98821 gggcactttg gaaaaagctc ctgaatggat ggaaaaagct agtctagagt ggttatttag
    98881 actggtaaag gaacctcgta gactatggag gcggtatatc ttgggaggta taaagtatat
    98941 ctactatact attgaatcta agtagggggg caataaaaat agatgaaaat acttatagta
    99001 gttacaatga cgaataactt tggtaagaag gggtcctata attctcagga aataggatta
    99061 gctcgagggt tagctgaatt aggccacgaa gttattgtat ataaaagtgt aaaaagtgat
    99121 gaggaagttg gaacagagca attaagcaat acggcgatga ttcactatat caagtgtcgt
    99181 tatgtaggat caaatggatt cttatcaaca aagaattttg ataagaatgc ggatgttcta
    99241 atacaatttg cggaccttca actatcagta cctcatgtat actcatggac tgtaaaaaat
    99301 ggaataaaat atatacctta taatgggata acgcgaagtc aaagtggcaa taaatatata
    99361 aagacaatta ttgaaatatt ggcgcatcga aattataaaa tttataagaa aaatgggtgt
    99421 gttgccaaaa atgatcgagt ttctgaaaaa ttagtacaat taggagtaaa taaagtgaaa
    99481 cttgctcctg tttgtgtgga ttttgaacag ttacataaga attacgaagc aatagatttt
    99541 actactctca gagaaaaatg gaatattcct cgcaatgttc gtgttatttt atatgtagga
    99601 cggtttgaaa aggataaaag accgttagat atgattagtt tatttaataa acttgatgag
    99661 cataattatt tgctgatggt aggaactggt gctttactcg agcaaacgaa gcagcagata
    99721 aatttacttg gactgcagaa tagagtaaaa ataattgagc gtattcctaa caaagatatt
    99781 tgggagttat attgcttatc ggatgtattt gtaaatctaa actatactga aatttggggt
    99841 atggttttgc tagaggcaat gtactatcgc ctcgggatta ttgctgttaa tgcacctggc
    99901 ccacagaata ttattttgga taatagatat ggaatgttgg ctaaatctga cgatgaaatt
    99961 ttgacattct taaaagacgg acgatttacc aacgatatca tagatgcagc atatcagcgt
   100021 gtaactacag aatttagatg gtcaaagtcg ggagaaatta tgcttagcat gttggaaggt
   100081 gataaatgat atggaagtaa cgaaattaat ggcattgagg aatcgatatg ctttaaacat
   100141 agttgataat tgcacgagaa aaatagccaa aattcttggt tgttgtattg gtaaaggtgc
   100201 acagataggg aactctgttg aatttgttca taattctgta ggcactgtga ttcattctga
   100261 cacaatcctt gaagatggtg ttaaagtcta tcagaatgtg acgtgtggtt gagcagatag
   100321 taatgaacgt atcatcattc gagtaaaaaa gaacgctgtt ttatgtgtgg gtgctaaaat
   100381 tctttgtaaa tctaacacag aattatgcgt tggggaaggt gctgtagttg ctgcaaatac
   100441 agtggtattg aggtctattc caccctacga agtgtggggg gtacctgcta aaaagatggg
   100501 gagataaaac atgaatgcat ataaattata caaagtagaa cgatggtttt acttacacaa
   100561 aatggagttt tttgccaaga ttgtaaaggc actaatttat gtacttcata atagctcaat
   100621 ttcatatcaa acagaaattg gagataattg taagttctta tacggtggca taggttgtgt
   100681 aattggaaaa gatacaaaaa taggtaatca cgttataatc ggaacgaatg tactgactgg
   100741 tggtagatcc aataagcctg gtatgcctac aattggaaac aatgtatata tcgctactgg
   100801 cgcaaaaatt ttaggtaata tcacaatagg ggacaatgta atagtagggg ctaatgctgt
   100861 ggtcattaat gatgttgaac ctaattgttc ggtaggaggc gttccggcta gagtattgaa
   100921 aaaagatatt gatgtaaatg attattgtag tttgaatcgt tactaaaagg agagattgta
   100981 tgttgtattt tgttattcct ttaatgagtg cgaaggcagc aaacaattgg gagatggtta
   101041 gtaccttatt taaccgaaca ttatggtcgt gttataatca gactgatcct gatttcaaaa
   101101 tcattgttgc ttgccatgaa attcctaaac tcacgcatga atatgatgac agagttgaat
   101161 ttattcaagt tagcgaaaag gatgccccta ttcccacaaa tcaagacgaa aaaatgattg
   101221 ataagggtta taaaacacat gctctcgcaa tgcgtttgcg agagttgggc ggcggtttcg
   101281 ctatgatggt tgacgcagat gatcttgtaa gttctcattt ggcggaatat gtgaattccc
   101341 atccccagga aaatggattt tatgtcaaaa cgggatacgt gtactttgtt ggagacgatt
   101401 atatgaaggt tctaccaaag ttctcatcag gaagtgcgtg tatagtaaaa tatgcagtgg
   101461 aagacctgcc tgaggcatat ccggaagtta tgacagcaaa ttgtgatgat aatccatgga
   101521 tcattagaaa acgacatggg ggcgttgttc cagcatgtga agagcaaggg cgtccattaa
   101581 aacctcttcc attcaaagca gcagtatatg tattaggaac aggggacaat cactcgcttt
   101641 atggtaaaac gacaaaatat cagacgaaaa tgcgagagat actggaaatg tttatagcaa
   101701 aacgtaaaat taaaggaaaa ctgaaagcag aattttctgt tgattggtta taaggtgagt
   101761 attatgaagg tattgatgtg tgaaagccaa atgccagaga attggtattc gtttgatctt
   101821 tctaaagaac ttaatcaata tgttgattta acattgctgt gtaagcgtga tgttcctcct
   101881 ttagatccta aaattaagag caaacatatt ttgtatcata aaagtacaaa taaagtgttt
   101941 tcggcacttg tttttctgaa aagcttgttc ttaatttgga aggaaataag atttggtgat
   102001 tatgatattg ttcatttgca attgatgaaa agtctcaaat atgaaggcgg attgtacgtt
   102061 ttatgtaaga gacactataa aaaattaatt atgacagttc ataatgtgtt accacatgag
   102121 gttcgtgaaa aggatcgcca agagcacttt aatatatatt caaattgtga tggtttcatt
   102181 gttcacaatc gagtaacaga acagcattta tatgctgaat tcccagaatt aaatgcaaag
   102241 tcatatgttt gtccacatgc tatctatgga gatgttaaac atatatctag agcgtatgat
   102301 aaagataagt ttgaatttat cttgtttgga caaattcgtc catataaggg aattgatgta
   102361 ctgcttaatg caatcacaga acttccaatg gatgtaagaa agaaagttca ttttaccata
   102421 gcaggtcaac aacatcctaa acaggatagt actgactata aaaaaatgat tgaagattta
   102481 aaggtttcag attgtgttct atataagcca gagagaattt ctgatgaaga aatggacagt
   102541 atgttcagaa atgcagatgc cgcaatattt ccatataaag aaatctatgg aagtggagcg
   102601 cttcttatgg catacactta ttgcttacca actattgtta gtaatgtacc tgcttttatg
   102661 gaagaaaccg aggaaggaaa gatagggtta cttttcgaaa atttgaattc taagagccta
   102721 gcagacacaa ttgtaaagta tgtgaacctt agcccagctg agaaaagcaa aatagaggat
   102781 gatatatatg agcttgtaaa ccgcaagtat acctgggcgg catctgcaaa actcacatac
   102841 gaggcataca aacaaatttt gaatgagtag taaaacaatt agaaaggaat aataattaac
   102901 atgcagatta gtaaatggaa aatcatacag tttctaatgg tctttctaat gattatctca
   102961 tcaagcatat tgaagccttt gggcggctgg ttgggagaaa cgatgaacgt tagacttgtg
   103021 acagtcattc tagcaatagt atgtttgtta atagcctcat taccagcatt tagaaaaaat
   103081 agatatttaa atttctgcac tttactgatt tgtgttgtta taatagcagc ttacatctac
   103141 tcagataaga caatatgggt taaatctagg gaaagtatga gtttatacta tgtattattg
   103201 gcgtatccat tgttctgtac tttaattaac caagcgtgga atttggattc aatgctaaat
   103261 acgatatgtg gtgtaacatt tctttcatac attcttagaa catctatttc acttattcag
   103321 aaattttcag gagtacttct ctatgaaaac atatatcgtg agtgtgcccc ggaaaattgg
   103381 tatcgtggag gctttttaag aattaatcca ccatgtttta caattataat aataccgatt
   103441 gctttgtata tgattgagcg acaagttgta gctagaagaa aagtaaaata ttatttactg
   103501 attgctagcg cactggcata ttctgctgtt attcatggtt ctagatctgt ccttgtctat
   103561 caaattattg tacttggttg ctatatcctt tttaagaaag gttcttcgaa aagaaaaatt
   103621 gcaatgtggg ttttagctgg gttgcttgta gtcatattta ttaattcgac catgttctca
   103681 acatttgttg aaactttcac aaacagtaca ggtgaatatg caggatctgc tgaatctcga
   103741 tttgcttcag tttggttttt tattcctaaa tttttgcaat cgcctttgtt cggtactggt
   103801 attcttactg gtgaggaatt tacgtttata ttaccaggag gagttagagg acacctatct
   103861 gatattggat ttttctatac aattactcaa tatggtgtag gtatacttat atttgatatt
   103921 ctgtttatta taaaagggtt caaaacagca ttacttgcat caaaagtggg tatgactggc
   103981 tatcaatatt tagcatttgg aattacatta aatgtcttgc taacagggat taatatagac
   104041 tggttttatc cgatatttac tccagctatt cctgtatgct tagcggttat agaatacatc
   104101 tatcaagaat gtagaagtgt ggctaacata gaataggaaa tattaatagg agtgcatctc
   104161 atgaataaga aagagatttt atataacata ttgaagaagt acgaaaattt accatatgtg
   104221 tttaggaaca tgtttggtca taatataagt aaactcgtat cttggaaatt gactgctgat
   104281 tgtgcaacag tagaagaaaa ttctgaagtt tatgcagcgt ttcaattgcg ctcaaaggac
   104341 atgtctgatg ttccagtgat gggtttagtt aattgttgca aagatgtagc aattgtaatg
   104401 caggggccac tatgtttaaa ggatgatttc tcttataata ctctggccta ctataagaaa
   104461 tgttatccaa atgctttaat tattctttcg acatggaagg acgaagatat taaggcattg
   104521 aaaaggatag aggatcttgg agttatagtt atttgtagtg agaaacctga aaagcctgga
   104581 cacttaaata ttaattatca agttggaaac actttgcctg gaatagtcag agcaaagcag
   104641 cttggtgcaa agtatgtttg taaaactaga acagatcaaa gaatatacca tccaaatgca
   104701 atggcgtttt tctgcagttt acttgaacag tatcctgtta ataacgaaga tgattggaaa
   104761 ttaatacaaa atcagcgatt actgttacta tcaatgccgt atggagacat gtttttcccg
   104821 tattgtttgt cagacttcct gtattttggt gatgttgatg atatgataaa cctcttctcg
   104881 attcctagtg atgtgagaga aaagggagct gtgtctagag gagtctcaag aagggagata
   104941 tctgaaaata atttagctcc agaagttcag cttcttcgaa gctatatttc acgaatgggg
   105001 ggcaatgagg agtgctctat tagggcatat tgggagtttg taaaaaacca tgttattact
   105061 attaacaaaa atcaaattga tttgtactgg cataaatatg taggtcgtta ttcaaataat
   105121 actatttatg gtacgtatta tattgatgat agccaagatg cacttcactg ctataatttt
   105181 gattttataa attggttgaa tttgtacact ggaaaatacg aatacaaagc agaatatgaa
   105241 aaatatatgg actttgtttg ggaagagtaa ttaactttta tagtaaaaag tactttaggg
   105301 aagggcatat tatgaaaaca gtaattatgg cgggcggacg aggaagccga atttcggaat
   105361 tgtttccgga tattcctaag ccaatgattc ctatagatgg cattccagtt ttagaacgag
   105421 aattgtgttc gcttagagat caaggcttta atgatattat tttgactgtt tcctatttgc
   105481 atgaaaaaat agaggagcat ttcggagatg gttcaaaatt aggaattaaa atcaaatatt
   105541 ttattgaaaa aactccactt ggcaatgcag gtgcattgta ttttttgttg gatgaactgg
   105601 gtgatgaacc gtttttactt ttgaatgctg atgcagtatt tgatgttgat ttcaggcaga
   105661 tgattagatt tcatgttgag aataacgcag atgttacttt gtttacgcat cctaactcac
   105721 atccgtatga tagtggatta attatatccg atgacaataa gaaagtgctt caatggttag
   105781 cgaaggaaga tcaaagacct gaatattata aaaaccgagt aaatgcaggt cttcatgtta
   105841 taaatccgaa ggtgttgaga gaaaatccat taatggaaat tgtaaataaa tcaatagttg
   105901 aaggtggggg gctaccatac aaaattgatt tagatagaat gattctgaaa ccattggcta
   105961 acacagggcg tatgtattgc tacgattctc cagagtatgt taaagatatg ggaacaccgg
   106021 accgatatag agctgtaatt aaggactttc aaaagggaat tattgttggt aaaaatctat
   106081 ccaatccaca aaaagcaatc tttttagata gagatggaac gattaataaa tacgttggtt
   106141 ttttgagaaa tgcagaagaa tttgagttaa ttgatggagt atcggaggca attaaaatta
   106201 ttaattctct aggttttctg tgtattgtta taacaaatca gcctgtaatt gcaagaggtg
   106261 aagttactgt tgatgagctt aatagtattc acaataagct tgaaacaatg ctagggcatg
   106321 ctggtggatt tttaaacggt atttattact gtccgcacca tccccataaa ggatatgcag
   106381 gagaggttat agaattaaaa attgattgca attgtcgcaa gccaaaacct ggactgttat
   106441 tagaagcatc aaaggatttt aacataaacc taagtcaatc atttatggtt ggagatagtg
   106501 aaagtgatgt aattgctgga aaaaatgctg gttgtaagac agtactgatt acagaagaca
   106561 tcaccttgcc tgctaattat ggccaagact atagtgtctg ttcattgtta gaatttgtta
   106621 aaaatcattt atgcactatg caataattgg tttagtgaaa cttgtaaaag gagtaattag
   106681 atgaaggtat taattccagc agcaaaagtt gttgaaaaag aattgcagag tataggaatg
   106741 ttaccagcaa ttatctatcc aattaataaa aaaattgtct ttgattattt gtataatcag
   106801 tataatgcaa tctgcgattc attggatatt atctgttatg aaaaagcgga taaggttcat
   106861 agaagactga aaaactatca tgattccaga attcatatat gtgaccttcc tgaattaggg
   106921 gatctagggc aaaccgtgta ttttgggtta tcagatgaag atgagcctgt tctaattaac
   106981 ttcgcagata caattgttat ggagagtatg tctgatatag aaatggatac attctattat
   107041 tctgaagatt ttgtgtcgag tacttggaca tactttaaaa ttaatgaagg aataattact
   107101 gatatccacg ataaagagga agcttacgta tccgataaac gtgaaaaatt atttattggc
   107161 gtatttatgt tttcttcgtc aaaacttctg aagagttgct taagagaggc agttgagagt
   107221 cggaaacagg ggggaataaa ctcattttat cgggctttac agctatacag cgaacgttgt
   107281 ccgctaaagg ccgtaagaac agaaaagtgg tttgatattg gacacatcga taaatattac
   107341 aatacaaagt tggaagttaa agcacgagag tttaatcata tctctataga taaagatcga
   107401 gggatattga agaaaactag cgatgacaag gataaattta ttggagaaat aaagtggtat
   107461 ttaaaacttc ctgctgatat tgaatatgta agacctagaa tttttgatta ttctacatct
   107521 tacgtagctc cctatgttga tatggaatat tatgcatatc atacggttca tgagttatac
   107581 ttatatggtg atttaaaata taaccagtgg catgatctgt ttagccgtat aaagtttatc
   107641 tgtagcgatt ttaagcgata caccttaaaa gatgaaaaga ttagagagtc attggaagat
   107701 atgtatttgc gaaaaaccat gcagagattt caaaaattgt ctgaagataa aagattttca
   107761 gctttttttg agaaacaaat tcaaattaat ggtcgtaagt ttatttcttt aaccggtgta
   107821 attgaacttc taaagaatct tatcccaagt atgctctatg atgtaaatga attttgtatt
   107881 atacatggag atttgtgttt tgctaatatg atggtagatt ccaattgttc ctttgttaag
   107941 gttattgatc ctaggggaaa atttggaaaa tatgatattt atggggacag cagatatgaa
   108001 ttagcaaaac tatttcatag tttagatggt aaatatgatt atatcatcaa ggatctattt
   108061 actgtagaat atgataccga gtgtgcagtg atagattata caattaatac gcgtgtaaga
   108121 gattacgatt tatttgaatt gtttaagtcg gtcttttcgg acgagattaa ggacgatttt
   108181 gataagattg tattgataga agcgttgcta tttcttagta tgattccatt acacggtgaa
   108241 agttttgatc accaaatagt gatgcttgct acaggtatgg aacttcttgc aagagtaagt
   108301 aataaagtta tactctaata taaattgtgc cttaatgaat aacggttaca atactgtttt
   108361 agcaatgtag gcattaaggc acgattatat taattaatac aatagaaggg aagagaaaaa
   108421 tgtatagtga atattctttt gtgtttgata tcgatggcac tttgtgtccg ataaagaaga
   108481 aggatgagcg atatgaggat ttggtgccgt atgagaatat ggttgaaaaa ctgagattct
   108541 ataaggagaa tggtgctaaa attacattgt tcacctcacg caatgttaat tcctataaag
   108601 gaaatttggg aatgataaat gctaatactg caaaagtgct cttggcgtgg ttggataaat
   108661 gggacattcc gtatgatgaa atcctgtatg gtaagccttg gccggggcat aaaggcttct
   108721 atgtagatga tcgaacggtt agacctgatg agtttttgag atgctctgta gatgaattaa
   108781 atgaaatctg caacagttca aaaaaaagat tctaaataag gaggttagtt ctatggaaaa
   108841 aaagatgaca gttatcatta cgatggcagg tttgggaagt cgttttcgaa aaattggtta
   108901 tgcggttcct aaatatatga ttaatgccaa aggaagaact ttatttgatt gggccatgga
   108961 tagtttggtt ggatacaata aaaatgttga taagtatatt tttgttgttc gtcaggaaga
   109021 caacgcttca gcttttataa aggagcattg tgcttacttt ggtattgaaa acgtagaagt
   109081 gattgaaata gactatatga cagatggtca agcaactaca tgtatgttgg ctattcctta
   109141 ttgcaatcaa gacaatgaga ttatggttta caatattgat acatacgtgg aatcaaatga
   109201 gatgaaatac gaagatattt gtggagatgg acatattcca tgctttcatg ctgatggcga
   109261 tcattggagt tttgcaaagc ttaacgatcg aaatgaagta atagaagtcc gcgaaaaagt
   109321 tcgcatttct gataattgta ctcttggtgc gtattatttc tcatcggcaa agctgtataa
   109381 agctttatat gaagagtatt atgcagactc ctctaatatg gagaagaatg aaaaatatat
   109441 agcaccatta tataacttta tgataaaaaa agggttaaag gtgactatta gcatagtaga
   109501 tgcgaaaaag gttcatgtgc ttggtactcc agaagagctc aatgtgtttt tgaatcaaga
   109561 ataaatagtg tttaggagaa aatagcagtg accagtaata atctaattgt aaaaacatta
   109621 agcagatata agaatactcc acttcctgtt aaggctgcat tatggtttat ggtatgtggt
   109681 tttttacaga gaggggtttc aatgattaca accccaattt ttactagagt tttgtctaca
   109741 gaacaatacg gtttatacag cacttatcta tcgtgggaat ccttattgct catgattaca
   109801 tcgcttagtt tgttcaaagc catgatgaat ctttatgtaa aatatgataa tcaagaacat
   109861 attctttcga cagtatccgg attgacttta gtactgacta ttttttggtt tgttattgct
   109921 atttttatta aggatatatt ggcagaaatt ttaggattga caccagttct agttgtctgt
   109981 atgttctcat attttatatt tcaggcagaa atacaatgtt ggtctttaca caaacgatat
   110041 atatatgatt ataagacttt attagtagct acccttgcag ttactgttgg ctcatctgta
   110101 gttggctgtt tagcggttct gtttatatct cctaccgctg aggcgagagc cttaatgaat
   110161 gtcctagtga ctggtgttgt tggcgtggct gtgtatatat cggtatttag aagatgtaag
   110221 gattttaagg ataatgaagt gtggaatttc gcagtagtat tttgtgtatc gttgatgcca
   110281 cactatttat ctgaatttat tctgaatggt tctgataaaa ttatgataaa ttacttatgt
   110341 ggttcgtcag atgttgctat ttacagcgtg gcatattcta ttggtgctct aattaacctt
   110401 gttacaaatt ctgtcaattc cacttttgca ccttatcagt atcaaaaaat caagtcagga
   110461 gaatatgatt tactatcaaa aagagctaat gaagttcttg gtttggtagc ggctatgctt
   110521 tttgcattaa tgctatttag taaagaaatt gtgttgatat ttggtgggca gaaatatatc
   110581 gacagtgtta atttaattgt acctatatgt atcggtattt atttcaacta tatgtttcag
   110641 ctgtttgcta gagtacagga atactttgaa aagaaactca ctgtcgttat tccatcggtt
   110701 ttgtgtgcaa ttatgaatat tgtactaaat tatgttttta taaagttata tgggtatcag
   110761 gcggtagcat acacaactgt attctcatat atgtttttct gcgtcatcca ttatattttt
   110821 tatagaaaag tttgcaaaga actattggat ggaaaaaaca tatatgatgt cagaggaata
   110881 cttttgattt ctgcaatcac gatagctggt ggcatcctaa tttcaataat taataaattt
   110941 gatttcataa aatatttaat tatagcaatt gcttgtgtcg ctgcaattat atttagaaaa
   111001 cgattgctac ttttagtaag gagaattatg agcaaataaa catttgctca tagagagata
   111061 gagaatgata gatattattt ctgctataac ggtaaatgta ttaacagcat tatatcagcc
   111121 gttcttattt gccgtattaa cagcgtatct gtttatgtac tggtatctat ttatagttga
   111181 taactctcga gatcctaaaa acttaaaaga ttctgtatta atctggttga gatattttaa
   111241 agcatctagt aaatttcgca gactcttttt tcttgcgttt tattttactt tgatattatt
   111301 taaaaccttg cttaatcgaa atatgtggat gaacccgctt tcaaacgtta ttggacagtg
   111361 gtcattatat attatagata atgctacagg agagagcaga ttgacaacag aatgcgtgga
   111421 gaacacgatg ctgtttatcc ctttttgcat gttattgtta tggtggcgtg acattaaatg
   111481 tgatattata cgcaccatct atgttgggat taagtatgtg tttttattct cattaagtat
   111541 agaatttaca caactgtttt ttaggcttgg tacatttcaa ctatcggatt tgttttataa
   111601 caccctaggg ggcttaattg gtgctttgct atattggcta ttctaccgat taaataagta
   111661 tatagactta aaatagaact gctttgaatt ttaatatggt taatattttt ctgcagacag
   111721 gattgtgtaa tctcatcaag aatactttac atgccatcta tttttgctaa aacattgttg
   111781 ttatatcttc aagaggtagg gaattttatt gctttaaatg aggtcgagcg aattaaaaaa
   111841 ttggtaaaat atataacatg gtggtagtta tggtaaaagt gtttgatata attaagtttt
   111901 cggatttcag aggatatact gctgttgcat tggataatga gactcttttc tcgattggta
   111961 ttgagtttca cattaagcaa ataaatcaag gatttagtca taataaatat actatgcgag
   112021 ggctacatta tcagagtgga gagcatgctc aaggaaagat agtgatgtgc ctacatggtg
   112081 aaattatgaa tatcgcactt gatattcgac aaggatcttc cacttatgga tgtgtgtatg
   112141 tcaacacatt gagtgaaagc aatggtaaat gtgcgtatat cccacgtgga tatgctcatg
   112201 gctatcttac tatggaggac aatacgctag ttcagtggtg cgtagataat gatttcaatt
   112261 ctaatgctgc caaatgtata agttatgaaa gcgtagatat tgaatggccg gtcagtcggc
   112321 agtgtttcgt tataagtgag aaagacaaaa atgtggaaag attaagcttt tgattaaaat
   112381 gagctgtgga gaaaagaagg atactggaaa ataagaagaa caataaccga gcaaatgctc
   112441 ggttatttct atgaggaata cataaaacta aatttagata tagatatacc cgtggtgcta
   112501 gttaatttta atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac
   112561 ttaatagaac actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag
   112621 aacgtgagtt gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg
   112681 ttttt
//
LOCUS       DAPBBG010000002       107790 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPBBG010000002 DAPBBG010000000
VERSION     DAPBBG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 107790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.002"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            121..393
                     /locus_tag="RD477_000113"
     CDS             121..393
                     /locus_tag="RD477_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632511.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            408..1331
                     /locus_tag="RD477_000114"
     CDS             408..1331
                     /locus_tag="RD477_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7632512.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1471..2223)
                     /locus_tag="RD477_000115"
     CDS             complement(1471..2223)
                     /locus_tag="RD477_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7632513.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2446..2808
                     /locus_tag="RD477_000116"
     CDS             2446..2808
                     /locus_tag="RD477_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDT7632514.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3445..4917
                     /locus_tag="RD477_000117"
     CDS             3445..4917
                     /locus_tag="RD477_000117"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDT7632515.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4929..5753
                     /gene="nadE"
                     /locus_tag="RD477_000118"
     CDS             4929..5753
                     /gene="nadE"
                     /locus_tag="RD477_000118"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDT7632516.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5826..6716)
                     /locus_tag="RD477_000119"
     CDS             complement(5826..6716)
                     /locus_tag="RD477_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDT7632517.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6914..8431
                     /locus_tag="RD477_000120"
     CDS             6914..8431
                     /locus_tag="RD477_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDT7632518.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8403..9692
                     /locus_tag="RD477_000121"
     CDS             8403..9692
                     /locus_tag="RD477_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7632519.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9867..10763
                     /locus_tag="RD477_000122"
     CDS             9867..10763
                     /locus_tag="RD477_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7632520.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10777..11640
                     /locus_tag="RD477_000123"
     CDS             10777..11640
                     /locus_tag="RD477_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7632521.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11646..12209
                     /locus_tag="RD477_000124"
     CDS             11646..12209
                     /locus_tag="RD477_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDT7632522.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12231..13742
                     /locus_tag="RD477_000125"
     CDS             12231..13742
                     /locus_tag="RD477_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDT7632523.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13754..14713
                     /locus_tag="RD477_000126"
     CDS             13754..14713
                     /locus_tag="RD477_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDT7632524.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14799..15878
                     /locus_tag="RD477_000127"
     CDS             14799..15878
                     /locus_tag="RD477_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7632525.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16130..17530)
                     /locus_tag="RD477_000128"
     CDS             complement(16130..17530)
                     /locus_tag="RD477_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDT7632526.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17748..19409
                     /locus_tag="RD477_000129"
     CDS             17748..19409
                     /locus_tag="RD477_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDT7632527.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19366..20064
                     /locus_tag="RD477_000130"
     CDS             19366..20064
                     /locus_tag="RD477_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDT7632528.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20080..21504
                     /gene="araA"
                     /locus_tag="RD477_000131"
     CDS             20080..21504
                     /gene="araA"
                     /locus_tag="RD477_000131"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDT7632529.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21811..22970
                     /locus_tag="RD477_000132"
                     /pseudo
     CDS             21811..22970
                     /locus_tag="RD477_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            23088..24947
                     /locus_tag="RD477_000133"
     CDS             23088..24947
                     /locus_tag="RD477_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7632530.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25057..25491
                     /locus_tag="RD477_000134"
     CDS             25057..25491
                     /locus_tag="RD477_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632531.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25750..25911)
                     /locus_tag="RD477_000135"
     CDS             complement(25750..25911)
                     /locus_tag="RD477_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632532.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(25930..26952)
                     /locus_tag="RD477_000136"
     CDS             complement(25930..26952)
                     /locus_tag="RD477_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDT7632533.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27080..27433
                     /locus_tag="RD477_000137"
     CDS             27080..27433
                     /locus_tag="RD477_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDT7632534.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27445..28611
                     /locus_tag="RD477_000138"
     CDS             27445..28611
                     /locus_tag="RD477_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7632535.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28703..29323)
                     /locus_tag="RD477_000139"
     CDS             complement(28703..29323)
                     /locus_tag="RD477_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDT7632536.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29515..29811)
                     /locus_tag="RD477_000140"
     CDS             complement(29515..29811)
                     /locus_tag="RD477_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7632537.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30015..30665
                     /locus_tag="RD477_000141"
     CDS             30015..30665
                     /locus_tag="RD477_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDT7632538.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30681..32408
                     /locus_tag="RD477_000142"
     CDS             30681..32408
                     /locus_tag="RD477_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632539.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLSSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32405..34297
                     /locus_tag="RD477_000143"
     CDS             32405..34297
                     /locus_tag="RD477_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632540.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            35001..35870
                     /locus_tag="RD477_000144"
     CDS             35001..35870
                     /locus_tag="RD477_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDT7632541.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            36079..37104
                     /locus_tag="RD477_000145"
     CDS             36079..37104
                     /locus_tag="RD477_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDT7632542.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            37143..38093
                     /gene="manA"
                     /locus_tag="RD477_000146"
     CDS             37143..38093
                     /gene="manA"
                     /locus_tag="RD477_000146"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDT7632543.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            38187..39890
                     /locus_tag="RD477_000147"
     CDS             38187..39890
                     /locus_tag="RD477_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7632544.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39892..41925
                     /locus_tag="RD477_000148"
     CDS             39892..41925
                     /locus_tag="RD477_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7632545.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41936..42376
                     /locus_tag="RD477_000149"
     CDS             41936..42376
                     /locus_tag="RD477_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7632546.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42576..43703)
                     /locus_tag="RD477_000150"
     CDS             complement(42576..43703)
                     /locus_tag="RD477_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632547.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43706..44383)
                     /locus_tag="RD477_000151"
     CDS             complement(43706..44383)
                     /locus_tag="RD477_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632548.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44647..46413
                     /locus_tag="RD477_000152"
     CDS             44647..46413
                     /locus_tag="RD477_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDT7632549.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46428..46865
                     /locus_tag="RD477_000153"
     CDS             46428..46865
                     /locus_tag="RD477_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7632550.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46878..48029
                     /locus_tag="RD477_000154"
     CDS             46878..48029
                     /locus_tag="RD477_000154"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDT7632551.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(48091..48789)
                     /locus_tag="RD477_000155"
     CDS             complement(48091..48789)
                     /locus_tag="RD477_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDT7632552.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48782..50134)
                     /locus_tag="RD477_000156"
     CDS             complement(48782..50134)
                     /locus_tag="RD477_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDT7632553.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50357..51757)
                     /locus_tag="RD477_000157"
     CDS             complement(50357..51757)
                     /locus_tag="RD477_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDT7632554.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51918..52655
                     /locus_tag="RD477_000158"
     CDS             51918..52655
                     /locus_tag="RD477_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDT7632555.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52780..53259
                     /locus_tag="RD477_000159"
     CDS             52780..53259
                     /locus_tag="RD477_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDT7632556.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53256..54023
                     /locus_tag="RD477_000160"
     CDS             53256..54023
                     /locus_tag="RD477_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632557.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            54253..54822
                     /gene="lepB"
                     /locus_tag="RD477_000161"
     CDS             54253..54822
                     /gene="lepB"
                     /locus_tag="RD477_000161"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7632558.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54926..55528)
                     /locus_tag="RD477_000162"
     CDS             complement(54926..55528)
                     /locus_tag="RD477_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7632559.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55634..56103
                     /locus_tag="RD477_000163"
                     /pseudo
     CDS             55634..56103
                     /locus_tag="RD477_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            56209..56793
                     /locus_tag="RD477_000164"
     CDS             56209..56793
                     /locus_tag="RD477_000164"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDT7632560.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56796..57869
                     /gene="prfA"
                     /locus_tag="RD477_000165"
     CDS             56796..57869
                     /gene="prfA"
                     /locus_tag="RD477_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDT7632561.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57862..58701
                     /gene="prmC"
                     /locus_tag="RD477_000166"
     CDS             57862..58701
                     /gene="prmC"
                     /locus_tag="RD477_000166"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDT7632562.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58694..58873
                     /locus_tag="RD477_000167"
     CDS             58694..58873
                     /locus_tag="RD477_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632563.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58895..59920
                     /locus_tag="RD477_000168"
     CDS             58895..59920
                     /locus_tag="RD477_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDT7632564.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60095..61339
                     /locus_tag="RD477_000169"
     CDS             60095..61339
                     /locus_tag="RD477_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDT7632565.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61492..62439
                     /locus_tag="RD477_000170"
     CDS             61492..62439
                     /locus_tag="RD477_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7632566.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62573..63202
                     /gene="upp"
                     /locus_tag="RD477_000171"
     CDS             62573..63202
                     /gene="upp"
                     /locus_tag="RD477_000171"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDT7632567.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63341..63583)
                     /locus_tag="RD477_000172"
     CDS             complement(63341..63583)
                     /locus_tag="RD477_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632568.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63704..63776
                     /locus_tag="RD477_000173"
     tRNA            63704..63776
                     /locus_tag="RD477_000173"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63737..63739,aa:Thr,seq:cgt)
     gene            complement(63895..64533)
                     /locus_tag="RD477_000174"
     CDS             complement(63895..64533)
                     /locus_tag="RD477_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7632569.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64656..65288)
                     /locus_tag="RD477_000175"
     CDS             complement(64656..65288)
                     /locus_tag="RD477_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDT7632570.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65639..65938
                     /locus_tag="RD477_000176"
     CDS             65639..65938
                     /locus_tag="RD477_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7632571.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66433..68598
                     /gene="acm"
                     /locus_tag="RD477_000177"
     CDS             66433..68598
                     /gene="acm"
                     /locus_tag="RD477_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDT7632572.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68870..69355)
                     /locus_tag="RD477_000178"
     CDS             complement(68870..69355)
                     /locus_tag="RD477_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDT7632573.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    69445..69790
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            69835..70476
                     /locus_tag="RD477_000179"
     CDS             69835..70476
                     /locus_tag="RD477_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7632574.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70495..71130
                     /locus_tag="RD477_000180"
     CDS             70495..71130
                     /locus_tag="RD477_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7632575.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            71148..71963
                     /locus_tag="RD477_000181"
     CDS             71148..71963
                     /locus_tag="RD477_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7632576.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72282..73718
                     /locus_tag="RD477_000182"
     CDS             72282..73718
                     /locus_tag="RD477_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7632577.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73907..76354
                     /locus_tag="RD477_000183"
     CDS             73907..76354
                     /locus_tag="RD477_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDT7632578.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76584..77651
                     /gene="rlmN"
                     /locus_tag="RD477_000184"
     CDS             76584..77651
                     /gene="rlmN"
                     /locus_tag="RD477_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDT7632579.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77844..78947)
                     /locus_tag="RD477_000185"
     CDS             complement(77844..78947)
                     /locus_tag="RD477_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7632580.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79500..80048
                     /gene="aac(6')"
                     /locus_tag="RD477_000186"
     CDS             79500..80048
                     /gene="aac(6')"
                     /locus_tag="RD477_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDT7632581.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            80594..82255
                     /locus_tag="RD477_000187"
     CDS             80594..82255
                     /locus_tag="RD477_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7632582.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(82325..82672)
                     /locus_tag="RD477_000188"
     CDS             complement(82325..82672)
                     /locus_tag="RD477_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDT7632583.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            82756..83697
                     /locus_tag="RD477_000189"
     CDS             82756..83697
                     /locus_tag="RD477_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632584.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            83698..84741
                     /locus_tag="RD477_000190"
     CDS             83698..84741
                     /locus_tag="RD477_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632585.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            84747..85817
                     /locus_tag="RD477_000191"
     CDS             84747..85817
                     /locus_tag="RD477_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632586.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            85807..86760
                     /locus_tag="RD477_000192"
     CDS             85807..86760
                     /locus_tag="RD477_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7632587.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            86772..87782
                     /locus_tag="RD477_000193"
     CDS             86772..87782
                     /locus_tag="RD477_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7632588.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(88067..88888)
                     /locus_tag="RD477_000194"
     CDS             complement(88067..88888)
                     /locus_tag="RD477_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7632589.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(88985..89263)
                     /locus_tag="RD477_000195"
     CDS             complement(88985..89263)
                     /locus_tag="RD477_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632590.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            89531..91540
                     /gene="metG"
                     /locus_tag="RD477_000196"
     CDS             89531..91540
                     /gene="metG"
                     /locus_tag="RD477_000196"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDT7632591.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(92019..92606)
                     /locus_tag="RD477_000197"
     CDS             complement(92019..92606)
                     /locus_tag="RD477_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632592.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            92781..94103
                     /locus_tag="RD477_000198"
     CDS             92781..94103
                     /locus_tag="RD477_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7632593.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            94258..95034
                     /locus_tag="RD477_000199"
     CDS             94258..95034
                     /locus_tag="RD477_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDT7632594.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            95027..95596
                     /gene="rnmV"
                     /locus_tag="RD477_000200"
     CDS             95027..95596
                     /gene="rnmV"
                     /locus_tag="RD477_000200"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDT7632595.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            95589..96473
                     /gene="rsmA"
                     /locus_tag="RD477_000201"
     CDS             95589..96473
                     /gene="rsmA"
                     /locus_tag="RD477_000201"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDT7632596.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            96924..97553
                     /locus_tag="RD477_000202"
     CDS             96924..97553
                     /locus_tag="RD477_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632597.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(97677..98786)
                     /gene="ugpC"
                     /locus_tag="RD477_000203"
     CDS             complement(97677..98786)
                     /gene="ugpC"
                     /locus_tag="RD477_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDT7632598.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            98995..99417
                     /gene="mgsA"
                     /locus_tag="RD477_000204"
     CDS             98995..99417
                     /gene="mgsA"
                     /locus_tag="RD477_000204"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDT7632599.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            99740..100276
                     /locus_tag="RD477_000205"
     CDS             99740..100276
                     /locus_tag="RD477_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDT7632600.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(100316..102079)
                     /locus_tag="RD477_000206"
     CDS             complement(100316..102079)
                     /locus_tag="RD477_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632601.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(102082..103797)
                     /locus_tag="RD477_000207"
     CDS             complement(102082..103797)
                     /locus_tag="RD477_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632602.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(104576..104800)
                     /locus_tag="RD477_000208"
     CDS             complement(104576..104800)
                     /locus_tag="RD477_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632603.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            104991..107768
                     /locus_tag="RD477_000209"
     CDS             104991..107768
                     /locus_tag="RD477_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7632604.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFKQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT        35798 a        18774 c        22055 g        31163 t
ORIGIN      
        1 taaaataaga gactttggta ataatgaaag aggaataaaa cttttctttg gcaaaacaaa
       61 ctccttgcta tgagacgcgt ttcatacact atactcaaag tatcaaaagg agggcttgca
      121 atgaatactt atgtagagat aacagatggc acaacgaaca attattttta tgcgtttgtc
      181 gagttcaagg gatccgtatt aacgttgtac agttttcaag ggttgaatag gaatatagta
      241 aaagaaatac caatgaatga aatcgaaaat ttgacaaaag atatatattg gggcgggcag
      301 cggatcagtt tttcgcatga aggaaaaatg tatcagtttt ttgaatgcgg tccagctgtg
      361 gtagactatt tacaagaaaa tctttttgta taggtggcga agcagcagtg gcaaacattc
      421 gagatatcgc aaagctcaca ggttattcag tttctactat ttcacgtgta atcaatggac
      481 atccatatgt agatgaggaa aaaaggaaag aaatacttgc ggtcatgaaa gaagtcgatt
      541 atatccctaa tgcaaatgcc agacagttaa gttatggaaa aacgaaaagc attggagtga
      601 ttctgcctta taccaatcat ccgtattttg atcagttgat cagtgggatc attgaagcag
      661 cgtttgatta tgattacaaa gtgacacttt taccgacggg ctatcataaa gaaaaagagc
      721 gaaggtatct tgaagaattt gcagctaaag ctttcgatgg attgatcatt acgtctcgtg
      781 ccaatacact agatgattta ttggcatatc aaaaatatgg acctctagtt ttttgtgaag
      841 aaatcaaggg aaaacaggca agctgtgtct ttattgatcg agaacaatca attacagaag
      901 gtcttactta tttgaagcag caaggtataa agcaattggg catcacgttg gggcgaagtg
      961 gcaggctaag ttataactca aaaatcactt tgcagctatg tcatcaactt tttccatctt
     1021 ttgatgaatc acttgttttt tgggattgta tagatgcaga gaaaggaaaa caagcaggct
     1081 tcttttttaa agagcttgga gttgatggtg tctttacaaa ttcggatatg gttgcagcag
     1141 gcatcttgca aagctatctg caagataaga caccagtggt gattggacgc gataatttgc
     1201 tgatcagcga gcttcttggt ttcccaacca tcgaccacca tctggaagct tgcggagaaa
     1261 tggcatttca attgtttctt acagaaaaaa aggataaagt gaaaatacct tatacgttta
     1321 ttcaacgatg aatatattta aagtaggatg aaaaaacttg tgaaagaatg gagcagacac
     1381 caaaagttag cataaaaatc taacttgggt gtctgctgtt ttgttattag gtcattttac
     1441 gtgtactttt ttgttggatc atgtaaaaga ttacgccttt ttttggataa tttcatttaa
     1501 agcttttgct gcaccatgac cacttcgctc gtcataatat gcagtgaacc gttcatcggc
     1561 ttcgtacatc attcctagtc ctttatgcgc ttttggctga tattcaggcc agctgtaaga
     1621 gaaccacttc ttatgagctt ggaagatacg gtctgctaag ctttcatctt ctggttcttt
     1681 taagtaaaga gtgagatcaa ataacatctg cttttcaaca gtttgcattt cttcatacgt
     1741 ttctttatcc atattcatcc atttttcatt ggatgctgta accttttttt ttccgtattt
     1801 ttgtcgaatc tcttgtccat aggattcttc attttctttc aacttttgtt gtttaaaccc
     1861 ttcaaatttt tctttgtcac tcatttttct tcctccttcg tacatatcaa gtgtctgttg
     1921 gacagttgtc aagagggtat cgatttgtgc ccgtttttcc agcaacattt gctgatgctt
     1981 gattaaagct ttctggtggt caaaagatgg attgtctaag acctcttgga tatcatccaa
     2041 cttgaatgcc attgttcgat aaaataagat gtgttgcaga cgatcgactt cttttttgcc
     2101 ataaatccga taacctgaag aattgatcct agctggtttt agcagtccaa tctcatcata
     2161 aaagcgcaag gttctcggac tgactcctga tatctctgac attttcttta ttgtgtactc
     2221 cattattttt cctcctgaca aatgtaagca taatccttta cgtaacgaga tggtcaagaa
     2281 aaaattgtat ttttttctca cgaaattatg ttatactatt aagtgataaa aggtaaatcc
     2341 ttttgtcttt aaaggttcaa ttgtaaccga ttccttaatg ctaggaaaga tggtaacata
     2401 acttaagatt ggatctaaat cgactatata gaaaagagga atgagatgaa gctaatcgaa
     2461 acacctgtga atagtaattt aaatattaag acattttatc ctaaagtagt ggaattcttt
     2521 tttgggaata cggctattaa ttattacaaa ttattttcgt tagatcgaac acaactgcta
     2581 ttggtagaca catatgataa aaagcaagtc gtgatgatca atacgaagaa aaaaatcaca
     2641 aggcaagaaa tcgactatgc gattcatcac gttttaaaaa tgacacgaga agatgtcaaa
     2701 gtccatgtag gagtgaaaca ggagctagaa cgtgccggaa tacaattcaa acgtccaaat
     2761 aaagatattg tagtgataga acaaaaaaat acgaaggatt ctatttaata acgactaata
     2821 atcatcttta tggtaacctg ctccatatct aatgtataca ataaaaaaca aggcgagact
     2881 gattgatgtc tcgccatttt tgtttccatt ctattgacag ctgctttctt actttgtatc
     2941 tttttaaaaa cgtgtgatat tttttccgcg agttttttca acgcaaggta tatcttgtca
     3001 taattcgcct ttccaaaaaa attcaataca taataagtta tatccgtttc tttggggata
     3061 agaagcaaaa aaggaaagac gtattattgc ttcaaaaact tttgccattg attatgttgt
     3121 agagacattt ggttatattt gttcaatcat cggtatctta acggctacga ttgtagtagg
     3181 aaaatctact tattttatca agtaagaaaa atagaaaacg ctaatttctt cgtgtctcgg
     3241 atacattgaa taaaataagt attctttctc gtttaggtta aataccaata agaaaggata
     3301 gttttagcag atggagttca tttttagcaa gaaatatccg taaaacatgt gctttttcag
     3361 aaaaaatgga agatttcttt tcctattaat tttttttgtt ataatgggta ggaaatgaaa
     3421 acttgccaag aggagtaata caagatgaat aatacttacc cagacgacag tttgacgctg
     3481 catacggatc tatatcaaat caatatgatg cagacatact gggaattagg tcgagctgat
     3541 ttacatgctg tttttgaatg ttactttcga gaaatgcctt ttaaccacgg atacgcggtt
     3601 tttgcaggat tggaacggtt agtcaattat ttagagaatc tgacgttcaa tgattccgat
     3661 attgcttact tgcgtggatt agaagtctat cctgaaggtt ttttggagta tttacaaaat
     3721 tttgaattta aagcaacagt tcgttctgct cgtgaaggag aactagtttt tgcaaatgaa
     3781 ccattaatcc aagtggaagg gcctttagcg cactgccaat tagtagaaac agcactgttg
     3841 aatatggtca atttccaaac gttgatcgcg acaaaagcag ctcgtatcaa atctgtcatc
     3901 ggagaagatc cactattgga atttggaact agacgggcac aagaactgga tgcagcagtc
     3961 tggggaacta gagcggcata tatcggcgga gcagatgcaa caagcaatgt acgtgctggg
     4021 aagatatttg ggataccagc tagcgggacc catgctcatt cgttagtcca atcttatgga
     4081 aatgattatg aagcatttat ggcctacgct aaaacccata aagactgcgt gttcttagtc
     4141 gacacttatg atacgttgaa atcaggtgtg ccaagtgcga ttcgcgtggc gaaagaatta
     4201 ggtgacaaaa tcaacttcca aggcgtacgc atcgacagtg gagatatggc ctatatatct
     4261 aagcgtgttc gggaacaatt agatgcagcc ggatttacgg aagcaaaaat ctatgcttcg
     4321 aatgacttgg atgaagcaac gattttgaat ttaaagatgc aaaaagctaa aattgatgta
     4381 tggggtgttg ggacaaaatt gatcacagcc tatgatcagc cagcccttgg tgcagtattc
     4441 aagctagtat cgatcgaaga tgatgaagga aaaatgatgg atacgatcaa gttatcaagc
     4501 aatgcagaaa aagtaactac tccagggaaa aaacaagtgt ggcgcatcac tcgaaatttt
     4561 gatgggaaat ctgaaggaga ctacgtgaca ttatgggatg aagaccctcg agaagaagaa
     4621 gctatcttca tgttccatcc agtgcatacg tttatcaata aaactgttcg agactttaca
     4681 gctcgcccag ttttacaaga tatctttatc gaaggaaaaa gagtttacga attacctgca
     4741 ctaaacgaaa tcaaagaata tacaaaagaa aatctagatt cactatggga agagtataaa
     4801 cgagacttga atccgcaaaa atacccagtc gatctgtcta ctgaatgctg gaaccataaa
     4861 atggctatca tggaacgaat gaaaaaaagc gtagcagaac ttcatacaga agcataggag
     4921 gagaataaat gagcctgcag acagaaatta tcaatgaatt aggtgtcaaa ccaacaattg
     4981 atccaaaaga agaaatacgt aaaagtattg actttatgaa agcatatttg aaaaaatatc
     5041 cgtttcttaa aacttttgta ctaggtatca gcggcggcca agattctact ttagcaggcc
     5101 gtttggcgca actaacgatg gaagaaatgc gtgaagaaac aaaagatgaa aactatcaat
     5161 ttatcgctgt tcgtcttcct tatggtgaac aagcagatga agaagatgca aaagctgctc
     5221 ttgactttat ccagcccgat gttagtctgc gagtaaatat caaaccagct gttgatgcac
     5281 aagtacaagt attaagcgaa gcaggtgtgg gaatcagcga ttttaacaaa ggaaatatca
     5341 aagcacgtca acgtatgatt acgcaatatg ccgtagctgg agaaagagca ggagccgtat
     5401 tagggacaga tcatgctgca gaaaacatta ctggattctt cacgaaattt ggtgatggtg
     5461 gagccgacat tttgccatta ttccgtttgg acaaacgaca agggaaacaa ttgcttcaag
     5521 cattgaatgc accggaaaaa ttatacacaa aaattccgac tgcagatctt gaagatggga
     5581 aaccaatgat tgcagatgaa gtcgcactag gtgtcacata taatgaaatt gatgattatc
     5641 tagaaggaaa agaagttccc gctcaagcac aagaaaaaat cgaagcatgg tggaacaaga
     5701 cacaacataa acgacatttg ccaatttccg ttttggatga tttctggaaa taacaataaa
     5761 gcaaaaacag ccgtcccttc atagggattg gctgtttttg ttttataaaa tagaaaagaa
     5821 aaatatcagc tgtagtaaac ttttaatttg atatcctgat cgtaattacc gaatccttta
     5881 ccaaataaag tacagccccc cacatgtttg gcatcgtctt tcacttcgat tcgcaaatgc
     5941 caagtgtctt gctctttagg gatgtcgttg atcgtgatgt ttgacaaggg ttctccattc
     6001 atattcgtca agtgtttggt aattcggatc gtcttcagca agccgtattg attaagatta
     6061 tctggatacc aatcaggcgt atatttaccg cggatatcag caaaatctcc agggctcgtc
     6121 catgttccta gctccacacc atttaggctg aaagtgatat ctgaaggcca attgtcattt
     6181 gaaaaaggaa attcagaaga aatttccaca gaaacttcca acatctccaa tgtatcctct
     6241 gtatttaaaa aattaggtgc ctgatactcg acaaatccgc tggtaaacca gagaatgcgg
     6301 gcatccatcc ttcgaggatc cataaaatag cgtggttcat ccactttccc gataaaatca
     6361 tggatcgtag ctaaaccgca tgttggttca attgcataat ttgtatagtg accaataggt
     6421 atcgatgttt ctttcgtatc aaaagcattg aatatttttt ctggaaaact gatatcgatt
     6481 ttatcgacac gaaggctcga aattttttgt tgtcctactt tttctgtttt gatgatgttt
     6541 gcttcctcta gctttttcac gtgcatcgtc gtgatagcac tgctcactcc aagttctttt
     6601 gccaaatctt tgacattcat cttttttttg gaaagtagct ggatgatctt gatccttgtt
     6661 ttactagcta atgcttcata taccggtaag gattcttcgt ttatttgtag ttgcatatat
     6721 atcacctctt aaaaggtttt tcttatatat taacatattt gttaataaaa gttatattaa
     6781 attatttgtt ttaatcatta ttaatatttt ggttaattaa aatcgataaa aaataagtaa
     6841 gtttaacatt tatattattg acaacgcttt caacgaggtg tattattcat gtataaatta
     6901 aaggaggtca gttatggaag ccacaatgtt gattagtagt gtcaagcgtg taggcaaagt
     6961 tgatccgcgg ttgtacagtt catttattga acatatggga cgagcagtat atgaagggat
     7021 ttatcagcca gatcatccat cagctaataa aagtggattt cgccaagacg tcattcactt
     7081 ggtaaaagaa ttgaacattc ccatgattag atatcctggt ggaaattttg tgtcaggatt
     7141 tcgttgggaa gacagtattg ggcccatagc aaatagacct agaaggttgg atttagcttg
     7201 gcggacaatt gaaacgaatg aagttgggat tcatgaattt tataactggt gtcagcatgt
     7261 gggggccgaa atcaatatgg ctgtcaatct gggtactcgt ggagtagatg cagctcgtaa
     7321 tttagtcgaa tattgtaatt ttcctaaagg aacttattgg agtgatttaa ggcgttcgaa
     7381 tggtcaagag agcccttttg gaattaagac atggtgttta gggaatgaaa tggacggtcc
     7441 ttggcagatt ggacataaaa cagcggaaga atatggacgg ttagcagagg aaacggctaa
     7501 ggcgatgaaa ctcgtagatg attcaattga ggtggtttta tgcggaagtt cgaatcggca
     7561 aatgcctaca tttggtgatt gggagttaac agttcttgat caagcgtacg atcaaataga
     7621 ttatttgtct ctgcatcaat attatggcaa ccaaaaaaat gatctttctc attatcttgc
     7681 acgttcctta gacatggatc ggttcatttc tgaagtgaca gcgatgtgcg atacagtcaa
     7741 ggcgaagaaa catagtaaga agcagatcaa cctgtccttt gatgaatgga atatttggta
     7801 tcattcagcg gaacaagatg agcaagcagc gccttggcag attgctcctc ctttactaga
     7861 agataactat aattttgaag atgcattact attaggctgt ctattaatca ctttattgaa
     7921 acatgcagat agagtaaaga ttgcttgtct tgctcaattg gtaaatgtta ttgcaccaat
     7981 caggacagaa acaaacggag aagcttggag acagacgatc tattatccat ttatgcaagc
     8041 tgctgtgtat ggacaaggag aggtgttaag cccacaaatt cgttcagcaa actatgcgac
     8101 ggaagatttt gaagaagtgc cgtatttaga aagtatagcc gtaataaatg acaaagaact
     8161 tgtcatattt gcagttaacc gtggagaaga agaaatgaat ctaactgttc acttgtctga
     8221 attatcatta gagggagtga ttgatttttc tgaaatgagt ggatttgata caaaagcaac
     8281 taatcttcct aaaagtgaac aagtaaaacc aagtcattca acaaatgtaa aagctgaaga
     8341 tgggaaaatc aattgtttat tgaaaccgct atcttggaat gtggtcagat gtcgtttatc
     8401 agatgaagga ggaacaaaaa atgaaaggta aaaaatggtt aattggctct ttgttggtag
     8461 ctgcaacagg tattttagga gcttgcggtg gcggaagttc agcagaatct gattctgata
     8521 aagaagtgac tttgactttt tggaatgggt ttactgcatc tgatggtgaa atattgcaag
     8581 aaatcgttaa tgaatttaat aaaacaaatg ataaaaacat caaaattgaa atggatatca
     8641 tgacttgggc caatttgaat gaaaaacttc ctacagcgat ttcttctaaa aaagcacctg
     8701 actttattgc attaaattat ccagattttg cacagtatgt agaaaacgga gcggtacaac
     8761 cattagacga tttctgggaa tatgatggag tagataaatc tgattttaaa gaaacggcta
     8821 ttgaattagg tgaagtagaa ggtaaacagt attttgtccc catgcaggta caaggcatgt
     8881 atatgtattg gaataatgat ttgtttaaac aagctggatt agatactgaa caaccaccta
     8941 ctacttggga agaattgaca gaaatggcac caaaattaac tgatagtagt aaaaatgtat
     9001 caggttttgt cttcaataag gatggaactg caccgttata taactggatg ttagcaaatg
     9061 gcggtaaatt agtgaacgat gattatacta agtctgaatt tgcgtctcct gagaacctta
     9121 aaacgttaaa agcgatccaa aaaatgatcc atgtagacaa gactggtcca gaatcgattt
     9181 ctggcgccga aatggacaat ttaatgaatg ctggtcaact agccattgaa atcaatgggc
     9241 catggttaaa taacggattg aaagcaaacg agattgatta taatgtgact accttaccac
     9301 aagggagtga ttcagacaaa aaaacagcta ttttagatgg cgtaggatat gccattccgg
     9361 caagtacaga taataataaa aaagaagctg tctatgagtt tttgaaatat tggaatacaa
     9421 ctgaaattgg taaaaaatgg agtatcgaaa atggttttcc tgcctacttg gattcagtag
     9481 caaaagatga agaagtaaaa aatgatccta ttgtatctga attatcgaaa caaatggact
     9541 atgcagagcc atatttacca ggttttaccc aaatctctgc gatcaataac gatatcatca
     9601 atccactgat tgaaaaatta ttagcaggag aagatcctga gacactcatg aacgatgcag
     9661 atgatgccat caatcaatta ctgcaaaaat agtacagaac tttatacagg atagactgtt
     9721 ggcaagatac agatatctga atgatcgacc tagtatttgc agataggtgt tattgcaaaa
     9781 atcacagcga ttgttcggat atcttattca gattcgttta aaccaagatt gtatatttta
     9841 tcaaaatgaa agatgaggtg gactatatga acggattcac caaatattgg aatcgtcctg
     9901 atcgtgctgc gtatttgttt ttaactccat cttttttagt tttattatta tttactgtca
     9961 taccgctttt aggaacattt gctattagtt ttacagattt gaatattttc tttacatcaa
    10021 gaaattttgt aagttttgaa aatttcacac gtatatttag tgatgaacga gcagttaatt
    10081 cgttgattca ttctttatat ttcaccgtac tcgaaacgcc aacacaaatt atcgttggtt
    10141 tattagcagc agtagcatta tctaaaaata ccagattcaa tcgtttttgt cgttcgacat
    10201 tttacattcc agttatttgt tcattgacgt caattgcgat tattttttct atgttgcttg
    10261 atccgaatgt aggggcgatt ccttacttgt tttcacaagt cggcttgccg atccctcaat
    10321 tttttcgcga tccaaccctt gctatgccga cggtagccat catgactgtt tggaagaatt
    10381 ttggtgttac tatgacgatt ctcttgactg ctattcaggg aatctctccc tctttgtatg
    10441 aatctgctca aatggatggt gcaacaaatc gacagcagtt ttttaacatt acgctacctc
    10501 agatcgttcc ctccttaggt ttttgtattt taacgaatct gattggttcg atgcaagtgt
    10561 ttgaccaagt atatgttgca accgctggtg gcccacaatt taagacagaa acagctgttc
    10621 aatatattta cagtcgagga tttacagcac cttatgaatt gggctatgca tcagcactgt
    10681 cttcggtttt gtttatcatt gttgcaacta tagctatctc cactaatgtg tacatgtcac
    10741 ggaaagaaaa acaaatgaaa taggagggaa acctgtatgg caccagaaag ctcgattaca
    10801 gttactgtga atagtcgaaa agcagttagt ggtaaaaaaa tcattcgaac gattagtatg
    10861 ctagttatat tgttggttat tgcgtttcct tttttgtggc tgattatttc ttcttttaaa
    10921 cacgaaaaag acattatctc atttccgcct agaatatttg cagatagcta taccctagat
    10981 aactatatta aggtttggac aacgatccct ttattagatt acatcaaaaa tacagtgatt
    11041 tttgctggag ggacagtgat cacttctgta ttctttgatt ctcttgcggg gtatgcattt
    11101 gctcgtatgc gtttcaaagg aaaatcagtt ttgttctatt tcgttttgct gacaatgatg
    11161 atcccattcc aagtcttcat gattcctcta tttattgaag tcaatttgct tggcatgctt
    11221 gatacttatg caggtttgat cattcctcgt atgacaactg catttggtat cttcatgatg
    11281 cgttcctttt ttattacttt accagattct ttggaagagg cagcacgcat tgatggatta
    11341 agtgagttta atattttttt gaaaatcatg ttgccattga gcaaacctac tcttttatca
    11401 ttagggattt tcacgctgat gaatagttgg aatgacttgc tttatccatt gatattgaca
    11461 agtagttcaa aaatgagaac tttaccagca gggttagctt tatttacagg gcagaacatt
    11521 tctttttatg gacctgtaat ggctggaacg gttatttcaa tgctgccatt actagtggtt
    11581 tatatttttg cacagaaata ttttgttcaa ggaacagcta tgtctggaat gaaagagtga
    11641 ggaagatgaa aatagttgag actaaacttt atggacagtt gattgtggtt tctgattaca
    11701 tcttattagg tattctctgg gtaatcgtgt gtttgcctgt tattactatc gtgcctgcta
    11761 catgtgctgt tttttatgta atggcgcaat ggaaaggtga gggatcagga aatatcattt
    11821 attttttctt tcaaggaata aagcaacatt ttggtaagaa cttgttcctt agtctgttaa
    11881 cgatcattat ttattttctt gttaatcaat tattagaaga acagacaatc gtactttcaa
    11941 ttagtggata tgtagtttta cttatttatt ccatgtttct tttatcctgg atatatgtga
    12001 tttctgaaaa caaagatatc acatttttac aaatttttga acaaagtgca tggaaagtat
    12061 tttttgattt tttgagaaat attctttgtt gcgtattgtt attaggattt gtactgctca
    12121 tatttctttt ccctcctttt atttttatct ttgctggtgg cgtatggaaa cttgtatcta
    12181 ttatcttgac tataggaaat gaaaggtagg gaaagctgtt ggacgaagaa gtgaaagtaa
    12241 aaatcagaga ggattttacg gtaggagaga tcagttctcg gctgtttggc tcatttgttg
    12301 agcatatggg aagtgtcgtc tatacaggga tttatgaacc gggccatgaa acagctgatg
    12361 gtgacgggtt tagacaagat gtcctctctt atgtaaagga attagaatta ggtgtcattc
    12421 gctatcctgg aggaaacttt acatctggtt atgattgggt ggatactgta ggtaggaaag
    12481 aacaacgacc aaaaaaaatt gatttagcat ggcgaggtat agaaccaaat tcttttggac
    12541 tccacgaatt ttttaaatgg atcagtcatg tacaagcgaa accgatattc acggtgaatt
    12601 taggaacaaa aggaattgat gaagcaaaaa atttagtaga atattgtaat tttccaaaag
    12661 gaaccttctg gagtgattta agaataaaaa atgggcaaaa aaagcctttt ggcgttaaat
    12721 tatggtgttt agggaatgag ctagacggtc catggcaagt gggggctaag agtgcttatg
    12781 aatatggtag gttagcaaat gaagcctcaa aagtcatgaa gttagtggat gattcaattg
    12841 aaacaatctt agttggaagc tctacgccac gtttaccatc gtaccctgaa tgggatcgta
    12901 tcgcattaga agaagcttat gagaatgtag attatttagc tttgcataac tatattgata
    12961 agtatgatga agaagacttg actaaacctc ctaaaaatga gcgtgatgat acagcgacat
    13021 accttgcaaa agcttatcga ttcgatcgtc aaattgaaga aatcgttgct acttgtgatt
    13081 atgtaaaagg atatttgaga agtgagaaaa caatgtatct agcgtttgat gaatggaatg
    13141 tacatgccca accggaaaaa agacataggg attttgaagt cggtagtccc atcgattggt
    13201 gttactttac tatggaagat acattattat ttggttcatt agggctggct attttacgac
    13261 acgcagatcg cgtaaagatt agttgtcaat cattgttagt aaacacaata cctttaatac
    13321 tgacagatgc taaaggacaa gcatggagga atccaactta ttatgtgatg caacacttat
    13381 cgaaatatgc taaaggtcaa gtattgaaac aacaaatcat ttgccctgtt tatgattgtg
    13441 aaaagataaa aaatgttcct tatgtggaca gtgtgatcgt agataacggc aatcagctga
    13501 ctattttctt gatcaatcgc acaaatgaac aacaaaagat aacgattgaa catgatttca
    13561 atttgctaga tcaaggagag cactacacat taaccagtag cgatctgttg gacaaaaata
    13621 cacgtgaaga accagaaaaa ataaaacctg aaataaacta tttttcaata gaaaaacatg
    13681 acgaattaaa tgtagggata gaaaaatact cttggaatgt aattagaata aagaaaatgt
    13741 gaggaaattt gttatgatga agacacttta taataatcct atcgttcggc aacgggcaga
    13801 tccttgggta tataaacata cagatggtta ttattatttt acaggatcgg ttccaggcta
    13861 ccaagtgatt gaattaagaa gggcaaagtc actgaatgaa ttagaacatg ctgcaagcct
    13921 gattgtatgg caggctcatg aagaaggacc gatgagtgaa ttgatctggg cacctgaagt
    13981 ccattatatc aatggaaaat ggtatattta ctttgctgca agcaatcata aaactatacg
    14041 tgatgaatct catcatcacc gcatgttcgt acttgaaaat cagcatatag atccaatgaa
    14101 tacagattgg atagaaaagg gacaaataaa aacgaaaagg gaaactttca gtttagacgg
    14161 aacaactttc tcttctcaag gaaagctata ttatgtgtgg gcccaacaag atcctgaaat
    14221 tactgggaat tctaatcttt atctatccga aatggataat ccttggacgt taaaggggaa
    14281 gcagatcctc ctttctatac ctgagtatga atgggaaaaa cggggttttt cagtaaatga
    14341 aggtccagca gtccttactc gcaatgggaa aatattcatt acgtattctg gtagtgcaac
    14401 agatgaaaac tatgccatgg gcctgctgtg ggctgatgag cacaagaatc ttttattaaa
    14461 agaatcatgg cataaattat cggagccagt attcgtttct tctgaaaaaa ataagcaatt
    14521 tggtcctggt cacaactcgt ttacggtttc agaagacggg tgctttgata ttttgatcta
    14581 tcatgctcga ccagagaaga accaaaaagg ggatccgtta gacaatccta atcggcacgc
    14641 gaacgcgcaa tgctttacgt ggaatgagca aggatttcca gaatttggtg aacctgttcc
    14701 gtatgcctta tgagcacaag aaaccttgat ttttgcttga ataaaccttg cagactcgtt
    14761 atgatatatg tacgtacaaa taaaaagggg caaggcaaat ggcaaaatat caagatattg
    14821 cagaagagat aaaacaaaag atccttcgtg aggaatacga agtaaataca accattccct
    14881 cagaattcaa gctgcaagaa atatatgacg tcagcagaca tacgattcgg caagcaatcg
    14941 ctgtgttggt aaatgagggc tatctgcgta aagaaaaagg gtcgggcaca tatgtagatg
    15001 acagctataa gaaagaaaaa acaccattga acagaacaaa aacgatcggt gtcatcacca
    15061 catatctctc tgattatatt tttccgtcga ttatccgagg aatcgaaaat gaattacgag
    15121 aatctgggta ttcattgctt ttagccagta cgaataacga tcacgaattg gagaaagagt
    15181 gtttgaagaa aatgattgct tatggggtag atggtttgat cgttgagcct acgaaaagta
    15241 atcagtataa cccaaactta gcactttatg ttgctttgag agaacaaggc attccgatgg
    15301 tgatgatcaa tgctgcttat gaagagttaa atgcctctta tatttgttta gatgatgtga
    15361 aagtcggttt tatggcagca gaatatttga taaaaaaagg acatcagcgt ctgttgttca
    15421 tcacaaaaat cgacgatctt caaggaaaat acaggatgaa aggttttatc agcgcgtgtg
    15481 aacagtataa tgtgcgtttt ttgacggaag atatcattac tttcaccacg gaaacacgag
    15541 aaacaatcgc tgaaaaaaca gtacaacggt tgaaagaaga acaagcttta gatggcatca
    15601 tttgctataa cgatcaaata gctagtttaa tgctagataa gttgacagaa aatggctatc
    15661 atattccaga tgacctatcg atcgtaggaa cagacgattc ttctttaagc catttaggga
    15721 ataaaaaatt gacaacattg acgcatccaa aagaaaagtt gggacaagat gccgcacggt
    15781 ggatcgtcac aacgattgaa aaaggacagg cacagccaaa tattctctat gaacctgtac
    15841 tagttgaacg ggaaacagtg aaagaaaacg aaaaataaga gacgggtttt taccgcctct
    15901 tattgctcct acggttactc gtcgcaatgt ttgtgagagc ttttgtcatc gctcaaggct
    15961 gaacgtttct ttcaccacct ctccactaaa cggtgttctc ctaccaactg gtcacaagtc
    16021 acaatattca tcaaacatga gaagctgttt tttgaaattg ttccttgtga ctggaaacta
    16081 agcggttcct tcttatttct tgaagctgac cacttctgtc acaacctctt taagaatgat
    16141 ggatagaaat acgtttagga taagtttttt tagatgcact ttgttcgatt tcctctaaag
    16201 atttcccacg agtttctggt acgatctctt gaataaataa aacccctaaa aagcagatga
    16261 tcccaaaaat gccgaaaacg atttcttgag acatagaagc agtcatcaca ggaaaaagca
    16321 agccaactag gaaagaacca atccaattaa aagaagaggc taggccagat gctcgcccgc
    16381 ggacagctag tgggaatatt tcgcctacga ttacccatgt cagtggagcc catgtgcaag
    16441 aataaaatgc gacataaata cataaaaaca agagaatcaa caacgaattt gtatgagcat
    16501 ccagtacagt tcctaaaaca gctggtagga tgaaagaaag tcccatcacg ctaccgccta
    16561 ttttcaataa agttcgccga ttgaacttgt ctgcaataac caaaaagagc aaagaaccag
    16621 caactaaaat gattccttgg atgatcggcc acatcaatgc atcgctcgct gcatgcccag
    16681 tcgctttttc tacgatcagc ggaatatagt aaaaaatagc attagctcct tgaaattgct
    16741 ggaatactgc tacaccaata ccggcaatga ctaaggaacg atatttttct aaaaacaaag
    16801 tatgccaaga agttccggtt gtcttttctt gtttgacatt cagctggatt tgttcgaatt
    16861 cctgttcagc ttcttttggt gtccgaatat agttcaatac tctttttgcc tcttccaagc
    16921 ggccagattg cataagaaaa cgcggtgact ctggtagagc taaaacaccg aaaaataaaa
    16981 tcagcgcggg gacagcagcg agccctaaca tcacgcgcca agccatcgtt tcaggcaacc
    17041 cttttagcaa ataatctgca acataggaaa ggagcatgcc agaagcaatc atcacttgat
    17101 tgatccctga caatcttccc cttagtctag caggagccat ttcagacata taagcaggaa
    17161 ctagggcaga tgcagcgcca acagctaaac ctagaagaat ccttgctgca atcagaaaat
    17221 aagaaccatt gtgaggggca gatgcagaaa ggacagaacc agccataaaa ataagtgcag
    17281 aaagaagaat catttttcgt cgtccaagct tatcagaaag aagaccagat aaagagccgc
    17341 caaggatcgc acctaacatg accgaagacg tgatccatcc tatcaaagaa gctttacctg
    17401 ccaaacccca atcatgctgt aaaaaaggta aggcgccggt cataacacca atatcatatc
    17461 caaatagaat gccgccaaat gcgccaaaaa aataaataaa cgatgaggaa actttttttt
    17521 cttttttcat aagtattctc tccttctaga atttcgagaa ggcggttgat cagattttgt
    17581 aagcaatttc attttataac ttatgtacgt acaaaacaag tgttttttat tgtattcttg
    17641 taaataactg cttgacatgt acgtacaaaa gtatacaata atcattgtaa gcgctactaa
    17701 aaagacggtt tgatcaggtt tgacgagaag gagggagtta aatcattatg aaggaagtaa
    17761 atgcgcaaag tatgttagat gtcatagatg gtcgtacatc tttagggatt gagtttggat
    17821 caactaggat caaagctgta ttgattgatt catcttttac tcctattgcc gctggtagtt
    17881 atgagtggga aaacaaacta gaaaatggta tctggaccta ttcattagaa gatatttgga
    17941 aaggactgcg cgtcagttac cgaaaaatgg cagcagaagt attcgaaatc tatggcgaga
    18001 atcttacgac aatcgggtcg attgggttca gtgcaatgat gcacggttat ctagcatttg
    18061 atagcaagga acagttacta gtaccatttc gtacttggcg aaatacaatc actgaagaag
    18121 cagaacgaat attaacggaa aaatttcaat acaatattcc gcagagatgg agtatcgctc
    18181 atttgtatca atctattttg aataaagaag aacatgtgaa agaaattaat tttttgacaa
    18241 cgcttgcagg ttatgtccat tggcagctta ctggtgagaa agtactaggc gtaggagatg
    18301 catcaggcat gtttcctatc gatccttcaa cgaaaaccta ccatcaagaa atgaaaaata
    18361 tttttggtca gttaatcgaa aaagcgcacg tagcgattga cttggatcac attttgccta
    18421 aagttctagt agcaggagaa gaagctggca agttaagcga atcaggggct tatctgatcg
    18481 accctacggg aaatttgaga ccaggaatcc cggtttgtcc gccagaaggg gatgcaggaa
    18541 ccggtatggt cgctacgaac agtgtagcca aagggacagg gaacatttcg gctggtactt
    18601 ccgccttcgc tatgatcgta ttagaaaaag aactagaaaa tgtccatccg gaaatcgaca
    18661 tggtcactac accttcagga gaactagtag gcatggttca tacgaataac tgctcttcgg
    18721 atatcaatgc ttgggtcagt atttttgaag aatttaccaa ttctttgggt atggagataa
    18781 gcagagataa attattcacg gtattattca acaaagcgct agaaggcgat acggattgcg
    18841 ggggattact ttcttatggt tatttttccg gtgaaaacat tacaggagta aatgaaggaa
    18901 ggccattatt tgtacgtaca ccaggtagcc gttttgattt agcgaatttc atgcggatcc
    18961 atttagccag tgcttttggt gcaatgagaa tcggaatgga tatattgaaa tctgagaatg
    19021 tccaaattga tcgtttagtg gggcatgggg ggatttttaa aacaccagaa gttggacagc
    19081 ggattctagc atctgctatg gaggcaccag ttacagtgat ggacacagcc ggagaaggtg
    19141 gagcgtgggg aatcgcgtta ttagcagcct atatgatgga tcgacatggg aagtctttag
    19201 aaaacttctt ggcagaagac gtgtttggac aagaccaagg aatcaccatc acaccttcag
    19261 cagaagaaat agcaggctat cagatattta tgagacgtta ccgtgagggg atcgacattg
    19321 aaaaaacagc gattcatcaa ttgcaagaaa aaggagtgat agaaaatgct ggaacaactg
    19381 aaagaagaag tataccaagc aaatcttgac ttgcctaaac acggactcgt caaatataca
    19441 tggggaaatg tgagcgcttt tgatcctgaa acacgatatt tcgttatcaa acctagcgga
    19501 gtcagttatg aagagatgac agcagacgac atggtcgttg tggacttgga caatcatatc
    19561 attgaaggta aactcaatcc ctcttcggat acgcctactc atgctgtttt atatcgttca
    19621 tttcctcaaa tcggaggaat cgtccatact cactcgacat gggcgacgat ctgggcacaa
    19681 gcaggattgg atgttcctgc gatggggacg acgcatgcag atacttttta tggatcggta
    19741 ccttgtgctc gatttttgac acaacaggaa atcgacagcg ggtacgaata tgaaacagga
    19801 aaagttatca tagaaacatt caaagaacgc aaaattgatc ccttagcaat tccaggggta
    19861 cttttacacg gacacggtcc atttacttgg gggaaagatg cgcaaagtgc ggtaatgaac
    19921 gcagtcgtac tagatgaagt atgcaagatg aatttattta ccagacagct caattcattt
    19981 tcagaagagc tgcctcaacg gattttagat aaacattatt tacgaaaaca cggaaaaaat
    20041 gcgtattacg gacaaaacaa ataaaaggag tggctaatta tgttgaatat tggagaaaaa
    20101 gaattttggt ttgttgtagg atcacagcat ttatatgggg aagaagcact gcgagaagta
    20161 aagaaacatg cacaagaaat ggtcgatgaa ctgaatgaaa atggccaatt gccttatcca
    20221 atccgccttc aagaattagc agttactgct gatacgatca caaaaatcat gaaagaggtc
    20281 aattatcgag aagaagtagc aggtgtgatc acttggatgc atactttctc accagcaaaa
    20341 atgtggatcc gcgggacaaa gcttctacaa aaaccattgc ttcatttagc tacgcaatat
    20401 aacgaaagca ttccttggaa aacgatcgat atggatttca tgaatttaaa tcaatccgct
    20461 catggtgacc gcgaatatgg gttcatcaat gctcgattaa ataaacaaaa taaaatcgtc
    20521 gttggctact ggaaacgacc tgagatccaa aaagagattg ctgattggat ggatgttgca
    20581 gtagcctata atgaaagttt tggaataaaa gtggctcgtt ttggtgacaa catgagaaac
    20641 gtgggtgtca cagaaggaga caaagtggaa gcacagattc aatttggttg gactgttgat
    20701 tattttggta tcggagatct tgtacaagta atcgatcgcg tatcagacga agaagtcgat
    20761 caattatttg aagagtacaa ggaattgtat accttcgact atggtgatta tgaagaaaag
    20821 acatgggagg aacatgtaaa agtacaagct cagcaggaaa tcggtattcg ccgtttctta
    20881 gaagaaggag gctataacgc ttttacgact aattttgaag acctgtatgg aatgaaacaa
    20941 ctacctggac tagctgtaca acgtttgatg gcagaaggat acggttttgc tggggaaggc
    21001 gactggaaaa ctgcagcgat tgatcggttg ctgaaaatca tggctcgagg gaaagataca
    21061 ggattcatgg aagattatac ttacgagcta gctagtggcc aagaagcaat cttggaatcc
    21121 catatgatgg aagtagatcc gacgcttgct gcgacgaaac caagaatcgt tgtttctcct
    21181 ttatcaatgg gagatagaga agatccggcg cgtcttgtat ttgatgggaa agcaggagaa
    21241 ggggtagtcg tatcgatggc agatttcggt acgcattata aactgctgat caatgaagta
    21301 gaagcctttg aaccaacgac tgaagcacct aatttacctg ttgcacgtgt gttatggaaa
    21361 actaaaccga atttccatga aggtgtccat tcatggattc aggcaggtgg cggtcatcat
    21421 accgttgtct cgctaaatct aacaacagat caaattgaaa cgtgggcaaa attagttgag
    21481 ttggaaacag tggttattag ataaaaagag gttgtgaaaa agccacttag ttttaagaaa
    21541 taagaagaga ttgacgaaat ttgcttttca acttttttga aatttagtct tattatcaat
    21601 gtcagctaac ggaacaccat ttatcaagat atctcaccct caatattttg ctctcgaaaa
    21661 taaggcttat ggcagtaact tgtcatagcc ttatttttat cactcagcct actctgctct
    21721 aactgctctt tttgcttcta ggaagtcaaa tggtaaacag ttgtctaatt gcagaaattt
    21781 cgagatactt gttagtaagg agggggtttt atgtatcacg ttcatttgat tgatgctatt
    21841 ccagaacaag agatagaaaa gttacaaggg gaatatttca aaataataga tgatttgcca
    21901 gccgaaggaa tagttattcg tagtacgaaa gtgaaagatg aatggcttac accagatttg
    21961 ctagcaattt caagagctgg tgttggggtg aacacgatca acgttgaaaa agcttctgaa
    22021 aacgggacga tcgtcatgaa tacaccaggt gtaaatgcta atgcagtaaa agagcttgtt
    22081 ttgtgctgtt tgctcttatc ttcccgtccg ataatcgaag caagtcgtat ggtacaaaca
    22141 ctaacaggtc ctaatatttt agaacaggca gaaaacaaaa gaagtgctta tgtgggacga
    22201 gaattgcaag gcaaaaccat cggtcttctt ggtcttggag cgataggcac aaaagtagcg
    22261 ctaagttgct atagcttagg gatggatgtc ttgggttatt ctatccgcga tgcccagttg
    22321 gattatgttc ggcaagcaga tttagaaaca gttctttcta cctcggatta catcgtagtc
    22381 atgctgccgt tgacagaaga tacaaaagga ttgatcgatc aggcgaatat agaaaaaatg
    22441 aaaaaagatg ctgtacttat caatgtagga agaagtgaaa ttgttgataa atatgctgtt
    22501 atgcaagcat tggaaaaaca acatctggct aaatacttga ctgattttcc agaagaagag
    22561 ttcttagaaa atgaccgtat acttatgttg cctcatctag gggatcgact caggaagctt
    22621 tggcagacag cggacgtttg gcagttgaag ccttaaaaga ttatcttttg ttcggtacgg
    22681 ttagagaagc ggtcaattat ccatccgcac gaatgttttt ccaagcgccg tttcgattca
    22741 ctatatttta tcaaaagaaa accaatatat tagcagagat attttctctg ctgaatgaga
    22801 acgagctagc gatcgcagat atatcgcgta accataagaa tgagcatgtc tacatattga
    22861 ttgacataga cagtactgat tttgaacagc tcaatcaagt gaaacaacag ctagaaaaaa
    22921 tttctggagt acgaaaggtc cgcttattaa agaaaccaga aaggccataa agaaagagaa
    22981 tatttggatt tcattaattc ttctttccgt tctttacttt cgaatttcgt acaactaaaa
    23041 taagagaagc aacattcttt tattgataga aatgaaggag caagcatttg aaaagatata
    23101 taagcagact ttcgaccaaa cagaaaaaga ttttaaaagg aacagcagca atcatcagta
    23161 tcttttttct tgttgttttt agtaatttat ttttgcagtg gtgtcaaaat gatctatccg
    23221 tagaccttgc gttgaaattt gctttttctt ggcatacgga aaaattcttc ttagcctgtc
    23281 tcgtcttgct gattattctt atttttctga ttgcactagc aggatctgtc cctttaggtt
    23341 cccttacgta tgtcgtagcc atcggtgtat taggttttgc aaattatatg aaaatgagtt
    23401 atcggcaaga accgatttat ccagacgatt tgaagatgat cactgaaatc ggattgctga
    23461 aagatatgac cggcactatg ctgtttacag tgatcttagc tgccgcagga accgtgatag
    23521 gattgttttg ctggtacatg tttcgaagcc tgaaaaaagg aaggcgattc caattgatcc
    23581 gattaacgac actgcttgtg gcaattggac tgctagggta tatcagtaat ttcaacaatc
    23641 ccgataattt gttgcgaaaa gcatataata aaacggcact atggatacct tacagccaga
    23701 aaatgaacta ctataacact ggttttatcg gtgggttttt gtacaatctg aaggtagaac
    23761 caatggatga acctgaagga tattcgaaag aaaagatcaa agaaatcact gaaaaatacc
    23821 agaagctagc cgatgaaaaa aataaaactg tagaggaaga gtcgccgaac atcgtgttcg
    23881 tgatgagtga aagtttctca gatcctagtc gattaaacgg cgtagaagta tctggtgagc
    23941 cattggcaga ttattatgaa gtagctgatc aaacatatag tggcaatatg ctttctcaaa
    24001 actatggcgg tggaacagca aatatcgaat ttgaagcatt gactggtttt tctatggcgt
    24061 tatttaatgc gcaactcacc accccttaca cgatgcttgt accaaaaatg gatcagcttc
    24121 cttctatcgt ttcagcgtta aacgcacaat cataccagac gacagcgatc catccataca
    24181 atacttcgat gtataaaaga gaggatgttt atcagacatt aggatttgac cagttcatca
    24241 gcgagcgtac gatgacctat acggatacga tagagaataa tccatatatt tctgatgagt
    24301 cagcttacaa agaagtaatg acacttctca aggaagaaaa aacacctcag ttcattcatt
    24361 tagtcacgat gcagacacat atgccctacg atggaaagta tgataatcta gactatcctg
    24421 caaaaacttc agatggaagt gggacacttg acttagagaa ttatctgcaa gatatttcct
    24481 atagcagcac ggcactgaag cagttcacgg aagaattgaa gaatctttct agaaggactt
    24541 tagttgtttt ttggggggat catcttccag ggatctattc agatacgatc caagcaaaaa
    24601 atgacaaaca aacattgcat gaaacacaat ttcttatgtt cgacagtaaa ggagaattag
    24661 aaaagcaaac gactcacgat gcaatcacca gtccgtttta ttttgcagcg aatctaatgg
    24721 aacagacgaa tcagacaaca aatggctttt atcagctgct tttatcatta gaacaggaac
    24781 ttccggcgtt tgaacgagaa ctgtactatc aaaacggaca gtggtataaa gaagcgcaat
    24841 tcaatcgctc gcaacaagag atttacgacg aatatcaatt gatccaatat gacattgtcg
    24901 cagggaaaca atatagttta gcagatggtt tctttgagca cgaataagaa tggcgagctg
    24961 tatagaaacc attaaatttt ttcaagtact tgtcatttga ggataaaaac aagtagaata
    25021 aataataaca acaatggaga aagtaggaaa acgaacatgt taccgaatat tggctattat
    25081 gtgcagtcca tcgatcaaat cgtcaaggaa acagaagcaa tcggagaagg aatgcatccg
    25141 aaatatgaag tgatcagaaa cgcaattgat gaaggaaaaa caagtgaact ggacaaatcg
    25201 actcttgaag agatcatcct gttatttgaa gaaggaacaa aaaaatatcg ggcaatgctt
    25261 atgaaagtca gtactttgcg tccaccagca aaagcaatgg gcattcataa aaattttgaa
    25321 aaagcttatc tttcatatgt agcaggctgt gaagaaatga tccaaagtat cgatcttgaa
    25381 tcaggggtag atgaagatct attcaataca tctgaagcaa aacaagacca agcgacagac
    25441 caattgacag ctgccatcca aaaaatgact aatttgttgt tgaaaaaata aaagaaatct
    25501 ttgataacaa taaaaactag agttatctaa tataaatgta atattacctt tatgatagag
    25561 tgatcaaaat cctttatgat aatgacatac ctataaccaa caacaacctt gtacttactc
    25621 aaaatcgagt aaatactcct tttccgtcga gtctaaccat ctcggcggtt ttttatttgt
    25681 agaaaagaac atcaggatag acggaaaagt cttgtcctga tgttcttttc agatagtatg
    25741 gaaagctgat tagttttcta cattctgtac atattgttca aagtagttct gtaaatcggc
    25801 tttgagtttc tgatattcct cttcgctata ctctttatct tcgattttcc catcaaagaa
    25861 aggaatattt agttcttccc gtagtctgtc taatacgtct tgctgattca tttcattctc
    25921 cttttcttac taacgatcaa aataaacaca gacacattct ggtacataga catctttttt
    25981 cagtaattcc agtaatcctg tttcaggatg acggcgatat aatgtcagat tatcggtgtt
    26041 ctgattggca cacacgacaa aattattgct tggatctagt gcgaaatcgc ggggaaaatc
    26101 cccttctgtt gagatgattt gcttgttttg aatgtgggaa ccatcttcaa aaactgcgaa
    26161 tacagcaatc gagttatgtc ctcggttaga agcataaagg aacttgccat catttgaaat
    26221 gcggatagct gcccctccat tttcaccgtc gaattcttct ggcaaggtcg atattttctg
    26281 taattcttca aaagcacctg tttctttatt ataagaaagc acgcggactg tgctgtctaa
    26341 ttcaccaaat aaataagcga attgtccatt tggatgaaag acaagatggc gagggcctgt
    26401 tcctggctct gcagtgaagg tagttacaag attcaatttg ccgttttcgg acacatcata
    26461 agtatacaca cgatctgtac caagatcaca tacgactaat cgttggtcag gagtcagatc
    26521 cgtatagtgg acatgcgcat gatcttggtt tttatgagga cctgtcgctt cttgatggaa
    26581 tattgaatct gctgctttca gttcgccatc tcctaatata tggtagacat ttacttcacc
    26641 tttatgatag ttcgccccgt atagtaattg acgtgcttca tcaaccgcta cataacacaa
    26701 aggagcgcca gactctgtta ccgcattcaa aaaatgaaaa tccttatcgt acgcaccggc
    26761 accacctaat ccatccacac tagtaactgt gtaaagattg ccagctttac tttttgctac
    26821 ataggtaggg ctattttctt caacagctaa tgaaaggttg gctaattctt ctttttctgt
    26881 atcaaggtcg atcgtataga ttcctttact ttcgcgacgc gtataggttc ctaaaattat
    26941 cttttcgatc atatgagctg attcctccta attgtaatga aatagttttc gtttttattc
    27001 tatcacataa ttattccaca cggctaaaga atgagaatgt gttatactgt ttttgaaagc
    27061 aattaaagga gagagtgcaa tgattcaggc aaagattaca gaaattggga cagaagcaat
    27121 aaacaaggaa gaacccatgc tgattttgtt cgataaaaca gcaacagctg ccttacgaaa
    27181 atattcggtg attcaagaag tatcaaaaga cgaacctttt tctttgaaag caggcggaac
    27241 aattgctttt gatgatcagg tatatacgat cgattatgtc ggtccgatgg ccaatgggaa
    27301 tttaaagaca gtagctcacg tatcattgat ttttgatgct tgtccagaag aaggacaaat
    27361 tgcaaatgga atctatcttc atccgcatag actgcctgct attggtttgg gaagccaaat
    27421 tttttataac taacaggagg aattatggaa ggaaaaaata agaagaacag accggtatcg
    27481 tggttttggc gctggttttt aaataatcaa gtagtcacag ccttacttgt tgtattgtta
    27541 atattgctga tcgtcttttt atttacaaaa gtttcatatt tgtttgcacc aatctggcaa
    27601 tttttagcaa tcgtaggtct gcctatcatt ttagcaggaa tcttgtatta cttgatgaat
    27661 ccggtagttg attactttga aaagcgaaaa gtaccaaggc tttacagtat cattgggctg
    27721 tttgttttgg ttgttgcact aatcgcatgg gggagcgtag tgatcatccc taagattcag
    27781 gaacaaacag tgagcttcat cagtaacttc cctcaatatg tagatacgat tgataataaa
    27841 ttaaccgaga ttttgcggga cccgctgttc aaccaatttc gcgaacagtt ggaaacagca
    27901 ggtgataagt tcatgaattc ggcaggcgat atgatccaag atatatctaa gagtacagtt
    27961 cagagtcttg gaagttttgt cggcgcggta gcgactattt tagtggctct gctgacgatg
    28021 ccgtttatct tattttatct attgaaagac ggtaaacaat tagcacctta ttttgtcaaa
    28081 tttttaccga cacgcatgca aaaaccgacg ttgaatgtgc tgagtgaaat gaatagtcaa
    28141 gtttcttctt atataagagg acaactaact gtggcttttg ctgtggcaat catgtttatg
    28201 attggttttg cggtcattgg tttagagtat gctgtcacgt tggggattat tgcgggtttt
    28261 ttaaacctga tcccttactt aggatcattt ttagcgatga ttccggctat ctttttagcc
    28321 attgtagcag gtccattcat gatcatcaaa gtactgatcg tttttgcagt agagcagaca
    28381 atagaaggac gcttgatctc tccattagta ctagggaatc agttagcaat ccatccagta
    28441 acgattttat tagttctgct gacttctggg aaattgttcg gaatcatcgg tgtgatcttg
    28501 gggattcctg tttatgcagc agctaaagta gtgattactc atgtttttga atggtacaca
    28561 acaatctctt ctctttatga agaaaatccg ttgaaaaagc agaaagaata acaaaaagag
    28621 acagatggga aaatatccaa ggcacaaatg ctgctttgaa gattttttcc tctgtctctt
    28681 ttgtatgcat ttatctcaat ttttaaaatg atttacctaa agcagcagct ttatctaatg
    28741 cattttgcat caactcatct gcacggtcag ggtcataatc gattccttcg atgaatagct
    28801 gatctacttg atcgatgcct acaaagttta aaataccttt tacatattga gaggcaaagt
    28861 cttgtccgtt gtatacaccg ccattagatt ggatgtgcag tgctttttta cctgtcacta
    28921 atccttctgg cccgttttct gtatagcgga acgtttttcc agctacattg acggtatcga
    28981 accaagcttt taattttgtt gggatattta gattccataa ggcgtttgcg ataacgattt
    29041 tatcagcagc aaggaactga tcagtcaatt cattgaaacg agcaattttt tgttgttgac
    29101 tttctgataa ggaagtgaat tctgcaccag cacgtaatgc ttcccaacct gataataatt
    29161 cttcatcgat ttccggaata tcatcgcgat aaagttccaa aacagtcact tcatcatctg
    29221 gatgattttc tttataactt gttaaaaaag acgtcaatgc cttaactgtg cgcgattctt
    29281 ctgcagttag gggatgacct ttgactacta aaacagatgc catatgctta ccttctcctt
    29341 ttattgctat gattgttata actatgacta acaaaatcag tttactcgaa aaagaaaagg
    29401 ctatcaagtc atattgataa ggttgaagaa taaataacta aaaaaagaca gggtataaaa
    29461 gaggctgtga cacgattttt tgtcacagcc tcttttgaat ctaacaagca atattcaagc
    29521 aacactcgac tgaagagctt ccatcccacc aatgatataa acagcacgaa aaccattagc
    29581 agtaagttga tcggctaatg cttctgatcg cctgcctgta tagcttagaa tataataaag
    29641 tttctttgga tctagatttt ttaattgtct tggcaagata gcagcgggga tagaaagcga
    29701 gtggcttaag tggttttttg tgtaatctaa ggtatttctt gtatctaaaa cgatgctgtc
    29761 agcatcgaga ggcaatttat tgaagtcttg gacactaatt gattgggcca tttatctcat
    29821 cctttgttta gtatgtggct attttaatgg gcaactctca ttaattcaaa tggaaggatt
    29881 cctcttatca cttttttaat gtatcggatg ataatgaaaa aaactatgca aatgtcttgc
    29941 aaaacaggta ggaggtgtta taatgacata gcgtcatatg acaatgtgtc atcttgaaag
    30001 aaaggagaat ccatatgccg acacagacat ttttccattt gcctaaagaa aaacaaaaaa
    30061 gactaatcga ggctgcgaga atcgagtttt ctagagtacc cttaaaagaa gcttcgattg
    30121 ctaatatcgt caaattagcg gatatcccta gaggcagttt ctatcagtat tttgaagata
    30181 aagaggatct ttatttttat tactttgaga caatgagacg tgacagctct aaagatatgt
    30241 tacaactaat gaaagaatcc gatggtgatc tattcaaagg attcgaatcc tatttttcta
    30301 aaatgattta tgagattttg gaaggcgaga atgcttcttt ttatcgtaac ttgtttatga
    30361 acatggatta ccgtgcctca agaaaagtcg ctcctcatac gagtcgccaa aaaacgcctc
    30421 acgatccaca taaagaacac aaacaagatg caaaagagtt gcttgaattg atcgatcgga
    30481 cgaagctgaa agtaaaaaat gatcatgaat tggaattact gatccaatta gtgatgaata
    30541 ctgtatttac aacaattgcg cattcgtatc gcttattttc aacatctgaa gattactcag
    30601 tagagcaagc aatcagtgaa ttcaatttga aactttcttg gctgaaaaac ggagtatata
    30661 aatagaaaga agggaactag atgctaaaaa tcgtaaaacg gatctccttg acttcagcaa
    30721 ttgcagctgc agttttcatg gtgatccaag tactggcaga tttatatttg ccgacactta
    30781 cctccaatat tattgataaa ggggtagcgc aaggagatgt cgattatatt tggcatacag
    30841 gttttgtgat gatcggcttt tctttgatca gtatcttagc tgctatcgca aatacatttt
    30901 ttgctactcg tgaatcacag aaactgggta agcaattgcg tacggatgtc tataaaaaat
    30961 cagagagtct gacaaaagat gcgtttgata aatatggaac ggcctctttg atcacaagaa
    31021 cgaccaatga tgtgactcaa atccaaatgg ttacacaaat gtttttacgt atgatgatca
    31081 atgcaccaat cacgttgatt ggcgcgagca tacttgctta tcaaaaagat catcagttga
    31141 caaaaatctt cttagttgtc atcccagtaa tgattatttt gatcggcgga atcatgtact
    31201 ttgctgttcc attattcaaa agtatgcaaa agaaaactga ccgtttaaac ctggtatttc
    31261 gagaaggatt gacaggggtc agagtgattc gagcattcga taagacacgc tttgaagaaa
    31321 atcgttttga tttagcgaat aaagattata caaatacggc aatcaaagtt aatacgatcg
    31381 tcgcattgat gatgccaatg atgacattga tcatgagcgg tacaaacgta gcaatcactt
    31441 ggtttggtgg acactacata gcagatatga ctttggaagt cggtaatctg attgcattta
    31501 tgacttatgc gatgcagatc ttgatcagct ttatgatgct ttccgctatt tttatcatgg
    31561 tgcctcgtgc acaagcttca gctgaccgta ttaatgaagt gttggatgaa aaaatcggta
    31621 tccatgaccc tgaaaatcct aaaacagttt cttttgctgg aaaaaacgca acattggcct
    31681 tcaatcatgt caattatcga tatcatggag cagaaaaact tgcattggaa gatattgatt
    31741 tccaagccaa gtcgggcgag atcgtcgcaa tcataggggg gactggttca ggtaaaacga
    31801 ccttagtcaa tttgatccct cgattatatg acatcgaatc tggttcgatc caaataaatg
    31861 gaacggacat tagtgacatg actcaatata atttgcgaga attaatgggc tttgttcctc
    31921 aaaaagcagt tctgtcctct ggaacgatcc gagacaatat gcagtatggt aagccggatg
    31981 caacagatga aatgatttgg aaagcattag aaatcgcaca agctaaagat tttgtttcag
    32041 agatggaaga tggcttagat agccatgtgg aacaaggagg aggaaacttc tctggtggac
    32101 aaagacagcg tctggcaatt gcacgggcat tagtcaaaac agcagatatc tatgtgtttg
    32161 atgattcctt ctcagcgttg gatttcaaaa cggatgccaa tcttcgccaa gcattgaaaa
    32221 cgaatatgaa agaaagtatc accgttttag ttgctcaacg agtaagtaca gtaatggatg
    32281 cagatatgat ccttgtgcta gatgaaggaa aactggttgg caaaggaaca catgaagaac
    32341 tattagcaac aaacgaaact tatcaagaaa tcgttcattc acaactaaga gaggaggact
    32401 tagcatgagt aaaacaaaca cacgtccctc tcatggacca gcaggcggtc caggacatgg
    32461 tggacctggc agaaatctcg gtgtaaaagg tgaaaagcct aaaaatttct ggggaacggt
    32521 acgccgattg tttggctata tgtctaaacg tatggttgcg atcatcgctg tatttgtact
    32581 agcaatagca gccgtgatct tccaaatcca aacaccaaag gtcttgggga aagcaaccac
    32641 agaaatattc aaaggtgtaa tggctggcgc tcaacagatg caacaaggcc aacaagtaac
    32701 aaaatttccg atagactttg ataaaatcgc gcagatcatt gcgacagtaa tcgtgatgta
    32761 tttgatttct gctgtattca actttttaca gcaatttatt atgacacgtg tttcccaacg
    32821 aacagtttat gaattacgtc atgatttgga agccaaaatg aatcgtgtac cgatttccta
    32881 ttatgataca catacaaatg gggatatcat gtctcgagca atcaatgata tggataacat
    32941 cgcaagcacg ctgcagcaaa atttgactca atttgtaact agtgcagtca cattgattgg
    33001 ggtcatcgtg atgatgcttt caatcagctg gcaattgacg ttagtcgcgt tattgatgat
    33061 ccctttaagc ttgatcatcg tgatgatcgt agcgccgaaa tcacaagtgt ttttcgcaga
    33121 tcagcaaaaa agtttaggtt tattgaataa ccaagtagaa gaaacatatg gtggtcatac
    33181 cattgttaaa acattcaatc atactgaaaa agaccaggaa gtatttgaaa aagaaaatca
    33241 gaatctgtat gccgctggtt ggaaagctca atttatttcg gcaatcatca tgccgttgat
    33301 gaatttcgtt aaaaatctag gctatgtgtt cgtcgctgta ctaggcggag taaaagttgc
    33361 aaatgggaac atgacattag gggatgttca agcgttcttg caatatacaa cacagttctc
    33421 tcaaccaatc actcaacttg caagtttgat gaacactatc caatcaacag ttgcttcagc
    33481 cgaacgggta tttgaagtgt tggatgaaga agagatgtcc aatgaaaaat ctggccttcc
    33541 agaagaaaaa gatacaccat acaaagttcg ttttgaaaat gttcaattcg gctacacacc
    33601 agataacttg ctaatgactg attttaactt ggatgtcaaa cctggtgaga tggtcgctat
    33661 tgtaggtccg actggtgcag ggaagacgac actgatcaac cttcttgaac ggttctacga
    33721 tgtttctgga ggtagtattc gttacgatgg tgtagatacc cgtgatcttt cacgtgatga
    33781 attgcgcgca cagttttcaa tggtactgca agatacttgg ctgtttacag gaagtatcta
    33841 tgacaatatc aaatatggga acgacgatgc aacagacgaa caaatcattg aggctgctaa
    33901 agctgctcac gtagacgatt ttgttcgtaa attgccagaa ggatataaca ctgtattaaa
    33961 cgaggatgca agcaatattt cgcaagggca gcgtcagcta atcacaattg cgcgggcatt
    34021 cttagcaaat ccagatgtcc tgatccttga tgaagcaaca tctagtgtag atacacgaac
    34081 agaaatactg attcaaaaag cgatgaaccg cctattggaa aaccggacaa gtttcgttgt
    34141 tgctcaccgc ttgtctacaa tacgcgatgc agacaacatt attgtgatga atcatggttc
    34201 gatcgtagaa acaggaaacc acgatacatt gatggaaaaa gacggattct atgcagatct
    34261 ttataacagt cagttttctg aagaagaagc ttcatagaat agtacaaatt tattattttt
    34321 tttaggggct gtgacaaaag tcgtgtcaca gtcccttttt ccgaataaac ggtgttcaat
    34381 cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat gagaagctat ttttccgttt
    34441 tgtatctcta atactcaggt caaaccgctg attttacaac ctcttcctta aacggtgttc
    34501 tcctgccaac tcctcggtca caagtcacaa tagtcaacaa acctgagaag ctgttttttg
    34561 aaattgctcc ttgtgactcg gagttaaacg gcaggctcac aacctcttgg taaacggtgt
    34621 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa agccattttc
    34681 acaaaatttt tcttactact cgaaactaag cgactttttc acaacctctt ccttaaacgg
    34741 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat
    34801 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
    34861 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
    34921 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
    34981 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
    35041 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
    35101 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
    35161 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
    35221 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
    35281 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
    35341 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
    35401 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
    35461 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
    35521 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
    35581 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
    35641 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
    35701 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
    35761 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
    35821 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
    35881 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
    35941 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
    36001 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
    36061 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
    36121 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
    36181 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
    36241 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
    36301 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
    36361 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
    36421 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
    36481 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
    36541 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
    36601 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
    36661 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
    36721 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
    36781 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
    36841 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
    36901 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
    36961 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
    37021 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
    37081 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
    37141 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
    37201 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
    37261 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
    37321 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
    37381 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
    37441 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
    37501 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
    37561 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
    37621 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
    37681 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
    37741 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
    37801 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
    37861 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
    37921 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
    37981 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
    38041 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
    38101 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
    38161 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
    38221 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
    38281 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
    38341 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
    38401 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
    38461 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
    38521 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
    38581 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
    38641 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
    38701 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
    38761 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
    38821 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
    38881 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
    38941 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
    39001 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
    39061 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
    39121 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
    39181 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
    39241 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
    39301 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
    39361 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
    39421 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
    39481 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
    39541 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
    39601 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
    39661 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
    39721 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
    39781 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
    39841 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
    39901 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
    39961 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
    40021 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
    40081 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
    40141 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
    40201 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
    40261 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
    40321 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
    40381 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
    40441 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
    40501 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
    40561 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
    40621 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
    40681 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
    40741 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
    40801 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
    40861 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
    40921 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
    40981 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
    41041 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
    41101 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
    41161 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
    41221 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
    41281 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
    41341 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
    41401 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
    41461 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
    41521 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
    41581 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
    41641 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
    41701 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
    41761 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
    41821 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
    41881 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
    41941 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
    42001 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
    42061 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
    42121 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
    42181 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
    42241 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
    42301 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
    42361 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
    42421 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
    42481 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
    42541 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
    42601 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
    42661 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
    42721 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
    42781 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
    42841 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
    42901 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
    42961 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
    43021 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
    43081 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
    43141 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
    43201 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
    43261 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
    43321 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
    43381 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
    43441 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
    43501 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
    43561 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
    43621 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
    43681 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
    43741 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
    43801 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
    43861 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
    43921 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
    43981 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
    44041 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
    44101 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
    44161 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
    44221 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
    44281 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
    44341 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
    44401 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
    44461 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
    44521 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
    44581 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
    44641 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
    44701 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
    44761 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
    44821 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    44881 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    44941 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    45001 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    45061 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    45121 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    45181 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    45241 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    45301 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    45361 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    45421 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    45481 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    45541 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    45601 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    45661 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    45721 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    45781 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    45841 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    45901 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    45961 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    46021 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    46081 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    46141 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    46201 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    46261 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    46321 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    46381 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    46441 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    46501 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    46561 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    46621 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    46681 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    46741 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    46801 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    46861 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    46921 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    46981 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    47041 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    47101 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    47161 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    47221 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    47281 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    47341 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    47401 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    47461 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    47521 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    47581 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    47641 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    47701 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    47761 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    47821 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    47881 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    47941 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    48001 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    48061 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    48121 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    48181 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    48241 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    48301 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    48361 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    48421 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    48481 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    48541 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    48601 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    48661 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    48721 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    48781 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    48841 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    48901 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    48961 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    49021 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    49081 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    49141 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    49201 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    49261 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    49321 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    49381 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    49441 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    49501 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    49561 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    49621 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    49681 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    49741 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    49801 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    49861 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    49921 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    49981 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    50041 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    50101 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    50161 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    50221 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    50281 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    50341 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    50401 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    50461 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    50521 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    50581 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    50641 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    50701 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    50761 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    50821 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    50881 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    50941 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    51001 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    51061 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    51121 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    51181 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    51241 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    51301 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    51361 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    51421 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    51481 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    51541 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    51601 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    51661 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    51721 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    51781 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    51841 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    51901 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    51961 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    52021 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    52081 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    52141 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    52201 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    52261 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    52321 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    52381 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    52441 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    52501 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    52561 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    52621 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    52681 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    52741 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    52801 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    52861 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    52921 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    52981 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    53041 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    53101 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    53161 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    53221 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    53281 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    53341 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    53401 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    53461 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    53521 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    53581 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    53641 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    53701 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    53761 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    53821 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    53881 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    53941 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    54001 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    54061 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    54121 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    54181 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    54241 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    54301 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    54361 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    54421 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    54481 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    54541 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    54601 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    54661 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    54721 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    54781 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    54841 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    54901 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    54961 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    55021 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    55081 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    55141 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    55201 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    55261 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    55321 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    55381 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    55441 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    55501 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    55561 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    55621 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    55681 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    55741 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    55801 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    55861 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    55921 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    55981 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    56041 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    56101 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    56161 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    56221 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    56281 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    56341 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    56401 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    56461 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    56521 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    56581 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    56641 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    56701 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    56761 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    56821 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    56881 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    56941 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    57001 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    57061 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    57121 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    57181 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    57241 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    57301 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    57361 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    57421 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    57481 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    57541 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    57601 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    57661 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    57721 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    57781 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    57841 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    57901 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    57961 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    58021 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    58081 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    58141 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    58201 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    58261 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    58321 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    58381 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    58441 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    58501 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    58561 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    58621 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    58681 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    58741 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    58801 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    58861 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    58921 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    58981 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    59041 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    59101 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    59161 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    59221 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    59281 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    59341 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    59401 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    59461 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    59521 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    59581 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    59641 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    59701 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    59761 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    59821 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    59881 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    59941 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    60001 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    60061 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    60121 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    60181 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    60241 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    60301 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    60361 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    60421 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    60481 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    60541 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    60601 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    60661 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    60721 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    60781 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    60841 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    60901 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    60961 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    61021 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    61081 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    61141 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    61201 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    61261 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    61321 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    61381 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    61441 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    61501 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    61561 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    61621 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    61681 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    61741 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    61801 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    61861 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    61921 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    61981 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    62041 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    62101 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    62161 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    62221 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    62281 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    62341 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    62401 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    62461 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    62521 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    62581 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    62641 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    62701 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    62761 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    62821 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    62881 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    62941 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    63001 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    63061 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    63121 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    63181 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    63241 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    63301 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    63361 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    63421 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    63481 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    63541 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    63601 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    63661 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    63721 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    63781 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    63841 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    63901 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    63961 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    64021 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    64081 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    64141 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    64201 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    64261 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    64321 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    64381 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    64441 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    64501 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    64561 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    64621 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    64681 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag atgttccgag
    64741 cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa atcagtatca
    64801 aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag ggaacagtct
    64861 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
    64921 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
    64981 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
    65041 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
    65101 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
    65161 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
    65221 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
    65281 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
    65341 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
    65401 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc taataaaaat
    65461 cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa aatcgttgcc
    65521 ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat ctagatgtag
    65581 cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg aaatcgtgat
    65641 gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg ttttaaatcc
    65701 agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct tagaaaaagg
    65761 tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt tagcaatcag
    65821 gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa taggaaaatt
    65881 tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc atttataaaa
    65941 acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc aggctaggtt
    66001 catttatttg aacagagtct gtattttttt attctaaata aagaaacaga tgtttcttta
    66061 tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat aatgttaatt
    66121 aaaagtgtat tttatgaaac tatcgttatt tattaatgtt gcatttttgt aagttaaaaa
    66181 ttggaaatat agattgaaaa aatgaaagca ataagtaaaa taaaatgaat atatgcgaaa
    66241 aaataattta cattaggaaa aaccttgtaa aaacagtttt ttggcatatt tattttttat
    66301 aaatatttgt aaaataacta tcaatttttt agttaaaaga ttggaaatga gtgggggagg
    66361 aattgaagaa aggatttttg agagatgata tagtaggttt tatttttagt taaaataggg
    66421 gagatagaaa aaatgaaaaa gtgtagaaat attattttat caatgttatt tattattact
    66481 aatataacat cactgattcc tgttcatgtc tatgcggatg caggcagaga tatcagcagt
    66541 aatgtcactt cgttgaccgt ggacccaacg aatattacag atggaggaaa cataaaagta
    66601 aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag gtgattatct ttggatcaat
    66661 tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa aagaaatccc attaatgatt
    66721 gaaaataaaa atgttggaac tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat
    66781 gaaaatatta aaaacctaga ctcagtagaa ggttgggggc aatttgagat acaggccaga
    66841 aacgtaaccg ataccggtga agaaaatacg ggaccattca ccgtgacgag cggtgataaa
    66901 acagctactg taaatgtgac aaagccagct tctggttctt cttctagcgt tttttattat
    66961 aaaacaggag acatgctacc tgaagatact aagcacattc gatggttttt gaatattaac
    67021 aataacggca cttatgttga acaaccagtt aaaatcagtg atgaaatcca aagtggacaa
    67081 agattagatc caagtacgtt tgaaattaat caaatacact taggtgaaca gaaggtttat
    67141 agaggagaag agggaatcca acaattttta caagatttcc ccagcgctac atttaatttt
    67201 tcagtaactg ataattacat tgaaataact ataccaaaaa actttgtcaa tcttaggaaa
    67261 ataatggtga gttataagac gatcatagaa aacccagaac aaataaattt tgagaatcat
    67321 tcggaagctt ggtttaaaga atttaataaa cctgctgttg atggagaaag tttcaatcat
    67381 acagttaaaa atatttcagc ttctggtgga gtcaacggta cagttagagg cgaattgaag
    67441 attttcaaat atatcaatga cacagagatt ggtatcccaa atgtgacttt cgaattaaga
    67501 cgagcagatg aacagcctat tcaaggacag tctagtatcc tgttgacttc gaatgaacag
    67561 ggagaagcta gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga aaaagaagca
    67621 cccaattgga ttgattttga tccactaagt tcaaatgaac tcaaattctc cataaacgaa
    67681 aatgatacag agggagtcag tttacctatt tataacaaga aaaaggttac gaacatcaca
    67741 gcaactaaaa tttggaacgg tggaaccact ccaagaccga gtatttattt taaattattc
    67801 agagcaagta caaacaattg ggaaccagtt cctgatgcag aaacaaaacg attagacaat
    67861 ggaataactt cagtgacttg ggaagatatc caacagtatg atgatagtgg aaacgaatat
    67921 acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc aggttatcaa
    67981 aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc agtctcagga
    68041 caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac gatcacagta
    68101 aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa agatgattgg
    68161 cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat ctatactgta
    68221 acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat caaaaacagt
    68281 tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc gtggaaaaat
    68341 tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa attgcaaata
    68401 tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa gagagctgta
    68461 cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag taaaaaatct
    68521 agttttgctg taatcattgg tttattgata gtaactatct gtgctgggat attcttacaa
    68581 aaatataaaa aagtttagtt agaatataat attttttgtt aaatatgact gaaatcaaaa
    68641 gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct tcttttttcg
    68701 tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta gttttcagcg
    68761 ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata ttttgtgcta
    68821 aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat tacaaatttt
    68881 cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa aaatccataa
    68941 tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga tcaatagcag
    69001 catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat ggagtcactg
    69061 tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa gataagcgct
    69121 tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca ggcaagatga
    69181 caagatagat ttgttttcct tttcttgatt tcaataatag attttttact tgcggaccag
    69241 gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact ttttgaaaag
    69301 agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga ctcatcgcgc
    69361 atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga aaaataatca
    69421 tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt
    69481 ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac acatcttgga
    69541 gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt
    69601 gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca
    69661 cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc tgaacggcaa
    69721 cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc acgtataata
    69781 cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa gaagatggag
    69841 tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt gaaattattt
    69901 ttatttacct tgattggctc attaccatta gggattgttt tagcattagg attgtctttg
    69961 cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg gggaacaccg
    70021 ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat cgtttttgat
    70081 cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt tgcagagatc
    70141 tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca agttttgcaa
    70201 ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa gattgtttta
    70261 ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat ctatatatta
    70321 ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc gactttactg
    70381 cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac aatcgtaatg
    70441 aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag aaatatgatt
    70501 gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta
    70561 atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg taaaaccacg
    70621 cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta tttagacggt
    70681 gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa
    70741 gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc accaaaaatg
    70801 gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc ggcttcatta
    70861 gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca aaaacagcgg
    70921 gtagcgattg ccagagcatt ggctatgaat ccacgggtat tggcttatga tgaaccaaca
    70981 agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa
    71041 gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat
    71101 caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg aaaaaaacca
    71161 gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa
    71221 cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata ggtttagacg
    71281 atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga tttgatattg
    71341 atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag cccattgact
    71401 ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg aacggctatt
    71461 ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg gaaaacgatc
    71521 aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg aataataaga
    71581 gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa ccagaagtat
    71641 taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat gaagcattga
    71701 tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac gcagactatt
    71761 atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct tatgagagtg
    71821 aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag
    71881 ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa tggtttggcg
    71941 aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt gtcacagtcc
    72001 ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc tgaatggcag
    72061 gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta agccaaattt
    72121 atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa actaagcgac
    72181 tttttcacaa cctcctttac atatttttat tttgttgtgg aaggggtttc gtgttattct
    72241 aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac aaagaactta
    72301 cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag
    72361 cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg atacctggaa
    72421 agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt tataaagaag
    72481 atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta gcttggacac
    72541 ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt tatgagagat
    72601 tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat cattgggatc
    72661 ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc attgaagatt
    72721 tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat tactggatta
    72781 gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca catccgccag
    72841 gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta gcaaatgcat
    72901 ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca agctttgcgt
    72961 atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag
    73021 aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat ccgattgctg
    73081 ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc gatgcagaat
    73141 tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg gcgacgaatg
    73201 cattcaatcc attaaacgga gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt
    73261 catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac
    73321 gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg cgtcgtatca
    73381 caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag tatgacaagt
    73441 tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa agccacgtca
    73501 aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc acctggtctt
    73561 atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt gtatatgttg
    73621 atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt tattactggt
    73681 accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta aaacaagaag
    73741 gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt aagaaaaact
    73801 gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac tttagttccc
    73861 ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg cacaaaagaa
    73921 acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga
    73981 acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct tcggcttttt
    74041 aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg caggaaatac
    74101 ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca ttaagaatac
    74161 agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt atttcggtat
    74221 cttattattg ttacatgccc atttatttgg caaactccat actggggaac caaatatcat
    74281 ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag acaataacgt
    74341 tggtggtggg atgattggag caatccttta tcattttact tacttcttga ttgcacagcc
    74401 tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt tcagtaatct
    74461 ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac aggaattatt
    74521 ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca
    74581 gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc
    74641 tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg
    74701 gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg acagttttca
    74761 agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa aacagaccaa
    74821 tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat tagaatttga
    74881 gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt tgcttgattc
    74941 tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt
    75001 agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc
    75061 atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta aaattgtgaa
    75121 cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg aagcaccgat
    75181 tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta tggtttcctt
    75241 tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag tacctttagg
    75301 acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc acttactcgt
    75361 ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca caagtatatt
    75421 aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa tggttgaatt
    75481 gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc
    75541 agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa aattcgcggc
    75601 aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa acttggaaaa
    75661 cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat tagctgattt
    75721 gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc aaatggctcg
    75781 ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg ttatcacagg
    75841 aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg gtacagattc
    75901 tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag atatgctatt
    75961 cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt ctgaccacga
    76021 agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg aagagaaaat
    76081 gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag atgagctgtt
    76141 tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc ttctccaaag
    76201 aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg aagctcatgg
    76261 agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc aggaagaaac
    76321 atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa
    76381 cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg tttttttcca
    76441 tcttttctaa tacggtatga tgtcttgtct ttttagtttt tgtgagatta aagttctatt
    76501 cgatttattt gtgaagtatt gcattaagga aatgaatcat gtatagtatg tagcgaaact
    76561 ttgagaaaaa gagggaaaat actttggaaa aacagtccat ttatggcttg acgaatgaag
    76621 aattaatcaa ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg
    76681 aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat
    76741 tgatcgaaaa attatcggag cattttatca tcaatcctct aaaacagatg gtcgtccaag
    76801 aagcttcgga tggtacagta aaatatttgt ttgaactccc tgataaaaac atgattgaga
    76861 ctgtactgat gagacaagaa tatggcttgt cagtttgtgt gacgacacaa gtaggatgta
    76921 atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg
    76981 gagaaattgt tgcacagatc atgatggtcc aacattattt tgatgagcgc aatcttggcg
    77041 aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc atttgataat tacgacaatg
    77101 taatggactt tttgcatatc atcaatgatg ccaaaggatt ggcaattggt gctcggcaca
    77161 ttacagtttc cactagtgga ttagcacaca aaatcaagga attcgctaat aacggcttgc
    77221 aagtcaatct ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc
    77281 ggatcaaccg aagtttccca attgagaaat tgatggaagc tgtggatgag tatttagaga
    77341 aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat gatagaccag
    77401 agcatgcgca acaattagca gatctattaa aagataagaa aaaattagca tatgtcaact
    77461 tgattccata taatccagtc agtgaacatg atcaatattc tcgcagtcca aaagctgatg
    77521 tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac
    77581 atggtacaga tattgatgca gcttgcggac aattgcgaag caagcaaatg aaaaaaacag
    77641 ttgccaaata atcgtatcct actcgttttt tcaattacca taaaaggagc agttttctta
    77701 attaaggaac tgttcaactt aaataagaga aaagattcta tagcgaaagc tataaagctt
    77761 tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat gacagcctgc
    77821 ttgatggggg ttttagcaat tatttaactg attttcttat atccttgctg gctttcttgt
    77881 gtagcatgat aagtttctac gtcaagaatg gcctgtttaa atttttctcg ggtgatttca
    77941 taaggcacat gtttaatatc atttgttgcc atggctttat ctaagattgc ttctaaatcg
    78001 gtcgtgacat tgagattcat gtcggttaac gatactggaa gttggttctt tttcataaac
    78061 acatagattt tatcaaacaa atcgaactgc tgatccatca tcaataaggt cagtacacca
    78121 taactgacag atgctccgtg aaggtatccc tccatttttt ctagaacagt cgtaccgtaa
    78181 tagaatgagt gtgccatagc agaattatag ttattgttaa ctaaaacaga aaccaatccg
    78241 gtagtaacaa tgatatccga aacgacttgt tcaattgcat atgagggacg atcgtcggca
    78301 ctatctttca atgcttgcgt accataagtc aagagacgat caatgcacat ttttccgatt
    78361 tggattccta atctatccgt gtagcctaga tcatcgccac gagcagaaaa agtagattcg
    78421 acttctttgg ataaagcatc gccaatacct gcccataagt atttatcagg tgcttcggca
    78481 ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc
    78541 attacgcctt cttcgttata gatgacacat accgcagtga cgggcgaaca gttcgaacca
    78601 atagtgggaa aggtgaagac tggtttagct agtttttcac caatgacttt tacagtatcg
    78661 caagcacggc caccaccgac tgcaaacagg acgtctgctt tttggacact ttcatgctgg
    78721 atcaactgtt caatatttcc ataagttgaa ttacccccgt accagagact ggcgacaacc
    78781 tctatttcct gtgtcaaagc tacttttatt ttgttttctg cctttgaaag tgcagtcttc
    78841 cctccaatca gcactacttt tgtcccatat tctttgacga ttccatttgc tttttcatac
    78901 gcatctgttc caatggtata atttggtaag aaaacacttt gactcatatt catttccttc
    78961 tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg atctgactgc ggctgtagat
    79021 ggagtagagg tgaacctaag acaggataag tgatgaataa ggtggcatga acaccttgaa
    79081 atcatcaata agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg acaataatga
    79141 atttttgttc ctcttagtat ttctaccgat tccctttgct tttttgtaat tatctaaaaa
    79201 ttctaaaaat taagattatt atatgaaaac aggttttatt tgtcaattca taattggttt
    79261 tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt cctccaattg
    79321 atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt ttttatttta
    79381 taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga tttttgagcg
    79441 agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta
    79501 tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac
    79561 tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga
    79621 tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg
    79681 gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct
    79741 cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt
    79801 ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa
    79861 gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg
    79921 aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg
    79981 caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt
    80041 ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa gaaggaattt
    80101 acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga agctactttt
    80161 ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt ttcgagtagt aagaaaaatt
    80221 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
    80281 ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag tttctttttg
    80341 tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat tatttaatgg
    80401 cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa ttccttgaaa
    80461 cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat tttttaataa
    80521 ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa attccaataa
    80581 aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat gcggccttgt
    80641 acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa aaagcggttc
    80701 agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta cagctgactt
    80761 atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg aaggaattta
    80821 ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg cagaagtcag
    80881 tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt caaatggtga
    80941 tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc cagctacagc
    81001 ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta cagctggaaa
    81061 gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag aaatcaaatt
    81121 gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt tcccacaaaa
    81181 acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata aagctgttta
    81241 taatggacca ttcacattag aagattttga tggaccagga acagatacag aatggactta
    81301 taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag
    81361 tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag cagatgacgt
    81421 gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata cttctgttaa
    81481 agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa
    81541 tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag taaaacaagt
    81601 actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg taaaaagtcc
    81661 aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg acaaagataa
    81721 agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt tagaatttga
    81781 tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc aaaactcaat
    81841 ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct ctgtacgtct
    81901 tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg ctgactatgc
    81961 agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc gtggtcgctg
    82021 gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg ctggcgatga
    82081 acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg atatgggtgt
    82141 cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt
    82201 tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag aataatcgtt
    82261 aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc gttctcatat
    82321 tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt agtattccgg
    82381 caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag gaaaattctg
    82441 gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga
    82501 aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat gaccataaga
    82561 atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt tttattaaaa
    82621 taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc atttgattgc
    82681 tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga agtcaaaagc
    82741 tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt ctttttcatg
    82801 atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt gctgccgggc
    82861 acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct aaataaacaa
    82921 gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa tttgcttcaa
    82981 ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt gttagctgga
    83041 agaatcggac catccttgca gttagggtta caggcaatta tctttggaac agtcctaggg
    83101 acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac atcttctaca
    83161 ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt attacaatat
    83221 atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt tgtgtacaca
    83281 atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg gtttatccgt
    83341 acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc aaaaggattg
    83401 agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc attgatgact
    83461 ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga aaatatcttt
    83521 gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga ttatcctacg
    83581 atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt gatcgtggat
    83641 ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg aggataaatg
    83701 gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga atttcttcca
    83761 ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt gaattttatc
    83821 caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat ggttttacta
    83881 ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga tccaactgcc
    83941 caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat cgatggattg
    84001 aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc aaacgtccct
    84061 tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt aagccgctta
    84121 tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact agatgtaacg
    84181 attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg
    84241 caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg
    84301 gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac aaactggata
    84361 ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga atatgtttta
    84421 gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat tttgccgaat
    84481 atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat tttcttcgaa
    84541 gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt aggaacgatg
    84601 ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat tccggcagta
    84661 actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg tgatgccttt
    84721 gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa gacttagaaa
    84781 tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat tttcatttga
    84841 ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca gtgacgacca
    84901 gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa atcctcttta
    84961 aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa
    85021 tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc attgggaagc
    85081 aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga caaaaagctg
    85141 cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc aacagttatc
    85201 cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt
    85261 atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact attcaagcac
    85321 agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt atctttatca
    85381 ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa
    85441 gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat ccctatactt
    85501 ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg tatgctattc
    85561 ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat ccaagaaatg
    85621 aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta tcaaaaacac
    85681 acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg ccagaagaaa
    85741 tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt caaggaggaa
    85801 ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt caatgtagga
    85861 aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga aggagaaaca
    85921 ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac gatcattcgc
    85981 ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac gaagatcaaa
    86041 gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca agatccatac
    86101 gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat tgatgttaat
    86161 ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt gagaactgtt
    86221 ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg gcaacgccag
    86281 cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg tgatgaacca
    86341 atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca agatctccaa
    86401 aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt taaacatatt
    86461 agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac aagtgatgaa
    86521 atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat tcctctacca
    86581 gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc agatgacgga
    86641 caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac tgagcaggaa
    86701 gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt tgctaactag
    86761 gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa gaacaaaaac
    86821 agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg ctgaaaaagc
    86881 ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc gctacattaa
    86941 aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat gcacagctta
    87001 atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca aagcttaaaa
    87061 cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg atctatctgg
    87121 aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat cagcatcggc
    87181 cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa gaattttatg
    87241 tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa tggatcggga
    87301 tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta actttaccaa
    87361 aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc
    87421 gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag aaacaaaaga
    87481 gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat cttgaacaat
    87541 tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt ccaaaagaag
    87601 aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa ttgcgttata
    87661 agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca gaagaaagca
    87721 attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt atcaagggat
    87781 aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa aagtcttaat
    87841 gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg acaaaccaat
    87901 aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt ttttattcag
    87961 aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa gagttgtttt
    88021 tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat tcatggtgcc
    88081 ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt tcaccgatct
    88141 catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt ttataatcga
    88201 tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct ttacctaaaa
    88261 cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat gctttcaaga
    88321 tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca gtgactaaat
    88381 caagcgagat agccacttct ccgctagata aatggatctt gttactatga cgttctagtg
    88441 tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct ccaataaagg
    88501 ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta ctgaatgtat
    88561 gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt gtttctctta
    88621 agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat gcccatgaat
    88681 ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct tcttctttgt
    88741 tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct ggacgttcct
    88801 gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa tataattttt
    88861 cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta aaagtacttt
    88921 ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta
    88981 tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa agcagataaa
    89041 acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata acgatatata
    89101 cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa tgcccatgga
    89161 ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt gctaatgaaa
    89221 gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt ccagtccctt
    89281 actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa gccgtttttt
    89341 agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc ttttaaagga
    89401 tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa aaaagctaac
    89461 agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc gataaaggag
    89521 gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct attatccaag
    89581 cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa ttgctcgtta
    89641 taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac atggtcaaaa
    89701 aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc
    89761 ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat tcattcgcac
    89821 gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat tagaacaggg
    89881 agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag aattctttac
    89941 agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc
    90001 gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc gcatgagcaa
    90061 atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc agccagaatc
    90121 tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc ttgcagtttc
    90181 acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg tggtgtatgt
    90241 atggatcgat gcattatcca attatattac tgcactagga tatggctccg aagatgaaag
    90301 tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt
    90361 tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt
    90421 atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt
    90481 cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt attatttgct
    90541 tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg tttcccgtgt
    90601 caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg ctatgatcaa
    90661 caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat ttgatagtga
    90721 actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga
    90781 attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca aatacattga
    90841 tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt
    90901 catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc caatcatgac
    90961 agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa tgaatcttga
    91021 aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag gtacaccaat
    91081 cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg
    91141 tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac
    91201 gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt
    91261 catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac tagatgctgg
    91321 agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg atccaagcga
    91381 attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca
    91441 aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac aagtaatcga
    91501 agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag ggggtatgac
    91561 actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt aaattccttc
    91621 ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa taaatcctag
    91681 aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa taggaatcgt
    91741 aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga ttcatctggt
    91801 actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta ttacatctat
    91861 atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt tatgttcaat
    91921 tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc atcggctttg
    91981 acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt atggtaacct
    92041 tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa
    92101 aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt tgtcaacatc
    92161 acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg attatcccaa
    92221 tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata gatcccgact
    92281 aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa
    92341 agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat cccccacaag
    92401 atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag caatattagt
    92461 ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa
    92521 tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata atctgctaat
    92581 ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa ggtaggttta
    92641 gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc actgaaagtg
    92701 gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat accaaatttc
    92761 aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat ctgtttggcg
    92821 cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg
    92881 ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg ggtggtttct
    92941 taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg gaaactggtg
    93001 gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta ttaggatggg
    93061 cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc tttgcgacac
    93121 aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca gtgatcaccg
    93181 ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct gtacaatcag
    93241 tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga ttattgaccc
    93301 caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg
    93361 aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg
    93421 gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca attattcttg
    93481 gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg aaaactttgc
    93541 cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac gtcatttttg
    93601 gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac ggtgcattga
    93661 acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa gacgagctac
    93721 ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat gttcctgcgg
    93781 ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac tttttaacag
    93841 acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt gtctttattt
    93901 taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat ccaatcgttc
    93961 cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg acgcaaacga
    94021 aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat tatcaaaaaa
    94081 agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt acgaactagg
    94141 tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat tttgattttt
    94201 gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga gataaacatg
    94261 atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat tccagaaacg
    94321 attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca
    94381 acgattgaaa agtctttgca attaagccat gactatccaa atatatacag tattatcggc
    94441 tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt acaagaacaa
    94501 ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca ttggatggaa
    94561 gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc aaaagaaatg
    94621 aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca aatcctaaaa
    94681 gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga ttatgaatgg
    94741 gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt cactttcaaa
    94801 aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa
    94861 acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga accaggctat
    94921 actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga agaagtggct
    94981 gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg ataagatgaa
    95041 gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc gggaagtagt
    95101 tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc tgatgcaaat
    95161 tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt tttcaggaga
    95221 aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt ttttgccgcg
    95281 cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc
    95341 tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg ctgtttctga
    95401 aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag caaaagaaaa
    95461 acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac aattggaaaa
    95521 acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga aaatagtgga
    95581 ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa gagattttat
    95641 tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact gaaccaaata
    95701 ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg
    95761 gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa gtcgtagcat
    95821 ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc tatgacaatg
    95881 tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt agagaaacat
    95941 ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt acgacaccga
    96001 tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc atgatgcaaa
    96061 aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt tcattatcga
    96121 ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa acagtatttg
    96181 tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat ataccggcag
    96241 tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt cagcaaagaa
    96301 gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag acaaaagcat
    96361 ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg gagactttgt
    96421 cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga
    96481 ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
    96541 aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca agcttttgaa
    96601 caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa ggaacaattt
    96661 caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg caagcttttg
    96721 accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc ctatttttgt
    96781 ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg atttagaaaa
    96841 gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat ttatgtgtca
    96901 agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta acgatttctg
    96961 ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc atgattccac
    97021 cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt tttacaccag
    97081 gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca acgccggtta
    97141 tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct tggtacttgc
    97201 aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc
    97261 aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta gtagtcagca
    97321 tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat ttttatacag
    97381 tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat aatattgctt
    97441 attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa
    97501 aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat tagacatgat
    97561 ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga tgagagtttt
    97621 ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg ggcaatttat
    97681 ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct gatgttgaag
    97741 gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt tgagatgaac
    97801 tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc ggaaacgaca
    97861 acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg
    97921 tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc aactaatact
    97981 ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat aacaccattt
    98041 ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc aacgaacata
    98101 ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa tccgtctttc
    98161 atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt aacatagatg
    98221 gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc tacacgtaat
    98281 ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc ccgaacgatt
    98341 gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg ttttcgtttc
    98401 aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt gatgtctgct
    98461 ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt catatgagga
    98521 tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc cacgtcattc
    98581 atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc cgcgatcatt
    98641 cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa ttcacggtct
    98701 ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata ttttttatat
    98761 acgtttttca gagccatttc taccataata cgttccacac tttctttttt agttttatta
    98821 actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga acaagaattt
    98881 tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata agaaataaaa
    98941 actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa
    99001 atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat
    99061 aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca gaggatcacg
    99121 gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg ggatcagcaa
    99181 attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg agacccgctg
    99241 gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga tgtctatgag
    99301 attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact ggaagccggc
    99361 ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc attttgatgg
    99421 ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa aagctaaaca
    99481 aactgataat atgcgatata atacgaacgt attggataat ataaacaata atattagttt
    99541 ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa
    99601 taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg cctcttggac
    99661 gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga tgtagcagct
    99721 cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga ttgctattat
    99781 gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg agaccccatt
    99841 tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt ttggtccttt
    99901 ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct tctcgaaatc
    99961 tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct acggattctt
   100021 tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta gtgtcacgct
   100081 gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg tgccattgtt
   100141 cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac cgattcaatc
   100201 aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa gaagtttagc
   100261 gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct tgtttttatt
   100321 gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga agcagctcat
   100381 catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg tccgcatttt
   100441 tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc attgctaaga
   100501 aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct tcatctaaga
   100561 tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg cgttgtccat
   100621 ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc aaacgttcta
   100681 caaattcata catataagag gcttttaggg catcattgat ttctgcgtca cttgcttccg
   100741 gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca
   100801 ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca tggtcatcga
   100861 ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca agtgtggatt
   100921 ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca gtaaaattga
   100981 aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca aaagagactt
   101041 ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc ttttcttctg
   101101 gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg
   101161 tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc gtaaagctgg
   101221 aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg ccaactaaac
   101281 caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc aaggaagaag
   101341 aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca aattgctgtt
   101401 gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt cgttcggtca
   101461 caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt tgtgaagcac
   101521 gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg accaatgtca
   101581 gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc actccagaaa
   101641 ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca tttgtaaatc
   101701 gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat cgattcagat
   101761 gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga ttgcttaaca
   101821 cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat agaataagta
   101881 aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg atactttgac
   101941 caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt atcacagcta
   102001 gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg
   102061 ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt cctgatattc
   102121 tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac cttgatccat
   102181 cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa taatcactgt
   102241 actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat aatccaacgc
   102301 acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt
   102361 cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt ttgtatctaa
   102421 accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag cttcccaaca
   102481 ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag tcccggtaaa
   102541 tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca gctgccaaga
   102601 acgaacatcc attccgtcca ttataactgt accggaactg acatcataga aacgcggaat
   102661 caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa ggactgtatt
   102721 tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag tataacggaa
   102781 gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag tactgacctc
   102841 agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc ctgaagcttc
   102901 ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa gcatttgatt
   102961 catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat ttactttgat
   103021 cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg tagcaggtga
   103081 taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat ttgtgctttt
   103141 atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac gaacaccgct
   103201 aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt tttggtagag
   103261 aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca ataatgctaa
   103321 gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc cgatacttaa
   103381 aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa gctgattgat
   103441 gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga
   103501 gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc cttgtgaagc
   103561 tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag acatagcgac
   103621 catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc cattatcgat
   103681 caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa
   103741 cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact gaatcatatg
   103801 attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac attgtagctt
   103861 aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt attatgaaga
   103921 agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa aacatgccag
   103981 tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa ttctgcataa
   104041 aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt taatttgttc
   104101 ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa atctaattga
   104161 ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt gggtactatc
   104221 ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga
   104281 tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt gatctgaaaa
   104341 taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg
   104401 aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta
   104461 ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca
   104521 catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt
   104581 ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt
   104641 cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa
   104701 gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc
   104761 aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt
   104821 attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa
   104881 taaaaattat aagggataag taggctgctg tctataataa gatatatttt tcaattcttt
   104941 attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt
   105001 tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac
   105061 atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca
   105121 cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc
   105181 gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga
   105241 atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag
   105301 acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca
   105361 aaacagctct ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca
   105421 caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag
   105481 aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt
   105541 tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa
   105601 aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa
   105661 caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg
   105721 gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga
   105781 ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg
   105841 cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag
   105901 aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa
   105961 gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa
   106021 aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa
   106081 aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc
   106141 aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta
   106201 aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg
   106261 aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta
   106321 gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac
   106381 aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact
   106441 atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa
   106501 tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac
   106561 gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt
   106621 tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga
   106681 tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat
   106741 tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg
   106801 cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag
   106861 tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc
   106921 ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa
   106981 aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag ccagaatggc
   107041 gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa
   107101 tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt
   107161 tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat
   107221 tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg
   107281 actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt
   107341 tggacactac tatgaatgct tcagagaagg aaatattcaa gcagcgcatc attcgtaatt
   107401 tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag
   107461 aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca
   107521 aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc
   107581 caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg
   107641 ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg
   107701 agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag
   107761 aattttgaac ataagcaagt tgttgccact
//
LOCUS       DAPBBG010000003        86304 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPBBG010000003 DAPBBG010000000
VERSION     DAPBBG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 86304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.003"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(683..973)
                     /locus_tag="RD477_000210"
     CDS             complement(683..973)
                     /locus_tag="RD477_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632605.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1181..1873)
                     /locus_tag="RD477_000211"
     CDS             complement(1181..1873)
                     /locus_tag="RD477_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDT7632606.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            2268..2891
                     /locus_tag="RD477_000212"
     CDS             2268..2891
                     /locus_tag="RD477_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7632607.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(2975..3697)
                     /locus_tag="RD477_000213"
     CDS             complement(2975..3697)
                     /locus_tag="RD477_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDT7632608.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(3687..4424)
                     /locus_tag="RD477_000214"
     CDS             complement(3687..4424)
                     /locus_tag="RD477_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632609.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(4436..5428)
                     /locus_tag="RD477_000215"
     CDS             complement(4436..5428)
                     /locus_tag="RD477_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7632610.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(5443..6186)
                     /locus_tag="RD477_000216"
     CDS             complement(5443..6186)
                     /locus_tag="RD477_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632611.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(6260..6364)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6427..7749)
                     /locus_tag="RD477_000217"
     CDS             complement(6427..7749)
                     /locus_tag="RD477_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7632612.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(7736..8701)
                     /locus_tag="RD477_000218"
     CDS             complement(7736..8701)
                     /locus_tag="RD477_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632613.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(8698..10236)
                     /locus_tag="RD477_000219"
     CDS             complement(8698..10236)
                     /locus_tag="RD477_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDT7632614.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(10652..10939)
                     /locus_tag="RD477_000220"
     CDS             complement(10652..10939)
                     /locus_tag="RD477_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632615.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            11073..11741
                     /locus_tag="RD477_000221"
     CDS             11073..11741
                     /locus_tag="RD477_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7632616.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(12032..12772)
                     /locus_tag="RD477_000222"
     CDS             complement(12032..12772)
                     /locus_tag="RD477_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632617.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(12902..13849)
                     /locus_tag="RD477_000223"
     CDS             complement(12902..13849)
                     /locus_tag="RD477_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7632618.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(13978..14742)
                     /locus_tag="RD477_000224"
     CDS             complement(13978..14742)
                     /locus_tag="RD477_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7632619.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(14746..15135)
                     /locus_tag="RD477_000225"
     CDS             complement(14746..15135)
                     /locus_tag="RD477_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632620.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            15270..15812
                     /locus_tag="RD477_000226"
     CDS             15270..15812
                     /locus_tag="RD477_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDT7632621.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGNIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            15960..16592
                     /locus_tag="RD477_000227"
     CDS             15960..16592
                     /locus_tag="RD477_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632622.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16726..16851)
                     /locus_tag="RD477_000228"
     CDS             complement(16726..16851)
                     /locus_tag="RD477_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7632623.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(16985..17878)
                     /locus_tag="RD477_000229"
     CDS             complement(16985..17878)
                     /locus_tag="RD477_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDT7632624.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(18060..18986)
                     /locus_tag="RD477_000230"
     CDS             complement(18060..18986)
                     /locus_tag="RD477_000230"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDT7632625.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(19071..19832)
                     /gene="pflA"
                     /locus_tag="RD477_000231"
     CDS             complement(19071..19832)
                     /gene="pflA"
                     /locus_tag="RD477_000231"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDT7632626.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(20076..22307)
                     /gene="pflB"
                     /locus_tag="RD477_000232"
     CDS             complement(20076..22307)
                     /gene="pflB"
                     /locus_tag="RD477_000232"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDT7632627.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(22584..25034)
                     /gene="parC"
                     /locus_tag="RD477_000233"
     CDS             complement(22584..25034)
                     /gene="parC"
                     /locus_tag="RD477_000233"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDT7632628.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(25046..27091)
                     /gene="parE"
                     /locus_tag="RD477_000234"
     CDS             complement(25046..27091)
                     /gene="parE"
                     /locus_tag="RD477_000234"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDT7632629.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(27257..27691)
                     /locus_tag="RD477_000235"
     CDS             complement(27257..27691)
                     /locus_tag="RD477_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDT7632630.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            27818..28456
                     /gene="plsY"
                     /locus_tag="RD477_000236"
     CDS             27818..28456
                     /gene="plsY"
                     /locus_tag="RD477_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDT7632631.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(28588..29463)
                     /locus_tag="RD477_000237"
     CDS             complement(28588..29463)
                     /locus_tag="RD477_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDT7632632.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(29545..30336)
                     /gene="codY"
                     /locus_tag="RD477_000238"
     CDS             complement(29545..30336)
                     /gene="codY"
                     /locus_tag="RD477_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDT7632633.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(30354..31754)
                     /gene="hslU"
                     /locus_tag="RD477_000239"
     CDS             complement(30354..31754)
                     /gene="hslU"
                     /locus_tag="RD477_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDT7632634.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(31768..32316)
                     /gene="hslV"
                     /locus_tag="RD477_000240"
     CDS             complement(31768..32316)
                     /gene="hslV"
                     /locus_tag="RD477_000240"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDT7632635.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(32353..33258)
                     /gene="xerC"
                     /locus_tag="RD477_000241"
     CDS             complement(32353..33258)
                     /gene="xerC"
                     /locus_tag="RD477_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDT7632636.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(33456..34832)
                     /gene="trmFO"
                     /locus_tag="RD477_000242"
     CDS             complement(33456..34832)
                     /gene="trmFO"
                     /locus_tag="RD477_000242"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDT7632637.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(34801..36879)
                     /gene="topA"
                     /locus_tag="RD477_000243"
     CDS             complement(34801..36879)
                     /gene="topA"
                     /locus_tag="RD477_000243"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDT7632638.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(37001..37858)
                     /gene="dprA"
                     /locus_tag="RD477_000244"
     CDS             complement(37001..37858)
                     /gene="dprA"
                     /locus_tag="RD477_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDT7632639.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(37914..38681)
                     /locus_tag="RD477_000245"
     CDS             complement(37914..38681)
                     /locus_tag="RD477_000245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDT7632640.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(38674..39534)
                     /gene="ylqF"
                     /locus_tag="RD477_000246"
     CDS             complement(38674..39534)
                     /gene="ylqF"
                     /locus_tag="RD477_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDT7632641.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            39794..40192
                     /locus_tag="RD477_000247"
     CDS             39794..40192
                     /locus_tag="RD477_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7632642.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            40471..40971
                     /locus_tag="RD477_000248"
     CDS             40471..40971
                     /locus_tag="RD477_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDT7632643.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(41054..41605)
                     /gene="lepB"
                     /locus_tag="RD477_000249"
     CDS             complement(41054..41605)
                     /gene="lepB"
                     /locus_tag="RD477_000249"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7632644.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(41757..41984)
                     /locus_tag="RD477_000250"
     CDS             complement(41757..41984)
                     /locus_tag="RD477_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDT7632645.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(41981..42499)
                     /gene="msrA"
                     /locus_tag="RD477_000251"
     CDS             complement(41981..42499)
                     /gene="msrA"
                     /locus_tag="RD477_000251"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7632646.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(42519..43232)
                     /locus_tag="RD477_000252"
     CDS             complement(42519..43232)
                     /locus_tag="RD477_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDT7632647.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(43235..44131)
                     /locus_tag="RD477_000253"
     CDS             complement(43235..44131)
                     /locus_tag="RD477_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7632648.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(44289..45131)
                     /locus_tag="RD477_000254"
     CDS             complement(44289..45131)
                     /locus_tag="RD477_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7632649.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            45263..45916
                     /locus_tag="RD477_000255"
     CDS             45263..45916
                     /locus_tag="RD477_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDT7632650.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(45979..46506)
                     /locus_tag="RD477_000256"
     CDS             complement(45979..46506)
                     /locus_tag="RD477_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7632651.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(46526..47473)
                     /locus_tag="RD477_000257"
     CDS             complement(46526..47473)
                     /locus_tag="RD477_000257"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDT7632652.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(47485..49371)
                     /locus_tag="RD477_000258"
     CDS             complement(47485..49371)
                     /locus_tag="RD477_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7632653.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(49531..51609)
                     /locus_tag="RD477_000259"
     CDS             complement(49531..51609)
                     /locus_tag="RD477_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDT7632654.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(51622..51897)
                     /locus_tag="RD477_000260"
     CDS             complement(51622..51897)
                     /locus_tag="RD477_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632655.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(52259..52714)
                     /locus_tag="RD477_000261"
     CDS             complement(52259..52714)
                     /locus_tag="RD477_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632656.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(52868..54076)
                     /locus_tag="RD477_000262"
     CDS             complement(52868..54076)
                     /locus_tag="RD477_000262"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDT7632657.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(54188..54520)
                     /locus_tag="RD477_000263"
     CDS             complement(54188..54520)
                     /locus_tag="RD477_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDT7632658.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            54668..55543
                     /locus_tag="RD477_000264"
     CDS             54668..55543
                     /locus_tag="RD477_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7632659.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(55625..56206)
                     /locus_tag="RD477_000265"
     CDS             complement(55625..56206)
                     /locus_tag="RD477_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDT7632660.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(56212..57471)
                     /locus_tag="RD477_000266"
     CDS             complement(56212..57471)
                     /locus_tag="RD477_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDT7632661.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(57473..58519)
                     /locus_tag="RD477_000267"
     CDS             complement(57473..58519)
                     /locus_tag="RD477_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632662.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(58754..59029)
                     /locus_tag="RD477_000268"
     CDS             complement(58754..59029)
                     /locus_tag="RD477_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDT7632663.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(59272..60582)
                     /gene="der"
                     /locus_tag="RD477_000269"
     CDS             complement(59272..60582)
                     /gene="der"
                     /locus_tag="RD477_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDT7632664.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(60764..61993)
                     /gene="rpsA"
                     /locus_tag="RD477_000270"
     CDS             complement(60764..61993)
                     /gene="rpsA"
                     /locus_tag="RD477_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDT7632665.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(62128..62808)
                     /locus_tag="RD477_000271"
     CDS             complement(62128..62808)
                     /locus_tag="RD477_000271"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDT7632666.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(62857..63492)
                     /locus_tag="RD477_000272"
     CDS             complement(62857..63492)
                     /locus_tag="RD477_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7632667.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(63558..64961)
                     /locus_tag="RD477_000273"
     CDS             complement(63558..64961)
                     /locus_tag="RD477_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDT7632668.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(64948..65979)
                     /locus_tag="RD477_000274"
     CDS             complement(64948..65979)
                     /locus_tag="RD477_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7632669.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            66010..66231
                     /locus_tag="RD477_000275"
     CDS             66010..66231
                     /locus_tag="RD477_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDT7632670.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(66396..67133)
                     /locus_tag="RD477_000276"
     CDS             complement(66396..67133)
                     /locus_tag="RD477_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDT7632671.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(67286..68626)
                     /locus_tag="RD477_000277"
     CDS             complement(67286..68626)
                     /locus_tag="RD477_000277"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDT7632672.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(68863..70386)
                     /locus_tag="RD477_000278"
     CDS             complement(68863..70386)
                     /locus_tag="RD477_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDT7632673.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(70764..71375)
                     /locus_tag="RD477_000279"
     CDS             complement(70764..71375)
                     /locus_tag="RD477_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7632674.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(71498..71623)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(71705..72421)
                     /locus_tag="RD477_000280"
     CDS             complement(71705..72421)
                     /locus_tag="RD477_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDT7632675.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(72427..72996)
                     /gene="scpB"
                     /locus_tag="RD477_000281"
     CDS             complement(72427..72996)
                     /gene="scpB"
                     /locus_tag="RD477_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDT7632676.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(72980..73777)
                     /locus_tag="RD477_000282"
     CDS             complement(72980..73777)
                     /locus_tag="RD477_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDT7632677.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(73747..74118)
                     /locus_tag="RD477_000283"
     CDS             complement(73747..74118)
                     /locus_tag="RD477_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDT7632678.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(74137..75024)
                     /gene="xerD"
                     /locus_tag="RD477_000284"
     CDS             complement(74137..75024)
                     /gene="xerD"
                     /locus_tag="RD477_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDT7632679.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(75120..75593)
                     /locus_tag="RD477_000285"
     CDS             complement(75120..75593)
                     /locus_tag="RD477_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7632680.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(75698..76561)
                     /locus_tag="RD477_000286"
     CDS             complement(75698..76561)
                     /locus_tag="RD477_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDT7632681.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(76757..78856)
                     /locus_tag="RD477_000287"
     CDS             complement(76757..78856)
                     /locus_tag="RD477_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDT7632682.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(79076..80185)
                     /gene="rpoD"
                     /locus_tag="RD477_000288"
     CDS             complement(79076..80185)
                     /gene="rpoD"
                     /locus_tag="RD477_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDT7632683.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(80209..82050)
                     /locus_tag="RD477_000289"
     CDS             complement(80209..82050)
                     /locus_tag="RD477_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDT7632684.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(82232..84547)
                     /locus_tag="RD477_000290"
     CDS             complement(82232..84547)
                     /locus_tag="RD477_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7632685.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(84732..85592)
                     /locus_tag="RD477_000291"
     CDS             complement(84732..85592)
                     /locus_tag="RD477_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDT7632686.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(85651..86283)
                     /locus_tag="RD477_000292"
     CDS             complement(85651..86283)
                     /locus_tag="RD477_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDT7632687.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
BASE COUNT        23754 a        18222 c        14626 g        29702 t
ORIGIN      
        1 gtataacata gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta
       61 attgttaaat accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca
      121 ttatatccag aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg
      181 tcgaataaac tgccaaagac atacaatcat attgctttta aaattgacga acaagatata
      241 gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt
      301 gtgaaaggcg aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta
      361 cataccggta ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa
      421 tgactagtct ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg
      481 aattgatctg ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt
      541 catcacaaat acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc
      601 tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat
      661 ttttgtctca gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg
      721 ggtatagact tttctcatat tctaccacaa ttttgggttt ccctaacaca tgataggatc
      781 tcacaacaaa ttgctttcta ttattataat aacctgtaac gactaacttc atcccttcgt
      841 ctacttcagc taggaagttc aaactatggg aagcaatcaa acaattgacc ccatttaaac
      901 tgaaacggat caacggtctg gcactaactt gcaaagtacg gatcttatct actgttccat
      961 attttttctc cataaccttc acctcaatac cattatacga acatacattc cctttgtaaa
     1021 gaagaaactc tgttttatct ttttgacttt ttgtctatta cctttcttaa aatcatattg
     1081 gcacttcctg cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt atagattact
     1141 gttgcttctt tactaattta ggtttacttg ttaagaaaga ttatttacgc aaatgttctt
     1201 caacaatttg ctgagattta tcagctgtat cttccacttc atcttttgct ttttctaact
     1261 tttgaatata ttgtttcaag cttgagtttt cagcagttgc ttcgtctaat ttcttttgtg
     1321 tatctgttaa ctgagtttct acatttgttt tttcttgtgt cagtgttgcg atttgttgat
     1381 tcaaaccatt tacagtattt tgtaattctg aaagttttgc ttgtgcttct tgtagtcttg
     1441 ctgtccaatc tgcattcaga tgattgattt catcattttt ttggttgatc tcatctagtt
     1501 tgacattgat ttcattttgt ttgctttgta gttgtgcatc cttgctgact aattgacttt
     1561 ctaagttttg tttttctgct gccgattgat ttttttggtt ttctaattca tttacttttg
     1621 tttggtattc ttggacatct gcctttgaat cttgcaatgt ctgttctttt tgtgctagtc
     1681 gagcggtaag atctgtaatc gcttgctctt tatctgtcaa tgtatgatca agctgtgtca
     1741 gattctcatt gattgtatca atatctgtct ctccacccca caaaataatt tcatttgcgt
     1801 accgcgtgcc tgtgtagcca attagacata gaaatgcaac gacaatccca ccagtaataa
     1861 atttcttttt catttgcata gctccttaat ccaatacttt tacatgctaa aagtaagtga
     1921 tctcaaacac aatgtcaagc gtttttaaat tacatatgta attttaatat aaatactatc
     1981 aaatactcat ttaatcctcc ttttttatct atttaaaaaa tcttttttat agagaaaaat
     2041 gcaggttaat actttcttaa ataaatattt atggtgaaca aaaacaaaaa agcaagtttt
     2101 attaaacgta acccttttca tttcatagat tactgtattt attacaaaaa acgcggattt
     2161 gtcataaatt gtcatgcttc ttttacctat gaaatatttt cacgtgttaa agtattcctc
     2221 gttgaacaca acaaataaaa ctttttaacg aataaaggag atttttcatg acatcactta
     2281 aaactttact ttttggaaca actttggctg ccggcgctgc attcttcatg ggaacgactg
     2341 ctcatgcgga cgaagcttat accgttcaat caggtgatac tctatcaacg atttctcaaa
     2401 aatacgttgg tgataactcc ttgatcaatg ccattgcaga atcaaattca atttctgata
     2461 ttaacctgat ttactcagga caacaattaa caatcccaac agaaggctct gcacaagcag
     2521 ctgctgaacc tcaagcagcc gttcaagaag caccagtcca agctgaacaa cctgttgtac
     2581 aagaaacggt tcaaacggaa aaacaagcag cgcctgttgc tgaaacacaa cctgctcctg
     2641 ctgtaacaga aactgctgct actccagcaa gcacaagctc tgcaaaagaa tggatcgcac
     2701 aaaaagaatc aagcggttct tacacagcaa cgaacggacg ttacatcgga cgttaccaat
     2761 tagattcttc ttacttaaat ggtgactact ctgctgcaaa ccaagaaaga gtagcagaac
     2821 aatatgttac ttcacgttat ggttcttggg aagcggcgaa agcattctgg gaagcaaacg
     2881 gttggtacta agattcattt attaatcaaa cagtttactt ttatacaaag aaagccaaaa
     2941 agaggtgcga cactagttgt gtcacatctc ttttttattt acctaatcta atgaaacgtt
     3001 ccaagttttg ataagtattt tttttaactt cttcaactga tagctctttg atctcagcga
     3061 ttgtctttaa ctgttcttca agcagtgaga cataatcttt ttctgaaaaa atccccccta
     3121 aagcccactc tgctgcatgt agtccatccg tttcaataag taagtggtcg attgggactg
     3181 ctttagctag ttgccagact gctggattct ttttcaaatc aagaccaatg ctgaaataac
     3241 agcctaaatc aatatagtct tgttgatagt ccaaagaaga ataccaatgg atcaaatagt
     3301 gattagggta agcttggata taatttaaga tctctttttc gcatcctttt gtgtgcagta
     3361 caacaggttt ctcatttatg gctgcaaaag caagctgtcg ctcaaatact tttttttgtg
     3421 tagtcatcgg tacattcgtc caaatattat ctaaccctat ttcaccgatg atccttgctt
     3481 ttttcaaaaa aggctcaact tgttcaaacg tatagctatc tgctttccaa ggatggatac
     3541 cgtaacttaa ggcttgagtc ttacttgctg caagctgtct gttttccttc cattcttctg
     3601 gcgaatcaca attgatgatc gtcaaaatcg tgtgttcctg ctgaaggagt gctaaactct
     3661 gactcccatc taaatgacaa tgagcatcac ttaacatcta attcacctgc caataactga
     3721 taaatcgttt gcttcaactc tgattgctgc agtagccatt cacgtgaccg tcgtttttca
     3781 ggcacttgaa tctcagcaat cacatgtgcc ggacgggcgg aaagcacaag aatcctgtct
     3841 gataaataaa ttgcttcttc gatatcatgc gtcacaagta aagtagtccg ctttgctttt
     3901 ttaggcagct tcaacatcca gtcttgcatc tgccctctgg tcatagcatc taacgctcca
     3961 aatggttcat caagaagcaa gtaagaagaa ggattcatga ttgttcgtaa caaggccgtt
     4021 cgctgcttca tcccgccaga taattgttcc ggcaaaaaat gttcatagcc gtttaaccca
     4081 aagatatcca gataatccct tacttgttct tcattgattg ttagatgatt gattttttga
     4141 tataacgtaa cgttttgata gacgctcagc caaggaagta acatatcttc ttgcggcata
     4201 taactaaatg ctgtatggag accagttact ttttgatcgt ctactttgat cgttccagaa
     4261 gtcataggct ggactcctgt taaagttttc agcaatgttg acttaccggc tccgcttggc
     4321 gcgacaatcg atgtaaaggt atctttaggg atagtgaaat ggatgtcttc taatattttc
     4381 aggttttcaa tgactacatt caagtggctg atttggattg acatatctat cactcctatt
     4441 ttataaactg attagtgaat agttcttgcg aaggtacatc ttgcttgatc aatccgtttt
     4501 cctgcataaa agctgtataa ttgcgccaaa ctttttcttc catcaatccc caattctcag
     4561 gatcatccgt ataatttttt gacaagaatg cttgtgattc ttctaaaaac gtccgatcat
     4621 tttcttttgc atatttttgc aacagtttag cactgtcatt tggatcctta atagcttcct
     4681 gatagccttt tttcgtggca tccataaaag actgtaccaa ttctggatca cttttgatca
     4741 gttgatcatt cgtaataatt accggagtat agtaatctaa ccgttcatct aattctttca
     4801 atgggatata gtttagttca atacccgctt cttttgcttt gatcatatcc caaccttcaa
     4861 agtaccattg gatatctgag gattttccta agctttctgg cccttctcca ttgcttccta
     4921 ccattgtcag ttttgaaaag tccccgccgt ctttttccat cacagctttt aagacagctt
     4981 cttcacttgg actttgccaa cctgcatatg tttttccttc aaaatctttt ggtgaatgga
     5041 tgttactgtc tgatagggtg gcgaatccag aagtattatg tttgatcact gtagcaattg
     5101 ctttgactgg tatattctgt ccatctgcgt gagcataagt gacatcttct tgataactta
     5161 caccaaattg tgctttgtcc gcacctacta gaccaatact tgtcgaatta tctcctggtt
     5221 cgatgatttg gacatttaaa ccagcctctt tataatagcc tttttctttt gctaggtaga
     5281 tgccagtatg attagtattc ggtacataat ccaaaacaag tgtcactttt ttcaaatcct
     5341 ttatttcttg tttgctactt gtttcttttg cagtgccaca agcggataaa agcaacagta
     5401 gcggtaataa aagccataat tttttcattt gatccccttc tcttaaaaaa tataaagcat
     5461 acgataatac ccttttttca ataatgaaca agcgccattc agcaataaac tcaatagaat
     5521 gacacaaatg atcgcagcaa aaactttatc gatctggtag ctgtttttta cgcgtatcat
     5581 gtaataccct aagcctgctt gagcgcttag ccattctcca acgactgctc cgctgacaca
     5641 ataagtagca gccactttta aaccggaaaa gaagccggat aaacccgctg gtattttata
     5701 tattcgataa atcttccctg tatctgcccc catcgttttc aaaaagatga tttgctcttt
     5761 tgatactttc ctcaatgtat ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac
     5821 aacaaggatc acttttggaa gcataccgaa accaaaccat agggtaagta atggccctaa
     5881 caccatgatt ggcaacattt gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa
     5941 cttccaataa tccattgcca aggcagtaat aaaagaaaga acgctagcta ttaccaaacc
     6001 aagcactgct tcatttaatg tcgtgacaga atgggaaagt aatacttgtg catctgccgc
     6061 taatgcagaa aatacagcag caggtccggg taaaataaag gcaggtattt tccaaactat
     6121 gacaacaatc tgccaaataa ttaaaatgaa tgccatactg actgctggat aaagattttt
     6181 cttcattttg ttacctcctt tcttctaata tgaataacaa aaaacgcttg aaatgggcac
     6241 gacatagtaa cccgcttcaa gcgtctgcta tgtttctccc ttcaccagta ttaactgaca
     6301 ggttcaatgg gtctaagttc acaacttact ctcatgtcgt ctacgacatc cccagaaaca
     6361 ggtattcatt ttttaaagtg tagcacaaga aggaaaaagc aacaagcgct ttagttatca
     6421 tgctgattat ttcaataaat aggagagatc acttgccaat gtcggatacc ccatgatata
     6481 gctatccaac tcttttttag tcaatttttg attgatgacg agtgtcaaat catcgatcaa
     6541 ttcgtctgct tgctcgctaa tcactgcagc accaatcaaa taattttgct ggtcaaaggt
     6601 caattcagcc ttagcaactg gttcattgac acgatgatag gtgaaccaac tggtaagatc
     6661 catctctacc acttgatctg acgaagaggc gtgacttttt gtaactccta cttcagcaag
     6721 tttaggactg gcgtagacga tcgtaggtat aatcggatat ttgataggtt cgcttgttgc
     6781 atccgttatc cgcttagcta cataatttcc ttcaaaagtg gcgactggtg tcaattttgg
     6841 ggttttcctt gaaacaatat ctccacacgc aaaaattttt ggattgctcg tttgcagata
     6901 atcattgact gcaattccgt gtttatcaaa gacgatattc gcttgctcaa gtgctaaaga
     6961 ttctgtattc ggctgtctcc cagtcgcaca aaaaatcata tctgctacta attccgtctc
     7021 ttttccgact aaacgatagc gagtaccttc tgaaatgatc ttttgggttt caacaccaaa
     7081 agcaaactga atgccagatg cttccatttg atggacggct tcctctacca aagacgcctc
     7141 aaattctttt aatggccgtt gattatgatg gacaatggtt actttgctcc ctgcagcatt
     7201 ggcaatcgta gctagctcaa aagcaatata accaccaccg ataaaaatga tttctttagg
     7261 cagtacaggc aatgataaaa agtctgcact agttttcaag tattcctttc cctccactgg
     7321 taagatcgta ggtctttgcc cggttgccaa aactaagtaa tccgcatgga agacttcttc
     7381 gttgacttca atagatgaat catccaaaaa acgagcggtt cctgaaagat gatcgatttc
     7441 agcttctgct aattgttttt ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa
     7501 tttttgaagt tcttcccagt tggcagttgg gatctcgtta aaaccttttc cactcaattg
     7561 ttttacccga ttacgtgcct ctactgcact taacaagact tttttaggat cacatccacg
     7621 gttaggacaa gtcccgcccc atagatcttc ttcgaccact agtactgttt tacctgcttc
     7681 tttcaatcca taagcagcac ttaagccgct cacacctgaa ccgatgatga ttgcatcata
     7741 tgttttcatt tccttcctcc tctcgactta aaaaagcaaa gatttcttta cttgtcagcg
     7801 acccaatact tctttgcttt tctactatca caatgcttgg atacaccgaa agaagcgaat
     7861 atacttcttt cataggcgta tcaaagcaaa cttgttgacg agcattttct ggtatttcat
     7921 ttaagaaata tccattgacg actaaatctt ctactcgcca ctctttcgtc tgtttcggtt
     7981 catcaaagaa atgccgaacg tagtcgtttt taggattcat caataactgc tctggtgtgt
     8041 cacattggat aatttctcca tccttcataa atgcaatacg atcacctagc ttgattgctt
     8101 cttccatatt gtgcgtaacg aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa
     8161 ccaattcttg taatgagttt cttgaaatcg gatctaatgc gctaaatggc tcgtccatca
     8221 aaatcacatc tgggcttgca gcaatagctc ggatgatccc gatccgttgc tgttctcctc
     8281 cagacaactc tctaggcata cgatctcgat aaatatcagg agataagcca actttttgaa
     8341 gaagttcatc tacacggtct gctcttttct gcttttccca acctaacatt tcagggatga
     8401 cttcaatatt ttgcttgact gtcatggtcg ggaacaaagc gatttgctgc agcacatatc
     8461 ccatattcca ccgcattttc tgcagattat attcatttaa cgattttcct ttgaagtaga
     8521 tgtctcctgc tgatggttta gataatccat tgatcatttt caaagtagtt gtttttccac
     8581 taccggaagg accaaccaaa acaaagattt ctcctttttt gattgccaga tttaggtcat
     8641 cgatgacata ctttccatca tacttttttt gcacatgttt gaactcgatt aatttagtca
     8701 ctgatcttcc tcctttacta atccttcatt tttcaggaag attcgtgcta cttctgtcgg
     8761 ctttttcttt tcgacattga cttggtaatt catatcaatc atttgatcct ctgttatttt
     8821 tccgcttaat ttattcagtg ctttaacgat ttcaggatgt tctttagcaa aatctttttt
     8881 catcagcggt gcgccttgat aagctgggaa taaccctcga tcatcttcta gagtcactag
     8941 atcgtactcc ttgagctggc tatctgtgga ataggcatcc acaatattga catctccatt
     9001 attgattgct tggtaacgca aacttggctc catgctttgt acacttgaaa aattcaagcc
     9061 atatgcttct tggattcctt tatatccatc tgaacgatca ataaattcca gtgtgaatcc
     9121 cgctttgatc tgatcagaga tagctgccaa atcggaaatc tttttaagtt gatgttcgtt
     9181 cgcaaaagat ctcttgactg ccaaggcata cgtattttgg taagccattg gttttaataa
     9241 tgtcatatcg aattgcttat tcaataattc ttctgcttcg tcatatgttt tttcttttgt
     9301 caaatcagca cttttcattg cttcaggtac cttcaccaag ctttctagga cagtacctgt
     9361 gaactcagga tagatatcga tttgaccttg atccagtgca ttgaacaaaa aactcgtctt
     9421 gccaaaattc ggttttaatt caacttctgt ttttgtgttg tcttcaatca attctttata
     9481 catttggatc aggatttcag gttccgctcc taattttcca gcaatcgtga tggtttcatt
     9541 tttaaacaag gcagactgat ataaagaaac gccgccaaat aaacacacca ctgctgctaa
     9601 agtaatgatg gaatatcgag gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat
     9661 aattgcaagc aaggcagaac tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc
     9721 aatacctaac aaaataaatg tccctaatcc acctgctccg atcaatgctg ccaaagtagc
     9781 cgtaccgatg attaaaacta aggcagttcg gatacctgag ataatgacag gtaatgcgat
     9841 gggaagctct actttcagca agcgtctcat tctggacata ccaaatgcta ccgctgcttc
     9901 ttcaatagac gggtcaattt cagctagacc aatatacgtg ttttggaaaa tcggaagcaa
     9961 agcgtaaata acaagtgcaa tcaatgctgg aacagttccg atccctacaa atgggataag
    10021 taaacctaat aaggcaagtg aaggaatcgt ttggagcaca cccgtgatct gcaataagac
    10081 ttctgccatt tttttatgat tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc
    10141 aatcagcaaa gaaatgagag aaatggacag atgttgaaat gcagcttcca gcaactcgcc
    10201 ttttctttca cttaatgttt gtataaaatt attcatgcga ccttcctttc tgttattctt
    10261 taagtataaa catttctttc ctttttccca actaacttgt cttgccttaa ccttacagca
    10321 cgaacaaaaa aaagaaaaaa atacctctaa atatgctgac aaaaaaagaa tccaagcaac
    10381 aaagtcggat caactttttg gttctcgttt tttcactacg aataccataa aaatctatac
    10441 gaaattgtca cttggaatca gctatatctt ttttacacac acaactttta ttttaataaa
    10501 aagaaacagc caataaagta gaaatctcgt gggggagaac acctttattg gcctgttcaa
    10561 cgaatattat actgtatcat gacacttttt tgccaaaaat gtaaaaaatg gttgttttga
    10621 catcataaac gtcttgtttt cttattttta cttatatcac cactttattt tctatctctt
    10681 tcactccata aagaggaatg agttcttctt ttgactgata ataaacagct ttctcacggt
    10741 tcttcatcaa aaaaagaaag atgtctttca tagcaagtgg tgctttgccg tgttcaaata
    10801 aaaagataaa ttgatttgct ttatttatct ggaccattgt cacaggcacc agtttttgtt
    10861 cttgagccaa aaaaactcct tgatactctg tttttttggg tgattttgta agaattgtca
    10921 ataagtctgc tgctcgcatt tttccactcc ttatttgata aataaaaaga aacagccaaa
    10981 aataattttt tttcattttc ctcttgaagt aagagtataa gtcagtataa taaagaagtt
    11041 gctgaaaatt ttcaaaagaa aagaggctag aaatgaaaaa attaaattta tttacagaat
    11101 tgtgcggaat cattttaggc gcaggaatct acggattagc tgtgacagga atcaatattc
    11161 catctaagct ggcagatggt ggggttactg gtattgcttt gcttttgcat aatctttttg
    11221 gattcgctcc ttctgtcact tcactgcttt ttaatttacc tttattgctt atctcattgt
    11281 ttctttttgg aaaacacgct tttctccgga cgatcgtcgg aacatttgcg ctagttttct
    11341 ttttacattt atgggagagc cttcatatac attttgctgt agatagtcta ctgatcaata
    11401 gtttgatgac aggtattctt tctggcattg gttgcggtct cgtctttcga ttcggcggaa
    11461 gcacaggcgg aacagatatc atttatcaag ccattgaaaa atattttcat attaagattg
    11521 gcaaaagtct tttcttgatt acttttggta ttttagttgt ctccttgctt tatttggatc
    11581 tcacacattt tctgtataca ttacttagct gttctattct atcttataca ttgaataaag
    11641 tgaagtatct ggaaattgga tctgttttgc cccaattgga agcaaaacag actacagaaa
    11701 catttgaacc acttgatcct cttgaacagt tagagcactg attcttattt ttcactactt
    11761 tatttaattt acagaaatat cattatgtaa actaaaatga tattattcag taaaatcttc
    11821 tttataaaaa aataaactat ttttgcaaac gatctgcgaa aatagtttgt ttttttattt
    11881 tgccattatt ttcttactta ttgggagaaa aaattcgctt aaactcaaaa aaatttgagg
    11941 atacgatata aactttcgga tagagtataa aataaggaaa cgccgcagcg tttcctttcc
    12001 aactgtagcc gaatataaat ttgtatatca cttatttaaa atagctattt tctcccaaag
    12061 agtttccttg atcatcgatc aatccttgta atgagcgttc taagatacca tggctttttt
    12121 tcgatttttt ttcttctgac tgctttgttt cctctaacgt ttttgttcga ttgaatgttc
    12181 gtacagacgg ctcaccattt cttttttctt gatacggcgt cggttctgta tcgaacaaca
    12241 gataagagtc atccgttttt ttcatcgatt cgattaaatg atcctcagaa attttgcttt
    12301 ctctagggtc tgggataatc gatgcgggta catatttagg aacaaaataa gaacgctccc
    12361 gcaccttcgt gtcaacagat gcgtttcgtg tagtatgctt ggactctgtc cttgtttcac
    12421 tttgtctgac agaacgagag gaatcaccaa aaagtttttt tcgtcctgtc gatgtgattt
    12481 ctgattggta atggccggta taatccggta attttccttt atgcttttcc aaattttcct
    12541 tacgcggagc tgacttcaat ccatttgttg cacgacgctt gcgacggatt ttctcggtgt
    12601 tcgtgttcgt gaaatgaaat tctttcatct gttcaaacgg ggactgattc tgttcagtca
    12661 aaagatcttc ttgtccgtca aataatactc gcttcttgtc gttggttaat tttacgccta
    12721 actcatcatc gtacgcagga aaacggaaat gttttcgtct gatttctttc attcaaacca
    12781 tccttctcaa aaatggaact tttctcaata atagcacaga ttcattgttt tcagtagcaa
    12841 tgcgctgatt attttaagaa aatagcgatg aatttcataa atatatatat agtaataaga
    12901 cttaattcgt tgtctctctg ataactagtt caggaaaaaa acgacgataa ccttgactcg
    12961 cccctttttc ttctattttt tttaacaaca tttctccaca tacttcaccc atacgtataa
    13021 tatcttgctt gatcgttgtc aatttaggtg aactgatttg gtctaaaaat accccatcaa
    13081 atccaatcac accaaattct tcgggcaagt gtcctccagc atttataatc cctcttactg
    13141 ctcctagtgc tagccgatct gaactacata caaaacaagt tttatctgta aaatctgccc
    13201 agttttctgt catatatctt tctacttctg tcgaatgatt tggaaaccgc aagattttac
    13261 taggaagatc aacttccttc atcacagata aataaccagt ttcccttgaa atttcaaaag
    13321 gctcgtccac ttcgataccg atataaataa tcgtttgata atctctttct aaggcatatt
    13381 gcgttgcttt tgctgtacca tattcattat cgctgtctac ataatcatat ccttcagaat
    13441 tttccccaaa caagacaact ggtttttcca aacggtcgat ccaatcgaag tctttttttc
    13501 tgacacctgt gatgatgtat ccatcacaag aaccgacgtc gaaggcatca tttgtaacta
    13561 attggagaga ataatgcgct ttaccgatcc gtttagcgat tcccatcagc aaattcatgt
    13621 aatagggctc agttgtgtcg atttcttcca gtataaataa tttgatgatc tgcgaccgat
    13681 tcgtgacaag tgcttttgca gcaatatttg gacggtaatc cagttcagcc attgctgaga
    13741 aaactaactc tttcaattcg tcagtcacct gttccggatg attgatgact cgagagaccg
    13801 tcatttttga aacattggca cgttttgcaa catctgctaa agtgggcatg ctctcttcct
    13861 ttctaacatg ttgtcattct tactatcaat tataccaaat tttcctaaca gagtttatta
    13921 tagcccaaaa taaaaaacgg aaaatcctct atcgtcatga gtgattttcc gtttcaatta
    13981 tttatacttt tttatcaaat tttttgctaa aaaagtggaa gcttcttcca ttagtggtat
    14041 ttgtaccata tgtcgctgtg tttttgagga acgaaaaaca atttcttttc cgtaagactt
    14101 atgccgattt ttttctacaa atcgttccgt ttgtatataa gggatttttt cgtcagcttg
    14161 tccatgccaa aagaacagag gacgtccagc taacttttcc ggctgcaatg acaaatcata
    14221 gctcttaacc catgaaatca aatcaaaata gtcttttggt acgacataat tatattccga
    14281 tgcgcgcctt acgatttctt ttccatattc caaaggtgca ggggaaccca tcaaacacac
    14341 gcccgctact atatgaggat gtttagccaa caaggcgcat gttgtcattc cccccatcga
    14401 aaccccgcca actgcaagct gatcatgtag aagacctctt ttttctaaat gcttgataag
    14461 tgtgtcaaat tcaaacagat ttccgtagat actatcccaa aatgtatagg atggaataga
    14521 agaaaccggc tgtttgcgct ccccatgatt catcgcatct ggcaggatca cacgaatgcc
    14581 ctgctgcgca atttttcttc cttgcgtcaa aactaactcc ttacttgtct gccatccgtg
    14641 ataatagatg acaacgggaa tacgttcctc tcttaattct ttcagaacaa cttctaacac
    14701 tggaatatga tggacagaac gtttcaatat aattggattc aattgttact cacctatttt
    14761 cattaaattt cctgatcgcc atagcaacca tgctccaaca gttgtctgga acaggttcag
    14821 tgtaccaaat aatataaatg caggactgaa tggctcggga aataagcaca gagaacaaag
    14881 tcccaccact cctaatgcta gcgaaaggag tggataaata gaaaaattca aaaaactggc
    14941 aacgctaccg aaaatattac caataaacag tatcaaaaag aaaaaaacgc ttaaaattaa
    15001 accatctgtc agcgacatct gaaaaacaat gttctctacc tcaattggct gttgaatcga
    15061 atagacacct aagtatatac tgagaaggag acttagcacg tggctggcac aaaacacacg
    15121 attgaccgtt ttcattttaa tcacctaacc tatctttata attctattat aatcatttta
    15181 tcattttttt gtatgtataa agtttcaatt tttcttcact tacgattttt tttgatattg
    15241 ttgttatata aatgaaagga gttttacaaa tgattgatcc aaaaatgttg gggatgatct
    15301 tcctcattaa ttttgcttat atcactctga acacgttacg tttcatgttg acaatgaaag
    15361 gctatcgact gattgctcca cttgtcagta tggtcgagat cacgatttat atcgtcggct
    15421 tatcccttgt tttagatcgt ttagataatc cactgaattt atttgtctat gcgttaggtt
    15481 acgcagtagg tatcagtgtc ggaattttga ttgaagacaa attggcatta ggaaacatca
    15541 tggtcacaac gattttgcca gcaaatagcg cagaagataa aagtttgccg ggtatactga
    15601 gagaacacgg ttatggtgtg acacagactt ctgcaatggg gctggaaggt gagcggctag
    15661 ttctcgaaat actttctcct agaaaaagcg aacgtgaact gtatcgcctg atcaaagaaa
    15721 tcgaagagcg tgcttttatc atttcttatg agccaaaata tatttcaggt ggtttctgga
    15781 ctaaaaaagt gagaaaacga caaggaaaat aactcacaaa cttaaaaact gtctaatatc
    15841 taaattaaat tttaaatgtt agacagtttt tatttattat tttgattaaa aaacatcgac
    15901 atatactcat caatttgtta ttatacttta tatacttatc aatttaattt ggagatatta
    15961 tggaaattca acgtagaacc actaattttt tacatttagg ctcttttttt gcaactttga
    16021 cgatcttatt ttttctatgt tattcatacc ttaagacctc acatgcgcct tctagtgcat
    16081 taggctggtt gcttttggat cattctgata tcacagaagc cgaagaaaat ccgttttttt
    16141 tgattttgtc ttctctttgc ggcttactgt tttctgttta tttcggtgcc tatttttttc
    16201 atcgacatcc tggtacgtta aacgaagctt caagcgtatt gattgcattt aaaacgtttt
    16261 cttttttagc aattcttttt tattcgttta atcacaatac gcttttcttc tttatcatga
    16321 atggtttagt cgtgatttta tccattttta cttattttat tttttcactc atttttagcc
    16381 aattgaaatg tccgctcttc tttcgattag ttcctgtcac ctgttttatt tacagcttgt
    16441 tttccttttt tgtccttgct ttaatgccac tgaccaaccc ttcgacgatc caagtcgcag
    16501 gaaatctgtt ggatatgctc ttcatcgtca gtctaggtgt atttatgtgg caaagaaaaa
    16561 acaaacaaaa taaaaagcat tttgaagcct gactattacg acttcataaa tgctttttta
    16621 gttttaaaca cttcaatcta aaagaataac ccaagattgg ataaattatt aatgactcgc
    16681 cattcgtttc ttagtgattc tggattatca aatggacttc ctaatctaat cagctagttc
    16741 ttcccataaa atgccatact tgactgaatc ataatattga ttatttaaaa atctaacttt
    16801 tcttatcact acttcctttg tcattccaca tttctcacca tttttttgca ttcctttgtt
    16861 tccagaccat gtagtgaatc taataggtgg tagataggca aaatactcaa ataattcttt
    16921 taaccataaa ggaagagaaa ggctcctcct gcaagagtca gtataatttt tgcttactgc
    16981 tctcttaggt tttactcact aaaactgttc catattcttt gatataagaa aatgcatgcg
    17041 gtataaattt cttgctcagc tcagaaaaga tttgtcgttt aagaaaggcc atttcctcca
    17101 ttgtcaatga aggatccaat gttggtaatt caatctcaaa attgtttttt tcgacaatag
    17161 gtaattctaa tgtgaatgat tcttcttcct ttatagtatt cgtcacaagt agagactgat
    17221 ggaaaatact aggccggcga tttttatatt tattcttacg tatcctgtca acgataactt
    17281 ctatttgatc aaaaaagtac gattcattta cgtctccaat aagttcggct aattctttga
    17341 atgccttcgt tacaggaagc tgattcttct gtacaaaaaa gtaattagcc aaagtaagcg
    17401 ggaagtgttc tatagaggaa agtgtgataa aacggtactt ctcatttcct gcagttgtcc
    17461 tcagcaattc tgtctctcgg ctgtttctta ctccataaaa atacgcttct ttccgttcat
    17521 cttcgaaaca atacgcccaa ataacttctc ccatcgctaa tttttgttca tcaacagaga
    17581 gtctcttcaa ttgttcagct atggtttcag cagccgaagt ggcttgttgc aagtaatctt
    17641 taggatcaaa tattggaaag ttcaaatgat atcttcgttc ttccctcttg attaatcctg
    17701 catcgatcaa taaatcaaga aaatgggaaa atttattttg cgaaaattct tttttcaatt
    17761 ctcttagaat cgtttcttgt tctcgatgtt catatagata atcggtcaat gaagaaaaaa
    17821 gcggatcatc ggtcagtttt tgataaaagt tttccttatt ttttgaataa tagttcatat
    17881 atgttctctt ttctatccgt tttattttac ttaaaaggct tttacgtaaa aaggaggaag
    17941 aacaagagtc ataattgact gttattctcc tccatttctt cggcctttag tatgttctat
    18001 gtttcttttt atatcaatcg cttatccatt cattttgaat gcttcatact accatttgat
    18061 tagctaaata cagccgtcaa ttgaggtacg acttgttttt tacgtgaaac gacaccttta
    18121 agcaaagcac ggttgttggt caaagttgtt tggaaagctt cttctacttt ttcttttcct
    18181 ttaccagcaa caagtaattc tgaatcactg tctaagatgt ttgtgacaac taatacgaac
    18241 agatcgtagc catttgcttt catttcatct tccattgctg cttctagctc tgcttgtcga
    18301 tccatcactt cattaaggtc cactgtattg acttgagcga tacgaacgtt ggaactgccc
    18361 attgggaaac tttttgcatc caaatcaaga agaaccgcag ctgatttatc acttagattt
    18421 gttcctgctt tcaacatttc aagtccataa gtgtttagat cgacttcagc gattgctgca
    18481 agttcttttg cagctgcttc atcttcaggc gtacatgttg gtgatttgaa caacaatgta
    18541 tctgaaatga tagctgagag catgattcct gcgatttctt taggaattgt tacttggttt
    18601 tctttatata gttttaaaat aatcgtactt gtacatccaa ctggttctgc acgatagtac
    18661 aatggattag ctgtttcaaa gtttgcaata cgatggtggt ctactacagc taaaatattt
    18721 acttcgctga tatctgaaat actttgttgg aattcattat gatccacaag catgacatct
    18781 gcattttctg ctttagctga ctctactact cgtggtgcag atagctgaaa gtggtctaat
    18841 gcgtattgtg tttcttcatt aggtgtacct agagcaactg cttctgtttc tttacctaat
    18901 tcattttgca agtgtgaaaa cgcaatggct gccccgattg catctgtatc tggattttgg
    18961 tgaccaaaaa ctaaaatttt tgacatgtca ttcgctcctt tgtgatttct cctatttaat
    19021 aatagcaaaa aaacggaaag aagcaatact tatcttcttt ccgatgcgac ctatcgcgtt
    19081 aaatattttg tatactcttc tgtatgaagc aatcgtttag cattttcgac acgttctttt
    19141 gtaggtggtt cgatcccttc taatggatat ttcaaaccaa gtgtttccca cttgtatttt
    19201 cccatcgtat gataaggaag aatctctact ctgtctacat ttgacaaggt ttgaataaag
    19261 ttgtctagtc ggatcaaata ttcatcataa tcacttcgct caggaactag tacatggcga
    19321 atccaaaccg gtttgttgat ttttgacaaa tattgagcca tttctaaaat attttcattg
    19381 tcccaatgcg ttagtttttt atgctcttca ttatcgatgt gtttgatgtc aaataacagt
    19441 aaatctgtat acttcatcaa ttcttgaaaa cgtgaaaaga aaggttcttc atacgtaaaa
    19501 ggttttccgc atgtatccaa tgtagtatgc atcttccgct cttttgcttt tttaaatagt
    19561 tcgatcaaaa aatcgatctg caatagaggt tctccaccac taacagtaat accaccttta
    19621 tctccccagt aaggtctgaa gcgttctgct ttatctaaaa gttcatctgc tgtgtattct
    19681 ttccctccgc caatattcca agtatctgga ttgtgacaaa attggcaacg catgcggcat
    19741 ccttgcataa acacgacaaa gcgcagtcca ggtccgtcta ctgaaccgaa agtttcgata
    19801 gaatgaacat agccgattgt tttttcttcc atttaagttc acatcttttc tttgatttca
    19861 ttttaagtac aagttagctg tcgaagcgaa gctttctgaa caaaattttt cgcaattgcc
    19921 gcttcgttat gtgtaaaatt tacagggacg gaacatacaa gtcctgcccc tgtaaatcgt
    19981 ttctttcaaa taagcatact gattattcga ttttgatgcg atggctattc aacgtccttt
    20041 cgacgctaag tcgaaaaact tttttcacga actttttaca ttctgtcgtg tgaagttctc
    20101 atcaagacat ctaattgttg ttctcttgtc aagtcacgga atttaactgc ataaccagat
    20161 acccggatcg tcaagttcgg atatttcatt ggattttcca ttgcatcacg caataaatca
    20221 ttactgaata cattgacatt caaatgataa gctccttttg agaagtatcc atctaagaca
    20281 ttacgcaagt tttcgattct tgtatcttgt tcttttccaa gacctgaagg attgattgtt
    20341 tgggtatttg aaattccatc caatgcacag ccgtaattca ttttagctgt tgagttcagg
    20401 ctagctaata acccactttg ttccgcattg taacttggat ttgctcctgg cgcaagtggt
    20461 tttccagctt cacggccatc aggagtattt cctgtatttt taccgtaaac aacatttgaa
    20521 gtaatcgtca ataatgaagt tgttggtttt gagttgcggt aagttttatg acgtttgatt
    20581 tgtcccatga agtattccaa gatccatgaa actaattcat ctgcacggtc atcatcattt
    20641 ccgtatttag ggaagtcgcc atccacttca aaatcaactg ctattccttt ttcattacgg
    20701 attgctttta cttttgcata tttgatcgct gacaagctgt ctgccgcatg agagatccca
    20761 gcaatacctg tagcaaatgt gcgttgcaag tcagaatcca tcaaagccag ctgagctgct
    20821 tcataagaat atttatcatg catataatga atgatgttca acgtattgac atatagttct
    20881 gctaaccaat ccatcatatt catgaagtta tgtttcactt cttcaaagtc tagtatttct
    20941 gatgtgattg gacgatattc tggtccaact tgggcgccag tcacttcatc gactccgcca
    21001 ttgatggcat ataatagtgt tttagccaag ttagcacgag cgccaaagaa ttgcatgtct
    21061 tttccgacaa ctgttcctga tacacagcaa gcaatcgcac aatcatcaga accccattgt
    21121 tcacgaagct gattatcatt ttcgaattga atcgaagaac tttcgatagc gattctgcta
    21181 gcatatgttc tgaatccttc tggaaggtgt tcagaataca aaacagtcaa gtttggttct
    21241 ggtgaaggtc ccatatttgt taatgtatga agcagacgga aatcattctt tgtaactaat
    21301 gaacgaccat ctaatcccat acctgcaatg gataaagtag cccagatagg gaatcctgag
    21361 aataattggt tatattcagg tgtacgcgcg aatttgacca tacgtaattt cattactaag
    21421 tggtcgatca attcttgtgc ttctttttct gtgattactc cattatccaa atcacgttgg
    21481 atatagatat caaggaatgc tgagatacgt ccgattgaca tcgcagcacc attttgtgat
    21541 ttgattgctg ctaaatatcc gaagtatagc cattggacag cttcttttgc atttgcagct
    21601 ggtttagaaa tatcatagcc atatttcgca gccatttctt tcatttgcgc taatgcacga
    21661 tattgttcag tgatttcttc acgtaaacga atcacttctt cactcattgt atgataacca
    21721 gtcatatcat gatctttttt cttttgttcc atcaaatagt cgattccata caatgccaca
    21781 cgacgatagt caccgatgat acgtccgcga ccgtaagcgt ctggaagccc tgtaatgatt
    21841 ttgtttttac gagcagcacg catttcagca gtataagcgt caaatacacc ttgattatgt
    21901 gttttgcgcc agtcagtgaa aatatgtgtc aattcatcgt ttggtgtata gccattgctt
    21961 tttagcgcat tgttagccat attgattccg ccgaacggca tgaatgcttg tttcaaaggt
    22021 ttgtctgtct gtagaccgac gattttttcc aaatctttat ccaaatagcc tgcatcgtgt
    22081 gaagtaatag ttgatacgat ttctgtgtcc atatcataga caccgttacg ctcgaattgg
    22141 atatcattta acttttgcaa ttcatcccat aatttttctg ttgcttctgt tgggccttcc
    22201 aaaaagctgt catcaccgcg atattcagtg tagttatttt gaataaagtc tctagtgtct
    22261 acgccttctt tccatttggt acctttaaat ccattccatt gctccattgt atggacctcc
    22321 taaatttctg ttttatgtaa cagtttttac gtgattagta taacacagca ccctatcttt
    22381 gcaagggttt tcttgttttt tatttaacaa tttttatgtt ttatttacat atatttaaat
    22441 caacgttgac ataccaacat ttaagcattg tgaacacttt catctttttt tgcaaacgta
    22501 taaggattta ttaaaaatga atgaaaaaga gacgccacct gtattaatac agatggcgtc
    22561 tcttcattaa aatactacaa cagttacgac tcgatgaaaa tctcaggagc atcgataact
    22621 tcgatgactt ctccaccttt tttctcatcc atcgcaaatg atccattcga tgttcgatca
    22681 cctataggga aatcagttgg gtcaatcgtt aactgctgcc ctttttgatt catcaaggtt
    22741 atttcttttg atcttccttc cgacatatag ataatacggt gcggattttt tttcagctca
    22801 cgcatgaccg tcactccccg cttcgcacgt gaagtttggc tgatttcttg agcaagcatg
    22861 cgtttaacag ctcctcgctg agtaacgatt acgatcggcg tatcaccttc tgctaacaca
    22921 agcagcccgt tgataatcct atctccatct ttcaaattca tagacttcac tccagctgcc
    22981 tttgctccaa caacgggaac atctgttaaa ggatagcgca aacccattcc ttgatgcgtt
    23041 actaagaaaa catctaagcc tggctgatct tctttcagat acacagcaat cagttcatct
    23101 tcttccgcct ttagcttcat acagctgagc ggacggctct tataggtgcg ccatggttcg
    23161 aactctgtca ttcgtgtttg cttgatcaat ccattcttgc tgatgaatac aaatgttttc
    23221 tctgcttgaa ttttttgata tgggaaaaca gctaaaatcg attcgtctac agacagattg
    23281 gagattgttt gagaaatgtg ctcaccagca tctttccatt tcaaatcggg tagttcatgg
    23341 acagggcggt agatcacatt tgctttattt gtgatcagaa gaacatggtc caatgtactc
    23401 aattcacctg agtatagcaa gtagtcgcct tctcgcatcc cgatttcttc tggtttggaa
    23461 gcactataag aacgaaggct tgttcgcttc acatagcctt ctcgcgtcac actgacaatt
    23521 acgtcttctt gtgccactaa gacttgcgta tcaattttga tttcttcgat ttcgtcttcg
    23581 attgtcgtta gacgagcgga agaataattt tttttcactt ctcgcaattc ttttttcata
    23641 acagagaaaa gctctttgtc atttgaaagg attttattca actctgtgat ttgcgcaatc
    23701 aagctttcgg attccttttg aagctctgtg atatccgtgt tcgtcaaacg gtataattgc
    23761 aatgtgacaa ttgcttctgc ttgttcttct gtaaattgaa agacgtcgac taaattcttt
    23821 ttggcgtctt ttttgtcttt actttcacga atcgttgcga ttacttcatc cagaatcgat
    23881 aaagctttca tcaatccttc tacgatatgc tgtctttttt gagctttggc taaatcgaat
    23941 tgactacgtt ttgttatgac ttgctttcga tgctcgatat aacttcttaa aatgtctttc
    24001 agtccaactt gatgcggagt catatggtcg atagcgacca tattgaaatt gtaattgatc
    24061 tgtagctcag tgtttttgaa caaataattc aaaattcctt gagcattggc gtcttttttc
    24121 aattcgacga caatttgcag acctgttcta tcactttcat ctcgtacttc agctattcca
    24181 tcgattttct tattcaaacg gatctcatcc atttttttca ccaaggtcgc tttatttact
    24241 tcatatggga tttctgtgat cacgatttgc tgtttattac ccttcattgc ctctatcttt
    24301 gttttcgaac gaagaataac ttttcctcgt cctgtttcat aagctttttt gatttcatct
    24361 tttccttgca agatccctcc tgttggaaag tcaggtccag gaatatattc catcaatttt
    24421 tcaaggctcg cttgtggatg atcgatcata tagattgtgc catcgatcac ttcagctaag
    24481 ttatgagtcg ggatttctgt tgcatatcca gcagatatac ctgtcgatcc attgacgaga
    24541 aggtttgggt atcgagctgg aaggactgtt ggttcttttt ctgtatcatc aaaattccac
    24601 acgagatcga ctgtttcttt ctcgatatct gctagcatct ctccacttaa cttggataat
    24661 ctggcttcag tataacgcat cgcagcaggt ggatcaccgt ccatactccc gttattcccg
    24721 tgcatttcga tcagtacttc ccgcaacttc cagtcttggc tcatgcggac catcgcttca
    24781 taaatactga tgtcaccatg aggatgataa ttccccatga tatttccgac agattttgct
    24841 gatttacgga atcctttgtc atacgtattg ccatctttgt tcatggcaaa taatatccgg
    24901 cgttgcactg gcttcaatcc atcacggata tcaggcagtg cacgttcttg gataatatat
    24961 ttggaataac gtccgaagcg gtcacccatc acttcttcta aagttaattc ttgtacttct
    25021 tgtcgatttt ccatcaggtg acctcctatt ctacttcaaa taactgattg ttttcatttt
    25081 tatctgctcg ttcttcatct aataaatcat tagaaactga aggagagatt tcagtatcct
    25141 cttttttatc taagatactg ccatcttctt cgagcgtaaa ttgcacgtga ttttcgatcc
    25201 attttcttcg aggttctact ttgtcaccca tcagtgtggt tactcgacgt tcagcttgcg
    25261 cagcatcatc gatacggacg cggatcaacg tacgggaagt cggatccatc gttgtttccc
    25321 acaattgttc cgcattcatc tctccaagtc ctttatatcg ctgcagcata tatcctttgc
    25381 cgactttttt aatcattgct gccaattcgt catctgtcca cgcatattca atgacttgct
    25441 tctttccttg tcctttggat actttatata aaggcggtaa cgcgatatat actttcccag
    25501 cttcaatcag cggtttcata taacgataaa agaaagtaag cagtaatact tgaatatggg
    25561 cgccgtctgt atccgcatcg gtcatgatga ttactttgtc gtaattacag tcttcgatag
    25621 aaaactcagg accgactcct gcaccaatcg tatagatcat cgtattgatt tcttcatttt
    25681 tcaagatatc ttgcatttta gctttttcgg tattgatgac ttttcctcgt aaaggtagaa
    25741 ttgcttggaa ctttctgtct cttccttgtt tagcagatcc gccagcagaa tccccttcga
    25801 ctaaatacaa ttcatttttc ttagggtttc gggattgtgc gggtgtcaat tttcctgaaa
    25861 gaagagattc gcctttttta cgcttcttcc catttcggct ttcttctctg gctttacgtg
    25921 ctgcttctct cgcttctctg gctttgattg ctttacggac caacatctgg ctcatttcgc
    25981 tattttcctg caaataaaag cccatttgtt cactgatgac gttatctact acggtacgag
    26041 caactggcgt accaagtttt tcttttgttt gcccttcaaa ttgcagaaga ttttcaggta
    26101 cacggataga aagtacagca gctaaccctt ctctgaaatc actaccttcc agattcttgt
    26161 cccgttcttt cagcaaacca acttttctcg catattcatt ataagatttc gtcatcgccg
    26221 ctttcatacc agcttcatgg gttcccccat cttttgtccg aacgttattg acaaaagata
    26281 aaacattttc tgaataaccg tcattgtatt gataagcgac ctctacttcg ataccttctt
    26341 tttctcctga aaaataaaca actggtgtca atgtatcttt ttcttcattc agataatcaa
    26401 caaattcttt gattccttct tcgtaatgaa aaatttcctt gactggttct tctccacgaa
    26461 gatcagaaag agagatttta acgcctttta gaagaaaggc agattctctt aaccgttcgg
    26521 ctaaaatttc atatgaaaat ttcgttgtac tgaatattgt atcatctggt aaaaaatgga
    26581 cagtcgtccc gtttgctttt tttgtttttc ctatcttttt caaagtaccg tcaggtttac
    26641 cgccgttttt aaatttctgc tgatactcta cgccatctct aacgatcgtt acaattagcc
    26701 atttcgataa ggcatttacc acactggctc ctacaccgtg cagtccacca gaagttttgt
    26761 accctccttg accaaacttt ccgccagcat gcagcacagt aaaaatcact tcaactgttg
    26821 gtattccgga agcatgcatt cctacaggca tcccacgtcc gctatctgca acagttatac
    26881 tattgtcttc atggatggtc acatcaatct cactgccata acctgataat gcttcgtcaa
    26941 ctgcattatc gacgatttca tagactaaat gatgcaagcc tcggctgtca gtggatccaa
    27001 tgtacatccc tggtcttttt ctaactgcct ctaatccttc caatacttgg atggacgagt
    27061 cattgtattc gttgtttact ttttttgcca aggcaaacgc tccttcaata atactttgcg
    27121 gcaaaaccgc accacgatta tcatactaga aaaggtcgtg aaaaaaaaga gggtagacga
    27181 gaaatcaatt ttaattatct cctaaccgct ttttcgacat tcccctctat cgatcatctc
    27241 ttctttgtgg tcatttttat ttttggtttt ctttcaactt accaagttca atcttaatac
    27301 aacgatccat tacgatatct gtacgtcctg cttttcttag catctcagct gcttctttgt
    27361 tttcaatccc taattgtgcc cagaacactt tagcatcagt tttcaaaaaa tcacctgcta
    27421 tttcaggtaa aaattcactc cgtctaaata catctacgat atcgattgga tcatcgattt
    27481 ctgtcaaatt cgcatagact ttctcgccta gaatttcttc accagccaat aatggattta
    27541 ctggtatgat tttatatcca ttgtcttgca atacctgtgc aaccatgtaa cttgttcgtt
    27601 ctggtttatt gctcaagcct acgacggcaa tattttttgc ttcttccaaa tagttgaaaa
    27661 tcgtttcttg tgttggattt tttacagaca tttctgccac cccttgcttt tctttttatt
    27721 ccatctatta tacttgtatc tttcattttt tcaattgttc ctgttaccta ttcatgctaa
    27781 aataaaggat agttttcaac ttgaaagagg gtaaaaaatg aaattgatct tattacttat
    27841 tttagcatat cttttaggtt ctattccttc cggtgtctgg attggcaaat tgtttttcaa
    27901 aaaagatatc cgacaacacg ggagcggtaa tactggaacg accaatacat ttcgtgtatt
    27961 aggcaaaaca gccgggattg ttgtcctgct tatggatatt ttaaaaggta cattggctac
    28021 ttgtttgccg atgatcttcc acctttctgg gattaaccct ctttggtttg gggtatgcgc
    28081 cattcttggc catacatttc cgatttttgc tgggttcaaa ggaggaaaag ctgttgcgac
    28141 aagtgcagga atgcttttag gatttaatcc tgtatttttt gtttattctt gtatcatctt
    28201 tatcgttagt ttattttgta caagtatggt gagtttgagc agtatgatca gtgcagtatt
    28261 gatcacttta tctacgatcg tccttcctta tgtagctcct gtgattttgg ctaagcccaa
    28321 ttggttactg acgatcattg cctttttagt ttcttccttt atcttttatc ggcataaaga
    28381 caatatcaag cgtatcagaa accatactga aagtcgaatc ccgtttggtt tgaatagcag
    28441 caagaaaaaa tcctgatact ttttcattcc aaagagataa aacgggagtg tgacaaatcg
    28501 atgtcacact cccttattac ttacaggata gcttttttac acaccttggt aaacggtgtt
    28561 ccctgctaac ttctcggtca caacctctta cttcactgtg atggaatatt ttgtcttgaa
    28621 gatttcatgg gcacctagtt gattgatccc tactttttcc atcaagtttc cactactgtt
    28681 caccgtgtca gcgattccgc accacggttc gatacaaacg aaaggcgctt cttttggata
    28741 aggagaccag atccctacat aaggcatatc tttatagctt aaagttacgc tatgggaaga
    28801 tttttcactt cgaatggcgt acgcatttag accccttgtt tcaaaaacca aggcatcttg
    28861 ctcaaaaagt tcacgattca aagccagatt cgtatttgtc tgacctaacg tcttttgatc
    28921 catatcgatg aatgggcctt tcaatggtag gcgtaatctt gatttcaatg gagaaaatgc
    28981 cagatagaaa tcttcaaatg acaatccttc ttcaagaggt acattgaaag caggatgtcc
    29041 accgatagaa aagaacattt ctttttcacc ggtatttgcc acttgataat gaaccgtgat
    29101 gccgtctcct cctaattcat agctgatcac taattcaaaa tcaaatggat acttttctaa
    29161 tgtctccggc gtactcctta agatgaaact agccatttct tgtccatgtt caatgacttc
    29221 aaaatccata tcccgagcaa atccgtgttg ccccatttga tatgttttcc cttgataaac
    29281 atactgatca tcttttaaac gtccaacaaa agggaataaa accggtgcat gtctgcccca
    29341 atacgctgga tcaccttgcc agatatactc taaaccatgt tcttttgatt tcagactgat
    29401 caattccgca ccgtgttcag cgatttcggc gatcagctgg tcattttgaa taataacagt
    29461 cattgccatt cctccaactt cgacttactt tttaaaatgg gaaggctgga acaaacattt
    29521 ctatctgccc cagtcccctt acttctaata actgtgtttt gctaactcgt ctttaaaacg
    29581 ggaattcaat acttttaaat aagttccctt catgcctaac gaacgagatt caatgattcc
    29641 agctgattcc aattttcgta aagcattgac gatcacagaa cgagtgatgc cgatactatc
    29701 ggcaatatta gaagcagtca gacgaccttc atctccatcc aatgcttcaa agatagcttg
    29761 tacagccttc aattcgctat aagataatgt gttgattgcc atttggacag ccgttgcact
    29821 ccgtacatct gcttcgatgc ttcgagactt ctgataaagg atctgcatac cgacaaccgt
    29881 agctccatat tctgctaaga ctagatcgtc atctgcaaac gattgttcca tacgagataa
    29941 gataatcgta cccaaacgtt cgccagctcc aaagatcgga acgacagtcg tcagaccaaa
    30001 tggatatttc tctcttgact ctaccggaaa tgcagtaata tcactagtaa ttgggatatt
    30061 agcttcagtt ttcatcaact gtgccactgt atcagtataa gattcaggaa attgtttctc
    30121 aacaaacatt gttttgattc gttcattgtt cacatcgtga cgttcattat aacctagtaa
    30181 tactccgtca cttcctatga tataggtgtt gctgtcaaga ataccaccta gtgtgacagc
    30241 cattttatcg taaggcaaat ctgcttgcat gtcaaaagta ttcttttgct gcaaaagtgt
    30301 gtttatcttg cgggttttcg ttagcaagtc tgtcattgtc gtcctccgat tttttataaa
    30361 atatagcgac ttaaatcttc atttgccgca attgagccaa gtttgtcatc cacatatgct
    30421 tctgtgatcg ttatttctcc catttgcata tctggtgctt caaataatag atcttctagt
    30481 agtttttcca aaatggtatg cagtctgcgt gcgccgatat tatctgtgtc acggttgaca
    30541 tcgaaagcga tttgagcgat ccgttcaatt gcttcttttg taaacgtaac tgacacattt
    30601 tctgtaccga tcaacgcgac gtattgtttt actaaagcat tatttggttc ggtcagaatc
    30661 ttcacaaaat cttcagctgt caaatcatct aattctaccc gtatcgggaa gcgtccttga
    30721 agttctggaa ttagatcact tggtttagac aaatggaaag ctcctgaagc aatgaatagg
    30781 acgtgatctg tttggatgat cccgtatttt gtgtttactt gtgaaccttc gacaattggc
    30841 aatatgtctc tttggacgcc ttctctggat acttctccag attgctgtga tttagaagtg
    30901 atcttatcga tttcatcgat aaaaataatt ccactttctt ccgctaattt gattgcctca
    30961 ctatggatat cagcatcctt gactaatttt gctgattctt ccttgatcaa taactctaaa
    31021 gcttctttga ctgttaccgt ccgttccact tttttcttcg gtgtcaaagc acctagagtt
    31081 tcatttaggt cgatccccat ttgctccaat ccattgttca tcattggtgc aggtttcttt
    31141 tggtcttcaa cttcaatggt tacttcacga ctatccagta aacccttttg tagttgttcc
    31201 aagactgtac ggcgattgat tcgaatatct tctgtcactt cttcttgcgg ttcagcggta
    31261 ttttgctgac tagcattaaa aagattcatc atttgttcaa attgattagt ggattgtttc
    31321 tgttcttttt tgataccagg taccaagatt tttaccaaac gtttttctgc atttttttta
    31381 gcacttgtgt atacacggct gtactgctct ttttctacga tttggatcgc attttctact
    31441 aaatcccgaa ccattgattc tacatctctt ccgacataac cgacttcagt aaattttgtt
    31501 gcttcgactt tgacaaatgg tgcattgacg atttttgcca aacgtcgtgc gatttctgtt
    31561 ttaccgaccc ctgtaggtcc gatcatcagc atattttttg gtgtaatatc ttgttgcatt
    31621 ttttcatcta actgcaaacg gcgatagcgg ttacgcaaag caacggcaac agattttttt
    31681 gctgcgtgct gtcctacgat atattcatct aattcactta cgatttgtct tggtgtttgt
    31741 tttaattcat acatatctac tcacctctta cagttcttct acgataatat tatgattagt
    31801 aaagatacag atatctgcgg caatatttaa cgcattttct gcaatttctt tcgcactcat
    31861 gtcgttttga gcatagtttt tcattgctcg ggctgcagcc aacgcaaaat tccctcctga
    31921 tccgatagct aagataccat catctggtgt gataacttct cctgtacctg aaacaagaag
    31981 catttcttta tcattcatta cgatcagcat cgcttcaagt ttttgcatag attgctgtgt
    32041 ccgccattct tgagctaatt cgacagctgc gcgttgtaga ttcccgttat attcgtttag
    32101 tttgccttca aatttttctt ctaaagtaaa cgcatctgca acacttccgg cgaaacctac
    32161 aacaacttct ccattgtaga tacgtcgtac tttctttgca gtgcctttca tgacaacaga
    32221 ttcacccatt gttacttggc catctccagc catagcaaat ttcccgtctt tttcgacggc
    32281 acagatagtg gttgaatgaa atggtgattc gaccatgttt gtttcctcct aaatttgttc
    32341 ttttccttat ttttacgctc ttggatggaa agaacgatag tttttttgta aactttcttt
    32401 tgttacatgg gcatagatct gggtagtcga taaattggca tgtcctagca attcttggac
    32461 tgtccgcaaa tctgccccat tatttaataa atgagtagca aatgtatggc gcagcatgtg
    32521 aggatggata tcactatcca aactgctttt tttgatcatt tgattcaaga catattcaat
    32581 tccagtaggc gtgattggtt ttccatgatg attcacaaaa acgatatcat gctcttcgtg
    32641 atattttgtc atcaatacct ggcggccttc cctgaaatat tcttgcagag catcttgagc
    32701 aaaagaacct agaggagcat atctctcttt gttcccttta ccatggatca acatgacctc
    32761 tgaatcaaaa tcaatgtctg ataccgacaa actgctacac tcacttaaac ggatgcctga
    32821 cccgtaaagg acttccagta aagcgcggtt cctcaaatcc aaaggactat ctcctgctac
    32881 gctgtcaaaa agtgcttgca tttcattttc ataaaaaaaa cgtggcagtc tcaaattttt
    32941 ctttttgaga tgaacgtaag aaaatggatt ttcttttatc acttcttctt tgagaagata
    33001 ctgataaaac gaacgtaggc tggctatctt gcgactgata gaatttctgc tatagctagg
    33061 acgatgatca gctaatgcac tcaaatagac acgaaccgta aaatattccg ccttcagcag
    33121 atcagcgtct ccagattcct ccaaaaaatg tttgaaatgg agaatgtctt cttcatatgc
    33181 ttctttcgtt ttttctgaat atcctctttc gacaatcaaa taacgcaaaa atttttgttc
    33241 ccacgtggct tctgtcattt tctcaccttc cttttgtccc agagaataat ttagcacagc
    33301 gtttataaaa agacaaataa attgtcagaa tttaaccaat atttctaagt ttgttcacaa
    33361 ttggcgcaat tcctcacatt ttgtttgaga aattcttaag aaaaacgcaa gaaaaagagc
    33421 gagactatcc gtctcaccct cttcatggag tttccttact catttgggat tgcagttttt
    33481 tctaattctg tttttattgc ctcattagct tttaaagctc gttgtgcaat tgcttcatat
    33541 cttgcttttt tgtctcgaat gcgctctggc agttcaggaa acagaccata gttggcattc
    33601 atcggttgga aatgttttcc ttccgcatga gtaatgtagt aagccatact tccaatagct
    33661 gtttcttgtg gaaactcgac tggttccatt tcttttgcta agcgtgcggc gttgattcca
    33721 gcaagtagtc cgctcgctgc actttcaaca tagccttcta caccagtcat ctgacctgca
    33781 aagaacagat cttcgcgttt cgttgattgg taagtcggtt tcagcaattc tggcgaattc
    33841 ataaagctgt ttcggtgcat cacaccatag cgaacgaatt cagcattttc taacccagga
    33901 atcatccgga aaacacgttt ttgctcgccc catttcaagt gagtctgaaa gcccacgatg
    33961 ttgtataaag aagctgcagc attgtcttga cgcagctgga taacagcata aggacgtttg
    34021 ccagttttag gatcttccaa tccaactggt ttcataggtc cgaaaagcat cgttttgaat
    34081 cctcgctgtg ccattacttc aattggcata catccttcaa aatatttttc tttctcaaaa
    34141 gatttaagtt ctgccacttc ggcattgatt agtgcctcat ggaaagcttt gaattcctct
    34201 tctgtcatcg gacagttcaa gtaagctgct tctcctttgt catagcggga cttcaaatat
    34261 actttatcca tatcgatcgt agctttgtct acgattggtg cagctgcatc atagaaataa
    34321 aaaccatctg aaccattgaa tgctttgatg ctttctgcta atggatgaga agtcaaaggt
    34381 cctgttgcta ctacggtgat tccttccggt atttcagtaa tttcttcgtt tatcactgtt
    34441 actaatggat gctctttgac ttttcgggta attgtttcag aaaaagaatc gcggtcaaca
    34501 gctaaggcac caccagctgg cactgctgtt tcatctgccg aagtgattac taccgaattc
    34561 aatcggcgca tttcttcttt caataaccct actgcattag ccaaactgtt tcctcttaat
    34621 gaattggaac atactagttc agcaaaatta cttgtgtgat gcgcctcagt cgatttgact
    34681 ggacgcattt catataaacg gacagggact cctgcttgtg cgatctgcca agcagcttca
    34741 cttccggcaa gccctgctcc aacaacattt accattttca atgaaagaac ctcttttcta
    34801 ttatttttgg atgtcttctt cgtaatctcc atttacacag actacttgtt taccgccttt
    34861 gactttcttc tcgaccagat atccgtcaca ttttggacaa tttctgccca ctggtttatc
    34921 ccaagaagtg aattcacatt ctgggaaacg ggaacaacca tagaatagtc tgtttttctt
    34981 agattttcgt tcaatgactt gtccttcatg acatactggg caagtcacac cgatttcttt
    35041 tacgattggt ttcgtattcc gacaatcagg aaaattactg caagcataaa acttcccata
    35101 tttccctaat tttatcacca tcggatgtcc acagacatca caatcgaatc ctgctggttc
    35161 atctttgatt tgaatttttt cgattttttc ttccgcattc gtcagttctt tttcaaacgg
    35221 tttatagaaa cgatcaacca ctttgaccca cgcttccgtg ccttcttcaa ttttatccaa
    35281 atctccttcc atttctgctg tgaaatggat atcgacgatt tgagggaaga attcacagat
    35341 taatgaattg acaatttctc ctagttcagt cggctcaaat cttttttgtg tcaattttac
    35401 atagtagcgt ctttggatcg tatcaagtgt tggtgcataa gtggaaggtc gccctacccc
    35461 gttttcttct agtgcacgaa tcaacgtagc ctcactaaaa cgtgctggtg gttgagtaaa
    35521 atgttgtttt ggttcaatat caacagattg gactacatcg ccttcttcaa gttctggaag
    35581 aatattttct ttctcttctt tgccgtcatc tctaccttcc acatacactt gcatgaatcc
    35641 tttgaatttg atttttgaac cgttcgcaat gaagatgaca ccattttgtt ctaatgtcac
    35701 tttcattgta tctaaaatag ctggtgtcat ctgactagcc acaaaacgtg accagatcaa
    35761 tgtataaagt ttcaattgat ctttatctaa atattgtttg agttcatctg gtgtacgaag
    35821 cacactcgtt ggacgcacag cttcatgggc atcttgtgcg ccttgagcat ttttgatctt
    35881 tcggtgatca tgtctagaaa attcttttcc atacgtatct tcaataaatg ctgcggcctc
    35941 tgcttttgct gtatcggcaa tccgggtaga atcagtacgc atgtatgtaa tcaagccgac
    36001 tgttccttgt ttacccaaag tgattccttc atataattgc tgtgctacca tcatggtttt
    36061 tcttgttcga aagtttaact tacgggcagc ttcttgttgc aggctacttg tagtgaaagg
    36121 attcgcaggg tttcttttgc gctctttctt ttctactttt ttaacttcga agtctgttcc
    36181 atcaattcta gcagtgactt cttttacact ttgtgcatct ggtaatttct ttttcttacc
    36241 gtcgattccc cagaaatttg ctttgaattt cttgcgagct tttttaaaat taccgtcgat
    36301 gctccaatat tcttctggaa caaattctcg gatctctttt tcacggtcaa taataatttt
    36361 taaagcgaca gattggacac gtcctgcact taaccctttc ttgaccttac gccagagaat
    36421 aggactgatc gaataaccaa cgattctgtc taaggtacga cgagcttgct gtgcatctac
    36481 taaatctaca tcgatcgttc ttggttcctt aaaagctgct ttgactgcgt cttttgtgat
    36541 ttcattgaag accacccgat ttttttcttt cagatccaac ccaagtaggt acgctaaatg
    36601 ccaagcaatc gcttctcctt ctctatccgg gtcacttgcc aagtaaactt tttgagcttt
    36661 ttttgctgcg gctttcaggc ttttgatgac atcgcctttt ccgcgtatag aaatataatg
    36721 gggttcataa ttgttttcaa aatcgatacc cattttactt ttcggtaaat ctcttatatg
    36781 gccaacactg gccattactt tatagtttcg tccaagatat ttctcaattg tcttggcttt
    36841 tgcaggtgat tctacaatca ctaaatattt atacgccatt actgaatgga ctcctttcca
    36901 ttgtcgtgtt cctactatta aaagaggatt catacaaatc gtagctaatc atatccattc
    36961 agaaaacgtt tgtcaagaaa agcaaatgaa aagtatggtg ttattcttta aattctgcca
    37021 agatatcttc acccgaaacc gtgcaaactg caccgtcttg gatcaaatga tgacatccgg
    37081 tagaatgttc tgttaaaata tttcctggaa cagaaaaaac tgttcttcca agatccaatg
    37141 cttgctgtgc tgttatcaga gagccgcttt ttttcttggc ttcaaagaca catgttcctt
    37201 tagacaaagc tgcgataatc cgatttctca ttggaaaaca atgctttttc gggccggatt
    37261 gaggaggata ttcactcaaa agaagatgat catttgcaat cacctcttgc aagtcacgat
    37321 tttcagcagg atagacttgc tctatccctg ttccaatgac tgcgattgtc cgtccaccat
    37381 gagagattgc tgcttggtga ccgtatgtat ctatcccttt cgcgcagcca ctgacgatca
    37441 catatccagc ttctatcact ggttttagta aataataaac tgtttttttg gctaaagcag
    37501 aagtgtgtct tccaccaaca acgcttaaca tgttacaaga aagtaaagat agatcaccat
    37561 tataaaacag tacaattggc ggataggtca agtgaagcag ctgaggggga tatttgggat
    37621 caaagcaagt gataaacgac tgtttcgatt ggattttgcc ccagttagcg tgaatttcct
    37681 gccaactttt gcgaaacagc tcccggtgtt tggttatttt acctatctta atgatgtctt
    37741 caaatccgat ttctttataa ggatactgca tcatccagtt agcaagtgcc cattttcctt
    37801 gactactgat tccttttgta taagcaagtt tcaataaatt ttcttctatt tgatacatca
    37861 taaaaccgcc tttcgtctct ctttctaata agatacgtaa aagcggctgt tttttattgt
    37921 aaatattttt ttacaggtgc aaaactttta cgatggattg gcgtaattcc ttgtcgttca
    37981 agtccttcta agtgttcctt tgttccataa cctgcattat gcgagaaacc atatccagga
    38041 taaagcacgt catattcttt catcaattgg tctcgatata ctttagcaat gatacttgca
    38101 gcagcaatcg acacagatcg tgcatcccct ttgattattt tttcctgctg aataggcaaa
    38161 tccaaggtca tcgcatcgat caacaaatag cttggtacaa tggacaattt ttctactgct
    38221 tggatcatcg ctaatttact tgcttcataa atattcacat catcgattgt ctgctgatca
    38281 acgatcccga tcccgattgc cagggctttt tcttgaactt gttgataaag aagttctcgc
    38341 tttttctctg ataattgttt ggagtcattc agccctaata tgatctcatt tttcggcaag
    38401 ataactgcag ctgccacgac aggtccagct aatggtcctc ttcctacttc atcaatgccg
    38461 gcaatccatt ggtgaccttg tgcgtaccct tgcttttcaa agcaaaacat ttcttcatat
    38521 ttttccttaa ttaatgcttc ctttttcaat cgtcgttcaa aagaagccag tgcttgctgc
    38581 acgcctttgc gtggatcatt tcttaatgat tctagtgtag gatcatctat tctctgtatg
    38641 cttttcaatc gttctttgat actttggatg gattcagtca tcactttttt gctccatttc
    38701 ttcgcaacga tccagtgtgt atcgtccaag ttttccttga cgaatatcat gcacgactaa
    38761 ttcgctgcca cgatcatagt cttctttata cccacgtttt gctgtaatat ccatcagtaa
    38821 ttccggtgcc aatttttctt cttgctcgat tgataaacca taacgatcgc tgattcttcc
    38881 tgaataatat cgggcaaaaa agtctaatcc gtaaatcgct aaatcatcta aatggacaag
    38941 ctggtctttg atcgcgcctg tcagcgctag ttttttgccg atttcttcat cttcaaattt
    39001 gggccataaa atccctggtg tgtctaacaa ttccaactca ttacctaaac gaagccactg
    39061 ctgacctttt gttacgccag gcttatttcc tgtctgtgca attttcttgc ctactagtcg
    39121 attcatcaac gtagatttcc caacatttgg aatacctaag accattgccc gaatagcgcg
    39181 aggcttgatc ccccgagcag catctcttgc ccgtttttct ttcaatgctt ctttagccgc
    39241 aggtactaga cctttaattc ctttactttc ttgtgccgtg atagctaatg caagatggcc
    39301 ttgtttacga aaatattctt gccacagctt tgtctgttgt ggatctgcca gatccgcttt
    39361 attcaataaa acaaggcgag gtttttgctg aatgatctga tctagcattg gattacgaga
    39421 agataaaggt aatcgtgcgt ctaccaattc aaatacgatg tccacatatt tcattttttc
    39481 agaaacttct cgtcgggctt ttgccatatg tcccggaaac cattggatgg tcattgttct
    39541 ctccattcct ttcataccgg aatattcccc ggctttactt gtttttttct ttttcaatat
    39601 aaattcactt ttttattata aagaaattcc atctattagt taagtgaaag tgaaccagtc
    39661 ctcattctat cacatcttca cttttttgat gatgcaaatt tttattttgg tttctatata
    39721 tgaatctaaa tttttgacag cgtttcctaa ataagagatg atagacgaac aaaacagaaa
    39781 ggagacgaaa caaatgaata gtatggtttt tgttaatttt cctgttgctg atgtcaatcg
    39841 ttccgttgct ttttatgaaa agctgggttt taaaaaaaat gaagaatttt ctactgaaga
    39901 agctggttca atggtatggg atgacacttt ttggataatg cttctctctc atgattttta
    39961 taaaaaattt ataaaagata aagaaatagc tgataataaa aaaacaagtg gtgctttgat
    40021 ttcgtttagt gttgaaaatg cggatgaagt aaaaaaattt gctcaaattg caaaagaaaa
    40081 tggtggtact ttccacgaag tagatatggg aatgcctgaa gaagaaatgt tttcattaga
    40141 agtccaagat ctagacggaa atacattaga acccgtatgg atgaagatgt aagcagataa
    40201 ctaatctgac gttttttata acaaaaaaac aaaggcggat gatacgataa gtattctccg
    40261 cctctatttt ttttattcgt acaatctctt ttttacagtt tataaaagca gtcttctcac
    40321 ccttttttta attgttagat gccagtgtat gtaaatcacc aatgtgtcat gacacattgg
    40381 tgattttatg gtatcctttt attattaact gacaaaggag agaatatttt gaacactttg
    40441 attgcaaaag acgtttttag attgcccgaa atgaatgcaa gattgttgtc gacctctgct
    40501 cttttattag cccttgatgt catcttatac aagatagcga ttggtcctgc ttattttcaa
    40561 atcacattcg gatttttaag tatggcgctt atgggttatc tatatggacc gatctgggct
    40621 ggtttgcttg aaatgttgtc gaacacttta aatttcacga tatttggttc aggaacgttt
    40681 caagctgctt ttcttatcac ggctttcctt ggtggattta ttaatggctt atttttatat
    40741 cataaaaaga taaacctgcc ttccgtcatc gctgcccaat tattgattat gatcccagtc
    40801 agccttttta tcaattcttg gctgatgtct gttttgtttg gtgcagattt caaagtaatt
    40861 tttctaggta gattcatcag gaatatgatc caaatcccta ttcaaataat ggttgtttat
    40921 tcatttatca aaactttaga ggctagaaaa gtgtttaaac gagctgtttg attttacgta
    40981 ctaaggtagt aaactgattt tggattaaac aataaagcgt gcagtctcaa cagactgcac
    41041 gctttattgt ttctcactgg atccatttta tttcatcaag tggataatag acaaatcttg
    41101 ctttacctag aatatcagtt ttattgatcg tgccaaacga acgactatct tttgaaactc
    41161 ttcgattatc tccaagtaca aaataattat cttccggaag cttggattgt cccgttagct
    41221 cttgtagtgt aaagtccgtc gtataggatg catttgcatg atctgatttc aggtttttag
    41281 ttagataagg ttccttaact actttcccat tgatcttcaa ttgatcattt tggtagctga
    41341 cattttctcc tggaagaccg attactcgtt tgatataaat cgtgccatct gctaattgaa
    41401 aaacaacaat atcaaatcgt ctgatctccg aaaatttttc catcacaacc atatcacctt
    41461 gcttcaacac gttttccatt gaattgcctt ccactggtac cgggatgaat aaaaatcccc
    41521 gcaacatgaa cgcaaggaat acagaaacaa gtaagtattt gaatacaagc cagaagcgat
    41581 taatatagcg ttgttttttt gtcaatctct atcaatccat ccctcttttt ttcattcttt
    41641 ctttattata agaaaaatat caaaaaaatg cgactagata gctgaaataa aaataattct
    41701 ttcaattagg ctattggaca aaaaactttt ttgatcataa agtacaaaac tatctcttat
    41761 atgtcatgat tattttctaa atatttttcc catgcttcgt caaagatatc catggacggc
    41821 aaatagtcga cattcagttc cagataatta gaaatttctt cgtagctaga gctgtgctta
    41881 ggaaattgga tgtctttacc ggcttctgta gcgaaaacag ataaagcatc atgtgtgcca
    41941 ccgcctcgca gtgtcattaa ataatgaaaa aagcttcgat tcacccttcg ttcctccaat
    42001 tttcttcgat aaaggcagcc cggcgtgcat ggtttctttt aaaattttca ggatgctttt
    42061 tataatactc ttgatggtct tcttctgcta gataaaatgg ttgggctggt tcgatttttg
    42121 taacgattgg ctgttcgaaa cgtccacttt cagctaactg tttcttactt ttttcagcaa
    42181 tgatccgctg ttcttctgtc aaataaaaga tcaccgggcg ataattgtca ccgcgatcct
    42241 cgaattgtcc gaaagcgtca gttggatcag tttgctgcca gtaaagttct actaattgtt
    42301 catatgaaat caatgttgga tcatagataa tctccactgc ttctgtatgt ccagtcgttt
    42361 ggctttttac ttgttcataa gtcggatctg gtacatgccc tccagtatat cctgacatca
    42421 ctgtatggat accaggaagc gtgtcgaaag gctggatcat acaccagaag cagcctcctg
    42481 caaatattgc tcgttccatt atgattcccc ctctaatact actcatccga tgatttttta
    42541 ttttcttttg tcttgctttc agtactgctg gtgctagttt tgttggtatt tgagttgttt
    42601 gaagcatcag aggtatcttt tggcaaatat agactaaacc gaatgtcatc atttactaag
    42661 tcgattttat ctgctttaat atacatcccg ttttgcattc ggaattgatc caacctaagc
    42721 atgatagtca gatcatctgt attgacttcg atccaatctg gtaatttata gtttctcttg
    42781 atcatattca tcacatcttt gattggcaaa ctcaatgttc cgatagaaag acttctagct
    42841 tttagttgga catttccgtt ttccatcaca tacggatcaa aatataaata gaaatcgata
    42901 ggaaaaccta gtacttcgaa agtaccattg agtagtgctt cattttccaa ataaaattta
    42961 tattcaatat cactatcttt ttgatactca ctaaggaaaa agctgatgat ttgattgact
    43021 ttttctttat ttgaattgat cgttaagaca ggatctcctt gtctttccgt aagagctggt
    43081 atttctggga gttctggttc ccgattagca aaaatacggc tgcctaaaaa tacggtcgaa
    43141 ccaagtacta ccccaagaag gataaggaag gcgaatttcc agtaatttgt tttctttgtt
    43201 ttgtttgaag atacgtcaga tcttgttttc atgtttattg atcatcctcc ttctctaacc
    43261 attggttttg tgtctcgatc agctgatctc tcactgcatt tgccatcaat tgataaccta
    43321 aattatttgg atggaaatga tcttcttcat agagcacatt atttttgatg ccatttagat
    43381 tttctgtcgt gctagttgtt tgatctgttc cttcttctgt gacacctact tcctgattca
    43441 atccttgata aagcaaatca ttgattggga taaagtagca gttcggcatt ttttttgtca
    43501 ccgtttctgt tcctttgttc caattgttaa caattgtctg catgtcagtg atctcaggaa
    43561 agttcaaata aaatggattg tagatgccta aaatgtaaat aggtgcggtt ttattgaggg
    43621 atcgaaattc ttccaatagt tctgtgacat tttcctgata gtttttcagt ggctttttaa
    43681 aggaattgac cgttaacccg aataaattat tttggataac tttcatcaaa tcattaccgc
    43741 caacagtcag tgtgataaaa tcagctgttt tgatattgtt tcgaatttct tcttttgttt
    43801 ttactcgctt taagatctgg tcacttcgtt caccatttac accatagttt tctgtttcaa
    43861 ccgcggtcaa attataacgg tcttttagat catttgcgac aaggggaacg aatcctcctt
    43921 gcttggtttg atctcctact ccttcagtca gcgaatctcc gatagctatg tagtgaagtt
    43981 tttctttttt atttggatct tctctcacag tagttactgg tttcattagt gggccagctt
    44041 tcggtataga agcagctaaa cctgcataga tgatgatcgc aattaataat gggaacaaaa
    44101 cgaggatcaa atgtcgtttc gttaatttca ttttttcacc tcttgtattc gattggttct
    44161 aatgttatca aaaattttat caaaaaggta gtctttatga aaaaagaaaa caattatttt
    44221 cctaatttta taaagaaaaa gagaataagc tgcaagcctt tcaggctgag ggcttatcct
    44281 cttttcgact aatctacata atacatgatt gcaaacgcgt ctttaccagt atgcgtagcg
    44341 ataaccggat tcgtatgcag tacagggata tgcatgtccg ggaataattc ttgcaaaccc
    44401 tctttgaatt cttgcgccaa ttcttttgca tccgcatgtg aaataccgat ttgtcgcaca
    44461 tttggcaatt ctttcagttc atttttcaaa ttattaaacc atttatcgaa cgttttcttt
    44521 cctcgcccct tagttacagg gattaattca gaatgggcga aattcatcac cacgcgtatg
    44581 ttcaaaacac tagacagcaa accagttgtt cggctgattc ttccaccttt taccaagtta
    44641 tctagtgtgg atattccgat gtataatttt gtattgtctt gtactttttt gacttcttca
    44701 aggattttct ctactgtttt accttctttt gctaattcag ctgctcgaat tacctgaaaa
    44761 gataatcctt ggtcagtggt atcactgtcg ataaccgtta ctttcgtttt tgttagatta
    44821 ctggcttgtc ttgctgcttc tactgttcca ctcaagcctt ttgtcatatg gatcgaaatc
    44881 acttcactgc cgtcttctcc tagctcatca tacatcgaga tgaattctcc aattggtggt
    44941 tggcttgttt tgggcagatt ttttgctttc gccatcattt ccataaatct ttcgccgtct
    45001 agttggtcat catcgggata tactgttccg tcgatcatga tggatagcgg catgacacga
    45061 atgcctaaag catcgcgtac atttttttcc atcgtacatg aagaatctgt tacgattttt
    45121 acatttgtca ttattttctc tctctctttc tttaacaaga tgccttattc atgttaaact
    45181 acatgtaaga caatttctat ttttagtata acagacaaat aatgattttt cattaattac
    45241 acctagtaaa ggagccgttt tcatggaaaa aacgaaattt aatcgaacgt atgaaatatt
    45301 aaatgaagtg tttaatgcag tcacccatgg aattgggact ggattgagta tcgcgggact
    45361 agtggttctt ttagtaaaag gtgcccactt gcattcctcc ctccatattg tctcttatgc
    45421 tatctatgga tcgatgatga ttcttttatt tttaacgtct actttgtttc attcattgat
    45481 ttttacaaaa gcaaaaaagg tgttccaagt attcgatcat agttcgattt ttcttttgat
    45541 tgcaggaagt tatactcctt tttgtttgct gagtatccga ggatggctag gttggacttt
    45601 atttgtatta atctggttgt ttgcgattct tggcattata tataaagcgt tgacgttgca
    45661 taaacgggag agagtatcaa aagtatctac gatcatctat atttttatgg ggtggctctg
    45721 tgtcgtggct gctgttcctc tttatcactc tcttggaatg actggtgtta ttttgatggt
    45781 agcaggcggg ctttcttaca cgataggcgc attcttttat agcttgaatt ctatccgcta
    45841 tatgcatgtt gtttggcatt tgtttgttat gttaggtgca ggttttatgt acttctctgt
    45901 tttattcttt acttaataaa aaaaataaag ctatggatgg gacagctcta aataacagct
    45961 agtctatcca tagctttttt atatcgtcaa ttcgcctgtc ccgcttttta caggattctc
    46021 attacgctga tacgtttcaa attgatgcgg ataacgattt ttttcatcca tttctccttg
    46081 actagtattg atcagtgacc aatcagacca gtcaacaggt ggaaaataag catctccatc
    46141 gaatacatga tggatgactg tacggtataa tatcgtacaa aatggcagaa attcttgata
    46201 aacagctgac cctccagcga tgaaaaagat accgtctgtt tctttttcca gagcaagcac
    46261 gtcttccaca ctatgcatga catgcacgcc ttctgcttta taggaagaat ctctcgtcaa
    46321 aacaatcgtc tgacgattcg gcaacggacg gctacccatc ccttcgaatg tttttcgtcc
    46381 catcactagt gtatttgctt ctgtcatttg tttgaagaat ttcagatcat ttggcaaacg
    46441 ccaaggaaga cggtcttctt ttccaatcaa tccgttttca tcctgtgccc aaattgcagc
    46501 taacattttc tcatcctttc tgattctaaa cagcgatagg agctttgatc gccggatgtg
    46561 ggtcatatcc ttctaattgg atatcttcca tctcaaaatc aaatactgac tgcttttcct
    46621 gattcaaaac taaagtcgga aatgcgcgta tttctcggct caattgttct ttcatttgtt
    46681 cgatatgatt gatataaaga tgagcatctc ctaatgtgtg aacaaattcg ccaaccgcta
    46741 aacctgtttc atgtgcaatt aggtgagtca gcaacgcata acttgcaatg ttgaacggaa
    46801 cgcctagaaa cacatcccca ctgcgttgat acaattggca gcttagccgt ccctcttgaa
    46861 cataaaattg aaacatcgta tgacaaggag gcaaagccat gctaggaaca tcttccggat
    46921 tccatgcaga aacgatcaat cttctagaat caggagtctt tttgattgct tcgatcactt
    46981 gtttgatttg atcgataaag ccaccgtctt ttgtttccca gtgacgccat tgatagccgt
    47041 agatgtgtcc aagatcgccg tatgtttcag caaaggaatc atcggttagg atcttctcgc
    47101 aaaaaagctt gctctgcttt ttatattctt cattgaattc aggatctgtc aaacatctat
    47161 ggccaaaatc agtcatatct ggtccattat agtctggact tttgatgtag cgttcaaatg
    47221 cccattcatc ccaaatatgg tttttatttt gtaatagata gcggatattc gtatctcctt
    47281 ttaaaaacca aagcaattcg cttttgatca atccaaaagg tactcgtttc gttgttaaca
    47341 aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc gaataagctt aatgtgcctg
    47401 tgcctgttct gtcttcttta cgatgccctt cttccaacaa ttttcttcct aagtcaagat
    47461 acgcttgttc catccttttt cctcctaatc ttctacccat tcactcaagt attcccaccg
    47521 tgccattttt tcttcaagtt gtgcttctgt ttcagatacg tcgttttgca attcttgtaa
    47581 tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg agcaatgaga ttttttcttc
    47641 aagttctgca atctcatctt caatcgtttc ccattctttt tgttccatgt aagataattt
    47701 cttcttttca gcaggttcct tgctctgtat ttttggtttc gctttttcag caggctgctg
    47761 ggtctctttt tgcttcagta aataactgga catgtttccg taataaagtt cttgttttcc
    47821 gtttccttgg aatactaata acttatctgc taccttgtcc aagaaataac ggtcatggga
    47881 aacagtgatc acagcacctg gaaaacttct aaaataatct tccaaaatcg tcaatgttgc
    47941 tatgtccaaa tcattggtcg gttcatccaa taataaaaca ttaggttgct gaatcagcag
    48001 tttcaacaaa aataaccggc gtttttctcc gcctgaaagt ttgcctatca gtgtcccatg
    48061 cataaatctt ggaaataaaa atcgttcaag taattctgct actccgattt gactgccatc
    48121 tgctgttttt gcttcttcag cagcttcttg taagtaagca atcatccgtt tatctggtgc
    48181 catttcttca ttttcttgtg tataatacgc aagatgaacc gtttcaccga tagacattgt
    48241 accgctatcc aggggaattc tgccagctag aatattaagc aatgtcgatt ttcccgcacc
    48301 atttttacca gtaataccta gtctttcccg agattgaatc aataaatcta aatgttctag
    48361 cagtgtttgg tcatttatac ggtaactgcc atctttgatt tccagtactt ttttccctaa
    48421 acgttgtgtc gctaggttca gctctaaatc atcttcttgg tttacttggt acaagttctc
    48481 ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg gtgccacgtg cctgtacgcc
    48541 agccctcatc caagctaatt cctgtttata aagccgcttg cgcttttctt cttgttctac
    48601 ttctacacga tcacgttctg ctttttctaa gacgtaagct tcataatttc ctttatactc
    48661 gtataatttt ccaaatgaaa gttcgaagat tcggtttgct actctatcta gaaaataacg
    48721 atcgtgggta accatcaata gtgcgccacg gtattgtttc aaatagtttt ccaaccactc
    48781 gatcgcttca taatctaaat ggtttgttgg ttcgtctagt aatagtaaat cgggttcatc
    48841 aatcaacact tgcgccagac taactcgttt tttctgacca cctgataaag tactgatttt
    48901 tttatctaat tcactgattc ctaatttttg caaaataatc tttgcgttgg tatctgtagt
    48961 ccaagcatct tctttattca tttgttcttc tgctttagca taacgttttt gcacatcagg
    49021 gtcttctccg ttttccccta gttcgatcaa tgcttcctca tatgctcgaa cagtttggat
    49081 caaggggctg tttccttgaa agactgcttg gagaacagtt aattcatcgg aaaattcagc
    49141 tgcttgagaa agataaccaa tacggtaatc agttggataa aacactgttt gccgatcacc
    49201 gtcgctgcta tcgataccgg caataatatt taagagactt gttttacccg tcccgttcgt
    49261 cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa gaaatctggt caaacaaatc
    49321 tttttcacca taagtttttt tgaggtcagt tacttttaat tctttcaaca tgttccctcc
    49381 ttatcttgga tattgtatca taaaaatagt gaacttcagg aaaatttttt tggtatttgt
    49441 tctttttgtg ttctcaaaaa aagaacagga ccgattcgat cctgttctga tatgttttgt
    49501 tttttataaa acttgaaaat ttttaccttt ttattttatt ggactcgttt tttttgactt
    49561 tgctcgaaac gcaaatcgga aagaaaagtt atcagatggt ttgttcatcg tataagattc
    49621 taacaaattg atattcctgc gatagctgtt cgcagaaaac gtcgtgattt tttcagcaac
    49681 ttcgtattca tcaatgattt ttctttcaag ataataacct cttagcattt ttttcgtaaa
    49741 cagactatct tgctggatca tccacgaaga aatcaaattc cccgagttca tttttattcc
    49801 ttcaagtttg cgttgctgga taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa
    49861 gttatctaaa ctttcaataa tcaactgagt atttttttga tatgtctctc tgatttcttg
    49921 gatatcttca tcactaataa atgtctgacg atcagcatat cttctttgcc aaaacatttt
    49981 cggatggaga acgattcgca ttaacctacg gacaacaatg aaccaaaagc cgataaaaga
    50041 caaaatctgg cttgtgatag aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata
    50101 accattagaa gaaagcttgc ccgaacgata actttcatcc aatccatccc gttcgatcga
    50161 caaaatcagt gcttgtattt cttgtacttc ttgcttttca tcatcggtta aatcttccaa
    50221 gtacagatca cgtatccgtt cttgataatt ttctatcact gcttcagtgg ctaaaaattc
    50281 ttttgtatta cgatcaattt cctttatttc ttttcgcaaa acttcaacga cctcttctaa
    50341 aatcatcaaa gcattcaaat ccgtgctttt cgcttcctct gtttccgtca gcataggcaa
    50401 gacaatgatc cctatgacca aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa
    50461 agaccgctgg taaaacatca tattattgat tgaaaatggc aaaataaaaa tcgtagccaa
    50521 actgactgtt ccctttacac cgccaaatgt taagatcaag atctcgttca actgtttttt
    50581 gaacttttta ctgccatctt tgaatacata aaaaagactg atagaaatga atctgatcac
    50641 aaaaagcatg acagagatta atacgataat cgctaataaa agaccgtttg gatataaacc
    50701 gtctccccat actggcgaga acacttgcgt caattcgatt cctaaaaata taaatactaa
    50761 tgcattcagt gtaaaagcaa tagttgtcca tgtactgtgg gacaagctcg acaattctgc
    50821 atcaaacaca gagattttcc taaaaccaga agcttggaga atacctgctg ccactgcagc
    50881 aatgattcca gatacatcaa atgcctctgc caatacatag gctagaaaag gaagcaaaag
    50941 ttcgatcagc agataccctg tgacatcttg tgctgatgct ttttcgatca aatgaattat
    51001 tttttgtttg aaccagacaa gcaaaaagcc gatcaaagct cccccaatac tagaaactac
    51061 tagactcatc cccgcgttta ctgctgaaaa agatccggtg atcagcgcac caagagcaaa
    51121 ctggaaggca gtaacaccag atgcatcatt caaaagacct tctccttcca aaatatgcat
    51181 cgccttttta gggatattca ctcgtccaga aagagaactg actgctaccg catctgtcgg
    51241 acctaacgct gctccaaaag caaagcaggc agcaagtggg acacttggca acaggaatga
    51301 taacgtcgca cctaccccga caagggtcaa aatgactcct ccaaatgcca agaacaaaat
    51361 tgtcccgaaa tttttgagaa tcgaagaaat atctgctttt tctccttctc taaataacaa
    51421 tggtgcaata atcataacta agaacaattc aggttcaaaa tcgattgatt gtccccactc
    51481 tgtcagacct aataccactc caaggaaaat ttggatcagt ggagctggaa tcattggcaa
    51541 aatgcggcta gctatgttcg ataaggtgat cgttactgca aatacaataa ctaactcagc
    51601 aaattccatc gtccatcccc tttatttttt attttgtgta agcattttta aaaattgttc
    51661 ttttttcttt aaaattttgg tttcgactac ttcaacctgt gtttggttcc cctctaattc
    51721 acactgtaca cgttttgact ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg
    51781 atacatcaat atatttcttt tacgcaactt acaaagtatt tgacggcggt catccatcga
    51841 aagggcgcca tagcgatcat gcaacagttt tttttgttgt tcaaaactct ctctcatttt
    51901 ccagatctcc tatacacaaa cttccatcat tatagcaaag ttctttttgg atgaaaaata
    51961 tttcattgtt tcatcgttta ttgctagtta gaaatttcca taaatttttc aacgcctcat
    52021 tctattccaa atagtatcag atatcaagta aaacttaaga aagataaaag tttctgacaa
    52081 aaggatgttt actttcccat tcctgccttt cttgttcttt tatcacaaca aaaaaatggg
    52141 gctgcgacaa aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc
    52201 ctatcagtag taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt
    52261 attcgaaaaa ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca
    52321 agttacctgt tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc
    52381 ctttatctag atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca
    52441 ttcgctcgat atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta
    52501 ggttgatatc atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag
    52561 cacacggatg gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt
    52621 gtttgatcca aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa
    52681 aataaaattt tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt
    52741 tattcttgta ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt
    52801 tcttctaatg acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta
    52861 gtgtttttta aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt
    52921 ttattctttg ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac
    52981 caggtttttt tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg
    53041 acttgatagg taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa
    53101 aatagatcat caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga
    53161 atggttccaa agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt
    53221 taatcatttg gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta
    53281 gatccagttg gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg
    53341 gtaattcagc aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt
    53401 agcactctgt ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct
    53461 tgatgaattc cacatttata cgttctacag atattttacc taatagctcg tggttttctt
    53521 tgatggcgta gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc
    53581 cgcgcatcat tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca
    53641 agatctgttc ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt
    53701 tcaaagcaaa cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa
    53761 cgaattcgac atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg
    53821 tgatctcata ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat
    53881 ctatcgtttt tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca
    53941 catcatggat tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt
    54001 catatcctgc attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta
    54061 attgttttaa tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact
    54121 cagttgcatg gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc
    54181 atcttgttta ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca
    54241 tgtttttttc aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa
    54301 cgaataaaac atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc
    54361 ttttttctcc ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc
    54421 gagccatctg agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca
    54481 cttcctgctg catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat
    54541 cttaccaaaa ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg
    54601 ctacatttag gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg
    54661 aattcatatg gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag
    54721 tatttatgca tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt
    54781 ctcagggatc accttgatct taagagcttt atttggtatt gatccagctt atacgacttt
    54841 attaatcaac atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc
    54901 ctatactgtt ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc
    54961 actagaaatc aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc
    55021 aggtgtagga agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat
    55081 cgctcggatt ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga
    55141 tatcatcgtc ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt
    55201 gattgtttcg tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc
    55261 aaaaggtcta cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg
    55321 tctgcaaaga ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa
    55381 aatcatctat atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga
    55441 gattgacgaa aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt
    55501 tacttatttg aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata
    55561 ggctatgagg aaagattcca catccagctg acctttgtct tttttagtac tatactattt
    55621 ttaactaatt gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct
    55681 ttcggttaac ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt
    55741 gatcgatttg agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc
    55801 gttcaattaa ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt
    55861 cttctaagat agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat
    55921 ataaaggctt tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt
    55981 cttttccctg caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca
    56041 gtcctcgatc cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat
    56101 gattggcaag ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga
    56161 ccatccacgt taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc
    56221 tgttaggtca tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata
    56281 agtcaataat tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc
    56341 tttcaagaaa tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc
    56401 aacggtaaag tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt
    56461 agcagtttct tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa
    56521 gtaaagatta cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt
    56581 cagtaaaagt tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag
    56641 gtgataaagt tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc
    56701 ttcttcaatc atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata
    56761 atgcggatta acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa
    56821 ttgaagattc gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc
    56881 ttcttctaaa ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa
    56941 acgttcctgc atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata
    57001 aaccactgct gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg
    57061 gatcaatggt tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc
    57121 agggattccg ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc
    57181 actttgctta tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat
    57241 tgcaatttct gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg
    57301 ttcaaaaatc tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc
    57361 agctaatata tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga
    57421 caagtcttca tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc
    57481 ctcttatctc actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt
    57541 ttggatgttg gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt
    57601 tttctgccaa aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt
    57661 ggattggata gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag
    57721 gatatgcttg gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag
    57781 cagaaagtag atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat
    57841 ttgacaggtt ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa
    57901 acggccgagt cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac
    57961 gatgatcaca aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca
    58021 cctgccgctc gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca
    58081 gcactacacg atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag
    58141 aacggatcga tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa
    58201 aacctgccag atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt
    58261 cgggatagcg tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag
    58321 tgttggcaca aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat
    58381 ctgcaaacaa atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa
    58441 gttctcttag ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta
    58501 cttgataatc aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa
    58561 gcaaattcta gaaaagataa gagcaaagct cacctcataa cttgcaaata aataaaaatt
    58621 aaataaaata catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt
    58681 tattcctttt ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc
    58741 tttttcaact gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta
    58801 ctttgctagc tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg
    58861 cgcgttcgcg aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta
    58921 aagcatcttg aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta
    58981 aatcagtagc tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca
    59041 cctcctccac aagagatatg atagaaatat catttagtta aaactttctg agtggtccag
    59101 aagtttaaaa agaaatattg attaacttca atattctgtt atcacgatat catatgaacc
    59161 gcttaatagc aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca
    59221 aacgaaactg tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct
    59281 cctacgaggg ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt
    59341 ttctaagaaa cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa
    59401 tgtaggtggt ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc
    59461 tgttggtgtt ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga
    59521 gatacgaaga ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg
    59581 ttgtttagtc actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc
    59641 acgaatctct tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt
    59701 attcacgacg ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc
    59761 ttgttcccga atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat
    59821 ggcacgcatt gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt
    59881 acgcatacca gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt
    59941 gtcgattgcg tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa
    60001 aatcgcattg atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat
    60061 cgtgtcgtcg tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat
    60121 atcaccaatc cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga
    60181 ataaaattca tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat
    60241 cactggttta ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc
    60301 ttcgcgtccg ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg
    60361 atgcttgatc tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac
    60421 gctgaattct cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg
    60481 agtatcttcg acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc
    60541 gacgttcggg cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa
    60601 gtttccaatc tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat
    60661 taaacgaagt atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag
    60721 acaaaagcag ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat
    60781 cggttgcttt ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa
    60841 atccagtatt ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag
    60901 ctggtttttc ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct
    60961 ctaatacttt cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat
    61021 gtttatgtga aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa
    61081 aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt
    61141 ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt
    61201 cttgttcagg attgatagac aacactttga catttacatc atcattgaca gccaatacat
    61261 cacttggttt tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc
    61321 cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat
    61381 ctccctcatg gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta
    61441 ccgctttatg cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc
    61501 tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg
    61561 cagggacaaa accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa
    61621 caggtgcttc gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt
    61681 tagcatctaa acggcgtttt gaaagtagat agctgccgtt ttctttatct ttgccgatcg
    61741 tactgataac gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta
    61801 ccggcaaagt tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc
    61861 cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt
    61921 ttacttcttg tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat
    61981 caaattctgt cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt
    62041 tttagtttaa ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa
    62101 caattgtttt tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac
    62161 agcttttatt gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc
    62221 atcctctgct tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg
    62281 tatctctttc gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa
    62341 acgtctttct gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg
    62401 taatacagct gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt
    62461 tcgttggaca gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc
    62521 ttttgtgaca tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg
    62581 ctgtccatct tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc
    62641 ttcttcatct gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat
    62701 ggcaccggta tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact
    62761 tttcccagat gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct
    62821 ttttctcaga aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc
    62881 agggtaaagc ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt
    62941 gatccctgca cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga
    63001 accggctgca gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc
    63061 ttgataagtt gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga
    63121 cgttacacct gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt
    63181 tgaagtctca gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga
    63241 agtttcagaa gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata
    63301 agcacctgct ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa
    63361 tgtatttcct ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc
    63421 gtagattggt tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc
    63481 ttttctgctc actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag
    63541 tgtcttgtcc taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt
    63601 tcccaatgaa gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt
    63661 gctccatcaa tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct
    63721 aagatgaatt ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg
    63781 aattcttttt ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac
    63841 tgctggatca gctccatcca ttttttttgc agctcatcaa agggagcttg ctctgtggca
    63901 tattccaaat aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa
    63961 tggatacgtt catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg
    64021 cctgctcgac cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc
    64081 acataacgga tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac
    64141 tgcagctgat tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca
    64201 ccgtgatagt atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct
    64261 ttttttgttg cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt
    64321 tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat
    64381 tgtctgatct cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa
    64441 tctgttgctg tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt
    64501 cctaactgtt gatattctgg acggaaatca actccccatt gagacacgca atgagcctca
    64561 tctactacat ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc
    64621 aacatttcgg gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt
    64681 ctttcagatt gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt
    64741 aattgcgtca cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct
    64801 tccattaaat accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata
    64861 gcgagagtat cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat
    64921 gaatcaaatc caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct
    64981 caatagatag atttgataca aacgaaattc tccataatct aattgtccat ctacattcca
    65041 ttcgctaaag cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc
    65101 tgcagagaga atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg
    65161 atctgttact gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc
    65221 attcataaac atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga
    65281 atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg
    65341 gatacatctg ctttgttgca gataaaccgc tgactcatca ttgttttttg ccagctgata
    65401 aggaagcagc cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc
    65461 ttctggtatc tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc
    65521 acgtggcaat tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt
    65581 ctctgctgga agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa
    65641 tttaatgagc cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt
    65701 atgttctatt tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt
    65761 ttcatttatt tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc
    65821 ctgcttcaat tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat
    65881 aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt
    65941 gtgtcctgat gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga
    66001 tggttagaaa tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt
    66061 tgtcaaatcg aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc
    66121 caagaaccag aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa
    66181 gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg
    66241 aaactttaaa aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc
    66301 ttgttccgtg aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac
    66361 tagttttttc acggacccta taggacccat ttatattatt tcaacttttt cagccagttt
    66421 tccaatgtat tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct
    66481 cctttttgct caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac
    66541 tctgttcttt ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta
    66601 tcttttgtcg ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg
    66661 tcgggataac ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg
    66721 acataacgac ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt
    66781 ggaaaatctt ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc
    66841 attttgacaa tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa
    66901 ctttctggct ctacgagagc accaggataa aaaataagta atggagactt tccatgattc
    66961 tttttctctc cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact
    67021 tgcgcttctg acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg
    67081 agtaatccta aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc
    67141 caccttttct atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa
    67201 taagagagaa atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat
    67261 atccgccctt tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat
    67321 aaatttccat tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa
    67381 agtcaggagt atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa
    67441 aagtagcgcc gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc
    67501 ccaatagatt tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa
    67561 attcttcata agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat
    67621 ctttcttgaa tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga
    67681 tttccaaact tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca
    67741 cgacagcttc accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa
    67801 aatcaaatac ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg
    67861 catctttcgc gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat
    67921 cgaattcgtg ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag
    67981 ctgcatcagg aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg
    68041 gtcctgtcaa gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat
    68101 ctggcgcaaa tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc
    68161 ataaattttc ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag
    68221 catcaatcaa agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg
    68281 atccgcgacc aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat
    68341 tattttcgtc ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc
    68401 cagagccaat ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta
    68461 gcttttcact gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat
    68521 gtccttgtcc tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg
    68581 attctaatgc ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct
    68641 aatatgttaa aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg
    68701 acgttttaag cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa
    68761 cactcactga atggagctat tgacttatcc acgtgcttat cgatagccgt ttatcggttt
    68821 cttcattctt atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac
    68881 acctaggatc acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca
    68941 ccacactgaa acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg
    69001 tgtaattaag tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc
    69061 gacgccggca aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga
    69121 agttgaagga atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac
    69181 acttgaagat aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa
    69241 tccattgttc ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat
    69301 caaatcagaa gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt
    69361 gattgctact acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc
    69421 acctaatgga agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca
    69481 gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact
    69541 gatgatcatg ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt
    69601 gattacttct tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg
    69661 gttgtatact aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata
    69721 tacaatagaa atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc
    69781 catcgaaatc ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc
    69841 tagacagcct acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc
    69901 tacagccatc atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc
    69961 gtaagtagag ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt
    70021 tcctttgaat ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat
    70081 aatacctaaa aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt
    70141 ttctttagtt agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat
    70201 atcccacagc ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc
    70261 tgcatcgtct acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc
    70321 aataataata aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg
    70381 ttccatcttc ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt
    70441 ttatcaaaaa gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa
    70501 aaagttcgtt tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt
    70561 cattgtagcg tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat
    70621 ttcacatttg caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa
    70681 acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag
    70741 aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt
    70801 tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg
    70861 atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac
    70921 atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg
    70981 aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa
    71041 aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta
    71101 aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct
    71161 gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg
    71221 aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa
    71281 gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct
    71341 actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct
    71401 cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat
    71461 agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta
    71521 tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact
    71581 ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt
    71641 tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa
    71701 gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat
    71761 ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca
    71821 caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg
    71881 tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg
    71941 gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg
    72001 tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca
    72061 ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca
    72121 gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc
    72181 ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac
    72241 cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt
    72301 ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt
    72361 cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca
    72421 ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc
    72481 aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata
    72541 atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc
    72601 tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc
    72661 tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt
    72721 ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg
    72781 agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc
    72841 tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc
    72901 ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat
    72961 aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat
    73021 aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca
    73081 attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag
    73141 aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt
    73201 cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac
    73261 gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca
    73321 acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac
    73381 ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta
    73441 aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga
    73501 cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca
    73561 ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat
    73621 attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca
    73681 gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca
    73741 actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat
    73801 ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa
    73861 gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc
    73921 gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa
    73981 gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt
    74041 tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt
    74101 ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt
    74161 ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc
    74221 cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt
    74281 ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac
    74341 taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac
    74401 aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg
    74461 ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc
    74521 aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc
    74581 actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa
    74641 cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact
    74701 tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat
    74761 acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt
    74821 tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc
    74881 gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc
    74941 acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag
    75001 ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc atatcagata
    75061 ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact
    75121 cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt
    75181 agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt
    75241 aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg
    75301 aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact
    75361 ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca
    75421 atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc
    75481 tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct
    75541 tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca
    75601 accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat
    75661 tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt
    75721 ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct
    75781 taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg
    75841 taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat
    75901 ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga
    75961 caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag
    76021 gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc
    76081 ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag
    76141 atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa
    76201 gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat
    76261 caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt
    76321 ttcgtgtagc agtaacgatt ccaaaaccgt aatgaccgat acggctttta ggaatttgtg
    76381 tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga
    76441 ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt
    76501 agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca
    76561 tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacata
    76621 atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa
    76681 cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc
    76741 tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga
    76801 tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg
    76861 ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct ttttctaatc
    76921 tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga
    76981 cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag
    77041 atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct
    77101 gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg
    77161 cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt
    77221 cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact
    77281 gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt
    77341 cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag
    77401 ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc
    77461 caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt
    77521 catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga
    77581 tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt
    77641 cattcttaag actggataaa tcatttttta gttttgataa atcttgatga atcatatctg
    77701 cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt
    77761 ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt
    77821 cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta
    77881 ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt
    77941 gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct
    78001 taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag
    78061 attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg
    78121 tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta
    78181 attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt
    78241 ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct
    78301 tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt
    78361 tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat
    78421 tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt
    78481 caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct
    78541 tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg
    78601 gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc aattccgacc
    78661 attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct
    78721 gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag
    78781 ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat
    78841 ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa
    78901 tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg
    78961 aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gctttttttc
    79021 ttttaaaata aaccaaacag ttttactgat ttctcttctg gtcaatagtc cacgcttatt
    79081 ctaagaaatc ttttaattgt tttgaacgag aaggatggcg caattttctt aatgctttag
    79141 cctcgatctg acgaatacgt tcccgagtaa caccaaacac tttccctact tcttccaatg
    79201 ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag gacattctct tcccgatcag
    79261 tcaatgtatc tagtacatct tccaattgtt cttttagtaa ctcataagct gcgtgttctg
    79321 ctggacttgt tgcttcctgg tcttctatga aatcacctaa gtgagagtca tcttcttcac
    79381 cgattggcgt ttcaagggaa acaggttctt gcgcgatttt caagatttct cggacttttt
    79441 ctgttggaag atccatttcc gcaccgattt cttctggcgt aggttctctt ccaaggtctt
    79501 gcagcaattg acgttggata cgtatcaatt tattaattgt ttcaaccata tgaactggaa
    79561 tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga atccaccatg
    79621 tagcataggt tgaaaactta aatcctttgc ggtagtcaaa tttttcaact gccttcatca
    79681 gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct acataacgtt
    79741 tagcgatact tactactaaa cgaaggtttg cttctgccag acgctgtttc gcttcttggt
    79801 cgccttcttc gattttcaat gctaacgcta cttcttcttc cgcagttaaa agggagacac
    79861 ggccaatttc tttcagatac atacgaaccg ggtcattgat ttttacaccg gttggcgcag
    79921 aaagatcttc tgtttttgct tgttcagctt ttttctcagc actcttcaag ctgtgaattg
    79981 atggatcacc attttcatcc accacactga tacctgcgtc ttcaatcttt tggatcaatt
    80041 tgtccatttc atctgcattc aaagtgaatg gtgtagcaag ctgattggat aaatcatcat
    80101 atacaacttg tcgtttgggt ttgttttctt tgatgaaagc tgctactgct gcttcatacg
    80161 tttttttatc tgttccattt gccataaaag aaggcccccc ttgagtagct atttggcttg
    80221 ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa gctgtttgtt
    80281 tccagttcgt ctggcttctt gctgcatttg tttttttaat gttatttcat cagctaagcc
    80341 agatttgctg atcacatcta gcaagtcttg gatttctcgt ggcgaacttt cttctgccac
    80401 cggtaaatat tcgatttcca ccaccaaccg ccttaaattt tcatcttgca aaaagtctaa
    80461 aaactctgca aggttgaatt cataatgttg tgagacatat gcatcgaata aaaaataaac
    80521 ttctgcatat gcatcatgaa taaactgaaa ttctcgttct tttaaaatat tacggacaga
    80581 aacttcttta aatgcacgat ataatagaat ctgctctgcc ttttctactt gcgtcaaagg
    80641 tctctttttc cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt cctgaggttc
    80701 ttgacgattt tgctgatttg tccttcgaag atcgcgcagt tgtttttgta tcgtctctct
    80761 gccgatctga aactctgttg acagttgaga tagatacata tcttgttcga ttggtgaaac
    80821 aactcggcta agttcaacta gtaattcttc gaggtattcg atcttgtctt tttcattatc
    80881 taaatttttc ccttgtttta aataacgtga tttaaaagta aagactgttt cgcgcccatg
    80941 agccactaat tcttggaaag atgcttctcc atacttacgg agatattcat caggatcaag
    81001 cttttctgga acgactacga cagaaagtgc taatcgactg ttttgccgaa gcaattcaat
    81061 ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga ccaattcttt
    81121 cgtcagcttt tccatttgtt ggatctgttg agctgttaga cttgtcccca tagaagctaa
    81181 tccagttttg ataccagcct gccatgcagc aatcacatcc ataaaccctt cgaacaacaa
    81241 aatttctccg tttttccgga tatcgcttct tgctttatca agattaaata aaacttgtct
    81301 tttattgaac agttctgttt ctggactatt caagtatttg ggctgatctt tttcctgccc
    81361 tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc tggccttgag aattacgaag
    81421 tggaaacatc actctttgat aaaaacgatc agacatacgg ccatcatcat gcgtgacaaa
    81481 caatccagag cgttctaaca attcatttga aattgattca ttcttgaata cttgctgaag
    81541 aaaagaacgc tcattaggtg caaaacctat gttgaattct tcaattaatt ctaaagacaa
    81601 cccacgactt tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca atagcatgtg
    81661 atgatagatt tccgctgctt tttcatgagc agcaatcagt ttccccgtag cagattcagg
    81721 attttggaca gctatagtac gctgacgcca ttgatcttct aacggtatct gttcgaagtc
    81781 tgccactttt accactgctt ctggaaaact tagaccttct agttcttgaa gaaaagtaaa
    81841 cacgtttccg cctttaccac aaccaaagca atgaaaaatc tgcttgtctt ctgcaacaga
    81901 aaaagacggt gtacgttctt catgaaaagg acacagcccc aagtaatttt taccagactt
    81961 tttcaactgg acatattgtc caatcacctc aacgatattc gtttgactgc gtactgtttc
    82021 gataacctct tgaggaatcc gttgagccat tttttcaccc cctgctctat tgatatcttt
    82081 tgggtttaac agaagatatt tcaggagaat aaccggtaaa aaatcgcact aactgaaatc
    82141 agtgcgattt taaaggacac aattatacat gtttgattgt atcatatttc attgcttttt
    82201 caagtataac acatttctat ctcattaagc atcaatttgc tactcttttc agtaactgat
    82261 tttgaatatc agccttccag tacaatactc gaagaacaat atttacgagc atgaatacaa
    82321 agaagtatcc tacgctctct cccaacccta agtttacttc caaaattcca tggaacagca
    82381 ttgcagctac atatatccct acgatcgtcg ttactgctag gaagatgcgc aatgggttga
    82441 agggataaca agcttttaca actgccatgc aactgatccc aatcagcaag taatacatca
    82501 aagtagtagt ttcagcattt ccccatgcga atgtttttcc aagtagatac accgcaatga
    82561 tattcactac tacaagcaag gcatttggta aagcattaat aagtgctgta ggcaaataac
    82621 ggtaagtgat ttttctttta tcttgttcaa aagataaaaa gaaggaagga tacccttcaa
    82681 tcgctaaatc aatcagcgtg atttggatag ggataaacgg aaatccaatt gctgtaacgg
    82741 cacatataat cgataaaata aatgaataaa tcgttttgat aaaaaagatg cctgagactt
    82801 tcgtcacatt gttgaccact cggcgtcctt caaacaggac ttctggcaat gtagtaaaat
    82861 cagagtccaa caataccaga ttcgcaatct gacgtgttgc tccatcacct tctgccatag
    82921 caatgctgca atctgcttct cttaacgcca aaacatcatt cacaccgtcg cctgtcattg
    82981 caaccgttct gccgctttct ttcaattcat ttaccagcaa tttcttttgc tgcggactca
    83041 ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac ttccatttct tcagtaattt
    83101 gagaaagatc gacatacgca tcataattcg gtaatcctgc acgtctagcg atattggaga
    83161 cagtaaaagg attgtctcca gaaatcactt tcagatccac accttcctca tgaagataag
    83221 ctagtgtttc ttgagcgttt tttcggattg gatcatcgat ttcaaacaga gcaaccggct
    83281 caacaaatgc tggtttttct tcgttcaatt gctgatttgg agcgatcccc aacataagta
    83341 cgcgatagcc attcgcttgg gctgttttta gactgtccgg aagccgatct tcttccacca
    83401 aacgttcagg cgctcctacg tacacgactc ccagttctgg aaattccatc gcgccccatt
    83461 tgcgatcaga agaaaatgcg actttgtttg tcacatcata ccgattagaa agctcaaaat
    83521 aatctctcat agcccgcata gtgatgttgt tatcactgct ttctgccaga tacgtcccca
    83581 taatttctgg gatggctttc tcgtacagcg gatgtagtgc ttctacccgc tggatactca
    83641 ttttcccttc agtaatggtc cctgttttat ccaagcataa tgtatcgaca tgggccagcg
    83701 tttcaattga atacatgtct tgaacaagga ttcgtttttt tgccagtttg gttacccctg
    83761 tcgttaatgc aatacttatt agcaatacca aaccctttgg aagcatgcct aacaaagctg
    83821 ctgcagatgc aataacagaa attttgatag aagcatcgcg gatgaacagt gcttccaaaa
    83881 atagtatgat ccccaaagga ataatcacga aactagtgaa tttggatact ttgcgaatcg
    83941 aatctacaag ttcagaacga atagctttgt gagtctttgc ctcgctagtc aagcggacag
    84001 cataattatc tgcacctact cggataactt cagccatgac agaaccgctt gtcaaaaagc
    84061 ttccagaaag caattcagct ccagtttctt tgacaatcaa atcagattcg cctgtcaaaa
    84121 gagcttcatt tgcttctgcc cgtccagata taacctgtaa atcggctggt atctgttctc
    84181 ctgctccgat ttttaccaaa tctcctaata ccagctcttt agtagaaatc gattgttctt
    84241 taccgtcccg gatcactagt acttctgatt gggaaacaat cgataatttc cgtacaagat
    84301 ttcttgcatg tatttcttga taaattccaa taacgatatt gacgacaata attgctaaat
    84361 acgccatgtt ggaatacgca cctacaaata acagacaaat cccaatacca aagttcagaa
    84421 aattgaataa cgtcaatata ttatcgctga atatttgttt tgtacttttt gatgcatctt
    84481 cctggtagtc attttgctgg ccagaagcca ttttctgaac tacctcttgc tgtgtaagtc
    84541 cattcatttt ctttttctcc attttccgcc aactttcaaa aataatcacg tgtttaagtc
    84601 tagcgtgttc caagattaaa aacaacttgt ttttataaaa tttatagaaa aacacacgaa
    84661 tgattttaca aaaaagatgc caaccttttt gtttgaatca cccgtgtgct gtttttatta
    84721 tcaatctatc tttacttata ttgttcctcc aaacggctca tccaccaacc tagtgctgta
    84781 ccggctataa aaagaacagg acaagctagt accccagcaa ctcccaatcc cgcgtaatcg
    84841 gtaggaatga tcataatcgt tgaagcaaaa actacaccta aaatgaaatg gaatagcttg
    84901 ctatatgcct ttgagaaaat gtaatccatg atttttgaca atccaagaac acaaagaatc
    84961 ccaccaattc cgatcggcac aatcacttca agtcgtactt ctttaaatcc gtcagccatc
    85021 gctttataca atcccatata caccaaaaaa ttagaagggc tcaatcctgg gacgatcata
    85081 cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa aattctgatc gacttgcccg
    85141 tcaaataatt gagcgccgta taataaaaac aaataagcca gaacaaaagt aacaaccatg
    85201 atgatcacat cagaagtatc tcttcctttt ttccctgctt ctttccataa cgcaggaaca
    85261 gttccagcga tacatccgac aaaaaaccat aatatcgttg tttcatacgc acctaataaa
    85321 aaactgacta aaaaagaaag tagaaagatg cctgtcactc cgcctagacc aacaggcaga
    85381 aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga aatcagccgc
    85441 tcatacatgc caaagacagc tgctaatgca ccaccgctga ctcctggcaa gataaatcct
    85501 gaaccgataa acattccctt cacaaaccgt aacatccagt ctttggttgt gtaccccact
    85561 tcttgatttt ccattttttg ccttttttcc actatttgcg cactccttct ttcatttaat
    85621 tttattcatg attgaacatt tcctgctatt ttacttaaag actttttcat aggtctctat
    85681 tattttttca ttcattttga caatatcagg ataaaacatg ttacctccat tatgatagag
    85741 atagccgcca ttgtaaagca aggactggat ccgccaatat cggtattgtt tcccttcctc
    85801 atttcctaaa agtgggctca gcacatcttt agaatacgct tctgctaatt ctactgtgtt
    85861 ttctccgcca tgatttttta cataggcaat ataatctttc ccaaaattat atgcttggac
    85921 agctgtccat aaatcgcatc cttgtttctt tgcttctact atcatttccg ccaaatattt
    85981 gacaccttga tcaatactgt cagctggatt atcaatgatc tcagcttctc catgcagact
    86041 ttccgagctc tgcatgagat ctgtcccttt tccttttgat tcggtcaata ttatcgcctt
    86101 gatcaaattc tcgtagtttt ccatatgata attttttgca gctgttgtta tttctatttg
    86161 atagctttcc actcgtttta ccgaacgata agttcttcct cctaaaaaca acaccgcacc
    86221 aataaccgtc agaatgatca tgtaaaaaag aatacggata atagataatc gttttcgctt
    86281 catttcatca cctagttatt ttga
//
LOCUS       DAPBBG010000004        78316 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPBBG010000004 DAPBBG010000000
VERSION     DAPBBG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 78316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.004"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            108..1172
                     /locus_tag="RD477_000293"
     CDS             108..1172
                     /locus_tag="RD477_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDT7632688.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1418..1843
                     /locus_tag="RD477_000294"
     CDS             1418..1843
                     /locus_tag="RD477_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7632689.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1836..2285
                     /gene="nusB"
                     /locus_tag="RD477_000295"
     CDS             1836..2285
                     /gene="nusB"
                     /locus_tag="RD477_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDT7632690.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2382..3236
                     /locus_tag="RD477_000296"
     CDS             2382..3236
                     /locus_tag="RD477_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDT7632691.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3237..4574
                     /locus_tag="RD477_000297"
     CDS             3237..4574
                     /locus_tag="RD477_000297"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDT7632692.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4579..4803
                     /locus_tag="RD477_000298"
     CDS             4579..4803
                     /locus_tag="RD477_000298"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDT7632693.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4805..5695
                     /locus_tag="RD477_000299"
     CDS             4805..5695
                     /locus_tag="RD477_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDT7632694.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5704..6522
                     /locus_tag="RD477_000300"
     CDS             5704..6522
                     /locus_tag="RD477_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDT7632695.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6629..7078
                     /locus_tag="RD477_000301"
     CDS             6629..7078
                     /locus_tag="RD477_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDT7632696.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7096..8778
                     /gene="recN"
                     /locus_tag="RD477_000302"
     CDS             7096..8778
                     /gene="recN"
                     /locus_tag="RD477_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDT7632697.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8833..8949)
                     /locus_tag="RD477_000303"
     CDS             complement(8833..8949)
                     /locus_tag="RD477_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDT7632698.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9022..9978)
                     /locus_tag="RD477_000304"
     CDS             complement(9022..9978)
                     /locus_tag="RD477_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7632699.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10149..10517
                     /locus_tag="RD477_000305"
     CDS             10149..10517
                     /locus_tag="RD477_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDT7632700.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10687..11118
                     /gene="mraZ"
                     /locus_tag="RD477_000306"
     CDS             10687..11118
                     /gene="mraZ"
                     /locus_tag="RD477_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDT7632701.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11139..12098
                     /gene="rsmH"
                     /locus_tag="RD477_000307"
     CDS             11139..12098
                     /gene="rsmH"
                     /locus_tag="RD477_000307"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDT7632702.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12103..12504
                     /gene="ftsL"
                     /locus_tag="RD477_000308"
     CDS             12103..12504
                     /gene="ftsL"
                     /locus_tag="RD477_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDT7632703.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12505..14697
                     /locus_tag="RD477_000309"
     CDS             12505..14697
                     /locus_tag="RD477_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDT7632704.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14723..15685
                     /locus_tag="RD477_000310"
     CDS             14723..15685
                     /locus_tag="RD477_000310"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDT7632705.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15699..17060
                     /locus_tag="RD477_000311"
     CDS             15699..17060
                     /locus_tag="RD477_000311"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDT7632706.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17086..18174
                     /gene="murG"
                     /locus_tag="RD477_000312"
     CDS             17086..18174
                     /gene="murG"
                     /locus_tag="RD477_000312"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDT7632707.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18189..19409
                     /locus_tag="RD477_000313"
     CDS             18189..19409
                     /locus_tag="RD477_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDT7632708.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19565..20890
                     /gene="ftsA"
                     /locus_tag="RD477_000314"
     CDS             19565..20890
                     /gene="ftsA"
                     /locus_tag="RD477_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDT7632709.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20913..22154
                     /gene="ftsZ"
                     /locus_tag="RD477_000315"
     CDS             20913..22154
                     /gene="ftsZ"
                     /locus_tag="RD477_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDT7632710.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22171..22848
                     /locus_tag="RD477_000316"
     CDS             22171..22848
                     /locus_tag="RD477_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDT7632711.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22862..23461
                     /locus_tag="RD477_000317"
     CDS             22862..23461
                     /locus_tag="RD477_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDT7632712.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23476..23763
                     /locus_tag="RD477_000318"
     CDS             23476..23763
                     /locus_tag="RD477_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDT7632713.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23812..24594
                     /locus_tag="RD477_000319"
     CDS             23812..24594
                     /locus_tag="RD477_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDT7632714.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24615..25322
                     /locus_tag="RD477_000320"
     CDS             24615..25322
                     /locus_tag="RD477_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDT7632715.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25584..28382
                     /gene="ileS"
                     /locus_tag="RD477_000321"
     CDS             25584..28382
                     /gene="ileS"
                     /locus_tag="RD477_000321"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDT7632716.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28431..29957)
                     /locus_tag="RD477_000322"
     CDS             complement(28431..29957)
                     /locus_tag="RD477_000322"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDT7632717.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29972..30619)
                     /locus_tag="RD477_000323"
     CDS             complement(29972..30619)
                     /locus_tag="RD477_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDT7632718.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30803..31132)
                     /locus_tag="RD477_000324"
     CDS             complement(30803..31132)
                     /locus_tag="RD477_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDT7632719.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31309..32037
                     /gene="sfsA"
                     /locus_tag="RD477_000325"
     CDS             31309..32037
                     /gene="sfsA"
                     /locus_tag="RD477_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDT7632720.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32053..33066
                     /locus_tag="RD477_000326"
     CDS             32053..33066
                     /locus_tag="RD477_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7632721.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33066..34343
                     /locus_tag="RD477_000327"
     CDS             33066..34343
                     /locus_tag="RD477_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7632722.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34406..37108
                     /locus_tag="RD477_000328"
     CDS             34406..37108
                     /locus_tag="RD477_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7632723.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37260..37577
                     /locus_tag="RD477_000329"
     CDS             37260..37577
                     /locus_tag="RD477_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7632724.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37607..37927
                     /locus_tag="RD477_000330"
     CDS             37607..37927
                     /locus_tag="RD477_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7632725.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38035..39495
                     /locus_tag="RD477_000331"
     CDS             38035..39495
                     /locus_tag="RD477_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7632726.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39563..39784
                     /locus_tag="RD477_000332"
     CDS             39563..39784
                     /locus_tag="RD477_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632727.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39815..39997
                     /locus_tag="RD477_000333"
     CDS             39815..39997
                     /locus_tag="RD477_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDT7632728.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39997..40410
                     /locus_tag="RD477_000334"
     CDS             39997..40410
                     /locus_tag="RD477_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDT7632729.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40533..41714
                     /locus_tag="RD477_000335"
     CDS             40533..41714
                     /locus_tag="RD477_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7632730.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41785..41949)
                     /locus_tag="RD477_000336"
     CDS             complement(41785..41949)
                     /locus_tag="RD477_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632731.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42103..42176
                     /locus_tag="RD477_000337"
     tRNA            42103..42176
                     /locus_tag="RD477_000337"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42137..42139,aa:Arg,seq:cct)
     gene            complement(42245..43384)
                     /locus_tag="RD477_000338"
     CDS             complement(42245..43384)
                     /locus_tag="RD477_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7632732.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(43683..44318)
                     /locus_tag="RD477_000339"
     CDS             complement(43683..44318)
                     /locus_tag="RD477_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632733.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(44431..45066)
                     /locus_tag="RD477_000340"
     CDS             complement(44431..45066)
                     /locus_tag="RD477_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDT7632734.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(45100..45561)
                     /locus_tag="RD477_000341"
     CDS             complement(45100..45561)
                     /locus_tag="RD477_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDT7632735.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(45691..46122)
                     /locus_tag="RD477_000342"
     CDS             complement(45691..46122)
                     /locus_tag="RD477_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDT7632736.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(46140..46460)
                     /locus_tag="RD477_000343"
     CDS             complement(46140..46460)
                     /locus_tag="RD477_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7632737.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            46759..47496
                     /locus_tag="RD477_000344"
     CDS             46759..47496
                     /locus_tag="RD477_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDT7632738.1"
                     /translation="MNTPQIFNFEQNEVRTFLENDIPYFVANDVAKTLGYKNPSDATN
                     KHCKKAVKTWGSDSLGRRQSFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLE"
     gene            47520..47723
                     /locus_tag="RD477_000345"
     CDS             47520..47723
                     /locus_tag="RD477_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7632739.1"
                     /translation="MTDIAEITQRDREKIKAYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            47739..48059
                     /locus_tag="RD477_000346"
     CDS             47739..48059
                     /locus_tag="RD477_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDT7632740.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            48064..48243
                     /locus_tag="RD477_000347"
     CDS             48064..48243
                     /locus_tag="RD477_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632741.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKASREGLTR"
     gene            complement(48363..48941)
                     /locus_tag="RD477_000348"
     CDS             complement(48363..48941)
                     /locus_tag="RD477_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632742.1"
                     /translation="MVVFSMNYILYVLLVVYAIIYIKQIVNAVSEITTINYLLTTIEK
                     VINDLKRLNDHSGSYKSDPYFQNNVTPIREIMVKEMPRINKVLPYSLVTLSLNQSDDI
                     LEENFRIIASQIFDKNNEIHYRKFWFLNPLIALKKFFLLPSEILSWFGINLSTFPGRI
                     FSLVIWVTSLFAQETISQILSFVITHFFKGQK"
     gene            48980..49678
                     /locus_tag="RD477_000349"
     CDS             48980..49678
                     /locus_tag="RD477_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDT7632743.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            49860..50195
                     /locus_tag="RD477_000350"
     CDS             49860..50195
                     /locus_tag="RD477_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632744.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            50428..51369
                     /locus_tag="RD477_000351"
     CDS             50428..51369
                     /locus_tag="RD477_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDT7632745.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSEATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            51371..52261
                     /locus_tag="RD477_000352"
     CDS             51371..52261
                     /locus_tag="RD477_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDT7632746.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            52304..53104
                     /locus_tag="RD477_000353"
     CDS             52304..53104
                     /locus_tag="RD477_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDT7632747.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            53119..53970
                     /locus_tag="RD477_000354"
     CDS             53119..53970
                     /locus_tag="RD477_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7632748.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            53967..54284
                     /locus_tag="RD477_000355"
     CDS             53967..54284
                     /locus_tag="RD477_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632749.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            54277..54459
                     /locus_tag="RD477_000356"
     CDS             54277..54459
                     /locus_tag="RD477_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632750.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            54467..54853
                     /locus_tag="RD477_000357"
     CDS             54467..54853
                     /locus_tag="RD477_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632751.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEVE"
     gene            54850..55185
                     /locus_tag="RD477_000358"
     CDS             54850..55185
                     /locus_tag="RD477_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632752.1"
                     /translation="MIILATDYIDSLKDEDGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSESAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            55185..55415
                     /locus_tag="RD477_000359"
     CDS             55185..55415
                     /locus_tag="RD477_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632753.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLSDGTEIVIRQSYLKDMAAP
                     VLIDRFNVTDSAVENHLKEFRSSIDDTFRLGS"
     gene            55422..55619
                     /locus_tag="RD477_000360"
     CDS             55422..55619
                     /locus_tag="RD477_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDT7632754.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            55616..55822
                     /locus_tag="RD477_000361"
     CDS             55616..55822
                     /locus_tag="RD477_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632755.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLSIVDILQSAKWFDNLEEAN
                     QVSRRLGGLTQVYELITIDHEEVE"
     gene            55822..56115
                     /locus_tag="RD477_000362"
     CDS             55822..56115
                     /locus_tag="RD477_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632756.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDKWLAKSKENLAVVRLVDMEVIKS"
     gene            56145..56585
                     /locus_tag="RD477_000363"
     CDS             56145..56585
                     /locus_tag="RD477_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7632757.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            56660..57127
                     /locus_tag="RD477_000364"
     CDS             56660..57127
                     /locus_tag="RD477_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDT7632758.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            57422..57559
                     /locus_tag="RD477_000365"
     CDS             57422..57559
                     /locus_tag="RD477_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632759.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQLQEALDEIANFKIQV
                     S"
     gene            complement(57540..57854)
                     /locus_tag="RD477_000366"
     CDS             complement(57540..57854)
                     /locus_tag="RD477_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632760.1"
                     /translation="MSNGFDDLSKKLNKMAKAAQEMDGEHSVPMSELFTNDFVSANTK
                     FSSIEEFFEVSGFDCSSEESFAAIPDDQMDAFVSDNTNFDTWSDFMSAAAIKYTAKKL
                     GF"
     gene            58846..59190
                     /locus_tag="RD477_000367"
     CDS             58846..59190
                     /locus_tag="RD477_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDT7632761.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            59195..59476
                     /locus_tag="RD477_000368"
     CDS             59195..59476
                     /locus_tag="RD477_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632762.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            59578..59892
                     /locus_tag="RD477_000369"
     CDS             59578..59892
                     /locus_tag="RD477_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDT7632763.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            59870..61564
                     /locus_tag="RD477_000370"
     CDS             59870..61564
                     /locus_tag="RD477_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDT7632764.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            61584..62762
                     /locus_tag="RD477_000371"
     CDS             61584..62762
                     /locus_tag="RD477_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7632765.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKDFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            62725..63411
                     /locus_tag="RD477_000372"
     CDS             62725..63411
                     /locus_tag="RD477_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDT7632766.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            63411..64571
                     /locus_tag="RD477_000373"
     CDS             63411..64571
                     /locus_tag="RD477_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDT7632767.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            64581..65456
                     /locus_tag="RD477_000374"
     CDS             64581..65456
                     /locus_tag="RD477_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632768.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            65453..65764
                     /locus_tag="RD477_000375"
     CDS             65453..65764
                     /locus_tag="RD477_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632769.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            65754..66107
                     /locus_tag="RD477_000376"
     CDS             65754..66107
                     /locus_tag="RD477_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDT7632770.1"
                     /translation="MANNRRLEETFNDGWLKILTQSTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            66097..66498
                     /locus_tag="RD477_000377"
     CDS             66097..66498
                     /locus_tag="RD477_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632771.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            66491..66895
                     /locus_tag="RD477_000378"
     CDS             66491..66895
                     /locus_tag="RD477_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632772.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            66907..67515
                     /locus_tag="RD477_000379"
     CDS             66907..67515
                     /locus_tag="RD477_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7632773.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            67535..67897
                     /locus_tag="RD477_000380"
     CDS             67535..67897
                     /locus_tag="RD477_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632774.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            67900..68082
                     /locus_tag="RD477_000381"
     CDS             67900..68082
                     /locus_tag="RD477_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632775.1"
                     /translation="MPNLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            68099..71419
                     /locus_tag="RD477_000382"
     CDS             68099..71419
                     /locus_tag="RD477_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7632776.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALDMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEKFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFKGIWSKF
                     TDWLGGTWESIKEGASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPHFKLSGKFNPLKGKIPTLGVDWYAKGSVFNSPNIIGIGEAG
                     PEVALPLKRSVLQEIGDRILSSTSVSSRAQTVQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            71470..72207
                     /locus_tag="RD477_000383"
     CDS             71470..72207
                     /locus_tag="RD477_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7632777.1"
                     /translation="MTDCIHSIVDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            72217..74508
                     /locus_tag="RD477_000384"
     CDS             72217..74508
                     /locus_tag="RD477_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7632778.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWILVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            74532..76730
                     /locus_tag="RD477_000385"
     CDS             74532..76730
                     /locus_tag="RD477_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632779.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEENSKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVITLDPNHKLEVLKPKSQPALLTGKTYTVSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPFQPNLLAEPYNMCREYPNENIADPTVKFPIESGDH
                     QIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTF
                     TPTNVVAMPKLFRVYQYPRSTVGACRIDWLKIEKGNTRTPNISEYKYRGTGMRDSNNP
                     KDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSKINSQLTASINEVDTTAKGA
                     QTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVG
                     ETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVSYGVD
                     ALQNRFAASGNIVTDLEVNDVLNMRTEIGVANGILQFTSCRTLVLTRLSD"
     gene            76808..77050
                     /locus_tag="RD477_000386"
     CDS             76808..77050
                     /locus_tag="RD477_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDT7632780.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            77066..77263
                     /locus_tag="RD477_000387"
     CDS             77066..77263
                     /locus_tag="RD477_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDT7632781.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            77274..78293
                     /locus_tag="RD477_000388"
     CDS             77274..78293
                     /locus_tag="RD477_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDT7632782.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGLNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT        26769 a        12758 c        16386 g        22403 t
ORIGIN      
        1 tatttaaaat aaaaagaagt tttccggaaa tttttattta actgtcgtgt acgaagaaaa
       61 gtttgctata atggaaaaaa gcgtgcaaag aaagagggag tttcattatg atgctacgtg
      121 tagaaaaatt gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg gttaccagtc
      181 cgtacaatct tcgttactta acgaacttta caggtacgac aggattagct gttattactt
      241 tggaaaaagc attttttatt actgattttc gttataccga gcaggcggca gcgcaggctc
      301 aagggtttga aattattaaa aatgtcggac ctatttttga tgaggtagca aacttagtac
      361 gcaaagaaga actgcactca ttagcttttg aggaaacaac agtctcattt ttagaatatt
      421 cggttttaga agaaatcatc gatgcggaat tagtacctgt ttcacagatg atcgaagaac
      481 tcagagaagt gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt catattgcgg
      541 atatggcata tgatcacatc ctgaaaatga tccaaccggg catgactgag attgaagtcg
      601 caaatcaact agacttttat atgcgttctc ttggcgcttc aggtgtttct tttgatacga
      661 tcgttgctag cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg
      721 aacaaggtga cttgatcaca ttggactttg gttgctatta cgaagggtac gtttccgata
      781 tgacacgtac attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag
      841 tcttagatgc tcaattagca gttattgatg ctgcaaaacc aggaatgact ggtgtccaat
      901 tagatgcagt tgctagagat tatatttcaa aacatggtta cggggaagca tttggacatt
      961 ctacaggaca tgggatcggt ttagagatcc acgaaggacc aaacgtttct tctcgtgcag
     1021 aaaagcaatt tgtaccagga aatgtgatca ctgatgaacc agggatctac ttaccaggaa
     1081 tcggtggagt acgtattgag gatgacttgt tgatcacaga agatggaaat cgtgtgttga
     1141 ctcgttcacc aaaagaattg atcatcttat agtttcatca atagaagaag taagcgtaaa
     1201 aatcagtgtt cacagacttt gttatagaaa atcactcgat ttaatgatac actatagaag
     1261 aacttttaag ctgtaagaga taataaagtt gggtgttttc tccaattcat agcagtttgc
     1321 agctgatcgg cggcagtttt cttaccacgt gatcactatg ccgcatcata gagaattcgg
     1381 atgtatctcg tgcttaggca aagggaggaa taaataaatg gcagatgaaa aaaatttgat
     1441 tttaaacgca aaccaagatt taggagagat cgtgatcgct cctgaagtca tcgaagtaat
     1501 tatcgggatt gcagcttcta aagtagaagg cgtatacgga atgcgcggaa cttttgccag
     1561 caatgtcact gaattattag gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga
     1621 ggaagatggc ttgaaagtag atctttactg ttatctagaa tatggtgttt cagttcctaa
     1681 agtagcaatg gaaatgcagg aacgggtcag ccagcaagtc cttttcatga cggatatcga
     1741 tttatctgaa gtcaatatcc atgtagtagc tgttgttcct gaaaaattag aacaaccaac
     1801 aatcgaagaa ctttttgaag acgaagagga agagaatgaa taaagaatta tcaagacacg
     1861 agattcggga aatggcattg caggctttat ttccattaga cttcaatgca gacctaacaa
     1921 aagaagatgc gattttcaac gcaatcgaat tagaccaccg cgacatgatc aacgaagatg
     1981 aatcagagtt tgttcctgtt tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag
     2041 atgaattgga taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc aatcggatct
     2101 caaaaatgga tttggtgatc ttgcgtatcg cgatttttga aatgacttat gtgacagatg
     2161 taccagcggc tgttgcttta aatgaagcaa tcgagttggc aaaaacattt agtgatgacc
     2221 gttcacgtaa attcgtcaac ggtgttttat ccaatgtatt gaaagaatta gaagctgggg
     2281 cgtaagtcct agcttttttc tatgaatcat cataaacctg ttataaatcg ggaaaattct
     2341 atggtagaat gaaaacacaa aaaatatctg ggagtggtta gatggcagaa ttgattaacg
     2401 gaaaagaatt agctgaaaaa atgcaggcag agattgctga aaaaatccaa aaattgaaag
     2461 ataatggtat ccatcccgga ttagtcgttt tattggtagg ggaaaatcca gccagccaaa
     2521 tctatgtaag aaataaagaa cgtgtagcaa aagaaatcgg aatccattca ttggtagagc
     2581 gttatcctga gacgatttct gaagacgaac tacttgcaga aattgaaaaa tacaatcaag
     2641 atcctagttt tcatggtata ttggtccaat taccattgcc aaagcatata gatgaagaaa
     2701 agatcctgtt agctatccgc cctgaaaaag acgtagacgg ttttcatccg atgaatttag
     2761 gcagactcct tgcgggtaaa ccggacaaga ttccttgtac tccatacggc atcatgaaaa
     2821 tgtttgaagc ttattctatc gatccggcgg gtaaacgagc agtagttatc ggacgaagca
     2881 atatcgtcgg taaaccgatg gctcagctac tcttgatggc agatgcaact gttactgttg
     2941 ctcattctaa aaccgaaaat ctggctgaac tggcacaaga agcagatatt ttagtcgtag
     3001 caataggacg tggacatttt gtcacaaaag aattcgtaaa accaggtgct gtagtgatag
     3061 atgtagggat gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa tttgatgaag
     3121 tggagcctat tgcaggctgg atcactcctg tgccaaaggg tgtagggcca atgactatca
     3181 cgatgctgat gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg
     3241 cacaagaata tttaactgtc actacattaa cgaagtattt gaaaagaaaa tttgatgccg
     3301 atccttattt agaacgcgta tatttaacag gcgaaatatc gaatttccgt ttacgtgcca
     3361 atgcccatca atatttcagc ttaaaagatg accatgcaaa aatatctgct attatgttca
     3421 aaggagcatt ccaaaaatta cgattccagc caaaagaagg aatgaaagtc cttgtcgttg
     3481 ggcgtatttc attatatgaa gcgggaggtt cttatcaaat ctatgtagag cacatggagc
     3541 ctgatggaat aggtgcttta tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg
     3601 aaggcctttt tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc
     3661 ttaccagtcc aagcggagcg gttatccgcg atatcatcac gacggtgaaa agaagatatc
     3721 caattgtcca attggttttg ttcccaacag ttgtacaagg aaaccaagca gcggacaatg
     3781 ttgtcaggaa tatccagaga gtagaagaga tgggcaattt cgatacaatg atcatcggtc
     3841 gtgggggtgg ttcgattgag gatttatggc cgtttaatga agagcgggtt gctcgggcga
     3901 tttatcaagc taatacgccg gtgatttcat ctgtaggaca tgaaacagat accacgatcg
     3961 ctgatttagt agcagatgtt cgggctgcca ctccaacagc agctgcagaa ttagctgttc
     4021 ctgttttgtc agaagagttg atgcgcattc aagaacggca agctagattg cagcaggcgt
     4081 ttgtacgcca gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat
     4141 ttcggcagcc tgaaagattg tatgaaggtc agaccatcaa attagaccag ttgaaacagc
     4201 gattatatca agctacacag caaatttatc atgcgaaaga aaaacaaacg atcgcattaa
     4261 gccaccaatt ggaacaagtg gctccgcttt atcgagttaa agcagctcgc caagaaactg
     4321 cttatctaga aaaacgctta gaagagaaaa tccaacaata tatgaaacaa cagcagcagc
     4381 gttttgctca agctgttcaa tctcttgatt tgttgagtcc attgaaaatc atgggaagag
     4441 gctacagcta tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag attcatcaag
     4501 gggatttgct gacgatccat tatcaagatg gaatcgttaa aggacaagta caagagatag
     4561 acaaaggaga ataaaaatat ggaaaatcca acttttgaag agtcactgca agaattagaa
     4621 aaaattgtca tgcagttaga acaaggagac gttccattag aatcagctct agattcgttt
     4681 aaacgaggaa tggaattaag caaacattgc caagatacat tagctaaagc agaagagacc
     4741 ttgacgaaaa tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc
     4801 tgagatgaga ttaactgact tttcagctat tcatctacct gctgtagaaa aagaaatctt
     4861 gtctttttta gatgagcata caacggatag aggactctat gatgcgatga gttattcagt
     4921 gaaagcaggc gggaaaagaa tacgtccctt attactttta acggccgttg cttcctttga
     4981 tgagcctatt gatgttcccg tctatcaagt agcagctgct ttagaaatgg tgcatacgta
     5041 ttccttgatc cacgatgatt tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc
     5101 taccaatcat aaagtattcg gcgaagcatt agctatattg gccggagatg gtttgcttac
     5161 aggagcattc cagctaatca gtatggcaca cttaggtaat tctcctaagc ttctgctgct
     5221 tcagcaatta gctgtttgtg cgggatcaca aggtatggtt gctgggcaag ctgcagatat
     5281 cgagggcgaa tcgaaaaagc tttctttaga agaattagca tttattcatg aaagaaagac
     5341 agggcatttg attcgttatg ccttgctggc tggtggcata ttagcgaagc aacctgaaga
     5401 aatcttattg ctacttcaac gattagccga acatttaggt ttagcttttc agataagaga
     5461 tgatctactc gatgtgattg gtacaacgaa aacattaggg aaaacagctg gtaaagatga
     5521 acgtatggaa aaaaatactt acccgcgttt attaggccta gaaaagacac gtgaggcgtt
     5581 ggagattgag ctgttatccg ccaacaagat catagacaaa ttagaagagg acgttttttc
     5641 ttttgatggc gggctgatgc gccagatgat caagcaattt gatgtggaga aatgagggaa
     5701 agaatggaaa aagaacgcgt agatgtatta gcggtcaaac aaggattatt tgaaacaaga
     5761 gaacaagcca aacgttcagt gatggctggt ttgatttaca acgaaaaaaa cgagcggttt
     5821 gataaaccgg gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg taaaaaactt
     5881 ccttatgttt ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt tgatctatct
     5941 gttgaaggaa aaacattgtt agatattggt gcatccactg gcggatttac tgatgcagct
     6001 ttgcaaaatg gcgcaaaaat gagttatgct ttagacgttg gctacaatca gctagcttgg
     6061 aaaataagac aagatcctag agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa
     6121 cccgaagact ttaccttagg aattccagaa gttgctgtta tcgatgtttc ttttatttca
     6181 ctaaaactga tgctgcctcc attacatgct attttaaaag aagagggaga agtgattgct
     6241 ttaatcaaac cccaattcga agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt
     6301 gaccctcaga cccatcaaaa agttttagaa gacattttgt catttgcggc acaaggtgga
     6361 tatgatgtgc tggaattaag ttattctccg atcacggggg gagaaggaaa tatcgagttc
     6421 ttggctcact tgaggaaagt acccgaaagc ggaacaatca attcagctat taatatggca
     6481 gaagtggttt caaatgcaca cgaacagttt gaccataaat aaattgtaac tgtgaaaatc
     6541 aagtgagaag tatgcggatt cttctttatt ataccaagga aataagctat cattgattaa
     6601 gaagaatatg ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc gtttgattac
     6661 tcgattgttg agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag
     6721 caaaggaata tctgttactc aagctacgat ttccagagat ataaaggaac tgaaactgat
     6781 caaggtaccg gcttcagatg gcggctaccg ttatagcata ccagctgaaa caaacgaaga
     6841 tgtaaatact aaattggaaa aactattaaa agacgctttc gtggcagttg accaaatgga
     6901 aaaatttgtt attttgaaaa cattgccggg aaatgcttcg gcagcagcaa atttgattga
     6961 taaacgttac aagaaagaat tattttcgat tatcaatgat gacgataatg ttttgatgat
     7021 tacacgaact gaagaccagg cagttttatt gaagaaagac ttcctgcatt acctttaagt
     7081 tgaggagcga atgtgatgct acaagaaatc agtattacta actttgcgat tattccggaa
     7141 ttgcggcttt cttttcacga aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa
     7201 tccattatta tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag cgattatata
     7261 cgtcaaggag cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa acaagaagga
     7321 ttctctgaac tgatggttga attagggatc gaaacagatg aggacaatct gattgttcga
     7381 cgggatatgt ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat tatcacatta
     7441 gctaatctga gaagaatcgg cagttattta gtagatatcc aaggacaaaa cgaacatcag
     7501 gaactgctcc agcctgaatc acatcttgct ttattggatc gttttggaga tgtagctttc
     7561 caacagaaga aaaagagtta tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg
     7621 gtacgaaaag tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat gctccatttc
     7681 cagcaagaag aaatagctgc tgctgatttg caagtcggag aagaagagaa attaaaagaa
     7741 gaaagagaaa aacttagcaa ttatcaaaaa attgctgatg gattagctgc aggttacgga
     7801 gcactgacga ataatgaaca aaacagtgta gatggtgttg gcttggccgt ttcagaactg
     7861 caagggatcg cccatctaga tgtggagtat gaagcaattt atgaaaatat ccaaagtgct
     7921 tactatttgc tgcaagatgc aatcggcgat atgagtcgtc agattgattt attggagtta
     7981 gatgagagtc gtttagaaga agttacgcaa cgtttagaat taatccgaca gttgaaacgt
     8041 aaatacggcg aatccattga gtcaatattg gcttactatg acgaaatcac cgaagagcta
     8101 gcttcttctg atttttcaga aggtcaattg gacaaaatga aagaggaact agagcaaaaa
     8161 gagcttctac ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa
     8221 gaattggaaa aatccattct gcatgaattg aaaagtctgt atatggaaaa cactgagttt
     8281 gaggtacgtt tcttaaaaga agaaaataga caattgaatc atgatggatt tgaccaaatc
     8341 gaattttaca ttacgaccaa tccaggagag ccactgaagc ctttggtgaa agtcgcttct
     8401 ggtggcgaac tgtctcggat gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt
     8461 gtgacaagta ttatttttga tgaagtggat acaggtgtga gcggaagagt tgcacaggcc
     8521 atcgcagata aaatttctca aatatcgaaa tactctcaag ttttgtgcat cactcatctg
     8581 cctcaagttg ctgcagtcgc cgactatcaa tattatattg tgaaagcagt gattgatgga
     8641 cgtacgcaaa cttctgtttc agagttgaaa acaaaagaaa gagaagaaga aattgcacgt
     8701 atgctggctg gaagtgagat tacaaaactc acactcgaac atgcaaaaga actattgaaa
     8761 ctcgcaaaaa ggacctgagc cacctcgcag tggttcaggt ccttttcagc agctgatagc
     8821 tatattttat ttttacatga cactcattac agcagtcaat acaacagatc caatcgttaa
     8881 aatcagcatt gtccaaatga caaattttgt tactttagca aaagtacttg tttgtttttt
     8941 ttcacgcatt gttttcaacc tcgtcattaa ttagttgatt caagtgtaca atcttttttc
     9001 catttccaca agagggcttt tttataaaaa cttccctttt tttaaataat aggtagttac
     9061 gatacaaata aataatgtta atagaactag ccatataaag gcatcatccc tttcagcaat
     9121 aggtaactta atgttcattc catagatact gccgacgatt gtcggtatag ttaaaataat
     9181 cgttaaagaa gttaaaattt tcataatatt attcagatta ttcgatacga ttgctgaaaa
     9241 agtctgattc atttgttcta ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc
     9301 tgtttccacc aatacatcac gtaaatgatt tttcaaagca tgattttctt ggaaaagttt
     9361 attttgcgct agttctttca atgcatctaa attagcagaa gtcgcttctc tgaaataaac
     9421 gaggcttttt tggatatcca tgatttgata aagttgtttg ttttcagtgg atacttgaag
     9481 tttggcttcc aaagtatcgc attgagattg aagcgtagct aaataactgt tgtaagagag
     9541 tgtgacctgc caaatgaatt gaaagaagat attgagatta ggcgaaatat cactttttgg
     9601 aaaggtctgg ctaaagactt tctttaaaaa cagtggttca tgattcgtaa ttgtgataag
     9661 cttctttttg ggcgtcaaaa taatagagag cgggtatgta tccacttgaa aataaccgct
     9721 aggacttgtt ttagcaaaag gatattgtaa caataataac gctggctttg tcaactttga
     9781 ttgattgaat tcttctgtac gggaattttc tgcatcgtcc aatatggaag tgatgtaatc
     9841 tgacggaatt tcaaattgat gtgtcagctg atgaatctct tcttgattag gacgttctac
     9901 acaataccag atcgtttggt cagtttcctt tccggtatta cgcagtttct cattatctaa
     9961 tgtaaaatat ttgatcatga ttttcctcct gttgcctgtt tcttttatta taacggatag
    10021 ggaattggaa tacatggaat gtgtttgtaa gatatagaat ataactgcat aaatttaaat
    10081 aaaatgaaac agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt
    10141 ttacgttcat gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg
    10201 ttttgtttgg ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa
    10261 aagcccctga tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata
    10321 cagggacaat ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta
    10381 ttgctgtttt ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga
    10441 cttggcgttt ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca
    10501 tactttctta cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg
    10561 cagcttgatt tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa
    10621 aaggtggtag aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg
    10681 ctttcaatgt tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg
    10741 ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc
    10801 tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg
    10861 ccgttggcta aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa
    10921 tgcgaacttg ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct
    10981 ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca
    11041 cgatggcaag atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg
    11101 attgatttcg gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact
    11161 atacagtcat gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg
    11221 tagactgtac actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta
    11281 aagggcatct gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt
    11341 tagcttctta tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag
    11401 aagaacgctt gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt
    11461 cctctcctca gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg
    11521 atatgcgcat ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct
    11581 accatgaatt agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg
    11641 ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg
    11701 aaatcattaa acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa
    11761 gaatttttca agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt
    11821 tagaacaggc aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt
    11881 cattggaaga cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac
    11941 caccgggact tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta
    12001 aaccaatcct tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt
    12061 tacgtattgc agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa
    12121 atgcaagatt acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct
    12181 gattcttctt cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag
    12241 atttctgctt tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt
    12301 gttttaacag ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata
    12361 caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa
    12421 ctttctcgta ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac
    12481 gacaatttaa ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa
    12541 aagaaaaatc taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg
    12601 agtattggat tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat
    12661 gttgctggta cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta
    12721 aaagcaaaaa gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc
    12781 tcttattcaa ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat
    12841 gttgaagaaa agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac
    12901 aaaaaagatg cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa
    12961 tttggtagct atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg
    13021 aaaaaagaag gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat
    13081 ggcgtctttt cttctcattt tattggatac gcagttccag acaaagatga gaatggctta
    13141 gttggtaaac taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag
    13201 attatttatc aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa
    13261 aaagctgtcg acggacaaga tatctacacg actttagaca gcagactaca aagttattta
    13321 gaaacactga tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg
    13381 atgaaagcaa aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa
    13441 acaatggaag gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt
    13501 tatgaaccag gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa
    13561 ttcaacgaga atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat
    13621 gaccatgact ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt
    13681 aacgtcggga tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa
    13741 aaactgggat ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta
    13801 cctacattaa atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg
    13861 aatttccaaa tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct
    13921 cgatatatca gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa
    13981 gttctagggc aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat
    14041 gttgtcgaaa gcgaaaacta cggtattgct tatggtgtat atagtgtacc tggatacaat
    14101 gtatcggcaa aaaccggaac agcccaaatc gcttctgata cagggggata tcaaacagga
    14161 gatacagcct acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc
    14221 ttgtatctaa caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca
    14281 aatccattga tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa
    14341 acaaaaacag aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca
    14401 gctgatgctc aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa
    14461 aaacaatcta ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact
    14521 ggtggtgata agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg
    14581 gggaaaatcc tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa
    14641 cttgctccat atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa
    14701 gagaaatagg agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag
    14761 ttgtgcaatg acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca
    14821 aggtcaggcg attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac
    14881 aatgggcggc ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg
    14941 gcaaaaacag ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt
    15001 aatcggcttt cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc
    15061 gaagcagaaa ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc
    15121 tgaaggttat ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta
    15181 tggtattttc gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg
    15241 catcgatgga ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc
    15301 ttggcatcag catcagtatg atgtattaat catttgctta agtgttttag gaggtttact
    15361 gggtttcttc gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt
    15421 agcgttgggc ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct
    15481 tttgattggt ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt
    15541 taagcttaca ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg
    15601 cggatggtcg gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt
    15661 gatcacatta tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg
    15721 aaaataagaa agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat
    15781 tacatgaatt gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg
    15841 aagcacaaga gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat
    15901 tgttggatga agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt
    15961 tagtagcaaa agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg
    16021 aagtagcgga atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa
    16081 cgatgacagg actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta
    16141 atattgggta tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga
    16201 tggaattgtc tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga
    16261 ttaccaatat ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag
    16321 ctaaatggaa ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc
    16381 agtcagaact acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa
    16441 aagaagtgtt ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa
    16501 aaataatgga aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag
    16561 ctgctatttc tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc
    16621 attttttcca tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat
    16681 tttataatga ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg
    16741 aaacgtctaa ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat
    16801 taattccttc attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt
    16861 tgatggatgc tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac
    16921 aagcagttcc tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag
    16981 cgaatgccag ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag
    17041 ctgtgaaccg attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt
    17101 acaggcggag gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag
    17161 actcaagaac cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa
    17221 atcgtccctg atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa
    17281 atttccatgc ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa
    17341 aaaatcctca gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga
    17401 gcggtagtgt acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt
    17461 gtaccaggtg ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt
    17521 aaagatgcgg cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca
    17581 caagaagtag cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa
    17641 aagaaaacag tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta
    17701 tcagtttttt taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat
    17761 agatattatg aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag
    17821 ccttatatca aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct
    17881 ggtgcaactt cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc
    17941 ccatatgtta cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca
    18001 gcaaaaatga ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa
    18061 atcatgggtg atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc
    18121 cctgatgctt caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg
    18181 tggtcttagt gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa
    18241 acaatttgac tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa
    18301 accaagagcc aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag
    18361 aaaaaacggt tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt
    18421 ttgaagagat caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc
    18481 tgcccaattt aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca
    18541 ctgtattggg tataccgctg atttttttga tctattatgt ttctccttat agcaaattac
    18601 aagcagttgc agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa
    18661 aattgtcatt aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc
    18721 gccttaaaaa agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg
    18781 aattcgattt ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat
    18841 actacccagt gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa
    18901 atctccctat cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat
    18961 acaatcagtt atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag
    19021 caagcaataa aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata
    19081 tcaataatct tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa
    19141 aaggtgtgat cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa
    19201 cggaagaatc gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa
    19261 acgaatcaag tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa
    19321 acggatcatc tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta
    19381 caactccgtc aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt
    19441 tctaaaaacc agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat
    19501 attttaaaac ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc
    19561 attcatggca aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt
    19621 tgtcgtggct gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc
    19681 agaagggatt aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg
    19741 agcagtacga caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt
    19801 gccagcaaac atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc
    19861 aaaggaaatc accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat
    19921 acctcctgaa cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga
    19981 gggaatcaaa gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt
    20041 tacaggacct aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat
    20101 cgtcaacgaa atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga
    20161 aaaagatttt ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat
    20221 gcatgataag caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa
    20281 agatatctct attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta
    20341 cggggatgca tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg
    20401 tcagtcagaa ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat
    20461 ggaacagatt ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc
    20521 aggtggcgtc gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca
    20581 agaaatcttc ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc
    20641 ggtatttaca aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca
    20701 attggcaaaa attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca
    20761 agaaacaaca aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc
    20821 ggaaccaaaa gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat
    20881 ttttgattaa ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca
    20941 atgacggcgc agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc
    21001 gaatgattga agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag
    21061 cattaaaaaa ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt
    21121 taggtgctgg ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct
    21181 tacgcgaagc tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa
    21241 ccggtacagg tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg
    21301 ttggtgtcgt gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag
    21361 aaggtatcgc tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt
    21421 tattagaagt tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg
    21481 ttttacgaca aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc
    21541 ttgactttgc tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg
    21601 gtgtggctag tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat
    21661 tgcttgaaac atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg
    21721 atatgacatt gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg
    21781 atgtaaatat cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga
    21841 cggtcatcgc aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta
    21901 gacaagcaca aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac
    21961 caacttcttc agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg
    22021 ttcgtcctag agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat
    22081 tcaaccgcga agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc
    22141 gtcgtaaaaa ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag
    22201 caagaaattc aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta
    22261 gctgtaacaa aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg
    22321 aattgtgcag aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt
    22381 cctgaaataa agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta
    22441 aatgaaattg attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag
    22501 cggttagaga aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa
    22561 catggaattt caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa
    22621 atcaaggttg ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat
    22681 aaagtgtttg gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat
    22741 gcaccatgta gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa
    22801 ggtgcaacat ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa
    22861 aatgccgctt ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga
    22921 gtacgaatac caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa
    22981 tacaacacag aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa
    23041 aacggcgact tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt
    23101 aattgaacta catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac
    23161 ctctacacaa cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta
    23221 ttctgaagct atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt
    23281 tcatttagta gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta
    23341 tgcactagat ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg
    23401 gatggagatt gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta
    23461 agaggaggaa ctacgattta tactattgca ctattacttc atttacttca acgggcagcc
    23521 tatttctata gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat
    23581 caatcagggc taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat
    23641 cgtctgcctt tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg
    23701 aacttagccg tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat
    23761 tgattgaagc aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca
    23821 aatgtatacc agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg
    23881 attgaacagg ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa
    23941 tcctatattt tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc
    24001 ggatatgaac aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta
    24061 gaggaagatt ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta
    24121 agtcatggga aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc
    24181 gatatcatct cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt
    24241 gttgctgcac aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac
    24301 acagaaatga ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg
    24361 cgactagaca gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg
    24421 atcgaagctg gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt
    24481 gaactattag atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag
    24541 gggaagacga aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa
    24601 aaggtggttt actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa
    24661 aaatgcgggg atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact
    24721 atgaggaatt gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa
    24781 aacttgagta cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag
    24841 atacggctga taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg
    24901 cacaaaacaa agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa
    24961 ctgatgcaga agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc
    25021 ttgcgacaga aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct
    25081 tgatgcttga gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac
    25141 cattctccag ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc
    25201 ttgacaatga aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga
    25261 cggcatttga tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt
    25321 agaacagaaa agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag
    25381 acactgcatg gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag
    25441 cagcggctga cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt
    25501 gggtggtacc acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa
    25561 caaataaagg agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa
    25621 aattccctat gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag
    25681 aaaatcgaat ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc
    25741 atgatggacc tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca
    25801 gtaaagacat tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag
    25861 gatgggatac acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta
    25921 aagaaatgtc catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag
    25981 ataagcaacg agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg
    26041 ttacattaga tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa
    26101 aaagctatat ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt
    26161 tagcggaagc tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca
    26221 atgtcaaaga cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa
    26281 caccatggac attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat
    26341 tggtggaagc cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat
    26401 cagaaatcgg ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat
    26461 atatgactgc aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg
    26521 ttactttaga cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact
    26581 atatcgctgg tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt
    26641 ttacagaaga agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca
    26701 ctgatctatt aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc
    26761 ctcacgattg gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat
    26821 ctatcgataa attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc
    26881 catggggtaa aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc
    26941 gtcagcgtgc atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca
    27001 tcacaccaga aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct
    27061 ggtttgaaaa agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta
    27121 atggcgaatt tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc
    27181 atgaagctgt tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag
    27241 gatcagacca atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg
    27301 gtgtagcacc atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta
    27361 agatgagtaa atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg
    27421 cagatatcct tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta
    27481 tggatatctt aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct
    27541 tattagcgaa tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac
    27601 gttctgtaga caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag
    27661 aagggtacga caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg
    27721 ttgatctatc gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag
    27781 atgattatca acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa
    27841 aattattgac tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag
    27901 aagaggagta cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag
    27961 aattgatgga tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg
    28021 aagaagcaag aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc
    28081 caaaacaaca agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga
    28141 tcgtttcacc agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta
    28201 tggtatttga tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct
    28261 gtcgccaagt tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt
    28321 gtgcaaaaat cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt
    28381 aaaagggaaa agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt
    28441 aattttccac gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt
    28501 tcaagtagtt cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc
    28561 ggttgtgttt ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact
    28621 tcttcccaat gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg
    28681 taagcttctg ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt
    28741 tccgtttcaa atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt
    28801 ggctgaatat agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg
    28861 acagatgttt tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct
    28921 ttttcagtca aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta
    28981 tcgatagtga atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct
    29041 tcttcgcgat agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga
    29101 acgaagtttt ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc
    29161 ttttctgtac ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct
    29221 acaacctgta aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag
    29281 cccccgcgat cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag
    29341 cgattgttcc attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg
    29401 atcatttctt ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct
    29461 ctgatttgct ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg
    29521 atcaaacgat tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc
    29581 gttccaaaaa attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag
    29641 tgtccatcta atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca
    29701 tcatgggatt gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc
    29761 aatgcacgga ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga
    29821 gcggttccga ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa
    29881 gacgggtaca atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca
    29941 tttttttcat tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg
    30001 ccgccttaaa gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat
    30061 aagctgcgat gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt
    30121 aatccaacaa ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat
    30181 aggactccaa tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc
    30241 ctccatgagg acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt
    30301 ccgaagtcac atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta
    30361 aatgttcaac taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt
    30421 gcagtccgct tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt
    30481 tcacaagttt agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga
    30541 tttgtttatt gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat
    30601 cttctcgatt tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag
    30661 tgtaacaaaa aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac
    30721 gtttttacaa caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat
    30781 atatatatac attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag
    30841 attcgtcata aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac
    30901 gtacgactaa acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga
    30961 tcaaaaacgt gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga
    31021 accatgtacc ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca
    31081 agtaaataaa acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta
    31141 tcaccaaatc actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt
    31201 cattgtaccg aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt
    31261 ttctacaaat tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag
    31321 caagtggaaa tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa
    31381 aaagacggag aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg
    31441 ataccaggcg ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat
    31501 gatctgattg ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat
    31561 cggctggcaa ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca
    31621 tttacggtat acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag
    31681 acggtggtcg gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg
    31741 attggtgctt ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg
    31801 tatgccgcac aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt
    31861 catctggcaa cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg
    31921 cagcaaagcg gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact
    31981 gtcaagcagg ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa
    32041 gaaggtaaaa taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt
    32101 gtccaagcag ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg
    32161 gaaaaagcac aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg
    32221 aagacttttt tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt
    32281 cactttagtc aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca
    32341 gctttttcga cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg
    32401 tatttttttg aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac
    32461 ttaccattaa aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc
    32521 tatgaccaag tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt
    32581 gcattgatgg atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca
    32641 aaagaagtcc attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg
    32701 atcttacgtt atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat
    32761 ttaccgtcag agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt
    32821 gaacaagcac aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct
    32881 gcagaaaatc caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa
    32941 gatccagctt ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa
    33001 gtgatttatg atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat
    33061 ttttaatgaa cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata
    33121 aagggtttat ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac
    33181 aaagtatagt agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc
    33241 tactactaaa cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag
    33301 aactagctat tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag
    33361 tccaatcttt ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac
    33421 cagaaatctt gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa
    33481 aagcacatca agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga
    33541 acagccaaat cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt
    33601 tttcagcgac agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg
    33661 agacaattga cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac
    33721 gtgtaccgat acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc
    33781 atggtctgat tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt
    33841 atagaggtgt accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag
    33901 cgctagaaca attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta
    33961 gaggtttaga tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag
    34021 aagcttattt gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta
    34081 cactcgtaca agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa
    34141 cacttgaaga gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag
    34201 atgcagctcc tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc
    34261 cagaatataa caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa
    34321 ataaaggagc aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa
    34381 aattggttgc cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt
    34441 gacagaaaac caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg
    34501 aagcaatgca agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc
    34561 tggccgacca gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt
    34621 cgactcttct acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt
    34681 cagcatacag caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat
    34741 ttttggagaa actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat
    34801 cgattcagag atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatgc
    34861 tcaaaatccg caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg
    34921 agcagctcaa gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga
    34981 tgagatccac cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg
    35041 tatttaccgt ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg
    35101 ggcgacgact gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc
    35161 aatcaccttg ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct
    35221 ttttatcaaa caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat
    35281 tcttgccttc atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa
    35341 actagtctgt gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat
    35401 tcgaaaagaa gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc
    35461 ggaacgtctc gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt
    35521 tgctgatgtt gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga
    35581 ggaaaaagtc acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat
    35641 ttcaaaagat gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat
    35701 tcccaaagat cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc
    35761 tgaaaaacgg ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct
    35821 gcaaagtaca ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa
    35881 cgtacggaaa aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga
    35941 agaagaagaa caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc
    36001 tatcaatcta ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat
    36061 gcacggcaga cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa
    36121 agtagggaaa gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt
    36181 gttacaatat gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga
    36241 tatgggatca ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa
    36301 agctatcacg atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg
    36361 aagaagctta gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga
    36421 tcagttcaaa gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg
    36481 ctttactggt gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca
    36541 aaataaggta gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat
    36601 cgatgcctta ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta
    36661 tcaaaatatc ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt
    36721 aaagcgtata gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc
    36781 attaagcgat gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca
    36841 gatccagacc gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca
    36901 tttagccttc atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact
    36961 gtctattttc tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc
    37021 actagaacga aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat
    37081 gtttcttgaa aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca
    37141 tctgtgtgcg cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt
    37201 tttattggca tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa
    37261 tggcaaaaaa aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca
    37321 caaaaatgca aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg
    37381 cctcagatgc agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc
    37441 aagtacgttt catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag
    37501 atgtcatcaa tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag
    37561 aaaatctaat gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca
    37621 aaatctagaa gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat
    37681 ggaagcgatt gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga
    37741 tgcagaagaa tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc
    37801 acaaggaaat catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac
    37861 atctattgcg tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag
    37921 cgaataaata caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat
    37981 tttgttataa ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac
    38041 ggactatcaa gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa
    38101 aaacatttag ttgctttgcg tgatgctttt atcggcacac tgcccgcaac aatggcggga
    38161 tcagtcgctg taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc
    38221 tatgctgaca gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga
    38281 atcaatggtt ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct
    38341 tgggggtata atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt
    38401 ttagctacct tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg
    38461 aatgttcctt caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca
    38521 gatggcacac tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac
    38581 gcttatttta cagttatgat catgggagct atttcagtca ttattttttg taaattgatg
    38641 cttgccaaca ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt
    38701 gcagccattt taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta
    38761 ggtcgtgtag ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagaacca
    38821 ttcctaggat tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta
    38881 ttctggttct ttggtattca tggatcaaat gtattggcac cagtgctaga gggaatctgg
    38941 ggacaagcac aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt
    39001 acaccagccg tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg
    39061 ttcgatgctt ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc
    39121 ttgattttct ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt
    39181 atcttcaata tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg
    39241 tttatcccat tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat
    39301 cttggcctag tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg
    39361 tcattcttag caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc
    39421 gtgactttct tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca
    39481 ggcgaacaac aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat
    39541 gaattttcga ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat
    39601 caaagaattg ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca
    39661 aggaatcaag ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc
    39721 aaaggaacta atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt
    39781 ttagaaaatg gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg
    39841 tagtattggt tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt
    39901 gtatagtatg gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg
    39961 gaaaaaaaga gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat
    40021 attccagcat cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa
    40081 gcgaccggca aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc
    40141 tcgcaaaata accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag
    40201 tttcgtacat cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac
    40261 gattcgacct gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag
    40321 ttgaatgata tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa
    40381 atgctagata tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt
    40441 gaaaaatgaa gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc
    40501 tcactagctt ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg
    40561 tatcttcgat tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac
    40621 ctgaaaggcg agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg
    40681 atgaggagga tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg
    40741 gacaagagta cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac
    40801 aaggcgggat caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa
    40861 gcttatctta tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat
    40921 catttgataa aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat
    40981 ctggaaaaat aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg
    41041 aacagatgcc ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata
    41101 aattcaagca aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa
    41161 cgggatatat cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt
    41221 tggatcatga tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg
    41281 aaacatttga atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc
    41341 catttgcttc tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt
    41401 ttttggaaaa ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag
    41461 atcattatgc gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc
    41521 atctctcaaa agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca
    41581 cgcagctatt attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg
    41641 tgacaaaaga atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta
    41701 atgactattt ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt
    41761 tcatttggca tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt
    41821 tttgttttct tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat
    41881 cttttcccga ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt
    41941 atcattcatt ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt
    42001 taaggactag tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt
    42061 gcaagtaaaa aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag
    42121 gatagagcaa tcgcctccta agcgataggt cgctggttca attccagtca ggaacgtata
    42181 aataacccct gtatcctttg tggtacaggg gtttgttttg tatttgtgcc aaaaatgtgc
    42241 caaactagtt atcattttta tataaactat cgaacacagt attgattttt tctttatcct
    42301 gatcttcaag ctctcgaaca atatgaagat aagtagacat tgtagtttct aaactgctat
    42361 gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat
    42421 gtgtatgcct tagtccatgc aaagtaaatt gcttttcaat ccctaattct tttaattttt
    42481 tgcgcaaata tttgctgacg gcgtttgatg aaactaatcc attttttaag ttgaaaaaaa
    42541 caaaattatg agggttttta acttcaaaat tctcgaataa ttctttttga ttgattttaa
    42601 acttttttaa gagatcaatc gtatgtttat caattgaaat tttccgattt gaagtctcgt
    42661 tttttgtttt tccccattca tttaatttat aattccatgt tttattaata gtgatgattt
    42721 gatcttcgaa atcgatatct tcccatgtta atcctagaag ttcagcaaat cgcattccag
    42781 tggcaccagc aactaacacg agcataggag aagagtactg cgcagaaagg ttttcttcta
    42841 cgagtttcat taaagattta aattgatcat aatctaaata tttatcttct tttggcttca
    42901 atgaatcttt tcctttgatt actgcttttc tagttggatc aaacggaatt aatccttctt
    42961 ctacggcatc tttcaacgac gctctaatat ggttattaaa cttaataacg gtagatttta
    43021 catggtcttt agcatactta tttaaaaagc gttgatatcc cactctatct aaatcagaaa
    43081 tcaataccgc tggcatatat ttctttatgt tcattaacgt atcttcgtat tttctataag
    43141 tgataggaga tactgttcct tctttataaa gttgcatcca atcctcaaag taatctgata
    43201 ataatagatt tttgcgatcc cctttaaggc ctttgttcaa gttatattct aattcgttgg
    43261 cggcatcttt tgcttctcct tttgtcttaa atcctgattt tcttatcttc gtgtattttc
    43321 cgtcatcttt tttgtatgaa atttcgtatt gccaactatt tccacgtttg actaatcttg
    43381 ccataattga atcagctctc tttctctgat acaataggaa ctataaagaa gcccattgtt
    43441 tgggttttat tattttttgc acatcctttt tgcttgccgg cgcaaggatg tgctatttgt
    43501 cgttttaatg gtataattgg atgtgtatta gatcatccaa tcagaggtgt ttattatgat
    43561 taaagtgatt tttaaaagaa taactgaact tccagagttt agattatttg ttgattattg
    43621 gtatgtctgg ttttttattg ttgcattgct gataggtcta gatgtcatat tagaaagaaa
    43681 acttaagtaa gagttgagtt aataaattga attcctaaat gagatatttt gtagcccata
    43741 ttttgtttgc cgacatcacc taatactatg gagctaatcg ttttttctaa cagaccaaaa
    43801 tcagataatc tatttaagct accatctatt agctcttgtg aatagccaga catgctgata
    43861 tttccagtga aattttcgtt tccacgactc attaagcatg ctaacaattg aatctcaatc
    43921 gatgttaatt gattcagtac aagaagataa tactcctcgt tatcccatga agcgctgtta
    43981 attcgaagta aacaattctt ataaagattt ttatacatat ctattttatt ttgtgccttt
    44041 gatttttcta cctcgtcatt tatcccttcg aatattccaa tgagttggtc cttgttctct
    44101 aagttgaaaa catcttctgg aatttgatcg cgaatgtttt ctaatgattc gtttaaattt
    44161 ttgtagaatt tctcgatgcg tttgaaacgc ttctcgtttt tagacccaaa ataaagtgac
    44221 tgcacaacgc caccaatata aggaattgct ccaataccgc cttcaactat taattcaaga
    44281 tattcttttt tggttagttt ttcattgcta ttatccatag ttacctccta ttttgtcatt
    44341 tcctttacaa gcattgtgac tcctgatggc actcttttgt ataggtttta ttattgtgca
    44401 caatctaact cttagtgggg cgtggttcat ttatttttta cttactttac tattaatcat
    44461 atcccattga gcataggatt tttctatgtt cgatcctctt gaactaattt ctcctgtaaa
    44521 agttacttta tcgccctttg taaaggtatc agcattgttt aagtccttcg caaaaattac
    44581 atatgaaact ttaggtgttg cagaaatatc attccaactt ttatcatcgt actctttgtc
    44641 tgctataatg gctattctag tagtcattga ttcgataaca gttccttcaa aagtatatgt
    44701 tttaccacta atatatttag taaaagctgt attttgctca gtagctggag taatagaata
    44761 ataactatct aagaaagcac cccagtcatg attatttgtc tctaaaaatt cttcaaattg
    44821 agttaggctt ttattgctgc tgttagatgt agtagtttga gtatcagatg atgagctttc
    44881 ctgtttgtta ttgtcatttg cctcgtttga tgaggaaagc gagaaaccta cggtagtaaa
    44941 aataatcccg ataataagga gaatcatagg tgttttctta tttgtttttt tgataaatct
    45001 tacaattaac atgattaaac ctataataaa taaaacaata ccagtaaaac caaatatacc
    45061 gtccattaat agatcctctt tctcttttga tattgaatat tataaaccaa gcaattcttt
    45121 tttcttaaaa tcgaattctt cttgattaat aatcccttca tctaacaact ctttatactt
    45181 tttaatttca tctgcacttg aaatttgtgt ttctttggaa ttattatcta atatagattg
    45241 ttgcttctct atatattttt ttaaattttc catttcattc caatattttt ttgtaaacat
    45301 aacggtgttt tcatctttcg tggccgctaa tatacctccg cgactttcgt ttccgcctag
    45361 caaggtaaat tgtatataac cattactcat acctggtttt tttaattgta cggctgatat
    45421 atttttaaag gggatagttt tttctccttt aataccttga ttaactaaat ttataaatcc
    45481 ttttctaata attgaaatag aatcatcagt tactttaatc aaggtttttc ctggagattt
    45541 tattaaaatc tctttttcca ttttttcttc ctcatttctg tgatatgatt ttttttgtag
    45601 aagctcatat atgaggaaag agcccgtgtt gcagcacggg cttttttaat gttttgctga
    45661 aatcggtttt tttaataatt cttgatgtat tcatacatat tgccttgcgt aagaatattt
    45721 tttcttaaaa tagcattggc agacagcata acaagtgtat ctgagcttat tctgaataga
    45781 ataatactcc ataaattttt cgagattaaa ctgagattca tcagtcagtt cgttctcaat
    45841 atagatattt aataaaatta gaatagctat tttatctgct tctgtttcaa attttgagtg
    45901 aaaagttgta gaagtatcgt acaaaactga aaattcaaaa tgagaggcgc tgaaatgtgc
    45961 aagttcatga gataaatgaa aggcttctgc agtttcaccg tatagatttt cattcaaaaa
    46021 aatgattctg ggttttggat agtagaaacc aggctctttc atctccatat aaactacttt
    46081 caaattgtat tcgctcaaca tttctttcaa tttcaaatac atacaaacca tcactccaac
    46141 tattcatttt cctctaaagc tttagcaatt gcaatcgctt tacgcattgt ctccttagat
    46201 atttcttttc catcaaaaga aaaaacagta tcgtcttctg ataaatccac atgtttaggg
    46261 gtttctcttt cttctctacc tagaaggtag tctacagaga catcgaaata gtcagcaatc
    46321 cgacttaatt catctgaatt aggagtatta tttttccact ttgctagata accgttagaa
    46381 tatccaaaat taatttctaa ctgccgtata gatatctttt tcctcttcgc caagtctttt
    46441 attatttcat aggtattcat cgataaaaca acctttctaa atgtttacaa aaaaagttta
    46501 gaaaaatacg cagaaataat ttgactaatt ctgagtaatg agctatacta tatcttgtaa
    46561 acagatttaa caactaaaaa gacaacaaaa aacactattg atatgtaaat gcagaccgcc
    46621 aagaaagctt taaaatcaat gtttttatgt cttatttaat tatgctctta gtatagagta
    46681 ttactcagat actgtcaatt gaatttagaa aaaagttgtt aaatttgttt acgaatataa
    46741 aagaaaggaa tgagaaatat gaacacaccg caaattttta atttcgaaca aaacgaagtt
    46801 cggacgtttc tagaaaatga cattccgtat ttcgtagcaa atgatgtcgc taaaacatta
    46861 ggatacaaaa acccgagcga tgctactaat aaacattgta aaaaagccgt aaaaacatgg
    46921 ggtagcgatt cgctaggtcg tcgccaatct ttcaaagtta ttccagaatc agatgtttac
    46981 cgcttgatta tcaaatcgaa cttaccaagc gctgaaaaat ttgaggcttg ggtaatggaa
    47041 gaagtccttc caacaatcag aaaaacaggt agctattcaa acgtacctca aagttttgca
    47101 caagcattgc gtttagcagc agatttagaa gaaaagaacc aattactcga acaacaaatt
    47161 gccgagtacg aaccaaagat tagctactta gatacgattc tttcatcgac agatacggta
    47221 gcgacttctc aaattgcagc tgattacgga atgtcggcaa ttgctctaaa caaattgctt
    47281 aacgagttag gtgttcaaca taaagttagc ggacaatgga tactttaccg aaaacatatg
    47341 aaccaaggat acacaaaatc gcacacaagt gagattccga aagccgatgg cggcactaaa
    47401 gttgtaatga ataccaaatg gacacagaaa gggcgagtgt ttatttacaa cttattaatc
    47461 gcagagggct attaccctca aatggattta gaataaattg gttagaaagg agttttagta
    47521 tgactgatat tgcagaaatc actcaacgag atagagaaaa aatcaaagca tatgtcgaaa
    47581 gttcgaagtt cttaacttac accatgcttg ctgaaagatt tggaattagt aaaagctact
    47641 tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga
    47701 ttatcactat gtacgaattg taaagggagg aacagcaaat gcaatatcta gaagcgaaaa
    47761 tcccaattcc agaaggctat gtaattatct cccaagtgga ttatgaggag ttgaaaaaag
    47821 ctgatgatac tggtagatgg atgacgttgc cagaagtact agaacggatt aacagaaaat
    47881 atgattggtt tacttctaga gttttaaaga acccaagata tagaaatatt atcgatatcg
    47941 aaaaaaacaa aaatggattt gtctattatc cagttgaagg aagagacaca tatctatttt
    48001 taagaagtaa aacacttgaa tttttagaaa caaatttttc ggaaatctta aggaggtaag
    48061 cggatgggaa aatttaatag agcattagtg ttcagcgcac ccctaatcat ctacgcttta
    48121 ggactttggg gaagcagaca agcattaatc ggcacgatcg tttacatggt ttggattttt
    48181 atggggcttg atgaagctga gtacagagca aaaaaagcca gtcgggaggg actgactaga
    48241 tgagaaacaa aaaaatatct ctaacgctat taactatcat cacgattgtc gtatcgcaac
    48301 tattcttttt aactatatta aacgacatat cgatgtcgga ccgattgttg gttcaatcag
    48361 ctttatttct gacctttgaa aaaatgggta attacgaaag atagaatttg agaaattgtt
    48421 tcttgggcaa ataacgatgt tacccaaatt acaagactaa aaattcttcc tggaaaagtt
    48481 gatagattaa tgccgaacca actaagtatt tcgctgggta agagaaaaaa tttcttaaga
    48541 gcaatcaacg gatttaaaaa ccagaatttt cgataatgaa tttcgttgtt tttgtcaaaa
    48601 atttgagaag cgataattct aaaattttct tcaagaatat catctgattg attcaaggaa
    48661 agcgttacaa ggctataagg cagaactttg ttaattctgg gcatttcctt aaccattatt
    48721 tctctaatag gcgtcacatt attttgaaag taaggatcag atttatagct accactatgg
    48781 tcatttaatc gtttgaggtc atttatcact ttctcaattg tagtaagtaa atagttaatc
    48841 gttgttattt cagacactgc attaacaatt tgcttaatat aaattattgc ataaacaact
    48901 agaagcacat acaaaatgta gttcattgaa aacaccacca tttttttacc taaattatac
    48961 caaaaggaga gaagaaaaaa tgcaagaatt agtaattttg aaaaataaag aagctgtgac
    49021 tacgagctta caagtggcag aaagctttga gaaaaaacat caacatgttt taagagatat
    49081 tgatgcacta aaaaaagatg tgtccaattt tggacagatg tttgtagaag gtaatgaacc
    49141 agattcatac ggcagaaatc gacgagtttt tttcattagt agagatggtt ttttcttgtt
    49201 ggctatgggt tttacaggaa agaaagctat ttacttcaaa caaaaataca ttgaagcatt
    49261 caacgaaatg gaagatgtta ttcgcaagaa tactgttcct caaacaattg aagacatgat
    49321 gatctaccaa ctagaagaaa tgaaagatgt taaaaaagat gtttccatgc ttaaaaatac
    49381 tatgcgaatt agcggacaac aagagtttga aattaagcaa aaaggaaata tgaaagttat
    49441 ggaagttcta ggaggcaaag aaagccgagc ttatgaagaa atcagtaaaa aagtattctc
    49501 aaaattttgg tctgaattta aacgtacctt ttcaatccca agatatggcg agttacctcg
    49561 taaaagattc gatgatgctg tttcatttat tgaaatgtgg ttaccagaaa ctgcgatccg
    49621 catggaaatc gatcaactga acagacaaca aagacttttc ggtgatgaaa atgaatagag
    49681 ctgaagcgct aagaataggg acggtaattg ctaatcgctg gtggagacac aataaaccaa
    49741 gcatcctaag ccaacaacat attgataagc aaaaagcttg gcaacaaata aaaaagtgac
    49801 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    49861 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    49921 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    49981 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    50041 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    50101 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    50161 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    50221 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    50281 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    50341 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    50401 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    50461 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    50521 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    50581 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    50641 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    50701 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    50761 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    50821 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    50881 gctgtcttga tcggtggcca aaaattcatc tggaagcgga ttgaacgaga tcaagagctg
    50941 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    51001 gagcctgtaa ttgacggaag tgaagcgact gctgacttct taaaagaaaa atatgcagat
    51061 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    51121 gaactcaagc ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga caacgagatc
    51181 atcagtgagc taggtaaacg tgaagccagt atcggtatca cacaaagaaa cattatcagc
    51241 tggaaacttg ttcgtacaag acggatgaac acgaagaaac tagcagagaa atatccagat
    51301 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaaggag
    51361 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    51421 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    51481 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    51541 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    51601 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    51661 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    51721 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    51781 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    51841 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    51901 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    51961 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    52021 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    52081 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    52141 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    52201 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    52261 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    52321 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    52381 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    52441 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    52501 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    52561 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    52621 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    52681 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    52741 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    52801 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    52861 tctgcaaagt tgggcagatg acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    52921 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    52981 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    53041 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    53101 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    53161 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    53221 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    53281 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    53341 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    53401 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    53461 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    53521 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    53581 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    53641 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    53701 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    53761 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    53821 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    53881 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    53941 ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc
    54001 gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga
    54061 gtcgaatggt tcggaagata agtaagctaa tcggtgcaga tacgatggat cgatccaagc
    54121 cattacccaa actgaatttt gaatcattta cttttgagaa atatagccat ctgagagagg
    54181 taggctatag tgtaaatgct attcgaaaag cacttggggt aggaagtagt gtatgggcaa
    54241 aatggcggaa agaaaatgta aatgaggtaa atcaaaatga ttaagctaat aggcaaagag
    54301 acgctcaagg tttactatgt agcggaaaca aaggcagaag tcagccgttg gctattgaaa
    54361 atgtttaata agactagagc caatgcttca gtaagtcaga aagcaagtgt atcagaaaca
    54421 ggcatgcctg aagcaatgtg gatttcaagg aggaactgag atggcaatga taccgaagtt
    54481 tagagcgtgg tacacaccat ttaaaggtaa aacaattgga caagaaatga aatatgggca
    54541 agcaggaagg ttgatcactc atgctgaaat ggctccagat aaatatgtgc ttatgcaatc
    54601 cacaggactg aaagacaaga acggtgtggg gatatttgaa ggggatgtag ttcttgtcaa
    54661 tgtaagtaat ggttttgatc atttagttaa tgaaaaaacg gttgtacaag aatccgagtt
    54721 tcattctgga ttagtttgta aatcattaat tagtggaatg gaatatagag tattcaaaca
    54781 caaaaagatg gattatgaat atgaagtcat cggaaatatt tacgagaatt cagagttatt
    54841 ggaggtagag tgattatctt agctactgac tacatcgatt ctttaaaaga tgaagatggg
    54901 aatgtcccag cattgctaaa ttgtggaatt aaaagtaaga atagaatcat tttgttacgg
    54961 atatcggctg atttagcaaa atatctttat ctgagcaaga cagcagtata ttacactgcg
    55021 gctacacgtt atcagtacaa gggaaaaaca atcagtcagg attattatga tcgtttaatc
    55081 agtcctgaca tgcatggaaa atcagaatca gcgatcaagt tatttggcgc aatagagata
    55141 tatgccgatg attttccaaa tttatggttg aaggaggaac agtaatgaaa tacgaaatac
    55201 cactaagcga agcagatgtt cagtcaatta ttaacggtcg ggaggttaat aaaaaacttt
    55261 cagatggtac tgaaatagtt atcagacaaa gttatttgaa agatatggca gctcctgtat
    55321 taattgatcg ttttaacgtg actgattctg cggtagagaa ccacttaaaa gaatttcgat
    55381 caagtataga cgacactttc agattaggga gttgattgac aatgaacaac agacatcgca
    55441 gaataacaaa actaagaaaa cgggaactga atgtactaaa gacaaagttt gaaaaagaat
    55501 atggaatttc agcagaagaa acatataaag tggcaagtca gtgtgttgct gatgcaagtg
    55561 atgctattcg taagtttgga atttcgatac tagatattaa atgggaggat acagaatgaa
    55621 gatttatgta gtaaagtttg gaaataaatt ttacagaagt gatgaacgtt ctataggggc
    55681 taacacatta tccattgtag acatactcca aagtgcaaaa tggtttgata atctcgaaga
    55741 agctaaccaa gtttcacgac gacttggtgg attaacgcaa gtatatgaac tgatcactat
    55801 cgatcatgag gaggtagagt aatgaaacta aaagacggat tttacgctag cagtcacggt
    55861 atcggcggtt taatgctaga tatgccgaca aagaatccta aaacacgtaa gaaaccaaaa
    55921 gtcaaagtcg gtgacatggt tcgctgcgaa gcagaggagt tcgtttatcc cttcagagga
    55981 tacgttaaaa agatactgtc aaactcagca atcattcgta ttgaaaacac gatgaaatgt
    56041 gacaagtggt tagcgaaaag caaagagaat ctagcagtgg tgagattggt ggatatggaa
    56101 gttataaaat cttaattttg cttttttaca actatcctac aacaatgagc gttattccac
    56161 aactggaagg agaagaggca attgtgaaca ttttagagaa tatagatatt aagcaaacaa
    56221 ggaaaaatgc tagacgatta ctaaaaagat acagaaattt agaacgtttg gtcggaccag
    56281 tgaaaataga cttttccgtg atgactgtta ccaaaaattt gaaattcaca attgacagtc
    56341 aaaacgaaga aatcattgaa gcgataagta ctcgagattc ggttatcgaa gcattaacac
    56401 ggctaagtag aatccatttc caggtgcttt attatagtta ttgttttcct aataagatgt
    56461 caatgtatca aataggagaa aaattaggat attccgatag aactatcgag agaatgaagg
    56521 cagtagcttt ggttgaattt gctgaggcgt ataaatcagg agaactcatt tcacgtacaa
    56581 aataaaaaaa gtcggattcc tccgactatg aataatattt ccgacataag tattatatca
    56641 taattggggg aatcagagga tggtactttt cgatgtaaag aaatatgaga caccaagcgc
    56701 gaaagatgtt gatatggaac gcacaaaaca taatgtcgct gtgtttcttt cagcatatct
    56761 atcagctaga tgtagagtag ggcaacctcg agaaccaaaa gtgacagcat cttattcctt
    56821 ggttccacct tctacagctg atcatatatt tgaagcagaa agaatgctga tcgataaaga
    56881 agaagcacag gaagaatttg agtatctgca taaattgttt gttcgaggct attcagcgat
    56941 acagcatccg cataagcctg atgtgactga aaggcgcaag aagatattct atgatcgtta
    57001 tatcaatggt ctgcccattt atgtaactgc tcaaaggaat aatactagcg aagaatcggt
    57061 taaagtagaa tcaaacagaa ttatcatcca atttgcttca tcgttagaac tggttgcttt
    57121 caagtagcca gtttttacac tttttatacc cttttattac caatttggtt tccattttat
    57181 acctttttta taccaatcac ttacctattc aacgttgtat tatgatagtg tcgaaagatt
    57241 aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
    57301 gcttcgatag gcagcaacgg aaatattaag aataaggatg tgaatttcaa ctccttctaa
    57361 attgttctta ttatctatca tccgttgctg tctattaatt tatgtattgg aggaaaaacg
    57421 aatggataaa gaaatcaaag caactgtcaa attagatttg actgaactaa aagaactgct
    57481 caacaaggct agtgaccaag tcgaacagtt acaagaagct ttagatgaaa ttgctaattt
    57541 taaaatccaa gtttcttagc agtatatttt atagcagctg cactcatgaa atcagaccaa
    57601 gtatcaaaat ttgtattatc agatacgaat gcatccattt gatcatcagg aatcgcggca
    57661 aacgattctt cagagctgca gtcaaatccg ctaacctcaa agaattcctc aatggagctg
    57721 aatttggtat tagctgaaac aaaatcattt gtaaataact cggacattgg aacgctatgt
    57781 tcaccgtcca tttcttgggc agcttttgcc attttgttaa gttttttact taaatcatca
    57841 aacccgttac tcatgttatc accacctata atttatttca gcggaccact cgctgataaa
    57901 taaaattata cgcttagtat ttattttcac aatattaatt tatcgtaata acttttgagt
    57961 aaatagaaca aaaaacctgc actagtttcc gctagtacag gcagtgacta tatcggttga
    58021 taatctagca tatattaaaa ataattgcaa gaaaagaaat ttatagtggt ttataccaaa
    58081 ttatcaaagc aatgttattt tgttgctgtc tattgttttt taattattca cacgataact
    58141 aaaggtgggt gaagagaaat gattccatta ataatttcaa tttttgcgct ctgtcttaat
    58201 gtctatatga ttggatttaa aaatgggcaa aataaaaaat agtagcagcc aagaataatt
    58261 ttatagtgtc gagtgtcact gtggcggaaa tagaagacac aacggtaaat gacgagtagc
    58321 ctcgtgagag cctagtaagt tctcgtgtgt ggtgcgattc cactccagtg actttgggtt
    58381 tgcggtacaa gatcccgaaa gtaaaaccgc tagcaaccga gggttggaaa tgggcgctca
    58441 aagtacacga gcaaggcgag gtcgatagta atcgatggaa tcggtgtagg ttgctattac
    58501 atagttggtt aggttagatt gagttttggg atttggtaca aatgaatcgt caaatgactc
    58561 aagcacagga tcggaaacgt ccctgcctgt gcattacata ttagatcact ctttgagtgg
    58621 tctttttatt ttgaaaaaag gaggtaacaa caatgtatag accacaatac ttagaacaga
    58681 agcatgaagt aatcactgtg caaaacggta acggtgagat agtacgaaag tatagaagac
    58741 caataaagag cgatacatat aaacggaagg aaagcaatga agttattcca ttgtatggca
    58801 aaagaatagc taagtattaa ataagattgc gaaaggagat gggacatgac cgaggaattc
    58861 tatagatggt tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct
    58921 aaatggcgca ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt
    58981 agaagactag gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat
    59041 agacctgact tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat
    59101 gaagttcatg gcagatatct tacagcatta gataaacaag agaagaagat agaaagcttt
    59161 gctaacttcg atgcaagtga aaggtggtaa gtgcatgatc atcaatgaca atggcagaga
    59221 gtatgataca gagaagattg aagagtattc atcttataca caaggattaa ttaaacgttt
    59281 gatatacgtt cgctatgtag gtattaggga tctgttatca gataactgtt gcagtaaata
    59341 caaagtgaat caagtaagag aagcgttgaa taaagataat aacgttgaaa gaataaaaaa
    59401 tatttttgga tatagcattg aagagattaa ttattacatt gacttcgctg aagctttcat
    59461 tccgatggtg agataacccc ccttaaaata aatcgcaaat tttttggggg tgatgaaacg
    59521 gagggggctg tcaggaaaag agattttttc gaactttatc atgaaaggag ggctaaaatg
    59581 tttaaaaacg aattgtctca aaatcgctac agagaaaaat tacgccgctc tttaataagc
    59641 caattggaaa gtcagaaaac aaatattgag ccattcttag ataatgttga tcgttatatc
    59701 agtttatggg aaacggcgat atcactggaa gaagatatat ccgagaacgg cattagactg
    59761 gagaatggta aaaagaatga atcagtagcg ttgcttgttt ctgtcaacaa acaaatggga
    59821 ttgatgttgg ataaacttgc cattactcct gaattggtag gtgaagcaaa tgaatcaatt
    59881 cctgagttat aagcatattg aaaattggtt caaagctata gaagaaggca ctatcaaggt
    59941 atgcaaagag caattattgc taaaaaatta tctagaagaa agagtcttta ctagagaaga
    60001 tatttacttc gataagcaga tggtagagga ttcaatcaat ataccagcac aatactttcc
    60061 attcgaatta attccgtggg aaaaatttct acaatgtttt atttatggtg ttcgatggaa
    60121 aaaagataaa acactagtgt tcaatagata tctttcatta atgggacgtg gtaatggtaa
    60181 aactggtttt gcttcttgga acaacttctt tctactaacc gctaaacacg gtattaaaaa
    60241 ttatgatatt gatatctatg ccaataatga aagccaagca aagactagtt ttgatgatgt
    60301 atttaaagta attaaagatc atcctgattt agataaaaaa gtatttaaag ctacgaagga
    60361 agttattcaa aatatcgcta caaacagcaa acttcgttat aacacggcaa atgctagaac
    60421 aaaagatggg aagcgaccag gtgcaaaccg ctttgatgaa attcacgaaa atgaagatta
    60481 ttcaatgata aatgtggcta cttctggtgg tggtaaaatt cgagattata gagaatttta
    60541 tgatacaact aatggtcatg ttcgtggtgg tccgcttgat gacattatag aagaatcaaa
    60601 aatgattctt tctggagaac ttggaattga caaggatgga gcagaatttt ctagtttgtt
    60661 tccatttatt tgtcgcttgg ataacgataa tgaagttgat gatcccgaca tgtgggaaaa
    60721 agcttgtcca actattaatt acaatgcaga tttaaaacgg aaaatgtttc aagaatactc
    60781 tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg accaaacgaa tgaacagacc
    60841 tatggaagat acacgatttg ctgttgcttc atatgatgat gttctgcata cgaaagaaaa
    60901 agaatttcct gaaaaaatgg atgaagtgat aggaacagtc gattttgctg atagacgaga
    60961 ttttgccagc gttgggttgc taggaaaata cgataaagat gtttatttta cacaacatac
    61021 ttttatccac gaatcagccc ttcgattaca aaacatcaaa cgagaggtta tagatatttc
    61081 tatagatcaa ggaaaatcac agatcgttca tggaaaaaat atagaagctg attatattgt
    61141 aggttggttt cttgaaatga gtaataaata ttatattaaa aaaatcgcta tggatatgta
    61201 ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt tttactgtgg aaattgttcg
    61261 aagcggatct gttacacatg gtatgttaaa agatctggtt gatgaccttt ttattaatca
    61321 acgtttattt tttggtgacg atgcgattat gcgttggtat tgcatgaatg tatatgaaga
    61381 gcatatttct aatggaaata tacgctatga aaaaatagaa cctgaaacta gaaaaacgga
    61441 tggctttttt tcattccttc atggtttgaa ttttttagat gatatttatg attctgctcc
    61501 tgtaacagtc acaaatagct cagtagaaaa tacaggaact ggatttactc ctctagtatt
    61561 ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa ggcggtagga tacttcacaa
    61621 aaaaagcaac ggttccttta gaagaatact tttgtaaatt gcaagttgat tttgtgtatc
    61681 gaaaatttgc gattgaaact tgtattgatt tgattgcaaa tgcgatgagt aaagcggaat
    61741 tcaagtcata tgaagatgga aaaaataaaa agaatgatct ttactatagg ctgaatgtag
    61801 ctcctaataa gaaaaataat gcaacagaat ttagaaaaaa actgatcagg agattaatat
    61861 tctacaatga agtattgatc gtttctccgt ctaataattc tagcgaaata tttattgcgg
    61921 atagttggga tgtcacagaa tatgcattga aagatgatgt gttttctcaa gtgcaaatta
    61981 acaacatagt ccttgataga gaatttctag aaagtgatgt tatctatata aaatacgcag
    62041 atcaacaaat taggcaacta gtcgatgcgt attatcaagc gtatgggaaa ctcatttcta
    62101 gtgccatgaa tgtttacaag cgctctaacg ctcgtagata cgtactgaaa gggaatttat
    62161 tccgaccgca agacaataca acacaagatc aaatcaataa aatgatgaca tcacaattta
    62221 aggattttat ggaagctgat aatgcaggtg cggtatttca attacaaaat gagtacacat
    62281 tagaagattt cagcggaaac ttccaaagca attcaagaga tataaaaaac ttaatagacg
    62341 acatctttga gatgacagca gcagcgtttc acgttccgaa aaacctacta aagggagaca
    62401 tgagtgggtt atcggatcaa gtggacgctt ttttaatgtt cgaaatcata ccgattgctg
    62461 aacttattca ggatgcgttt aacgctagtc tctatgaagt agaagaatac ttgtcaggga
    62521 attttgtacg tgtggataca actatgatca agattactag cttcaaagat ttggttgacg
    62581 ctattgatgt aggcattaga aatggagtat ttacaatcaa cgaaggaaga gaacgcgttg
    62641 gaaatgatcg ctctgataag gcgatggcag atgaaatatt tataactaaa aacaatcaac
    62701 aagtatcgaa aggaggtgag gcgaatgacg acaatgaaaa catttctagc agtaaagaat
    62761 gaaggcgcag taccgcaaat ttttattcag ggatttattg gttctagttg gttctttgaa
    62821 gggaatactg acaagggaat caaaaatatt ttggatagtc taggtgatca agaagaaatt
    62881 gaagtagtaa ttaattcaaa cggtggagac gtatttcaag ggattgctat tgggaactta
    62941 cttaagtcaa ataaagcaaa agttaacgtt gtgattaacg gcttagccgc tagtgctgct
    63001 tcaattatcg caatggctgg cgatactata aaaatctaca acaatgcaca attgatgatt
    63061 caccgcgctt ccacatacgg agaaggaaat gtcgatgact tccgtacgat tgctgaccaa
    63121 ctggaatcaa ttgataaatc ggtaaaggct tcatataaaa cacgattcaa tggcacagat
    63181 gaagcattgc aagaacttct tgaaaaagaa tcgtttatgg atgcagaaac agctttgagt
    63241 tatggattgg tcgatgaaat tatcgatgca gaaaatagct caggtactga agctaaaaaa
    63301 gaacaaagcg ttgaagaaat tttgaatgac gttaaagaaa aaagagcaga aaaaattgct
    63361 gcatttacag cagcattaaa taaaacattt ggacaaggag atgcaaaata atgacaatta
    63421 aaaatttaaa aggtgtaaca gctgcaagcg accaattgat gaaagctttt aaagatggta
    63481 acgaagaatc ttttagcgca gctatggtaa gcttatctaa ggaaattcag gataaaattt
    63541 tagaagaagc aacagcaaaa aatcaagatc aattagtatt aatgaaccgt ggtcagcgtg
    63601 tattaactac acaagaaaca aaattctata acgaagtagt gaaaaacgaa ggttttgcag
    63661 gggtcgaaga attagtgcca gctactgtat ttgaacgtgt atttgaagat ttagaacaat
    63721 ctcatccact attgcaaaaa attacttttg ttaacacaac tggtgtaaca gaatggattg
    63781 tgtcacgtgg agtcaatcca gcatggtggg gtaaactttg cgaagctgtt aaaaaagttt
    63841 tagataatgg ctttgatgta attaacatga agcagttcaa gctatcaggt tatattcctg
    63901 tatgtaaggc aatgcttgat ttaggtccag tatggttaga tcgttatgtc cgtactgttt
    63961 tagtagaatc attgagaatt gcattagaac aagcaattgt tgatggtact ggtaaagata
    64021 tgccagtcgg aatgatgcgt gacatgagca aacaaactag cggagaatat gctgaaaaaa
    64081 aagcagaacc tattacagct ttagatgctg taactatggg cggtttgatg gcgcgactat
    64141 caaaattcaa tatcgaaggt gtgaatgatc cgatttatcg taatgtgaat ccttctgatg
    64201 tggtcctaat tgtgaatcca actgattact ggtctaaagt attcccagct aagactgtac
    64261 taactgctaa tggagaatac gtacaagtat tgccagtacc agtttcagat ttgcagtcaa
    64321 cggctgtgcc agaaggaaaa gcagttattg gggtagcctc agattacttc atgggtgtag
    64381 gatctacact aaaaattgaa gcttcagatg aataccattt tgttgaagac gaacgcattt
    64441 atctagctaa acaatatgca aacggtcaac ctaaacgtaa cgatagtttc attgtattag
    64501 atattagcgc tttgggaact actactacaa ctacaaaacc aacaaccaca acaactacaa
    64561 cacaagcgta ggtgatcaga atgaagtata ttctttgtca gccggcaatc aatcggttta
    64621 aatgggagct tgaagtttgt ttaactaatc tgaagaaact aggaatcaaa gatatcgtat
    64681 tgcttttcag cagacacgat gatcagattc ctattttttt tgagaaggaa tatggtgttg
    64741 aagttcatgt gtacgatgat ctgcgggacg acaaagagta tattccttcg attaaaccat
    64801 atttatggtg gaaatattta gaagaagatc attcgcgtga ggacgaccga tatttctata
    64861 tcgattcgga tgtcattttc aataaaagaa ttaatttgcg caaattgcct tctaaagatg
    64921 atgtttggta ttgtagcgac tgctgtagtt atctaagtct tgattatatt agaagctgtg
    64981 aaaacggaga aaatattcta aaagatatgg caaacattgt aaatgttaca gtagaatctt
    65041 tggaaactat aaacactaat tcaggaggcg cacagtgggt tattaaccgt cctaaagcga
    65101 attattggaa aaaggtttat ctggattcta atcggctata tcgctacctt agagggcaaa
    65161 aaacaaatat acaaatctgg acagccgaga tgtgggcaca gctttggaac atgatgtatt
    65221 tcaatattgg tcctaaagtt cacgaggaat tagacttttg ttttgctact gatccaatag
    65281 aaaaagttaa agaagtaaaa atcttacaca atgctggagt aacaacaaac gatgaagatt
    65341 tatttttcaa agggagatac gtgacttcta cgccttttga tgaagattta tcatttgtaa
    65401 acaagaaaaa atgctcttac gcatatgtta aagcaattaa ggcggtggtt agatgacgcc
    65461 tgaacaagtg actgaagaat tgctaatagc tgtgaaggat aatatttacg ttacctggaa
    65521 cgaagaagat gagtcaatta aaaagatgat agctaaaaat gctgtttatc ttcaaagtaa
    65581 agtgagtaca acactttctt tttctcctga aagcttagaa tacggattgc taatcgaaag
    65641 atgtagatac gactggaatc gtgctttaga tgagtttgaa caaaatttcg ctagtgagtt
    65701 attaggtttc attcaacatt atgcgctaca agaatatatt gcaggtgatg tgaatggcga
    65761 ataatcgtag actcgaagaa acattcaacg atggttggtt aaagattttg acgcaaagta
    65821 ccaaaagaaa tgaactagga aaaaagattg gtgtagaaga tacagaaatc acttctttaa
    65881 aatttagaaa tctttccatg agagatagtg atataacagc tatggatgcg atgggatcga
    65941 aattaactaa gaaagtaaag actccatttc atccaatcgc caagaaattt aataaagatc
    66001 aatattttat cgtaatcgat agtatgcgtt acaacgttat ctatgccgat tacgataatt
    66061 tttatatcta tttttatctt gaaagtgtgg gtgaatatgg tgattgataa ttctaaagaa
    66121 aaagaacgtt taaataagca aatttctgct atcaaaactt ccttagaaga gcattttggc
    66181 ctcaaactct ttcaagactc cgtaggcgag gatgagctac ctgatgattt taattacttc
    66241 attctcgaaa caggagaaat agaaatgatc actgagccaa aatatagcgt gggtcaaaat
    66301 ctatatctaa ctttctattc agaaaataga gaagatttaa caggagactc actagatatt
    66361 atttcattga ttcaaaatcg ttcgattcgt tttcagagaa tggatcccaa tcatttaaaa
    66421 ctagagaacc aagatcgcta tatcgatcaa ttggtattta cgtttagacg attattgaag
    66481 agtgattgtc atggctaaaa atagttggga gctaaaaata aatggacatg atgaacttct
    66541 tgtgcggatg gaacgctatt caagcgagag cgaacgactg attaacgaag cattgaaatc
    66601 aaaaggttcg gctattgcag tggataggat tacagaaaaa attcctgttt ctgaagcaga
    66661 tttaagaaga gggcaccaac acgcaaaaaa tagtcgtcca cttaagactc aatacattaa
    66721 tttgggtttc atcattagac ctacaagaaa atttgagtat ttaaaatatc ctgatttggg
    66781 gataggtact tctaaaagaa atcagccgga cgaatttatg agaagaggat taggtcttgc
    66841 acttgatcca attacagaac ttctgattcg tcaattcgat aaattaaata aataggggga
    66901 acaacaatgg ctaaaacaaa aactgtagta acaacgttcg ataacgtaag tatcaaacga
    66961 attgctttta attttaagaa cgcaggaaat gcaatcgcaa cgaattgtaa cggacaatta
    67021 gatggcgaaa cagaaatgca aacggtggtt aaaaaatgtg gagcgacaga agtaaaatca
    67081 aaatctaaac caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat
    67141 cgacgtttca atgggttgaa acaagatgga cgtattaaac caggcattta ctcttacggt
    67201 cctgattccg taggcgaaga tttctcactt gctgcagaga tcgtggatga cttcgaagaa
    67261 aatagaaagt tagttggtat gttagcatgc acttcgaata caggattaac attctctatt
    67321 gaaaatggtg cggatgaagt agctgcgtta gaactagaaa caaaagttat gcaagatgaa
    67381 tttggtaaat tttatcatga agcaattgtt gcagaacttg aagaagactt aacagatcaa
    67441 tggatgacga atctatctgc tgatgtgatt aaaaaaactt cagttgtgac aactacggcc
    67501 actcaatcac agtaaaaaaa acggaggtag cgaaatgaac gaagattact caaaaattga
    67561 actaaacgat ggaacaattt tgaatttaga acctaaactg aatatcaaga aattattgat
    67621 gatcaataga gattttaaca cagacgagtt tgcaaaaatg tcgatgggaa aaggctctat
    67681 ggacattaca gttattcaag gtgcaaaagc cgtatatgta gcttatcgtc aagcgaatat
    67741 ggttgattac atttcattcg atgaatttat cgacaaatgg gattttgata tggaggttgc
    67801 agtagctgta tacagtacta tgatgttcaa acaagcacga gatgcctatc aaaaagaatt
    67861 cgaaaaagca aataaggaaa aaaagcttca aaagtaaaaa tgccaaatct cttagttgaa
    67921 acgtgggtcg atgtctattc gatgttgacc gacgtttttt ctatgccttc agatttggtt
    67981 ttaagcgata tctgtttaga tgacattttg caaatggctt acaacaagag tgcttatgaa
    68041 ggatggaaaa actatgcaat aaaccaatcc cagaaaaact aaagaaagga ggtaaaaaat
    68101 ggctaaaaag agaacagaag cagaagtaac atttatagct aacgatgacg gattgaaatc
    68161 tacgttaaaa gaaatcagcg ctgaattaac taaaaataga gcagaattaa aactagaaca
    68221 agctcaatta caacagactg gttctgaatc agacaagtta ggaagtaaat tatcttcttt
    68281 agagaagcag tatgaattac aaagtcaaaa agttgaagta actagtcaac gtttagccaa
    68341 tgcgaaaaaa tattatggag aaaattccac cgaagttcag aaacttgaga gagaactgat
    68401 taatcaacaa acagcgcaac aaaggttatc taatgatatc gataaaacga gtaaagcatt
    68461 ggctcaatct aaaggtgaat ttaaaaccta tgagtcaact atgaaggatt tagataatga
    68521 gcaacaacac ctaaaatcaa gtgcatcatt agtagaatcc gaatacaaaa aatggcaagc
    68581 aactgctggt cagtctgcta ccgaatctga aaaattagca aaagcacaag aatatgtcgg
    68641 caaacagagt gatattgctg aacaaaaaat cgaagtgcta aaaaaacaat tagatgccac
    68701 acaaaaagaa tttggagaaa catctaccga agctttagat atgaaaacta agcttaatga
    68761 tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa aacgttgata catccacagt
    68821 agacgatatc ggcaaaaaac ttgatatggg caatttaatg gaagctactg atcacttgtc
    68881 agtgatcgga gataagctga ttgatgtagg cagtaagtct attgaagcag ctggaaaagc
    68941 acaagctatg caggcccaat ttaaacaagt ctttggctct ttagaagggg aagcgcagga
    69001 cgccgttgag ggaatggctg aaaaatttgg aatgttacca aatacgatca agcctgtttt
    69061 tacgcaatat acgtcaatgt ttaaaggact tggatatgat acaaaagaag ctatggagtt
    69121 agctggtgac agtactcagt tagcagcaga tgcagcagct ttttacgata agtctatgga
    69181 tgatgctagt gaatctctta attcattcat aaaagggaac tacgaaggtg gagaacaaat
    69241 aggtctattt gctaatgata ctcaaatggc tgcttatgct gttaagcata acttgatacc
    69301 agcgactgaa ggagcaaaaa aagccagtga agagtcattg ttagccgttg aaaaagcaca
    69361 atctaagtat gctgatgcta ttaagaaaca tggcgaagga tctttagaag caagagaggc
    69421 tgctttaaaa cttaaagatg cgcaagataa aataaatgaa gaattaggcc cacaaactca
    69481 aaaatggtct gatttagatg aggctaccaa acaagcagtt cgagttcaat atgctgaaga
    69541 tatgcaaaaa ttagccggtg ctacaggtca ggctagtaga gagtctgatg gtttagaaaa
    69601 ccaaatgaca agagcaaagc aagcattaga agatttttat gcctcgttgg gtgaagatat
    69661 attgcctgtg tttatcaaag ggttacaagc aggcgcgaaa gctttgcaag gattagccga
    69721 atggtggagt aaacttgatg gaccaatgaa aaatttcatt ttagctcttg gaggaattct
    69781 agcattatta agcacattag ctcctgttat aaccgcagtt gttacgatag ttggcacatt
    69841 tggttctaca gttttgctgc caataatagg aattatagcg ggcgttgcag ctgtaatagc
    69901 gattgtaatc acagcgtttc agaactgggg tgcaattact gactggttta agggaatatg
    69961 gagtaaattc actgattggt tgggtggtac ttgggaaagc ataaaagaag gtgcctcatc
    70021 agtttgggat ggagttaaag aaacctggtc tggatttgta gattgggttc aagaaatttg
    70081 gcaaggagtt tctgattggt ttggagagtt atggagcgga ttagttgaag gagcttccaa
    70141 catctggcaa ggagtccaag agacttggca agcattcgtt gattgggttt caaatatttg
    70201 gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat
    70261 accatggaca ttaataacgt cattgattca agctggtatt aatattatcg tgggtatctt
    70321 tgatgtagct ggacagttat taggcgcagc ttggcaagct gtttggacac ctatttctga
    70381 tttccttaaa aatacttggg atactatgac acaatggata agcatcgctt ggaatggaat
    70441 tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac
    70501 atctattagt actacggctt caaatatttg gaactcaatt agtgcaacag cttctagtat
    70561 ttggaatagt atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca
    70621 aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac
    70681 agcatcaaat atctggagta gcgtgactag tagtataagc aatgctataa acgcagctaa
    70741 aagtgccatt caaagtgttt ggaatagtat atcttcgtgg atcagcggaa tttggaacgg
    70801 tatcaaaaac actgctttga atctttggaa tggaattaca agcactatta gctctaaagt
    70861 aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga
    70921 catattcaat aatgttaaaa gtacgattgc taacatttgg gaaggtatta aaaagactgt
    70981 tagcgctccg attgattgga ttagagataa aatcagtggc atctttgata atctgaatat
    71041 ttcgatacca catattccgt taccacattt taaattgagt gggaaattca atccattgaa
    71101 gggaaaaatc ccaacgttgg gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc
    71161 gaatattatc ggtatcggtg aagcaggacc tgaagtagct ttacctttga aaagatctgt
    71221 gctgcaagaa attggggatc gtatcttgag tagcacctca gtttcatcta gggcacaaac
    71281 ggttcaacct gtgaacaatt acgaattcaa tttcacaatt gatggtaacg cagatgaggt
    71341 tactatgaag cagacaactc aacaaatcat tgacagcatt acaaaagttc aaaatgataa
    71401 tgcttcggca tggcgttaaa caggagagta tttctcctgt tttcttagta ttacaaagga
    71461 tgtgaaaaaa tgactgattg tatacattct atagtcgatg gatttcctga ttatttgcat
    71521 aaattggctt tagctgaaag accaaccata ccttctccaa aaagacagag agttgaaact
    71581 tctgttttag gacggctagg tggcttagta caagattatt cgtttgaaga tatgtcgttt
    71641 actttgcact ataactattt agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt
    71701 tatatcatgc gtcattggtt aaattatgca aagaaattag aattctctga tgatcccaac
    71761 gtctattatg ttatccagac tatcgatatt ggggatgcag aaaacgatat tgttgaatgg
    71821 ggagagttcg atgtaaatat cactgcgaaa ccattcgcaa gagttcaaga agatgtacct
    71881 ataaccgtag ataaaccaca gtcatttaac ttgctgaata atagtttaga agaaagtttt
    71941 ccaaagatta tcatcactcc ttcagctact tcatgccaat tcatcttaaa tgattatgtg
    72001 tttagttttg aaggcttagt agtaggaact gacatagtca ttgatagtga tctaatgctt
    72061 tgctacgaag agcaatcgga cggagatatt ttagatcggt ccaacaaaat gaagaccatg
    72121 caatatccga cattgcaagt ggatatcaat cattttaatt gcactggttt gagcaaaata
    72181 caaatttatc gtaatgggtt aaggtaggtg aaatagatga tcgataattt aataactatt
    72241 tacgataaaa atgacgcgaa taatttagct gaacatttat atgatacgca aggtttaggc
    72301 gctttgtcag actggttaac agccactgtt agcaataaac taaacggagc cgagatattt
    72361 cagggtactt atccaataag cggaactaat gcagatttga ttatagaagg acgtattatt
    72421 cagtgctatg tagatgaaaa tcgagcaaag caacgtttac ggatttatta tgcaaagact
    72481 tctgtaatag gaaatacgat agaagtaaaa gctgaaccta ttttcaatga tataagaaaa
    72541 tcggtgttga ataaatatga cagtggaaca gaaaagatca ctgctactca ggcatggcaa
    72601 aacgcaaaag ctttagcgaa accagctatc ccttcgcagt tttctttctc gtcattagta
    72661 gatacgcttg ctaatgtgaa gatagaaaaa gcgaattttt tagaattctt tggtggaaaa
    72721 gagggatcta ttctagatcg atttcatggg gaatttctta aagataataa cacattacgt
    72781 catgaaaaaa ggctaggtac ggatcataaa atcaaagcga tttatactaa aaatttaact
    72841 ggtcttgact tagagataga tgctcaaagc gttttagttg gagtttatcc attcattagc
    72901 agctcttcag aaggagaaga cgagatcact ctaccagaag aagttatttt cacggattac
    72961 gtggatgatt atcctgctgg atatgtttct tttgttgatt ttaaagacaa agcgactgat
    73021 gtagccacat taagggaagc tgctaaagac tggttgaaaa caaacataga taaacaaaaa
    73081 ccacaagtga gtggttcgat tgaattagta ccattgaggc atcaaagagg ctatgaaaaa
    73141 tttgttgatc tagaaaaagt ttcgatgggt gacggagtag atgtgtatca tccacagtta
    73201 aaagtgaata tgtcagcgag aattgtggaa tatacgttta atgttctaac caattcatac
    73261 gataaattag ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga gaataatgtc
    73321 agcaatttga ttaatgatgc cattgatcaa ttgaaaaacg gtggcgaaat cagtgattta
    73381 atcaatgata ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttatgtt
    73441 ttattagatc ctaaagaagc gcctagtcgt attttgatta tggacacacc agataagaat
    73501 accgcaagga atgttttaca aatcaacaat gctggtattg gtttttctaa aactggcatt
    73561 aatggaacat atgaaacggc atggacgtta gatggcggat tcaatgcctc gtttattacg
    73621 gctggtgaaa tagtaggaat tactattaga ggtactacat taattagtga tggtgctgat
    73681 tatagaacaa gtattgctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat
    73741 attatggagc tagaagcacg tgattatgta agtgctgatg ccggtattgt atcatacacc
    73801 atgaaaactg gtggtggttt catgattaga aatccacagg gtaacttggt ttttagtacg
    73861 tgggataatg gtaataacag accgtttcta tcttttggtg cgcccaattt caggtatagc
    73921 aatgctagtt atgtaacttc tggcgacggt agttctttaa gcattaatgg tagtgcgggt
    73981 aactcatggg aatttaaggt agctggtagg actatgaaat ttactagtga tggtatgcta
    74041 acgttaccag gttgtttttt tggttcatgg gaagatggga aacttgctag gtttgaacaa
    74101 tcaacggtac aagtatataa agattttact gttagaggta ctaaaaactc aactgtacca
    74161 acagaacatt atggacaacg actattgaac gcttatgaaa ctccagaata ttatttcgct
    74221 gattatgggg aagccgttac aggtgacgat ggtaaagttc gtgttgatat tgaccccatg
    74281 tttgctgaga cagtaaatct aagtcggtat atgacacatg tgacacctac agaactagtt
    74341 ttgtgtgctg ttactcatga agatattgac catttcatca ttgaaactag taagccaaac
    74401 gtattagtta gatggatttt agtggcacac cgtctagggt atgaagatat tagattaaaa
    74461 gaggatacag catatgatag cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag
    74521 gaggtatata aatggctagc agtttatata atttggcttt agatttcagc aaagaattaa
    74581 actacaccaa agctattatg gctcgtcagg gtgataaagg gattacggtg acggttaaac
    74641 catatttaaa tggcttgcag atggatacga gtggcggaac atttacttta aaaggaacaa
    74701 caccatctaa ccgttacgta gatagtgttg caactagcgt aactagtgaa gaagtcacgt
    74761 tttctcttga tggcacattt atgagtgaag caggatatta taaacactgc tacgtagaat
    74821 atagaaaaga caatcaaatt ttaacaacgc aagatatcat ttttttctca ctaggagtgt
    74881 ctgacatttc gcaaggtcaa gccgatgaat atgtttcgca attagaagag ttgattcgaa
    74941 agtataatga aacttttgat gcttttatgg ctgaaactaa aggtagagtg gatagcttaa
    75001 atcaacagat tactgattta actggtcaag ctaaaacgct acaagacaag ttagatgctc
    75061 tgaaagaaga aaattctaag ttaggtaact tacaagtgat gtacagtaac agcattgatt
    75121 tcgggggcta tgattattcg gggaatccga atttgatggc taatataaac gctgatagct
    75181 tctcgcaagg tagtggtgcc ttatctgttg tagatgatgg tgacgaggta gtgattacgc
    75241 ttgatccaaa ccataaatta gaggtattaa aaccgaaaag tcaaccagcc ctattaacag
    75301 gcaaaacata taccgtgagt gtagaaatta tgttagaagg tgatttcact ggagacccta
    75361 gcaagatagg cctaagatat attaaaatgc ctaactgggt atcagagcta tatacgcgta
    75421 atacattaac tgctactaag ggtgtatggc aaaaactaac tggcaccgtt aaaattactg
    75481 ctgcgagtga taacgctgaa agctggctta taatgctaca aaataaagac gctaataaca
    75541 gcctatccgg caaactacgt ttgagacacg ctaaactcga agaaggctca acagctacac
    75601 cattccaacc taacttatta gcagaacctt acaacatgtg tcgcgaatat cctaacgaaa
    75661 atattgccga tcctacagtt aagttcccaa tcgaatctgg cgaccaccaa atatatcaag
    75721 gttacacaga agaagagctt atgataggtc aaacgtatac tatcacgctt aaaggaacaa
    75781 aacccgcaag tcaaaccttt gtagcgtata atcattggac tgctcgttta ggagaactaa
    75841 agccggttga tgggttgaca gacgtatggt ctctaacatt cacaccaacg aatgttgtgg
    75901 cgatgcctaa acttttccgt gtttatcagt atccacgatc aacagtaggc gcatgccgga
    75961 tcgactggct caagatcgaa aaaggcaaca ctcgaacccc gaatattagt gagtataaat
    76021 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    76081 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    76141 caaacaatta taccgatggg aaagtatcga agattaattc gcagttgact gcttcaatta
    76201 atgaagtaga caccacagct aagggtgctc aaacaaaagc gaatgctaat gcgactgcta
    76261 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accgcatttt
    76321 atggcgcaca aggtgaagtc tctaacaaaa cgaaagtagc ggagtatgga gtaggaacag
    76381 ggtatactac tacagctgta ggagaaacac atttcgaacg tcaatctaac ggatacgtta
    76441 aatgcttaaa agctggtaag tattcaatta gtgcgcaaat acgtgtgcaa ttgggcggga
    76501 aatatgcttc atggttatat actgatttat ataaagatgg aacttctgtt gataatttag
    76561 tatcttatgg ggtcgacgcc ttgcaaaata gatttgcagc tagcggaaac attgtaactg
    76621 atttagaagt aaacgatgta ctaaatatgc gtactgaaat cggcgttgca aatggaattt
    76681 tacaatttac atcttgccgt actttagtct tgactagact atctgattaa tacaatattt
    76741 ataattttgc tatcaacacg ctcaaaggag ggtgtttttt attttgcaat gaaaggagga
    76801 tagttggttg aaagacgaag caatacaaga cgtggtagaa cgcttagtgc gtattgaaac
    76861 gaaactggat aattacgaat tattacgcga aaaagcggaa agtgcaaaag atagagcgga
    76921 tcaggcatat tctattgcgc ttaataatgc ggaagatatc aaagaaatga aagccaataa
    76981 taaatggtcg tggggttaca tgatcggttt aggcattacg atcattggct atttcttgac
    77041 taaattgtaa aggaggtgag aagaaatgat tttacccgat aagtattatc aagtcattaa
    77101 atggacggtt ttaacagtat taccagctgc atctgcttta gtagccatgt taggcaaagc
    77161 ctatggatgg aatggaacag atatgacagt tctgactatc aatgcagtag ccacgttttt
    77221 aggcgttatc actggtgtgt cggcttataa tttgaaaaaa taggaggaaa caaatgaaaa
    77281 agaaaattac tattactgcg atgagcctat taacggctct ttttttattg ccaattaatg
    77341 ggttcgccta tacgattaac aatgaattta atttgggcct aaacgaaggt agctcacaag
    77401 tagcaaataa tcagtacatt ttactgcatg aaacggctaa tgaaacagca acaggacgca
    77461 atgaagcgca atatatgcaa cgttcatgga ctagtgctta tactgcttat attgtgggag
    77521 acggtggaat tgtttaccaa gttggacaac ctggttatgt acagtacggt gctggttcgt
    77581 atgctaatgc taacagtcct gtgcagattg agttacaaca cacacataat aaagcaactt
    77641 ttgaaaagaa ctataaagca tacgttgaat tggcgagaga ttccgctaag aaatacggta
    77701 ttccgcttac attggacact ccttataacc aaccaggaat caaatcacat ttatgggtaa
    77761 cacaaaatat ttggggcgat catacagatc cttacggtta tctttctgaa atgggcgtaa
    77821 gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat ccaacaactt
    77881 ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct acgctgacag
    77941 atggaacaaa tcacgcccac attgatcagt ttggggaaat cgaaaacgca aacttgcatg
    78001 tggctggatg gcacattgct aactataaat acgagtatat tttcattatg gactacaata
    78061 ctgggaaaga attagctcga gtaagagctg atggaattta tagaccagat gtaaaccaag
    78121 cttataatac ttctggaaat gttggttatc atgtatcttt caatatgcgt aattttccta
    78181 gtaagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agagggaaac actaaaggcg
    78241 gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga taaaaatagc
    78301 tcctcgttga ggagca
//
LOCUS       DAPBBG010000005        77463 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPBBG010000005 DAPBBG010000000
VERSION     DAPBBG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 77463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.005"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..611)
                     /locus_tag="RD477_000389"
     CDS             complement(<1..611)
                     /locus_tag="RD477_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7632783.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTA"
     gene            724..1476
                     /gene="xth"
                     /locus_tag="RD477_000390"
     CDS             724..1476
                     /gene="xth"
                     /locus_tag="RD477_000390"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDT7632784.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            2340..2873
                     /locus_tag="RD477_000391"
     CDS             2340..2873
                     /locus_tag="RD477_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDT7632785.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(3363..3887)
                     /locus_tag="RD477_000392"
     CDS             complement(3363..3887)
                     /locus_tag="RD477_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7632786.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(3880..4353)
                     /gene="tsaE"
                     /locus_tag="RD477_000393"
     CDS             complement(3880..4353)
                     /gene="tsaE"
                     /locus_tag="RD477_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDT7632787.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(4476..5456)
                     /gene="pta"
                     /locus_tag="RD477_000394"
     CDS             complement(4476..5456)
                     /gene="pta"
                     /locus_tag="RD477_000394"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDT7632788.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(5609..6289)
                     /locus_tag="RD477_000395"
     CDS             complement(5609..6289)
                     /locus_tag="RD477_000395"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDT7632789.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            6415..7284
                     /locus_tag="RD477_000396"
     CDS             6415..7284
                     /locus_tag="RD477_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7632790.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            7310..7825
                     /locus_tag="RD477_000397"
     CDS             7310..7825
                     /locus_tag="RD477_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632791.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(7872..8465)
                     /locus_tag="RD477_000398"
     CDS             complement(7872..8465)
                     /locus_tag="RD477_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7632792.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(8490..8669)
                     /locus_tag="RD477_000399"
     CDS             complement(8490..8669)
                     /locus_tag="RD477_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632793.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(8746..9255)
                     /locus_tag="RD477_000400"
     CDS             complement(8746..9255)
                     /locus_tag="RD477_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632794.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(9351..9731)
                     /locus_tag="RD477_000401"
     CDS             complement(9351..9731)
                     /locus_tag="RD477_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7632795.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            10061..10810
                     /locus_tag="RD477_000402"
     CDS             10061..10810
                     /locus_tag="RD477_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDT7632796.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(10994..12271)
                     /locus_tag="RD477_000403"
     CDS             complement(10994..12271)
                     /locus_tag="RD477_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632797.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(12350..12994)
                     /gene="pcp"
                     /locus_tag="RD477_000404"
     CDS             complement(12350..12994)
                     /gene="pcp"
                     /locus_tag="RD477_000404"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDT7632798.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(13193..14137)
                     /locus_tag="RD477_000405"
     CDS             complement(13193..14137)
                     /locus_tag="RD477_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDT7632799.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(14139..14825)
                     /locus_tag="RD477_000406"
     CDS             complement(14139..14825)
                     /locus_tag="RD477_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDT7632800.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15299..15619
                     /locus_tag="RD477_000407"
     CDS             15299..15619
                     /locus_tag="RD477_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDT7632801.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            15773..16678
                     /locus_tag="RD477_000408"
     CDS             15773..16678
                     /locus_tag="RD477_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7632802.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            16675..17448
                     /locus_tag="RD477_000409"
     CDS             16675..17448
                     /locus_tag="RD477_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7632803.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(17629..19053)
                     /locus_tag="RD477_000410"
     CDS             complement(17629..19053)
                     /locus_tag="RD477_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7632804.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            19264..19710
                     /gene="nrdI"
                     /locus_tag="RD477_000411"
     CDS             19264..19710
                     /gene="nrdI"
                     /locus_tag="RD477_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDT7632805.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(19775..21757)
                     /locus_tag="RD477_000412"
     CDS             complement(19775..21757)
                     /locus_tag="RD477_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7632806.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(21771..22667)
                     /gene="murQ"
                     /locus_tag="RD477_000413"
     CDS             complement(21771..22667)
                     /gene="murQ"
                     /locus_tag="RD477_000413"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDT7632807.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(22670..23731)
                     /locus_tag="RD477_000414"
     CDS             complement(22670..23731)
                     /locus_tag="RD477_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7632808.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(23942..24508)
                     /locus_tag="RD477_000415"
     CDS             complement(23942..24508)
                     /locus_tag="RD477_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7632809.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(24536..26038)
                     /gene="eat(A)"
                     /locus_tag="RD477_000416"
     CDS             complement(24536..26038)
                     /gene="eat(A)"
                     /locus_tag="RD477_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDT7632810.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(26296..27417)
                     /locus_tag="RD477_000417"
     CDS             complement(26296..27417)
                     /locus_tag="RD477_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7632811.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            27543..28484
                     /locus_tag="RD477_000418"
     CDS             27543..28484
                     /locus_tag="RD477_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDT7632812.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(28634..29530)
                     /locus_tag="RD477_000419"
     CDS             complement(28634..29530)
                     /locus_tag="RD477_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDT7632813.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            29799..31139
                     /locus_tag="RD477_000420"
     CDS             29799..31139
                     /locus_tag="RD477_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7632814.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            31357..31800
                     /locus_tag="RD477_000421"
     CDS             31357..31800
                     /locus_tag="RD477_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDT7632815.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(32304..33722)
                     /gene="nhaC"
                     /locus_tag="RD477_000422"
     CDS             complement(32304..33722)
                     /gene="nhaC"
                     /locus_tag="RD477_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDT7632816.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(34125..35348)
                     /locus_tag="RD477_000423"
     CDS             complement(34125..35348)
                     /locus_tag="RD477_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDT7632817.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(35379..36218)
                     /locus_tag="RD477_000424"
     CDS             complement(35379..36218)
                     /locus_tag="RD477_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDT7632818.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(36235..36915)
                     /locus_tag="RD477_000425"
     CDS             complement(36235..36915)
                     /locus_tag="RD477_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632819.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(36917..37990)
                     /locus_tag="RD477_000426"
     CDS             complement(36917..37990)
                     /locus_tag="RD477_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7632820.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(38046..38284)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(38327..38560)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(38637..39308)
                     /locus_tag="RD477_000427"
     CDS             complement(38637..39308)
                     /locus_tag="RD477_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7632821.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(39395..42073)
                     /gene="mgtA"
                     /locus_tag="RD477_000428"
     CDS             complement(39395..42073)
                     /gene="mgtA"
                     /locus_tag="RD477_000428"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDT7632822.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            42732..43169
                     /gene="ndk"
                     /locus_tag="RD477_000429"
     CDS             42732..43169
                     /gene="ndk"
                     /locus_tag="RD477_000429"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDT7632823.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(43191..44057)
                     /locus_tag="RD477_000430"
     CDS             complement(43191..44057)
                     /locus_tag="RD477_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7632824.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(44217..46310)
                     /locus_tag="RD477_000431"
     CDS             complement(44217..46310)
                     /locus_tag="RD477_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7632825.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(47059..47682)
                     /locus_tag="RD477_000432"
     CDS             complement(47059..47682)
                     /locus_tag="RD477_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632826.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(47841..49238)
                     /locus_tag="RD477_000433"
     CDS             complement(47841..49238)
                     /locus_tag="RD477_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7632827.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(49270..50241)
                     /locus_tag="RD477_000434"
     CDS             complement(49270..50241)
                     /locus_tag="RD477_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7632828.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            50615..51040
                     /locus_tag="RD477_000435"
     CDS             50615..51040
                     /locus_tag="RD477_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632829.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(51528..52229)
                     /locus_tag="RD477_000436"
     CDS             complement(51528..52229)
                     /locus_tag="RD477_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7632830.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(52245..52685)
                     /locus_tag="RD477_000437"
     CDS             complement(52245..52685)
                     /locus_tag="RD477_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDT7632831.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(52685..53260)
                     /locus_tag="RD477_000438"
     CDS             complement(52685..53260)
                     /locus_tag="RD477_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDT7632832.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(54108..54587)
                     /locus_tag="RD477_000439"
                     /pseudo
     CDS             complement(54108..54587)
                     /locus_tag="RD477_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(54746..55447)
                     /locus_tag="RD477_000440"
     CDS             complement(54746..55447)
                     /locus_tag="RD477_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7632833.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(55555..56964)
                     /locus_tag="RD477_000441"
     CDS             complement(55555..56964)
                     /locus_tag="RD477_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDT7632834.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(57012..58877)
                     /locus_tag="RD477_000442"
     CDS             complement(57012..58877)
                     /locus_tag="RD477_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7632835.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(58998..59849)
                     /locus_tag="RD477_000443"
     CDS             complement(58998..59849)
                     /locus_tag="RD477_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7632836.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(60152..60865)
                     /locus_tag="RD477_000444"
     CDS             complement(60152..60865)
                     /locus_tag="RD477_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDT7632837.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(60933..61259)
                     /locus_tag="RD477_000445"
     CDS             complement(60933..61259)
                     /locus_tag="RD477_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDT7632838.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(61382..61609)
                     /locus_tag="RD477_000446"
     CDS             complement(61382..61609)
                     /locus_tag="RD477_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDT7632839.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(61931..63184)
                     /locus_tag="RD477_000447"
     CDS             complement(61931..63184)
                     /locus_tag="RD477_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7632840.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            63245..63985
                     /locus_tag="RD477_000448"
     CDS             63245..63985
                     /locus_tag="RD477_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7632841.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(64071..64829)
                     /locus_tag="RD477_000449"
     CDS             complement(64071..64829)
                     /locus_tag="RD477_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7632842.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(64826..65854)
                     /locus_tag="RD477_000450"
                     /pseudo
     CDS             complement(64826..65854)
                     /locus_tag="RD477_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(65874..69041)
                     /locus_tag="RD477_000451"
     CDS             complement(65874..69041)
                     /locus_tag="RD477_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632843.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(69265..70149)
                     /locus_tag="RD477_000452"
     CDS             complement(69265..70149)
                     /locus_tag="RD477_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDT7632844.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(70244..70681)
                     /locus_tag="RD477_000453"
     CDS             complement(70244..70681)
                     /locus_tag="RD477_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632845.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(70898..71809)
                     /locus_tag="RD477_000454"
     CDS             complement(70898..71809)
                     /locus_tag="RD477_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDT7632846.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(72266..72694)
                     /locus_tag="RD477_000455"
     CDS             complement(72266..72694)
                     /locus_tag="RD477_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632847.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(72722..73360)
                     /locus_tag="RD477_000456"
     CDS             complement(72722..73360)
                     /locus_tag="RD477_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632848.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(73364..75346)
                     /locus_tag="RD477_000457"
     CDS             complement(73364..75346)
                     /locus_tag="RD477_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDT7632849.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            75526..76191
                     /locus_tag="RD477_000458"
     CDS             75526..76191
                     /locus_tag="RD477_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7632850.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        22552 a        16023 c        13279 g        25609 t
ORIGIN      
        1 gccgtttgat tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct
       61 cttatctggt attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata
      121 agatgtaaaa acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt
      181 ttttgcaggt ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca
      241 aagcgccggt tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat
      301 tcacgaactt catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt
      361 tctagacatt ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct
      421 tcgatttttt cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct
      481 ttttgaattc tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct
      541 tgttttttcg cataagttgg taaatatgct ttttctgcga tattccctac accgattaca
      601 ccaatcttca tctactttcc tcctggatca tttagttact ttcatcttac accatttttt
      661 actaaaatga ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt
      721 ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt
      781 ttagaagttt ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa
      841 gcaggacaaa ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga
      901 aaaggctatt ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga
      961 atgggtatcg atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc
     1021 tttcttgtaa cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga
     1081 ttagaatggg aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc
     1141 atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg
     1201 aatcaaaaaa atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat
     1261 aacgggttta tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg
     1321 tggaactacc gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg
     1381 acgagcaaag atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc
     1441 agtgatcatt gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag
     1501 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca
     1561 taataccgag aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct
     1621 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg
     1681 cttattaccg cagatacaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc
     1741 actgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt
     1801 gggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtgaaaaagc
     1861 tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg
     1921 gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa gttgtgaaat
     1981 cagcgtcctg acctgagtat tagagaaaca aatggcaaaa tagttcctca tattttgcta
     2041 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag aagttgtgaa
     2101 aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa atggttcctc acaactttgc
     2161 ataaatttga cttaataccg agaagtcatg atccccatct aactccaaat cacaaggaat
     2221 gagttcaaaa actagctttt ctcaacaaat attacataaa aaattctatg ttttttgtta
     2281 aatttatttg cttttttcaa aagtatgtta cagtaatttc ccaaggaggt tttacagaaa
     2341 tgaattttgt tgccatggat tttgaaactg ccaatcatca gccttatagt gcttgttctc
     2401 ttgcgctagt catggtaaaa aatagccaga tcgttgatga attctataca ttgatacagc
     2461 ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa gaagacgtac
     2521 gcaatgcgcc gaaattccca gaagtctggc aaactatcca accatatttt caaaaaaatc
     2581 gtttagtcgt tgcacataat gctgcttttg atacaaaggt cttagcggga tgcttagatt
     2641 attatcatct tgaacagcct aattatcttt ctttatgtac agtgaaaagt agccgtcgcc
     2701 tgtttcctga atttccaaat catcgattga acactgtgtg cgaaaatttg aacatccaat
     2761 tagatcatca ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc ctgctttatc
     2821 aagaaaaaaa attcgggaca gatccattga aaaaacttgt gactataaag taaaagaata
     2881 cttatgaaac acacaaagag gtcgtgagcc tgccgtttca ctccgagtca caaggagcaa
     2941 tttcaaaaaa tagcttctca tgtttattaa atattgtgac tggtgaccga ggagctggca
     3001 ggagaacacc gtttattgga gaggttgtga aaaagctgtt ctgcatcaag taataagaac
     3061 agccaaacaa aaatagcttc tcatattttt catttggttg gacttattac cgcagatgca
     3121 agcttttgga caccgtttac caagaggttg tgaaatcagc gttttgacct gagtattaga
     3181 gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggtc
     3241 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
     3301 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
     3361 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
     3421 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
     3481 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
     3541 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
     3601 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
     3661 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
     3721 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
     3781 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
     3841 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
     3901 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
     3961 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
     4021 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
     4081 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
     4141 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
     4201 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     4261 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     4321 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     4381 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     4441 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     4501 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     4561 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     4621 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     4681 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     4741 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     4801 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     4861 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     4921 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     4981 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     5041 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     5101 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     5161 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     5221 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     5281 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     5341 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     5401 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     5461 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     5521 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     5581 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     5641 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     5701 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     5761 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     5821 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     5881 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     5941 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     6001 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     6061 ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa
     6121 cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct
     6181 tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa
     6241 attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct
     6301 cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt caccctcagc
     6361 acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc
     6421 aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct
     6481 gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact
     6541 ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg
     6601 atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg
     6661 attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat
     6721 gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt
     6781 gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc
     6841 tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa
     6901 gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta
     6961 ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa
     7021 aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct
     7081 cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc
     7141 agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa
     7201 gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg
     7261 cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt
     7321 tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg
     7381 acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta
     7441 tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg
     7501 ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc
     7561 cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata tctataatca
     7621 tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt
     7681 ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg
     7741 tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt
     7801 ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca
     7861 ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc
     7921 tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca
     7981 tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct
     8041 aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa
     8101 taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga
     8161 tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa
     8221 ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg
     8281 taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct
     8341 gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt
     8401 tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct
     8461 aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta
     8521 atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact
     8581 ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt
     8641 ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc
     8701 ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca
     8761 tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt
     8821 gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt
     8881 catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc
     8941 ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac
     9001 ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt
     9061 ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc
     9121 tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc
     9181 ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa
     9241 catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga
     9301 acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc
     9361 agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta
     9421 tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca
     9481 atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt
     9541 tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat
     9601 ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta
     9661 acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt
     9721 gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag
     9781 ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa
     9841 ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat
     9901 gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt
     9961 tataattgaa agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata
    10021 cagaacacac tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt
    10081 tttagctcat cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct
    10141 tggctgccag gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt
    10201 tatcggtacc acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa
    10261 cggtatttta ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa
    10321 ctatttaagt atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt
    10381 aagtaaagaa tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg
    10441 gtttatcgca atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg
    10501 ttatacagat gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc
    10561 tggtatcatc agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct
    10621 tgctcaaatg gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt
    10681 cgtgaacagc tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta
    10741 taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca
    10801 acacgcataa aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
    10861 aacacgtata aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa
    10921 aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc
    10981 cagcctctat tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca
    11041 gtgattcttc taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat
    11101 aatgttttcc tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca
    11161 ctactagttg ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag
    11221 cttctcgttc tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt
    11281 cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt
    11341 ttggttggat gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg
    11401 ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat
    11461 gtactggcac ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt
    11521 tgatcaagaa ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt
    11581 tttttatctg tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat
    11641 tttccaatgc ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga
    11701 gccgctgagc taattttata tccggtaaat atggctgttt catccgacga ttttggtaca
    11761 agacaaaaca gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc
    11821 ataataattc gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca
    11881 ctgtcggaga gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc
    11941 cggtttttgg cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca
    12001 atttttgctg gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc
    12061 aatctgttgc agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt
    12121 gcttatcgac tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac
    12181 tgcttgtgta ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc
    12241 attcttcaaa gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa
    12301 agctggctac taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg
    12361 ttccggcatt tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca
    12421 aagaagaagc aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt
    12481 gatcgggtga gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga
    12541 tcaaatgaag caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga
    12601 tgtacgctgg tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg
    12661 taaagtaagc ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat
    12721 tatcagggat tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc
    12781 gcccacctgc ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt
    12841 tgactacttg actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac
    12901 cgctgatttc atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac
    12961 caaatggatc aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt
    13021 aacatatggt tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat
    13081 agtttaaata acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact
    13141 acaatgttag aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa
    13201 atagaatcat ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt
    13261 tgatcactcc gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag
    13321 cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta
    13381 acgctggatt cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg
    13441 tgatggccgc aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa
    13501 tgatgatgcc gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca
    13561 ctgtcccaac tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca
    13621 tgcttaacgg ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc
    13681 gattatctcg agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc
    13741 cgactaaagc acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca
    13801 aagctggaat aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg
    13861 atggaagccg gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa
    13921 agatcaagcc accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta
    13981 agaccgtcca gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc
    14041 ttactccagt gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat
    14101 cgcctaataa aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa
    14161 tatttcttcg ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc
    14221 gtgatagcca ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga
    14281 tctttcagtg ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca
    14341 aagaaccagc agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta
    14401 ggatggactt ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga
    14461 cgaacaaatc cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag
    14521 atcccataaa tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa
    14581 gccgctgatt ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata
    14641 aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga
    14701 atgccaagac cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag
    14761 tctaatttaa atgcaaaccc gagaataaca atcaagacgc ctaaaaggct aagataattt
    14821 tccatatatt ttcctccata aaatgacaat atttttttaa ttataaagca aaacacataa
    14881 aaaatatagt tgttttctca cgaaatagac actattttta aatatttttc gttatttttt
    14941 tatcacaaaa attatcttac ttattgtaag gtaaagcttt ttttaattta ttgcccgata
    15001 atatatagaa ataaggtcta gttactgttt tttttaaatg attaataaat aacaacttct
    15061 ttctatgaaa ttgcaaagca ttcatcttct tttccgctaa cgatatatcc gtcagtgctt
    15121 ggtacacttt actaaaaagt atgagattgc cgatatctgg ataggtgtgc tatactacag
    15181 aaaaagatat acaatctgcc accgggtagg ataaagagga tgatcattcc attaggtctt
    15241 tgaagggatg attttcctct ttttgtgtat ttttttatag ttaaggagga atgtaagtat
    15301 ggcatggatg gaattaatca tagcaggaat tttggaagta ttctggtcaa cgatgatgaa
    15361 atggagtgat ggtttttcta aaatcaatta ttctttttac acagtgattg gcatgattgc
    15421 tagtttttac tttttgtcaa aggcaatcaa aagtttgccg atgagtctct cttatcctat
    15481 ctggacaggg attggcgcag ttggttcggt cataattggt gtggtcttgt ttcatgataa
    15541 gctgaatctt tccacttggt tctttgtggg gatgctactc atcagtatca ttggcatcaa
    15601 gattactagc ggccactaga cacttggaat aagtactgat cagaaaaaat aagccttttt
    15661 atttaatatt caattttttt atgaaaaaat aagaaataag tccaatgacc tagcgacttc
    15721 tccactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag
    15781 tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag
    15841 ttttagctta tcccctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac
    15901 aacgatcatg aaagctattt taggactgat ccattactca gatggaacaa tctgtattgg
    15961 tgaaaatgag atttctccct ctagtcataa agggttggaa caagtaggag cattgatcga
    16021 atacccgggt atctatccat tcttaacagg ctatgatcat ctgaagttat tctctgaaac
    16081 caacgatgta tctgctatcg acactatcgt gaatcagctc aagatggaaa agtacattca
    16141 caaaaaagcg aaatcctact cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt
    16201 actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc
    16261 aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat
    16321 ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca
    16381 aggaaagatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat
    16441 cgttatctct acaacaaatg atctagctgc tcgaacgatc ttgacagaag ctggatatac
    16501 tttggaagat cctgataaaa tcaagctttt aaaagaaaac gaagaattat taggaaccgt
    16561 gatccagcta ttgtctgcac aaaatatcca tgtgacagac gtccagcatc tgacgaatga
    16621 tttggaatct tctctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgaca
    16681 ttgcttgtaa aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct
    16741 gttctattag ttgttttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc
    16801 attgatccag ttgaaatgac tgctcagctt tttagtgcca catcttggat tgtctttatc
    16861 atgattgcag ctgcaagcac gatcatttcg atggaagcac aatatggtac attgaaaaat
    16921 ctcctttatc gaaagtattc tcgaggagaa atattagtaa gcaaatggat cacattagtt
    16981 atttattctg tttatttata ccttttagct attattgtca ctgtgttgat gaaactgatt
    17041 cttttccctt cgatttcctt cacggagaag gtttctactg gtcaaacact gatccaaagc
    17101 ttgttcactt atactttggg tagttatatc ggtttatggc tgattttaag tttagtgttg
    17161 atgattgctt gctttatcaa tagttccggc gcatccatct cagcaggaat cgtcttttat
    17221 tttgcttcgt caatcatatc tggtatcctt attgcactca tccaaaaatg ggagtggatc
    17281 aaatggaatc cgatcagtat gttgaatctg caaaaccaaa ttggaaacga agagatcatg
    17341 aagcaattga ctcatctttc tactaatcaa atgctattcg gtaatttagc ttacatcgtt
    17401 ttgttcttag cattaggata tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa
    17461 aaagaggctg tgaaagagac gttcagcctt attgccgatt ggctgtctct taaacaccgt
    17521 ttattctgaa aaaggagctg tgacaacatt tgtgtcacag ctcctttcct agtatgtttt
    17581 tgaataaacg acaggctcac aacctctctt tatacttctt ttatacttct atacgtgaag
    17641 catcccagtg gcaaataaaa ccattgcaat cactccgacc atcgtcacac ctataagtaa
    17701 aatcttatct aagtggatat gttgtacagg ctcgttttgc tccttacgtg ctttgaggta
    17761 aaaataaatg cctggcaaga aaagaacaag aacaagcatt acttcattaa aggctacgac
    17821 tagcatagcc catacttgaa aggcacttgc taaaagccct actaaaaatt ggacccattc
    17881 tttccgctga taagaaagtt tcatttggta caatcctaag aaaagccacg tgaagaaaat
    17941 agctgctgta ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata ataatgaaag
    18001 taaaaagaca ttcgtacata acgtcgtcaa tacgatagca taagttggtg ccccattttt
    18061 gttgagtttt ccccatgctt taggcaaaag tccgtcttca gccatgtttc ccattgtttc
    18121 aatcggtaag atcgtccatg agagccaaac accaactaaa gaaatgatca aaccgccgtt
    18181 gatcacgact gctccccatt taccaaccat catttcaaac acatagccca tagcaggctg
    18241 cgccttgatc gtttgcagct gattcatcgt cattgtccca tatggaagca tggaaaccaa
    18301 gacgtaaata ataatcaagc cgaatatccc tagaatcgtt gctttcccaa catctgattg
    18361 tttttttgct cgtgaaccta cgacagaggc tccttcaata ccaacaaata cccaaagcat
    18421 caccatgatg caactcaata cttgactgga aaacgggatc ggctgtccat tcgcattcaa
    18481 tataatgtcg ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac
    18541 tacaataaat aaaaataatg gcactaattt acagaatgtg acgatagcat tgacgaaact
    18601 agcattttcg actccccgat tcactagcca tgcatatccc cataaaaaga ctgtactcat
    18661 cacgatcgat gccagatttt gcccaccttc aaatactggg aagaaatatc ctaatgcgct
    18721 catcaacaat gttgcaaaag ctacgttgcc taaccagcat gtgagccagt aaccccaacc
    18781 gctgataaaa ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga tcccgccttt
    18841 taaatctgga cgttttttat tgatattatt gatcgacaag gagagcatca acattgctgt
    18901 tcctacgagt acccaactga aaatagctgg gccgccagcc gatccggaga tgtccgtcat
    18961 aataccaaat acgccacctc caatcgctga accaattact aaggcagcaa gtggaattaa
    19021 accaattcct tttttctgtt gctctgtatc catgtaagca tcctccaaaa taaaaccgct
    19081 tttatcaaaa agtataacaa acctttttta tttttactta attaataaaa actttaatag
    19141 aaatataatt ctaccggctt ttttctcatt atacaagcaa agcgcctagt ccctttccaa
    19201 attacaccac caaaaaactt aaaaaagatg ctatactaaa aggaaagaat tggaggatga
    19261 tccatgaaca ttttatatat atcgatttca ggaaatacgc gtgcctttgc caaacatcta
    19321 gcagaatatg ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt tactttaaaa
    19381 gaaatccatg aaaatagtga ttttacgaaa gaaacagaac cattttttac tttcgttcct
    19441 acttatttag acggcggaaa tggtttagat aacggtgata cagaaatctt gaccgaaacg
    19501 atgcgtgaat atctggaaca tgaagacaat cataaacttt gtcttggtgt agtcggtagc
    19561 ggaaataaaa acttcaataa tcagtattgt ctaacagcca aacaatatgc acaaacattt
    19621 ggctttcctt tcttggcaga ttatgaatta cggggaacac catctgatgt gacacgaatt
    19681 tatcagattt tagcagaaaa caataaataa taaaaaagaa gaagctgaaa aatagatttc
    19741 agtttcttct ttcgtttcta ttgtttctat catattaagc tttgacatcc atcacaggtg
    19801 cttttgcttt tgctttacct gttttttcca agacaaagtt ttttacacga tccatattgg
    19861 ttaccaagac gatcatggat gtttctttgc ctgctgcttt gatcgcttct aagtcagctt
    19921 ctgctacaag cgtatcaggt gtcacttgct gcccttctgt tactttcaca tcaaatggcg
    19981 taccgcctaa gtcaactgtg ttgattccca tatgaagcaa gatttctaaa ccatttggcg
    20041 ttgtgatccc aatagcgtgt ttcgttggga agacagagct gactacccca gtaactggag
    20101 aatagatatg tccgttttct ggttctacaa agtagccttc acccatcatc ttttgtgcaa
    20161 aaactggatc acttgcttta tctaaagaaa caactgttcc gtcagcaact gatgagagga
    20221 tagtcacact ttcagctgaa gtttgaggta ctactttttc aggcacttca tttactgtgg
    20281 ctgtttcagt cgctaattct tctttttcta aacggtcttt aggaataccg aagaagaatg
    20341 tagcaacgaa gccaccagca taagctgcca ataatccaag gatgtagcct aaccatttac
    20401 cgtcagagat caatgggatc aaagcggctc ctgaaggtcc aatagcgata gccccgacat
    20461 ttccgatcat tccgatcact gccccgccga tacctccacc gatacaagca gtaataaatg
    20521 ggcgtcctag aggtaatgtt actccgtaaa tcaatggttc gccgattcct aggatcccga
    20581 ctggtaaagc tcctttgatc atttctgtta attctttatc ttttttgcag cgaatccata
    20641 atgccaatgc tgcacccact tgtcctgcac cagccatcgc aagaattgga agcaatagcg
    20701 tacgaccagt ttcatcaatc atttgcacat ggattggagt caaaatttga tgcagtccga
    20761 acataaccat cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc
    20821 caagtaccca gttgatccca ccgaccatgc catcagaaat gaaacctgct aaaggcatga
    20881 tgaagaaaat ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta acgatgatat
    20941 ctaccgcatc aggtactact ttatgcagtt ttttctcaac gattgacaat agccaaacag
    21001 cgaagatgac acctaggatc ccaccttgtc cagctgcaag agcttcacca gtaaataagt
    21061 ttttcagtgg tgcttccgga ttcatccctg tcagcatcac aacggccccg ataactccac
    21121 caagagttgg tgttgcacca aaaacttgtg cagcattgat acctgtatag ataactaggt
    21181 agctaaacat cccgtttttc aagatgttca tcacatcgat atattgttgc catgttgctg
    21241 catcaagtgt tccggctgtg actaagtttg ctaaaactgc ggcaatccct gccacgatcc
    21301 cagtaccgac aaatgcagga atcattggca caaagatatt agaaatgtct tttaataccg
    21361 atttgatttt agatggtttt tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt
    21421 cgtttacacg gtcttttcca gatgctgatg aatttgctgt agctgtactt acagaagaaa
    21481 gtttttctcc taatttcacg cctgccaaat caaccatttg ctgtgctact ttgttcactt
    21541 tcccaggtcc gatgatgact tgaagctgtt catcttctac tacaccaagc actcctggaa
    21601 tcgcttttaa ttcagagaca tttacaagat catcattgat gatcttcata cgcacacgag
    21661 tcatgcagtg gacaatgctt tctacattgc ccatgccgcc tgccccttta taaatttgtt
    21721 ttgccagttc ttccaaagtc agttcgtttt ttgccatggt cttttccccc tcataaggtc
    21781 ccttttacaa atccgtttgc ttcagctaat tttgcttgag cttcctcttt atcactattt
    21841 gtcaaaatca tgacgatagc cagcttaaca ttttgatctg cttcttcaaa gtacttttca
    21901 gctgtttcgt aatcagcttc tgtcgcttgc atgatgattc gttttgagcg ttcgattagt
    21961 ttttcatttg ttggtttgac atcgaccatc aagttattga atactttacc tataccaatc
    22021 atactgatag tagaaatcat attcaagata agtttttgtg ctgttcctga cttcaatcga
    22081 gtagagcctg tcaaaaattc tggacctgca tcgacttcga ttgggaaatc cgcgtgttta
    22141 ctgatttcgg cttgtttatt gcaagaaatc gttcctgtga cagcaccgat cgattttgcg
    22201 taatctaatc caccgatcac atatggtgta cgaccgctag ctgcgatacc aataactata
    22261 tcattttttg tcaaattcag cttgattaga tcttcttgcc ctagctcata agaatcttcg
    22321 gccccttcga cagctacagt cattgcttct tctccaccag caattagacc tatgaccatt
    22381 tccggttcta caccgaatgt cggtacgcat tcagcagcat ctaacacacc tagtcttcca
    22441 cttgttccgg ctcccatata aatcagacgt ccaccttttt taaaggactc aatcgtgtta
    22501 gcaatcactg gttcgatact tgtcagttct ttttcaaccg cagcggccac tttctgatct
    22561 tctttgttca ttagttgaac ggcttgagaa atactcatct catctaatcc aaaagtttct
    22621 tggtttctgc gttccgttgt taggttctct aaattgattt tgctcatttt tatcttcctt
    22681 cttttcttat aaattgatag ttcataccag ctttgatttg gttgatcaat ggtagctctt
    22741 ctttgataac tttagctact acattcactt tttcatccgc tggcaaatca tatttggtta
    22801 attggatctc acccatatag cgcaaatact tttcgttatc taaagtaata gaccctttca
    22861 tacgcgtagc tgtatttctt gctgggatag gcggaatctc tcgaaaacgt gcatctacgc
    22921 ttcgtatgac atctcttgca tcgtcttgcc gattcgtatg cttccctaat atatattcgt
    22981 aaaattcttc atctacaggt tccgtatgga gcaagactgt ctgttctttt tggaaagaag
    23041 caaattgctc ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca atatccgtgt
    23101 tacttgctaa tagatctaga gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag
    23161 ttggcaatcc ttgatatagt gggcctctca actctgtatc tcctggtaca aaaccttgga
    23221 caaggagacc ttgaattttg agccattgat tttttttctg ataccattct ttgtccagcc
    23281 ctgtttcggg acgtggataa taattatgcc aagcttcaat ctgtgagaaa tcagcttctg
    23341 cttctttcaa ttcatcgata tctttaggcg tgatcgtgct agcattcaaa ctgattttca
    23401 attgatgtga ccagtcggca atctgctgat tggaaatatg ataatccatt cttaaacctg
    23461 ttacaccaat tgctttcaaa ggcgttagat cttctaatga aaaacccgct cgttccaatg
    23521 cctctcctga aatatcgacc atcaattcta gtttattctt tttcgcttcg cttcctagtt
    23581 cgatcaatcg cttttgatat gccgaaacat catcttctgg tatatgcatg gaagtgaaaa
    23641 taccgacaaa gccattggat gccatttggc ggatatactg tttcttctct tctgacaaag
    23701 aatcgttcat aaatattgaa aagccaaaca tattatccct ctttttctgt atccgtcttc
    23761 atttctatga ctagagtata tatctaaaga aacaaaattt caataatttt attaaaataa
    23821 ttaaatttca tttctaaagg ttgagtttat attatcaaaa gaattgtcat aacctaaaaa
    23881 aggcactgct aagcaaaagc agtgccttga aatattttat ttaaataaca caaaaacata
    23941 attactcata agaaaccaat tctgtattat ctaccatgaa tgtaaagaac ccctcattag
    24001 gcatcggatg aaaataagca ttgatcactc ttatcaaggc attatggcaa actaacagat
    24061 agacattttc ttcgtcttcg atgcattctt tcaataaagg aacgatccgt gcatacacat
    24121 ctaagactga ctccccattt gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt
    24181 tctcatcttt gcttggcagt ccatcataat ccccaaaatc catttctacc aatcgttcat
    24241 caacggtcag cggcaagctg agacgatcag caactgcttg tgctgtatct cttgctcgtt
    24301 gaagagggga atggatgatt ttcgtgattg gaacaggcaa atctactacc ttttctgcta
    24361 gtttctgtgc ctgcatctgt cctttttcag tcagcggcac atctgcccgt ccgcaaacta
    24421 atccttgtgc gttccaagtt gtttctccgt gtctcgttac ataaagcatg tgattctctc
    24481 cttcgttggc aaagttcccc ttaaccatac cttgttgttc ttgagattac aagggttaga
    24541 ttctctcaag agaaataatc tcagtagata ctttatccag gaaggtttga tcatgttcca
    24601 ctagtaacat ggcaggtttc acgttcaaga tcagttgttc taattgttct tgattgaaga
    24661 catccaaata attcaatggt tcatcccatg tatatagttc agctggctgt gataaagatt
    24721 tagccaattc cacttttttc cgttggccca tactcatctg ctcgatcttg ttatgaaaaa
    24781 catctctttc catcccaagc tttcggaggt tgttcaaaaa tgcttggtag tctacttggt
    24841 ttttctctgc aaattccgcc aacgttcctc gattgtcttc ataattttgg cttgcataac
    24901 taatgctcag atgttttggc tggtattttt ctcctataac cttgccgttg aatgccccca
    24961 gaagataatg gataatggat gactttcctg caccgttagg acctgaaatc gccacccgct
    25021 gatgaggctc gattgtaaaa tgaattggct cgaataacag attttcatac cctaattgaa
    25081 gatcttctac gcttaaaagt cgcttatggt gagacgcttg gctattcatc gtcaaagaat
    25141 cgatatattc gatatctttt aatagttttt ctttctcgct gatctgggtc tccatccgat
    25201 tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc agcacccact gctcctttat
    25261 tcactcgtct agattcagta tcgatgaatc cgacttgttt ctttgtttta tctccttctc
    25321 gggaacgaga ccattcggct ttttcagctg ctgttttctt tagcctactg acttcttttt
    25381 tcaatttttc attttgagcc atttcaaatt catcacgaag ttttttctgt tcttcataga
    25441 tagagaaatt cccttgataa agttccagtt gacttttttc gattgctaaa acatggtcca
    25501 ctacttcatc gataaatccc cggtcatggc tgaccacgat gaagccttgt tttttctttt
    25561 tcaaataagc cgctacttgt tttctaccag agatatccaa atgattcgtt ggttcatcga
    25621 ttaacgggaa atgagtgtca tccacaaata aaagtgccag taggactttt gtcttctctc
    25681 cccccgatag tgtgctgaat tctctccata agatttcagg atctgtttgc atcaattgga
    25741 gctctctttc gatttcccat atctcaaaat ccgtaatatc atttaacacg taataggtta
    25801 aacgttcttt atcttttgtc tgttgcggga aataggcaaa ttcttgctga tggattactt
    25861 gcccttgata aggtagtttg ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt
    25921 ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt tagatttgct tgttcaaata
    25981 ataatgtgcc ttggctgtcg tagccgaatg tcagattttt tatttcgatt ttagacattt
    26041 tccatcacct cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag aggatgcaag
    26101 ggttggatac acacaaaaag acccatacat cgaaaaaacc aagccaatcc cgaaaatctg
    26161 cacactgctt ccttgtctcc acaaggtaca gtctgaatgc agctttcatc aatcgactta
    26221 atttttcaat gcacggatcc ctcactttgt cctatatttg aaattacgat agcataaaga
    26281 aaatgatttt gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa
    26341 gatttttgtt ttttctaatg tttgcctgtg ttccattgct tcttctaaac ctattggacc
    26401 caaatgaata cttaagacaa ttgggagaaa atcagaaagt ccttctagct cttggtctct
    26461 acttccgcaa atagcgatta ttggttttcg atattttttt gcaagcactg ctacaccgta
    26521 tggaactttt ccgtggatcg tttgctgatc gatccgccct tctcctgtaa tgatgacatc
    26581 tgcttgttcg atcttttttt ccaatccagt gatttggctg actaaatcga aaccagaaac
    26641 catcttcgct cccaacaaaa aaagggctcc accaataccg cctgctgctc cacttcctgg
    26701 aatatcatct aaatccacct cccagaaatc ttttatttgc tgagcaatct ttcctgcttg
    26761 tttatctaag aaagctagcg tcgtatctat tcctcctttt tgtcttccaa attgccaagc
    26821 agctccttgt tgacctgtat atggattggt aacatctgca gcgataatca attttacttt
    26881 agaaacaaga acgtttcttc gatcaaaatg aagtttttta gtagataata aaggatttcc
    26941 ttctgtgtac ccttctaaag aagcacccaa ggattggagt aatcctagac caccatcgct
    27001 acacccgctt cctcctaaag tgacaataat ttgttctact tcttgaagca gtgcatcttt
    27061 aataagttct cctaaggcat aactggaagc aagtcggata gtttgatccg ttggtttatc
    27121 aagtagatct aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca
    27181 agttgtcaat aaatagctga tttttcgctg acggaataag agatccatcc catcaatggt
    27241 acgccatgtt cccctgagcg cgtaatagat tgcttctgca gtattttctc cgccatctgc
    27301 aatcggcaca ttgatttttc ttcctttacg atcttcccag ccatttagaa ctgcctgatt
    27361 tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg
    27421 tatccctcac tttcttctta agtaagacac tttttttcat ttttttcaaa caaaacaatg
    27481 ctattttctt ccatttttct atatactaaa agggaactca ttgaataaag gagagaaaaa
    27541 acatgaaaat cgcaatagct ggggccggag caatgggcag ccgaatcgga ttgatgctac
    27601 atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt gaagccattc
    27661 gtacaaatgg gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct
    27721 attcaccaga agaaatcatc gaaagcaacg aacacgtgga cttgatcgta gcattaacaa
    27781 aagccaatca attagatgat atgttttgtt cgatccaatc cattattact gacaatactt
    27841 atgttttgtg tttactaaat ggattaggtc atgaagatgt actggaaaaa tatgtgccaa
    27901 agaaaaatat cttatttggg atcacgatgt ggacagctgg tctagctggt cccggaaaag
    27961 tcacattgtt aggtgatgga gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat
    28021 ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt aaatccgatc tatagccata
    28081 acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca
    28141 cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag agcatggttc
    28201 gaacaatcgt atctgaattt gcttctatcg cagcaaaaga aggaatcata ctagatcaag
    28261 aagaagtgta ccaacatatt gcttctactt atgatcctga caacatcggg ttgcattacc
    28321 catctatgta ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg
    28381 gtgccatttg gcgaaaagga caaaaatatg atattgctac accatattgt gcctttttga
    28441 cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag
    28501 aatcagctga atcagcagct ggttctcttt ttttaagccc aataaaagat caaaaagaaa
    28561 acacaactct catgatgcca taagaattgt gcttgtttgt ttcagtacat aatctcttca
    28621 aaatgtaggt tttttatttg ttttcaacag aggagaggtc atattcgtat tgctcataaa
    28681 tagtggaatc gttagtgata aatcgttttg cccaattttc tgcatcataa aattccaata
    28741 gctctgatag gattggctta aatggtgtat gcagaaattc cggtaattca tctaaatcac
    28801 gttctcgaag caaaaaaata ttttcaggac gataaaaatg ataatgagca atcgcttgat
    28861 tatccgtcac tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt
    28921 gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt ttcaggtact
    28981 cttcataagt aagatatcca ggttgttcat tccctcgatg tggaagaatg aaatagttgg
    29041 tcgtaacatc tctttttata aattcctgct tatagctttc tgctcgttct tttctcccat
    29101 tattcgttgc accaaaaaat agatccgtct ttggttcgct agaagaaaaa ttcatatgtt
    29161 tcgaataaaa tgaactatta tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat
    29221 gataaatctc atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg
    29281 ctttgaagtg attccaaaaa aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc
    29341 gagccatctc catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact
    29401 gttgtctagc tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta
    29461 aagagcggat ggaaggcaaa aaataaagtg atttattttt tgcaactagc agtacgtcat
    29521 ttttgttcat cttttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa
    29581 atagtaaaaa gccagtaaaa cgtttcctat atttgtatga aaatttaaca tttgtatgaa
    29641 aatatgatga aataaatcat tcttctatta aaattcgcct aaaagttttc tgaatagttg
    29701 taattgtctg aaaatttgtt atcattagtc attgttaaat attgctattt attcttaata
    29761 taaattttga ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa
    29821 aacaacttag ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc
    29881 tatttaaagg aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt
    29941 tgattggtgg gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg
    30001 gtggcgacac gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg
    30061 ctgattttac tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg
    30121 ttgcagctgc ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt
    30181 ttatgttggc tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa
    30241 cagaatattg gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta
    30301 cttatcttgt cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga
    30361 ctgaccatgg tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag
    30421 ttatctattc ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc
    30481 ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca
    30541 tcattccttt gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg
    30601 tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca
    30661 attttgtgat tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa
    30721 gaattttgtt cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa
    30781 acaagcatca agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg
    30841 tagctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt
    30901 atacagtatt attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa
    30961 aaggaacatt atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt
    31021 tccttggtat cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc
    31081 tgttcattta tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa
    31141 atcgatagaa aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt
    31201 gtgacaaaag tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta
    31261 aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga
    31321 tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg
    31381 ttgatgatcg gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg
    31441 ctcgatcata tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc
    31501 atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt
    31561 atgatcttag ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa
    31621 actactgtca acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc
    31681 ctggcaagta aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc
    31741 attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga
    31801 ccattatccg tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa
    31861 gaagaagcta cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa
    31921 gtaataagaa cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta
    31981 ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc
    32041 tgagtattag agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa
    32101 taccgagggt cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt
    32161 tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta
    32221 ataccgagaa actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct
    32281 tgtcacaatc cctttttatg tttttattgt tctaattgcg ctgtcttgct tactgcttct
    32341 tgcggtttca caaatttttt tgagattccc caacctgtga aggcactgac catcgaaaac
    32401 acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg aataccaaat
    32461 gtacttgcta cgaaacttcc ggcaattccc caaggcacta agtagttgat tactgttcca
    32521 ccatcttcta gcgttctacc taatacagtt ggatcaaatc ctgctttttc ataagtttct
    32581 ttaaatgcat taccaggcag aataacagat aggaattgtt caccaataaa aatatttact
    32641 ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat
    32701 actcgttcca tcacggttga gatcaagcca aattcgatca acaatccacc taatgacaaa
    32761 gttagaataa tcaatgacac agtaggcatc atactttcga ttcctccgcg gctaagcagt
    32821 gaatcgattt gttggtttcc agtatttgaa acaaaccctg attcgatgac cccagccaac
    32881 ttcgttaagg aagtttgtgg gttttgaata aaaatcattc ctactgctac agcgatattc
    32941 aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac acagtaacat taaaaataaa
    33001 ggaatcagtg accagattga aatagtaaag tttgcttcta gaacatcagt cacttcacga
    33061 atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa
    33121 acaacaaatg ccgggacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca
    33181 tcaacaatag ctgctgctag atttgttgat tcggataatg gagacatctt atctccaaaa
    33241 acggcacctg aaacaatagc tccaacaacc agcgctggat ttaagcctag tgttgtccca
    33301 ataccgaaaa atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta
    33361 cctacgattg cacacacaac aaatacagaa gggacaaacc actggacact gatcaatttg
    33421 aacccaatga ccattagagt tggaatgatc cctgcttgta tccatacagc aatcaatgaa
    33481 ccgactaaaa taaaaataaa gattgggatg attcctggtt tgatgccatt gatgattcct
    33541 tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat acaacatgac cattccgatc
    33601 gctgctaaga tcgtgatatt aggtgagacc tttaatccga caacgccaat cgcaatgcct
    33661 aaaacaataa ccattaatac aagtgttgct tctaaaaaac ttggtgttcc tttttttccc
    33721 atgataatct ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta
    33781 ctccgccgca attcatccgc gattcctcct ttataaaaat aaaaagacct gttttaaact
    33841 aaggacgaat gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa
    33901 acttttattc aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac
    33961 agcgaccacc gactttctga acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt
    34021 acgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt
    34081 ctgaaaatta tttttataac tttcacacac aagtttattt tttactagaa agtagcgccc
    34141 tcttgttttt ttcttgctac tatttcttga tgttcttgct tgattctttc taatagcttt
    34201 ggctctttca acagatcaag agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa
    34261 gcaaggcctt tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat
    34321 gtctcagaga ttgctaccgt aggctggatt gttggtatga cttggctgac atttcctaca
    34381 tcagaagaac cgagacttgt tctttgttcc gtttctattt cttcatctgg aacacctgct
    34441 tcttttgcat gctggaagaa taagtcatca aaagaaggag taacgatgac atcatcaact
    34501 gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg
    34561 atattttcca ctttttcata tactttatct aatgtttgcc gatttcctgc acgtaaatag
    34621 aaacgtccag cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc
    34681 ccatggatat tgacatcttt tggcaattgc aagcgtagag cgttgattcc attaaacact
    34741 tggatcaatg cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgggaagcg
    34801 acaccgtaaa atttgatgtc gactggatca ttcgctaaag attctgtcgt tttcccataa
    34861 cgaaaagctg gatggacgca taacgctaca tcgacatcct caaaaaaacc ttctcgaaca
    34921 aagctgcctt tagctgatcc gttctcgccc ccttcttccc ctggggtgcc atatacacgg
    34981 atttctccac cagtttcatc aatgaattgc ttcactatgc tggcggctaa tacggagtaa
    35041 gtaccaaaca aattatgccc gcatgcatga ccaatacctg gtaaagcatc gaattctgcc
    35101 aaaaaggcaa ttgtgggacc gggtttattg gaaatataac gtgcatcaaa ggctgttcgg
    35161 tgtccagcta cttctttttc tacccaaaac ctttcttttt ctaattgttt aatcaacgct
    35221 gctgaggaat aaaactcata gttacttact tcaggatgtt cgtggatatc tagagcaagt
    35281 gcttgatatt ctggtaaacg atattggatt agatccttca attggtttgt tgagattgtt
    35341 gttgtcataa aaaattcctc ctatgctagt catttttctt attttctccc gaatgtttcc
    35401 catgcaggca cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt
    35461 tgataagcgt ctactatttt ttgatacgtt ttattgtttt catcttcttt tcttgcaacg
    35521 atgatattca cataaggacg tgcatggtca tccactggtt ctaagaagat ggcatcagaa
    35581 gcaggattaa atccagcatc tacagctaca ccgctattga tcactgaaat gtctacatct
    35641 gacaaagcac gtgctgtttg ggaagcatct aattcagaaa tgtctagatt ttttctattc
    35701 tcggtaatat cagaaacagt gggcgcttgt ccttttgcct tatccacttt gatcaatcca
    35761 gcagtttgca ataacagaag agctctgccg ccatttgtaa catcattagg gattgctact
    35821 tttgctccgt ctttgatatc agctacatcc ttcaaagtgt tagaataaat tcccagcggt
    35881 gcattgacag tatttccgat cgatactaaa tcagttccgt gttctttatt gtaattatct
    35941 aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc catctgctaa agccacattt
    36001 ggttgattgt aatctgtaaa tttcacgtat tccaaattaa tgttctcctt tttcaaacgg
    36061 tctttcacgt tgtcccatac atctgtatct tctccaataa tccctagttt tacgatagtt
    36121 tcttttttgt cagttgtatc tgcttttcct tggctacacc ctgccaacca aataattgct
    36181 acaaatgtaa tgactacaaa acgaataatt tttttcattt tttctcctcc actttcaatg
    36241 tgtaattttt ttaatcagtt gattactgat aaattgactt aaaaaaacta agattagaat
    36301 caataatgta gccacaatcg ttacatctgt ttggaatcga ttataccctc tcgatatagc
    36361 aagattccct aatccgcctg cacctactgc tccagccatg gtagtcagtc cgatcacgtt
    36421 gatgatagtc aacgccgaga cccgaatgat accaggcaaa ccttctaaca aatacacacg
    36481 taaaatgata ccgattggac tggttcccat cgcttctgcc gcttcgatca ccccaggatc
    36541 aacttctagc agtgcgttct cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa
    36601 tggcactaaa gctgctttcg caccaatcgt cgttccaact aagaaacgag tagtcaatgc
    36661 taaaagagaa agcaagataa tgaaagggat ggaacgaatg atattgatca gcttatcaag
    36721 tatttcaaat agcagacggt tagctagtaa tccattaggg cgtgtcacta ctaacgtcac
    36781 acctaagatg attccaatca tcccgccaat gaatgctgtc caaaaagaca tatatagtgt
    36841 ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa acattgggca aatatgtttt
    36901 aaagaaatca gtcattttat cttcctcctt cgatcacttt gatacgacta tctaaatcgt
    36961 ttttttgtaa aatatttatt tttacaccag atttgatcaa ataatccaat gcttttttcc
    37021 gctgtttggt ttctccagct aaagtaacga tcaaactccc taacggcgtt tcttgtaata
    37081 actctacatt gccatataaa atattcgctg ttacttgata tttgctgtac agatgagcga
    37141 ttagaggttc gttcgtctga tttccaatat aacttaattc caccagccat ttattggcga
    37201 tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca agatgagtag
    37261 ctgttcgaat aaaatctttg gtcaattctt cttcaggatt gctgaagatc tggatgctgc
    37321 tacctttttc aacgattctt ccatcttcca taaccgctac cttgttgcag atttctttga
    37381 caacttgcat ctcatgagtg atcaggacga ttgttaaacc taactggcga ttcaattttt
    37441 ttaacaatgc aaggatctga agggtcgttt tcggatcaag tgcactggtt gcttcatcac
    37501 ataataatac cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc
    37561 cccccgacag ttgtttagga taagcagatg ctttttcttc caaaccgaca agttccagta
    37621 gttcattcac tttttgacga cgttcctgtt tcgattttcc agaatacttt aaagaaaaat
    37681 caacgttgtc aaaaatagtt cggctttcca ttagattgaa atgctgaaag atcataccga
    37741 tggcctttct ttcttttcgc aattctttgg gagagagctg actcagattc gtctgattga
    37801 caatgacttc tcctgctgtt ggtttctgca gcaaattcat cacacgaact aacgtacttt
    37861 tccctgcacc tgagtaaccg acgattccat aaacatcgcc tttttcaatc gttaaatcta
    37921 cagaatctac tgcctgtatc tctttttctt tttgtttgaa acatacagaa acatctcgca
    37981 attcaatcat taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc
    38041 attttcttga ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac
    38101 aactcctctt caagtacgat catactctag caagatatcg gtcgcagccg tcatcgcctt
    38161 acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct
    38221 tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa
    38281 ataatattca aaaaaaaagt ccttttacta acataaacta tgtcagtaaa aggacgacgt
    38341 atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag
    38401 agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta
    38461 ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct
    38521 gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa
    38581 tctacatgag gaaaactgaa aagtcaagta cgaaattcaa attatttttt ctatatttat
    38641 ttttctgttt ttttatttaa catttcaata acagttcgaa gatcttttcc ttccgcaaga
    38701 agtttcaatt tttccttttc ttcgacggta taatttcgga caaaactgga atcaggtatc
    38761 aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga atcttccact
    38821 gcaaaggctg aactagcttc taatcctgtt cgttccaaag ctgtcagata aggaatcggt
    38881 gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg tgtccaagta atcagctaat
    38941 ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt
    39001 ttcatatgac gttcttttaa gtctgccaat atttcttttg catatggttt taagtggatg
    39061 gctccgcttg tcagctgttg ataaaaatat tcttcacgct tcgctcggac ttcctgtact
    39121 ttttcagggc tacctgctgc tttcactaaa atatcaaaac tttgtttcca gttttttccg
    39181 ccccaagcgt caacgatttc ttttgggata tggatatcaa attgctgaaa tccccaaagc
    39241 cagccatcac gatacgtttt ttctgtgtca actaacaaac catctaaatc aaatattgca
    39301 cctttcatct atacattaat cctttccaaa acaatacaca tagtttagca cgactggtcc
    39361 aaaaagaaca gaagcaaaga accagccttc tcgtttataa gagttcttgg aatcgacgga
    39421 tatagatttt cttcacagct gtcacaagcg ccagataagc gatgactgta gcaaataacc
    39481 ataaccagta attgccagga agtggcatca agcctaattc agcaccaaaa cttgtaaaag
    39541 gaaggacaga accgacaata attcctaatg tagtgataac aaacatggca aatgttgcgt
    39601 tgctttggat aaatggtact ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc
    39661 atagtgattc gacaaaccat cctgcatgga ataatgcgat gaaaatcact cgctgtgaac
    39721 tatccaatgt atggaaactt ccgcccacta cagcaggaca aataacaaaa tacatcaaca
    39781 agtaagtagt aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg aattccccaa
    39841 tactagatgc attccatttt tttggctcgt ccaaatattc tttatccatc cgatcccatg
    39901 gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa caaaagttgt aaaggcagca
    39961 ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga aaacatattg ccaaaattag
    40021 aacttgctgt agccttgata tatttcatga tattcccaaa agtttctcgt ccagaaagaa
    40081 ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca gattctttcg
    40141 caatatccac agctgtatct acagaaatac cgatatcagc cactttcatt gcaggagcgt
    40201 cattgatacc atctcctaaa aatccgacag tatgaccatt tttacgtaaa gctgtcgtca
    40261 aacgtgcttt ttgctgagga ttcaatttta cgaatacttc atggtcttca gctacttgtg
    40321 ctaattcatg atcactcatt tccgagattt tctcaccggt aaccaattca ttgatctcta
    40381 gacctacttg acggcacact gaacgtgtaa ctaattcgtt gtccccagtc agaactttta
    40441 cagctacacc atgttctttc agagctttca acgcttgttt cgttgtttct tttggtggat
    40501 caaggaaagc aagataacca atcaaaacca tgtctgactc atctttgact gaaaattctc
    40561 caacgacgct tggattggtc ttttgtgcga caccaatcac tcgtaagcca tcttcattaa
    40621 gtgactgtac ttttgcaagt acggcttctt ttcgttcttt tgttaatggt gtaacagtac
    40681 cgccaagatc aataaattta gaaatgttca gcatttcttc aatcgcgcct tttgtgatca
    40741 tttgtgtttt accagctgga tcagcaacca caacactcat acgacgacgt tcaaaatcaa
    40801 aaggaatttc atccactttt tgataattga tcttatctgt atctaaagtt tctgttgctt
    40861 catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag ctatttagat
    40921 acgcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa
    40981 tgattttatc ttgtgtcaat gtccccgttt tatccgtaca taacacatcg attgcaccaa
    41041 agttctggat ggcattcaaa tttttgatca ctgttccttt tttggccatc acacttgctc
    41101 ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggtgttaga ccaactgcta
    41161 cagatagtcc aaaaagaaaa gcttctagcc aatccccttt tgtaagccca ttgatgacga
    41221 taactgctgg agccatcaat gacataaatt tgatcagtag catggatgtt ttatggatgc
    41281 ctaattcaaa gcttgattga attggttttt cagatagcgt ttctgctaca tgaccaaata
    41341 gtgtttcatt tccaacagaa acaatgattc cttccgcact tccactgatg acattacttc
    41401 ccataaagac aagattttcg taacttgttt cgctttgctg atcgtcttca catactttcg
    41461 catgcttttc tactggatag ctttcccctg tcaatgctgc ttgggaaatg aataaatcct
    41521 ttgcggacaa taaacgcata tccgctggta tcatatcccc tgctgaaagc cgaacaagat
    41581 ctccacagac gatttcttcc ataggaatct ctgtgtactt tccattacgt tttacagctg
    41641 ccgttacttt cacaagtgat ttcagttttt cagccgcttg attggaacgt acggactgaa
    41701 tcaacgtcat tgtcccgctg acgataacca tagtgaagat aatggctaca ccgaaaagat
    41761 ctttatctcc aggagctgct aatacatatt ccgtgatgaa tgaaataatt cctaatgcga
    41821 tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg aatggtgttt
    41881 ttttgccata tgaaatcgca ttttcaccgt aattttctct tgaacgttcg atttgttctt
    41941 ctgtcaatcc atttgctgat gcatggaata gttcaaataa ttcttgtttc gtttttccag
    42001 caaaagctgc atagattttt tgtgtatctt tctttttttc catctcaatc tctcctttgt
    42061 gtccaaaaga cagctgttct cgctgctttt ggtctttagc tattcatcgg atcgatagag
    42121 aaaaaactga aaggggctga gaacagatag ataatagccc tggcctttcc tctagatcct
    42181 tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc
    42241 atgacttcca atcatttttc gaatagttat ttttccaatt agaccacctc ctttttattt
    42301 ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt
    42361 tgaaaaagca tattaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa
    42421 cgtaaaaagc agatacctgc ttttagctac cttatgaaaa gccttatctc gacaattcct
    42481 gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc
    42541 tcacctaaca aatcttattc ggttttcctt cgttaatata acaactaaaa aagaggatgt
    42601 caaatcaatt ttccatgttt ttcatacttt ttttaaaata cacacgttat attcctgtct
    42661 cacgcggttt tatttgtctc attttccacc tcccgctata atagaaatat agtaggaaac
    42721 gaggtgctgt tatggaaaga acacttgtta ttatcaagcc agacggcgtg agacgccatt
    42781 tagtcggaag catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaagt
    42841 ttgaaactat gactcctgaa ttagcaaaag aacattatca gcacttgact gagcgcagtt
    42901 tttttgatga actgatcgat tacatgactt ccggtcctgt cgtttatctt gtgctggtcg
    42961 gcgaggaagt gatcgatatc gtccgtaaga tggttggagc aacaaaagct gccgatgctg
    43021 ttccaggtac gatccgagga gactacgcgc tgccggggac tgaaaacatc attcacgctt
    43081 ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa
    43141 acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta aactgctggt ctagttgctg
    43201 ggttcagcaa tggccagcag ttttttctct aaggacctcc gttgattctt tcctaacata
    43261 tattcgagca ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc taaagcagca
    43321 gaaatcaatc tttgataata atctgccatc aatgtttcat agatcactgt acttctttct
    43381 ttatgtgcta agctccgttt catatcttca ttcacactac gtgccttttt caaccgacca
    43441 ttttctatat aaaacgaagt caggttcatt aaaaaagtaa agagatactg ctcatgattg
    43501 ttcaactgtg ctaatcgtcg cattcttttt ttgattttca aataattgtt ctcaatgaac
    43561 cgatcactga atatccaagg aaaatcagct agaatagata gttcaaattg accatagtct
    43621 ggtatctgca tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct
    43681 gtctcagata cttctgtttt ttcgaccatg tgaagattca aaaccaaata atacagatct
    43741 tctgttttct taaaatgcct acggttattg ctgatctctt tataaggatc aaagctgtct
    43801 tcttgtaccc gatctagcat tcgtcttttt ttctttaact ttattggttc atccagctgg
    43861 tttcgcatcc attcatattc tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat
    43921 gttacaaatg acattttttt cactgaattt gtctcaatcc cgactagaga agtcttaaaa
    43981 cagattcctt ccgctaattg ggactgtgtc aacttcttgt tgactcgaag tttcttgatc
    44041 aattcaccat catacataat agttctcctt tttaatttaa atttttccac attcaataaa
    44101 tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa
    44161 ataccaacct tcaagcatta gaatgttgtc taactactga aggttggttt gattgtttat
    44221 tgattatttt tttgctgttc aacgtctgtt cttgttactg gatataagtt ggttgttgtt
    44281 ttattaccat tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt ttcgattgct
    44341 tcattacgtt ccgcttcatt tttatagtca tattttttcg gatcaatcgt ctcaaatccg
    44401 tttggcacat aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca
    44461 gtcttgactt tttcctgatc tttggagatt tcctcatcga ttcctgctgc tttcgtatcg
    44521 atgatttctc ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggtgtc
    44581 acaaagttcc catttcgaaa agcaactact tgatcatgct gagaagataa taagtctgta
    44641 ccaaaatgaa gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca
    44701 tcgatttctc caccgtattc atgattgact gtccctttag tataaccagg catatggatc
    44761 atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta
    44821 ccaagagcgc ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa
    44881 atcacaaaga ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc
    44941 aatgattgat ccagataatg tgccgttcga acataattat ctactgtgct attgccagta
    45001 ttcaaagatg gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg
    45061 gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc agattctctg
    45121 aataaatctt tatctaatat cccataacct aacgtttcgt cggattcatc aaaataagat
    45181 cgatcaaaga aattgtcata acctaagttt ttatatacat gatctctatt ccaaaaacta
    45241 gctacattcc catggaaaac agcgcttgta tagccttgct tttgtccaag gatcgctggt
    45301 gcggcttgaa agacattgtc tgacccaagt tctgtgaata atgagccttg aggtaaacca
    45361 aatgtgccag tttccaacat attttcagca tcacttgtct ttccctgtcc tacttcatga
    45421 aagaaattgt caaagctgat cgttgcttga ttctggaaaa tactatttaa aaacggtgtg
    45481 acttcttgtc cgtctacctt catattaatc aaaaactgct ggaaactttc taaatggaga
    45541 acgatcacat ttttcccttt agcgatccca aatgtttcgg gatttggctc tgcataatgt
    45601 ttctttgtat aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg
    45661 ctagtcattc ctgttttgat tccgtcataa atagtatacg catcgattcc tagatatttg
    45721 acaatatatg aacggtcaaa tgttcgctgc agcaattgtg gacggttggc ttcgctcagc
    45781 gctagatttc ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc catcggtttg
    45841 ccgacagggt tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg
    45901 aacacagcga tatccagcca gtagatgaca tcatggggct tcatcaaaga aaaggagctt
    45961 cctgacaatc cttgcgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct
    46021 gtgaattccc gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg
    46081 atcaaaagaa tagctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac
    46141 aataaggtag tcgcaattgg gttgatccac aagataaagt actggatcgt accttccaca
    46201 cctaatgaaa aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct
    46261 gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact
    46321 tctctctatt cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt
    46381 tgttatctta agattttttt attctttcta aagattattc cgtccacttt tcacagcata
    46441 aaaccgctgc gatgtaagcg attaaactaa tgaagagata aaagtcttgt ggtgaccgct
    46501 tcactccacg gtattcaaaa gccagtttct cggtattcgt acaaaaaaag aggctgtgac
    46561 aaaattcttg tcgcagctcc ttatttcttg atgcaggaca gctttttcac aacttcttcc
    46621 ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca caatatttat aaaacatgag
    46681 aagctgtttt ttgaaattgt tccttgtggc tcagagttaa acggcaggct caagacctct
    46741 tgatacacgg tgttcaatca gctgaccctc ggtattagat tatgtcagga cgctaatttt
    46801 acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
    46861 gaaaaatata aggagctatt ttttgaaatt gctccttgtg gctcagagtt aaacggcagg
    46921 ctcaagacct cttgatacac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac
    46981 ggcaaaatat gaggaactat tttgccgttt gtttctctaa tactcaggtc aaaacgctga
    47041 tttcacaacc tctgtttttc attgtccata ggattgatat tgtccttgtg atgactcatt
    47101 cgtaataatt gaggcttgat tttcttcact tcccgttatc gtttgtccac tgccagaatc
    47161 tgaaggactt gttcctgtcg actcgttttt ttgagaagag acagttgttt tcttgacttg
    47221 ttcatttgtc tttttccatt ccgctaaacg atcggcgacc acttttttgt ctactttttc
    47281 agaataagcg gtgatcactt ttccattttt caaacgggct actttgtcgc catcatgagt
    47341 caaaagcaaa acggtcaaat gcttgtcttt aaattgatat tgtctgagag aaatcgcact
    47401 aagtacgtca tcgatattaa taaattgatt ttcttctttt ccttttggca aataaatgtc
    47461 caattcgcct ttttcgtaat tggcagttat tttatactcc gcccaccaat aagttgagaa
    47521 ttcttttttg aaattatcta ctgattcttt gcttgctacg attttttctg tttctttctg
    47581 agcaagatct gctgtcattt ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa
    47641 accgaataac caaaagagaa gaaacggtct ttttttcctc atgctttcct ccactcctat
    47701 ttaattttga ctattctatt atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa
    47761 agatcgtaaa taattgaact tctttttcac aaaatggtaa gaatggagaa tttcacgcat
    47821 ttgctctcaa ctattctttc ttaagcctca aatccgtttt tatctgatac gtctttgaac
    47881 caatgacccg attttttgat tgttcttttt ccatcttgtg ctaagtctac tgaaataaag
    47941 ccatatcgat tcttatatgc attggtccac gaccagttat ccatacatgt ccacatatga
    48001 tagcctaaac aattggcacc ttcttgaatg gcttgatgga catatttcag atgagattgg
    48061 acaaattcga tccgataatc atcgtggatc atgccatctt ctttgataaa tcgtgcttcg
    48121 ttttctacac ccataccgtt ttcagaaata aagcagtcga tatttccata atgatctcgt
    48181 acattgatca aaatatcata gattccttgt tcatagatct cccatccgcg ataaggattc
    48241 attttacgac caggcatttc ataattgtcg aaataatcct caggcattgg accgttttta
    48301 gtatcgattg gtgtttcttt tgcttttacc cggcgtggct ggtaataatt gatacctaaa
    48361 agatctactg tattttcttt gataaccgtc aaatcctctg catggatcac aggcatgaga
    48421 tcaagttctt ttactatctc aatcaattca cttggaaact cgccttttac agctggatca
    48481 aggaatgaac gattgaagaa cgcatctgct atttgcgctg ctttaacatc ttcagggttt
    48541 gattcgtctc ttggatagct tggtgtcaga ttcaagatga taccaataac accaccttgc
    48601 tccatttcat ggtaagattt gatggctaac gctgaagcca acgcttcatg atagccaact
    48661 tggatcgctt ttttcatatc tgactcttct ggataatgcc atcgatataa ataacctgct
    48721 tcgactggaa cgattggttc attatgagta aaccattttt ttactttcga accaaacaat
    48781 tcaaaacaac ttttggcata tttttcatag gcttcaaccg tttctcggtt caaccatccg
    48841 cctttttctt gcagatacat cggcatatcg aaatgataca gattcacaaa gggttcgata
    48901 ccgttttcaa tcaactcatc aaaataacga tgatagaaac gaacggcttc ttcgttgatt
    48961 gttccagatt catcaggtat caaacggctc cattggatag atgttcgaaa tgaattatgc
    49021 ccagtctgtt tcatcaactg tacatcctct tgatacttcg tataaacttg cgaggttttt
    49081 tcaggtccta cttgatcaaa gaacttttgc ggttcttttt tgtaccaata atcaaaaatg
    49141 ttctctcctt ttccatcgcc tgaaatcctt ccttctgttt gtgggccact tgctgcagaa
    49201 ccccaccaaa aatttgctgg aaattgatat tttttcatca aagcacctct ttttcttttt
    49261 tagttcgttt tagttgattc catgtatgat attttccatc acaatcactt tttcattttc
    49321 ttcctcttct tttttgattc gttttaacag ctgttctgct gctcgttccc cgattttatc
    49381 tgttggtggc gtgatcgtga ttttcggaag gatactcaga tcgtttttat ctaaataatc
    49441 atagtgactg aacgcaatat ccttcccgat tctgacacct tcttgattga aacattcgat
    49501 ggcaccaatc gacatattgt aattgctgac aaaaatagca gttgcccctt tttctaataa
    49561 tgcttttgcc ccgtaatagc cgctttttct agaatagtct cctgtaaaga cctctaattg
    49621 gatcgatgga tcattttgga ccgcttcttt ggctcctgct aaacgttctc ttgctacgta
    49681 gtctgtttga gtggcagcaa taaatccgat ttttttatgt ccttgttcac catgatgagc
    49741 gatcactttt gctacacttt ctcgatcatt cactaatatt gtatcaatac ctgctggtcc
    49801 ttctggacaa ttcaatgcga taactggaat actcattttt gtgagcattt caaatccaga
    49861 ccactcttct tcggataaga agacaatcag cccatcgatc acatgcttca tcaggtattc
    49921 aattttttgt tcaatttttt ctggctgatc ggcaaacccg cttaataata agctataccc
    49981 attttcttca gctactcttt cgatccctga gacaacttcc gacccaaaac ggctggaaat
    50041 cccgtttatc aaaagtccaa tagagaagct tcgattattc ttcaaccctt tagcaatgat
    50101 gttttcttta tagtccaacg ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt
    50161 cagttgctga ccattcaagt aacgggaaac tgttcccact gaaacaccag ccatcttagc
    50221 cacatcacgg atcgatgcca tacattctca ctcctgccag ttacatttga tttctctttc
    50281 ttgttgttcg attatgatga ccgcattctt tgcatagtgt tgcttgtcat caatatccat
    50341 ataactaaag tcttcagtag agcaacttcc tctcactatt aattatactt aaacaagtat
    50401 ttgaaccggt tcatttttaa tatagcattt ttttaaaaga ttgcaacact tttttaaaac
    50461 gctttctgaa agtggcggat tttttgcctt tttaaaattt tattctaaaa tttactctat
    50521 ttgtgggtgt tttgtgcgtt tttctcttcc atccgtttga agttttacat tccaatggta
    50581 taatttcttt atcctatcaa atgggggaag tattttgaag aaattaatcg tactatttat
    50641 tttgtctgcg ggacttttga cattgactgg ctgtgatttg tttggcggct ctgaccaatc
    50701 ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa aaagcagata ccaccaacag
    50761 tgtatatacg ctaaaagtac gtaaagatat ggatttatcc gcgggacagt tgacatttac
    50821 agataaaaaa atggaatgga cacgcacgta tcagacacaa ggaactgatt cttctgaatc
    50881 atctgaagaa aaaacagtgc tgacagatat caagatcact accaaagatg acgaatatat
    50941 tatttcagga aaagaaaata aaaaagaaac aacagtcata tttacaaaaa tcggtaatta
    51001 ccgtttgaag gatgaggatg gcaacatcta cagtctatag tgccgtctgt tggatcttct
    51061 tttgtttctc cccttctttt ctgctatagt aaagaagcaa gatgattgta aaggagacga
    51121 aatggatgat cgatagtcct gattattgta catgtaataa caacaaaaat atccatacag
    51181 aatttaatga ccaaggaagc ttgagcatat gcgacaactg tcacaaacta gtcaaagatt
    51241 catttaaaac atttgaatct tatcctgttc aaaattatgt aaaaagtaga agaagataaa
    51301 caaatccaag caaaactcac tatggctgat ttttatgaac ttcaaactca gcttcttcct
    51361 ttgatcaagc ttcatcagta aaacaaacag tggatcattt cagtttacaa atcaaagagt
    51421 aaagatttat ttatttcaaa tatttgaata taaaataacc aaacgataga aaggcttatt
    51481 ctacatcacg tagtaatttt tctttcccgt cgtttggttt tttattttta gcctaattct
    51541 tgaccttttg catgctggtt atccgtaaaa tattttccag caggctttcc ttccaattca
    51601 gctaattttt cttggatctg acggatcacc ttttttgcat cttctggatt ttctacaaaa
    51661 tcgaatttat ccccatccac ttgtatttta ggacatacat caaaatcttc ataccattga
    51721 ttatagcggg tgttcaacgt ttcataatag gtatataacg tctcatcgct ttctagctgt
    51781 tcatagtcac gcccacgttt tttgatacgt tctagcatcg tgtccaaaga aactttgata
    51841 tggaccatca aatcaggccg tttttttgga gcaacatcat ctaactcgtt tagcatcgta
    51901 tctaataagc tatcgtactg ctgtacttcg atatccgtta cccgacctaa atcagcgttt
    51961 agatggaaca acaggctatc ttcatagatg gatcggtcta gcacattgtc atcgttcact
    52021 aaagcatttt tcattgctaa aaatcgcttg ttcaaaaaga agatttgcaa taaaaaagcg
    52081 tactgctcag gatttgaata aaagagcggc aatacttcgt tatcttcgac attttcataa
    52141 aatggtttag aatcaatctc ttttgcaagc atatccgtca aactgctctt acctgcacca
    52201 atcgttcctg aaatcaaaat cactgacata caacccttcc tcttctataa gtactcgcct
    52261 acgtaagttt caacaggcaa tgtttcaaaa tcatattcac gtacagcttg gtctgatttt
    52321 aaatacgtat ggagactttg tgtaatcatc aagttcgtag gtaccgtctc ttcttgtaca
    52381 atgatcatcg gttttttcaa catagttgcc acacctaatt cgtaagccgt tcctggatcg
    52441 attgtttgat gctcaaagtc aaggatagca acaacaaatt ctgcatttgt cagttcttcc
    52501 atatcttttt cgtatacttc ctgcgcccat ccagcgctga atagttcata attgctttct
    52561 tggtgatgac gtggagagta gaaacttgtg actgttttgt tttcctccaa cgctttttcg
    52621 atacgactta ctcgatcgat ttgctcctct gaaaaaaatg gtcctgctaa atatatttga
    52681 ctcattattt tgcctccttc aataataact gatttttaat agatactgct cgtaattcat
    52741 tgaccagttc caatggattg ttggaaaaga ttacttgatc caaccatttt ttatccataa
    52801 aatcctcttt tgcaaaagtt tctaataatt gttgcaagcc gctgtaataa ccgccaatat
    52861 tcaatattcc gattggcttg tcgggcgtca gaccgattgc tatccaagaa agaagttgtg
    52921 agaattcctc caatgtacct agacctccag ggaaaacgag gacagcatct cccttttcaa
    52981 ataataatac ctttctttca tcgatcgttt ctgtaggaat aaatgtagta acatcttttc
    53041 caggcaattc agtttcaaaa agcccattcg gatagatacc tgtgactttt ccgttatttt
    53101 ccagtactgt tttactaatc actcccataa tcccggagta tgaaccaccg taaaccagtt
    53161 cgatttcttc ttgtgccatg tattttccta acgtttgcgc aataaatttg taactttctt
    53221 tattaccaaa acgggaaccg caaaaaacag taagtttcat aaaaaaacac tccctaactc
    53281 aaaagtctat cttcattcta cacgtgtttt tttaaaataa aagactagat tttgtagttt
    53341 tttagtggta tataggatag acgccactat atatagtatt tagaaaaaaa tcatttttca
    53401 gccattttat agataaaaca aaaataaata acgtgttttc atcgatatga aatgacttac
    53461 ttatagaaga tactgggatt tcgtcctata tgccaataac gtgtattatt ttaaacaaca
    53521 aatacttgtt tcttttatta acgaaaaaat cattttgttc tcgtgttatg tttcactgat
    53581 aaatgatgtt tctctaaata aaaaagccga cgaactaaac gatcaaaatt tgatcatttt
    53641 tgttcatcag cttctcctaa aaatcttttt gttaacgata aatagctgga tttgtaaaaa
    53701 tatcacttga atcgtcactg ctggaagaaa attcagtgat aaccgcacct gttgcgcctg
    53761 cctggaacta gtgtttgata ttgggctcga acgtgtactg atctctagca tgaagaataa
    53821 tttctttttt tactgtataa taactttctt tcctttttgg aatagtacag gaaagttttt
    53881 ttgtttcctt tccttcgaca taaggtatac cagacccttt cgacaacgaa aggttctcat
    53941 ttttccttca tcgtcatttc aaggagggtg ccgatgctct ggacagtttc gatcgctcct
    54001 aaaccaaaat ctgtatattc gattctttct aactctgatt ctttcatacc attcctgtct
    54061 caaggtacat ctattgtact tttttcttgt aatccattct gcctctccta actaattgtc
    54121 aaatcatccc aagatatgtc ttcttcctct ttttcttctt ggatgatttc tgcattcgga
    54181 tctactggtt ttttcaataa taaaatggtt attcctgtga caaaagaacc agctaacaat
    54241 gcaccaatga atgcccatgg tctggtcatc gtagcgactg caaacattcc cgatgccgga
    54301 atacggctat ctgcgcccca aaacatgctt aatccgccac ctactgtccc accgactccc
    54361 gttgctatga cattgcgtgc caagtcgttc aatacgatag ggaaacatcc ctctgtgatc
    54421 tgacagatcc ccataggaaa agcggtcttc aatgtatcga tttcttgacg attgagtata
    54481 tttttacgaa tgattttccc taagaaataa gcgatcgtca agccgaatgg ggttaccatc
    54541 gaagctaaaa tcaatacagt gattggttca tagattcctt ctgaaaatcg tgccacaccc
    54601 ccttatccag aataaatggt aatccagaaa cagccgtgta tctgcggtaa taagcctaac
    54661 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg atgcagggca
    54721 gctttttcac aacctctttt tttatttatg tttcataatc aatatggtaa ggatgcagct
    54781 ccgtatggta gaccgcctcc gaatattcaa tcatttcccc aatttgcgta gacgatttcc
    54841 gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat tccttgttct tcttctgtta
    54901 aaaatactgc cgagaaatca tcagaaaatg agtcaatagt atatcctttt ttcgtcagca
    54961 aacgatacaa agattctttg ccaaattctt ctctgctgaa ttgcatcatt tcttgtggaa
    55021 gataatgaac aaaataaata taaggctgat tgttcaaata atataagcgc ttaaaacaaa
    55081 cagcttgctc accgaaaaag gaaaacaaag gatcttcctt ctttatatgg accaattgca
    55141 aatccagcgt ttctttccgg acttcatatc gggagttctc caagatttgc gtaaagcttg
    55201 ccgctttgga taatcgattg taaggacgat tgcttagaac cgtcgtccca cgaccacttt
    55261 tctttgcgac atattcatct gatgccagca gttcgattgc ctttcggaca gtgattttgc
    55321 tgacttgaaa cagttcttct aattcacttt ctgtcggcaa catcgaaccg actggatatt
    55381 ttcctgagaa aatatcttct ttaattttat ctgcgacttc caagtatagt acacgtgtcc
    55441 ttttcataac gtcacccttt gctttactat tactaaaaga atagcacata aaaaataaaa
    55501 aaagaacaca aatcacatgt gagaagtgtg ttctttttca ttcagctgct ttaattacaa
    55561 gtttacgcca tttgaggcaa ttacttcttt ataccaatca aaagattttt tctttgatcg
    55621 attcagtgtc ccatttcctt catcgtctag atccacatag ataaagccgt aacgtttact
    55681 catttctcct gttgatgcac tgacaagatc gatacatccc caaggcgtat agcccatcaa
    55741 atctacaccg tctgctactg cattagccat cgcttcgata tgacgacgta aataatcgat
    55801 ccgataatca tcaataatcg tgccatcctc ttccatatga tcaatggctc caagaccgtt
    55861 ttcaacaatg aataatggtt tctggtaacg attgtataat tcattcaatg cgattctcag
    55921 cccttctgga tcgatctgcc agccccattc acttgcctca agaaacggat tttttactcc
    55981 tcccagcaag ttcccttctg aagttgctgc ttgtggatca gtctcattga ttgccgtaga
    56041 catataataa ctaaatccga tgtagtccac tggatattct tttagcaact ggaagtcttt
    56101 ttccgtttgc tctaattttt ccggatcgac tccatatttt ttatgggtac gcgctgtgta
    56161 tgcagggtat tctccacgta cttggacatc cgtgcagaaa aaattgaatt cttggttttg
    56221 gatcattgtt gctgcctgat tgactggatt ggcatcgatg ctgtaagttg ttgcatagat
    56281 aatcatgcag ccgatttgga tatccgaacg caattcgtgg ccgattttta cggctttgct
    56341 acttgcaacg aattgattat gccatgcttg gaaaatattt tggtactctc ctgctccatt
    56401 gctttttact aatccttgac ttaaagctgg aaagtgaaag gccgaattga tttcattgaa
    56461 agtcatccaa tatttgactt tactgctgta tctctctaat actgtctgag caaatcgttc
    56521 atagaagtcg atcagttttc gatttttcca gccaccgtat tcttttgcca aatgaagagg
    56581 catttcgtaa tgagaaatcg tgatcacagg ttcaatatca tattttaagc actcatcaat
    56641 cagctgatca taaaattcta atcccgcttc gttaggagta ccttcgtctc catttggaaa
    56701 aattcgtgtc caggcaatcg aaaaacgata acatttgaat cccatttccg caaacaaggc
    56761 aagatcttct ttgaaacgat cgtaatgatc gattcctcga tgattcgggt aaatatattt
    56821 ttcttgatca atcgtccagt caaattcttc actgccgata atcgcaaagc gctgcttacc
    56881 tccaggcata gcatctgcaa cggatagtcc ttttccgtct cgatcataag ccccttctaa
    56941 ttgatttggc agcagttgct ccaccccata aaaatttttc tgaaaaacgt gattgcatcc
    57001 tgatttcctc cttattttac aattgccaga acatcttcgt ttgcaataat ctctttttgg
    57061 tctaattcaa gtatatcggc aaatgcatca gtattcgtaa tgacaactgg agtgattacg
    57121 ctatagccag ctgctctaat tgcttcgatg tcaaaacgaa tcaataaatc tcctgctttg
    57181 acgagatccc cttgtttgac taagatctca aagccattcc catttaattc tactgtatcc
    57241 atcccgatat gaatcaaaat ctccgctccg tcagtcgttg tgatccctac agcatgtcca
    57301 gttggaaaaa tagtcgtgat ctctccgtct gcaggtgcaa ctagttctcc tacttctgga
    57361 tcaatagcga taccttttcc catcacgcca gaagaaaaaa cttggtctgg tacttcactc
    57421 aatggcagta ctttacccgt taaaggactt tttaaggtat gccgtgctga agtaatcggt
    57481 tctcctgcat tcgcatgttt gttttctaat tgattgtctt gtgtttgttc gtcaaatcct
    57541 aaaatcaatg tacctacgaa tgcaagaaca aaagcaattc ctgtagctat tactgctact
    57601 gtcacatttg actctacacc atccaccgta ttaatgaaag tcggaatcgt caataagctg
    57661 accaaactgc tggagaaagc ttttactccg gaaaatccga taatagctcc accaatacct
    57721 ccagaaatac aagcggcaat gaatggtttc ttcaaaggta atgtcacacc atacacagtt
    57781 ggttcagtga ttccaaataa cgaagtcact gtagaagaaa tccctaatgc tcgaagtttc
    57841 gtatcttttg ttcgcaatgc tacagctaaa gcagccccac cttgtgccaa gatggctgga
    57901 agaagcattg gcatcaatac atcaaaccca tattgttcga tattcaaaaa catgatcggt
    57961 acgaatcccc aatgcatgcc gaacatcacg aatacttgcc ataaacttcc catgattaga
    58021 cctgctacga ttggactaaa tccataaatc gagttgaaga gaccgcctaa agcattaccg
    58081 gcaaccgtcc cgattggtcc taaagcaaga aatgttaatg gaaccatcac tactaaaacg
    58141 acaagcggaa ctaaaatcat ttgcagaaat tgcggaatca ctttttttac gaatggctct
    58201 aatttacttt gaacccatac ggctagaatg attggtatga cagaagaagt ataaccagat
    58261 ggtgaaagaa tcactgggat cccaaagaaa ctgatagctt ctcccgcacc agcaactgct
    58321 gtgatagctg gatacacaag tgccatagca attgctactg ctaaaaactg gttggctttg
    58381 aactttttag ctgctgtaaa agcaagcatg acaggtaaga aggtaaagac accatctgct
    58441 gcagcaaaca atatttgata agtaccgcta tcagctgtca gccagcccat caccgttgcc
    58501 aatgataaga atcctttcaa taccccggcg gctgccatcg cccctagaaa aggtgtaaat
    58561 atactagaga taatatcgat cagacgattc aaaatatttt tggactcttc ttttccatcc
    58621 tccgaaactg caagaccttc ctcatccacg atcgattgat aaacatctgc tacattactg
    58681 ccaatcacta cttgatactg accgccactt tgtacgactt ggatcacgtc cgggtcttct
    58741 tttaactttt gcgtatctgc taaagattca tcttttaatc tgaatcgcaa ccgcgtagca
    58801 caatgtacta aacttttgac attcttttgt cctcctaccg cttcccatac acgacgacca
    58861 actgcttgat tatccattgt tttttccttc tttctttttt atttaaaaaa taaaaaccca
    58921 gattgaaaaa aacagtcaca aacattcgtg aacctatttt ttcaatccag gttttgcctg
    58981 ccatacagta acaatcctta tgattggatg ctttttttct cacttactac tctttgaata
    59041 tgaatcgtta aataaatctg ctctgcgatc cccatttcct gtttttgcgt ttgtcgaaga
    59101 tactcattga tccgttcgac acaatgaaac gcttttggat actttccttg aactaatgcg
    59161 tagagaaatt catcttgctc gtcagatcct tcccctttga gataacgctg ggcaaaaaac
    59221 tgcagatgag taatgatacg ctgataattc aaagattctt catcgaacac gatgccgaag
    59281 aatttactga tgatactcag tatatctctt accattgtgg tagcggtcat cgtcacttgc
    59341 atgtcttcgt ttccttgttg gctattcaca aaatgaaagg caataaaacc ggcttcatcc
    59401 tcagaaagtt ccacacccgt cttttgtttc acgaacaaaa gagcctcttt cgctgcttga
    59461 tattctttcg gataaaattt ttttgtttca aataataatg gattaggcag ttggatattt
    59521 tcttttgctc gtgtgatcgc ataatgaaga tgatcggtca atgtcagata aatattaggc
    59581 gaaaattcaa cttttagttt atcttcagcc agttcgacta cttgtttgac ccattcgatc
    59641 tcctctgccg gaatatcagc catcagctgc tggaattggt ttttagcaaa agaatctctt
    59701 aaaacaaatt ctttttggac cgcttctggc gaaatctcgt ccccaatctt tttttgaaaa
    59761 gctagtcctt ttcccataca aacgacttct tccccgaatc catttttcga aatgacgaca
    59821 ttgttattca aaattttatc aattttcatt ttgcttcgcc ctctcaagaa aaataaaaaa
    59881 acctagaatt cgcctactgt ttcttagctg aattctggtt ttgcctgccg tacagtaaca
    59941 atccatttct tattcaaaat catcatacca tcgaattttt atttatgcaa gccttttcat
    60001 ataaaaaaac tggaacacaa gtctatctag aaaacaacca aatcagagat gcacctcttt
    60061 gcgtttctct ttttttcagc aaattggttt ttccttatcg attcatatcc tttggctgtt
    60121 tttaatctgt aacgacttat gtcccagtat cctaaaatgt ttttgtctct agattgaaag
    60181 cagatctcat atcatctgtc gcatctttta tcgatgtaac actttccagt tctccttgta
    60241 caactagtcg cgtgacggcg tccatgtcgc cacaagtaat tagtgtgaca atttcttttc
    60301 cctcgacttc attcagtaat tgggtatctg taggagcaac tttttcttta aaaaatgttt
    60361 tgtatgtata cacattttct aaatctgtca aatagatctt ggcacccatc tctaaatttt
    60421 ctagcggtgt aaacagcaat tcagggttcg ttgcacgatg gcttgctaag gcataatttc
    60481 cttttcccat ttcttgatct ggagataatg ttcctgctcc gtaaagtagt gacgtattat
    60541 ccaagccttt gaaaaccggt aaattgattg aaactgaagg gactgcgata ctagcaatca
    60601 ctggtagatc tgttcctctc atttgcgacc tcatgacccc ttctgtcgtc attggttcta
    60661 ccgcatcaaa gtcaaaactg acatctttat cgttattctt tttcagatct tcttttgtta
    60721 cattcgctat agcatattta tctcctgtct ctctgacaaa ataatccttg atctgttcat
    60781 tgaaaatcaa agctagtcct actaataaca agaggaacaa gaggatattg actagccatc
    60841 tttttgcttt tccttttttt ttcattttct ctccctccag actcatttta ctgtttttct
    60901 tttgctcttt ccaacagccg aatgacacat ttctacaact ctgtgtattt ttttcgtaca
    60961 cctgcgcttt tttctacagg atatttcaaa ttatttttga tcaatttttc ttcaatgatc
    61021 tcatctagat ccaggtgaag gttgtctgcc atcatgtatg aatagatcaa tacatctgcc
    61081 agttcttctt tgattcgttc taattgtgtt tcttttactt cttccggcgt tttccattga
    61141 aaaagttcta acaattcaga tgcttccaaa gaaatagaga tagccaaatc tttttcgtta
    61201 tgaaattgtc gccaattacg ttcatctcta aactggttga ctttttccat actattcatt
    61261 tgcttgcctt cttccaatca cttcttactt gattaatcat accatttttt tacataaaaa
    61321 atagatgatg cttttaaaaa aactcctgtt ttcttttatt taaaataaat gtcatagaaa
    61381 atcaataatt taggtagcca ccatgtgtat gcagtagcgt gtcttcttcc cttgaggctt
    61441 tttgtttttc gatacgttct tcttcccaaa cagacaacgc tactagaatc tctcttaatg
    61501 attttccttc ttcggtgagc gagtactcta ctttaggcgg gacttggtta taggcttttc
    61561 gatggatact ttcatcttgt tctaactctc ttaactgctg ggtcagcatt ttttttgtga
    61621 tcgtagggat ttgcttgctt ctccacgcct aaaataagcg atattgctct tccctttatc
    61681 tggtgtaagc aaagcagtta gcattttgac gatcgtgggt tccgcttttc acttcttatc
    61741 tacttagtat catgaagtat actttcgtac taatactaag tagcggaagt gtcaactact
    61801 ttgtttaact acatagcaaa agaaacagaa gggtgacaac ggtcatcacg cttctgtttc
    61861 ttttgaatat gctgtgttgt tgtctctatc tccgtgtaga ctttcctcaa acttatcctt
    61921 tgacagtaag tcagtaggct gatttgctct ttctagatgc cagatacatt tctacaaaaa
    61981 aatagtgatt ctgttttgaa aattcgattt ttagttctgc attttcgtaa acatccaaca
    62041 tttttttcgc actgtataga cctaacccgc catttttttt agaggttttt cctcttttga
    62101 ccatctccga tagcgtcaaa ttcgttggat ttgccttgtt cgctatacta aaacgtgatc
    62161 cctctttttc ttccagcaga ttgatcgtaa tcggactctg atcggctaca cgatgatcga
    62221 atgcgttaga aataaaaata gataaacagc gaataaaaac aaccagatca atcgggattg
    62281 ttttcacttc gacaggaata actacttgga tcgggatttt ctccgattct gctttttcta
    62341 gaaattttaa aaagacaccc ctgacagctg gggatacaat atttgttagc tgttgtattt
    62401 gcggtttttc aaaaatagct gcagaatgat cttctaatat ttctaaaaat tccaacgttt
    62461 cttttttatt ttcctgaaga gtaattttta atgaaaacag tagactttta taatcatgtt
    62521 tgaattcctc tagatgatgg taatattctt catttatttg atacagttga agcgattcct
    62581 gatgttgtct ttcttttact tggaaactga tcaacataat ggaaaaaaac aaagaaaaaa
    62641 aactgatgac tatcgacaaa agagatagaa gcagataatt agatgagcgg aagtgaacac
    62701 ttcccaatac caagtaaatc acgccccagc ttaataaaat acaaatacct ccaaaatagt
    62761 gttcagttgg ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct atacttttta
    62821 ctaatagata gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt ccaagatgtc
    62881 tctttcctaa ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca gtgatagcaa
    62941 aaaaaagatt actgatcaaa cagctgataa acaggttttt cgtagtaaaa taaaaaagaa
    63001 acatcaataa taagccaact aggtaaggaa agctcacttt agggatagga agtaaaagtg
    63061 tcaccaccat caaagctata caaccaaagc tgttttttac tagcagttgt ttcgaaaaga
    63121 ataaatagat acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc actgttgcga
    63181 ccatcttatt cctccattgt tttgtttcaa aatgtacaca tattttttta ataggagcct
    63241 gcatatgaaa aagtatttta tcatagaaga taaccagttt cacagcgaat ggatcatcag
    63301 agaattggtc aatcatcata tcgagaatat cgtgcagctc acttctatct ctcaaatcaa
    63361 tcagttgatc cagttgggca ctattgataa tcacgatgtt ttctttatcg atatcgactt
    63421 gaagcaatca ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa atgcacatag
    63481 taatattgtt tttttcaccg cttatacgaa ttttgcagta gagatcatca atcggaaaat
    63541 ccttccgatt ggatatatca gaaaagacag gaatttatct gaacaaattc gtcaagtgct
    63601 agaacagatc aattggaaag aacagcagat cgtaaatgtc gataaaatct ttattgatca
    63661 aaaaggtgga agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa gtatcaaagg
    63721 tttgaaaaac caagttatgg tcaaaacaac tacagaagat aaattagtga atacttcttt
    63781 aaaacaattg aagacagagt tagaaaagta tgcttacttt ttacttttaa aatcttactg
    63841 tatcaactta tcgcaattgc agaaaattga ttctgctcat tatgtgttag aatttttcaa
    63901 cggtgactcc ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac gtttccatga
    63961 cttccaaaaa actgcttcga gttgatgaat aagagctgag acgtaagcat agcagatttc
    64021 agggagtggg ccttgaatcg gctccactcc ctgaaatcgt tgatatgatt ttacgcttcc
    64081 tcttctgtag gagcatctgt taacgaccat gtgaatgttg aaacgtagtt gtctacagca
    64141 taattggata caggaacaaa taattccact gattgtctcc cttgttccac attctctcct
    64201 aatgaataaa tccatgtgcc aatcccttca tttgctcctg cagttactag atctactgcc
    64261 tgatctgctg ggatttcgat tccttcagcg ccagttccag aaacggtaga tggcattcca
    64321 ctgctattag ttgcgctatt tcgaatcacg gcgtttttga atttcacctt tgaaccagtt
    64381 agtttattcg tatcagatgt tgtaccaata aattctgttc gtttgactga taatttccaa
    64441 ccggcataat ttccgctttt atccgttact tgtacgaaat tctgtacttg tgtttgtgct
    64501 ccagtactgt ccgttccgct ttgtaatgca gcatatactg tttttgctgt acctaacttg
    64561 attgttccaa aatcaagatc tggagcttgg tcaatcgtca gtaatccgga tgttccctga
    64621 tttggttcgt caggtgtaaa aggagtatca ggatcatttg gatcaacagg aggaactgga
    64681 acaacgtctt caggatttgc agcatctggt ttggtcacag aaatcgttcc ttctgtagtg
    64741 gctgagctct tattctcagc aaaaacactt tgtgtacata ataatagact tccagctaga
    64801 acggtactta atgcaattgt ttttttcatt tctttttcct tctttctttt aaaataaata
    64861 atccagcaaa taagccaata agaatgactc caacaagtat cagccatcgc cagtcaaatg
    64921 tttctggctg attttttaaa tcaatatcct tttcattcaa ttggtccgct gcatcatcag
    64981 ttatttcaaa atcggtatgg agattccagc gtttttcttt ccaagcaccc ttcacaaccg
    65041 cttgataatg tcccggagct aatttttttc cttctaatgg gacaagtaaa tcaaaatttg
    65101 tattgggtgc tacatctatt ttttcttttt ttattgaaag gtaaggctct tttgtttctt
    65161 ttttgtagat ttcaaccgat gtagtcaagc cgaacatata agaagcattt ggattttgaa
    65221 gattcgcctt aatcacattt cttccatcgc tttgatccgc ataagctttt tttaatttca
    65281 aatctgggga agactctttt tcgctatttc aaatcaagat tgctttcact tgagaaaact
    65341 gattgacgac accaactgta cttttgttag aaacgttctc ttttttttct tgaaacgtga
    65401 tccctcctgc gaccacacca gccagctctt tttctggcgc tttcagtgaa agtgtctcag
    65461 tgaccgcact ttgaggagga acagttattt ctcccactgt ttttgctaag tttttcatat
    65521 cataacttaa tgtttcatca aatttacttt ttgattctcc atattcgatc acaccgtttg
    65581 aattcgtcgt agcggagtgg atcaatgtct caactgttac tgatttatct gttcggttaa
    65641 agaccgtgta ggggatttcc tgtgttttcc caggctccat ccttacattt atatatgttt
    65701 tgttcttatc aatctgatta gccggtaatt caaaatctac cgcaaaattg aattcggagg
    65761 catggacagg tgtctgccaa aggatgaatc caaggagaag gatcataagt aaagagcagt
    65821 tgtttcgatg acttttattc catattcgat tcatcaatca tcctccttga tctttattct
    65881 gtcggcgcat ctgctaatgt ccatgttatc tctgctgagt aattaccagc tatctgattt
    65941 tctttggtta agacaagttt aaacgcccga ccgttattta aatattctga ataatctgct
    66001 tgcacattcg ttgaattttg tcctctgaca atttccagtt cttcttcatt cactaattga
    66061 tactcctctc ctttcttgaa atataggaca tcctgcatag aaaatcctgc atcatttgtg
    66121 aaaggctgag ataattttat cgttaagatc cacggatttt tcgtttctcg ctgatcataa
    66181 atccataatt tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa accagttgct
    66241 ttgatctgac caaaatcgag tttggaaggg gcaagaaatg agagaccacc ttctactacg
    66301 atcttcgtag caggtgcaaa aggcggggca tcatgataaa caactggcgt atcacttgga
    66361 ttgtgattcc caatttcatc gttggtactt ggcttgttct gcacaccctt agcagtagct
    66421 tctcctgctt ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag ttgattttct
    66481 gggatagaaa gaacaaattc ccctgtatca tcaatagttg tttctgacaa aacagcttga
    66541 ttttttttca atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt gcgagaagct
    66601 gcattgatca atgtggtact taaattagca ggttcaggag gaatcacatc gactgtcgtt
    66661 ttggcagagt ccagttctgt cacttcttgg ccacctctta cttttttagc agcaacaact
    66721 tgcacttgat cctccgtttt taatctctca tcattgggta cttgaacttc gaacaagccg
    66781 ccttgttcgc cttctcccca tgggttatat cctgcccctt cattttcatc tgtttgtcct
    66841 tgcgtgagag cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt aatacgtaaa
    66901 gtcacttcta cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca
    66961 acgacatgcc catagatttt tttatctgca ttcgttggca ctcttaagga ttcaatcatc
    67021 gggagctgat tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca
    67081 aattcatttg ttaaacctgc actagggctt gatacgaaat tgcctaagtc ggtcccattg
    67141 atcgataagt ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt atcgaatgtt
    67201 gcgcctgttt ttcgccataa agcgatttct gaatgggcga tctttaaaga agaatctcca
    67261 tttccttgga atacccagcc gcctatgcca ttggtctcgc ttccaatccg tttgttttgg
    67321 aaatcaaaat aggtaggaga ttctaagtcc acattcaaca catccccact gatgatgccg
    67381 gtcttatctt cccctgtttt cccgaccatg actaaataag ttttttcacc tgccgtaaaa
    67441 tttaaatcaa tatctccagt tgtatctaca gttgctccgg aatctgcgcg tatatcgata
    67501 tttgattttt ctccagtgac tgtaaaatca gctgaacctg gactttctag aaaaccagaa
    67561 gcgtcataaa attggatcgc ttgatttcct tgattgagtc tgttcacgta agatttgcca
    67621 tcacctgtat ttcttaccct caatgagcct ccattggtta ctgtcagctt ttgcgtacca
    67681 ttctcaaaac gaactgcgct ggaacgccca ctctgcttat cgatgtcgat aactgatccg
    67741 gctccttcta cgatcaaaga aaggcctttt cctacaaaac gcatcccatt gttcccgggt
    67801 tgatcgtcat ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga
    67861 ctagaatctc cttgcaagcg aaatccgccc gtagttgctg tattgatggt gattttccca
    67921 ccattcgata cagtgacttt cgatcctgaa cctctcattt ggatgattcc gcgattgttg
    67981 tcattggtaa tattgatctt tgtatttacc gaaaattcgc ttcccgaacc actgacttca
    68041 aacatgacat taccaggtag cgtatcgact gcctctcctt catcgctaac gccatctgcc
    68101 cgatcataat caagagagac ctttgaaccg cttaagatat ggattcctgg cgctgttttt
    68161 ccactaccag tctcatctcc tgaatctaga aaaccgtaga atttccctag atacggtcca
    68221 taaagttgac taccattgat cacattcaat tgatagcttc tacaaaaata ggctttaccc
    68281 ggagcggcat tcagtccttc cttcactggt tggacatcca acacaccgga aacaccatct
    68341 aatgtgacag ttgcacgagg agcgtaaatc agtcctaatt tggacccatc atacaaatca
    68401 agtgtcccgg taaattttat ttctcccgta aaatgatatc tgcttcccca aaaatcggtc
    68461 atataagcaa tgataatggc atttccatcg atatttggtg tcaatccacc tggatgtgca
    68521 gctgttcctt taaaattctg gaactcgatc gactgaggta tctcattgat ataaatatgg
    68581 tgtgtgccga aattgtgagc gaatccacca aaatcaataa cgatatctcg tttgatctct
    68641 aattccctgt ctaaagtcaa aggctgaatc agtcggatct tagttactga ttcgtctgcg
    68701 agaagctgcg ccagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacagct
    68761 cttgccagtg ccgcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt
    68821 gtcttatttt cttcttgcct tttattagta gaagcctcac tttgagattc tgtagtttca
    68881 gaagtaatcg attcttctga aaaagtagtc ttgtcaaaac tttgattctc tgattgcgtt
    68941 gattcgtttg ttttctctac atcggcaaaa acggatgtca ccatcaagag agggaagaca
    69001 aacaaaatcg acgcaatcag cttttttaca cattgattca ttctccacca tcctctctga
    69061 cgttttgaca attttatttt atcgttttaa tctatttcca attgtacttt catatttaaa
    69121 atatgtattt ttatggaaaa atttttctat taggtgtaaa tttctttttt gtacaaaaaa
    69181 aaggctgtga caaaagtctt gtcacagtcc cttatcccga acaagcggtg ttctcccgcc
    69241 aactcctcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gcaatacttt
    69301 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
    69361 atcagctaat gacacaacta attgctcaca agtaaggtag gctagatttt tcgtagattc
    69421 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
    69481 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
    69541 accgccgact gagttagtta gtcctatcag caaggtactg ctctcaattt cttccttctt
    69601 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
    69661 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattga
    69721 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
    69781 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
    69841 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
    69901 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
    69961 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
    70021 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
    70081 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
    70141 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
    70201 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
    70261 aaaacatcca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
    70321 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
    70381 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
    70441 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacggcctt
    70501 gaataataaa tgcgacaatc cccgctataa ttagtgtgcc taacaccaaa acggaaaatt
    70561 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
    70621 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
    70681 tttatctact tctacgtgca ttttttgata ttgttttctt tatttaagat tatacagaaa
    70741 aatatctagg gtcgctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
    70801 aagtagtaat taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt
    70861 attgatttaa atagttcata ttttttattt ataccgctta tttactactt gctgtactag
    70921 aatctgaagt gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag
    70981 cgtctttcaa tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt
    71041 ctgttgcaat ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt
    71101 ttttcaccat tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga
    71161 tcacatcaga aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa
    71221 ccgttgtagt agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag
    71281 ttgacttgtc tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat
    71341 cttttgcatc atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag
    71401 attcccaagc cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt
    71461 gaaaggctag actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt
    71521 tcaattgtga atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgagaatcga
    71581 tttctttggt tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact
    71641 gttgcacagc ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc
    71701 tgattgtacc gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca
    71761 ataagccagt gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct
    71821 cctacttttc gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa
    71881 gaacaccttt actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga
    71941 aagttcgctc agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt
    72001 tgttaccgtt tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca
    72061 aatgttatcg attttgtttt tcttcttccg ctgctgggat attcaaatca gacactttta
    72121 tgttcgattt tccagacatt tttttgttgt ttttattatg atatattttt atacaactaa
    72181 aaatgaatat attaaaacta ccaaaaataa cttttaacgc tgtttcatta tatgtttttt
    72241 ctattttata taaacttttg gatacttaat accaaccggt tggattgtac caacatgaga
    72301 atgtgtcagt tctatttcct gtcgcacttg cataggccca tactcctttt gttgcatttg
    72361 ctgatcgaat agagtttcca actttcgctt ttgcaccgtg acgattatag acacgactat
    72421 agtggttaga atgacctgtt tttctcgtac aagttgtatc actataataa acccatcttc
    72481 cagagctatg attaattgct gttctagaaa tttcccggtc tactggtaaa tttgacttaa
    72541 taatattacc tttagcatca aaatgaatct ctcctggctt taaagatttg taatagattt
    72601 caccgtgaac tgaatttgct tccacttcag atgacatagc gtttcctcca acaaaaccta
    72661 tccctagtgc acaaataata actgcttttt tcataaaacg acatcctttc ctttttgatt
    72721 tttaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc gtgactacga
    72781 taactaacgt accttgggaa gcttgttgcg ataagatttc aactacttta tccttatttc
    72841 ttttatccaa tgacccagtc ggttcatcgg ctaaaatgat tttgctattc ttcaataaga
    72901 ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata ggcgtactta
    72961 gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc ttatctgaaa
    73021 catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt tcatattcaa
    73081 tcaacccaaa atcttgaaaa agaaaagata tggtatttct ttttatcaag cgcttctctc
    73141 tcccagttaa ttgtttcgtc tcttgatcga acaaatagaa ttgtcctgaa ctgaactgat
    73201 cattcaatcc taatatattc aataacgtac ttttacctgc tccactttct ccaacaacag
    73261 tcacaagatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt ttgtttcgaa
    73321 tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct ccattcatca
    73381 gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga ataaaatcaa
    73441 tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata agaaaaagga
    73501 agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct ttatacgttt
    73561 caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg tagacaaaat
    73621 atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt aaaaatatct
    73681 gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca gtctgagtat
    73741 acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc tcaaataacc
    73801 tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct ccgctgagaa
    73861 tatttctttc caaatacata ctgtttttca atgtcagtat ttcaacaacc ggataatgtt
    73921 cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt ataaatagaa
    73981 atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc aggtcatttt
    74041 ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt tctttttcgg
    74101 ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga taattcagat
    74161 ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct gatacattga
    74221 ctaatatact tccgttttta ttcaaagttt catataattc ctgatctgct gctgatgcaa
    74281 aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga taaaatacat
    74341 gatagtcctc accagcgatt tcttttggtg gttcatggga aacagctaac tgtgaagaag
    74401 aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa aacaacaatc
    74461 caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa gcgatttttt
    74521 caactaataa aagagaaatc aaagagatac catatattcc gctgaacaag aaaacgtaca
    74581 tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca ccaatgattc
    74641 ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac aatacttgat
    74701 gtaatgaaaa gtcattattc aacaataaac tgatttcttt attcatcaat aagaaaaagg
    74761 atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga taattatata
    74821 cgtctgtatt tgaaataacc gaatacgaat agctattttc aaagtcttca aaaatattag
    74881 tggcataatc agtgtgaaat gcttgattga atcgatcagt gaatagtgaa atgaaccgtt
    74941 cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct tttattttga
    75001 tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc ttctcgtgag
    75061 aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt atcttgaagt
    75121 tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa gaaagctcag
    75181 tttctgatga gacattttca aataccatga ttagctgctc atattcttct tgcccaataa
    75241 aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat tcatttgtcc
    75301 gatagagtac ctgtttattt atactaacca aggcaaaacc actcatgata ccggtcacta
    75361 tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct tacttaaatg
    75421 attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa gtgtataggt
    75481 catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa caccggagaa
    75541 aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg cgaaaaaatt
    75601 ggtgtttccg ataaaacgat ttctagttgg gaaaattctc gtacaatgcc agaccttgaa
    75661 atgctgtcac ttttacatca tactttaggt ctgccgattg attttccttc tattgaatca
    75721 gctaccgaat caatagaacc aacattgcca gatgaacctc ttgttaacaa gaaatcttat
    75781 tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct cgtcttttgt
    75841 ttggtctatc tttttcttat caattatcaa tacaaaaatg aaacgatcga tcgattcaat
    75901 ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt ggacaattcc
    75961 atcgaacgtt gggtatgtaa tgatatcttt ggccatggta catggctgtc ttttcacgct
    76021 ggagaggtag aaaaagagaa aaaccatgct gtaatcaaac acaaaggaaa acacgtcatg
    76081 aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat cctcagccaa
    76141 gactatgacg acgccttaaa aagaagccct caggagccag ataggcatta agtaggagat
    76201 gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc caaaaaacag gggtacaaat
    76261 taattgaaaa agccttgaga tgaaagttcc taaataaaaa agttcagaat gataggtatt
    76321 tctggcttat agtataatat ttacgccaca gcctcccaat gattcttttt ggaatcataa
    76381 tcaaggatgg acattcacaa aaatattttg ctcattcgat ccgaatagat taacgaccaa
    76441 tctagtaagt aatatagatt ggtacgtagt caagctgcac ataatttaga tcaattttag
    76501 ttctttttta tatttctatg atctgctcat ttacttttat atgatggctt acagtaacat
    76561 aaataaatat gataatcgct ggtagtttat ccaagagtac tcaataaaat acaaaatgga
    76621 ttgttcaagc gcctatcata atgataaaat gaaacaaata aacgatacaa ggaggaagag
    76681 actttgagta atggcagaac aattttttca ttgtaaaaat tgttcagttg ttaatctatg
    76741 ggatttcggt agcatgggca tttttttata gaggaatcac accaacctgg ccgttttatc
    76801 tcttgttagt gggaatatta gtaggaaatt ttgcccctct tcactatcgt acgaactttt
    76861 ttccaactga ttctggcctt tttcttaaaa caaagacgcg aaaattagaa aacattattg
    76921 aaatgacaat tacctgtata atcattttct tagttatatt cctaaacgtc ttctaataag
    76981 aatctcttaa aattagcttg ttacagttag ttacctagtt tatataacac agttatctta
    77041 tcttccttat caacatcttt tgccaactct ttgtcttaaa ttcgaaacat ataagcagag
    77101 ctacgaacac caagactgtt tcggctctgt tttttgcatc aaattcactc ttatatagct
    77161 gataatctcg gctctttaaa agaaattatt ttcgcttcat tttattataa aaaggctagg
    77221 aaccttgatg ttatcaggtt cctagccttt atttaagtat taccccacag acccttccat
    77281 atagattttt aaattacaca ctagtttaaa ctaaaacaaa caaggaaaca cttaccattt
    77341 aatcataaaa ttagctcata tgaatatatt agaatgtaaa atagactggt caaaatctgg
    77401 tcaaaaaaga ggactaatac aggtagtcct cagactgtag acaaagtcca ttttatggat
    77461 gat
//
LOCUS       DAPBBG010000006        74757 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPBBG010000006 DAPBBG010000000
VERSION     DAPBBG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 74757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.006"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..1644
                     /locus_tag="RD477_000459"
     CDS             49..1644
                     /locus_tag="RD477_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7632851.1"
                     /translation="MIDTVRDFILYLKLCNRTNEIIESKVNAIVIKELKENSLKRSFA
                     EEGLKIHNEGDKCAFCGGEVTAERIVELESVFNTKEVFRLQDRIKQGIAKIEECIDIL
                     SNIDILNASDFYSHLDTEKVNSRLIDIKKEQMNFLKECKMQLEKKEKELFISMDSFEM
                     KIPLSFTTIQKDLNELIKSHNEFSNDIEKSKSEAKRKLILHRVAEKCEQMGTERLQGE
                     LATLKKTSTDLKKILSDEIEKIESDKRKIESEIIEQRKELKQLQEKIKNPEIIIKNIN
                     EKISKSGKKNLELKYIESGKHYKIINKDGSTRNIQEISTGEKNIIAFLYFIGSLDSPE
                     LETGKPKIIVLDDPMNSNDDTMQYLIISEIEKLYSRKNLFKHFILLTHNSHFYLKVTF
                     GRRVRRDGKNPYEMDNFVRMINDGNLTSFKYLKHKNEDFTTQYGSLWKELKFLYDNDK
                     KDFMCNTIRRIIETYVVFNGVSGNKDAESKMLFNTNSHYTEIGDLETDINGYSKEDII
                     GLMRKFFKQNNAEVHFNNYWNKL"
     gene            complement(2299..3444)
                     /locus_tag="RD477_000460"
     CDS             complement(2299..3444)
                     /locus_tag="RD477_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7632852.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(3505..4152)
                     /locus_tag="RD477_000461"
     CDS             complement(3505..4152)
                     /locus_tag="RD477_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7632853.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            4346..4639
                     /locus_tag="RD477_000462"
     CDS             4346..4639
                     /locus_tag="RD477_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632854.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            4683..4994
                     /locus_tag="RD477_000463"
     CDS             4683..4994
                     /locus_tag="RD477_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632855.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            4991..5615
                     /locus_tag="RD477_000464"
                     /pseudo
     CDS             4991..5615
                     /locus_tag="RD477_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            5756..5914
                     /locus_tag="RD477_000465"
     CDS             5756..5914
                     /locus_tag="RD477_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632856.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            5915..6277
                     /locus_tag="RD477_000466"
     CDS             5915..6277
                     /locus_tag="RD477_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632857.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            6314..7162
                     /locus_tag="RD477_000467"
     CDS             6314..7162
                     /locus_tag="RD477_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDT7632858.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            7152..8627
                     /locus_tag="RD477_000468"
     CDS             7152..8627
                     /locus_tag="RD477_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDT7632859.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            8893..9300
                     /locus_tag="RD477_000469"
     CDS             8893..9300
                     /locus_tag="RD477_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDT7632860.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            9303..9518
                     /locus_tag="RD477_000470"
     CDS             9303..9518
                     /locus_tag="RD477_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632861.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            9522..9902
                     /locus_tag="RD477_000471"
     CDS             9522..9902
                     /locus_tag="RD477_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDT7632862.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            10036..10191
                     /locus_tag="RD477_000472"
     CDS             10036..10191
                     /locus_tag="RD477_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDT7632863.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            10260..10733
                     /locus_tag="RD477_000473"
     CDS             10260..10733
                     /locus_tag="RD477_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDT7632864.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            10730..12424
                     /locus_tag="RD477_000474"
     CDS             10730..12424
                     /locus_tag="RD477_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDT7632865.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            12390..12575
                     /locus_tag="RD477_000475"
     CDS             12390..12575
                     /locus_tag="RD477_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632866.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            12579..13754
                     /locus_tag="RD477_000476"
     CDS             12579..13754
                     /locus_tag="RD477_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7632867.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            13747..15270
                     /locus_tag="RD477_000477"
     CDS             13747..15270
                     /locus_tag="RD477_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDT7632868.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            15326..15610
                     /locus_tag="RD477_000478"
     CDS             15326..15610
                     /locus_tag="RD477_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDT7632869.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            15597..15932
                     /locus_tag="RD477_000479"
     CDS             15597..15932
                     /locus_tag="RD477_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDT7632870.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            16227..16853
                     /locus_tag="RD477_000480"
     CDS             16227..16853
                     /locus_tag="RD477_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632871.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            17600..18436
                     /locus_tag="RD477_000481"
     CDS             17600..18436
                     /locus_tag="RD477_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDT7632872.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSIAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSGGAVVGGIAGNIVFEVFLRLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWIWYLICGGILGFLASFLGIGGGPI
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSGFMRFDLSMLFYIIPAAVI
                     GGLWGAKFSRILSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            18501..18935
                     /locus_tag="RD477_000482"
     CDS             18501..18935
                     /locus_tag="RD477_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7632873.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(19003..20325)
                     /locus_tag="RD477_000483"
     CDS             complement(19003..20325)
                     /locus_tag="RD477_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDT7632874.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(20479..22119)
                     /locus_tag="RD477_000484"
     CDS             complement(20479..22119)
                     /locus_tag="RD477_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDT7632875.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            22449..22919
                     /locus_tag="RD477_000485"
     CDS             22449..22919
                     /locus_tag="RD477_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDT7632876.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            23548..24651
                     /locus_tag="RD477_000486"
     CDS             23548..24651
                     /locus_tag="RD477_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDT7632877.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            24935..25615
                     /locus_tag="RD477_000487"
     CDS             24935..25615
                     /locus_tag="RD477_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7632878.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            25705..26739
                     /locus_tag="RD477_000488"
     CDS             25705..26739
                     /locus_tag="RD477_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDT7632879.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            26753..27088
                     /locus_tag="RD477_000489"
     CDS             26753..27088
                     /locus_tag="RD477_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632880.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            27072..29552
                     /locus_tag="RD477_000490"
     CDS             27072..29552
                     /locus_tag="RD477_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632881.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            29608..31167
                     /locus_tag="RD477_000491"
     CDS             29608..31167
                     /locus_tag="RD477_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7632882.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            31164..32528
                     /locus_tag="RD477_000492"
     CDS             31164..32528
                     /locus_tag="RD477_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7632883.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            32699..33769
                     /locus_tag="RD477_000493"
     CDS             32699..33769
                     /locus_tag="RD477_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632884.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            33784..34452
                     /locus_tag="RD477_000494"
     CDS             33784..34452
                     /locus_tag="RD477_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632885.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            34472..35050
                     /locus_tag="RD477_000495"
     CDS             34472..35050
                     /locus_tag="RD477_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7632886.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            35453..36364
                     /locus_tag="RD477_000496"
     CDS             35453..36364
                     /locus_tag="RD477_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7632887.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(36620..37537)
                     /locus_tag="RD477_000497"
     CDS             complement(36620..37537)
                     /locus_tag="RD477_000497"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDT7632888.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            37739..38176
                     /gene="zapA"
                     /locus_tag="RD477_000498"
     CDS             37739..38176
                     /gene="zapA"
                     /locus_tag="RD477_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDT7632889.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            38234..38782
                     /locus_tag="RD477_000499"
     CDS             38234..38782
                     /locus_tag="RD477_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDT7632890.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            38897..41257
                     /locus_tag="RD477_000500"
     CDS             38897..41257
                     /locus_tag="RD477_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDT7632891.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            41344..41658
                     /gene="trxA"
                     /locus_tag="RD477_000501"
     CDS             41344..41658
                     /gene="trxA"
                     /locus_tag="RD477_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDT7632892.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            41840..43618
                     /gene="uvrC"
                     /locus_tag="RD477_000502"
     CDS             41840..43618
                     /gene="uvrC"
                     /locus_tag="RD477_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDT7632893.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            43763..44662
                     /locus_tag="RD477_000503"
     CDS             43763..44662
                     /locus_tag="RD477_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632894.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            44655..45917
                     /locus_tag="RD477_000504"
     CDS             44655..45917
                     /locus_tag="RD477_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632895.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            46078..47976
                     /locus_tag="RD477_000505"
     CDS             46078..47976
                     /locus_tag="RD477_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDT7632896.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(48275..48994)
                     /locus_tag="RD477_000506"
     CDS             complement(48275..48994)
                     /locus_tag="RD477_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDT7632897.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            49331..49663
                     /locus_tag="RD477_000507"
     CDS             49331..49663
                     /locus_tag="RD477_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632898.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            49650..51653
                     /locus_tag="RD477_000508"
     CDS             49650..51653
                     /locus_tag="RD477_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDT7632899.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            51672..52142
                     /locus_tag="RD477_000509"
     CDS             51672..52142
                     /locus_tag="RD477_000509"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDT7632900.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            52163..52747
                     /locus_tag="RD477_000510"
     CDS             52163..52747
                     /locus_tag="RD477_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDT7632901.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            52762..53748
                     /locus_tag="RD477_000511"
     CDS             52762..53748
                     /locus_tag="RD477_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDT7632902.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            53738..54049
                     /locus_tag="RD477_000512"
     CDS             53738..54049
                     /locus_tag="RD477_000512"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDT7632903.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKKQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            54071..55852
                     /locus_tag="RD477_000513"
     CDS             54071..55852
                     /locus_tag="RD477_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDT7632904.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            55845..57221
                     /locus_tag="RD477_000514"
     CDS             55845..57221
                     /locus_tag="RD477_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDT7632905.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            57228..57863
                     /locus_tag="RD477_000515"
     CDS             57228..57863
                     /locus_tag="RD477_000515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDT7632906.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            57893..59248
                     /locus_tag="RD477_000516"
     CDS             57893..59248
                     /locus_tag="RD477_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDT7632907.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(59440..60834)
                     /locus_tag="RD477_000517"
     CDS             complement(59440..60834)
                     /locus_tag="RD477_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDT7632908.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            60926..61897
                     /locus_tag="RD477_000518"
     CDS             60926..61897
                     /locus_tag="RD477_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDT7632909.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            61894..62675
                     /locus_tag="RD477_000519"
                     /pseudo
     CDS             61894..62675
                     /locus_tag="RD477_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(62844..63215)
                     /locus_tag="RD477_000520"
     CDS             complement(62844..63215)
                     /locus_tag="RD477_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7632910.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            63349..63792
                     /locus_tag="RD477_000521"
     CDS             63349..63792
                     /locus_tag="RD477_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDT7632911.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(63835..64644)
                     /gene="yidA"
                     /locus_tag="RD477_000522"
     CDS             complement(63835..64644)
                     /gene="yidA"
                     /locus_tag="RD477_000522"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDT7632912.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(64663..66030)
                     /locus_tag="RD477_000523"
     CDS             complement(64663..66030)
                     /locus_tag="RD477_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7632913.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(66014..66844)
                     /locus_tag="RD477_000524"
     CDS             complement(66014..66844)
                     /locus_tag="RD477_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDT7632914.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            67007..67444
                     /locus_tag="RD477_000525"
     CDS             67007..67444
                     /locus_tag="RD477_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDT7632915.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            67520..68140
                     /locus_tag="RD477_000526"
     CDS             67520..68140
                     /locus_tag="RD477_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDT7632916.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            68329..68835
                     /locus_tag="RD477_000527"
     CDS             68329..68835
                     /locus_tag="RD477_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDT7632917.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            68852..69751
                     /locus_tag="RD477_000528"
     CDS             68852..69751
                     /locus_tag="RD477_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDT7632918.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKTTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            69838..70723
                     /locus_tag="RD477_000529"
                     /pseudo
     CDS             69838..70723
                     /locus_tag="RD477_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(70915..70986)
                     /locus_tag="RD477_000530"
     tRNA            complement(70915..70986)
                     /locus_tag="RD477_000530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70951..70953),aa:Arg,seq:ccg)
     gene            71046..72380
                     /gene="rpoN"
                     /locus_tag="RD477_000531"
     CDS             71046..72380
                     /gene="rpoN"
                     /locus_tag="RD477_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDT7632919.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            72529..73620
                     /locus_tag="RD477_000532"
     CDS             72529..73620
                     /locus_tag="RD477_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDT7632920.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            73661..74662
                     /gene="gap"
                     /locus_tag="RD477_000533"
     CDS             73661..74662
                     /gene="gap"
                     /locus_tag="RD477_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDT7632921.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        25047 a        12685 c        15549 g        21476 t
ORIGIN      
        1 ttttcggtct gtactgaaat caaggtatta ttgggaatcc cagcttaaat catagatacc
       61 gtaagggatt ttattcttta tttaaaactt tgcaacagaa ccaatgaaat aatagaaagt
      121 aaagtcaatg caattgtgat aaaagagtta aaggagaatt ccttaaaaag gagttttgcg
      181 gaagaaggtc taaaaataca taatgaagga gataaatgtg ctttttgtgg tggagaagta
      241 actgccgagc gtattgtaga attggagtct gttttcaata caaaggaagt ttttagatta
      301 caagatagaa taaaacaagg tattgctaaa atagaggagt gtatagatat tttaagtaat
      361 atagatatct tgaatgcctc agacttttat tctcatcttg atactgagaa ggtaaattct
      421 agattgattg atattaaaaa agagcaaatg aattttttaa aagagtgtaa aatgcaactt
      481 gagaaaaaag aaaaagagtt atttattagc atggatagtt ttgaaatgaa aattccatta
      541 tcgtttacta ctattcaaaa agatttaaat gaattgatca aaagtcataa tgaattttca
      601 aatgatatag agaaaagtaa gagtgaggcc aaaagaaaat taatactgca tcgtgttgca
      661 gaaaaatgtg aacagatggg aacggaaaga ttacaggggg aacttgcgac tttaaagaaa
      721 acaagcactg atttaaagaa aatattaagc gatgaaattg aaaaaattga atctgataag
      781 agaaagattg agtcagaaat aattgaacaa agaaaagaac ttaaacaatt gcaagaaaaa
      841 attaaaaacc cagaaataat tataaaaaac attaatgaaa aaatatcgaa atctggaaag
      901 aaaaatcttg aattgaaata tatagaatca ggtaagcatt ataaaattat taacaaagat
      961 ggttctacaa gaaatataca agaaatttct acgggtgaaa aaaacataat tgcattttta
     1021 tattttatag gaagtctgga tagccctgaa ttagaaacag gtaagcctaa aataatagtt
     1081 ttggacgatc ctatgaactc aaatgatgat accatgcaat atttaatcat ttcagaaatt
     1141 gaaaagttat atagccgaaa aaatttattt aagcatttta tcttattaac tcataattca
     1201 catttttatc ttaaagtgac ttttgggaga agagtgagaa gagatggtaa aaatccttat
     1261 gaaatggaca attttgttcg tatgataaat gatggtaatc tgacatcatt taaatacttg
     1321 aaacataaaa acgaagattt tacaacccaa tatggtagct tgtggaaaga gttgaaattt
     1381 ctttacgata acgataaaaa ggactttatg tgtaatacta ttagacgtat aatcgagaca
     1441 tatgttgtgt ttaatggcgt ctctggaaat aaagatgccg aaagtaagat gctatttaat
     1501 actaattcac actatacaga gataggggat cttgaaacgg atatcaatgg gtattctaaa
     1561 gaagatataa taggattaat gagaaagttt ttcaaacaaa ataatgctga agtacatttc
     1621 aataattatt ggaataagtt ataaaaaatt cagtatataa ttgtttatat attttgtttt
     1681 ctcatcaaaa gcacgccact tgaatacgat tagaaaacca atcaagatga attatcatcg
     1741 ataaagtgat tagctcgatt caaaaatcgt tgaaatacca actatcttca attatcttta
     1801 atcaactcaa cttcatggtt gaagcagaat aagttgaatc ctcaaataac ttctctacct
     1861 gctattttgg atacgatatt ggctacgtta ccaatgatag tacctgataa tatacactgt
     1921 cagtctatag taatttatca aatgaattat ttatacaaaa gcctattcct ttcctctgat
     1981 tcccaaaaga gtcattggaa ggatcagttg gtaacatatt ttattttgac agattcttca
     2041 gtcttttcta tcaaaagcac accacgcaac ttgaatacgt ttttgaatac gattagaaaa
     2101 ccagtcaaga tgaattatca tcaataaagt gattagctct attcagaaat catttaatta
     2161 tcaactatct tcaattatcg tcaatgaact taacttcatg gcggaagaag aagaatagac
     2221 tataaagcta ggagaaacct tgatatgata gggtttctcc tttttttatt ttttactgta
     2281 tgccatattg tatgccatct agtcaatatt taagtgttgt actaattttt caacagtctt
     2341 ttctttcgtt tcttttgcca agtgaccgta acaatcgatc gtctgactaa tttttgtatg
     2401 ccgtaatcta gtttgaactt ctaccatagt agctccagcg tccaagagca ttgaagcatg
     2461 agtgtgtctt gctacatgca caccgagaaa tggaaccata gctttttcac aaatagtaat
     2521 caatcttgca taaatagcgt ggtgtgtgat tagttcttta tagataccac aaaaaatcat
     2581 agtcggatca gttacaccaa gtgtcagcat atatttccgc tgattaatct gccatttttt
     2641 taatatagaa attgttcttt catctagtgg aagaacccca gcactacaat cagttttagg
     2701 atcactgatt ttatagccat ttttggattg gcttagtgtt ttattgatgg tgaccgtatt
     2761 ttcttgaaaa tcaatgtctg accaattcaa tgcaagggct tcactgatcc taaccccgct
     2821 ataaaagaga aagcgtagta acgttttatc aaagtcgttt cgataagttt caggtttgct
     2881 gtttaggtaa tcaaacaagg tgatcagttg ttctttactg taaactttga ccttttcttt
     2941 atgcttagtg gtggttcttt taggcattaa tacgttgtct agtggattag tctcaataat
     3001 cccaagattg atagcgtagc taaaaatacg cttagaagtt gaatgaagta ccttgtattg
     3061 gtcagtagtt tcagcccagc tatttacaat ttgttgggct ttttttaccg tgacttcatt
     3121 cacacgaaga tttccatatt ttggaagaat ccactttcta aacttgattt cagttttcat
     3181 gaaggtggct tctcggacag tggttttata tgtttctagc cacaagtcat aaacttcttt
     3241 aaacgtttcg ttgttcgctt ttttaatacc gtttttatcg tagtccactt gcaagcgtga
     3301 caaggcaagc tgggcttctt tttttgtttt gtaacctcgt ctacgaacct tatcaggttt
     3361 gccagtcatt ttgttcattc caagataggc ttcatagagc caaaatttac cttttttagt
     3421 ttcatattgc ttgaatgttg ccataatagt tttccctcca tagtgtagca agccgtgggg
     3481 gcggttggag gaggcagttt attattattt tgaatagtgt ttatttagct cagtaacaag
     3541 agctgtgaac tcttttagaa gatcattttc ataaaattct tgatactctt ttttctcatc
     3601 tgtatttttt tctgaatcat taattaatga gtcatatcgt aaaagcgtat ttagaagagc
     3661 tgcgagataa gcaattgggg tactcccgtc aggaaattca attcgagatc ctgttttaag
     3721 atattttatt gcgtttgtta aataaatctt ctgtgagaca gtcaggttat cttcttcgat
     3781 gttattgata gcattagaaa gattagaatc aattatagag ttattttttt caatagtttt
     3841 tttcaattgc ttttcaagca catttcgctt aatttcgggc atcatatgca tatctcttaa
     3901 tgtataagat ccgtttaata gataggtagt ggatacatct aatacctttg aaagttgttt
     3961 aagcctggtg ttattgggaa gattatagcc attttcccaa ttactaacgg cacctttact
     4021 ggccggtggg gataatttac ctccaagttg ttccattgag agtccctttt caattctagc
     4081 cagtctaatt ctttgtcctg tctccactgc ttcggggtat ttttttctag ccacattttc
     4141 accaccattc atatattttc tctactatat cacgatttaa atatgtgtga aacataaaag
     4201 ttcaaaaaag tatgttaaaa ttcttgcaaa tgaaaaggta tgatggtata ttgagtttat
     4261 aagaggtata gaaatgtatt caagagcctc ttgcattttt ttaaaatctt tgtatagaaa
     4321 tgtatgctaa cgagggggga taaatatgga attaaaaatt gacaaactca cgcttgatga
     4381 tccagctttt aaaattattg acgatcgagt tgttcaacaa gtagagcaaa ctcaaggaac
     4441 gttactttca ttattagaaa aaaagtacca gtttccaaaa tatatgaaca aggcacaagc
     4501 tgctaagtat atgaacgttt catacaatac tatgatgcag aaatatgttc ctaacggttt
     4561 aaagttgatt atagttgatg gagtcgtaag aatttcacaa gatgaatgtg atcgctttat
     4621 ggaagaatac caaaaataaa cgcaagccgt gggggcaaag ggattaatag gaggaaataa
     4681 taatggaatc gacacaaaat aaagcaatcg aaaaagtatt atcaacaatt cttacagaag
     4741 acacagcggc agaattagca aacttggaag gcaaagcgct agaagaaacg tttgaatgtt
     4801 tgtacgaaca aatggactat caaaaattgc taccacaaga accaacggta agcggagtat
     4861 tgcgtggctt gaacgattta gtacaagcag agtttaaaga acgactttct attgaagagt
     4921 atcaagaaat tttgtatcag caagttgagt tgttagcgag cttgctaggg cttgaactgg
     4981 aggacgttga atgaaaactg aaatttggaa cggacacatt ataagatttg tcgatattaa
     5041 tgatgaatgg tgggcagtgg cgaaagatgt tgcggaagcg ttaggtctta aacaggttac
     5101 tagagccatt cactctttgc ctaaagatgg ggttactact agtaaggtca tcgattcatt
     5161 gggcagaaca caagatgtaa acatcatcaa cgaaaaaaaa tatttaccgc atggcattca
     5221 aaagccgtaa aaaagaagcc gaggcatttc aagactggat tttcgacatc atcaaagaac
     5281 tacgccagtc cacaggactt gaaggctttc aagtattccg catgctagac aaagaacacc
     5341 aaaaagaagc aatgactaaa ttaagccacg ctattactga accaaaacca gtagactaca
     5401 ttaaagcgaa tgtgatagcc aataaagctg tatcaactat ttatggtcat tccaaaatgg
     5461 taaaaaagaa agatatgact cctgaaatgt tggttgatcg tgaacctatt ttagatgaga
     5521 cagtagaatt aatgactgtt aaagaaaaat atgggttgca gttttcagtg agtgagaaaa
     5581 tttataatcg ttctgctgaa ttgcagacaa cttaggagca ttaacatgat cgagaacaaa
     5641 gaaaaagcct tatccataac agtcttggcg gacaggataa aggcaactta attgggataa
     5701 aacccgatat tctttgttcc gattatacca tattaaaaaa tcggagggaa ccattatgaa
     5761 acgaaactta attataggaa gcattttaat cggcggaatt ttcggcttat taccattaat
     5821 tgcaaaaata gctatctttg caggattagc gttatatctc ttcactgctt atgacgagta
     5881 cgactatcaa ttaagaactg gagggcataa gtagatgtac aaaacaaaac tattaaacca
     5941 actcgatagc cttgaattag aggaaattaa tcaagggatc gctgaactag aaaataatat
     6001 cgggaaaacc tactttggaa attcattcaa tgaaaaacta acagttttgt atgtactcaa
     6061 aaaacatgca gaacacaaaa taatttgtcg agaaatcaac gagctaaaaa atcagatttt
     6121 aaccgcatgg ttaaacatta ctgatatgca agaagcaagg gtgaaaacat ttaatacatg
     6181 ggtaaagtat cagaatcaat taaaaggtgc tgaatttgtt cgggatgggt tgaaatacga
     6241 acttgagcag ttgaagctca tggaggtgtc tgaatgattt ataccagtat tttgaaaaag
     6301 gaggagaggt ttattggcaa ataattatca agcagcaacg gaattacaaa agtcgggatg
     6361 gtcaatttat cccttatctc ctggaactaa aattccaata aaaggctcta gtggttggaa
     6421 tgatgcaact aacgattcta atcaaattaa tgaatggtgg acggaagatg caactagaaa
     6481 tattgggtta atgcttgaag atcatcaaat ggtggtagtt gacctagatc aacattctga
     6541 aacacgaaac ggcgttgaga attataaaaa acttgcttca atgtatgatc catttccttc
     6601 tacttatacg gaaatcacac caggtaaagg aatccatttc ttctttaaaa ttcctgatgg
     6661 tgtaattatc caacaagaaa caggtgcatt tgctgatatc ttcgggcgtg atgataaagg
     6721 taaaagttta agcgggattg atattatcac taaaggagtt ccaatagcgc caactatgat
     6781 tatttcaaga agtgttggga gagcatatga acctattgaa aacgtatctc taaataatat
     6841 tgcaccagct cccgattggc tagtgaacct actgctaaaa aaaccgcaag aaataaagac
     6901 tcaataccga cccgtagcaa aaacagggac agcaaaaaaa ttagatatga ttgttcaagg
     6961 cgctggcgaa ggtagtagaa acgatcattt aactaagtta tgcggttggt tactatggca
     7021 cggtgtagat aatcaaactt tagcagaatt gatttataca gcaaatgcgt acaatgaacc
     7081 accgctaaaa gataaagaag tgaatcaaat tattcgatcg atgattaaaa aagatttgag
     7141 ggggaaagct catggctcat aagcataaat tagatagtat tttagacttt cccgaagcca
     7201 gcgagcgtga agacaatatc attgaattga aaacctggat gtcacgatta agatgcaata
     7261 aagacgatca gattaaaagt aacagtgttg tgaatgcaga attgatttta accaatgata
     7321 gcaatttagc tggaacaatt gcttataacg aatttagcgg atacattcac ctattaaaag
     7381 attctccttg gatcaatcgt accgctggag aatgggaaga cagttttgag gatgccttaa
     7441 cggcatatat tgaagaaaac tacaatgtag tttttgatga caacaaaata cacaaagcag
     7501 tagtaaatgt ggctagaaag aatgttttca acccagtaaa agaacgtatc gaaaaagtta
     7561 aatgggatca acagcctaga ctggaaacac tgtttattga ccttttaggg gtagacgaca
     7621 acctttacac acgagaagta acaaaacgtt ggatagtcgg atcagtcgca cgtatctata
     7681 aaccaggaat aaagtttgag atcgttccag ttttggatgg accgcaaggg attggtaaga
     7741 gtaccgttcc agcattactt tacacggatg attttttcac cgattcatta gattcgctag
     7801 gtgaaaagaa agatgattat atgcagcttc aaggcaatgt aattattgaa cttggagaat
     7861 tatcttcaat gaacaaaact aaaattgaac agataaaaaa cttcatcagt gccaagatcg
     7921 ataaaattag acctccttat ggtcggaatg tcatagcttg ggcaaggcaa tgtgtattta
     7981 ttggaacgtc gaatgatggg cagtatttga aagatgatac aggaaaccgc cgtttttatc
     8041 ctttgccttg taagaataag ccaaagatga atccgtttaa aacaaaggat gaatattttc
     8101 ttcaagtgtt agctgaagca aaggtgcttt ttgataaagg acaacgaatc tattttagtc
     8161 ccgatgaaga taaagaagtc ttagaaattg caaaagacta tcaagaggat gccaagatgg
     8221 agaatccaat taaagaagca atcactaaat atttggaaat ggaaatcccg tataaatggg
     8281 aagaagctcc agcgtgggcg agacgttctt attatcagaa ctatcctgac agtaaatgta
     8341 atgagaaaaa tttacagcat ttctataatg gagctactat gcaacaagag ttttatttag
     8401 ttgatagcgt actcaccgct gatattttgg aagctgtttt tgataaacaa gcaaaggatt
     8461 tattgaatgg tcgatcagat gcagaaacta aaaagattgc cttgattatt agcagtattc
     8521 ctggttggga acggaagcaa ctgccaaaaa gaaataaacg acgaggattt tacaataagt
     8581 caaatgccta tgaaaataaa aatcgagctg gaaagtacaa aaagtaacgg gtagtaacgg
     8641 gtcagtaaca cctagcccgt tactagttta cccttactgc cccaagggat acagcaattt
     8701 ggtaacgggg taacacctta tttccttaaa acttttgaaa tagttagcta atatgctggg
     8761 gaataatgtt actggatttt acccgttacc tgttaccaaa acgctagaac ccttgtcccc
     8821 gttgggattg agtggtaacg ggtacctgtt acgtaggtgt taccacgtgt tacgaatcaa
     8881 ggaggaaagt aaatgctcaa tattatttca acaaacaaag caccgaactt tcaatataca
     8941 gatgaaatgg atcggtttct aatgaacacg ctggcattta gtgtgggatt ggtaacagaa
     9001 gattattcca catttgatcc agaagtatta aagataatgg aagaagaacc tgattggctg
     9061 caggagtcag tagcgtggtg tcaatcgcta gtagtgggat cattggtaga tagtggcaac
     9121 tatgatgata caggtgagct aatggatgaa tttaattgtc tgcttaacct atatgatcga
     9181 gcgagacaac gagagcttac atcgaatgag gataatttgt ttttaaacat ccatgataag
     9241 ttcttggctt tgctactaac agatgatgaa ttgataacta atttattgga ggtagaataa
     9301 ttatgcatat ggaaagtatt agatacttcg atggtgagaa acacattgag tattcaaaca
     9361 ctcaagaaga tgtagacttt attagttttg aggaagataa aatcactaaa gttaatttta
     9421 aagacggaac gtgcttgaag atagtttcgc cttatattga atacaaatca aaatgggaac
     9481 aagatgattc tgacgaacga gatcgctttg gttggtaatc tatggcagtt aaaaaacaat
     9541 gtaatcatgc tggatgtaaa acattgattg attataggca gaagtattgt gggaagcata
     9601 aagcaaagca aacggcaatt aagcgtgaag aaagaaagca aagcgaaggt aaatattttc
     9661 aattctatca aagtagaaca tggcgaaagg cttcatactt gtatcggtta aatcatccag
     9721 tatgtgagga ttgcctagaa gaaggcttga taaggaaagc tgatgttgta gatcacaaaa
     9781 ttgaactaaa agatgattgg aatagacggt tagatgaaac taattttcgt tctttatgcc
     9841 atgcacacca taattccaag acagccaatg aaagacaaag acgagaaaag agtacccttt
     9901 gagttaaggg ggcatagtgt gaaacttact gacaatcgat gcctactcat tttggtacaa
     9961 ataaccgttg tgaaaaggca taagggtaat tacaaataac gattacactt gtaatcacaa
    10021 gtgaaaaagt atataatgaa agtaggagat ttattgaaaa atacaattgt tattactgat
    10081 aaaacaggga aacagcgcac cattgagtta cctcattttg gtcaagtgac cattcaaatg
    10141 cagaacggaa aaatcattta tatagataaa ttagataaag aaaaattctg atcgaaaaac
    10201 gaaggaatgt gagcttaatt gcttacgttc ctttttcttt tgtctgaaag gaggacaaca
    10261 tgggacaccc taaaatatta cacgatacaa aaggcaatat atcaagtgaa gaaaaagcaa
    10321 tccgtgtgga cgctagagaa gaattgttca agcagcaacc attaataaat atcacgcccc
    10381 ctgactggat ggcagcgagc gctagaagtg aatggaatcg tatcgtacca acattaaaaa
    10441 aagattatcc attgagtgaa gcagattatg gttcattggt agcctattgt ttagcctttg
    10501 ctcgaatgaa aacagccgaa gccgagataa gaaaatcagg tacgtttatc acatgcgaaa
    10561 acggagtaaa gaaagccaat ccagcggttc gggttcaatc acaagctatg agtgatttga
    10621 aaaaacaagc cacctcacta ggtatgacct tagaatcacg atcaaaacta gctttgaaca
    10681 aggttaaaaa tgatgaaccc gaagacccgt tcaaagagtt gatgaattca tgaatgatta
    10741 cattgagaaa gtcctatcag gtaagctgat tgcacccaag aaaattatcc aagcgtgtga
    10801 gcgtcatata agcgatttgg agcgttctaa gtcagatagc ttcccttatg tgttcgatga
    10861 agaacaagcc accaaagcga ttaaattcat tgagttgcta ccatctacgg acggtaaagc
    10921 aatcaagatg ttaggatttc aaaaattcat tctaggcagt ctttatggct ggcgtactaa
    10981 agaagggaat tacaggcgat tcaatcgagc gtttaccagt atgagccgta agaatgggaa
    11041 aacgtatatc gcaagtggca tggctgccaa tgcgttgatt atggaacaag aaccagcaga
    11101 agcaaggcaa gtattgtttg taagtaacgc tttgaaacaa gctaaattgg gctatgatat
    11161 gctgtctaat tcacttagaa acgtggtcaa gtctagtaag tttttaagac cacaactgaa
    11221 aattatgaac tctaagattc agcacttgcc ctctaattcg ttcgctatgg cactggctag
    11281 tgaaaccagc acgctagatg ggtttgcacc aacaaccgca attcttgacg agtggcacga
    11341 agcaaaaact cgtaaaacgt acaacgtcat taagtcagga atgacccaac aaaagaacgg
    11401 cttattgtgt gttattagta ccgctgggct tgatttaaac gttcctatgt acgaggaata
    11461 cttattgtta gatcgtgtgc taaaaggcga agaacaagcc gacaggtact ttatagcgat
    11521 atgggaattg gacgatcccg aagaaattca cgatcaagag aaatggatca aagccaatcc
    11581 gatttttgaa agtgaagaaa tcaaaaaagt aatgattcca accattcaag atgatgtgaa
    11641 ccttgctttg aaacaaaata accttaattc tgtattggtg aaaaacttca atttatggag
    11701 acaagcgagt gaggacagct atatgattgc tgaagactgg caggcaaccg aagtagaacc
    11761 acaagatatt acaggcaagc ccgtttatat cggagtggat ttatctaaaa cagatgattt
    11821 aactagcgtt tcatggatcg taccgctaga taacggcgaa ctttattgtg attcacatag
    11881 ctttgtagcc accaaatatg ggcttcaaga caaagaaaag cgcgacggtt tgccttatcg
    11941 agaactagaa aaagctggtg agtgttccat tactcaatta gaaagtggaa tcgtggacta
    12001 tgaccaagta tttcagttta ttcaagattt gattcaagaa aatgatttgg aatgtatggg
    12061 gatttgttat gacccgtata acgctaattc gcttatcagt aaagctgaaa aagccaacta
    12121 cccaatgtta gaagtaagac aaggaacgat tactctaaac gttccgaccc gaacttttag
    12181 agaacaagtt tatgaaggca acgttattca caataaaaat acgattctca cccatgcagt
    12241 gaacaacgct attttaaaaa cggataacaa cggcattcag attaataaat caaagaacag
    12301 taacaaaatt gatccaatag ctgcattaat caatgcctat gtgtttgcaa tggattactt
    12361 caccacaacg gaaggagcga aagcagacaa tgaattttat acaagtgaag aattttcttt
    12421 ctaattacat tcataccgtt cttttacttc ttggattggt gtgtgtgttg gttgctatca
    12481 ccttactaac aaatgtctac tatggcttgt tagcgctggg catagtgctt attggggtag
    12541 cggtcatgct aaatacagaa cagaagggag gttaaaagat ggcatttttt aaagcgagac
    12601 aaaatacaac gggagatcct ttcctacaac atgtggtatc aatccaatcg gatgattaca
    12661 ccaccagctt tacaagcgtt cgtgcattaa gaaatagtga tgtgtttgca gccgttcgaa
    12721 ttattgctag tgatattgct tcaagtccga ttcaattggt caaaaacaat atgccacaag
    12781 ctgataatga actggtgaag ttactaaacg agaaacctaa ttcagaaatg gatggttggc
    12841 atttaaagtt tgctttggcg gtcaatatgt tgctaaacgg taatagcttt gcagagatta
    12901 aacgcaacgg tgaaaaggta gaagaaattc acttattacc taactcaagt gtaacggtta
    12961 ctcaattaga taatggtacg ttgtcttatc agattggtga taaaaaaaga cgtgtgaagt
    13021 ctagcgatat tttgcacttc aaatatttca cccaagatgg tttaacagga ttaccaccac
    13081 tttatgcgtt acgtgatgaa ctgaaaattc aacaagctgg caaccgcaca ttgcacaatt
    13141 tctttactcg tggtgtcagt gggtcaggta ttttgaaggt tcataagtca gatttagacg
    13201 gatcagcaaa gaacgcaatt cgtgaaaagt ttgaagaagc caacggttca agtagtggag
    13261 ataatgctct tagaacgatc attcttgatg aaacaatgga ctacaaaaca ttagaaataa
    13321 acaccgatgt tttgaagctt atcaattcca atgattggaa cacaaaacaa attgccaagg
    13381 cgtttggtgt accgattgag cgtttaggcg ttgaaaatga acattctagc accgttcaaa
    13441 gtaatctcca gtatatccaa agtacgctga ttcattactt taatgtgttt gtgagtgaat
    13501 tagatacgaa acttgaaacc aatatccgct ttaattccga tcagttacta gagactgatc
    13561 ctgaaacaaa agtaaaaaat gtattggatc aggtcaaagg gtcacttctc acgattaatg
    13621 aggggcggtc gaaaatgggg ctacccccca tggatggtgg cgatcgttta ctagcaagtt
    13681 tgaactttac gtatttagat acgttggaga aatatcaatt aaaagaacag gaaggagtta
    13741 caccagttga ataatgaaga agaaaaggaa aaacggctga cagaagaagc tgagctaaca
    13801 gccgattctc ccaaagtaga gaaagaaaat gaaaaacaac caacagacgg caaaactatt
    13861 tcaggctatg cgttgaaatt cgggcaaccg tcaaaagatt taggcggctt tgtagaagtg
    13921 attacacccg aagcattaaa agaggtggat ttatcaaatg tgttcttatt atatggacac
    13981 gattattcaa agccactagc tagtgtcaaa gcaggtacgt tgaaattgaa tgtcgatgat
    14041 acaggattac attttgaagc agagctaacc gacacgacct attcaaatga tgtgtatgag
    14101 aatatctcaa aaggggttgt tgattctatg tcctttgggt tcgtgcttgg attagattca
    14161 ttcgacaaaa aagaagatgg cacaattgaa cgatcaatag ataaaatcaa agcacttaat
    14221 gaaattagtg tcgtgaccgt tcccgcttat gattcatcaa atgtccaagt aaataagcgt
    14281 tcttacgaat cgtttatgag taacaaccaa gcaaaacaaa caaacaatag cttagaatcc
    14341 acttctaaag cacaaaagga gagtaataac atggaaaaaa ctttaatcga taacgaaaaa
    14401 acagaattgc gtgggtatga agaatatatc cgttcacaag gcgaagtgcg tgatggagtc
    14461 actactgtaa atgcagcggc agttgttccc gaagaagtaa tcggtgaagt ctttgatttg
    14521 aaacgttcaa attataactt agctcaatat gcaacagtaa aaacagtatc aaatggacaa
    14581 ggtaaatatc cagtagcaac taaccaacaa gcagtgttag caacaaaagc tgaacttgct
    14641 gaaattggtg atattgacgc tgaaatgttt acttcagttg attataaagt agaaactcgt
    14701 gctggtaaga ttgccttatc aaatgaggtt gtggaagatt cagcagtgaa tattgtacaa
    14761 gaggtcaaag atcagttagc gaaattggta gaaaacactg acaataagca tatcatggat
    14821 ttattaaaaa cattcactaa gaaaacggct gctacgttgg acgatttgaa acaactatac
    14881 aatgtggcat tagacccagc attaaataaa atggtaattc taaaccaaag cggatattac
    14941 cacctagata cattgaaaga ttcagatgga cgttacattt tacaacccga tgtgacagca
    15001 cctagtggta aatcattatt cggtatgcca gtagtattga ttgcagatac attgtttgcc
    15061 aatcctaaag ctggaacatt ccctatgatt atgggggata ttgcacaatc tatctttgtt
    15121 gctcgtagaa atcaagtaac gactcaatgg gaaaaattcg attactactc acaaggactt
    15181 gcagtgatcg ttcgcaacga ttataagaaa attgatcaaa atgcttcagt gtatattgag
    15241 tttacgccag ttgtaacacc aaaagtatag aaaaatattg ggcggcggtt tatcccactg
    15301 cctttttttc attaggagag ataaaatggt aattttggat agcataaaaa aaagtatgcg
    15361 gatcgatcac actattgatg atgactttat tcaacaattg attgatacag caggcgaata
    15421 tataaaaagt gctattgata gtagcgcaac ggataaagat atggataatt atcagcaatt
    15481 tgatttggcg gtgtcattac ttactcaaca ttggtatttg aaccgtcaag aagccagcag
    15541 cgaacggata ccagtaacgg tacaagcatt agtacaacaa atgagaggtg cttattatgc
    15601 caatcattaa gaatgtaaat gaattgactg agagagtcaa gtttaaaaag acaaaacggg
    15661 taaaagatga agatgggcag atggtggata gtgaagaaac tgtatttgaa tgttgggcta
    15721 atgtgcgttc acaaatgcta aaagacgtgc ttgctagtgt aggtaccatt cttgaaggaa
    15781 cgttgacgtt tattattcga tacgatcaag attatgaact aacaaacgat atgaaagtag
    15841 cttggaaaaa caagagttat aaaattattt caatcaatga aggaacggcg tttaaagatt
    15901 atacaacgat catagctaaa cagatttctt agaatgatta cacttgtaaa cgttgtgggt
    15961 atttagtata attaaggtag taaatgaagg gttagctgcc tgaattttgc aagacttagc
    16021 tagtcgaaat ctattgttgg actgaaaatt gtaggtgtgg ttgcaaacat gctggactaa
    16081 gaaagatgat agatgatgaa cgttcaattt taatacaggc atgctaggac tgattggatg
    16141 gttttggcaa gctgattgtg tagaaatcaa agaacacgtc tgttgtggac gtgttttttt
    16201 tgttataatt caaaagaggt gatttaatgg acaaagcaga gtttacaaat ttttattgga
    16261 agtattatct acatttagaa aatgagttta ttaataccac agattttgtg atgctcgatg
    16321 agaataattt caaaacattc tctattgaat atcaaaaact actactagcg attggttcgg
    16381 agtgtgagat tattttcaaa gaactttgcg gatttgatag cagtagcaat aaaagcataa
    16441 ctgattttaa agctgttatt aaatctagcg aattaatgtg tttggataat ggtgttaggg
    16501 ttatgaattc tttaacattg acttcactaa aaccttttgg agatgggtgg cctgaagata
    16561 caccagattg gtggaaaata tataacagag taaaacatgg tcgttcttca aattataagg
    16621 aagcaaattt ggaaaatgtg ttgtatgctt tagcaagtct atatttatta gaggaatatt
    16681 tgcataagaa agtaatacta gaaggtgaag tagactttat aagtccagag tcaaatctat
    16741 ttacgttatc ttgggaaaga aaacattcgt cgatgcaaaa agtgacgttt gaaaaagtgg
    16801 attcaaatta tgtagctcca ctcattgatt ttaaagaaca agaagaaatt taaaaacccc
    16861 cacggcttat tgaagttttt gtatgccata ttgtatgcca tttggtttaa atcgcttgaa
    16921 aaatagtgat agataactct aattcattta gtcataaacg ctgttttgat aacctttata
    16981 gttctttgat atgggtttct cattcatggc ggaagaagaa gaatagaaca aaagaatgaa
    17041 ttaataagat ataaaaaaag ggattgtgac aaacgtcttg tcacaatccc tttttccaaa
    17101 taaacgatgt tcaatcagct gcccctcggc attcgctcga aaaacggcga aatatgaaga
    17161 actattttgc catttgtttc tctaataatc aggtcaaaac gttgacttca cagtctcttc
    17221 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
    17281 gaagctgttt tttgaaattg ttccttgtga ctcggagctg aacggcaggc tcacgacctc
    17341 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata agttcaacca aacgaaaatt
    17401 atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    17461 cctcttttcg tttagtacta catttcctta aaaagtagaa aagctttcga agcctcgttt
    17521 gtttctttta ctgtataata taagcaaaga gtaaaaatcg ctaaaagata taaataagag
    17581 aaaggatata caagatgata tgattggtct gatttatttt gtggtgatcg ttctggcaaa
    17641 tacagttggc gctatttccg gcatgggcgg aggcgtgttg atcaaaccga ttcttgatct
    17701 tatcggtgcc cattctattg caggtatttc tttttactca acggttgcag tattcacgat
    17761 gtctattgtt tcgacagtgc gacaagtatc aagtgggaaa tcgctgaatt ggcagattgt
    17821 cggttgggta tctggtggag ctgtagttgg aggaatagcc ggtaacattg tattcgaagt
    17881 ttttttacgg ttgtttgaaa atgaaaagca tgtgcagctg atccaaattt tcttgacggt
    17941 attgaccttg gtttttgcgt tcttctacac caagcatcat cagccaaaat ttcatctaac
    18001 gtcatggatt tggtatctta tatgtggggg gatcttgggc tttttagcta gctttttagg
    18061 tatcggtggt ggacccatca atgtttcatt actgatgctg atgtttgcct tgccaataaa
    18121 agaagcaacg ctgtattctc tatctacgat tttcttttct caactggcta aattagtaac
    18181 gattgcttta acaagtggct ttatgcgctt tgatttatct atgttgtttt atattatccc
    18241 cgctgctgtt attggaggtt tgtggggagc aaaatttagc aggattttgt ctccaaaaaa
    18301 agtgaccttt atttttcaag taattgtcct cgtggttctt ttggtcaatt gttacaatgc
    18361 ctttgtcatc ttacaaacat taggagaatc tagactaacg caagtcaaaa tcactgaaac
    18421 atattgttct aaatgaaaaa aagagtatac tatgataaaa aaattcattt taaaaaatca
    18481 gaaattggct ggtgatttgg atgttacggg aaatgcgttt agaagatgca gaagcactac
    18541 gtgaaatcaa tgcggatcaa ttagggtatg atataccatt aaccgttacg gtgagacaaa
    18601 tgaagaaact gctgaaggag ccagagaaaa attttttctt ggtttatgaa gatcctactt
    18661 ctaaaaaagc agctggctat gtacaagcgg agtcctatga atcgattttt agcgatccaa
    18721 tgttcaatat tatggcactt gctgtgtcta aatatgctga aaaaagaggc atcggaaagg
    18781 ctttgatgac tggcgtagaa gaagaagcga aaaaacgagg aataacgaca ataaggctta
    18841 attctgcaga atacagagta gaagcacacc aattttatga acacatcggt tatcattccg
    18901 ataaaatgca aaaaagattt ttgaaaatcc tgtaaatctt tatcaaaaaa atacccgatc
    18961 accaagccga tacttgatga tcaggtattt ttgttttttt gactaagaaa gtaattgcat
    19021 caagaaacca gagatgatca aaacgatggc accaccaagt cgattgccca tttgagcaaa
    19081 agcaataagt tccatccgat tagctgcaga taagacggct acatttcctg ttccgcccat
    19141 cgagttgttg cataagccag cagtaatagt tgattcaatt ggatataaac caaacaaacg
    19201 accgacaaat cctgaaacaa ctgaaatcgc gataacactt gtcagacata agacaacaaa
    19261 ttgccatgtc aaagcagaag ctaatacatt cagattcagc aacgcaatac cgataccggc
    19321 aagaacagct gctgtcaagt ttttgacaat caagttattg aacatgatag ctgcatcttc
    19381 ataatactct ggtaataggt tggcgatttt acagatgaca acgataataa tcataaatgc
    19441 gtatgcatgg actttaggaa caaaatagtt accaatttgt cctaagatga agaaagtcaa
    19501 tgataccatc aaaccaacac ctaactgtgg aatggaaggt ttcacagttc gaggttttcc
    19561 atcgccaaga tcgccttttt ccattaattt accattgccg ttgaattttg gcattgattg
    19621 tccagcacga gcaactaatg ctgcaccgat gatagctaag acatttgtca ttgctgtagc
    19681 aggaatcagg cgggaaatga tagaagaagc atccgtacct aaaacatttg cataaatcgt
    19741 tgataaagga acggctccgg ctccaacacc accagccatc gcaggtaatg agatgtataa
    19801 aacggaattt gcaaaaccat ttccgataag catgcccacg agtcctacgg caaaaaagga
    19861 gacgaccatt gaaataagtg caacgggaat aaaacgaaca gaagctttca tcaatagatt
    19921 acggttcata ccaaggatgc taccagtaat caaggcagca atataaaagt ccaaaaagcc
    19981 catattgctg acaaagtttt ctgtggattt cactacatat tcaggtaaga tcccaaatgt
    20041 agcaatcgca gcagaagcga agatcgcaaa aacggaacca ccacccaaat aacttttgac
    20101 gatagggatt tttgtcccga taaaatgaaa taaattaccg aaaataacca aaactgcgat
    20161 tggtccaatc atattcgtcg gtaatttccc taaagccatt gcaatgatca ggacaaggac
    20221 catgatcaaa taaagtggca aacttacgcc ggtgattttt gtggaccaaa aaccttcttt
    20281 tttcttttcc tcaactgcag ttgcagttgt tgttttttgt gccattttta cagatccttt
    20341 cttattctgt atcatatgaa aaaagccaaa gaaaagcgac aaaagagcag gagttggctg
    20401 gccgctgttt tgccgcagtc gcttttttct ttttagacgt catacaaagt ttctgtatgt
    20461 tcttttacta taacgatctt aatcttctaa agatttatat tcaggaaccc atttagcaga
    20521 ttcaactgct tctttgatat ccgtgatttc ttcacgattc aagttttgtg cgacaactga
    20581 ttttgcgaca gtttcagcaa tcgtttgaga aaattcagtg atttttgcta ctggaggcaa
    20641 gatagctgct cctggttttg ttacatcaac gattccgcct aatgctcgac tagcttgaga
    20701 aatgatttca gcatttactc gtgtagctgt tgaagcaatc aaaccaagtc ctaaacctgg
    20761 gtacatcaaa gcattatttg cttgaccgat ttggtaagtt acaccattat attcaacatc
    20821 agcagcaggg atacctgttc caactaatgc ttttccgtcc gtccattcta ttaaatcttt
    20881 tgcttttgct tctgccaatt ttgttggatt agacaaaggc atgatgatcg gacgtggtgt
    20941 atgagcagcc atttctttca cgatttcttc agtgaatgct ccaggttggg tagaagtacc
    21001 aatcatgatt gtcggatgga tcgcttttac aactgcttct aagtttgtca attcatcagc
    21061 attagtgaaa tctgagcgtt tgcgagcgaa tgggatttga ccaggagtca atccttctgt
    21121 atcttcaaac aataaacctt gtttatctac ttgatagaaa tgttggcgtg cttcttcttc
    21181 aggtacacct tgacggatca tctcatctaa aaggatgttt gcgataccca caccagcagt
    21241 accagcacca aatgtcaaaa ttatttgatc tttcaattgt tcaccggata tattcaatcc
    21301 gcctaataca cctgctagga caacaattcc tgtaccttgg atatcgtcgt tgaaagtagt
    21361 gatttgatct tcgtattttt ctaagatcgc tgcagcattt cctcgtccaa aatcttccca
    21421 atgcaataac aattctggga ataattcggt agctgattga acgaattgat cgatgaattc
    21481 atagtaagtt tcgccttcta cacgagcatg acgattgcct aagtataagg gattattcaa
    21541 taattcttca ttattagtac cagcgtcgat cgatacaggc aaaacttgtg ctggattaat
    21601 accagcagca gcagtataga ccatcaattt gccgatagcg atgtcaacac cgttcacacc
    21661 ccagtcgccc atacctaaga ttccttctgc atcagtcaca acaatcaaac gaatgtctcg
    21721 tccatctgcc gcgttcttca aacttgcttt gatgttttct ggttcgtcga ttgaaagaaa
    21781 tgcagcatct tgcggtttca agaaaatttc gttgtattgt tcgattgaat ccgcaacgac
    21841 aggatcataa acgacaggca tgaattcaac taaatgttcg cccatcaatt tataaaacaa
    21901 tgtccgattc gtattgaata gattcattaa gaagatacgt ttttccaaat ctgtttgttt
    21961 gcttaagtat tgaccataag cttgaacaga ttgttgttcc agtgtttgga cagtacttgg
    22021 aagcatacca gtgattccat attttgcccg ttcttctttt gtaaaagctg ttcctttatt
    22081 tagaaaagga ttatttaata agtttaaccc tgtaagcatg aatgattcct cctaagtttt
    22141 aatacaaacg aagtatagaa gtcagatgat gatatagtca aaaagagcag gtatgataaa
    22201 ttttatttat atgtaatata gccaaaagaa atcaaaatag cagtatttgt tctttttata
    22261 tcgacaatcc ttgcttgaat actatgcgta tctcttatat gctagagaaa gaaaagtaga
    22321 agaataaggg atagatggaa tggataaata ggatttctga ggaaaaaagc acatgatagc
    22381 gttgatagga gtcacctatg tcaggtgata aaaatacata agtagaaaat taagaggagg
    22441 aagagaaaat gaaagaagtt caattgatta gaaaaagcga actatcagaa ggcggttgca
    22501 atgcatgtgg cgtagtagaa gcgacaagct atacattaaa attagattcc aatcaagcaa
    22561 taatctctga actgacagta ggcggtcttg tggacagtct ggcgcttgct gaaggtttta
    22621 ctggagaaga tatttatgag atgttttcag aagtccgtca gttaaaaaaa ggagaaaaat
    22681 gcatagaggt tcatcatgag agcccgaatg tgaggttcaa acgtggtgat aatgagatga
    22741 tcttcaagaa tcatgtgtct gatcacactg aactatatga aatcgtgaat caaatactgt
    22801 ctgggatttt tgaattagga ccttatgagt tcaaggaaga aaacggtaat ccaaaattaa
    22861 atgaagaatg gcaagaaacg attgaaacac aaagaaacaa tccacactta tttcaatagt
    22921 cttccacagt atttcgtaaa aatgttgatg aaaaagaaac accgtgaatc agaggtcgtg
    22981 aaaaacctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcctatttt
    23041 tcgtttggtt gggcttatta ccgcagatgc aagtttttga acaccgttta aggaagagac
    23101 tgtgaagtca acgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    23161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt ttatcgagag
    23221 gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
    23281 catttttgca taaatttgac ttaataccga gaaaatggct tttgaacacc gtttattcaa
    23341 aaaaagggac tttgacacta cttttgtcaa agtcccatcg gttttttatc tatttttatt
    23401 acaatcattt cttccagagt agtgacatct ggaatggctg aaatgtaaac agattatttg
    23461 cctgtatcaa tgtcgaagag taatataaaa agtgtaaacg ctatcatatt gttcatcatt
    23521 caaattttat agagagaagg cattgagatg gaaaaaacat atcaagcagg tacaaatgaa
    23581 ggaatagtag attttatcaa tatggaagat ttagaaatcg cggcttcaca agtgattcca
    23641 gctggtggtt atggttacat cagcagtggt gctggcgact tatttactta tcaagaaaat
    23701 gaacgggcat tcaatcatcg tttgattatt cctcatgtat taagagatgt agaattaccc
    23761 gatacaacta cacattttga cgaagaaatg ctgactgcac caatcatcat ggcaccagtt
    23821 gctgctcatg gattagctca tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat
    23881 tttgggacga tctatacagc aagttcttac gcttcttgta ctttagaaga gataagagaa
    23941 gcaggcgggg agaaagcacc gcaatggttt caattttata tgagcaaaga taatggaatc
    24001 aatttggata ttcttgaagt ggccaagcgt aatggagcaa aagcaatcgt attgacagcg
    24061 gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa acgggtttac cttcccgctg
    24121 ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc aaacgatgga tgctgtatat
    24181 aaatcttcca aacaaaaact tagtccaaaa gatgtagaat ttatcgctgc tcattctgat
    24241 ttgccagtct acgtgaaagg tgttcaatca gaagaagatg tctatcgatc attagaatct
    24301 ggtgctggtg gaatttgggt atctaaccat ggtggccgtc aattagatgg ggggcctgct
    24361 gcgtttgatt cgttgcaata tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt
    24421 gacagcggtg tacgacgtgg acaacatgtt ttcaaagcaa tcgcatcagg tgccgatctt
    24481 gttgctattg gcagacccgt tatttatgga ttgtctttag gaggaagtac aggagtccat
    24541 caagtgtttg atttctttaa aacagaattg gaaatggtca tgcaattagc tggaacacaa
    24601 acagtagaag atatcaaaaa aataaaattg cgtgagaatc gttttattta agaaataaat
    24661 gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc tatttttata gttcaaggat
    24721 gtcactaata gaataatttg aaacgaaaaa ataaaattga aatatttttt catacttaat
    24781 aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt tttctctttt atacaaaaag
    24841 ggtcataatt tcaagtaagc ggatgaattc atgaggattt tttgcatact gtctctacta
    24901 tacttaggtc actaagaaaa aggagtgatt tattatgaaa aagaacatga ttgggacatt
    24961 attactttca acacttcttc taggcggctt ggcaacacca gcttttgctg agggtcaagc
    25021 gacttcaaaa ggagatatta cctttactga accaacaaat accgttgagc ctcttaaccc
    25081 tactgatcct tcaaaaccag tagagccagc agatccggaa aatccagcaa ctggccagac
    25141 aggttcttta acattggatg tagttcctga attgccattt ggtacacacg agattgaaag
    25201 cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat acaccttatc tccaagtatc
    25261 agatcgtcga ggtgtaggtg cagatggcca agcacaaggg tggaacgtga cagtttcggt
    25321 ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct gaattagatt ttggaacatc
    25381 cactgttaaa agtacgtcag ataacgaatc cactggacca actagtcaaa ctgtaactgg
    25441 gttgagtaaa gcaagtgcag cgacaccaat atttacagca gcaaaagatc aaggtctggg
    25501 aacatggctt tctgtttatg atccagcaaa tatcacactg aaagtgccga aagcagcagc
    25561 tggaacattc acggctgatt tgacttggaa cctcgtggct ggcccagtag catagagtat
    25621 gaaataaatt caacaaactg cggtgaaaat actataggtg aaggattttt taccgcagtt
    25681 gttgttgaaa ggatgtggtg gacaatgaat caaagtaaac gactgtttgt ttcgtttttt
    25741 tctattttaa gtctattttt catttttccg tcaatcagcc aagcaaagga ttcagcagga
    25801 gactttggaa tcaaaccggt atttccagag aaccaaatag ataaagcaat cggttatttt
    25861 gatttattag tggcaccgga acaaaatcag acactagaag taattatcag caattcttca
    25921 gatgaagaac gaacatttga ggtctctgtc aatccggcgg taacgagtga tggtggaacg
    25981 atcgattata gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga tgtccgtgac
    26041 gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac aactatacct
    26101 atagaagtga aaatcccagc aaaaagcttt aagggacgtg tattagcggg tattcatgtt
    26161 tcacctaaag aagaagctga aacagaaaat gctacagaag gagcacaaat caaaaatcgt
    26221 attgcttata atctcgcagt cgttttacaa gaaagtcaag agacgataga accggatctg
    26281 aaacttttat caggagattt ggacgaggtc aacgcaaagc caactgttca gcttcgtttc
    26341 caaaatcctc agcctaggat tatcagcaat ttgatattta cgagcaagat cttttatgaa
    26401 aatcagcttt atattgaaaa tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt
    26461 cacttgaatc ttgatttagc tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc
    26521 atagcaaaat caggcgacag taatgaatgg cgatttacac aaaactttac gatcaaaaaa
    26581 gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca atctttccca
    26641 tggctttatg caggattggt tgctgcagtt ctagtgttat ggttgatttt gctgtttttg
    26701 tgggtccaca agaaaaagaa aaaaggagaa acaacatgat gaggtgattt cgttgaaaac
    26761 atcgatctta ttgggaggga ttattgcaag tttgctgttc ttcagctggc aagagccagt
    26821 ccaagcagat accaaaagtt caggtacgat tacattttat gatctaagtc cagttccgca
    26881 tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca cagctaccga tttataaaga
    26941 atctaatcaa catggcgtat tcccggagac gaatgaagaa aagatccgtt ttctttattt
    27001 gatcattggt atagaattat ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa
    27061 ggaggatgaa catgttgcta caaaataaat tttcattttt tcgctccttc ttttttactt
    27121 cgctttttgt tttaagtata tttatctttt caccttcggc agaagctgtt ggagcaacgc
    27181 ctcctgccat ttcttatgat ccatacacgt atgaaaatat cgtacctcca aatggtggac
    27241 gtagtgcagt caatggcggg aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat
    27301 tgactgctac ttatttgaac gatggttcaa atgatccttg gaaaggtggc acggtccgtt
    27361 ttcattggga aagccatgtg aaactaacaa atgtacttga tttggtgaat atcactgcac
    27421 aaggatttgc atttgtggcg atcatcaatt tgccggaaca tgtatcaagc agtgatgtat
    27481 taaaagcaat tgactggaac aacgcctact tgtatattgc cggtgccccc atcaaggtta
    27541 ctagcggtag tttattatca gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg
    27601 atacaaatac tctattggca gatattattg ctattattac gcgaggcgaa ttcaacgtgg
    27661 acgatatccc gcttcaattt gatgttacag ctaatgtcgc gaagatgaca gaaaacggag
    27721 ataaatatga tacgagtcca aacaaaattt tgaccaaggg taaattacaa ccttctatgt
    27781 ctaaaaaggc cgttttttct gttgattttt atgattcgaa taatattatc caagatcgta
    27841 tgttcaattc gattttagct ccagctgcag gtagatttta tcctaaatta actggcggaa
    27901 acttgaatcc aggagcatat gcattttctg caagtgcaga gataaatacg tggaatagtt
    27961 acatctcaac aagtgattca acaggaaaat ataatacatt tgctgatacc acagaatacg
    28021 tggatggaaa taaccagaac cttccaggct ctaccaatag tcggacattg acgatggggc
    28081 tgactgaagg acgttttgat cagttgccat ttagtcgctt tgatcgcgta gtcaactatt
    28141 ttacaggaaa aaacgttaca caaggagcaa tactgactca tactccagca agcacaagtg
    28201 gattgaatca gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt
    28261 ctcctgttgc tttaaaggta ttggagcagt acaagctaga cggaacggtt ttaccaacaa
    28321 acttaacagc cgatagtcag tggggaaagc tcctttcttc tgcatcgtac caagtcacgg
    28381 ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag
    28441 gaacccaaaa attagcgaca ggatggacag atcaactttt ccaaacgatc caatcaaata
    28501 acgggggtgt aaatacagct acttctacac tacctgcatt ggcttcaggg caatattatt
    28561 ttgattatcg cttggtggac gatgtactta ctaacatgta tccgtctttt gcgtacaaat
    28621 ggcagtctga acaagctgga atcaccactc ttcctcaaat aacagtggct aattttccat
    28681 ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca acctgagaca ggagatcccc
    28741 tgtctgctct atcaggagat acgattcaag aaaatgtgac attcacgtta acaaaatctg
    28801 gggatgcgat aaccgacaaa aaaatcacga tctcacttcc tgaaaatact acttatatca
    28861 atggttcctt aaaattaaat ggtacaacta tcgcagatac cggcattcaa caaggagttt
    28921 ctgtcccagc agatctattg agcaaaatag gagataccat tcatcttact tacaactatc
    28981 aattaaatac tgtagatact tctgtacaat ccgtcagcat actgacaaag gcagctgtac
    29041 tttccagcaa tattacatta gcagatggtg ctaagcttcc taacccagtt gtccaaacat
    29101 ctgctaaaac aattcttgtc cccaaacaag aattaacatt agttaatgta ccagatgatt
    29161 ttacatttgg taacgatctc cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg
    29221 atttttcttt tgatgtgcgg gatactcggc ttccaagcac gtctccttgg caactgacag
    29281 ggacattgac ttctttattt aagaatgatc aaggtcaaga attaagcgga acaaaattat
    29341 acttcaatca ttctggatca aaacaattga ttcagcaagg tcaaaacacg ttgatttatg
    29401 agagtgatgg aacagctaaa ggagaggttc tcgttgactt tcccgatacc gatggtttac
    29461 tcttagaggt aaatagcagt acgaatgctc aacctggcgc aacgtatcaa ggcatggtca
    29521 catgggaact gacagctggg ccaaccagct aagtgattag aatttgtatg gatctgtgat
    29581 aactgtcaca gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa
    29641 atgcttggag gagtggactt ggaacttttc agtttgcttg aaaaaaatga acaacggcaa
    29701 atcaaacttt tccaactttt attagaggaa aaaggagaga tttccgttaa taacgtactt
    29761 acccgcttat cagttgatcg ggctaccttg aaagaagatc tgttttattt acaagctaac
    29821 ttggaaaata tcaaacaatt ggaaattgtt atccaagaag ggaatatttt attaatcaga
    29881 catggaaata tagcagcagt cgatgtttat tatcattgct acttgcgcta ttctacaaaa
    29941 tatcagatac ttctttatct gttggaaaat ggctcatttg atcggcagaa acttgtcatg
    30001 gaacttaata tcagttcggc tactttgtct cgcagagtga aggaattaaa tgattcttta
    30061 aatgagtttc atctgaaaat aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt
    30121 tatttttatt tccaattatt ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac
    30181 agtgatttat caggaaacac ttttttagat ctgctgcaaa aagagtttag ttttactatt
    30241 acagaagaag gacgattgaa atttgaaata tggctttcca ttatgaagaa gcgaatgcgc
    30301 tgtacgaaaa aatccaaacc agattatagc aatcttcctg ttggattgtt tcaaacgaca
    30361 caatttttat tggacctcca tacatttttg acccgttact tctttcaata tgcctttcct
    30421 tggaatcaaa atgaaacgtt ggttttttat cttttcatga tcagtaactt tacattagat
    30481 acacataact cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga cgacgtagta
    30541 gctcaattga atcgaacgtt taaacaatcg atagaagaat atttctcttt ttatgttttt
    30601 ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc tcattttgcc
    30661 tattttagag gatggatgaa tatttttggc caagaaggat taaaacagcg gttaactgaa
    30721 gtagaagata aacaattcct ggttttatgt gaaagattgg tcagacaaac atttgctatt
    30781 gttgatttta ctgaagtaga cgaggaaaat gcccgctttg aattatttgc tcgctatgca
    30841 tctattttgc ggacgctgat caagcagtta gaattacgtc tgcaaattgc ttatgatctt
    30901 ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca tccaggaaca attgctgaat
    30961 acgatcaaat atgatttagt cgaatacgag aaagaaaagc agtatgatat cattttgact
    31021 agccaattga aagagtaccc gtcacagaag tccgctaaag tttttgtatt cactagcaac
    31081 gaatataatt atgatttccc ctatttgaac caatttttaa aagaatgtta tttagaaaaa
    31141 ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca
    31201 tcacagctgc cctaaatcta agaaaacttc aaccgaaagc agaagtttta ctgatcgatg
    31261 aagatcataa agatggacta ggctatgttt caaatggaat caacctttat ttaaaaggaa
    31321 agatacgtag tttagctgaa gttgcccata acatgcggac tcttcaagaa agtggggcaa
    31381 aactgatcac cgaatggaga gtaactgaac ttgatccaga caaacatcag ctcatcctcg
    31441 catccaaaga aggaaaaaac gagaccataa cctatgataa attaatcgta gcaacaggtt
    31501 cctctcctgt caccttgtat aaacaaattg aagctgaaaa tgtctacacc tataaaaatc
    31561 tagtgcaatc taagcaagta cttgccgctt taaaagaagc aaaagaagta gtgatcttcg
    31621 gtgctggtta tattggattg gaattagcag atgctttgag aaataaagga tacattattc
    31681 atctagttga ttatatgccg aatgttttat caagatattt cgataaggat atgatcaaca
    31741 gttttcaaaa tcagctgcaa actaagcaaa tcaattttta tccaaatgaa ttcttgatcg
    31801 attggaaaaa atcagaagag aaagtagtat ccgtacaact tctatctcaa gcaataaagg
    31861 cagatatggt cattttttct gcccaaactc gtcctaatac gacattgtta aaagaaaaag
    31921 tggctttgta tgaggatgag actgtgatag tcaatgaata tctgcagaca agtgatccgg
    31981 acatctatgc gattggagat atcgtcccag tcagttttga caaaaataaa cggcatctct
    32041 ttttaccttt agttactcga gctgtacata tggcaagagc agtagcgcta actttgagtg
    32101 gtcagccaac cgcttacgac ctcagacaaa agataacagc agcagtcatt atagactact
    32161 ttttaggaac tgtagggctg acagaagatg aagcgccatt cttggaacaa aataccggtt
    32221 cgtgttcagg agaatttgat ctatttccac aatacgatga agaaaacaaa acagtaaatg
    32281 caaagctgat ctaccatccg gatacattag aaataattgg cggacagctg ataagccaag
    32341 aattcttatt atcagatttg aacctgcttg cggatatcgt gaaacacaaa actacgattc
    32401 cacagctagc agttgaggat tttggatttc tgtctgaaca cacctctcgt tttcactacc
    32461 tcaatgagtt agcttttaaa gtactagcta agagagcaaa tagcaataga gtagataaaa
    32521 gactgtgaga aatatcacgg tcttttattg tgtggggata taataaatat taatcaatcg
    32581 agaataaaaa attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa
    32641 aaatttatca agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat
    32701 gtttttagca ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt
    32761 gatgtttttg atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca
    32821 agataatcga acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc
    32881 aaattcaaat ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg
    32941 gaaagtggcg tatcttgccc agacacctgg agtcgtgact agtaaagaca gtacagaaga
    33001 aggaaaaatc aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct
    33061 tgttgaagga aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga
    33121 agaatatggt ctagccgtag gagatacagt taagttatct ggtagtgaca agacgtttaa
    33181 actaactggc tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat
    33241 caatgcttac caagagatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa
    33301 cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat
    33361 ttctaaattc atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat
    33421 gatcggattt ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac
    33481 gatgcaaaag attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat
    33541 tgcgcgttct gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct
    33601 attaggaaca gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg
    33661 gctgtttttt ggcgtcatta gcttgttgat gctggtagta gctgtgttag gcgcactatt
    33721 ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag
    33781 agcatgaaag caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta
    33841 gttaaagcat tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc
    33901 ggtccttctg gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct
    33961 agcagtggct ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagtgaacgc
    34021 gttaaactac gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt
    34081 ctgacagtaa aaaagcagtt attattagta gataagatca aacatgaaca tcgtcaaaaa
    34141 gaagcaaaag aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa
    34201 gaactatctg gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg
    34261 tccattattc tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta
    34321 gtcgatatct tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac
    34381 gatacacggc tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa
    34441 gaaaaagaat aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca
    34501 gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc
    34561 aacaagagag atcgcacaaa aagttgggat cacccaaccc aatctctatc attatttcgg
    34621 tgataaagaa aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc
    34681 tttgagagaa atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc
    34741 gcagtttttg attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag
    34801 caatctttca aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga
    34861 accttttgaa gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga
    34921 aatcgcagca aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc
    34981 tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg
    35041 agaagcgtaa agctgttata tagggagaaa aatagttagg tgaagaagca agatatgacg
    35101 aagatcgtta tatcttgttt tttttatata ggtagttttt cataaataca aatttgttat
    35161 tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag gaagtgtttt
    35221 aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta ttgggtagat
    35281 gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa aaaaatcatt
    35341 ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt gcgtacttaa
    35401 gctgatttta agttttgacc cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa
    35461 aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc aaatagatgt
    35521 ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta cggttacaaa
    35581 gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg gaaaaaatga
    35641 ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa acggcggaga
    35701 gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa atgatggcta
    35761 tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac aaacaaaacc
    35821 ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa atgatactgg
    35881 ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta cagccataaa
    35941 agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata ctacagatga
    36001 attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag atgtctttat
    36061 cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca cgacatatta
    36121 ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca ctgatctgcc
    36181 tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc
    36241 ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg aattagtaac
    36301 agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca cttattttca
    36361 gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt ggccgggctt
    36421 attatcgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgag cctgccgttt
    36481 aactccgcgt cacaaggagc aatttcaaaa aacagcttct catgtttgat gactattgtg
    36541 acttgtgacc gaggagttgg catgagaaca ccgtttattc ggaacaaggg actgtgacat
    36601 gacttttgtc acagttccat tattttttta gcagcttcaa agctttttct gtattggcaa
    36661 aatgaagttt ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg
    36721 ctacttggtc agatttcgtt ccagctcctg atggaagggt gattccagct tcagctgatg
    36781 ctttgtgtaa ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta
    36841 aatgatattg ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta
    36901 aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg
    36961 gtttttctgg ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt
    37021 tcatccgaac ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa
    37081 gcagtttata ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt
    37141 ttaactcttt tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca
    37201 cataagcagc acatactgtt agcggaccaa aatagctgcc atttccgacc tcatcactac
    37261 ctaaaacgga taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg
    37321 cttgtttatc ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa
    37381 acatcacttt tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc
    37441 tagtataagg aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa
    37501 gttggtcgtt tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca
    37561 taaaactata ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt
    37621 ttttaaagtt tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa
    37681 gaaccaactt catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat
    37741 ggcgcacgaa aaaacacgct acaaagcagt gattgccaac caaacatata ctattatcgg
    37801 acgggaaacc aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga
    37861 attgaaacaa ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa
    37921 tgcgctttcg gatcaattga agaaacaaga aagaatcttg gagctggaag aagaaacagc
    37981 agaattaaag aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga
    38041 agcaatcgag aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat
    38101 taaaaatcat gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca
    38161 aaaatcttct aattaagatc cttttatcaa aataagaaaa atttaaaaaa gttagaagag
    38221 aaaggaacgt agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc
    38281 tggagcacgt cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt
    38341 tattgctgcc cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata
    38401 cccagctgtc accccaacat caaagttagc attttttgat caggtatttg cttttcacct
    38461 agatgaagct ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct
    38521 tacccgattt gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt
    38581 agactggatt gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat
    38641 tttgcagtta cttgcctttg ttccattaga tgtggtccaa aatcaattcg gcgagcatag
    38701 tttcgctcgt tttatcgttg agagaacgcc atttcttacc aacaagatgt atgagctttg
    38761 ggtaacgaat attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag
    38821 agtagtttat ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga
    38881 aagataaggt gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa
    38941 acagatggtc agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt
    39001 accagtgagt gagaaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa
    39061 agtgcaacgt ctgcgaggcg ggatccctat acctaaactt gaaaatatcc gtccccatat
    39121 gaaacggatc gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt
    39181 attgtctacg acaagcgaat tgaatcgttt cattgatgat ctttctgaca gtgaaatcga
    39241 atttgcacgt ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg
    39301 tttaaaggaa gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa
    39361 gagcataaga cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat
    39421 cgtccgtggg aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg
    39481 ctacgttatc ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca
    39541 aagtgcttca ggacagactt tatttattga accaaaacaa gtggtggatt tgaataatcg
    39601 tttgcgtcaa taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc
    39661 tgctgaactt gttccccatc gtcaagaaat catccacaat gcttatgtga ttggtaaaat
    39721 ggatctgatg aatgcaaaag cacgatttgg taaagaagta aaagccatcg tgccgggaat
    39781 cagtgaagac aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa
    39841 agtagtttct aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc
    39901 taacacaggt gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca
    39961 agcaggatta ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt
    40021 tgcggatata ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat
    40081 gacgaacact gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga
    40141 gctcggtgca ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga
    40201 tcttggtaaa aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta
    40261 tggatacaat cgggcaaata cgattaatgc cagcatggag ttcgatgtgg atacactaag
    40321 tccaacgtat cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag
    40381 ccgattaggt cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag
    40441 ccaagattta aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga
    40501 atatctggaa atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga
    40561 agcttacagc tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc
    40621 aaacgaagtc gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa
    40681 aatgcaacag cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac
    40741 ccaattagca aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc
    40801 gaaagaacaa aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag
    40861 agggacgctt ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat
    40921 gaatgtgtcc gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt
    40981 tatccatgct gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg
    41041 aaaacgatac gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc
    41101 tggttatcct caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat
    41161 tactgattat cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca
    41221 aggcggaaat ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg
    41281 gttcgctatc caagtataga aatgctatac taatcataga ttttaaaaat ggaggtcttt
    41341 gatatgtcac aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta
    41401 ttgattgatt tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat
    41461 cagttggaac aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa
    41521 aatccagaaa ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa
    41581 gatggtcaag tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg
    41641 atcgatcaat atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag
    41701 ttccgtcctc ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta
    41761 taattacgtt gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga
    41821 atatgaaggg gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga
    41881 tcagcccggc tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc
    41941 caaaatatta aaaaatcgtg tccgctcata ttttactggc agccataata cgaaaacgga
    42001 acggcttgtt agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc
    42061 attgctctta gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa
    42121 ggatgataaa acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat
    42181 tactcgtaaa gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc
    42241 ggcgaatgag acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag
    42301 tcagacgaag gagccttgtc tttactatca tttgggacaa tgtctgtgtc cttattattt
    42361 cgacgtagat ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaagt ttttaaacgg
    42421 aggacacgaa aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat
    42481 ggagtttgaa aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac
    42541 tcgccaaaaa atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga
    42601 caaaggatgg atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga
    42661 tgtttcaatg tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca
    42721 gttttatcag gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga
    42781 caagcccagt gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa
    42841 gaaaaaatta gttgaattgg cgaataagaa tgcctcagtt gctttgaatg aacggtttga
    42901 tctgattgcc agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat
    42961 gaatatcccg accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa
    43021 tcctgtggct gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa
    43081 atataaaata aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta
    43141 tcgcagatat tcacgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga
    43201 tggaggaaaa ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga
    43261 tattcctatt gctggcatgg caaaaaacga taaacataaa acaaatgagt tgctattcgg
    43321 tatagatttg gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg
    43381 aatccaagat gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa
    43441 cagttttgct tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct
    43501 gcttaaagag ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga
    43561 aatcggactg ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg
    43621 aaagggcact ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg
    43681 tttacgtttt gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat
    43741 ttcttgaaga aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa
    43801 ctacacagct gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat
    43861 tggccaaaac ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca
    43921 agaccaagga gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat
    43981 cggttatctt ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct
    44041 ttattttgca gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgatttttg
    44101 gatggaaaaa tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg
    44161 aaatcagcaa aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt
    44221 ggatgagcca ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt
    44281 ggaattgaaa aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga
    44341 aaaaatctgc gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt
    44401 acatgagatt cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca
    44461 acaggaagta gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt
    44521 agagattacc ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt
    44581 cggctacatt cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa
    44641 agcaggtgaa atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg
    44701 ttaagtcgat ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt
    44761 atgtagcagg aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg
    44821 ataatgagca aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag
    44881 cagttgattc tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt
    44941 tagtagtctc aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag
    45001 gacaaagtac acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag
    45061 caagtcaatt aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga
    45121 ctcgacaaaa agtcaatttt gattcaaaag gaaaaatgac agttggagaa gataactcga
    45181 cagtccaata tgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt
    45241 atgcgcagat cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga
    45301 tcctgtccag tacgactgca caaaagcatt tttacggaaa actaacaggt gttttacttg
    45361 ttgcattaac gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca
    45421 aagatatgga aatggtcaaa aatatattag acggtatctc attggataac ttgattggac
    45481 cattcttgtg gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag
    45541 cagctttatg tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag
    45601 tgacttatct atctctaggc ggatatatgc ttggattgat actaggagct tcagatccaa
    45661 acaatatcgt tattcggatc acttcgtata ttccattctt atcttcatat attatgccgg
    45721 ttcgcttagc aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg
    45781 tagccgtaac attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg
    45841 tgtatagcga aggcggatta tggagttcat tgaaacaatc gatttcaatt atgagaaatg
    45901 aacgaaaaaa agcataaaat atagaaaaat atagaaggaa gctggacatt tattgttccc
    45961 agcttctttt ttgtatagac ctatttctat ttttttatta tattggatat atattcattg
    46021 aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg cgttttaatg
    46081 gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt catcacggaa
    46141 atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagcgat
    46201 atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa
    46261 gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag ccaattcact
    46321 atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa acaagataaa
    46381 tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa atacactcga
    46441 tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat
    46501 atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc
    46561 ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca aaaatctgtc
    46621 atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc agccaaagta
    46681 atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca tgatattttg
    46741 tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg gattgccgca
    46801 cgctacaact atttatttga attagagaaa gaatatggat taaatttacg tccattattt
    46861 tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa caggaaagat
    46921 ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc tatcctccaa
    46981 ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa
    47041 ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca gctctatgag
    47101 ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt cttggaagag
    47161 gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag aatgcaaaaa
    47221 cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtactg
    47281 ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga attagaccaa
    47341 tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga aagcgcaaaa
    47401 gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg gagcgggcca
    47461 ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt tctcacagag
    47521 cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga agagtggttc
    47581 agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat catcaatgga
    47641 cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg aaaagcattt
    47701 gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc tggctactca
    47761 ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt gcctattgaa
    47821 caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc
    47881 ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca gattactcaa
    47941 ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc aacattgctc
    48001 aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg aattagctca
    48061 aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact caggtcaggg
    48121 cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
    48181 cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcagagtt
    48241 gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca aagaaatcga
    48301 ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat tggacaccaa
    48361 ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg ttggaaaagg
    48421 cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag gaattcacaa
    48481 agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg acttcgtgag
    48541 ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt ggatataatg
    48601 aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt cctcggcaga
    48661 ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt tcgaattggt
    48721 ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa agttgcggac
    48781 taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg
    48841 aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa actgcatcca
    48901 caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc gctcgaatca
    48961 gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa gtagactaat
    49021 tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac ttatctgttc
    49081 ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa
    49141 tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc
    49201 aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct gcctatacta
    49261 agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct cttttttaaa
    49321 tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag cagaagaaga
    49381 aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag aagaaaaaga
    49441 gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt tggtagaaga
    49501 aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga aagcagaagc
    49561 agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc aagcaatcgg
    49621 tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa aaagtgaccc
    49681 tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga atccaaaatg
    49741 ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa aaatattttc
    49801 cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat cggctgaaag
    49861 acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa aagaaactcc
    49921 atttgaaacg tcaagaactt tccttacaag acttggaagc aaattattcc gaagaaaatt
    49981 tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa
    50041 aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag ctggatattg
    50101 ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg agtgcagcaa
    50161 attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg gaagaaatag
    50221 attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca gttgctgcta
    50281 tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat gaacaaaatc
    50341 ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag cagcaaaaaa
    50401 agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa tgggtagaag
    50461 aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc catgccactt
    50521 atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg atgcatgtgt
    50581 taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca aactcagaag
    50641 agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa ccttttgaat
    50701 tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg ccttggatga
    50761 caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac ggattgctga
    50821 tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg atgaaacgtt
    50881 tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg atctacagtt
    50941 catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc
    51001 tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc ggtctgatac
    51061 agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa gattacttga
    51121 gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt attgcattga
    51181 ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg ctatcgatca
    51241 ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg
    51301 ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat ttagtcagtt
    51361 atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca ttcaatatgc
    51421 tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg atcatcgctt
    51481 tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt gcacgattgc
    51541 agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt gacccgctga
    51601 aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa taattggagg
    51661 atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta tttgcagtat
    51721 tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt gttggaatga
    51781 caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga caggctttga
    51841 ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc tttttgattt
    51901 ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta ggcgcttctc
    51961 tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt gcagcagcag
    52021 gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt tttgctgcga
    52081 tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta ttgaatgcct
    52141 aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc agatgaatga
    52201 agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa
    52261 ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat
    52321 cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag aagtacgaca
    52381 agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg caatagctga
    52441 aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat tgtattcatt
    52501 acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt atctttctca
    52561 gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa gtgatgaaac
    52621 tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata attttctttt
    52681 cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca atcaattatt
    52741 cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat tcgcggaaga
    52801 gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa
    52861 gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga cggatatgaa
    52921 tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg gttaaaggaa
    52981 tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt ccataatctg
    53041 aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta tatcgacgat
    53101 ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc aaatgaactc
    53161 cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga gtctaccatt
    53221 cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg acggcttgcc
    53281 gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga cttgaccaat
    53341 atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat gacgacggta
    53401 ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga taaagatatg
    53461 gaagattaca cgaattttct attagagaca gattatgctg attttctgcg ccctgtcatc
    53521 cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta tctaagttca
    53581 ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc ctttttgaac
    53641 gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag aagccatttc
    53701 gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga tcggtgtagt
    53761 tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc gttatggtac
    53821 aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt atggcgtgat
    53881 ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt acaagaaaca
    53941 aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga tagggctgga
    54001 agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt atgaaaagga
    54061 gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg taatggctga
    54121 gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag gagttatcgg
    54181 agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag agacttctgg
    54241 aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg aattaggacc
    54301 agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct ttatggaaat
    54361 cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc atgagaaaaa
    54421 atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag atgttatcgg
    54481 gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg ggataaaagg
    54541 tacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat gtgtcattga
    54601 aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg
    54661 gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc gagtgattga
    54721 tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt ttggtgcagg
    54781 gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg ttgtctatgt
    54841 gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc ctgaattgat
    54901 tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta atacatcaaa
    54961 catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg cggaatattt
    55021 ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat gggcagaagc
    55081 acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt atccagctta
    55141 tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg cattaggatc
    55201 agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tcgccgtctg gaggggatat
    55261 ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg gtcttgattc
    55321 cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct attctcttta
    55381 ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg catccatggt
    55441 caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt
    55501 agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa aatccattcg
    55561 tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt caagagagaa
    55621 acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc
    55681 gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg aaaggatcgg
    55741 acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta ttggtgaaat
    55801 caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt aaagaatatc
    55861 gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct ggtgtcaaat
    55921 atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg
    55981 aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt atcaatttac
    56041 gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg gaagacatga
    56101 tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag atcctgcctg
    56161 aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat tatccagatg
    56221 agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa
    56281 aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct cagatcgcgc
    56341 gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct gctattggaa
    56401 tcacttttga agaagcagaa tttttcatgg aagattttcg tcaaacgggc gcaatcgacc
    56461 gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgagcgtatc gctactcctc
    56521 gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat gttttggtta
    56581 tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca gcacggcgag
    56641 aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct actttattcg
    56701 aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg attctaacaa
    56761 tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt acagaaggac
    56821 agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt gatgtactcc
    56881 cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga gaagaccatg
    56941 cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca aaagaattgg
    57001 ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg aagtttgctg
    57061 agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg attactgaaa
    57121 cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta aaacgaatca
    57181 aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg gcacgattga
    57241 atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca actgccacaa
    57301 gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc atcctgttga
    57361 ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg
    57421 attttgtatt ggcaaatgct tcgatcacgg agcctttcat cgaagaatta tttgttttgc
    57481 cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta aaagtacctg
    57541 tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac gggtatttga
    57601 attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt ccaaaacttt
    57661 tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc gaaaagacac
    57721 gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa acgatctatt
    57781 atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa
    57841 aaaatatggg taccactgac taagtggtat ccttttttaa aagaggaaaa cgatgattaa
    57901 acaaagcaaa caaataaaga aacgcttgcc ggctgttcag cttctcgcat taggattttt
    57961 tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta atagtgggca
    58021 tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg tgacgggatt
    58081 gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga tcatgctttt
    58141 gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg cattgataaa
    58201 gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc tagaagaagt
    58261 atcaggtgtg atgaagctca tgctttacat cctgaaattg gcctttgtga tccaactgat
    58321 tggtacggtt gctttatctt ttgtattcgt gcctgaattt ggctggttga aaggtatctg
    58381 gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt tgttaggaga
    58441 cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg ctttgatcat
    58501 ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc agaaaaagaa
    58561 gataacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat taggcggatt
    58621 cgttgtgttc tttttcacag agcgcaatgg ggaaacgcta gtggaaggga atatgtttca
    58681 acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat attattcagt
    58741 tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga tgtacatcgg
    58801 tggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg tgttgctgat
    58861 ccagatgcgt tcgatgctta aaggacgtac gagagcggaa gcatttggcc gtaccatccg
    58921 acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt tgtgtgtagt
    58981 cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca tcgaatatat
    59041 tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat tgacgccaga
    59101 cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac gagttggtgt
    59161 attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa aatacaaata
    59221 tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct agtgatttag
    59281 taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg caaaatgttc
    59341 gtttattttt tgaggcacta tccgttgttg aacctcgggt agtgcctctt tcttattgaa
    59401 aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat atttttttat
    59461 tttttctttt acgatactta gtccagaagc taaaatttca aggttattag aggataggga
    59521 aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg
    59581 gataccgtta gcaagaaagt cattctcaat tatcttagga tcaatctcag gatcaactgg
    59641 aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa gagaatcaaa
    59701 aagctgattg gctcttttcg taagacttaa tttagtttct aagatcctat ttgcttcatt
    59761 tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca catttatatt
    59821 aaaaattgct tgttctatct tgttttggac atgtactgga actacaagat aagcaactcg
    59881 caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt taggtaataa
    59941 tttttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg aatggatatc
    60001 atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct ctcgtctttt
    60061 gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga caccatgaat
    60121 cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct cgtcaaaatc
    60181 aattggaacg atctctaaat gatataactg agccaattca atgaaattag cgtatgtata
    60241 tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga tagccaatcc
    60301 attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc cttgagtttt
    60361 taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt ggtaattaag
    60421 caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa cgaatttatt
    60481 acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta cttcattggt
    60541 agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc caatattcgt
    60601 atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag taaaatttaa
    60661 atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct gtaatttatt
    60721 ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac gcttcaattt
    60781 ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag gcattttctc
    60841 cactccttaa aattgtatac catacaattt taccattgat tgtattgatt ttagaagtta
    60901 atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag tagtagcttc
    60961 ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg
    61021 taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag acaaaaatat
    61081 gtttgcgatg atgcctgctt atttggggga gaacagatat tttggaacaa aagtcattac
    61141 cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga ttttcttgtt
    61201 tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca cctggattcg
    61261 aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg cagaaacact
    61321 tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt ctaccattcg
    61381 tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata cttttatcgc
    61441 taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag tagaagatgc
    61501 agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa
    61561 agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc ctgatacaag
    61621 agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt actcagctac
    61681 cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag aggaatcttg
    61741 tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata aaggagaatc
    61801 gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt gtgcagaata
    61861 tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta cgtagttgat
    61921 gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct agaagaatgg
    61981 ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga aacagcattt
    62041 acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg agaaatcgat
    62101 ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta caaaatacct
    62161 gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag aattaaagat
    62221 tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt gactgaatat
    62281 gaagaagcaa ttggggctaa gattaaagaa gcctacttag cacgtgattt atttttcgta
    62341 ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat caaaaatttt
    62401 gaattaggaa tagagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa
    62461 ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc
    62521 ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat
    62581 caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag
    62641 cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa
    62701 attggtggag agtggcagcc ttatagctac aatggttgtt aaaaaaagac gcaaactttt
    62761 tcaatttaat gaaaagtttg cgtctttttt taattattgg tgtttgtttt ttattcaatc
    62821 aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata accatcaggg
    62881 tcaatgacaa agtagtaaga aggcgtagtg ccaggtaatc cttttaagtc agtaatctca
    62941 agacctgcag ctttatgttg ctcgtgtaat ttttctaaat catcagctgc aatagcgata
    63001 tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat
    63061 tcatagtcat ctccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca
    63121 cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga
    63181 tctttcaccc gaacacatgt gtgagccatt ttcataaatt catctctcct tttcctttta
    63241 ctcaatcata acaaaaagaa gtaagaaaat agttgatttg tgcttgttca tgagaaattt
    63301 ctcatgtaaa atggatagaa aataatactg ttaaggtgga aacaattcat ggataaacca
    63361 atttttggtg aaaaaaagca atctgctaat tatcaaaccc gttatgcagc atatattgtc
    63421 atagaaagag acaagcggat tgcggtgatc gaggctccaa atggtgcttt tttccttcct
    63481 ggtggggaaa tcgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa
    63541 gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac
    63601 tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg
    63661 aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa
    63721 gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa
    63781 aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag
    63841 tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt
    63901 tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc
    63961 aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc
    64021 aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc
    64081 gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg
    64141 cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat
    64201 gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc
    64261 gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga
    64321 atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat cttcatgagt
    64381 cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata
    64441 agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc
    64501 tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat
    64561 cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc
    64621 gatagcgact agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa
    64681 tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg
    64741 atcacgcaag gagggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc
    64801 ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc
    64861 atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata
    64921 aggcagatcg tagctagaat tcacagcagt gtaataaaca gggttatagc caaccttttc
    64981 tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga
    65041 tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga
    65101 ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc
    65161 taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct
    65221 ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa tcacttccat
    65281 accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata
    65341 atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat
    65401 cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc
    65461 ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg
    65521 tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac
    65581 tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc
    65641 ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc
    65701 atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc
    65761 tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata
    65821 aacgcctaat gaatgggtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt
    65881 gcctaattgt ttaatgcgtc gtaaacgttg gacttctttc gaattgatca aatctaagat
    65941 tactttatgc tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg
    66001 cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt
    66061 ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg
    66121 tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt
    66181 tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag
    66241 gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc
    66301 gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac atcataacag
    66361 aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta
    66421 aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg
    66481 gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt
    66541 ttgtgtcact ttgagggaaa atcaaagaaa tatttaaaat tccttcatct gatacgatcc
    66601 ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt gtttggatcc
    66661 cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt
    66721 gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta
    66781 atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt
    66841 tcattcaacc ggctcctttg tataaattca tattttctac aatattatac tctaaaatca
    66901 aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa
    66961 agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca
    67021 aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga
    67081 tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta
    67141 taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga
    67201 tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa
    67261 agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac
    67321 gaaagaactg catgttagtt tgaaagaccg tcccacagca gggaaggttc gaatcgatta
    67381 tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc
    67441 gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact
    67501 gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt
    67561 atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc
    67621 tgatttagct aaccagatcc aaaactatct tgagaaatca gacagtgaaa ttcgtgattc
    67681 actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa
    67741 tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg
    67801 aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt
    67861 tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct
    67921 gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc
    67981 tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga
    68041 cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga
    68101 ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag
    68161 gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgatt tttgtcacac
    68221 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
    68281 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
    68341 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
    68401 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
    68461 ttagatgtga tgcaagatag ttttcataaa gccttgaact attttgaaaa agaaaaagag
    68521 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
    68581 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
    68641 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
    68701 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcaactatc
    68761 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
    68821 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
    68881 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
    68941 gcgttttatt atcgaagagc aatcatttat aaaagcaagg cggagaaaga gacgtctcca
    69001 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
    69061 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
    69121 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
    69181 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
    69241 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
    69301 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
    69361 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
    69421 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacttatc tatctgtttt
    69481 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
    69541 ttggactgag caagatattc aagaagggac gatagaaaaa acaacacttg ttacgcctac
    69601 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
    69661 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
    69721 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
    69781 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
    69841 aaaagaaatt atttagtcat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
    69901 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
    69961 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
    70021 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcaatc atcacaatca
    70081 acacaagaca gtacagaagc aatccaagac ccgactgtca aaagcgtcgc aatcagcttt
    70141 gatgatggac caggagcgac cacaacgccg cagcttctgc gaatcttaaa agaaaaaaac
    70201 gtgcatgtca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
    70261 cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc
    70321 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
    70381 agtcactggc aaaacccccg cttttgttcg tccgccctat ggttctgtca cgaatgtagg
    70441 agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag attggaagac
    70501 aaggaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg ggtcaattat
    70561 tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct
    70621 gaaagagcaa ggctatcgca tcacgacagt tagcgattta ttagggcatc caacggctgt
    70681 cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac aagtatcttt
    70741 aataaataac acattttaat aagtagttga acacataact accttacttg caaaggctta
    70801 acaatatcta tacagataca acaaaaaact gtccaccaat tctctttggc ggacagtttt
    70861 cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt agaacgcgtc
    70921 ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc actacactaa
    70981 ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg ggattggaca
    71041 aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag aaactagcca
    71101 tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag gagctgttgg
    71161 cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag ccggaatggc
    71221 agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca aattacctaa
    71281 atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa gtccatttga
    71341 attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac atcgatttga
    71401 acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct acgcctatcc
    71461 aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta ggtgcacgta
    71521 gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg gaattagctt
    71581 atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa gaaatcgcac
    71641 aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta caaacgttga
    71701 gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt cctgatgtcc
    71761 gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa aatcagccaa
    71821 atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat aaagaaactg
    71881 aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg atcctccaac
    71941 gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa gctttcttta
    72001 tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact gagatagatg
    72061 tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca gatttcggtg
    72121 tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca gaacagacag
    72181 aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat caagaagaca
    72241 aaaacaagcc gttgtcggac cagaaattgg ttgaattgct gaaaaaagat gagatagcga
    72301 tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt tcatcaaaaa
    72361 gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac tttttctagt
    72421 ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg ttgagatttc
    72481 cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat gggtcgtttt
    72541 aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga tgaaataaaa
    72601 atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta taaaatttta
    72661 cgaaatatct attggatgca gccgattggg cgtcgaagtt tatcggaaag tatggggatc
    72721 acagaacgag tcttacgaac agaaacagac cttttgaaaa atctgaattt gatcgacacc
    72781 tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag ccttgaaaac
    72841 ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga atattttggt
    72901 aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt ggctgatgca
    72961 ttcggcgaag cgctgagcga agccttagac cgactacttc cagaaggcga gaatatcatc
    73021 gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa tctggaaaat
    73081 aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc gataactgta
    73141 caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca ccgcgcactt
    73201 tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga gccatctatc
    73261 caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat tgggcgtgca
    73321 ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct gaaacaagca
    73381 aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt cgtttataag
    73441 gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat catggcaatc
    73501 gcgggcggaa aatcaaaagc taaagcaatt cgagcttata tgaaaaatgc accaaaacaa
    73561 acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt aaccctttaa
    73621 aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta aagtaggtat
    73681 taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg tagatggaat
    73741 cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact tgttaaaata
    73801 cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt cattcaacgt
    73861 aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac catggggcga
    73921 actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat cagcagctga
    73981 aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg gaaacgatgt
    74041 tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa cagttatctc
    74101 aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga acgacaaatt
    74161 tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc aaatgactct
    74221 agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg caaacatcgt
    74281 tcctaactca acaggtgctg ctaaagctat cggtttagta attcccgaat tgaacggtaa
    74341 attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag aattagtaac
    74401 agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag aagcttcaaa
    74461 cgaatcttac ggatacaaca cagacgaaat cgtttcctca gatatcgttg gtatgactta
    74521 cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca aacaattagt
    74581 taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag tacgtacttt
    74641 agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat agacatgtac
    74701 atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa gtttatt
//
LOCUS       DAPBBG010000007        74440 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPBBG010000007 DAPBBG010000000
VERSION     DAPBBG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 74440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.007"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(160..444)
                     /locus_tag="RD477_000534"
     CDS             complement(160..444)
                     /locus_tag="RD477_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632922.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="RD477_000535"
     CDS             complement(462..1352)
                     /locus_tag="RD477_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDT7632923.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="RD477_000536"
     CDS             complement(1372..1590)
                     /locus_tag="RD477_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDT7632924.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="RD477_000537"
     CDS             complement(1560..3260)
                     /locus_tag="RD477_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDT7632925.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="RD477_000538"
     CDS             3527..4300
                     /locus_tag="RD477_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632926.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="RD477_000539"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="RD477_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDT7632927.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="RD477_000540"
     CDS             complement(5124..6095)
                     /locus_tag="RD477_000540"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDT7632928.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="RD477_000541"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="RD477_000541"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDT7632929.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="RD477_000542"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="RD477_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDT7632930.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="RD477_000543"
     CDS             complement(9050..9874)
                     /locus_tag="RD477_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7632931.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="RD477_000544"
     CDS             complement(9847..10527)
                     /locus_tag="RD477_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7632932.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="RD477_000545"
     CDS             complement(10550..11488)
                     /locus_tag="RD477_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDT7632933.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12596)
                     /locus_tag="RD477_000546"
     CDS             complement(11733..12596)
                     /locus_tag="RD477_000546"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDT7632934.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="RD477_000547"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="RD477_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDT7632935.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="RD477_000548"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="RD477_000548"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDT7632936.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="RD477_000549"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="RD477_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDT7632937.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="RD477_000550"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="RD477_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDT7632938.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="RD477_000551"
     CDS             complement(19462..20703)
                     /locus_tag="RD477_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDT7632939.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="RD477_000552"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="RD477_000552"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDT7632940.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22653)
                     /locus_tag="RD477_000553"
     CDS             complement(21730..22653)
                     /locus_tag="RD477_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7632941.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="RD477_000554"
     CDS             complement(23392..24678)
                     /locus_tag="RD477_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDT7632942.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="RD477_000555"
     CDS             complement(24671..25939)
                     /locus_tag="RD477_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDT7632943.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="RD477_000556"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="RD477_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDT7632944.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="RD477_000557"
     CDS             complement(26711..27157)
                     /locus_tag="RD477_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7632945.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="RD477_000558"
     CDS             complement(27250..27495)
                     /locus_tag="RD477_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDT7632946.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="RD477_000559"
     CDS             complement(27610..28188)
                     /locus_tag="RD477_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7632947.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="RD477_000560"
     CDS             complement(28265..28795)
                     /locus_tag="RD477_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDT7632948.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="RD477_000561"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="RD477_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDT7632949.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="RD477_000562"
     CDS             complement(29652..30047)
                     /locus_tag="RD477_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDT7632950.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /locus_tag="RD477_000563"
     CDS             complement(30044..31456)
                     /locus_tag="RD477_000563"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDT7632951.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="RD477_000564"
     CDS             complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="RD477_000564"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDT7632952.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(32075..32314)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32311..33771)
                     /locus_tag="RD477_000565"
     CDS             complement(32311..33771)
                     /locus_tag="RD477_000565"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDT7632953.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="RD477_000566"
     CDS             complement(33941..35098)
                     /locus_tag="RD477_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDT7632954.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="RD477_000567"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="RD477_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDT7632955.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="RD477_000568"
     CDS             complement(36565..37092)
                     /locus_tag="RD477_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDT7632956.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37322..38128
                     /gene="proB"
                     /locus_tag="RD477_000569"
     CDS             37322..38128
                     /gene="proB"
                     /locus_tag="RD477_000569"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDT7632957.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38125..39372
                     /locus_tag="RD477_000570"
     CDS             38125..39372
                     /locus_tag="RD477_000570"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDT7632958.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39448..39792
                     /locus_tag="RD477_000571"
     CDS             39448..39792
                     /locus_tag="RD477_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632959.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39909..40235)
                     /locus_tag="RD477_000572"
     CDS             complement(39909..40235)
                     /locus_tag="RD477_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632960.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40608..41809)
                     /locus_tag="RD477_000573"
                     /pseudo
     CDS             complement(40608..41809)
                     /locus_tag="RD477_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41926..42441
                     /locus_tag="RD477_000574"
     CDS             41926..42441
                     /locus_tag="RD477_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7632961.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42640..43086)
                     /locus_tag="RD477_000575"
     CDS             complement(42640..43086)
                     /locus_tag="RD477_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDT7632962.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43133..44611)
                     /gene="msr(C)"
                     /locus_tag="RD477_000576"
     CDS             complement(43133..44611)
                     /gene="msr(C)"
                     /locus_tag="RD477_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDT7632963.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44965..45594)
                     /locus_tag="RD477_000577"
     CDS             complement(44965..45594)
                     /locus_tag="RD477_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7632964.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45578..46042)
                     /locus_tag="RD477_000578"
     CDS             complement(45578..46042)
                     /locus_tag="RD477_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7632965.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46529..47797)
                     /locus_tag="RD477_000579"
     CDS             complement(46529..47797)
                     /locus_tag="RD477_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7632966.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48028..48765)
                     /locus_tag="RD477_000580"
     CDS             complement(48028..48765)
                     /locus_tag="RD477_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7632967.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48832..49401)
                     /locus_tag="RD477_000581"
     CDS             complement(48832..49401)
                     /locus_tag="RD477_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDT7632968.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49529..50401
                     /locus_tag="RD477_000582"
     CDS             49529..50401
                     /locus_tag="RD477_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7632969.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50743..51615
                     /locus_tag="RD477_000583"
     CDS             50743..51615
                     /locus_tag="RD477_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7632970.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51612..53570
                     /locus_tag="RD477_000584"
     CDS             51612..53570
                     /locus_tag="RD477_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7632971.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53821..54672)
                     /locus_tag="RD477_000585"
     CDS             complement(53821..54672)
                     /locus_tag="RD477_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7632972.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54795..55262
                     /locus_tag="RD477_000586"
     CDS             54795..55262
                     /locus_tag="RD477_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDT7632973.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55525..56007)
                     /locus_tag="RD477_000587"
     CDS             complement(55525..56007)
                     /locus_tag="RD477_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDT7632974.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56107..56970)
                     /locus_tag="RD477_000588"
     CDS             complement(56107..56970)
                     /locus_tag="RD477_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7632975.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57568..58416
                     /locus_tag="RD477_000589"
     CDS             57568..58416
                     /locus_tag="RD477_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632976.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58587..58928)
                     /locus_tag="RD477_000590"
     CDS             complement(58587..58928)
                     /locus_tag="RD477_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDT7632977.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59014..59721
                     /locus_tag="RD477_000591"
     CDS             59014..59721
                     /locus_tag="RD477_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7632978.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59708..60031
                     /locus_tag="RD477_000592"
     CDS             59708..60031
                     /locus_tag="RD477_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7632979.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60120..61157)
                     /locus_tag="RD477_000593"
     CDS             complement(60120..61157)
                     /locus_tag="RD477_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7632980.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61604..62587)
                     /locus_tag="RD477_000594"
     CDS             complement(61604..62587)
                     /locus_tag="RD477_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDT7632981.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63003..63773)
                     /locus_tag="RD477_000595"
     CDS             complement(63003..63773)
                     /locus_tag="RD477_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632982.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63766..64701)
                     /locus_tag="RD477_000596"
     CDS             complement(63766..64701)
                     /locus_tag="RD477_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7632983.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65295..66944)
                     /locus_tag="RD477_000597"
     CDS             complement(65295..66944)
                     /locus_tag="RD477_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDT7632984.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67139..68035)
                     /locus_tag="RD477_000598"
     CDS             complement(67139..68035)
                     /locus_tag="RD477_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7632985.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68028..68708)
                     /locus_tag="RD477_000599"
     CDS             complement(68028..68708)
                     /locus_tag="RD477_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7632986.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68709..69491)
                     /locus_tag="RD477_000600"
     CDS             complement(68709..69491)
                     /locus_tag="RD477_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7632987.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69484..70401)
                     /locus_tag="RD477_000601"
     CDS             complement(69484..70401)
                     /locus_tag="RD477_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7632988.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70404..70688)
                     /locus_tag="RD477_000602"
     CDS             complement(70404..70688)
                     /locus_tag="RD477_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632989.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(71389..72009)
                     /locus_tag="RD477_000603"
     CDS             complement(71389..72009)
                     /locus_tag="RD477_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632990.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(72090..72500)
                     /locus_tag="RD477_000604"
     CDS             complement(72090..72500)
                     /locus_tag="RD477_000604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632991.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            72530..73708
                     /locus_tag="RD477_000605"
                     /pseudo
     CDS             72530..73708
                     /locus_tag="RD477_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT        21513 a        15816 c        13087 g        24024 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttgt gactttttta
     4981 ttcaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    37141 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    37201 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    37261 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    37321 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    37381 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    37441 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    37501 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    37561 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    37621 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    37681 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    37741 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    37801 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    37861 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    37921 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    37981 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    38041 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    38101 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    38161 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    38221 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    38281 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    38341 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    38401 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    38461 ccactaggcg tgaccggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    38521 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    38581 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    38641 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    38701 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    38761 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    38821 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    38881 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    38941 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    39001 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    39061 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    39121 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    39181 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    39241 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    39301 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    39361 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    39421 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    39481 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    39541 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    39601 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    39661 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    39721 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    39781 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    39841 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    39901 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    39961 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    40021 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    40081 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    40141 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    40201 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    40261 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    40321 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    40381 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    40441 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaaaagct
    40501 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
    40561 gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca
    40621 tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag aacaatggca
    40681 cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat
    40741 ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt
    40801 tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa
    40861 aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat
    40921 aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac
    40981 ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca
    41041 aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca
    41101 agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta
    41161 tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt
    41221 tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa
    41281 aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt
    41341 ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata
    41401 ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca
    41461 cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg
    41521 atgataaaca atcctgtcat caatgttttt tcaaaccgat ccgactagat aagaaacctg
    41581 taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag
    41641 ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc
    41701 ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt
    41761 cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc
    41821 tattagcatc atacatgatt tattttacat gtaaatatgc acatgtaaaa cgtggaaact
    41881 ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc
    41941 tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg
    42001 atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac
    42061 cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac
    42121 ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct
    42181 acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca
    42241 gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa
    42301 aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc
    42361 cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa
    42421 atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag
    42481 caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag
    42541 gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa
    42601 aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc
    42661 ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat
    42721 cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc
    42781 catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat
    42841 agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg caattttttt
    42901 cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag
    42961 accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat
    43021 ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt
    43081 aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat
    43141 ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac
    43201 gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta
    43261 gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca
    43321 acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac
    43381 ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac
    43441 tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt
    43501 cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa
    43561 ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt
    43621 caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg
    43681 ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt
    43741 caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc
    43801 ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac
    43861 tgtctttttg tttagaggaa gacaaccgat ccggtctgat ttgttgcttt ttctttttac
    43921 tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc
    43981 gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct
    44041 tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt
    44101 cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg
    44161 taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg
    44221 ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga
    44281 tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga
    44341 aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca
    44401 ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta
    44461 ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt
    44521 gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta
    44581 gatttgttat atttactgct aaattttcca tatgttttac actcctattt catgtaaaac
    44641 aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat
    44701 cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac
    44761 ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca
    44821 tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag
    44881 tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc
    44941 tactgaatag taactgaaaa ccttttattc gaacgtagtg atcttttctg gtgctaaacg
    45001 aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata
    45061 acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg
    45121 atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga
    45181 atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat
    45241 cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt
    45301 atagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga
    45361 agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc
    45421 ctcgctttca gcgaccacaa agcgccatgg ttgcatattg acagaagaag gtgcagtcgt
    45481 cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac
    45541 gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat
    45601 taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg
    45661 ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt
    45721 tatggtccat ttcacaatgc tcgattttct tctgggcttt ttctgttagc gctacataca
    45781 cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct
    45841 tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta
    45901 gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga
    45961 tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt
    46021 gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg
    46081 acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa
    46141 aacgctctca atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat
    46201 gataaagaaa gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata
    46261 gctcttctgc agtagaaaat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga
    46321 atcccgtcga atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac
    46381 aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat
    46441 aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga
    46501 tgcagggcag ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc
    46561 tccagcccat cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac
    46621 cagttttttc atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac
    46681 ttagaccttc ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg
    46741 tgtaatgatg gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg
    46801 gtgcttgcca agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc
    46861 cggcattcaa tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag
    46921 aagttgaagt tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca
    46981 atgtagaacc ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt
    47041 tgtaagagtt cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag
    47101 aagtcgtctt caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc
    47161 taaaagcggc attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa
    47221 cagattggcc attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca
    47281 aagtactgtt cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata
    47341 agtcattggc acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt
    47401 gagaattagc agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca
    47461 aatctgcttg atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt
    47521 ctgcattctc tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt
    47581 ctgttgtttc agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact
    47641 gcaaagtact ctctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg
    47701 cgacagtttc tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta
    47761 aaagaataac gctaaataat aaattctttg ctttcatctt ttttctcctt tgaattatga
    47821 aacttttcct aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa
    47881 ataacatttg tttctttttg ataaatttgc tttttctaca taaaaagacg taaatgaaga
    47941 atattcttct ccattcacgc cttaaattga tggtcgtttg aataactatt taacaagtaa
    48001 ttctctttgg atattatccg ttatggatta tacttcctgt gtcgtcggac ggataatcat
    48061 gttactgaca gacatccggt ctggcgtttc tatcgcaaat gctacagcag aagctatatc
    48121 ttcggaggtc aatccccatt ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc
    48181 gctgatggtt tgatataatt cagtttgaac agctcctgga gagatgatcg tagatttgat
    48241 gttattttgc cgttgctctt gacgtaatcc ttccatgatt gcacggacag caaattttgt
    48301 tccgcaataa acggcggaat caggataaac aacatgtccc gccacagaat cagttgaaat
    48361 gatatgccca gatttttgtt tttccatgat tggaagtacg gcggaaattc catttaacac
    48421 acccatgatg ttgatatcaa gcatattttg ccattcatca cggtgacctt caactaatgg
    48481 agcggttggc atgattcctg cattattgta caatacatcg attcgaccga acttttccat
    48541 cgctaaatcg ataactgctt ggacttgtgc aaaatctctt acatctgctg tttgaatata
    48601 tagctcagct tctggtaaag attcttttat tgcttttagg cgatcttctc ggcgtgcagc
    48661 tataactagt ttagcccctt tttctgctaa tagcttcgtt gttgcttcac cgattccgct
    48721 tgaagcgccc atgatgacaa tcactttttc tgataaatca ttcattctat tctcctcctg
    48781 ttttgaaaaa tctcttgctt atactcacta tctcttattt catcttactg ttcaaataat
    48841 ccgttcgaca ggtttgacga ttttggcagg gattcctgca acgatggtat tgtctgggac
    48901 atctttcgta acagttgcat tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg
    48961 cagaatagtt gctcctgctc cgatccaagc atttttcttg atgtggattg gctttactga
    49021 caagcctctt cgttcttttg gatcggttag atggttcacc gtgatgaggt tgacacgagg
    49081 accaatcaat actgaatctt ctaaacagat gccacctaag tcaacgaaca tcacttgacg
    49141 attgataaag acatttttcc ctatactaat atgtttgccg aaatctgtat aaaaaggcag
    49201 cagaacagtc accgtatcat ctactttgtc actaatgata ttttctagcc gtttgcggat
    49261 atctgctggt tcatggtacc cagtattcag cgctgctaaa ggctcttcgt tttcagcttg
    49321 gatctgatgg atcacttcaa agagcggatc cgttactcga atctctttat tgacgatttg
    49381 tttttcgatg aggcttgtca ttttgctccc tccttttatc cttactttat aacggacggc
    49441 ttatcatgtc taatacttat aaaacatact taactattcc ttttaactat acttagtggt
    49501 atgctttttt aaaaacagga gggaaaaaat ggatattcgt atactgacgt attttttagc
    49561 agtagctaga gaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac
    49621 tctctcaaaa caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgggg
    49681 ctcccgagag atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat
    49741 cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga
    49801 aatctacatt ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc
    49861 gttgatcaag gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat
    49921 catagacaaa ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa
    49981 acaaaaatac cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg
    50041 aaatcatcct ttagcaaagc aaaccgtcat ccatccgcag gaactgaaaa ctgttccttt
    50101 gatcatttcc cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat
    50161 agatcagtta tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga
    50221 taacgggact tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa
    50281 aatattcatc ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga
    50341 ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta
    50401 agtagagttt ttttccttcg ctttcagctt tttaacaacg aataagataa gcaaaagatc
    50461 caacgattaa ataacataag tttcttaatt tatttcttcc ttttatttct gataattcca
    50521 gtaaattact ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga
    50581 ttacgttaac aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga
    50641 ggaacatggt ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg
    50701 tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc
    50761 tttaatcaaa atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa
    50821 gggattggat ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata
    50881 tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa
    50941 cgctttttat ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt gatggaaaag
    51001 ttgaatgaac atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt
    51061 aataacggta tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat
    51121 gaattcagta ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat
    51181 acttatgacg aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac
    51241 aataaccatc gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa
    51301 tcagaattgg aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg
    51361 aatcatttac gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg
    51421 gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc
    51481 cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg
    51541 gccatccata tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag
    51601 gaggaaaaaa attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg
    51661 cggtcttacc tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta
    51721 acgacattgt cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt
    51781 tgctgtttgc cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct
    51841 tggcaggttt ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta
    51901 ctttattgaa tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat
    51961 ttattggtat tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa
    52021 aattaccgat ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct
    52081 ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt
    52141 tagtttcttt tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct
    52201 tcttcaaccg tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt
    52261 ttgacgtagc aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg
    52321 taacaggaat gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag
    52381 catatgctat ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgc
    52441 tagcagcagc ttttgcttca ttctttactg gtgtgacaga accacttgaa ttttcattta
    52501 tgttcgttgc ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag
    52561 tgagtgcatt cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact
    52621 tcctatcttt gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag
    52681 taacagcagt gatttactac tttggcttta actttgctat caaaaaattc catctgatga
    52741 ctcctggccg cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca
    52801 ataaatatgc ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa
    52861 cttcgatcga taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg
    52921 atcaaaataa aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg
    52981 cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg
    53041 gtggtgctgc agcacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg
    53101 gtgaaacgat cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg
    53161 aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag
    53221 aaaatggaga aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac
    53281 atgctgtcgg aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca
    53341 ctgttgactt aaaaggcgaa ccattcactt tatatgtaga agaaggtcaa aaagtcgcaa
    53401 gaggtcaatt gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg
    53461 atatggtcgt tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac
    53521 gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa caaagcctga gccatcgtct
    53581 tccccagact tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc
    53641 ctttttccga ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagctaaac
    53701 gaaacatatg aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt
    53761 tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca
    53821 ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa
    53881 tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat
    53941 cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa
    54001 cgctgcatca cttgcttcaa taatttctaa ctctttacct gtcgcacgct ctaatgcctt
    54061 ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact
    54121 ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt
    54181 tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa
    54241 ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat
    54301 cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc
    54361 aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt
    54421 atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt
    54481 caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc
    54541 ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg
    54601 aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac
    54661 taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca
    54721 tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga
    54781 aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct
    54841 atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg
    54901 aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct
    54961 ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac
    55021 tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa
    55081 atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc
    55141 tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa
    55201 tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat
    55261 aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt
    55321 ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg
    55381 ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg
    55441 aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc
    55501 gctactttca cgacctctat attatcatgg tttattgact accggtatcc agatttctcc
    55561 catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc
    55621 gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa
    55681 ttcttcaggc gattctgcta aagcgcttac aacaagatac gttccttttg ggaatgggat
    55741 gattcgcgta gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt
    55801 cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc
    55861 atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc
    55921 gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg
    55981 tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc
    56041 ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt
    56101 ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc
    56161 ctctccccat tttgtaaaag agatctcggg agcttcgact aacccataat ctgaagaagg
    56221 aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt
    56281 attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg
    56341 attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg
    56401 gtctccatca aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt
    56461 atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt
    56521 gactccttca aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt
    56581 cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg
    56641 tggaaaagat ttttgtatgc cgtatctact gatttctgaa ggcagatatc ctgaccaatc
    56701 acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt
    56761 cactcttgat ccatctagaa gatcgcgatt agccaaagcc aatctacggt ttttgatata
    56821 gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat
    56881 tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta
    56941 atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgatt gcctttttat
    57001 catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga
    57061 aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat
    57121 tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac agattctaag
    57181 taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta
    57241 caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata
    57301 catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt
    57361 tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt
    57421 ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg
    57481 attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa
    57541 ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac
    57601 gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca
    57661 agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg
    57721 gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa
    57781 cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca
    57841 ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta
    57901 acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa gatgccattt
    57961 catgcaataa attattcaga taaattaaca actatcatcc ataaatacga tcatattatc
    58021 aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa
    58081 gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga attgatcaat
    58141 gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt
    58201 ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa
    58261 aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca
    58321 aaacgctact ctataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa
    58381 aagaaaaaaa tagataaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat
    58441 agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa
    58501 aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca
    58561 tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctgga
    58621 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
    58681 tccgctgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
    58741 cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc catacttaaa
    58801 caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag atttttcgcg
    58861 cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat
    58921 cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa cgacaattat
    58981 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
    59041 gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact tgcatttggt
    59101 atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc cctgctggtc
    59161 tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag cccattgctt
    59221 tccatcgttt tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
    59281 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
    59341 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
    59401 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
    59461 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
    59521 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
    59581 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
    59641 atcccaagca acttagtgat tgtagttgta acactgattg ggtgcggatt gggggtgtgg
    59701 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
    59761 ttggctttct agggtcctac catttgtctt actgaaaaaa ttcgatttac cgcaaccgtt
    59821 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
    59881 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
    59941 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggcaggaat
    60001 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
    60061 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
    60121 tatccgttga acggaccact aacaacgact tttcctaaca tatgtcctgt ttctacgtct
    60181 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
    60241 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
    60301 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
    60361 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
    60421 ccaaatggtc ggacaaggtg cttgatttga tcaaagtagc gggtaatatc gaataataca
    60481 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
    60541 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
    60601 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
    60661 ccggatccgt tgatcactaa gatttttttc ccgcgattgg cattttcttc tggagttaga
    60721 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcagct
    60781 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac tagttggtat
    60841 tcttgattgc ttcctgctct tgttgttgta cctgcgtaaa aaacgcgatc gccgacagaa
    60901 aatttttgca cttcgtcacc tacagcaaca actttcccga ctccatcaaa acctaagata
    60961 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
    61021 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
    61081 tcaataaagc tttttggatc atcgatcggc aacccttcat aaaaaccgac taccttcatt
    61141 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
    61201 tgttcatttc aatatatata gtatgctatt tgttccacct tttttattca tcataattat
    61261 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
    61321 ttttgtcaca acctcttata aacggtgttc tcctaccaac tcttcggtca caagtcaaaa
    61381 tattcatcaa acatgagaag ctgttttttg aaattgctcc ttgtgactca gagttaaacg
    61441 gcaggctcac aacctctgat ataaacggtg ttcaatcaac tgaccctcgg tattagctca
    61501 aaaaacgaca aaatatgaag agctattttt gtttggctgt tcttattact tgatacagga
    61561 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
    61621 gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca gtagatttcg
    61681 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
    61741 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
    61801 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc gggtcttttt
    61861 caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca acgccatatt
    61921 tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc gtgattagtg
    61981 tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc caatgtgcat
    62041 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
    62101 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
    62161 tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc gctacttctg
    62221 gacgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
    62281 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
    62341 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaatgtta
    62401 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
    62461 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
    62521 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
    62581 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
    62641 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
    62701 ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt tccggaataa
    62761 atggtgttca aaagcttgca tttgcggtaa taagtccaac caaacgaaaa ataggaggag
    62821 ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac aacctctcca
    62881 ctaaacggtg ttcaatcagc tgaccctcgg tattcgctca aaaaacaaca aaatatgagg
    62941 aactattttc ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    63001 ttctaacttc tcaatcctct tcctttattg atcaaatacc aagtaacggc gtacaaaaca
    63061 acgatgaata acacaagtac gatcatcgat gttgctactg gcacatcgat cgttccaagg
    63121 aatccgtaac ggaaaccgga gatcatataa acgatcggat tgacttttga aacagcttgc
    63181 caaaagggcg gtaacataga gatggcatag aaaacgccac ctagataagt taacggctgc
    63241 aagacaaatg taggcacgat cgatacatca tcataagact gagcaaaaat cccgttgagc
    63301 agtcctgcta atgaaaacaa gattgccgtc atcaataatg taatgatgac catgaaccat
    63361 gaatacacat gaagtggtac aaagaacaag gaaatgatcg ttacgagaga gccgacgaga
    63421 atactgcgac ccacaccacc aatcacaaag ccccaaataa tcacatgcgt aggaacaggt
    63481 gctaccaaga tttcttcgat atttttctga aatttttgag agaagaagga cgaagaaaca
    63541 ttggcatacg aactagtgat agcagacatc ataatcaaac caggaacgat gaattccata
    63601 tatgaaaaac cacccatgtc accgatccgc ccaccgatca tttttccgaa aataacaaaa
    63661 tacaaggatg tcgtgatcac tggcggaacc agtgtttgga cccatatacg caaatagcgg
    63721 ttcgtttcct tgatagctaa actttttaat gcagtaaaat aaagattaaa catttttctc
    63781 ctccgtatga ttattttctt ctgtgatttt caagaacagt tcttctaaac gattcgactt
    63841 attcctcata gaaagaactt tgattccttg tcttgtaagc tgatcgaaga ttccgttcac
    63901 cccttgattt cgttcaactt cgacagcgat tgtctgctca tcttcaaatg tacaagcata
    63961 tccttcaatc acaggtttgg tatcatatgg cgctaaatca aagataaatg tttcaaattg
    64021 cagttttgac agaagctctt tcatacttgt attctcgatc agttcacccg attggatgat
    64081 tccgatgttg cgacaaagca tttcagcttc ttctagataa tgcgtcgtca aaatgatcgt
    64141 tgtaccattt tcgttcaatt ctcgtagaaa ctcccacatc tcgcgtctca actcgatgtc
    64201 aactcctgct gttggttcat ccaagatcag caaacgtggt tcatgcatca atgccctagc
    64261 aatcatcagt cttcgcttca ttcccccgga aagcatacgt gcacggacat ttctcttttc
    64321 ccaaagattg gactgcttca aatatttttc gctgcgtttc atcgcttctt tgcgagagac
    64381 accgtagtag cctgcttgat tcacgacgat ttgctggacc gtttcaaaag ggttaaaatt
    64441 gaactcttgt ggaaccaatc caatctgttg ctttgctcga actagatcct tgtccagatc
    64501 ataaccgaag acattcactt ttccagatgt tttattgact aaagaagtaa taatcccgat
    64561 cgttgtagac ttcccagctc catttggccc taatagcgca tagaaatctc cttctttcac
    64621 cgaaagatcg attccccgca atgcttcgac cccacttcca tagatctttt tcaaattatt
    64681 tatttcaagc gcatttgcca tttatttcct atctccttat agacatgatt cttaattgta
    64741 tcatggaata cagcagatga aaaaacaact tggcttccac acaaaaacac acctgctaac
    64801 aaagaggaga tgtgttttct agttcatgat catcttcgat agaataacta tctaaacaac
    64861 tctggaactt ttaaaatcat aaaagtaccg acagcaacta ctacgatgac atatagaaga
    64921 tccttataac tgaggttctt gtctttttta ttcatgttaa gctttcctcc gtccttttat
    64981 cgaaagcttt tttcttttat tttaaccgaa ataaataaaa tggaaatagt tattttcaat
    65041 ttctgttttc gctgtttgtt ttacggagaa ctttgccatg gaacgtgcgt ctcgtcgctg
    65101 atgctaagtt ttttcgataa aagctgctag aaggaagaca gcgtggttta ttttgtatcg
    65161 tcttttgcta aatcttccaa gatcatctgg acagcagact cgttcccatt gaattctgcc
    65221 acttttttgg catcaatgta cttcttttga taatcgtctt tttggaccca cgcatcatgc
    65281 ttcgtttgat aaagttaatc aaacggatac cactgacgct gttcatcgaa caacaaactt
    65341 tctttttggg cctgttcaac tatggattta ggataattca aaactttcag caaggcaatc
    65401 gcatttcggg tccttgaagg tccttctttt aatttgaaat caaaactgat tccttttcct
    65461 tctgttactt gttcttcaaa gtggacattc tcacaataat tctttaaaat ctctgtcaat
    65521 tcgatatcat gtgtggcgac aaatgctaaa gatggatact ccgcaagcca ccgaacgact
    65581 gaagcggaag aagcaatccg ttcgatcgta tttgttcctt ttaaaatctc atctacgaag
    65641 cagtagcagc gttctttgga agccacttgg tccagcaaac gtttgatcga tttgatttct
    65701 gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga ttgccatgga agtcaacaca
    65761 tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt ggatcgtctg tgctagaata
    65821 cagctgatgg ctatactttt gacatacgta gattttcctg atgcattgga acccgtgact
    65881 aatgtattct tctgccagtt gactggatta actaccgctt cttttaacaa tggatgatag
    65941 atatctttcg ctgtcacacc gccctcagta aactctggtc ggcaagtgat cggcatatag
    66001 gtgcgataat tcaaaacggc tgctgcaact tccaattctc ccagcaattc ccacagcttg
    66061 atggcttcag ttgaatgctt ttctaattta gtgagaacga aatgatagga gataaatggc
    66121 agcatgaaca tgatgttgac atattcaaaa atcaattctg cttcactccc gctcttcatt
    66181 cgaaatgaaa aacctaattt agggatgtct cttaatggtt tagtttgctg tttcagctct
    66241 ttttgaagtg gtgtcgtcat ttttgctaac tgattggcgg ctgaaatcgt tgaaaccaaa
    66301 taactcatgc tattcagctc tgtttctagt gtttgtttct tcactagata atagaccgta
    66361 ttgaatacta agccaccgat tgctagatag attcctagta cttgccctaa aagaagcaaa
    66421 cctacaccca ccagcggaag caagccaaga aaaataaata aacctacgcg tccaatcttt
    66481 ttactttttc catccgctag atattgtttt gcaaaattat gatctttttt tcctaaacga
    66541 gcgaattgat attggattct ttctctcagt tgaggatttt cctgataaaa agcaattagt
    66601 ttttctaact gataatcttc tccaaaatca aagctacgta aacgctgata caacgcttct
    66661 gaacctacac tagaataggt actgttgatc ccttcaaata cttcgaacat atccaagtca
    66721 taccaagtaa gatcgtcgat ttcactatcc catgaacgga attttttctc cgtgagccac
    66781 gcatccttca aactttcttc tttatcaaat ctcggttgat aaggaatttt tccccaatta
    66841 ctacgaactg tttcctttac tttcaaacgt gaaaaaatgt ctaaaacaac taataaaata
    66901 acaactgcaa taataattaa aacgataaat tctctttggc ccatatccct ctccgttcct
    66961 tgatttgtta gttttagtat agcactgttg cgctatttga aaagggggta caaaaataac
    67021 tgtcttcgtt acctaattcg tatcaatcac accaaaataa aaacaccttc caaaaaagaa
    67081 gatgtcttta aaacgagata caattttttg tcattcactt caggtaaatt agagccaatt
    67141 aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct
    67201 ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg
    67261 tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt
    67321 gcgctcctgg tttgatagga ttggccactg ttaatcgaat gaatgaatcc tcttttgaaa
    67381 atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca
    67441 ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg
    67501 ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct
    67561 ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa
    67621 gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta
    67681 aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa
    67741 tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt
    67801 cttccatttc ttgaaatgct tgtccttttt gttcattttc atccacccat gtattcagtg
    67861 catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt
    67921 cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga
    67981 agaaaataaa acttcccata aagataacaa cgagaagcaa cagccactta atcaaatcta
    68041 aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag tttccctcga
    68101 agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact
    68161 gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc
    68221 agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac
    68281 tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca
    68341 gaagtctgat gacgaagttg gatgccgatt cgtaacaaca gttcttcgat atcaaatggt
    68401 ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt
    68461 tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct
    68521 tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt
    68581 aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga
    68641 acctcctcca gtaaagaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc
    68701 aggaacacct acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc
    68761 acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc
    68821 gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct
    68881 ttgatttttg cgaaaacacc gggaacgaag gccgttccca tgatataaag aaaagaaaga
    68941 agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc
    69001 gctaatatca gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg
    69061 attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caatcatggc tagccccgta
    69121 atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa
    69181 gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc
    69241 ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttcc
    69301 accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg
    69361 ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc
    69421 agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc agcagttaat
    69481 agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc
    69541 aactaggtgg ataccatcca caggaacttt tgcctctacc aatgcttgat tgatggctgc
    69601 tacttggtca attccttcaa aaagctggat aacacggcta gattggacaa aataatccct
    69661 ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat
    69721 aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc
    69781 atgcaaaata ccgtagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag
    69841 caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat
    69901 ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat
    69961 cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt
    70021 tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac
    70081 accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc
    70141 aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa
    70201 taattcgatc gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga
    70261 cttacccgct ccattctccc caatcaaacc atagatcatt ccacgtttga tttcgatcga
    70321 tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac
    70381 cgtttcattt ccaaaatcca tacttatcct tctttcttat tgataagtga agtatagttg
    70441 acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatt
    70501 tcaagaaaaa tcagactgtc tcgcagacgt ggttgagttg tctgccaatc aaagagcgca
    70561 ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacatc
    70621 agacgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa
    70681 gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac
    70741 aattagctac caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata
    70801 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag
    70861 caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa
    70921 gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa
    70981 tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt
    71041 tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt
    71101 cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag
    71161 aactgtgaag ctttacgggc tttcttaaga cctggttaaa caaaactcta ttttattgca
    71221 ttttacagga atgtatttca tgcgaaatta ataagtcttt gtttatagag ttttactagg
    71281 gtataaaaag ttgtatgtta tcattacctc aaaacattac ctcagcttgg cgagttggtt
    71341 gattgaagaa taaatcattt atctagatat ctcaataaaa aatgatttct aagaaacttg
    71401 tattttcgtg ttattatttt tttgccctat caaaatttct aattcttcta gcgtaatatg
    71461 attatgacac ataatcgatt caattacttc aatacctatt tttttcgaga aagatccctt
    71521 ttgttcactc acaaaaatac tttcagaaga catatataag gaacgaagtt ttggaattag
    71581 tatatcgtac aaatagacca ccataaaatc gtccttgcaa ccattctcaa aaagaattaa
    71641 atttgctgat tgtgttacat atatcactga attacattca tggtcaattc caaaaacaat
    71701 gtctacttca ttttcttttc taattcgcgc agttacattt ttcaaacttt caccattact
    71761 tataaatgct ttagttactg taagctctga tgtttgttca gatattcctt ttataatatt
    71821 ttgttcatca gccacattcc ccataaatcc gccgattcct tttaagtaat aattctcacc
    71881 taagagtcgc ttgcattcac tgaattgata aaaaaaccag ttaaaaaaat cttctggaaa
    71941 aataaattct gatcgaactt tatttatatt tttagctcct attaatttta taactcgatt
    72001 aatgttcact tgctgcgatg aacatataac tgaataaatt gagttgttta attcaaaaaa
    72061 acaactttaa catatgcatc attaattcgt tattttttag gtagatattg atcattgtta
    72121 tctctctttt tttgcttccc atactccaac ttagaaattg ccgttataaa atttatttta
    72181 aaggtgactt tttcccctga agtatcggta gtaggaatac ttaatacctc agcttctgag
    72241 gaggtatccc aattatagtt atactttgca tcatcaccat aaagaacaat tccatttttt
    72301 ttatcttgtg ccgttagctt tactggttcg cttacttttc ttgttaatat cttggacttt
    72361 acatcatcga tattgcttat tttatcaagc ttaaataatt tacaaattaa tttcgtacta
    72421 gtttttatat ttgacgagag tgtaaaatat tttgtgtaaa tagaaaaaag gaagtccctt
    72481 ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata tgaacgattt
    72541 tactacagaa attctaaaga ctctagcgaa caaaggcgat ttgaatgaat tattccgtgt
    72601 ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt tcctcgatta
    72661 cgaaaagtac gatcgcattg gttttaacac ggggaattct cgtaacggct cctatgaccg
    72721 tacggtcaag accgagtacg gggaacttca tctccagatt ccgcgcgacc gcaacggcga
    72781 gttcaagcaa cagactgttc ctgcttatag acggacgaat gatatcttag aggagaccgt
    72841 cattcacctc ttccgaaaag gtattaccat gtcggaaatc gcagacttga ttgagaaaat
    72901 gtatgggcat cactacacgc cccaaaccat gtccaacatg actaaagtgc tgactgaaga
    72961 agtaaatgca tttaaagtta gaactctaaa tgataagtat gtcgctattt tcatggatgc
    73021 tacttatatt cctttaaaac ggcaaaccgt ctccaaagaa gcaatctata ttgccattgg
    73081 tatacgagaa gacggcacta aagaagtact aagttatgcg attgctccga ctgaatcgac
    73141 ctacgtttgg aatgagttgt tacaggacat ttactccaga ggggttcatg atgtcttact
    73201 gtttattacc gatggcttaa aaggcatgaa agatacgatt caccagattt atcctaaagc
    73261 taaataccaa cattgctgtg ttcacgtttc ccgtaatatt gctcataaag tacgtgtcaa
    73321 agatcgaaaa gaaatctgtg atgattttaa ggctgtttat caagctagct caaaggaaga
    73381 ggcaaatacc tttttaggga gtatgattga gaagtagcaa aaaacttatc ctaaagtgac
    73441 gcagtcactg ataaaaaatc aagatttatt gactttttat gagtttccgc ctggtatccg
    73501 ccgaagcatt tactcaacta atttaatcga atctttcaag aaacaaatca agaaatacag
    73561 ccgcagaaaa gagcagtttc aaaatgaaga atcactagag cgtttcctag tatccatctt
    73621 tgatacatac aatcaaaaat tcttaaatag aagccataaa ggcttccaac aagtgacgga
    73681 tacattagct tcaatgttta ctgagtaacc cattattttg cagaaggacg agttatttac
    73741 acaaaattat tgacgctccc tccaaattac ttcatcaaaa cattgatata atttttatta
    73801 tatcagtatt tattcttgat acataaatga gaaaataaat actttaaatc tattgactac
    73861 aagtagtcat taagtcttca taagatgtac aatatgacat taaaaaaata aaagcttacg
    73921 accatccgtt aaatctgaca ctaaataatg gactgctttc ttttgtatta gcaggttacg
    73981 ttgactatga atattcacca gattatttgg agaaacctct tcaacacgag aacaatgata
    74041 ttaattttag atttgtctac gagtcaaaaa gtaaaacatg gttagttgat caattaagca
    74101 cgataaacac aagcttccat gaattaccta aacctgaaaa tgaagttgag atggtcatta
    74161 aaatcttcta aaaaagaagc gttgaagggg aaatattcct ttcaacgctc ttttttatat
    74221 gcttagaaac ataaataaat tgtatacttt ctttttagcc cctattactt ttcacgaaaa
    74281 atatcttttt ttcgacgcta gcgtttctct acttctaata ctgtacacag tttttgtatt
    74341 attgaaaaat tactcattat ttagcattaa tccacatcgt tttatcaatc gaccttcata
    74401 ctaaactact ttaaagtcat cttccttctc gtttttcatt
//
LOCUS       DAPBBG010000008        70322 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPBBG010000008 DAPBBG010000000
VERSION     DAPBBG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 70322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.008"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(135..512)
                     /locus_tag="RD477_000606"
     CDS             complement(135..512)
                     /locus_tag="RD477_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7632992.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="RD477_000607"
     CDS             768..1496
                     /locus_tag="RD477_000607"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDT7632993.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="RD477_000608"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="RD477_000608"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDT7632994.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="RD477_000609"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="RD477_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDT7632995.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="RD477_000610"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="RD477_000610"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDT7632996.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="RD477_000611"
     CDS             4631..6070
                     /locus_tag="RD477_000611"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDT7632997.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6093..7145
                     /locus_tag="RD477_000612"
     CDS             6093..7145
                     /locus_tag="RD477_000612"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDT7632998.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="RD477_000613"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="RD477_000613"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDT7632999.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="RD477_000614"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="RD477_000614"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDT7633000.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="RD477_000615"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="RD477_000615"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDT7633001.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="RD477_000616"
     CDS             10664..11578
                     /locus_tag="RD477_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7633002.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="RD477_000617"
     CDS             complement(11711..13276)
                     /locus_tag="RD477_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7633003.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="RD477_000618"
     CDS             complement(13269..14720)
                     /locus_tag="RD477_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7633004.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="RD477_000619"
     CDS             complement(14734..15750)
                     /locus_tag="RD477_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7633005.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="RD477_000620"
     CDS             15970..17058
                     /locus_tag="RD477_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDT7633006.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="RD477_000621"
     CDS             17112..18638
                     /locus_tag="RD477_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDT7633007.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="RD477_000622"
     CDS             18640..19845
                     /locus_tag="RD477_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633008.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="RD477_000623"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="RD477_000623"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDT7633009.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="RD477_000624"
     CDS             21343..22767
                     /locus_tag="RD477_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDT7633010.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="RD477_000625"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="RD477_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDT7633011.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="RD477_000626"
     CDS             24340..25317
                     /locus_tag="RD477_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDT7633012.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25342..26985
                     /locus_tag="RD477_000627"
     CDS             25342..26985
                     /locus_tag="RD477_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDT7633013.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26993..28399
                     /gene="lpdA"
                     /locus_tag="RD477_000628"
     CDS             26993..28399
                     /gene="lpdA"
                     /locus_tag="RD477_000628"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDT7633014.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28561..28836
                     /locus_tag="RD477_000629"
     CDS             28561..28836
                     /locus_tag="RD477_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDT7633015.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28842..29609
                     /locus_tag="RD477_000630"
     CDS             28842..29609
                     /locus_tag="RD477_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDT7633016.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29777..31606
                     /gene="typA"
                     /locus_tag="RD477_000631"
     CDS             29777..31606
                     /gene="typA"
                     /locus_tag="RD477_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDT7633017.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31867..33639
                     /gene="recQ"
                     /locus_tag="RD477_000632"
     CDS             31867..33639
                     /gene="recQ"
                     /locus_tag="RD477_000632"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDT7633018.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33753..34055
                     /locus_tag="RD477_000633"
     CDS             33753..34055
                     /locus_tag="RD477_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDT7633019.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34058..35221
                     /locus_tag="RD477_000634"
     CDS             34058..35221
                     /locus_tag="RD477_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7633020.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35247..38675
                     /locus_tag="RD477_000635"
     CDS             35247..38675
                     /locus_tag="RD477_000635"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDT7633021.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38696..39820
                     /locus_tag="RD477_000636"
     CDS             38696..39820
                     /locus_tag="RD477_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633022.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39810..40247
                     /locus_tag="RD477_000637"
     CDS             39810..40247
                     /locus_tag="RD477_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7633023.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40231..40569
                     /locus_tag="RD477_000638"
     CDS             40231..40569
                     /locus_tag="RD477_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDT7633024.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40688..41248
                     /gene="rsmD"
                     /locus_tag="RD477_000639"
     CDS             40688..41248
                     /gene="rsmD"
                     /locus_tag="RD477_000639"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDT7633025.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41245..41736
                     /gene="coaD"
                     /locus_tag="RD477_000640"
     CDS             41245..41736
                     /gene="coaD"
                     /locus_tag="RD477_000640"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDT7633026.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41726..42769
                     /locus_tag="RD477_000641"
     CDS             41726..42769
                     /locus_tag="RD477_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDT7633027.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42928..43656
                     /locus_tag="RD477_000642"
     CDS             42928..43656
                     /locus_tag="RD477_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDT7633028.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43772..44017
                     /locus_tag="RD477_000643"
     CDS             43772..44017
                     /locus_tag="RD477_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7633029.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44014..44232
                     /locus_tag="RD477_000644"
     CDS             44014..44232
                     /locus_tag="RD477_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDT7633030.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44368..44907
                     /locus_tag="RD477_000645"
     CDS             44368..44907
                     /locus_tag="RD477_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDT7633031.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44900..47173
                     /locus_tag="RD477_000646"
     CDS             44900..47173
                     /locus_tag="RD477_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDT7633032.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47211..48257
                     /gene="holA"
                     /locus_tag="RD477_000647"
     CDS             47211..48257
                     /gene="holA"
                     /locus_tag="RD477_000647"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDT7633033.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48346..48600)
                     /locus_tag="RD477_000648"
     CDS             complement(48346..48600)
                     /locus_tag="RD477_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDT7633034.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(49000..49269)
                     /locus_tag="RD477_000649"
     CDS             complement(49000..49269)
                     /locus_tag="RD477_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633035.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49739..50194)
                     /locus_tag="RD477_000650"
     CDS             complement(49739..50194)
                     /locus_tag="RD477_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDT7633036.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50332..52260)
                     /locus_tag="RD477_000651"
     CDS             complement(50332..52260)
                     /locus_tag="RD477_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDT7633037.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52457..53290)
                     /locus_tag="RD477_000652"
     CDS             complement(52457..53290)
                     /locus_tag="RD477_000652"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDT7633038.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53482..54186)
                     /locus_tag="RD477_000653"
     CDS             complement(53482..54186)
                     /locus_tag="RD477_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7633039.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54209..54835)
                     /locus_tag="RD477_000654"
     CDS             complement(54209..54835)
                     /locus_tag="RD477_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7633040.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55161..57536
                     /locus_tag="RD477_000655"
     CDS             55161..57536
                     /locus_tag="RD477_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDT7633041.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57560..58051)
                     /locus_tag="RD477_000656"
     CDS             complement(57560..58051)
                     /locus_tag="RD477_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDT7633042.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58238..59158
                     /locus_tag="RD477_000657"
     CDS             58238..59158
                     /locus_tag="RD477_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7633043.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59312..59656
                     /locus_tag="RD477_000658"
     CDS             59312..59656
                     /locus_tag="RD477_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDT7633044.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59846..60346)
                     /locus_tag="RD477_000659"
     CDS             complement(59846..60346)
                     /locus_tag="RD477_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7633045.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60874..60957
                     /locus_tag="RD477_000660"
     tRNA            60874..60957
                     /locus_tag="RD477_000660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60908..60910,aa:Leu,seq:cag)
     gene            complement(61281..61919)
                     /locus_tag="RD477_000661"
     CDS             complement(61281..61919)
                     /locus_tag="RD477_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7633046.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            62254..62688
                     /locus_tag="RD477_000662"
     CDS             62254..62688
                     /locus_tag="RD477_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7633047.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            62711..63031
                     /locus_tag="RD477_000663"
     CDS             62711..63031
                     /locus_tag="RD477_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633048.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            63172..63363
                     /locus_tag="RD477_000664"
     CDS             63172..63363
                     /locus_tag="RD477_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633049.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(63434..63691)
                     /locus_tag="RD477_000665"
     CDS             complement(63434..63691)
                     /locus_tag="RD477_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDT7633050.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            63849..64112
                     /locus_tag="RD477_000666"
     CDS             63849..64112
                     /locus_tag="RD477_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633051.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            64136..64324
                     /locus_tag="RD477_000667"
     CDS             64136..64324
                     /locus_tag="RD477_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633052.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(64407..64592)
                     /locus_tag="RD477_000668"
     CDS             complement(64407..64592)
                     /locus_tag="RD477_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633053.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            64807..65178
                     /locus_tag="RD477_000669"
     CDS             64807..65178
                     /locus_tag="RD477_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7633054.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(65328..65400)
                     /locus_tag="RD477_000670"
     tRNA            complement(65328..65400)
                     /locus_tag="RD477_000670"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65365..65367),aa:Gln,seq:ctg)
     gene            complement(65446..65874)
                     /locus_tag="RD477_000671"
     CDS             complement(65446..65874)
                     /locus_tag="RD477_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633055.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(65934..66692)
                     /locus_tag="RD477_000672"
     CDS             complement(65934..66692)
                     /locus_tag="RD477_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7633056.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            66987..67184
                     /locus_tag="RD477_000673"
     CDS             66987..67184
                     /locus_tag="RD477_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633057.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            67290..67772
                     /locus_tag="RD477_000674"
     CDS             67290..67772
                     /locus_tag="RD477_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633058.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(67830..68426)
                     /locus_tag="RD477_000675"
     CDS             complement(67830..68426)
                     /locus_tag="RD477_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7633059.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            68813..70279
                     /locus_tag="RD477_000676"
     CDS             68813..70279
                     /locus_tag="RD477_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDT7633060.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        23065 a        12027 c        14915 g        20315 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
    25381 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    25441 gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca
    25501 ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta
    25561 gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa
    25621 tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg
    25681 tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt
    25741 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    25801 gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa
    25861 aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat
    25921 gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa
    25981 aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta
    26041 ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta
    26101 actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt
    26161 ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca
    26221 gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga
    26281 gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc
    26341 aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta
    26401 tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc
    26461 ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca
    26521 tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca
    26581 actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg
    26641 tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct
    26701 gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt
    26761 tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca
    26821 caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca
    26881 ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc
    26941 aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt
    27001 tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt
    27061 agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat
    27121 tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca
    27181 ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact
    27241 agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg
    27301 tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt
    27361 agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa
    27421 cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg
    27481 tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat
    27541 cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt
    27601 aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt
    27661 agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga
    27721 agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc
    27781 cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg
    27841 cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg
    27901 acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc
    27961 acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc
    28021 tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg
    28081 tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt
    28141 ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac
    28201 gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat
    28261 ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac
    28321 gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg
    28381 tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc
    28441 gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc
    28501 ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag
    28561 atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta
    28621 atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt
    28681 ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg
    28741 ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa
    28801 aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc
    28861 atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa
    28921 caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga
    28981 attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag
    29041 aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg
    29101 gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat
    29161 ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg
    29221 gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct
    29281 tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc
    29341 gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag
    29401 tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg
    29461 cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc
    29521 ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa
    29581 tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat
    29641 aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga
    29701 aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa
    29761 aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca
    29821 cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga
    29881 cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg
    29941 tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat
    30001 ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga
    30061 cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt
    30121 gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc
    30181 ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc
    30241 agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag
    30301 cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat
    30361 tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt
    30421 acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat
    30481 caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt
    30541 aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg
    30601 agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt
    30661 ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt
    30721 cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat
    30781 tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa
    30841 ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga
    30901 aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga
    30961 aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata
    31021 tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca
    31081 tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta
    31141 ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca
    31201 atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat
    31261 cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt
    31321 cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa
    31381 tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa
    31441 agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt
    31501 cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat
    31561 tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg
    31621 aagagagaaa ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc
    31681 tgtggagaag tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta
    31741 aaagactttt aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca
    31801 cgaaaggaga tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc
    31861 gggaagatga acgacttatt aaaacaatac tttggctatg atgagtttag acctggtcaa
    31921 aaagaaatta tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga
    31981 agtgggaaat caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt
    32041 gtgtcccctt taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata
    32101 cccgccactt ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa
    32161 atcgatagac agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat
    32221 tttatccaag caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt
    32281 atctctcagt gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat
    32341 caactgccaa atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct
    32401 gctgacataa aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa
    32461 cgagaaaatc tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa
    32521 tatttaaaga tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta
    32581 gaccgtctct atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt
    32641 ctaaatgaga atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg
    32701 attgtagcga caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc
    32761 cattatcaaa ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga
    32821 gatggtcttt ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag
    32881 ttttttatcc agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta
    32941 aaagcgatga cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat
    33001 tttggcgaga caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata
    33061 gtagaagtaa cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac
    33121 tatggtaaac aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct
    33181 ttaggttttg gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg
    33241 atgcagttaa ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat
    33301 cctgttttga aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat
    33361 cgaaaggaac ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa
    33421 gtgctgcggg aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta
    33481 ttttcagatt ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg
    33541 cttcagataa aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct
    33601 cggataaaaa atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat
    33661 gaataaaaac aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat
    33721 aattaaggaa ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga
    33781 tccaagtatt aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca
    33841 gtctctgtat ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct
    33901 tttcccggca agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc
    33961 tgatgttaag tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa
    34021 tttatgataa aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag
    34081 gcatctatta gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat
    34141 catggtatat agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc
    34201 agtcattaac cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa
    34261 aatgaaaaca gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac
    34321 gattttgctg ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct
    34381 gcaaattgca ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta
    34441 cctgtctctg actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt
    34501 gaaacgtcca cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt
    34561 tggtaatgcg gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa
    34621 ttatgtgtat actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg
    34681 gctgatcaat atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg
    34741 atttgctatt taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa
    34801 cggctactat gccatgttca acggaggact atttggcagg ggattaggta acagtatcca
    34861 gaaaaaagga tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga
    34921 actgggagtg attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat
    34981 tgtccttgta ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg
    35041 cgcaatgttt ttgatccaag tatttgtcaa tcttggagga attactggcg ttattccttt
    35101 gacagggatc acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat
    35161 ttgtgtcggc tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata
    35221 ataaaaaaag atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa
    35281 tcgctgtacg tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg
    35341 cagaagaaga tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac
    35401 aaggaaaaaa accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag
    35461 aatgcggagc tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg
    35521 cgcaacggtg cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata
    35581 tttttggaga taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc
    35641 caggcacaga tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg
    35701 gttatccgat tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc
    35761 acgatgaaaa gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct
    35821 ttggtagtga tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac
    35881 agattctcgg ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc
    35941 agcgccgtca ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac
    36001 gtcaaaaaat ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag
    36061 gtacagtaga atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg
    36121 tccaagtgga acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac
    36181 tgttgattgc tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa
    36241 ttaaattaaa tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact
    36301 ttttaccaga cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc
    36361 tggatgtcgg aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag
    36421 taaaagtatg tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct
    36481 tgaaagaatt tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca
    36541 cacatcctga attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat
    36601 tatttgaatt tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa
    36661 ttactgtgaa tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac
    36721 gtatgcctaa agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg
    36781 cgaagggtgc cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg
    36841 atacgacatt tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt
    36901 tcaagcagat tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt
    36961 gggggggagc aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt
    37021 tgagaaaaat tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa
    37081 atgccgttgg gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag
    37141 cagcgcaagg aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg
    37201 aaaaaagtat ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata
    37261 ctggcgatat caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg
    37321 caaaggaact agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc
    37381 ttaaaccgca ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa
    37441 tccatctgca tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa
    37501 aagcaggagt agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc
    37561 ctagcatgag cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga
    37621 ttgaaaatgc acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt
    37681 ttgaaaacgg cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac
    37741 agtattcgaa tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa
    37801 tcaaacaaat gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat
    37861 catctaaagt agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag
    37921 atatttatga aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag
    37981 gagaactagg gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag
    38041 gcagacctgc attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag
    38101 taaaaaaaga attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct
    38161 atttgatgta tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg
    38221 ttacattact ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg
    38281 tacaaatcga acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata
    38341 tcgaagggaa tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca
    38401 aagataattc gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag
    38461 aacaaattgg agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata
    38521 acgttaaaaa aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg
    38581 aagcacgttt tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgt
    38641 ctggtgacct tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa
    38701 aagactgggt ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc
    38761 agtcttgttt ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca
    38821 aatgacaacg tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa
    38881 accgatcgat cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt
    38941 tggttttgaa acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat
    39001 ccaagacagc agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc
    39061 gttttattta gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt
    39121 cacttttgag taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta
    39181 tcgtccgcta attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac
    39241 aaacggccta ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata
    39301 tcaagtgttt ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc
    39361 aatcaatcgc gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga
    39421 cgaacttccc atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca
    39481 tgattttttg acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa
    39541 atcgctaggc agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa
    39601 gttaatcagt aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga
    39661 cgctactttt tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga
    39721 cccgggaacg gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca
    39781 agaagaaaac ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg
    39841 aggatcaatg cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc
    39901 ttgaacataa acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct
    39961 tgtctaagaa agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc
    40021 gtgaaaaaaa acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg
    40081 cagctttccg atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac
    40141 ttgccgaaga agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga
    40201 aaaaacactt tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata
    40261 acaaaacgac gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa
    40321 aagttcggct tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat
    40381 gaagagacat tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta
    40441 gaacgttctt accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca
    40501 gtattaaaag acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa
    40561 catctttaaa aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga
    40621 tttttgatca ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag
    40681 tgagtagatg cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg
    40741 ggcgaacacc agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc
    40801 atattttgat ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga
    40861 agcagtatct agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa
    40921 aatcatcaaa gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact
    40981 tgatgcggac aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt
    41041 tcttgatccg ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact
    41101 gggacttttt tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc
    41161 agaaactgtg gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac
    41221 tatctataga aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct
    41281 tttacaaaag gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta
    41341 attggtgttt ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca
    41401 ttgatcacaa aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa
    41461 ttgacggtag aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt
    41521 atcaaagact ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa
    41581 ttggaaactg tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta
    41641 aaagaagtac tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa
    41701 gctttagctg gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat
    41761 tatcatattt ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga
    41821 atcaccggga gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa
    41881 aggttctggg tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc
    41941 tttaaaagct aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg
    42001 ggcgacaaat gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc
    42061 agcaatcgaa caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt
    42121 atatgtcatg agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac
    42181 agtaacaaaa gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa
    42241 aagccaaaaa gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac
    42301 agcttctggt gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct
    42361 tactgaccat accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg
    42421 tgggccatca gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa
    42481 cttgcgacat ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg
    42541 ccgaatcgga ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt
    42601 ctttgcacca gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac
    42661 caactacgaa gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc
    42721 agtaaaaaca gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat
    42781 taaagagggg ttgtgacacg agttctgtca caacccctct ttttcgtcaa aaatcttcac
    42841 gataaaaata aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg acgattttgc
    42901 cagagctcac tgtttcttca attttaaatg aaaggagtaa tgaatgattt ttactggtca
    42961 ttctttgaaa aaagaacaat gatagaaaaa atcagcaaga aaatactagc ttcacccaaa
    43021 ttgatcattt ggatgtctat gatagttctt atcttttgta tcgcaagtgg ttttttatta
    43081 tcaaatttgc tattttatag agaaacagaa gagactgctg caaaagaaat actttcttcg
    43141 tttagttcca gtggagaaga tccatcagct gaaggaaacg atgtagaaga ggatgctcgt
    43201 ccaaaggtca tgtacaccga tatcaaagga agtgtgaagg agcctggtat ctattccttt
    43261 tcttcagaag aacgggtcta tgatgtacta aagcgtgcag gtggattatt agaagaagcg
    43321 gatagcgatc gaatcaactt ttctgcgaag atagaagatc agcaagtcct atatatccct
    43381 gctgtaggtg aagagctgcc agaacatctt gatcagttgg catcctcgga aggaaaacag
    43441 cctacagcag attccgagcc tcccaaaata aatatcaata cagccactcc atcagaactt
    43501 cagcagattc ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa
    43561 aacggatcct tccagaaagt ggaagacctg caggagattt ctggtatcgg tgaaaagaca
    43621 gttgaaaaac taaaaaactt cgttacaatt aaatagaatg attcaaaatc gactggcacg
    43681 agcaataaat ggacgctaat ggatactaag aataatccaa ttctaagagc ttggcaggac
    43741 gaggaggtag tatttttgga taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa
    43801 accaatgctg aatgtctatc tccaaaataa aatatctggg attcatatta tgaatacggc
    43861 tgtcagcggc aataacagtc aagctctacg agaacgcttt gccaaagatg tacttagcta
    43921 tacagcagat aaagtattta ttctcattgg gacgaatgat ctagcagagc ataaacaact
    43981 ttctaaagaa acgtaccaaa aaatctgcag tggatgatag ttcagctgaa aaaagactat
    44041 agttctgatc atgctttctt tatcacgcca ccagcagtcg atgagacaaa acaaaaatat
    44101 cggaataaca agttgatcaa agaatatatc tctgttctca cagaagtagc aaaacgccat
    44161 gattgtgaag tatcgatttt tatacggaat ttcttgaaca aatggattcg aagaattttt
    44221 ataatgggct aatggatgac ggacttcatt tcggcagtga agggtaggat cttttgtcag
    44281 acaggatgat tcgttatatt taaagaatat tttgtttaag caatgagtac tttttaatca
    44341 acttgaaaaa acggaggaat ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg
    44401 aataaagaaa aagttactga taagtcacat aatgaacgca ttccttggga ccagtacttt
    44461 atggctcagg cagtactgtt atctttacgt agtacgtgta cacgtttaga agtaggagcg
    44521 acactcgttc gagaaaaaag gatcattgct ggcggctata atggagcagt cagtggagat
    44581 gtccactgca tagatgaagg gtgttacgtc gtggatggac actgcttgcg aacgatccat
    44641 gcagagatga atgcactcct acagtgtgca aaacttggta tcccgacgga aggttcagaa
    44701 atctatgtca cacattttcc atgtttggcc tgtacaaagg ctttgttgca agctggtgtg
    44761 aaagggatca attatctcca tgactatcga aatgatcctt atgcccaagc gttgatcgaa
    44821 caattaggtg tatctgtaca ccaagttgct ttagactcac agcatttttc aaaacttcaa
    44881 aatgaattaa caggaaaaca tgtctagcgt tttagaaagg catgttcgaa atcggttgat
    44941 ttttccggca attacgtcga tgattatctg tttgttgtat tacactgaca attggggtta
    45001 tcgtttgctt cttgccggaa tttttttatg gttttcgcta aaagaaggac gttttatcgc
    45061 gtacttgtgt attttgtgta gcataacggt atccatttct tataggctac aagagtattc
    45121 atttttagag gaaaaaacaa catctgcgca aattactgta tttacagata cgattcgtgc
    45181 aaatggtaat tttttgacaa tggaaggaag gttatcagag aataaacaaa agctccagct
    45241 ttcttatcaa gtaaacacag aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg
    45301 aatagaattg actgctgaag gcgagttcat ctctccagca aaaaagcgga atctttataa
    45361 ttttgaccaa gaagcttact tcaggaacca tagaatttct ggacggttcc aaattcaaaa
    45421 gattgaacag atcaccatgc aaaatcactg gcgaaactgg cttcagagaa aaagaggatc
    45481 ttttatctcc tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt
    45541 attatggggc tataaagatg caggattcag agaaccagaa gaaattttta gggagagtgg
    45601 tttgctgcat ctgttcagtc tatcgggtct acacatccaa ttttatttag ggtggctgta
    45661 ttatttattt cgacgtatcg gatggccact acacgtcagt attattccgt tgagtctact
    45721 aactatctgt tatgtttcta ttgcaggaag ttctatttct gttttgcgtg ctagtttgct
    45781 gtttttattg cgattgctag tcaaattgct gaataagcat tattccacca tggacttatt
    45841 tgcagttgtc ttttggcttc ttttactatt tgatcctcga tttctttttc atgcgggtgg
    45901 acagcttagt tttttcatga gttttttgtt tatcctgatt tcttttgatg gagattggtt
    45961 gaaaaaaacg gcctctcacg tcctgtttac tatgatcacg atgccgttga ttgtatggca
    46021 tttttatgag tggcctattt taggtagtct ttttacatta ctgattgcac cggtattcaa
    46081 gtttcttttt cttcctggtg tgttatttct cacactcttc aatcgctggc tgccggaaac
    46141 tttacttttg ctgatagaag agggattaat cttatttgag cgaataatcg atttttcttc
    46201 agggtcgtct ctcattatag gacgtttgcc ccttgtgcta tctgggatgt atattttcgg
    46261 attactgatt tgtatggata ggttgaaaga acaacctgtg aaacacttgt tttatgttgg
    46321 tcttttttct tttatttttt tgactttgcc atttttacgc ttttcttcaa cgatcgcttt
    46381 tatcgatgtc ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa aagaaacgat
    46441 tttgatcgat actggaggtc agttacactt tgaaagagaa aagtgggcgc aaggtatgat
    46501 acgtcctcct gctgaaagaa atatcgttcc atatctaaaa ggtcagggga taagtgtaat
    46561 cgataaactc atcttgacac acgacgatac ggatcatgtg ggagaattac ccacgttatc
    46621 tcagcatttt actatcaaaa aaatttatat cggttggggc gctggacgaa atgagccatt
    46681 acaaaggcat ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg
    46741 ggacaggatc agcggttatt atgatcttta tgtattgact ccttttgaaa agggagaggg
    46801 aagaaatgaa gattcgattg gtctatggat ggaatataac aataggcgct ttttattttt
    46861 aggagatttg aatcaagcaa tggaaaaaca attgcttttg atttatccta atctgaaagc
    46921 cgatgtagtc aaattaggtc atcacggtag ccgtacgtca agtaacacag attttctttc
    46981 ccatattgaa gcaaaacatg ggattatatc ctgcggcgta aataatcgtt atggtcaccc
    47041 gcacagcgaa gtattggaga cactagaaga aaatcagata aaaacatggc gaacggatca
    47101 acaaggaatg atcacctatc gctggcatcc agtttttcat cctcatgggc tagtggaaac
    47161 aatgatagac tagtaattct catctgctca tggtaggata atttttgaaa gtggtgagta
    47221 atttgagtat acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact
    47281 tgattttagg aacagaaaag tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa
    47341 tccaattaga gggcgaagat gacttaaatt ttttaagttt tgacatggaa aatgcaagtt
    47401 tagatgaggt agtagctgaa gcagagacgt tgccgttttt cggagatcaa cggttagtat
    47461 tcgtagagaa tccttatttt ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata
    47521 ctgatttatt gacggcttat ttgaaagaac cattagaatc gactgtatta gtattctttg
    47581 cgccatatga aaagttggat gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa
    47641 ttatcatcaa tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg atgaatacct
    47701 tagagaattc ctcaatcaaa atggaacgaa gtgcaattga tttattcttg aggttgaccg
    47761 acttagattt atccaaaatg atgagagagt tacagaaatt ggtcctttat ggacaaaatc
    47821 aacagcaaat cacggtaaga gaagtagaag aattagttcc taaaacgtta gaacataata
    47881 tttttgacat gacccaatat attttaaaag ggaagacgga acaagctttg cgtttatacg
    47941 aagatttagt tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat
    48001 tacgtttatt tttacagaca aaatttcttg taaagattgg ttatcagcag gcaaatattg
    48061 ctgaaacatt gaaaatccat ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat
    48121 tcgatgaaca tttgctggta cagttgtttg atcaattagt tgagatggat tatcaaatca
    48181 aaacgggaca aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc acagggcagt
    48241 tgttaagaaa tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt
    48301 tgtccttttt ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt
    48361 tttgcttaaa cgagatttgt cgcggttagc tttgtttttg tggattagac cttttgattc
    48421 agccatatca atagctttaa cagcttcttt atataacgca tctacgttgt cagcacctga
    48481 agctacagca tcttcaaatt ttttgattgc agtacgcata gcgcttgttt gagatgaatt
    48541 tttcacatta gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa tgtttggcat
    48601 tagtttcacc tccgataact taagttctta cgcgtgttac cgcataattc aacattcatt
    48661 attataccta aaggcagcgt gctttgcaat atgaacatga caagtttttt tccttaacag
    48721 tcttttatta ggattttttt tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa
    48781 gacgctattg tgggaggcaa tagcgtcaaa ccaaactaga tttttattta tctatttaaa
    48841 caaagtttga atgggaatgt ttggtactaa gttattatat aacaaagaga accgtttgca
    48901 aagttttttt tgaaaaggga tacgattccc tgttgttctt tgtttttcta ttaagaaact
    48961 gcagttattt tataaccagt atcctttgat cagtaaaggc tattctggtg taccgattat
    49021 tttaatgtat tcatcgatat tttttatcaa catttttttt atgaccaact ggttccgacg
    49081 gttccatttg ccacttactt ccagcacata tttatttagg ggcaaacgta agatatattt
    49141 tgccaaatag cgatctttaa gcaggccatt gatcacacgg tcctcaagct gcaagttgat
    49201 cccagtcagc atcattggct gttcttgtaa aacgtacgta tcaattaaaa gaccttcaaa
    49261 agtttccaca agcgtcttcc tttcttttca aagtaacctg aaaacaggca ctaattcttc
    49321 cattttgata ttcgttgaag tggatgcgat cattctttct tttgtccact gtaatctagt
    49381 ataacaaaaa agagcagaag agccaaaaag aaaaaagagc atcgaattct ataggaagtc
    49441 tcattggcac ggcctaaatt attacaaaac attgattaaa aaaagcccct gcatcgcagg
    49501 agcatcccct atatcgtttt aataggtcaa tcaaaaagca gtatcaaaca cattttgtat
    49561 caaatcagga aataatagat aagctaagcc cagaatcaaa agtaccgcta gaagaaacca
    49621 gccgattgtt cctatgaaat actttaattt ttcgaacatt gtatcacctc gttgtcagta
    49681 tatcacggat cagtaaattg ggggtgttaa gaatctccta aaatagacaa aatttgttct
    49741 attttcctaa agcaaatact tgtaagatac agtaagtgat accagaagca atgaccggtc
    49801 cggcggcgat tcctcttaag aagacaacac ctaaaatcgt gccgaaaaca agtgcaacag
    49861 tgatttgtgg atcagaagca atcaatccta cacccttgga agataagaca gccactagcc
    49921 caccgcaaaa aacagctacc cagccaatag gggatttaaa ggcttctaat agatcacgta
    49981 gaccgatttc tcctgttgcg atagggatta gaatagccgc tgtgatgatc gtcaccccaa
    50041 tattgattcc tttgccttga agccaaggga aaaaggaacg ggaggctggt atgagcttca
    50101 taatcaaaac aaaagctgcc gctatcatca gactttgatt tttagctaaa attgctacaa
    50161 gtaaaataat tcctaaaaaa agccaacttt ccatgaaata tattcctcac tttcttcgaa
    50221 acaatcataa catgttttta gaaagttaca aaagtaggaa ttcatccttc aagacctatt
    50281 cataagctgt ttttttcata aaataaacaa aatcataaaa aaacactttg cttatttgac
    50341 agaagacagc cgcgctttgt atagtggaca agacttacat ttttttgaag aatcagaaag
    50401 atcaggtaaa ggtcttagta aacgcaagcc attcagaata accaatattg tgcttccttc
    50461 gtgtcctaca actccaagag gtagatttaa aaattgcagg aagttagaag caatcaacaa
    50521 gagaataaca agaatcgaaa aaataatatt ttgtttgatg atccgccgca tttttaaaga
    50581 tagccgatga ctgtagacaa atttatttaa gtcgcttttc attaagacga tgtctgcgat
    50641 atccatagcg gcatctgtcc cttgccccat agcgacacct aaagaagcgg ctgctaaagc
    50701 aggcgcatca ttgactccat caccaaccat cgcatttact tggtaaagtt cattctcttt
    50761 tttgactagt tgtgtttttt cttccggagt acagcttgca tagtaatgtt caatccctac
    50821 ttgttcagca atggcctttg ctgtgccaga atggtcgccg gttatcatac ttgtatggat
    50881 gttttgggat ttaaagtagg aaattacttg ttgggtattt gcttttggaa tatctagcag
    50941 acctaaaaca gcaattaact gctgatcttt agaaagataa atcacggttt tgccttgatt
    51001 ttccaaacgt tcgatcattt ccgcaatctc tttgctgatt atcatcgtct caggatcata
    51061 tgcgtgcttg ccaactttcc attgcgcacc taaataggta gtttgtaatc caaaaccagt
    51121 aatttcttct attggtaagt attggatttc ttcaggtatt gtgcaatcga aatattgcaa
    51181 aatcgcatga gcaagtggat gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc
    51241 caaatccttt tcatcaaaga aaaagctgtc agttacaact ggttttcctt tagtgagcgt
    51301 tcctgtttta tcaaaggcaa tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc
    51361 tttgattaaa atcccatttt ttgcagaatt tgaaagagca gctagagaag ctggtgtagc
    51421 agatgcgaca agcgcacaag gagaagccac aactaataat accattcctc gataaaagct
    51481 ttcatcccaa ctccaaccta ggaaaaagtg ggggagaagg atcattagag gaacagcaag
    51541 caaaatcaat tttacataat tattttcgat tcgttcgata aatgaagctg tttgagttgg
    51601 cgtattctgc gcttcttcta ctaattggat aatcttagca aagacagttt cggaactcgt
    51661 ttttgttact cgaatcgtga tgggatttcc ttgattcagc gttcctgcat acatctcatc
    51721 gcctaaatat ttttctacag gcacagattc accgcttaat gcagactcat cgatcgttgc
    51781 ctgtgtgctt tctaaaaagc catctatcgg aacagctgct cctttagcga caaataccaa
    51841 gttatccacc tttagttgag aaacatctac ttcttgcatt tgtccatttt cttttaataa
    51901 ttgggctttt tgtggttgaa gattcatcaa agcagtaatt tctttttgac ttttattagt
    51961 tgtgtattcc tctaatgcac cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa
    52021 gtaattaccg atcaaacagg caccaatagc agctaaagcc atcaataaat ccacattcag
    52081 tgtccgatca ttccacaatt ctttcaagcc ttccgatgtt tgtttatatc caccgatgat
    52141 aatggcactg atataaaaaa gtggggtgat ggtaagagag aatcggtcga agataaaacc
    52201 gaagatcata aataaaaggc agccgattgt tgctgcaaaa gcttttttct gattagtcat
    52261 cgttattaga cctccttgtc aaaacaagga tagcataata aaaatgtaat tgataataat
    52321 aacagctaaa tgagaacgat aatggttatc atttggtgat taagcttgat gttgttttta
    52381 ttatttataa tgattattat taaagaaaat aaaaaaccag aacatttctc aaataagaga
    52441 agtgttctgg ttttttctac attgcaataa accaataacc aatcaaaatg ataccaagga
    52501 taatacgata ccagccaaaa gcggtaaagt cgtttttctt caaatagctt agtaagaatt
    52561 tgatagctaa gacggaaacc acaaaagata cgaatgttcc tacaagaaga acaacagttt
    52621 cttccatttt gaatgaattc ccttcagcta aatatttgac gatcttcaag ccgcttgcgc
    52681 caaacatgac aggaatccct aagaaaaatg aaaactctgt agcgacaaaa cgcgaagcac
    52741 caatcaagat agatcctagg atcgttgcgc cagaacggga tgttccggga acaagtgcca
    52801 agacttggaa taacccaaca agaagagctg cttggtacgt aaagtcattc agagaagtcc
    52861 aacgaggtgt tttgtttttg ttgcgttttt cgacaataat aaaggcaata ccgtaaacga
    52921 tcaaaacaat ggcaactggt aagaacttgt ggaaatttgc ttctaaccaa tcatccaaag
    52981 gcaaaccaat gatgatggct ggtatgcaag caacgatcac tttaaaccaa agtgtccacg
    53041 tatctttttt ttctaccgtt gatttacttg gagaaaatgg gtttaacttg tgaaaataaa
    53101 gaacgacgac agccaagatc gcccctaatt ggataacgac attgaacatc gacataaatt
    53161 gtgaactggc atccagtttg atgaattcat ctgctaggat caaatgacca gtactactga
    53221 tgggcagcca ttccgttatt ccttcaataa taccgagaat cactgctttg ataatatttg
    53281 caaaaaacat aaattccttc cttcccaatt aagatacatc ttatagtata ccgttatttt
    53341 ttgagaaacc caatctgttt tttaatatta agaaaaactt gagaaataaa gcagataagt
    53401 acacgaagag aaaaaagaag acaaagccct cgttcagact ttgcctttta tgattctctg
    53461 gtaaaaatta aaatatcccc attatagaaa cgagtcgcga ctatgtgttt tcccgttatc
    53521 gatataagac gtatcattga tgccgtggat agaaaatcga ccgtttgcat aagtgacctt
    53581 tgttacgctg ccatttaaaa gcccagcacg aacaggctgt ttaggatcga tcagattaag
    53641 caaaaaggcg atcgtcaaac catgagatac gactaaaaca tttccaccac catttttttc
    53701 gcagtgatgg gcgatatctt caaatcctgt ccatacacgg tctcgcaatg tttcataagt
    53761 ttccgcccac ccagcggtat ctaagttgta tacagcatta gcaatttgtt caaaagaaac
    53821 ttctgtggta aacatttcat catatgtttt aaaattcaat acacgaggga tcacacccca
    53881 catttccatg tcatatccac cttcaagaga cccgaaacac cattctctaa tccttttttc
    53941 aataaaataa ggaatttctt ttcccttaga atgttctcct agaataatac gggcagtttc
    54001 gattgcacga ccactgtcac tagaataagc aaatgaaaag tcgacatttt ttaacccgcg
    54061 acctaaataa tgaatgcctt cttgtccttg ctttgtgagg ggtgtatcac accagccttg
    54121 tactcgttca attgtattga acatcgtttt tccgtgtcgt acgatataaa gtgtagtttc
    54181 tgtcatttta ttctcctcct aagtcgtttt aacgattgaa aaaaggattt gtcctttttt
    54241 cctgttcaat cgtcgttgca tcgccatgtc ctggatagac tgcaaattca tcaggtaata
    54301 caaacagctg ggtccgaata ctatgaagta attgttctaa attccctgta tacagatcgg
    54361 ttctaccgat acttcctcta aacagggcgt cgccagaaac gacaaaatca tcaaaaataa
    54421 agctgacgct tcctatagaa tgccctgggg tagggacgac gcgaaaagac atgtttccta
    54481 aagtataagt tttcatttca aattcatatt ctgcaggcga gacaataaca tcagcaatat
    54541 catcatgtct accgagtcca gaaagattca aaatcggatc acctagccat ttttgctcta
    54601 gtgggctgac ataaactggg atttgataat acgcgcgtaa aggttccact gcaccgatat
    54661 gatcgtagtg agtatgggta agcaaaatcg ctaccggctg ttggtttgtt tttttgattt
    54721 cttgtttgat tcgttccgca tcttctccag ggtcaatgat caaaagtgct tcttcgttgt
    54781 agaccaaata acagttttct tcaatcattc ctgtttttaa tctttcgata tgcatgtgca
    54841 ttcctccttg atagccaaaa cttctttcat ccattagtat aacgcattca ttgctaggaa
    54901 acaaaaacag gtacttattt ctctttactt atctgaatta tcagactata tgaaaagtat
    54961 ttggatattc taaagagaag gaaagtaagt tttcatagca ttttaatatg tccttgttac
    55021 attagattca tccaaaaatc caaattctat atcctatatc cctgcctaat cagcagggtg
    55081 tttttttatg tttggaaaga gagctctttt tgctaaacta ggttttttca ggtaatctta
    55141 gagagaaaga ggtggtggaa atgatcaaac agtcaaaaat atctgtgcgg cacttagtag
    55201 aatttatttt gcgacgtgga agtatcgaca atcgtaaaaa aagtaaccat actgcattag
    55261 aaggagcgag aatccaccgc aagctccaaa aagatgcggg agaaaactat gaaaaagaag
    55321 tctttttaaa aacaacagtt caattagatg aatattacct gatcgttgaa ggccgtgcag
    55381 acggtatttt caaaagagac ggtatttatt atatagatga aatcaaaacc tccgaaccac
    55441 gatttgaaga tttagaacca gaacaagtgg aacttttctt tcatcaagca agggtgtatg
    55501 cctatatcta tagtcaagaa catgatttac aagaaataaa tctgcaattg acttacttcc
    55561 agacggcaga agaagtgatc acgagaaagg ttgaacatca gacaagagag cagttagaaa
    55621 ctttttttaa aaagctgact gaagattata aagaatggct tgtttttcaa gaaaactggc
    55681 gcactgtacg caatgcctct ttgatggctt tgaaattccc ccatgatgag tataggaaag
    55741 gtcagcgtga actagcggta gctgcttata aaacgatccg tacgaaacag aaattgtttg
    55801 tagaagcacc taccggaaca ggaaaaacaa tctctacgtt attccccgct ttgaaagctg
    55861 taggggaaga agagggagaa aagatctttt atctaacggc taaaacaata actagacaag
    55921 tagctgaaga tgctatgaca gcattaaaag atacaggagc tgaagtaaaa agtgtcactt
    55981 tgacggcgaa agacaagatc tgctttttag atgagacgac ctgtaatcca gatcagtgcc
    56041 catatgccaa tggttattac aatcggatta atgaaggttt atgggatctt ttgaatcatg
    56101 agaatcagat cactcgtgaa gtcatcgaaa cttatgcaag aaagcataca ctttgtcctt
    56161 tcgaactatc attagatgtc agtatctggt gtgacgtaat cattggggat tacaattatt
    56221 tatttgatcc aactgtttat ttaagacgtt tttttgaaga tgaaaaaaac gaggactatc
    56281 tgtttttgat tgacgaagca cataatttgg tgaatcgttc ccgagagatg tattcggcag
    56341 aactatctta tgaaaaaacg aaacgaagta aggaagctat accaaaggaa ttcaaaaaac
    56401 ttcatcgccg tttcaacaaa ctattaaaag aatttgattc gatccgagaa atagcaaaag
    56461 aagatcattg ggattatcat catcaaaaag caccggctga atctttagta aaggcaggtt
    56521 atcaattaag cgaaaaaatt aaagaatggc tggcagaatt tcctgagcac ccatcacaag
    56581 aacaattgct tccttattat tttgatttgc ttcatttttt gaaggtcagt gaattttatg
    56641 atgatcacta cgaaacaaca gtcgaaaaaa cgtatcgcga tctgatagta aaagagtttt
    56701 gtattgatcc aagcttgttt cttgagcagt cgttggataa aggaaaaagc agtattcttt
    56761 tttctgctag tttttcacca ctctcttatt atcaggaaac actgggggga caaaaaagtc
    56821 tggcttacaa actgccgagt cctttcccag aaggaaacca gcaagttttc atcgcaaatt
    56881 acctagagac gacttatcga aaaagggaac acagtctggc aaaattagta gagacgattc
    56941 atgcttttgc tgatggaaag gtcgggaatt atttcgtttt ctttccttct tatcaatatt
    57001 tagatcttgc ggtggaagca ttcagacagc aatatcccga cagcaatgtg ctaatccaag
    57061 aaacggacat gaatgaggaa gaacgcgaac gatttttaac aaaatttcaa acaaatcccg
    57121 ctgaaacatt gattggattt tgcgtacttg gcgggatttt ttcagaagga atcgatttga
    57181 gaggggaccg attgatcggc agtatgatcg taggtgtagg tttgccgcag atgaatcatg
    57241 agcaggaact gatcaaatca tattatgatg agaaggaaca ccttggattt gcttacgctt
    57301 atcagcttcc tggaatgaac aaagtcttgc aagctggtgg tcgagtgatt cgtggcatgg
    57361 cggatcaagg aatcgttgta ttggcagatc aacgattttc tagtttttct tatcgacgtt
    57421 tattcccaca acattggcaa acagcaagag aagtacgaag tgttggggaa ttggaagaag
    57481 gaatacagcg tttttggtta tcaaaagaag cagtaaatca aaaaaacgag gaatgacaga
    57541 cgtcagcctc gttttttgat taacgatagt tagtgaattg caactcgatt ggtaagtcaa
    57601 gtttacgtaa taaagcaatc acagcttgta gatcatcacg gttcttacct gtcactctaa
    57661 tctgatcttc ttggatttgt gcttttactt tcagtttttc atttttgata gctgtagtga
    57721 ttttcttcgc gttttcacga tcgattccgt tgaccaaatc ggcagtttgt cgagctttgc
    57781 cacctagagc gtgttctgat tcggaaaaat ggatattttt gattggcaca tttcttttga
    57841 tcagcttacc aaataataca tctttgatct gttcgatttt gaaatcatca tcgctcaata
    57901 caactagatt gctgttctct aatttgattt ctactgtaga atccttgaag tcaaagcgat
    57961 tggacaattc ttttgttgtt tgctgaatcg cattttttac ttcttctgta ttgacttctg
    58021 atacaatgtc gaagctagca tcttttgcca taggaaccct cctgatatac atataaatat
    58081 ttaccataca tctatcttat cataggatta gggcagattc ttacctgttt gctaaaattc
    58141 ttctcatttg agttcgaagt attttatttt aatagataaa ctgttattct tacatttgga
    58201 ataccaaagt aggggagagg tgcttgtgag taaaattatg actgcgtatc ataaaaatga
    58261 aagattaaag aaaatagtgg ttattttgat aaccggattg accgctgcgg tcgctttaaa
    58321 ttattttttg attccagcca atgtcttttc tgcagggatg aatgggatcg ctcaaatcat
    58381 ttcctcgctt ttatatgaat ggtttcatat caagatagac acaggaacat ttatttttct
    58441 tttgaatatc cctgtgtttg tattagggtt tctcaaagta ggaaaacgag caacgatcct
    58501 tagttttgtg aatgttgtat gtgtatcggt gatgacgatg ctgcttccag tcggacagat
    58561 cactgataat attttgatga atgccctagt aggcggtgtt ttattaggtc ttggcgtagg
    58621 attatcttta aaaatgggct ttacaactgg tgggatggat atcatctctc ttgtactttc
    58681 acaaacaact ggaaaaacag tcggaaacta catgctgatt ctaaatggta tcattgtagt
    58741 catcgcaggc ttcttcttta actgggaaag tgccttgtat acgattattt ccatttatgc
    58801 aatgactcaa gtaattgatg ccatccacac gagtcatcaa aaagtaacag caatgatcgt
    58861 taccgtcaag ccagaagagg taacagaaga actagcaaaa cggatggtac gtggaatgac
    58921 attactgccg tctgtcggtg gctattcaaa aaaacaaggg aacatgatca tgatggtcat
    58981 cacccgctac gaaatgtatg atcttgatca gatcgttcat gaaatcgacg acgatgcatt
    59041 tatcaatatt ttaccaactc attcagtatt tggccggttt gcaaatgaaa acgaacaaaa
    59101 aatgtatcga acaactggcg tattgccaga aataaaaaat cgttcacaaa aaaaatgagg
    59161 aatcaggttt attttagaat aaatttaatc tattacatct aataagaaca tttttataaa
    59221 tttaaacgag aatcttactt ctaaattctc atttacaagc agctaaaaaa agagtatgat
    59281 aaataaaaat cgtgttaagg agtgtcgtac gatgagcgaa gcaaaccaag aacggtctgg
    59341 acaagaaata gaactaatca aagaacgtat tcttgctgcc ttggaaatgg tgattgatcc
    59401 tgaattagga atcgacatcg tcaatttagg cttgatttat gatatagaat tcaatcctga
    59461 aaatggagaa acagtcatta agatgactct aacaactatg ggctgtccat tagcagatat
    59521 tttaacagaa tctatccatg acgcattaaa agaagtgcca gaagtaacga aagcagaagt
    59581 caaactagtc tggtatccag catggacgac agataagatg agccgttatg cacggattgc
    59641 gttggggata cgatagaaat cttggctgac tagctatgag aagcaaatag gaaaaacaaa
    59701 aaagcctttt atcctttcta gaagaaaaga gataaaaggt ttctttgttt ataaaatttt
    59761 gtctgctata ttataagtta attaaataaa tgtgttctat tgataaagga cattcaaatc
    59821 cctttccttt tggaatcctt cttttttaca tcagtttgat tttcgtatag tattctttct
    59881 catcttcacg ataaatcgca gccccatttt tttgttgtgc tttaagactg ccaagatttt
    59941 ttttatttac agacaaaagt gcctcatcaa tgccgatttt ttttgcctct tgtagtacta
    60001 atgctaatcc ttgtgtggca tatccttttt tacgataatt aggggagatg ccgtaaccaa
    60061 tatggccagc gccgtttctt aatgtttcgt tcagtttatg gcgcaaatta aaaattccta
    60121 catactcatg ttcatcactt tctaaaataa agacggttgc gggaacgtaa ctctcaggaa
    60181 gatcgattcc taaagacgtt ttttcgcatt tttttacata atctaaaaat tcagcaaatg
    60241 tatagccata agcattgttg ataaatccac tctcatttgg tccaaagcta gtttgtagct
    60301 gataagcttt ctttaaatct gcttcccata gtttgatcag tttcatcgta ttcacctctt
    60361 taagtaacct tattcatttg ttttctacta tagcactatt gttgtaaata gccatgccta
    60421 tttttattta ttgtgtcata tcgtattaag aatcatcgaa caatacaaat atgaattaca
    60481 ataaaaaaat tatttgaagt tcgttcattc gttacttatg atctctagaa gaaagaaaac
    60541 gtgaattact tcagcgtcta gaattccaga aggatagtaa tcaacagaag aaataatcaa
    60601 tatccaaatg aaagaagata gctgaatcga gaaaaagagg aaaaaagaaa tgaagaaaaa
    60661 aataaaatag ttactaatcc cagagtcgtt cgtttaacat tgaaatcgat catatggata
    60721 aaacagattt tttcaccaac cctcaataga tgatggagtt gctcggatga attatttgtg
    60781 tagatggcat aagggagggc gtacagaaat gaaaaagaac aaaaatatgt tcttgacaaa
    60841 aaacacatgt aatcctaaac taaaagatgt gatgcggcta tggcggaatt ggcagacgcg
    60901 ctagcttcag gcgctagtga gggtgacctc gtggaagttc gagtcttctt agccgcataa
    60961 tttgtttttt tataaagaga aggtacacct ggagcgatga gaaagaaaat aacatattat
    61021 ctaaaagaat ataggaaaaa ttttaaacaa cggaaccaga ttcaaataaa tcaaaaaaaa
    61081 tttgtgtgaa ggtgggacag tttaaaacct aatagattga ttcaaatagt agatttatag
    61141 attctgatac aagaaatact ggcaaacgat agttaaggaa taaagactaa acttaaactc
    61201 aaaaagaggt catgcgaaag aataaatcgc atgacctctt actatatttt gaatgtgttt
    61261 aaacgttcaa ataatgaatc ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct
    61321 tcccaagaac cataacgtga agtaacatat tgttctgcta ctctttcttg gtttgcagca
    61381 gagtagtcac catttaagta agaagaatct aattggtaac ggccgatgta acgtccgttc
    61441 gttgctgtgt aagaaccgct tgattctttt tgtgcgatcc attcttttgc agagcttgta
    61501 cttgctggag tagcagcagt ttctgttaca gcaggagcag gttgtgtttc agcaacaggc
    61561 gctgcttgtg tttccgtttg aaccgtttct tgtacaacag gttgttcagc ttgaacaggt
    61621 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
    61681 ccttctgttg ggattgttaa ttgttgtcct gagtagatca ggttaatatc agaaattgaa
    61741 tttgattctg caatggcatt gatcaaggag ttatcaccaa cgtatttttg agaaatcgtt
    61801 gatagagtat cacctgattg aacggtataa gcttcgtccg catgagcagt cgttcccatg
    61861 aagaatgcag cgccggcagc caaagttgtt ccaaaaagta aagttttaag tgatgtcatg
    61921 aaaaatctcc tttattcgtt aaaaagtttt atttgttgtg ttcaacgagg aatactttaa
    61981 cacgtgaaaa tatttcatag gtaaaagaag catgacaatt tatgacaaat ccacgttttt
    62041 tgtaacagaa tgatgttcgc tcgatgtgaa aaatgatgag gaatttcaca taatgcagaa
    62101 ataatgggat tagaacaggt gttctgatga gaaaagaaat gtataaataa cttgaaagaa
    62161 attgcttttg ttcatcattt tatttacata ttgcgtatat tttttgtatt tttgcttgag
    62221 atgttacaat aaaagaaaaa ggaggcggtt ccaatgagta aaagatacca gaacatcatg
    62281 gtagcagttg acggatcaag acaatcgatc aaagcgttcg ctgaagccct tgacttagca
    62341 aaggacaatg aagcccactt atttatcgtg tcaatcatca acaaagttga gctgacccat
    62401 agtgcctatg ccttttcaaa aatctatgca gatgaaaaac aaagaacaga agtagccatg
    62461 ctgaaaaaaa tcaatgatgc caaagagtac ggcattgctg agatccacgc aatagttgaa
    62521 actggagatc caagaaattt gattgccaat gtcatccctg gacaagaaaa tattgatttg
    62581 atcgttatgg gagcgacagg aaaaggtgct atacaacaag cattagttgg atcgaccgct
    62641 tcttatgtcg tatcacatgc cccttgcagt gtgttggtcg tgaagtaatg agataaggga
    62701 ggaatagaaa atggttcgtc ctcaagaagt ccgtgcgcca aaagaaaaaa tagaaatttt
    62761 agcaattatt gaagacggca cacaaacaaa gaagggatac tcgatcgctt tagtaaagtg
    62821 gaaaggaaaa aaaggtgttg ctctgagatg ggatggagat aaccaacaag ataaaggatt
    62881 ccctgtcaca gctaacggtt atcatccagc gtggtttgtt ctgcctgata aatttatgga
    62941 attatacagc tatgattatg caagagccgc agcatcaatc aaagcattag ataaattaac
    63001 agaagagcag aatgaaatgg aggaaaaata aaaaagacga accaatcagc ctttagaaaa
    63061 ctgattagtt cgaggagtaa aaatgaaaaa aggttatttg ggatgaaact agaataatgc
    63121 gtataactta aataaaactt aaagcaataa aatggcaaga gggtgaggca aatggaagca
    63181 ggaaaagtat tacaggaagt aaaagcacta tgtgcagata aaaaatatga agaagcgaaa
    63241 agcctgatcg aaaagaacaa agaaaatttg gaagaaaagt acccgatcgc acaagaattt
    63301 atagaattaa aacaaggaac gatccttcac cgattcaaac atttttttgg tataacagaa
    63361 tagaaaaaac gattaaagat cggcagaaat ttgctgcatc tttaatcgtt ttctttttaa
    63421 acatgcgagg gattcaattc tgattcttat ataaaaaata gatccaaata ataagaaaaa
    63481 ttactgaaag cgtcaattcg atgatgtgtg ttattaatac agacgttgtt tcccaatgcc
    63541 taaaagaaaa aatttgataa gtaaaatata cagcaggtaa agaggccagt aaaactgaaa
    63601 acttcttttt tgtccatttt atgtaataga aaagaatgct tatcataata aaaaaaggaa
    63661 tataaaaaac aaattgaccg atcgcgatca tactattgcc tccgataaat aaagtccatg
    63721 atacaaaata acactttgtg cgaaatggcg caagcagttt tgtggattta tccggtaaaa
    63781 tctgtatcga ttttgaaatt agaggagaaa cttgctataa tcaaagtgaa agaaggagtg
    63841 ggattttcat ggatcatgta gtgttagaaa tcgatttgca tttgatcaat aatattcggg
    63901 tgatttacta tgttgtaagt aacagtgtcg aacaacgtgt actgacgaat aaaataaatg
    63961 gtattttagc aaagaaagac gttcaccgat tcaataatgg tgaaggaagt tattatagta
    64021 tcccagtaga aaaaattatt tatacgacag tcaaagtcag agaagatcta gataccaaaa
    64081 aagcatatga acctattttt acaacttatt gattgacagc agatggagga aaaaaatgaa
    64141 tccatatgaa caacttattc aagatttgat cgatggaaaa atcgaaaaga ttgatgtaaa
    64201 aagagaagaa ttaatggctt ttcgagaagc gtggctgaag ttggagaatc gtaaatatgt
    64261 ggtgggaaaa gcctcactga acggcaatgt gacttatcat tacgatccga cacgtttatt
    64321 ttagtatgaa gtattcagaa aagaaccaaa ggaggacgat gcagcagcat tgtcctcctt
    64381 tgattttaca aaagtgaaag ttttacttat ttgaatagat ttttaaattt gtttttgaca
    64441 ctgtcgatat ttacatcttt taaaagattt tgggctttct ctttataagg tcctaattgt
    64501 tctttatgtt cttcgataaa tcgttttgcg ccatcgaaat tgccgttctt aatcatttcc
    64561 ttgacttttt gaaataattc ttcttgattc attttgaaca catcctttgc tatcaacatg
    64621 attttttaca tggacaaaac gagaaaaact gatttatcca cacgtcaata atatcaaata
    64681 acgatcattt ttactaaaaa aacactcgat tctcgcttct aaagtaggag aaaaatctcc
    64741 aacttgggac ggatggaatt tctttaactg acccatatac tgaatatata acaaggaggt
    64801 agaaagatga aagtgattaa atatttgatt ggactggcgg cgattagtgg tattttgcta
    64861 tttggactga aatattatac tgaaggaagt tatggagaaa tccctggtat acttgatcag
    64921 ctaaatccat tagttgaaaa aggagaggtc tatgtaaaga cacaaaaacc agaggaagta
    64981 aatgaatatg gtacagcaag gtatgtgcag aaagctgctg atgccaatgg gaaagaacga
    65041 acggttgaat ttaatggtct ttctgtgtta aaagaaaatc attatttgaa aattagcaat
    65101 aaaggagcac acatagaaac gtatgaggaa gttcctagag aaaaagttcc ggagaaagca
    65161 ttagcaatta ttgggtaaat cagtttttgt attaagaata cgcccgattt tattattcat
    65221 aatttaatta tgtaaactaa atacaagtag taaaaataat acaataatcc ccctgcaaaa
    65281 agattgttct tttacagggg gttcattttt tcagcaagca catcaaattg ggatggcagg
    65341 gttcgaacct acaattgctt gattcagagt caagtgcctt accagtttgg ctacatccca
    65401 tttattcaac aaaataatca tagcactttc ctaaaaaaac gcaagttatt tttcttgatc
    65461 ggatagttgt tgctttactc gtaaaaggcg gcgtactagt ttcttttcta atccagcagg
    65521 aagggcaatc agttctgcta ctgacaagac ggtgtccaat aactgatatt ctttgacagg
    65581 ttcattcata atatgataga tgtttgctaa acgttcaata tattcaaagc gagtatactc
    65641 tgttcgagca attagaaatt tcaataaatc ggtggctttc tcgtactctt gatctgcgta
    65701 ataaacaaga gattgacgat acaatttgat ttccagttca ttgagggtag caggtctgcg
    65761 acgctcgttc cactcattaa tagagacttt accttgaaca tttcccttgc agtcacggac
    65821 aaaaataatc ggaatttcat ttggatccat ttttttatgt gtgaattttt ccattatcta
    65881 cgctcccttc aaaggtttgg tatcttgagt ataagggatt tttgtttgaa ttactagctt
    65941 ttctccaaag ttagcatctg tccacccatt gcagtgaaaa tcgccgaaaa atcgttgatc
    66001 ggtactgctc gattttttac accataggga tcgttgacat aaatattttc tttgtcatat
    66061 ccagtgacta cacaagcatg aacaagagga gtgactctta cttctccatt atttgtggtc
    66121 caagtcataa aatcagtttc atctgggact tcaaaattga tagtcacaga agtccagaca
    66181 ggatttcctt tagatagtgc ttttttgata tcatcgagtt gagcatcggt ttttggaaaa
    66241 gtgtgataat ccgtttggac gatttgctga gcaagggagg caactggggg aacatagacg
    66301 accatagccc agtctccgcc actgatatca ccaacaaatc cttcattagg atcgccatga
    66361 gtcccatctg cattgaaaac agggacgtaa tccaattgct cagctaactg gtttttagtt
    66421 gtttcataac cataaaaaga taaaagcata gaaagcgaag tgacttcaca gccattttct
    66481 aaagaaggag aatcaaactg gctctctaat ggaacatcta gtaaaacttc tgctttctct
    66541 tttgtctttt tcgttttttg tgttgaactt ttttgtgcag tgttcattgt atgttctgga
    66601 gaatggatat gtaggtaata acttgtaccg cttaataaaa gagcagctgt gccccctaga
    66661 aacaatagtt ttttgaacgt ttttttcttc atttgaaatc tcccttttca gctgtttatc
    66721 tttcctatta taaatgtatt tttattggaa atggaagtat tatattttaa tatgtaggaa
    66781 ataattagtt tttaaggaat atgttttaat tttttgaaaa aaattcttct aaatacaaca
    66841 gctgttttaa ttgtgaaaac gaaaacagta cgctataatg cctgtaaggt tgatttttaa
    66901 ctgtaaataa aaatcaatga gttaaatctc caaagaaata gcagtggtaa tgtggagaaa
    66961 agttccagca gtttaggagg attttgatga cggaagaaat gattaattta ggagaacaat
    67021 atgcatgcaa accaattggt tttacaaaaa cggtcattgg tgaagttgta tcaaaaatga
    67081 caaactgcgc cgtggtcaaa gttgcccaat gtgctgcaga ggaccaagaa ttattagatg
    67141 aaaaagcaag catggttgtt gcaaaatatg atacatttga ataaagaaaa caattggacg
    67201 tgcggcgtgc tgcaagtcca atttctttga gaaggtaaat cagtattctt ttcctatttt
    67261 ctggagaaga aaaagcgagg cgagttgtca tggtaacatt ctttacggtt ctattgtttt
    67321 tattcgttgt tgtgatgttt ttatttcttt ggctggttcg aaaagaaatt atttatcgga
    67381 ctgtgcgaaa tcgatgggtc tacttggtga tacccttctt agtgtcacta gtcatttggt
    67441 atacactcat cagtcatcct acagcagatg aattggcaaa agggatcttg tcagcaatga
    67501 tttttattag ttttttgttg gacagtagag gaatcacaga agaaggactt gttttgaata
    67561 gctttgacaa aaaaggtgtt cctttttctg agatcaataa aattgtatta tatcagccaa
    67621 aaggttctaa aatcgtaaaa atgaattttt tccgaaacgg atggcgagga ccgatgctga
    67681 aattttctgc atcattagaa gaattagttc catttttgtc ccaacgctta aacgatgagg
    67741 cagagattga tattatgatt gatccagaat aaaaacagag gttgcgacaa gatgtttgtc
    67801 acaacctctg ttttttattt caaataaaat tatccaaata cgcggaagcg atcggcatcg
    67861 ttcatgtatt ctttttcccc agccggtgtt tctggtgaac cgaatactaa ttgcgaacgt
    67921 agtttccagt ttgaagggat attccattct tttgcaactg cttcgtcaat aactgggttg
    67981 taatgttgca agtttgcgcc caatccttta tcaactaatg ctacccaagt attagctgtt
    68041 gcgattccgt ttgattgttc tgaccaatct gggaagttgt ctgcatataa ttcaaattgt
    68101 tcttgtagac cttttaccac gtcagtatct ttgaagaata atactgtacc ataaccattt
    68161 ttgaaaccag ctaatttgtt ttgtgtattt gggaaagctt ctgctggagt caatgggcga
    68221 agtgcttctt cagtgatttc ccaaagtttt tcatgtgcat ctccgaaaag gataactgcg
    68281 cgtgttgatt gagcattgaa agcagttggg ctttctttaa ttgcttcttt gatcaaagtt
    68341 gttaattctt cattagaaag tgaaacatta cgtcctagat gatagattga acggcggttt
    68401 ttcaaagtgt cagtaaaagt tgtcataata aaatttctcc tttgatttgc gttttgcatt
    68461 tttttaattg attacgaaat gaagtatagc accttacaaa aagtaagtaa acagatatgc
    68521 tcacaaaaaa taagcgaagt tcaaaaaaca attttaataa gaaaaatcgt tatgtaagga
    68581 ttttcttagt tatcgctttc attgttgtgt tttaggaatt tttgcttatt tgaaaaaaac
    68641 agctgatata taattattaa atctctatct tctttttttt atgatattgc cattaaaaat
    68701 tacttgcttt tatagaaaat tagtttatat ttcttaaata agttttttag ttaaattttt
    68761 cggtaaagat gtataatatt ttctgaaaat ttaaagaatt ggggtataga ttatggaaaa
    68821 acagcaagac aagggcttct tcgggcagcc gaagggattg tcaactttgt tcttcactga
    68881 gatgtgggaa cgatttagtt attacggtat gcgtgcatta cttatctatt atatgtacga
    68941 tacagttgcc aacgggggac ttggattacc acgagcaaca gcattagcta tcatgtcgat
    69001 ctacggatct ttgatctaca tgtctagtat cgtcggcgga tgggtatctg accgagtgtt
    69061 aggagcaaaa cgcactgtat tcttcggtgg agttttcatt atgcttggcc acatcgtctt
    69121 ggctttacca tttggtgtaa gtacattgtt tgtttcgatg gcattgatta ttatcggaac
    69181 cgcctttttg aaaccaaatg tttctggcat ggtcggacat ctttattcaa agactgactt
    69241 gcgaagagat gcaggatttt cgatttttta catgggtgtc aacttagggg cattattagc
    69301 accactaatc gttggtacaa tcggtcaaga atataactat catttagggt tctctttagc
    69361 tgctattggg atgttctttg gattattaca atacatcatc caaggacgca agacattaga
    69421 cggagtgggt acaacaccgc cgaatccttt gacaactgaa gaacgtaaag gattcatccg
    69481 taacttgatc atcgcacttg ttgttatcgc tgcagtattc ggcggggcac aactaactgg
    69541 tcatctgaca attgactttt ttgtcaatac aatcagtgta ttggggatta tcttaccgtt
    69601 gtattacttt atcaaaatgc tgacttcaaa agatgtaact gctgaagaaa aaccaagagt
    69661 atgggcttat atcccattat tccttgcagc aatcattttc tggtcattac aagaacaagg
    69721 ttcatctatc ttagctttat ttgctagtga acgtacacaa tctacgctat ttggtgtaac
    69781 gatccaagca agctggtatc aatcattgaa tccaattttc atctttattc tgacaccagt
    69841 attcgtaacg atgtggacga aacttggtga acgtcaacca tctacagtag tgaaattctc
    69901 tcttggactt ttatttgcag gattatcgta cttgctattg atgttgccgg gtatgcttta
    69961 tggtacaagt ggaaaagtaa gtccattgtg gttgttggga agtttcttca tcgtggttat
    70021 tgctgaattg tgtctttcac cagttggttt gtctgtgaca acaaaattag caccacgagc
    70081 atttgaatca caaacaatcg ctatttggtt cttagctgat gcttcttcac aagcaatcaa
    70141 tgcacaaatt gcacgcttct acacaccggg aacagaatct gcttacttcg gtattatcgg
    70201 tgcagtagca atcgtcggtg gtatcttgct atttactgtg aaagaaccaa tcaaaaaact
    70261 aatgggctct attcattaat caatgaacat aaaaacagga attgcttatt tgcagcagtt
    70321 cc
//
LOCUS       DAPBBG010000009        61330 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPBBG010000009 DAPBBG010000000
VERSION     DAPBBG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 61330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.009"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(326..553)
                     /locus_tag="RD477_000677"
     CDS             complement(326..553)
                     /locus_tag="RD477_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7633061.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(562..1035)
                     /locus_tag="RD477_000678"
     CDS             complement(562..1035)
                     /locus_tag="RD477_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDT7633062.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            complement(1675..1848)
                     /locus_tag="RD477_000679"
     CDS             complement(1675..1848)
                     /locus_tag="RD477_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633063.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2265..2831
                     /gene="amaP"
                     /locus_tag="RD477_000680"
     CDS             2265..2831
                     /gene="amaP"
                     /locus_tag="RD477_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDT7633064.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2844..3032
                     /locus_tag="RD477_000681"
     CDS             2844..3032
                     /locus_tag="RD477_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDT7633065.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3045..3602
                     /locus_tag="RD477_000682"
     CDS             3045..3602
                     /locus_tag="RD477_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7633066.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3634..3876
                     /locus_tag="RD477_000683"
     CDS             3634..3876
                     /locus_tag="RD477_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7633067.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4217..5431
                     /locus_tag="RD477_000684"
     CDS             4217..5431
                     /locus_tag="RD477_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDT7633068.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5462..6889
                     /locus_tag="RD477_000685"
     CDS             5462..6889
                     /locus_tag="RD477_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7633069.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            6912..7838
                     /gene="glsA"
                     /locus_tag="RD477_000686"
     CDS             6912..7838
                     /gene="glsA"
                     /locus_tag="RD477_000686"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDT7633070.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            8046..9506
                     /locus_tag="RD477_000687"
     CDS             8046..9506
                     /locus_tag="RD477_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633071.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9727..11490
                     /locus_tag="RD477_000688"
     CDS             9727..11490
                     /locus_tag="RD477_000688"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDT7633072.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11574..12032
                     /locus_tag="RD477_000689"
     CDS             11574..12032
                     /locus_tag="RD477_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDT7633073.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            12141..12824
                     /locus_tag="RD477_000690"
     CDS             12141..12824
                     /locus_tag="RD477_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDT7633074.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12971..13585
                     /locus_tag="RD477_000691"
     CDS             12971..13585
                     /locus_tag="RD477_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDT7633075.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13601..13807
                     /locus_tag="RD477_000692"
     CDS             13601..13807
                     /locus_tag="RD477_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633076.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            14348..15478
                     /locus_tag="RD477_000693"
     CDS             14348..15478
                     /locus_tag="RD477_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7633077.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15570..16325
                     /locus_tag="RD477_000694"
     CDS             15570..16325
                     /locus_tag="RD477_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7633078.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16383..18422
                     /locus_tag="RD477_000695"
     CDS             16383..18422
                     /locus_tag="RD477_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDT7633079.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18531..19682)
                     /locus_tag="RD477_000696"
     CDS             complement(18531..19682)
                     /locus_tag="RD477_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDT7633080.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19836..20366)
                     /locus_tag="RD477_000697"
     CDS             complement(19836..20366)
                     /locus_tag="RD477_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDT7633081.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20465..20815)
                     /locus_tag="RD477_000698"
     CDS             complement(20465..20815)
                     /locus_tag="RD477_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDT7633082.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20972..22501)
                     /gene="ahpF"
                     /locus_tag="RD477_000699"
     CDS             complement(20972..22501)
                     /gene="ahpF"
                     /locus_tag="RD477_000699"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDT7633083.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22511..23074)
                     /gene="ahpC"
                     /locus_tag="RD477_000700"
     CDS             complement(22511..23074)
                     /gene="ahpC"
                     /locus_tag="RD477_000700"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDT7633084.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(23218..23880)
                     /locus_tag="RD477_000701"
     CDS             complement(23218..23880)
                     /locus_tag="RD477_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7633085.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            24064..25710
                     /locus_tag="RD477_000702"
     CDS             24064..25710
                     /locus_tag="RD477_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDT7633086.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25701..26783
                     /gene="uxuA"
                     /locus_tag="RD477_000703"
     CDS             25701..26783
                     /gene="uxuA"
                     /locus_tag="RD477_000703"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDT7633087.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26891..28444)
                     /locus_tag="RD477_000704"
                     /pseudo
     CDS             complement(26891..28444)
                     /locus_tag="RD477_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            28592..29733
                     /locus_tag="RD477_000705"
                     /pseudo
     CDS             28592..29733
                     /locus_tag="RD477_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29859..30938)
                     /locus_tag="RD477_000706"
     CDS             complement(29859..30938)
                     /locus_tag="RD477_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7633088.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(31041..31481)
                     /locus_tag="RD477_000707"
     CDS             complement(31041..31481)
                     /locus_tag="RD477_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7633089.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31538..31948)
                     /locus_tag="RD477_000708"
     CDS             complement(31538..31948)
                     /locus_tag="RD477_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDT7633090.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(32128..32562)
                     /locus_tag="RD477_000709"
     CDS             complement(32128..32562)
                     /locus_tag="RD477_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7633091.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32662..33510)
                     /locus_tag="RD477_000710"
     CDS             complement(32662..33510)
                     /locus_tag="RD477_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7633092.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33899..35665
                     /locus_tag="RD477_000711"
     CDS             33899..35665
                     /locus_tag="RD477_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDT7633093.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35767..37026
                     /locus_tag="RD477_000712"
     CDS             35767..37026
                     /locus_tag="RD477_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7633094.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            37173..38474
                     /locus_tag="RD477_000713"
     CDS             37173..38474
                     /locus_tag="RD477_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633095.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38477..39328
                     /locus_tag="RD477_000714"
     CDS             38477..39328
                     /locus_tag="RD477_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633096.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            39341..40156
                     /locus_tag="RD477_000715"
     CDS             39341..40156
                     /locus_tag="RD477_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDT7633097.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            40307..41473
                     /locus_tag="RD477_000716"
     CDS             40307..41473
                     /locus_tag="RD477_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7633098.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41703..42350
                     /locus_tag="RD477_000717"
     CDS             41703..42350
                     /locus_tag="RD477_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDT7633099.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            42434..43378
                     /locus_tag="RD477_000718"
     CDS             42434..43378
                     /locus_tag="RD477_000718"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDT7633100.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43513..44964
                     /locus_tag="RD477_000719"
     CDS             43513..44964
                     /locus_tag="RD477_000719"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDT7633101.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            44985..45821
                     /locus_tag="RD477_000720"
     CDS             44985..45821
                     /locus_tag="RD477_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDT7633102.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            45821..46480
                     /locus_tag="RD477_000721"
     CDS             45821..46480
                     /locus_tag="RD477_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7633103.1"
                     /translation="MYFPLIDLSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46606..47295
                     /locus_tag="RD477_000722"
     CDS             46606..47295
                     /locus_tag="RD477_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633104.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            47301..48257
                     /locus_tag="RD477_000723"
     CDS             47301..48257
                     /locus_tag="RD477_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDT7633105.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            48301..48813
                     /locus_tag="RD477_000724"
     CDS             48301..48813
                     /locus_tag="RD477_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7633106.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(49069..49617)
                     /locus_tag="RD477_000725"
     CDS             complement(49069..49617)
                     /locus_tag="RD477_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7633107.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49706..50887)
                     /locus_tag="RD477_000726"
     CDS             complement(49706..50887)
                     /locus_tag="RD477_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDT7633108.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(51123..52631)
                     /locus_tag="RD477_000727"
     CDS             complement(51123..52631)
                     /locus_tag="RD477_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDT7633109.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52790..52981
                     /locus_tag="RD477_000728"
     CDS             52790..52981
                     /locus_tag="RD477_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633110.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            53184..54101
                     /locus_tag="RD477_000729"
     CDS             53184..54101
                     /locus_tag="RD477_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDT7633111.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            54094..55167
                     /locus_tag="RD477_000730"
     CDS             54094..55167
                     /locus_tag="RD477_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633112.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            55315..55833
                     /locus_tag="RD477_000731"
     CDS             55315..55833
                     /locus_tag="RD477_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633113.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            55814..56206
                     /locus_tag="RD477_000732"
     CDS             55814..56206
                     /locus_tag="RD477_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDT7633114.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            56282..57217
                     /locus_tag="RD477_000733"
     CDS             56282..57217
                     /locus_tag="RD477_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDT7633115.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            57350..58129
                     /locus_tag="RD477_000734"
     CDS             57350..58129
                     /locus_tag="RD477_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7633116.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            58122..58439
                     /locus_tag="RD477_000735"
     CDS             58122..58439
                     /locus_tag="RD477_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7633117.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58683..60293
                     /locus_tag="RD477_000736"
     CDS             58683..60293
                     /locus_tag="RD477_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7633118.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60450..61298
                     /locus_tag="RD477_000737"
     CDS             60450..61298
                     /locus_tag="RD477_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7633119.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        20397 a        10763 c        11921 g        18249 t
ORIGIN      
        1 acttcaaggt atgaaacaga atggcctgta atactacatg attatttaga ctgctcaaac
       61 tgctatcagt ctggtggaat gaataaagta ttatactaaa agcagatcca actacaacgt
      121 aacaggaaac cagttgtatc aggaaaagtg atagtacatg gagaagaaaa ggcccccttt
      181 gtatgagtca gtacatatct tatttataga taaaacggta ttcaatcgaa ataaaaaatc
      241 tgaataatag acgtaaataa cttctaaaag tctcacacat ctatcattca gattattttt
      301 tatttactgt ttgttctact ttattttatt cttctggtat agtattcgag tcatttttat
      361 aggtcaacaa atcacctggt tgacactcca aaacttggca gattttttcg agagtggaaa
      421 aacggatggc ttttgctttt ccatttttta gaatagagat gtttgccatc gtaatgccca
      481 ctttctcact caattcagtc acactcattt ttttttgagc taatactaca tctaaattaa
      541 cgataattgc catgtcgcac ctcacactgt caaatcatga tctgtcttaa gtaaaatcgc
      601 atgttcaagc agtttttcca gcacgcttgt taatgtgtat actgcaaaag gaatgaatac
      661 gacagcgatt cctaccaaca tcaaacctgg agcatcgtct aaatccgcta ctttcataaa
      721 tttaggaagt aagccaaaaa aggcgaaacc aataaacaaa atggttcgtt cgattttttt
      781 tagtatgttt aagctttgag tggaaaaagc ttggttatct tcgatttttt tcaatagttt
      841 caataagaaa taagcaataa cgatccctag gagaagggca aagtaaatga cagaaacaaa
      901 taatgtggtc tgccaatcaa aattgaatcc tttttcgctt gtagcgatct gagtataaaa
      961 aagtacgccg aacaaaataa atacggcact tatacctaaa acgacaaatt ttaatagcaa
     1021 tgttttaaat ttcattttta tccctctttt atctttgata gctaaaatat agcagaataa
     1081 ttattgtaaa acaactattt tttatcgaaa aacaataaaa atatatcgtt tataaaaaat
     1141 gatagagtat gaaggtgtgg agtgcattta acagcttttt ttgattgtaa gaaacattta
     1201 aaaccagtta ttttagattc tatgtgtgtc tcttctataa tgctagttta gatgcctggg
     1261 attatattgg acaatacagt tggaggattt tttattatct agcgaagaga agtgtaagtt
     1321 aacagaactg tatgagaaaa aagacaacta cgtagtagct aaccgattca ttaatttttt
     1381 caagttcatg tttatttacg tatcagaatt tttcttattc aatggtttgg tgtaatcacc
     1441 acttatttgt cctttattgc ttatacaatg ttatacaaaa ggaaaatcaa gcattgacgc
     1501 atggggtggt actatctagc tgaaatagct taataagcgc aagtagatgc attggattag
     1561 tttataaatc aaaagtgtta gaggtattaa gaagaagtag aaaaataaaa tggattgccg
     1621 aataaaaagt accataaatc ctaaaacatg tgccaacaga tagatggcac atgtttattt
     1681 ttttcgataa ttttgtaaca aaaatacgaa gatgaaaaag aatagagatt cccataatgt
     1741 agtagtccaa ctgaattctt tactgatacc aaataccata tttcctagat ttgatacaat
     1801 caaatagcca atcgttatga tcaatgaata gtacaatctt ttcatcatag gacatacctc
     1861 atttttattt ataagaatag tataaaccaa agaagtgaat aagaaaaatg ataatataaa
     1921 aataaagtaa gaacgcatta aagtaactaa agttatttat tgtatttttt aattctccga
     1981 aaaaatttaa taaataaata gaatcttgta ataataactg agcataatct ttgcaatttt
     2041 taatcttgaa taatatcctt taagtatagc cagcaatcaa aagtaaatag agaataagta
     2101 agcaattgtt atttcagtat cagactggca ataaaacgcc gttatgatcc agcaatgttt
     2161 ggtagcgctt tatttactga tgctatgcta actgttcgct atctatactg tgattgtcgg
     2221 tagagttatt tacagggtaa aaataagttt ataggaggtg gaaaatgaat aaggggttaa
     2281 aaataatcgg tgttatcatg agtttactgc tactttcagt atttctagcg attgcgttga
     2341 tcaatgcgcc gtatattatg cctgaacaat tggaacgatt tcgcttcttt actttaacga
     2401 attactacat gcaacaatat attttctggg cagctgtagt atttgccgtt cttgctatta
     2461 tcatcttgct agtcgttctt ttctatccaa agtcaagagg aactttcgtt atgaaacggg
     2521 aagatggcaa actaacaatc gacaaaaaag cgatcgaagg gttggtacgt tcccatttac
     2581 acgaagaaga gttcattcat tcacctaaag tacggatacg ttcaacgaaa aatcgaatcc
     2641 atatccatgt caacggagac ttgaaacgaa cttcttcatt agtagggaaa acaggtgtat
     2701 tgatgcaaga tatcgaagaa gaagtaacta aagtactcgg aacaaaagaa acggtcaaag
     2761 tggcagtcac atacagcggt tatgaagaac aagaagatac aagagactac aagcactctc
     2821 gagtggaata ggagggccaa ataatgaaag aactattttc agcatacaag ctccctatta
     2881 tctttggggg tcttggacta ctgctagctg ttttatggtt aacgattgga tttttcaaga
     2941 cattgttgat cataatcatg acagcaattg gcgcaggaat cggtttttat ttgaaacagt
     3001 caggaatagt tgatcaattt tttaaatcat aggaggaaac aaagatggaa aacaaaaaca
     3061 tgaatacaac aaatggatca gttttggcaa atgaaaaagg agaaaaccgc atgatgaatg
     3121 gtgaattgac ttatgaagac aaagtcatcc aaaaaatcat cggttatgca ttgaaagatg
     3181 tagatggtct attgacatta gatggcggat ttttctcaaa tatggctgaa aaattagtca
     3241 acacagataa cgtaacagca ggtatcaata cagaagtagg taagaaacaa gtagctgtgg
     3301 atctagatgt gatcgtagaa tacggtaaag acatcgaaga tatttacaac caaatcaaag
     3361 aattgatcag tactgaagta aacaatatga cacatttaga tgttatcgaa gtgaatgtga
     3421 atgttgcaga catcaaaaca caagaagaat accaaaaaga tcaagaaaca gtacaagaca
     3481 aagtaacaga agcagcaaaa tccacaggac aatttgcttc acgccaaaca gataaagcaa
     3541 aatctgctgt aggcaaaggt gcacaaaaag tcaaagaaaa taacgaacca cgtgtacaat
     3601 aagaagtaag aagtcagtta ggaggaattt aaaatgggat ttatttgggc attaatcgtc
     3661 ggcggtatca ttggagcaat tgcaggagca atcacgaata aaggatcatc aatggggatc
     3721 atcgcaaaca tcattgctgg tttagtaggt tcagcaattg gacaagcttt actaggaact
     3781 tggggaccaa caattgcagg aatggctatc attccatcat tgatcggtgc aatcatttta
     3841 gtattagtcg tttcattctt tgcacgtaaa ttataagcaa attgaagaaa aacttatgtt
     3901 gaaatgaaga acaaatttga gaaccgtagc gacactcgct gcggttcttt tttatatatt
     3961 ttatatgaag tattctttat tccttatgaa aaaatatctt taaaaataaa aaaagacgaa
     4021 aataaacggt tttctataaa aagaagaccg tttatttttt gtctttattt gtgtgaaaaa
     4081 cgaaaaagag cacaacacat caagctttta ttaattgtct gtcttttata aatgcaagta
     4141 ttaattcaaa aaaacagaag aaaaaaacaa ctacaccttc ttttatactc gtaatcgtag
     4201 aaaggaggcg aaaaaaatga atacattacc tatcaataca ggagatacag cattcatgat
     4261 tttatgtaca gcaatggttt gcctgatgac tcctggtcta gctttttttt acggcggatt
     4321 agcacggaag aataatatac taacgatcat gtctcaatct tttctctccg ttgggataac
     4381 aacgattatg tgggtatttg gtggattcgg attggccttt ggacgggata tcggcggtgt
     4441 tattggaaac ccggcagatt tctttttgat gagacatgtt acaagcttcc ccaacatgtt
     4501 tcatggtgca acgattccat ttttaatgtt ctttctttac caactgatgt ttatcgtgat
     4561 taccgttcct ttgatgacag gagcttttgc agggagattg aatttaaaag gctatattat
     4621 acttgttatt ttttggaatc tgctgattta tttccctgtt tgccactgga tatggggtgg
     4681 cggatttttg gatcagatgg gatttagaga tttcgctggt ggagcagtca tccacacgac
     4741 agctggattc ggctctttag catgtatcct cacacttggt caaagaaaga ttcctttagg
     4801 agaaagaagt cagcatagca gtcttatggc agctgctatc ggtacaggac tgctttggtt
     4861 tggctggttc ggttttaatt ccggtggtgc acttcgagca gatgaacaag cagtcaatgc
     4921 atttggttca acattcatcg ctcttgcatt tgcgatgatt acttggctga tcattgcaaa
     4981 agtaaaagga aatggctttg attttgtcga cgtactaaca ggatccgttg ctggattggc
     5041 agccattacg ccttgtgccg gttatgtaga agcaaaaagt gccatgctga ttgggatcat
     5101 tgcgggtatc gtctgtcatg ctgctgttga ttttcgtaaa aagaaacaat gggatgatgc
     5161 acttgatgtg tggggtgttc atggcatggg cggattcaca ggtacgattc tgatagggat
     5221 ctttgcatct aacagccatt tactaacgaa tccaagcagc tggtacttct tagggattca
     5281 agtgattggt gtcatcatta ctgccgtgta tgcctatgtg ctgacaacag tgattttaaa
     5341 atcggcgatg cactttacaa cgatcactac tacaaaagaa gaacaagaag aaggattgga
     5401 ctttttcttc tttggcaaca aactcaattg aaacaaattc aaatagaaaa gaggaagatt
     5461 catgcaacaa agcaaaacga aaattcctgc aaaaaaatta acgttctttg gctttttggc
     5521 tatgaccatc tcaatggtcg tttcattata tgaatatccc acattcgcga cctcaggctt
     5581 cagcttagta tttttcttgt tattaggcgg tttgctatgg tttatccctg tggcattgtg
     5641 cgcagcagag atggcaacgg tgaaaggatg ggaaaaaggc ggtgtctata cttgggtcag
     5701 tcgcacattg ggaaaacgat tcggttttgc cgcaatcttt ttccaatggt ttgagataac
     5761 agttggctac ttgaccatgc tgtatttctt aactggtgca ttatcctatg caacgggtat
     5821 tggtgctata caaaacaata aatatctgaa attggcaatc ctattgatta ttttctgggc
     5881 tattttactc tcacagttaa aaggaaccaa atacacttca ttgattgctc ggatcggttt
     5941 tgtagctggt atcttgttgc cagcattagt cttgtttggc ttagggatcc attatgtcgc
     6001 ttcaggcgct ccactacaaa cgatactgtc tatgaaaaca ttgattcctg atttttcgaa
     6061 attgcctacg ctggttgttt ttgtttcctt tattttagca tatatgggcg tagaaacatc
     6121 tgccagtcat gcaaatgaaa tggaaaaccc taaaaaggat tatccattag caatgtttgt
     6181 attggttatt ctggcaatca ttttagatac ttttggcgga ttgaccgtcg ctactacagt
     6241 acctcaacat ggtttgtctc ttaatacagg ggtgattcaa tcgttagaat tcctattaag
     6301 acacattgat ccaaatcttg catggattgc gaaaattctt gcgattcttg tttgtttagg
     6361 tgtcatcgga gaaattgctt cttgggtaac ctctccttca aaagcacttc acgtagcagc
     6421 agaagaagga ttacttcctg aatattttgc aaaagaaaac acacacggtg ttcctgctca
     6481 tttgatgatc gcaaatggga tcgttgcaac aatctgggca gccgtcttta cattaagcgg
     6541 cggggggaat aatctttcat ttcttgcagc gatgtcattg actgttgtaa tctacctgat
     6601 gttgtacttt atgtttttcg cgggctattt gaagctggtt tttaaagatg acagtcttga
     6661 aagagcctat caggttccag gcggaaaagt tgtaaaagta atcgttgctt gtttaggtct
     6721 tttgacttca ctttttgctt tttgtatctc atttgttcca cctgctagta tcgcagctgg
     6781 acagcatggg atttatgaaa cgattctgat tgtttcttat cttatcgtct tagttattcc
     6841 atttacgatc tacgcatgcc gtcataaatt ccagcatgca gaaaaataaa aaatgaaagc
     6901 tggttattca aatggaagta aaagaattag aacaattgat tggaaaaaat ttccactatt
     6961 atcaaacagg acaagtggcg gattacattc cagcattagc tgaagtggat cccaaacaat
     7021 taggtatggc gatttatgat ttggatacaa aacaactgat tgagggagga gacgccgatg
     7081 tgcgctttgc catcgaaagc atgtcaaaag ttcccgtatt gttattagca atcaaagaca
     7141 acggaataga aaaagtcttt caaacaatca acacggaacc aaccggtttt gccttcaatt
     7201 ctatcatgaa tatggaaatc aatcatcgca aacatccaat gaatcctttt gttaatgcag
     7261 gagcgattgc aacaacctcg ttgattgccg gtaaagatgc agatgaaaaa tttgagcgaa
     7321 tcttgtcatt tatgaaagag atctgtgatg atcctaatat cacattgaat gaagaaatct
     7381 atcattctga atcaagaaca ggtgatatca atcgctctct tgcttactat atgaaaggta
     7441 atcaaatgat cgaaggagac gtaccggaga tactcgatgc ttatttcaga caatgctccc
     7501 tcaatgtcac cgctacaggc atagcaaaat tagctgctgt gctggcaaat aaaggaattg
     7561 ctccttggaa tggcaaacaa atcattactg aagaaagtgc tacaatcgtc aaatcaatta
     7621 tgacaactgc tggtttgtat gatgaatcag gagagttttc cgtccatgtt ggtgtacctg
     7681 caaaaagcgg tgtaggcggc gggttgatgg cagctgtgcc aaatcgttac ggcataggtg
     7741 tcttcagccc ggcactagat ccatttggta acagcgcagc tggtatccaa ttgttgaaag
     7801 atgtagtcaa agaactagac gctgacatct ttgaataacc attcttgaaa gaggctgggg
     7861 taaaagtcca aacggctcaa agctacctac aatgaggctg aaattgtgag cttgtttgac
     7921 ttttgtccca gtttcttttt ttattggttt ttgaaagggc ttttctaaat aaaaaggaaa
     7981 caaaaatacg atttacagat atgattaaat aaaatcataa gaaaaactgg aggcgtgtat
     8041 caggaatgct ggaaaattac atcgagcgaa atatatttag aaaagtgtat ctgtgtgaac
     8101 aattatttga attccaagaa atagacatcg aacaaacagc tatttctcta agggtgacta
     8161 caccaacgat ccttcacgat ctagaaagtc tagccgagtg tctagaatat tgtataaaag
     8221 aacaagtaag agagaagcat aagtataaac tcgtattcaa acatggtatt gctctttctg
     8281 agctcacaca gtttctgtac ggtcaatctt attttttaaa gtttttaagt tattatttta
     8341 atggtatatt cacgagtaca gaactggctg atcttgaatt tatttctttg agcaaagtat
     8401 atacaatcaa aaaaatcgta ttggattttt ttaaagaaaa tgattatttg aaaaataaag
     8461 aaatcattat cccagaattt gactcgcgga acatcttgct agctttagta cgttatgtca
     8521 attgggaagg ctatgaaaat aaaaatccgt atgtagaaga agcttgtaac gagttgattg
     8581 attatgtaga gactcatttt tttaaaagaa gatattcgga agatgaaaag tcctttatca
     8641 tacgaggaat agagatcgca atcggaagaa aggcatttcc ccttggactg aaagaagaag
     8701 ataagaaaag cgcagaaacg aagccgttat ttcaattagt ctgtcaagga ttggaagagc
     8761 aaaaaggaat tctagactta caagaagaag atgtctatta tattttttct ttgttcaaca
     8821 gtagaaatta taccaatgag aacatggagc tgttaagaaa agatttcgaa gttgtttata
     8881 aaaattttgt tctcaatgac ctatcactga atgaattgat tttacagatt attagtatcg
     8941 ctgaaatcga cgatgcagaa aattttattt tcaagcaatc ctttttacaa tttgtccgta
     9001 cgttgtgggc agatggacaa gtatttttac cggaaaggat ctatcttcta tcagaaaaag
     9061 aacaacttct gtacggaaaa attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa
     9121 tccaactaag atggaatgaa aatttcattc gaaaattcgt caaaagtctt tctttgatca
     9181 acagtgaaca agcggggaga caggaaatag aaatttttgt tgtttcagaa agttcagtca
     9241 aacaattttt ttatcgaacc caatttcatt tgtatgcgga agaaggcatc gctgaaataa
     9301 atcctatttt atttcgttca ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa
     9361 gagtggtttt gtgcgatgct gctagctatc aaaatggatt agaaactgaa aagacaaaga
     9421 tctatccaat ctcattaaaa ggtgcaaaca tccatttgta tcatattgct caggaacttc
     9481 acctattaaa aaaacatatt tcttgatgag accttaatta ggagaccttc tacaaagctt
     9541 tttaattgtc tgtagaaggt ttttatttga gtcctgattc gtgcgaagat ctacttatat
     9601 aacaagcttt ctctgaaatt tttttaaaat gctattgact tagagtcaac tctaagtagt
     9661 agaatagata aagtgtaaca aatctatttt cattaaaaga agatagtacg gaggagagaa
     9721 tgtattatga gtacagtatt acatgtattg gatgcaatca atagccccgc agatgtcaaa
     9781 aaattatctg ttgaggaaat gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa
     9841 aaggacagcg atgtaggtgg gcacgttggt ccgaatctag gtgtagtaga attaacgatt
     9901 gcgtttcatt atgtatttga ttcaccaaag gataaaatcg tttgggatgt ttcccatcag
     9961 tcttatggtc ataagctttt aactggtcga aaaaatggtt ttgaaaaagg acattaccgt
    10021 gaagtgagcg gatattccaa ccaacatgaa agtaaacatg actattttac agtggggcac
    10081 acatctacat ctatcagctt agcaacaggt ttagctaaag cgagagatct gaagaaagaa
    10141 tcaggaaatg tggtagcatt tatcggtgac ggctccctaa gtggtggctt agcttttgaa
    10201 ggattgaaca atggtggtgt tttacaatcc aatctaatcg tattagtaaa cgataatgag
    10261 atgtctattg atgagaatca tggcagttta tataaaaact tagctgagct tcgtcaaacc
    10321 aacggtaaaa gcagcaataa cctatttaaa gcaatgggat ttgattatcg ttacgtggat
    10381 aatggaaacg atctggaaac ggtgatccgt ttatttgaag aagtaaaaga tattgatcat
    10441 ccgattgtac ttcacgtcca tacggaaaaa ggtcgcggtt atcagccggc aatcgataat
    10501 aaaatgaaat atcattggca tgtaccattt gatctgtcca ccggggaggc aaagcaagta
    10561 acttctggag aaacatttaa tatgatcatc atggattatt tggataagaa aatgcaaaca
    10621 agtacagctc ctattgccgc agtcaatgca gcgataccag gcgcctttgg actgaaaaca
    10681 ttccaagaaa aacatccaga tcattatttt gacgtgggga tcgctgaaca gcactctatc
    10741 actttcgctt ctgctttggc ttctcaggga gtacgtccag tcgtcttcca atcaagtact
    10801 ttcttgcaac gggcatatga tcaattatca catgatttag cgatcaacga gaatccagtg
    10861 attattttag tcggtggcgg aacgatctca gctagtgacc gaacacacca aggaacattt
    10921 gacattcctt acttatcaag tatcccgaac atcaaatatc ttgctccagc aacaaaagag
    10981 gatatgatct ctatgctaga ttgggcgttc gaacaaacag cgcagcctgt agcaatccga
    11041 attcctacac atggtgtaga acatggtaca gctatcgcaa atgattatag tattccagcc
    11101 tatcaaacag taaataatgg aggaaaagta gcaattttag ctttaggcgg ttttcttgaa
    11161 ttaggaaaag aaacagtaaa agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat
    11221 ccaggaacga tcacagaaaa tgacgtagaa acattagata aattaaaaga aacacacgaa
    11281 ttagtcatca cattagaaga tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt
    11341 tattatggtg attcttcaat gaacgtatta aattttggtg cagagaaaga attcacggat
    11401 agcgttcctg tagggcagct ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa
    11461 gatagtctgc gtgtcttgaa tattcaataa ttcattatgc tcaccatcca gataattgtt
    11521 tatactagta tctggatggt tttttcatta taaaaagcaa aggggtggat gttatgtatc
    11581 agcaagtaga agaatatgtc aaaaagttcc aagaaaaata tcctagttat caatcagaaa
    11641 aattaggttt cttttcattt ggggatacag aaaaaatggc agacgagttg agcgaactag
    11701 ttgtaaaaaa gataaagact ggtacatcaa gtggttacga tctgtataaa caagagaatg
    11761 agcctttacc aaaagttggt caattagacg tggtcctgaa tggaaaaaat gaaccagttt
    11821 gtatcatcaa aaatataaat gtgaccatct tgccttttag tgaagtcacg aaagagcatg
    11881 cttggaaaga aggagaaggg gatcggacat tagcttattg gcggaaagtc catgctgagt
    11941 tttttaaaca tgcttataaa gaagcgttga acatagaatt tacagaagag aagctggtcg
    12001 tatacgaaga atttgaagta attttctttt agtaagaaag gtctgctgaa agaaatagct
    12061 cattattgtc tcctgaatag aaaagggtta catttttata tgaagctgta tttttatata
    12121 acattactgg aggagatatt gtggatacac tggaacaaat caaaggcgga gtcgtcgttt
    12181 cttgtcaagc tttggaaaac gagccgctac acagttcttt tattatgggg agaatggctt
    12241 tggctgcaaa agaaggcgga gcagttggta ttcgagcaaa tacgacagaa gatatcaatg
    12301 aaatcaagcg aatcgttgat ttaccggtaa tagggattat caaaagagat tacgatgggt
    12361 cagaagtgtt tattactgcg acagaaaaag aagtggaaga acttttggag acgcaagcag
    12421 aaatcattgc actggatgca acttctcgta aacgtccgac agaaaaaaca actgctgaac
    12481 ttgtccaaat gattcacgaa cacggtcggt tagctatggc agatatttcg acttttgagg
    12541 aagcgcttca agcgcaagaa gatggttttg atcttttgtc tacgacttta gcaggctata
    12601 cagaatattc acgaaagacg cagacacctg attttgattt attgaatcaa atcatagaac
    12661 aagtcgatat cccagtaatt atggaaggac atactgactc tcctgaacaa gtagagaaag
    12721 catatgaatt aggtgctttt tcagtcgtag ttggatctat tatcacccgt ccgcaattga
    12781 tcactaaaag atatgtcgaa gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac
    12841 agattcttga caaaaaagaa aaattgatag atagtttaaa gcagattcta agaagatgaa
    12901 aaaagcatta cttttcattt tgtcttcaag atctgctttt taacgaattt tatagaaaga
    12961 aggcgaaacc atgtttgaac ggagtccttc agaattgaca gaacgtgaaa attacaagtt
    13021 gctgattggt tcagtcatcc ctagaccagt agcagtagta acgactaaat cagcaaaaga
    13081 tatagtgaat atcgcaccct tcagttactt caatatcgtc agttcgaatc gacccatctt
    13141 atccttggcg atccagagaa aacaagatgc agtaaaagac acagcaaaga atattcttga
    13201 gacaagagaa gctgttatcc atatccttga tgaggacaac gtagaagaag taaataaaac
    13261 agcagccaat ctgcctaatg aggaaagcga actgtacctg actgacttga ctttaactga
    13321 ttcagtaatc atttcagtac cagcattgac agaagcaaaa gttcgttttg aaacatcctt
    13381 atatgaacat gtcgagatac ggaataaaaa taaagtaaca gctgatttat ttttgctaga
    13441 agtacagaaa tatcatttaa atgaaacagt ctatgatgat aagacaggaa ggatcgatcc
    13501 aagaaaattg aatgctgtaa gccgtttggc aggaaatgat tatgcagcaa taggagaaat
    13561 tttcacgatt gcacgtcccg aataaaaagg agcactagtt atgttaaaaa taagctataa
    13621 accatcgaca gattcaaaag aaatgaaaaa agaatatgaa acagtgaatg attttctgca
    13681 agggcagtac ttagaagttc cgccattgca agaccatttt gtcgttacta ctgttacttt
    13741 agatggtaag gaaatagaaa tgccagatca aacgattagc ggattgttta attactttaa
    13801 caaataaata tttattccga aaagtgaaaa aacatataaa aaaacacata aaactaaaaa
    13861 attatcaaag aatcctggtg caactgaaag aatttgcaac gggattcttt caacttattt
    13921 gttatttttt tctaaaaaat attaaagatt gttaaatttc actatttcat ttcaaattcc
    13981 attgcctaat cattaggaaa agtctatcat gaactcgtaa atagaatata gtaataacct
    14041 tgttaggtga ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa
    14101 tgggtaaaac agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta
    14161 cgttgtatag tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa
    14221 aaggtttctg accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc
    14281 tcctcacctg acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg
    14341 gtggattttg ggtccttatt tgactttgtc catgatcggt cttttagaag tatacagtgc
    14401 cagttcttat cgtttattgc aagccgatga aaatacaaaa agtctattat tgcgtcaact
    14461 aattttcata tttttgagtt ggagcgtgat cttcttagct cgttcagtca aactacacta
    14521 tttacttcac cctaaaatag caggatacgg tttagcctta tcgattttct ttttagtatt
    14581 agtaagaata gggatattcg gtgtcactgt caacggcgca caacgttgga tctctctgtt
    14641 tggcattcaa ttccagcctt ctgaactggc aaatcttttt ttgatttttt atttaagctg
    14701 gttttttcgt gacggaaata gtagcccaaa agatctaaaa aaaccattcc tgattacagt
    14761 aggtataact tttctgattt tatttcagcc aaagattgct ggagcattga tgatcctttc
    14821 gattgcgtgg gtcatatttt gggcagcggc ggttccattt aaaaaaggga tctatctaat
    14881 cgttactttt tctgcattgc tgattggagc agcaggcggg gtattatatt taggaaataa
    14941 aggttggctt ccacagatgt ttaatcatgc ttatgaaagg atcgcgactt taagagattc
    15001 ttttatcgat agtcatggtg cgggctatca aatgacccat tcgttttatg cactatataa
    15061 tggtggcata tttgggcggg gattaggaaa tagtatcaca aaaaaagggt atcttccaga
    15121 gacagaaaca gattttattt tttcgattat tacagaagag cttgggctaa tcggtgcatt
    15181 gtgtgtgctt ttcttattgt tctcattatg catgagaatc ttttgtttaa gcagtcgctg
    15241 taaaaatcag caagctggat tgtttctttt aggattcgga actttgctat tcgttcagac
    15301 gataatgaac gtgggaagta tcgcggggct gatgccaatg actggtgtgc cattgccatt
    15361 tgttagctac ggaggaacaa gttatctgat cctttcttta ggaatcggaa tcacgttgaa
    15421 tatttcttca aagatacagg ccgaagagtt ggcgctctat cgacctgaaa aacaataaag
    15481 agaaactgag aaactctcag tttgaattat cataaagtac aatcttttat ttttcaagcg
    15541 agaggttctt ttagaaagag gaattcgcaa tgaaagtatc tcaaaaaatc atattatcta
    15601 ttttgttact gcttacatta gtagttggct atttttcgat tgagtttatc catggatttt
    15661 cgtctgcaaa acagagttca actgtgaaaa aggtagagcc gaaaagtatc cctaccacac
    15721 taaatgtggc tttgattggt tcagatgctc gttcgaaaga cgaaaacggt cgctcggatt
    15781 cacttctggt tgcacaatac aaccagaaaa cacaacaagc aaaactgatc tctatcatga
    15841 gagattcata tgtcgatatt ccaggttacg gaatgaataa gataaatgca gcttactctt
    15901 atgggggagt tgatttatta aatcaaacat taaaggaaaa tttcaaattt gaagctccct
    15961 attatgcaag tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg
    16021 gagtcaagat cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag
    16081 agcaagtgat ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag
    16141 gagacttcgg gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga
    16201 aagacgtaac ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc
    16261 aaacgaacct tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacatat
    16321 gttagaataa acaggtataa atagtgaaat aaaggaatga caagcaagag aaggaggaaa
    16381 aaatgaaaag aagtgacaag cacggcaaaa atcgaacagg cgcttatatt gccggcgcag
    16441 tgatcttaat agcagctgcg ggtggcggtt atttttacta ccagcactat caagaaaccc
    16501 aagcagtaga agctggagaa aagacagttg agcaatttgt ccaagctttg aacaagggag
    16561 attataacaa agctgcagaa atgacatcaa aaaaggcggc aaataaaagt gcattatctg
    16621 aaaaagagat cttagataaa taccagaata tatacggtgc tgctgatgtc aaaggacttc
    16681 agatatcgaa tctaaaagta gataaaaaag acgattctac ttacagtttt tcatataaag
    16741 caaagatgaa tacctcatta ggtgaattga aagatctttc ttataaagga acattagaca
    16801 gaaatgatgg tcaaaccacg atcaactggc aacccaactt ggtttttcca gaaatggaag
    16861 gaaatgacaa agtaagcctg accacacaag aagcagcaag agggaatatt atagatcgaa
    16921 atggtgaacc attagcaaca acaggcaaac taaaacaatt aggagtcgtt ccaagtaaac
    16981 ttggagatgg aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc ttcgacttaa
    17041 cagaagatgc tatcaatcag gcgatttcac aaagctgggt acaacccgat tactttgtcc
    17101 cattgaaaat cattgatgga gcaacgccag aacttccagc tggagctacc attcaagaag
    17161 tagacggcag atattatcct ttgggtgaag cagctgctca gctgattggt tacgtgggag
    17221 atatcacagc agaagatatt gataagaatc cagaattaag cagtaatgga aaaatcggtc
    17281 gctcaggttt ggagatggct tttgataagg atcttcgtgg gacaacaggt gggaaattaa
    17341 gcattacaga tgcagacggt gtagagaaaa aagttctgat cgaacatgaa gttcaaaatg
    17401 gcaaagatat caaattgaca atcgatgcga aggcacaaaa aacagctttt gacagtctag
    17461 gaggaaaagc tggatcaact gttgcgacaa cgccaaaaac cggtgatctt cttgcgcttg
    17521 ctagctctcc aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa gaagattaca
    17581 aagcttatga agaaaatcct gaacatccat tcatcagccg atttgcgaca ggttatgctc
    17641 ctggatctac gtttaaaatg attacagcag caatcggtct cgacaacggc actatcgatc
    17701 caaatgaagt gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt
    17761 atcaagcaac gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct
    17821 attccgataa tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta
    17881 caggcttaga taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag
    17941 cacaaatttc taatgaagat agctttaact cagatatctt gctagctgat actggatatg
    18001 gacagggcga acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca
    18061 acaatggcac acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga
    18121 atgtcatcag cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag
    18181 atgtaaatgg tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg
    18241 gtacagcgga aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg
    18301 ctttcaatct tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagaagatg
    18361 atgattcagc aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat
    18421 aaaaaattgc tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt
    18481 caaataaacg gttttcacga tgtctctttc acaacttctt tttgatttac tcacacttca
    18541 taaacttttt ttgtaaaata tttgagaaca agtggagaaa taagtgtact taataaaatc
    18601 acgataacta aaggtgaata gtactgtggt tcgatcaatt gactttgctg tccaatttgt
    18661 aaaatgatca aagccatttc tccacgcgaa atcataccag caccgaccat caatgaacta
    18721 tgttgagaaa aaccggcaat ctgagcacca aaataacctc caagcaattt ggtcaaaatc
    18781 gctacaagcg tcaatataga aataaagagt agttgttcac taaagtcaga aaaatcaact
    18841 tctaatccta cactgacaaa aaatactggt ataaatactg catatcctaa agcctctaca
    18901 ttgtggtata cttcttcttt aacctttgtc tgactgacag ctatcccagc aaaaaaagca
    18961 ccaatgacag agctaagccc aacaagatcc gctacgtaag acattcctaa acaaataact
    19021 agcgatacga tgataataga agaatttgcg tacacttttt ctgcaaatga aagtaggaaa
    19081 ggcgcaatcc atttgactaa taagaaaata aaaacaaaat aaattagctg ttcaatcaaa
    19141 acaagcggta aagaagcatt gctagaagct tcccccgtta gaaaagatag gctgaaactt
    19201 agaactaaaa cgactaaaat gtcatctacc acagaagcac ccaaaattgt tgatccttct
    19261 tttgtattca ctacgtttag ttcttttaat acttctactg aaatactgac agaagttgcc
    19321 gcaagaataa tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc tttgcctgtc
    19381 aaccagccaa aaacaaccgg gaagatgatc ccaagcaaag caacgaacat accaggtcgg
    19441 aaatattttt tcaaaagaga taaatcactt tccagaccag ctaaaaacat taataagatc
    19501 acaccaatct ccgaaaagtc atggactaga atatcaggtt tgacccaatt caatccagct
    19561 tggcctagca aaacaccaac aagtaattgt ccaatcactg ctgggatacc taatctccgc
    19621 gagatatgtg agccaatcgt tgttgaaatc aatatcaggc ataaaatacc aataaattcc
    19681 attctcatca ttcctttgta taaaatatag cccgaactta gcataacata cattattgaa
    19741 aaaataggaa aatatactca caaaaaaacc aacaatccaa aagatttttt tggattgttg
    19801 gtttttatac ggtctatttt aataactaaa aatgattaac gtatagtcat ttttcttatt
    19861 ctgatgaaat tgataatctc ctggatgccc atgaaaatca aaataaagcc gaatatttgg
    19921 aatagaagca tcaaactttt gaatggattg aacaataaga cgaatccgcc aatcaacaac
    19981 agaatactac tataaacact ccaactttta gcttttgtat tcatgctgaa gaacaactgg
    20041 aacaagctgt tgatgataac agctaatcca agaagaatcg gcaggataga aacgattgca
    20101 ggtgcaaaag catagacgat cagagctaag atcaggaaaa aaagaccact gaacagaccg
    20161 atactccaag aacctgtttt ccttttaaaa cggtaagcct caaaaagatt caacaaccca
    20221 agcaaaacaa gataggcggt gatcagataa ccgataaaat gaaagacagc agaagggttt
    20281 agcacaatcg caatcccaga aataatataa acagcagctc ttaataaggc gtatttttga
    20341 atattttgtt taatcgtctc aaacatataa gaaaacctcc tttgacttat tttaacgtga
    20401 actagtcatt ttaccaaatt aaatgttaca aaatgagcga aggagaagaa tatctctgga
    20461 aaaatcaact tctgaggatc ttttccattt ttttgccttt tgctagctca tcgatcattt
    20521 tatctaaata acgaatttct ttcatcaagt tatcctcgat gtcttctact cgtacaccac
    20581 aaacaacacc tgtaatcaag gaacgtgaag gattggggac aggggcttcc gcaaaaaatt
    20641 gttcgaaagt cacttgtttt tgtagttgat attctagttc tgtttgggaa taacccgtca
    20701 gccaacaaat gatttcatct acttcctctt ttatacgatc ttttctctcc gcttttgcta
    20761 catacatagg ataaacgctt gcaaaacttg tcgtaaaaat acgattttgc gccataatca
    20821 gtcacctttc tttttttcaa attatttact acttatagta tataagaaaa agagaagaaa
    20881 aagcgattat tcaaaaaaac aaaaaggatt gtgacaaaat tcttgtcacc atcctttttt
    20941 ccgctctctg actttttaca atcttcatag ctcaagtgcg catcagataa tcgaatgctc
    21001 ctaaagctgc agtagctcct gaacccattg aaacaatgat ttgtttgtat ggactatctg
    21061 tacaatcacc agctgcgaaa acgccgggga tattcgttgc gccatttttc tcggtcacta
    21121 tttcatttct tggattcatt tcgatcgttc catctagcca atccgtatta gggagtaagc
    21181 cgactagaat gaatactcct gaaacagcta gtgtatgcat ctccatcgta cggcgatcga
    21241 tataggagag agattctacc tgattcgtac cgctgatttc tttcgtcgct gcatgtttga
    21301 taacagtgac attcgcaaga gagactagtt tatcctgtaa gatttgatct gcttttaatt
    21361 ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc agccaaatcg attgctgctt
    21421 caacaccaga attaccacca ccgataacag caatctcttt tcctttaaat aaagggccgt
    21481 cacagtgtgg gcaataagca attcctttat ttttaaactc tttttcgcct ggtacattga
    21541 tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt tgctttaagt gatataccgt
    21601 tagctaagcc aatattaata aaatgttgct ctttatttaa ggaaacggcc tgttgattca
    21661 tcataatgtc gatagggtaa ctacgcacat gactttctac ttgctgcatc aattttggac
    21721 cttctgtgta agaggtacca atcatatttt caatccctag tgtttcaacg acttgtccgc
    21781 cgaaagaatc agcgacaagt cctgtacgga ttcctttacg tgctgcatag atagctgcac
    21841 tactagctgc tggtccaccg ccaatcacta atacatcata aggttctttt tcaaacaact
    21901 cttcttcaat caaagggcca gtgatttttt caacaatttg ttcgatcgtc atgcgtccgc
    21961 tactaaattc ttctccattt aaaaacacag ttggcactgc catgattttt ttatcatcga
    22021 tttctttttg gaacatccca ccttcaatca tcgtatgagt aatatttgga tttaatacag
    22081 ctaatatatt caatgcctgt acaacgtcgg ggcaattatg acaagtcaag ctaacataac
    22141 tgtcaaaatg caatgtttgt gttatttttt tgattttaag actcaaagat tcactgattt
    22201 tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa agaggaaaat tcatgaccga
    22261 gggggatacc agcaaaaacg attccacttt ctttgccaac tggattgata gaaaaactag
    22321 gtgtgcgcat cgaatctctt ttttcccaag agattttagg agacatttct gctatttcct
    22381 tcgtaaattc agctagtttt tgactatttt cactattatc caaactaaca gaaaaaacaa
    22441 taggagattc aatcagtgct aaatattgtt cgagattttg tttagtttct ttgtctaaca
    22501 tatccaccac ctaaatcttt ccaacaagat caaaacttgg tttcaatgtt tcgccacttt
    22561 ctttccattt tgccggacaa acttcacctg ggtgggtacg tacatattga gcagcgcgaa
    22621 tcttatcaat cagtgcacta gcgtcacgac cgattccatc tgcattgatt tccatagctt
    22681 gaacgattcc gtctggatca ataataaatg ttcctcgctg agctaatcct tgctcttcat
    22741 ccaaaacatc aaaaatacga gaaatctgat gagacggatc accgatcatc acgtattcga
    22801 ttttaccgat agcatctgat gtatcatgcc atgctttgtg agtaaagtgg gtatcggtag
    22861 agcaagaaaa gacttcaaca cctaaagatt tcaatgtact gtattgttct tgtaagtctt
    22921 ctaattcagt aggacaaaca aaagtaaaat cagctggata gaagcaaaga atactccagt
    22981 tccccataag atctttttca ctgacttcta aaaattctcc ttgatgataa gcatgtgcat
    23041 taaattcttc gatttttttc ccaatcaatg acatagtaaa acctccaata ataattattt
    23101 atttataatt attgtaattt agttggagtt gcaaagtcaa tggatatttt gttatttaat
    23161 aaagattact caaaaacgaa ggttaattat aaaaatagaa gaggtcgcaa gaaaatgcta
    23221 ctgctggaaa tactcgggat aacgttgagt aacagtttcc tgttctttca gcatcatgtg
    23281 taagtgcaat cccgtttgaa atcgagcttc ttctggtttt ttatcaacta aagccttgag
    23341 taaatcataa tgttgttcca tgatcgtttc ccatggaagc ccctgaatgg tcactcgtaa
    23401 ccagcggaaa cgttctaaat gggtattatg gtcatctaac cagttccaaa cttcttttct
    23461 tcctgcaatt tcgaaacaag cttcatgaaa taaattatct gcacggaaaa aagcgcgttc
    23521 gtcatttatt ttgactgctt tttcttgttc ttctaaaatc gtttctagca ctattcgtgt
    23581 ttgtgtgtcc aatttggaac aacattcaat catgatttgc tcttccaact gttctcggac
    23641 aaagcgagca ttttctgcag aaacaagatt aattttggaa acatacgtac cactttgagg
    23701 tatcgtatac acgagttctt gctgacgtag ttgaatcaat gcttcgcgaa tgggagtccg
    23761 gccgatagca agatgttcct ccagtgcctt ttcagatatt ttccttccag gttctaagtc
    23821 agcatagata atttgtttac gaatcgcttg ataagcttgt gattgcaagc tgcttttcat
    23881 ctttattcct ccaaaatcat ttctatgaac gtagtataac agaagaggtc tgaaaaacaa
    23941 tctggtatgt cacattaact agtaaatttg tataaatagt aaaattttgg ttgacatagc
    24001 gatttttatt gcataaaatt cacttgaata caaaaactaa tatgttagtt taggagggga
    24061 aaaatgattg atttaaacaa ctggaggaac tttatccctg aattatctca aaaagaagta
    24121 gaaatacctc attttgaccg tgcatcatta agagaaaatg gacaaaaaaa tcctgtctgg
    24181 atccatttcg gtggcggaaa tctttatcga ggatttcatg cagaaatcgc tcaacgttta
    24241 gcggatctag gcgaattgtc cactggtgtc attgtttgtg aaacattcga tgaagaaata
    24301 attgaaaggg cttaccaaga ttttgatgat ttgattcttg aagtggtcat gccaagcagt
    24361 ggagagttcg tcaaacgtat attagctgcg acagctgaca gtctgtattg tcatccagat
    24421 tgttctgaac actttgataa gatgattcga cactttgaaa atcctagttt acaattagtt
    24481 acttttgcta ttactgaaaa aggttatcga ttgaaagata caaataataa atatatagga
    24541 ccgattgcag tcgatataaa aaatggtcca aagaagccat cacatacaat gagtattctt
    24601 gctgtgctac tttataaacg ttttttatct ggagcttggc cgattgcact tgtctctaca
    24661 gataattttt cacaaaacgg tagtcaatta cgtacttctt tgctaacgat cgcaaaagaa
    24721 tggcaaaagg aaggttatgt acaaaataca tttatccaat acttatcgaa tgaagaaatt
    24781 gttgcgtttc catggacgat gattgatcgt atcaccccga atccttcctt gtcagttagc
    24841 aaaatgttgg caaaagaggg cttttcaaat atgtcgattt tccaaaccgc aaaaggaaat
    24901 aatattgctc catttgtcaa tacagaggac atccattatt taatcatgga agatcatttt
    24961 cccaatggac gtccgaaatt cgaaaaagct ggggtattct tgactgatcg tgcaacggta
    25021 gataaagcag atacgatgaa ggtgacgact tgccttaatc ctttgcatac gtctttagct
    25081 attatgggtt gtttactggg gtataggagc atagctgagg agatgaaaga tccagattta
    25141 tcgctgctag tttctgaaat cgggtacaat gaaggcctcc cagttgtgga gtctccaggt
    25201 atcattgatc caaagatctt tctggaagaa gtaattactg tgcgcttttc taatccaaat
    25261 attcctaata tgcctcagcg aatcgccacg gatacctctc aaaaggtggc gattcgttat
    25321 ggtgagacga ttaaaaaata tatggaaata gaagttaaag acccgcagac acttgtattc
    25381 ataccattga ctctcgctgc ttggcttcga taccttattg gcattgatga tcaaggaaaa
    25441 ccattcatcc cgagtccaga cccactattg acagaccttc agcataagct ttccgatatt
    25501 caactgggta cgcaagatac atcacatatt catgagaaaa tggctcctat tttgtcaaat
    25561 aaagaaattt ttggtgtaga tctttaccaa gcagagcttg gcagaaaaat agaagaaatg
    25621 ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa cattacagga ttacttacaa
    25681 gaacatggag gaaatttatc atggaaatga cttttagatg gtacggatca caggaagaaa
    25741 agattacctt agatgaaatc agacagattc ctgcggtcaa agggattgta ggcacattga
    25801 tggatatacc agtaggagag gtatggccta gggagaggat caaagaacta aaagaagaaa
    25861 ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat gatgccatta
    25921 aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca atccgaaact
    25981 tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt gactgggtga
    26041 aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt atatctgatg
    26101 atattccaga tgatccaaga gaaatcgtcg cgcgcatcga acattccagc aatgaatttg
    26161 gattgccggg atgggaacct gagcgcttag ctcctatcaa atccttgctt gaagcctatg
    26221 catcagtaga cgaagaaaaa cttcgagaaa attttgccta ttttctccaa tcaatcatcc
    26281 caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg ccatactcga
    26341 tttttggatt agctcgaatc gtaaagaatc gagaagattt agattggttg tgtcgcatag
    26401 tagacagccc ttataacgga atcactcttt gtacagggag tattgcagaa gatccagaaa
    26461 atgatgtata tgggatttta gcagaatttt gccaaagaga tcgaatccca tttgcacatg
    26521 ttcgaaatat taaattttta gctaatcgag atttctatga ggcaccacat aaatccgaat
    26581 acggttcatt agacatgtac cgcatactaa aaacaatgta tgacaacgga tttaacggat
    26641 acatccgtcc tgatcatgga agaatgatct ggaatgagac aggaagacca ggatacggct
    26701 tatatgatcg agcattaggt gctgtttatc taaatggatt atgggaagca atcgaaaaag
    26761 gaaacaaaag taaagataat tagtgaaaaa aactgtaatt ttattatgta aataagaagg
    26821 atgttcaaat tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat
    26881 cttatttggt ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt
    26941 cttttaattc aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg
    27001 tgatgattcc ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaaagggc
    27061 agtcgctgat aacgatgttg cagttctcca taaaatcatt ttgtattttg atcgattcat
    27121 ttgaaaaaat ttgttctatc ttaacgatca gatctttact gatataaaaa tcacgcgtat
    27181 agttcacacc aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa tgtactaggt
    27241 cttcaaccat ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt
    27301 gttctctgaa atccatataa aattgttcaa tggcggtttg gacttctttc aatacgctat
    27361 gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc
    27421 cattataaag tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta
    27481 taaacaaatc aaatacagta caaagggact ccaccatttc tcttgaataa agaacatgag
    27541 gaacgtttgc tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt
    27601 tcgtgataga aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca
    27661 gattaggtcg caaaatatct acagagagat caataaaaaa gtcaatatat tcatactctg
    27721 tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta
    27781 atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa
    27841 aaaaagtatt catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag
    27901 tacttgaaaa gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc
    27961 cagaaaaaga ctttctgcca ttattagaaa aggagataga caaaccgtac tatgctaaaa
    28021 atgcattaat ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac
    28081 tgaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga
    28141 ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt
    28201 caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac
    28261 gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga
    28321 aatgatctgc taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa
    28381 tatctaattt tgtattgctt tttttcttca agataaacgt tttaatcgct tcttgagttt
    28441 gcatgaaaga ggctcctttt tacttaaatt gttaaaactt gaaaatatta ctgttttttt
    28501 cataaattta gacaaaaaaa cagtaaacat ataggtagaa tctctactat cttatttctt
    28561 gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct taatatcaac
    28621 atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag aggagacaga
    28681 aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct ttcaggatga
    28741 attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa
    28801 agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg ttacatttga
    28861 gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta tccaacacaa
    28921 gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac cagcgctttt
    28981 tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt atagtcagcg
    29041 gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg aagaaccaac
    29101 agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc cagacggaga
    29161 acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac cagagccaga
    29221 caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc cagaaccaga
    29281 tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga
    29341 aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc cagaaccaga
    29401 tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa ttcaagagga
    29461 tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg aagagcagct
    29521 gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa
    29581 ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc cggagagtca
    29641 caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac aatgctttct
    29701 gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg tgaacgcatt
    29761 tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga aatctgatat
    29821 ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc tgtttctttt
    29881 ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt aaagatcgga
    29941 atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa gaccacaaat
    30001 gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag cacataagac
    30061 tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg gatgcctcct
    30121 tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc tactgggatc
    30181 agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc
    30241 aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc ttctaatacc
    30301 aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa
    30361 aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc aatcataaaa
    30421 aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat atcctgtaaa
    30481 tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt tcgctcaata
    30541 taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc gtaaaaatta
    30601 ataacggact ggatcatgtc aaatgtttga tgaataagca tcgggatata ctttgtgaat
    30661 gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac acctgtaggg
    30721 attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt aaagataaat
    30781 gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac gacaaataat
    30841 aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac ggtcaaatcg
    30901 acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa tatgaaaata
    30961 agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt tttcctcata
    31021 aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt atctagcatt
    31081 tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag ataagacttg
    31141 atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagc agtcagcgta
    31201 acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt tttttctgct
    31261 aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt tcctagctct
    31321 ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg ggtataggta
    31381 agattcagcg gcttgaggag gggagtgtag tgtctaatga tttctttaga ggcggcatat
    31441 aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc tccgctgttt
    31501 tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact gctttcagag
    31561 taacttctat atttccagct gtagcttttg aatacggaca cactttatgc gctgcctcta
    31621 ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc tctacaccta
    31681 agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca gtagaagccc
    31741 cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat gcactgaaac
    31801 cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca ggagctgcaa
    31861 tattcaattc gaatgtacga tcgggattat gcacttctcc agagcgtccg cctgtgttga
    31921 tcattttcgt agaatacatt tttttcataa aattaccacc tttaattata ttttatacaa
    31981 ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata agataaataa
    32041 gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa aaaatatcag
    32101 agacaaaaaa taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc
    32161 ttcaaaaatt acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct
    32221 gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca
    32281 gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca
    32341 gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag
    32401 catatggaat ctcgtaccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc
    32461 tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa
    32521 actatcattt aatacaaaat cgatttccgt tcctttatgc ataaagatct cctctccttt
    32581 tcagtcgaac agttactaga ttcttttatt ataccagcta aagaaaacgc attcaaaaaa
    32641 atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac
    32701 ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt
    32761 cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt
    32821 atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag
    32881 gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc
    32941 tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa
    33001 ctcttctatt ccaaaaataa acagattaag agaaacaacg aatgcgtctg taaaaaataa
    33061 gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc
    33121 gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc
    33181 aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat
    33241 taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag
    33301 aagagctagt accaacataa cgatattcaa cactaaaata actatagctc ggtcaaagcc
    33361 taaagcagct tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg
    33421 ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta
    33481 tgtccatata aattgtctcg tttctttcat ttattcactt ccttaattca ttaataatac
    33541 tcttatctta ttataactta cttttcacct taagttaact ttaattaata aaaaaattag
    33601 gctagtctaa attattatgt ttcattcttc tagaatatac ttctacgcca aaaactgtta
    33661 aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta
    33721 aatagtacag gaagtagact aaaactttta aaagttttaa gtctatctat ttggcaagtt
    33781 taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc
    33841 ttttgcttct ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat
    33901 ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac
    33961 aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctatg
    34021 tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa
    34081 atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaagtcg
    34141 cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca
    34201 aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc
    34261 ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta
    34321 tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt
    34381 accttgggga agtaaagatc cagaccgaga agactttttc gggggagatt tgcaaggtgt
    34441 gatggatcat ttggattact tgacggacct tggcatcaat ggcatctatt tttgtcccat
    34501 atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc
    34561 ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg
    34621 tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt
    34681 tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt
    34741 agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt
    34801 tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt
    34861 gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt
    34921 tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa
    34981 accagacatc tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga
    35041 tgaattccat gcagtgatga attatgcatt tacagattcg attaaagatt attttgcaaa
    35101 aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag
    35161 agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt
    35221 gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca
    35281 aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc
    35341 ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt
    35401 tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac
    35461 tcagtggcta atagtaaatg atcgtgaaga taagctctat ttcacacgga aactagaggg
    35521 acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa
    35581 tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca
    35641 atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca
    35701 aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac aaaggaggaa
    35761 atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc
    35821 ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa
    35881 ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa
    35941 aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat
    36001 cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca
    36061 gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga
    36121 aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac
    36181 ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca
    36241 gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag
    36301 tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat
    36361 ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat
    36421 atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa
    36481 aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa
    36541 ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa
    36601 gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat
    36661 caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc
    36721 tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg
    36781 gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa
    36841 ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa
    36901 atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa
    36961 acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa
    37021 tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa
    37081 agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt
    37141 tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca
    37201 acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc
    37261 aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc
    37321 tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc
    37381 attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga
    37441 ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca
    37501 aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt
    37561 atctattgac acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag
    37621 ttacattgtt cactgcattt actaattatg acttcaatca tattccacca gccagtttaa
    37681 ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata
    37741 ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa
    37801 tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta
    37861 ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt
    37921 tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa
    37981 accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga
    38041 aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta
    38101 caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa
    38161 atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa
    38221 catttattac acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca
    38281 atggcggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga
    38341 tctataaact tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga
    38401 ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca
    38461 atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc
    38521 tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt
    38581 gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc
    38641 aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg
    38701 gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac
    38761 tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt
    38821 ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt atgtcatggc
    38881 tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata taggcggtgg
    38941 aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa tagatttaga
    39001 tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga ttgtattacc
    39061 gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac cttttgggga
    39121 tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg ctgtcggctt
    39181 acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg ctggggcaat
    39241 tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact ttgttaccgg
    39301 cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat ttccgttaaa
    39361 ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac tattatcatg
    39421 gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc ctgtgactct
    39481 tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg ttcattcgct
    39541 gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca
    39601 agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga
    39661 gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat gggtgatcca
    39721 acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat ctttccaacc
    39781 agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat tttatgccag
    39841 taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc tatttgtcat
    39901 gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa aaagccaact
    39961 ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta tgggaatagg
    40021 ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga tgctaagtgt
    40081 ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa gattcaaaga
    40141 taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa
    40201 aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg attcttttct
    40261 tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac
    40321 tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga
    40381 cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca
    40441 tgaagaaatc ccaatgaaag agattacaag taacgcatct ttggcactag gagaagccta
    40501 tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca
    40561 gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa
    40621 acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt
    40681 ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca atacacctga
    40741 agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat
    40801 caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc
    40861 taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt
    40921 gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga
    40981 ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca
    41041 tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa
    41101 aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat
    41161 caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat
    41221 tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac
    41281 attagaacat tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg
    41341 tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc
    41401 aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat
    41461 gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt
    41521 ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag
    41581 tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag
    41641 aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat
    41701 caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc
    41761 aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt
    41821 cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt
    41881 ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt
    41941 tccaagataa atacccagat actgcaatta ctagtttgga attagatagt gattggggat
    42001 cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg
    42061 ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg
    42121 gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag
    42181 caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag
    42241 atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca
    42301 agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc
    42361 ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga
    42421 aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt
    42481 gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc
    42541 agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga
    42601 accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg
    42661 tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca
    42721 accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa
    42781 aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc
    42841 tggaaaacca caactagctt acgattttga ggccacttat gaaacactga aagaagtatt
    42901 cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt
    42961 tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag
    43021 tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga
    43081 agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc
    43141 tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg
    43201 acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtaccgagct
    43261 tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc
    43321 agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca
    43381 aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat
    43441 tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa
    43501 aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt
    43561 agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg
    43621 aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag
    43681 tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa
    43741 aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca
    43801 tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg
    43861 ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct
    43921 tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca
    43981 ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg
    44041 ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct
    44101 tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg
    44161 gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca
    44221 gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca
    44281 ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc
    44341 cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg
    44401 atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa
    44461 acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc
    44521 caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa
    44581 gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact
    44641 gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg
    44701 gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag
    44761 gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc
    44821 agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc
    44881 agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc
    44941 catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat
    45001 tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta
    45061 atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt
    45121 ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg
    45181 tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca
    45241 acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt
    45301 ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat
    45361 ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta
    45421 aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg
    45481 gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa
    45541 atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt
    45601 taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt
    45661 ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga
    45721 tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag
    45781 tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ctctaattga
    45841 tttatccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg
    45901 gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg ttgcttttga cagttttagg
    45961 tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg
    46021 cattcccgca ctagtcctgc tgtttctgtt atattttggg ttaccttatc aattaagtgc
    46081 cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta
    46141 tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt
    46201 ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc
    46261 ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac
    46321 agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc
    46381 tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca
    46441 acagtattta gaaaaacgtt ctatgcatca gtcaatctga aaaatagaaa tggtgctagc
    46501 gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca
    46561 tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta
    46621 accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta
    46681 gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa
    46741 tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc
    46801 tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta
    46861 tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac
    46921 acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag
    46981 aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg
    47041 gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaac agatcaattg
    47101 tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat
    47161 gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc
    47221 gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca
    47281 gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat
    47341 tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg
    47401 aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc
    47461 atcgaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg
    47521 tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag
    47581 aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact
    47641 acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac
    47701 cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt
    47761 ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg
    47821 actatctttt tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta
    47881 gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg
    47941 atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag
    48001 accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc
    48061 atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa
    48121 aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta
    48181 gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat
    48241 atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg
    48301 atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc
    48361 gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca
    48421 aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat
    48481 caaattgctg gaacagctgc attagtctca agcgtggatc ccgtttacac aaaaatagat
    48541 ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa
    48601 agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc
    48661 agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag
    48721 accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc
    48781 gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa
    48841 gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg
    48901 ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt
    48961 agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag
    49021 cttttgtcac gacctctcgg cttatcctac gagtattatt cgatacattt atatcaataa
    49081 tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag
    49141 tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct
    49201 ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt
    49261 agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa
    49321 accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc
    49381 tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt
    49441 gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc
    49501 aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc
    49561 tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt
    49621 catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt
    49681 aaaagtaact ttgctttttt gtatattata ccagcaagcc agtcacaata gcacttaaaa
    49741 agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt
    49801 tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa
    49861 atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat
    49921 ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac
    49981 cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt
    50041 aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg
    50101 caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat
    50161 caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca
    50221 gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc
    50281 caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag
    50341 atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt
    50401 tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa
    50461 tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc
    50521 ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa
    50581 tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat
    50641 ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa
    50701 acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac
    50761 caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt
    50821 tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat
    50881 aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa
    50941 tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt
    51001 agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata
    51061 aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt
    51121 ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct
    51181 agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct
    51241 ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg
    51301 attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc
    51361 tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct
    51421 gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat
    51481 gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa
    51541 ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc
    51601 acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact
    51661 gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg
    51721 atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga
    51781 ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata
    51841 atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg
    51901 taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct
    51961 tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac
    52021 ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt caaggaagta
    52081 gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca
    52141 atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag
    52201 tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag
    52261 actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat taataagcca
    52321 taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat cccataatca
    52381 ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga
    52441 tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt
    52501 ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta aatataattt gtcatagtgg
    52561 ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca
    52621 atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt
    52681 cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt
    52741 ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag
    52801 acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag
    52861 aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca
    52921 gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata
    52981 aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga
    53041 tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct
    53101 tttttataac aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag
    53161 atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag
    53221 acgaactaga ggagacgaat gcggatcagg atacaggaca agaatcgtct tctttttacg
    53281 atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg
    53341 aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga
    53401 atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta
    53461 agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc
    53521 caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg
    53581 atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt
    53641 cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca
    53701 ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga
    53761 ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag
    53821 aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg
    53881 agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc
    53941 agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca
    54001 aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc
    54061 ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg
    54121 acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa
    54181 acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacac
    54241 gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa
    54301 ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt
    54361 ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa
    54421 tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc
    54481 tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg
    54541 aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt
    54601 tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc
    54661 atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg
    54721 gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga
    54781 gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat
    54841 gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt
    54901 ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg
    54961 gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga
    55021 ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg
    55081 atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg
    55141 gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga
    55201 taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg
    55261 tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc
    55321 acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct
    55381 tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac
    55441 ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat
    55501 tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg
    55561 ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt
    55621 ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat
    55681 cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt
    55741 ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat
    55801 tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact
    55861 cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt
    55921 aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa
    55981 agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt
    56041 caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca
    56101 actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa
    56161 attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac
    56221 tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa
    56281 catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat
    56341 tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa
    56401 cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa
    56461 acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt
    56521 tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga
    56581 agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc
    56641 aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat
    56701 caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag
    56761 tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt
    56821 agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt
    56881 gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct
    56941 gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg
    57001 tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt
    57061 agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa
    57121 tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga
    57181 aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc
    57241 aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat
    57301 gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa
    57361 gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat
    57421 accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc
    57481 aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct
    57541 tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta
    57601 tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc
    57661 tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac
    57721 tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg
    57781 ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct
    57841 tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc
    57901 actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt
    57961 ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct
    58021 ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa
    58081 tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc
    58141 tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt
    58201 attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg
    58261 cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg
    58321 cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt
    58381 cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa
    58441 ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga
    58501 actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag
    58561 tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct
    58621 atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt
    58681 aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta
    58741 aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg
    58801 gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc
    58861 ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag
    58921 gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg
    58981 gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga
    59041 ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta
    59101 tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg
    59161 gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt
    59221 acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt
    59281 ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact
    59341 tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg
    59401 cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta
    59461 tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga
    59521 tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga
    59581 cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca
    59641 cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg
    59701 cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca
    59761 cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt
    59821 ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc
    59881 gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg
    59941 gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg
    60001 tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga
    60061 tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg
    60121 ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt
    60181 attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa
    60241 atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact
    60301 aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc
    60361 tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa
    60421 aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg
    60481 catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga
    60541 ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac
    60601 ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg
    60661 gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt
    60721 cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa
    60781 cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg
    60841 ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa
    60901 tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag
    60961 gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga
    61021 cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg
    61081 ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt
    61141 acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa
    61201 aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag
    61261 gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata
    61321 ccaaaaatat
//
LOCUS       DAPBBG010000010        61022 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPBBG010000010 DAPBBG010000000
VERSION     DAPBBG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 61022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.010"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            73..786
                     /locus_tag="RD477_000738"
     CDS             73..786
                     /locus_tag="RD477_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633120.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(901..1911)
                     /locus_tag="RD477_000739"
     CDS             complement(901..1911)
                     /locus_tag="RD477_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDT7633121.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1948..2430)
                     /locus_tag="RD477_000740"
     CDS             complement(1948..2430)
                     /locus_tag="RD477_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDT7633122.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            2580..2948
                     /locus_tag="RD477_000741"
     CDS             2580..2948
                     /locus_tag="RD477_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDT7633123.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3413..3865
                     /locus_tag="RD477_000742"
     CDS             3413..3865
                     /locus_tag="RD477_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633124.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(3989..4840)
                     /locus_tag="RD477_000743"
     CDS             complement(3989..4840)
                     /locus_tag="RD477_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDT7633125.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4853..5638)
                     /locus_tag="RD477_000744"
     CDS             complement(4853..5638)
                     /locus_tag="RD477_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7633126.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(5990..6931)
                     /gene="ribF"
                     /locus_tag="RD477_000745"
     CDS             complement(5990..6931)
                     /gene="ribF"
                     /locus_tag="RD477_000745"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDT7633127.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(6935..7858)
                     /gene="truB"
                     /locus_tag="RD477_000746"
     CDS             complement(6935..7858)
                     /gene="truB"
                     /locus_tag="RD477_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDT7633128.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(8226..10588)
                     /locus_tag="RD477_000747"
                     /pseudo
     CDS             complement(8226..10588)
                     /locus_tag="RD477_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            11036..11209
                     /locus_tag="RD477_000748"
     CDS             11036..11209
                     /locus_tag="RD477_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633129.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(11366..11713)
                     /gene="rbfA"
                     /locus_tag="RD477_000749"
     CDS             complement(11366..11713)
                     /gene="rbfA"
                     /locus_tag="RD477_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDT7633130.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(11740..14046)
                     /gene="infB"
                     /locus_tag="RD477_000750"
     CDS             complement(11740..14046)
                     /gene="infB"
                     /locus_tag="RD477_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDT7633131.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(14059..14370)
                     /locus_tag="RD477_000751"
     CDS             complement(14059..14370)
                     /locus_tag="RD477_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDT7633132.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(14367..14660)
                     /locus_tag="RD477_000752"
     CDS             complement(14367..14660)
                     /locus_tag="RD477_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDT7633133.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(14682..15857)
                     /gene="nusA"
                     /locus_tag="RD477_000753"
     CDS             complement(14682..15857)
                     /gene="nusA"
                     /locus_tag="RD477_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDT7633134.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(15880..16353)
                     /gene="rimP"
                     /locus_tag="RD477_000754"
     CDS             complement(15880..16353)
                     /gene="rimP"
                     /locus_tag="RD477_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDT7633135.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(16498..20856)
                     /locus_tag="RD477_000755"
     CDS             complement(16498..20856)
                     /locus_tag="RD477_000755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDT7633136.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(21068..22777)
                     /locus_tag="RD477_000756"
     CDS             complement(21068..22777)
                     /locus_tag="RD477_000756"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDT7633137.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(22845..24113)
                     /gene="rseP"
                     /locus_tag="RD477_000757"
     CDS             complement(22845..24113)
                     /gene="rseP"
                     /locus_tag="RD477_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDT7633138.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(24274..25074)
                     /locus_tag="RD477_000758"
     CDS             complement(24274..25074)
                     /locus_tag="RD477_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDT7633139.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(25071..25883)
                     /locus_tag="RD477_000759"
     CDS             complement(25071..25883)
                     /locus_tag="RD477_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDT7633140.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(26078..26635)
                     /gene="frr"
                     /locus_tag="RD477_000760"
     CDS             complement(26078..26635)
                     /gene="frr"
                     /locus_tag="RD477_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDT7633141.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(26638..27360)
                     /locus_tag="RD477_000761"
     CDS             complement(26638..27360)
                     /locus_tag="RD477_000761"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDT7633142.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(27496..28377)
                     /locus_tag="RD477_000762"
     CDS             complement(27496..28377)
                     /locus_tag="RD477_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDT7633143.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGKTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(28476..29258)
                     /gene="rpsB"
                     /locus_tag="RD477_000763"
     CDS             complement(28476..29258)
                     /gene="rpsB"
                     /locus_tag="RD477_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDT7633144.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            29617..30096
                     /locus_tag="RD477_000764"
     CDS             29617..30096
                     /locus_tag="RD477_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDT7633145.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            30313..31404
                     /gene="phnW"
                     /locus_tag="RD477_000765"
     CDS             30313..31404
                     /gene="phnW"
                     /locus_tag="RD477_000765"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDT7633146.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            31397..31525
                     /locus_tag="RD477_000766"
     CDS             31397..31525
                     /locus_tag="RD477_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDT7633147.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            31529..32074
                     /locus_tag="RD477_000767"
     CDS             31529..32074
                     /locus_tag="RD477_000767"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDT7633148.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(32528..34219)
                     /locus_tag="RD477_000768"
     CDS             complement(32528..34219)
                     /locus_tag="RD477_000768"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDT7633149.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(34639..35586)
                     /gene="arcC"
                     /locus_tag="RD477_000769"
     CDS             complement(34639..35586)
                     /gene="arcC"
                     /locus_tag="RD477_000769"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDT7633150.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(35701..36720)
                     /gene="argF"
                     /locus_tag="RD477_000770"
     CDS             complement(35701..36720)
                     /gene="argF"
                     /locus_tag="RD477_000770"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDT7633151.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(36811..38040)
                     /gene="arcA"
                     /locus_tag="RD477_000771"
     CDS             complement(36811..38040)
                     /gene="arcA"
                     /locus_tag="RD477_000771"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDT7633152.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            38501..39202
                     /locus_tag="RD477_000772"
     CDS             38501..39202
                     /locus_tag="RD477_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7633153.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(39811..40827)
                     /gene="tsaD"
                     /locus_tag="RD477_000773"
     CDS             complement(39811..40827)
                     /gene="tsaD"
                     /locus_tag="RD477_000773"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDT7633154.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(40824..41288)
                     /gene="rimI"
                     /locus_tag="RD477_000774"
     CDS             complement(40824..41288)
                     /gene="rimI"
                     /locus_tag="RD477_000774"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDT7633155.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(41295..41837)
                     /gene="rimI"
                     /locus_tag="RD477_000775"
     CDS             complement(41295..41837)
                     /gene="rimI"
                     /locus_tag="RD477_000775"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDT7633156.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(41821..42543)
                     /gene="tsaB"
                     /locus_tag="RD477_000776"
     CDS             complement(41821..42543)
                     /gene="tsaB"
                     /locus_tag="RD477_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDT7633157.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(42734..43714)
                     /locus_tag="RD477_000777"
     CDS             complement(42734..43714)
                     /locus_tag="RD477_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633158.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(43738..45222)
                     /gene="galT"
                     /locus_tag="RD477_000778"
     CDS             complement(43738..45222)
                     /gene="galT"
                     /locus_tag="RD477_000778"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDT7633159.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(45234..46223)
                     /gene="galE"
                     /locus_tag="RD477_000779"
     CDS             complement(45234..46223)
                     /gene="galE"
                     /locus_tag="RD477_000779"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDT7633160.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            46472..46639
                     /locus_tag="RD477_000780"
     CDS             46472..46639
                     /locus_tag="RD477_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDT7633161.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(46701..48512)
                     /locus_tag="RD477_000781"
     CDS             complement(46701..48512)
                     /locus_tag="RD477_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDT7633162.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(48509..48874)
                     /locus_tag="RD477_000782"
     CDS             complement(48509..48874)
                     /locus_tag="RD477_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDT7633163.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(49037..49432)
                     /locus_tag="RD477_000783"
     CDS             complement(49037..49432)
                     /locus_tag="RD477_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7633164.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(49450..50412)
                     /locus_tag="RD477_000784"
     CDS             complement(49450..50412)
                     /locus_tag="RD477_000784"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDT7633165.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(50412..50624)
                     /locus_tag="RD477_000785"
     CDS             complement(50412..50624)
                     /locus_tag="RD477_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDT7633166.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(50645..51343)
                     /locus_tag="RD477_000786"
     CDS             complement(50645..51343)
                     /locus_tag="RD477_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDT7633167.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(51363..51905)
                     /locus_tag="RD477_000787"
     CDS             complement(51363..51905)
                     /locus_tag="RD477_000787"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDT7633168.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(52037..53041)
                     /locus_tag="RD477_000788"
     CDS             complement(52037..53041)
                     /locus_tag="RD477_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDT7633169.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(53038..54027)
                     /locus_tag="RD477_000789"
     CDS             complement(53038..54027)
                     /locus_tag="RD477_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDT7633170.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(54024..54830)
                     /locus_tag="RD477_000790"
     CDS             complement(54024..54830)
                     /locus_tag="RD477_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633171.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            54996..55952
                     /locus_tag="RD477_000791"
     CDS             54996..55952
                     /locus_tag="RD477_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7633172.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(56029..56547)
                     /locus_tag="RD477_000792"
     CDS             complement(56029..56547)
                     /locus_tag="RD477_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7633173.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(56635..56784)
                     /gene="rpmG"
                     /locus_tag="RD477_000793"
     CDS             complement(56635..56784)
                     /gene="rpmG"
                     /locus_tag="RD477_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7633174.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            57012..57458
                     /locus_tag="RD477_000794"
     CDS             57012..57458
                     /locus_tag="RD477_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDT7633175.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(57653..59548)
                     /gene="asnB"
                     /locus_tag="RD477_000795"
     CDS             complement(57653..59548)
                     /gene="asnB"
                     /locus_tag="RD477_000795"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDT7633176.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(59873..60847)
                     /gene="bsh"
                     /locus_tag="RD477_000796"
     CDS             complement(59873..60847)
                     /gene="bsh"
                     /locus_tag="RD477_000796"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDT7633177.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT        17347 a        12911 c        10575 g        20189 t
ORIGIN      
        1 gacaaggttt tacggattca aaaattcagg attatgctat actggtatta aaaaaataat
       61 gaggtatttt atatgaaaaa gttaggtttt attttattgt tggtctcttt attccttgta
      121 ggatgtacgt ctcctagtaa agaaaatagt gatccatctg cgaccacaga caaaagttca
      181 atcaaacaaa ccagttcagc tgaaaaatca agtactcaga aatcagcttc tactgaaacg
      241 aatacatctt caacaagcag taaagtctca gagcccaata cttcttctac atctagtagt
      301 acagttgaga caaatatttc ttcttcaaca atagacacaa cttcctcaca gtcttctcag
      361 tcaatagatc cgttagcagg atattctgat ctacaaattg agtacgcacg tgtttggcta
      421 gcaattagag gtaatacttt tagagataca tcgggtgatt tcgaactaca tgttattcat
      481 tatcctgcag gaacacctat cgccccttat gatgcaggga gtgcagtcta tccgaaagat
      541 gtggttatgc tcactgggaa atacagttac cagggagttt tagtgtattc aagtaaccat
      601 gacgggacaa tcactagtta tccagttcct tcacattggc aaatgcctgc agatcaaaca
      661 tcttatccag cgttcattag agaaacaact caagaaatta ttgataatgc atctgtagta
      721 gctattccta caggtaatcc aaatgatgtg aaacagctga tcaatgtgat agttatggat
      781 aattaaaatt tctaatagat catacaaaaa gaagctggca aaatactgtc agcttctttc
      841 attttctttt ctatttagat ttgttggtct ttgataaact ccaaggcagg aaaaaatttt
      901 ttaaataaac gtttgcaaaa cagtcgcttt tctatcttct accgctactt ctgcataggc
      961 accattgatc aatgtcagtt gtggcggtac atgaccacaa tcaatatcat acaagatcgg
     1021 tagttttaat tcctttgaca gatcttcata tacatccttc attttataat ctaacaaatc
     1081 ctctgctgca cttctaccga acatgatccc cgagctgcca tcaaaccatc ctgccagttt
     1141 catctgaaca agtgatcttc ttaaatcaac tgacgatagc tcacaatttt ctagaaacca
     1201 caagatcggc tttccatcat tatactttct tttaaaagat tggacatctc cataaggggt
     1261 accgattaaa tgactgatcg tatcaataca gccacctaat aatcgtccct tcatccttac
     1321 tgcatctccg ctgactgatt gccaatttgt tggttcagtg agatggaaaa cagaagatga
     1381 tgaatgctca tggggccact ctttttgata cttttcagaa ggttgctgta gaatcgattc
     1441 tccttctctt gttttcagta cactttccca cattgctgtc gtttcatcca agtactctcc
     1501 tcttaaatca ataaggttgg ttccatgtgc agtggctatc cctgttttta atgtgacagc
     1561 aagtagcaac gtactaatat ctgaataacc tactatccat tttggttgta gagcatcaaa
     1621 atcgacaaaa tcgagaatct caatcaataa ttctcctccc caaggcggaa agatcatttg
     1681 gatattttca tcttgcatca tttgattgaa ttcatctgct cgttttcttg ctggacctga
     1741 tttttcctta tcttgcgtcc aaagcgtctt tccagtaact acttggaaag atttttcttc
     1801 cattctcttt aaagctgatt cgagtaacga atggtgttcc tctgatacgc ctgaggatgg
     1861 tgcagtaacc ccaatcgttg catttctttc taataatgga taacttatca tgttgcttca
     1921 cctccaagta tgcactattt tacttggtta attgtttttt tcgtttgccg gcatcaaaaa
     1981 tatcggcatc tcaagtagct ggatgatttt gtttaagtca accgtttgga tgaccatcgt
     2041 ttgatctggt ctacctggtg caaaaacaag tttttcattt gataagagct cttcatcaat
     2101 aataatcgga atctgttttt cataaccaaa cggagaaaca gcgccctgct tttgtcccgt
     2161 ttgttcttcc atgcgttctg atgaaacaat agaaagcctt ttaccgacga tcttccctaa
     2221 tgctttgaaa tccgtttgtt tcgtagtaaa agtcagatat acgtagttat tatctttttt
     2281 atcttttaag aataagcttt tggtctctgt acctgtaaat ccttgttctt tctttattac
     2341 caaagcatct tcatttgtat aaatggctcg atgatgatac aactcaaaat atatattttc
     2401 gtgtaagaat ttctttgcca agtcaatcat ttttatgtct cttttctatt ttattatttg
     2461 agacatctta tcatataaat atagcggtta caacattact tgtgttttgt tcgtttttta
     2521 tacataacta ttgtttttct ggtaaaataa atcaaaccac ttaatggagg cgttttagaa
     2581 tggaaaaatt tcagccgatc atcaatcaaa tcaaagaaaa agaacatcaa caaaagatca
     2641 aagacctttt tgctaagata acagaaacgt atccccagct agacacaact gtaaaatgga
     2701 accaacctat gtttactgac catggtacct ttattattgc atttagtttt gctaaaaatc
     2761 atttttctat cgcaccggaa aaagcagcga ttcgtgccct tgaaaaaaat atccaagaag
     2821 caggctacgt atatacggat aacgtcatca aagttccttg gaaaagtgta atcaactggg
     2881 aattgattga acaattgatt tcatttaata ttgaagataa aaaaggtcat gataaatttt
     2941 ggcgataaag ctttatacga ataaaccttt tactggcgag gaaactattc atcaccttat
     3001 acaggttttg ttcaatccta tcctttttat catccctggt taaacactta attgacaaag
     3061 tatctgctat ttttataagg agtctttctt gtgctgaaaa aaatcactga ttgaatggct
     3121 ttgaattttc tacttagtaa aattattttt tcagcaattt tcctgcaaaa cttttgaata
     3181 aggaaaaaat acgagaaaat atcagtgatc tttcataata ctgcccatta ttttcaggta
     3241 tatttaaata taatatcact ttaggagaga acgaatttct ttagagaaat aaaagttaga
     3301 attttctgac ttgattttta caatgacctt aattaaaatc gttctttcta catttcgtga
     3361 ttaagcaaat atcaactaag tcagtagaac cattcacata ggagggtgaa cattgaaaga
     3421 tttttactca gtaaatgaat tagccgaaca attaggtgtt actacacgtt ctatcagaaa
     3481 ttatcttcat gaaggaaaac taaaaggaac aaaggttggc gggcaatgga aattttctga
     3541 acgtaatctt tttgagttcc tatacggaga tcaagcagag gaagcagcaa aagaaatgca
     3601 gcgattcatg ctaaatgcac caatcacgat gcgtttcaac cttcaatatc gtgattttac
     3661 agcaatcaat caattccgag aacaattagt ccagtaccat aacgatgttt acgcaaataa
     3721 aaaagaccgt cttttacaat acgatcttta taaagacaat catgcggaga tactaatcgg
     3781 tggtaacttc aattatgtcg taaactttag tcaatggatc aatgaaaaat tattgatcca
     3841 gacagatatt tcccttgtat cttaaacaat taaaaagcgc tagaaattcc agagatgttg
     3901 gaaattctag cgctattttt ttataagtac ttactgtttg gttcaaacaa atattttttt
     3961 aataaatgaa attttaccat ctttatcctc aaataaagac cgtgaccatg cttcctacta
     4021 cgatcagcaa taatccgata atcgttaatt ttttttcttt tggtgtctta ttttcatgca
     4081 ggatgtacat accgcctaat gtagagataa ctacgtttag ctgtgtaatg atatatgcag
     4141 tagcaacccc attttcattt gctgaaaaaa tataagccaa tgctgacaca ctaaagatgg
     4201 ctccaacgat cgttgctttc caactcttgt cttcagaaaa tgcggttggt ttacggaaaa
     4261 tcacataaat cactgcccct aaaaagacac cgatcatttg cggaaagaaa atggaaagtc
     4321 cgcttgcatc aactagttta ggcaatgcac tgtataccca ataaccaata cttgttgatg
     4381 ctaatagact aattcctttt gtgataccgc ctttttttcc aggatcatca ctgattgccg
     4441 ttaaagcaga accaaataca agaagaatga ttgctgctgc tccaatgatt ttccccatac
     4501 ttccagacca ttctccaaaa gcgaagacac caatcaacga ggttccgact aactgtaatg
     4561 cagtcgaaat tggcatcgtt ttagaaacac caatcaagtt gaaagatgta tactgtcctg
     4621 tttgtccaat aacccaaaat gcaccagaaa gtaaactaat caaaaatgta gtcactgaaa
     4681 taccagctgg agaagcgaat aattgaacga gtaagccgac aagcaccgct ccaataccag
     4741 ttcctaatat ctcatttgtt ggtctgccac caatcttttt caaaatcagt ggctggatcc
     4801 cccagccaac tgccgggatc aatgcaatca ataaacccat ttttattcac ccttatcctt
     4861 ttccaccttg gaaagaagga tataaagtca tcccaccatc tgcaaatagt gtaatacctg
     4921 taacataact ggattcgtct gaagccaacc atgcagctgt cgccgctatt tcttttggtt
     4981 cacctacgcg tcctagtggg atcatacttt ccaagagttc tttttgttca ggatcatcaa
     5041 attttgccgc attaataggt gtacggatag caccagggcc tatactattg attctgattc
     5101 ccttcggtgc atactccatt gctaccgttt cggtaaataa ttttaccccg ccttttgaag
     5161 cagcgtaatg agcaaacgtt ggccatggga tctgttcatg aacagaagac atattgatga
     5221 tatttccttt tttattattc tctgtaaagt aatttaaagc cgctttcgtt ccgataaaaa
     5281 cgccagtcaa attgactttt aacactcttt cccaatcttc aaggctaagt tcatgggttg
     5341 gatgctgatt ttccatccca gcattattaa cccaaacgtc cagtgtccca aaattttcta
     5401 aagccgtatc aagtaaacgc tgtattcctt cttcagaact gacatctgct tgtacagaga
     5461 ctgcttttcc gcctgcctct tcgattgctt tgactgcttt ttctgctcct tctttatccg
     5521 agttatagtt gacaactaca ttcatttttt ctttcccgaa tcgttgagca atcgctgttc
     5581 caatcccctt tgagccacca gttactactg cgactttgtt ttctaattcc ttgtacatag
     5641 tacccactcc tttttcatac ctaaacctgt tattacggat aacttttatt tccgccttaa
     5701 agtgagtgta caccaagata tatagtctaa agcaaatgat acgctctagc aatcgttttc
     5761 atgaattgct agtgtccttt tatttctata aagaaagagg ttgtgacaga agtggtcagc
     5821 ttcaagaaat ttcggcttat ttccgaagaa gctacttctg aaaaaccatt tatttggaaa
     5881 aaaaggctgt gacaagactt ttgtcacagc ccctttcctg ggcttattta tcacccattt
     5941 tattgtaatt gctagttata cataaacaag agattttatg cataatgttt tattgctgag
     6001 aaaaatattc agctgttgct tgttcatcct ccttgagttg ttcaatcaat tcctctacac
     6061 tagaaaattt cacttgatct cttaaaaatt gattccagcg taccgcgaca tgttcaccat
     6121 agatatcttg attaaagtcc aaaatataca actctacagt cagctcccgt ccttcaccga
     6181 atgtatcatt atgtccaatt gaacccatgg ctggatacca tgtttcccca atttttattt
     6241 ccgacacata aacaccttct ttaggcaaat gaaccgtgct ctttactttg acatttgctg
     6301 tagggaatcc tagtagtctg cctcgtgcat cgccatggac aactacccct tctacctcat
     6361 atgtgcaacc taatagtttt gctgcttctt ttacttgtcc gctgtctagt aatccacgaa
     6421 tacgggtaga gctgattttt tcaccttgtt cttcttcttt agaaactgtg acgattttga
     6481 atcttccttt ggcatattca ggaaaatgag cgacatctgc aatgtctttt ggtccatacg
     6541 tataatcaaa accggaaaca gcatattttg catgtaaccc tacgatgtat tgatcaacaa
     6601 attcctgcgg tgctaaatga gcaaaagaag aagtaaaatc aacttcatat aaataatcta
     6661 tacccaaagc ggccattttt tcttcttttt gcttaaggct agtcaaatat ttcacgctgc
     6721 taggatcgat ctttttaaag acaatagatg gatgctgatt gaaagtcatc aatgctaatt
     6781 tcaatccttc tttttgtgca atttccatac ctgtctttat gactttctgg tgtccgatat
     6841 gcacgccgtc gaaaaatccc agcactaaaa cgacatcgtc ttctggaatt tgttctgatt
     6901 ggtaaggatg tctgatcttg atgatattca ttcgttattc ctcatttctc aatactttac
     6961 taggttttaa tttgtttttt tctttcgggt ttggctgata aatactgaca acttttcctt
     7021 tataaaacaa agcaatttct tctgacggca tttctgggag cgcaaataca gtataatcaa
     7081 gtctcatacc gttttttact ttctgccata cttcatcagt aatatctact cgtttgaacc
     7141 gttccactcc tttttctatt ggcagcaaac aatcatcgat tgtcccattt ttcacagctt
     7201 gagctacttc ttctaatgtc agagcatcct tctgggacat accggcactt gctgttcggg
     7261 tcagatcaga catgtgcgca gggtaaccaa gtttcttgcc tgtatctaca gctaatgtgc
     7321 ggacataggt tcctttcccg cattctactt cgaagcgcca agaaatggtt cctgtcactt
     7381 cgtctcttgt caattcactt gtccggataa aacggtaaat ctgagccttt ctaacaggac
     7441 gctccactgt ttcattattg cgggcatatt catataaccg cttaccgttg acttttaccg
     7501 ctgagtacat tggtggtatc tgggtgatct caccaataaa tgaagccatc acttcatcta
     7561 tttttttttc agataaagac atatctactg gttttgtttc tacccgttct ccggatcgat
     7621 cttccgtcgt tgtactgaaa cctagagtta tctcgcctac atagattttc ccagaatctg
     7681 tcaaatattc gataactttt gtggcttttc caatacaaat gggcaacaca ccgtctacgt
     7741 ctgggtcaag cgtaccccca tgtccgattt ttttcgtatg taagattttt cttaatttga
     7801 atacgcaatc atgacttgtc attccgcgtt ctttccataa aggtaataac ccgtccatgt
     7861 cttttctcca tttcattttt gatttaactg aattattata acacattgca tcaattactg
     7921 acgaatttga catcataatg aacggttttt atttttcggc aataaaaacg taacaaaatt
     7981 catgctcacg taaatagaaa tcatgttatg cttttttaaa ggagggaata gaatggaaga
     8041 ttttttcttt gcaccatttg attggattta tgggtggctt acaggtaaat agtttggttt
     8101 tcacttccca aaaacgaaaa aaaagactgg ccattcttcc agtctttttt ccgttttttt
     8161 tatttatttt ttcagtttct taatcaatgt aaaaaattct attttgcggg atcgatttta
     8221 tcattttatt ggtcaagatt tttccatttc caatccaata cttctggcag atcaatacca
     8281 ttttcacgaa tatagttttg atgttcttgt aatttctgat ccattttttt cgcaaaatct
     8341 tctgcttgac ttcctttgat ttttaaagct gcgtcttttg ccaaatgata acgatcaagt
     8401 tcatttacta cacgcatgtc aaatggtgtc gtgatatctc cattttcttt gtagccatga
     8461 atcattaaat gatggttatg acgatcaaag aaaatcgtgc ggaccaatgt ttcataaccg
     8521 tggaaagcaa ataagatcgg tttgtctttt gtaaaatatt gatcaaattc ttcatcgctc
     8581 aacccacgcg gatcttgact tggatgacgt aatttcaata aatctacgac attgatgaac
     8641 cgaattttta gttttgggaa ttcttccagc agcaaagtta cagctgctaa tgcttctaag
     8701 ttagattccg ttcctgcagc agcaatgacc aattcaggtt cctcctcttt tgctgtcgaa
     8761 gcccaatcaa tgattttcaa gccttcattc actaagacgg ctgcttcttc tgctgagtaa
     8821 aattgagcac gcggatgttt ggaagagatg atcaaattga ttttttctga cgagcggaat
     8881 gctttatcca tgactgcgag caatgtattt gcatccgcag gcagatattc acggatgaac
     8941 tccgccttct tttctgccaa atgtgtcaat attcctggat cttggtggct atatccattg
     9001 tgatcttgct ggaagactgt tgagcttgca atgagattta gcgaaggata atcatttcgc
     9061 caagaaaggt catgtgattt ccgcatccat ttgaagtgct gagtcaacat agagtcaacc
     9121 actcgcaaga atgattcgta acttgcaaaa aaccatgccg tccagtcaga acgtaccctt
     9181 ctaagaaccc ttcatcttgg tgttccgata attgactgtc gatgacgcga ccagatggcg
     9241 aaagccattc atcattttct ggttcgattt tttccatcca ttggcgattt gtcgttttga
     9301 aaacttgatt caatcggttt gattttgttt catctggtcc aaatatacgg aaattttctg
     9361 gatttttctc aatgatctcg cgaacgaatt taccaagttc ggtcatatct tctgctttaa
     9421 cggagcctgg tttttcaaat tgcaaagtat attttttcca atctggcata ataagcggtc
     9481 ttggatcgat tccaccattg gtgataggat tttttgccat tctttggtca ccagtaggcg
     9541 cgatttctct gatttcttct aaaatacgtc cttgtgcatc aaataatttt tctggttcat
     9601 aggatttcag ccaacggagt aaagcatctg catgatccat atgttcttga tctacaggaa
     9661 tcggtacttg gtgtgctcgg aaggttcctt caattggttc cccgtcccat tcttttggac
     9721 cggtccatcc ttttggtgtg cgtacgatca atactggcca ttttggcatc acgacatctg
     9781 ttgctgtctt caggcgagct tctttttgga tggatttaat tttctcgatt gcttgatcca
     9841 ttttttcagc cataacagga tttaattttt ctgaatcatt tccttcaata aagaaaggtt
     9901 cccagccaag tccgttaaag taatttgcta attctccatc tgatttacgt gccaagatcg
     9961 ttgggttggc tatttttgca ccatttaaat gaaggatcgg taaaacagct ccatctatca
    10021 ctggattgat aaagacattt gagaaccagc ttccagctaa aggaccggtt tctgcttcac
    10081 catcaccaat cacgacagca gagattactt caggattgtc taatacagct ccgactgcat
    10141 gtgaaagaga gtatccaagc tctccgcctt catggataga acctggtgtt tctggtgctg
    10201 catgagaagc aatgcctcca ggaaatgaaa atcgtttgaa taatttttgc attcctgtct
    10261 catcttccgt tacttctgga tagatctctg tgtaacttcc atctaaataa gcattcgata
    10321 ccatgacttg tccgccgtgt cctggccctt caatataaaa catgttcaaa tcatatttat
    10381 tgatgactcg gttcaaatgg acataaataa aattttggcc aggaatcgtt ccccaatgac
    10441 cgattggatg ttttttgaca tcttcgggtt tcagtgtacg tctcagtaag gggttatctt
    10501 taagataaag ttgcccaaca gaaagataat tagcagcacg ccaccaagcc gtcattttat
    10561 caaagtattc tttactagaa taatccatac ataacactcc ttttcattat tactcttatt
    10621 ctagcgcaga tactcccaat tgaaacctaa taagcacttc tgttcatttg tttggatttt
    10681 atatctagcc aaatgaacag gtataaaaaa ccacgaaatt tttcctttcg ctgacgtttt
    10741 ttacattgat ccaaaaaact tgtcgttatc acaatggctg ttccgcagtt catttcgctc
    10801 ctcatcatgc ataatttgct gaatgacgct gggccagtta acacattgct ggaaaactgg
    10861 ggctggatcg agcaattcct ttcctgattg atccgtttta tgtaaaagtc tctgttatct
    10921 ttgtcaatat gtgaattggt atcccagcta ctatgctaat ttctacaggg attatccaaa
    10981 atttggatcg tgaccaaatc gaagcggcgt aatcggaaat atcaataact tcaacgtgat
    11041 ttttctttta actgtcaacg gaccgacaaa ctcaaacttt tatggtgcag gttccactga
    11101 tcttctagtt acttggctat acctgactgt cactaaaata gattacaatc tagcttccgt
    11161 tatcggtatc ttgatcttta tctatatgca aaaatttata tactactaga aaaaagaaag
    11221 tcgaattttt tacttttctt tttctctttt ttggttagct gacacgccgc aagactaaag
    11281 tcatggaact cttaggtaat acgtcttaat gaaaacaaaa aaatcccgca acccctgatt
    11341 tcacaggatt tgcgggagtt tttgattagt ctttgttcaa gtcgcgaagc aattcatcga
    11401 tatggttgcc atagacaact gattcatcaa gttcaaagaa caattcaggt gtcttataaa
    11461 tacttagatt gtgccctaac tctcgacgaa tcaatcctga tgctttattc aatccatctt
    11521 gtgctgtttt cttatctgat gctaagtcag ataaaatgct gtaatagatc gttgcttgct
    11581 gtaagtcccc cgtaactttc acatctgtga tcgttacgtt ctccacacga gggtcacgga
    11641 cctttttgcg caaaatatca ttgacttctt ttaagatttc ttgaccgact cggcggtcac
    11701 ggtagttagc cataatgatt ggcctccttt tatcagtttt tagtcatttt tgacttcttc
    11761 catgataaat ccttcaatta cgtcatctac tttgatgtca ttgaattttt caatcattgc
    11821 accacattca taacccattt tgacttcttt cacgtcatct ttgaatcgtt tcagacttgc
    11881 aagttggcct tcatagatta cgattccgtc acggattaca cggacaccgc tatctctacg
    11941 aatatatcct tctgttacat aggcacctgc aatcgtacca actttagaaa ctttgaaggt
    12001 ttcacgaacg atcatttgac cggtgatttt ttcttcaaat tcagggtcta gcatcccttt
    12061 catcgcagtt tcgatttctt cgatggcttt atagatgata cggtgaagac gaatatcgac
    12121 ttcttcagta tcagcttgca cttttgcctg cggtgtagga cgaacgttga aaccgatgat
    12181 aatagcgttg ctggctgctg ctaaggttac atcactttca ttgattgctc cgacagctga
    12241 atggacgatt tttacacgga caccttccac atcaatcttc ttcaatgatg cagccagtgc
    12301 ttctgctgaa ccttgtacat ctgctttgat gattacgtta acttctttca attcgccttc
    12361 tttcaggctt tcaaataagt tatccaaagt cacacggctt gtggcagcac ggttttctaa
    12421 taatgcacgt ttcgcgcgtt cttcacccgc tgcacgtgct gtcttttcgt cttcgaagac
    12481 tacgaaacgg tcacctgctt gaggtacatc attcaagcca gtgatttcta ctggcgttgc
    12541 aggtcctgct tctttatcgc gacggccaag atcgtttgtc attacacgga cacgtccgta
    12601 agtattaccg acaacgattg ggtcaccaac atgaagtgtt ccttcttgga caagtaaggt
    12661 tgacacagga cctttacctt tatcaagacg tgcttcgatc actgttccga ttgcgcgttg
    12721 tgttggatct gcttttaaat cttctacttc tgcgactaac aggatcattt ccagcaattc
    12781 ttcgatgttt tggccgaatt tcgctgagat ctcaacaaag atcgtttctc caccccatgc
    12841 ttcaggaatt aactcatact cacttaattc ttgcataaca tgttgagggt ttgcacctgg
    12901 tttatcaatt ttatttacag cgacgatgat tggaacgcct gcagctttgg catggttgat
    12961 tgcttcaact gtttgtggca tgacgccatc atctgcagca acaactaaga tcgtgatatc
    13021 tgtgatactt gctccacgtg cacgcatact tgtaaaggcc gcatgtcctg gtgtatccaa
    13081 gaaagtgatt ggtttaccat caatatcgat ctggtaggca ccgatatgct gtgtgattcc
    13141 gccggcttcg ccgcttgtta cacgtgaatg gcgtaatgta tccaataaag ttgttttccc
    13201 atggtctacg tgtcccataa tcgttacgac tggcggacgt gagactaatt tatctggatc
    13261 gacttcttct gcctcgaaga atttatcgat atcagcaatg tcaacttgga ctttttcttg
    13321 tggttccatg ccatagtccg tcgctaacag ttcaatcgta tctttatcca gtgcttggtt
    13381 ttgattgacc atgacaccaa gcataaataa ttttttaatg atttctgctg gctcacgatg
    13441 aatttttttc gcaatatctg ctacgttcat accttcagta tactctaata catctggcaa
    13501 ttcacggaat ttgcgtggtg gtacagctgg tttagttgat tgttgctgtt tgcctttttt
    13561 acctttttta ttataacgat tacggttatt attattaaat ttgccgcgat tgttattgtt
    13621 tggtttgttg ttattgttag tacggttttg attgccgcct tggctcgttc cttgacgatt
    13681 ttggctgctg gtatttcccg tattattttg gtttgttgtt ggtttttttc cttgattcac
    13741 ttgaccgctc ccttgaccgc gatcttgata actgcggtta tttttatttt tttgttgatt
    13801 gttcgttgtt tcttttttac gttgatcgtt cggctgttga ttggctggtt ttctgcttgc
    13861 ttgttgtgca gcttgttgtt tcgtgcttgg gtttttgaac gcttgttgca atttcttctc
    13921 atcattgcta gtaatcgcac ccatatggtt tttcacatcg atgccaagtt gttgtgcttt
    13981 ttccacgacg tctttactag attttttgag ttcttttgct aattcataga ttcttttgtt
    14041 gcccatgcaa tcaccttcct aaccttcaat tagctccttg gtcttcttcg caaatccggc
    14101 atccattatt ccgaatacct tgcgaggttt tccgatcatc tgcgtgattt ctgcttcaga
    14161 aaatagctca aagcaaggaa cttcataata tgaactctta tcttttatct tttttcttgt
    14221 attctcgctt gcatcggttg ccacaaacac caattttgct ttttgtttgc ggatttcagc
    14281 aatggtcagt tcttcacccg tgattagttt tcccgcgcgc atcgctagac cgatgagatt
    14341 catggctttt tgccgattaa ctgtattcat tttccaaata actctttacg tgctttttgg
    14401 tgagtgacat agtcaagaag ttcttgataa aactcatctg ttaatgttgt ttcgagtact
    14461 cgatcgagaa tttttttatc ccaagcttgt tgtacttctt ccggctcaag tgagacatac
    14521 gctcctcgtc caggcatttt tccagttgga tcgatcgata cttccccttc ttttgaacgg
    14581 gtgattcgga ccatctcttt tttaggtttc atttcacctg aaaccacgga tttgcgtaaa
    14641 ggaattttcc tttgtttcat ctctttgcct ccttactaaa gttaagcttc tggtttttct
    14701 tcgaccgttt cattgttgaa tgcaattgtt tcatattcat cgtctgttaa atctgactga
    14761 acgattgctt catcatgcag ttcttctgtt tctttttgtt gttcttgatt ttcataaaat
    14821 tccgtcatgt cagattctga tttgatatcg attttatggt tagtcaattt agcagctaaa
    14881 cgtgcgtttt gtccgcgttt accgattgct agcgataatt ggtagtctgg aacgacaact
    14941 gtgcaggcct ttggatttgt ctcatcgaag atcacatcca tgacttgtgc aggattcaat
    15001 gcatttgcaa tgaatacagc tggatcttca ttccattcga cgatatccat gttttcgcct
    15061 tttaattcat tgacgattgc ttgaactcgt tgtccctttg gcccgacaca tgtaccgact
    15121 gcatcgatat ttggatcatt tgagcgaact gccacttttg aacgatcgcc agcttcacgg
    15181 gcgatgctga caatttcaac gattccatca tagacttctg gtacttcttg ctcaaataag
    15241 cggcgcaata ggtctggatg gctgcggcta acaaatactt gcggaccttt agacgtattt
    15301 tctactcggg aaacataaac tttgattcga tcatgtggtt gataaaattc atttggcatt
    15361 tgatcttgtt tagaaagaac tgcctcaatt ttaccaagat tgacataaat ataacgttta
    15421 tcctggcgtt cgacgattcc ttgcatgatg tctttttcat aagcactgaa ttcgttataa
    15481 ataatcgttc tttctgcttc acgtacacgc tgtaaaataa cttgtttagc cgtttgcgca
    15541 gcgatccgcc caaaatcttt tggtgttact tcaaaacgga tcgtatcacc gatctcatat
    15601 gctggattga ttaaaagtgc atctttcaat gagacttcca actgtgaatc catcacttct
    15661 tccgttactt cttttaccgc atagacatgg atctttcctt ttttctgatc aaattccact
    15721 tcgacgtttt gtgcttggcc atagtgacgt ttgtatgcag aaaccaatgc tgcttctaaa
    15781 gcatcgatca cgatttcttt tgagatccct ttttcagctt ctaaagcgtc taacgcattt
    15841 aacatttctt tactcatttt ttctgattcc tctccgtgat taaaattgga tagctaagcg
    15901 agcttttgca atattttttc ggtcaaaagt caattctttt tctcgtgttt ttatgcgtat
    15961 tttcaatgta agctgttcag catcaaatga ttgaagaaat ccttcatatt gtttttcgcc
    16021 atctactggt tggtataagg agacatggac ataatcacca agtgcctttt catagtcgct
    16081 ttcttttttc aatggccgct ctgctcccgg ggaggatacc tccaggaaat aagcttgagg
    16141 aatcggatct ggatctgctg catcaagttt ttcactcagt ttctcactga caaaggcgca
    16201 ttcttcgata tcgataccgc cttctttgtc aataaatact cgtaagaacc agctttttcc
    16261 ttcttttaca aattcgactt cgacaagttc aaaattttgt tcatctaaaa tcggtgtcac
    16321 catttctgtg actgtttcaa cgacgctact caaaagtttc gcctccttaa aaaattaagt
    16381 gcgtaattcc attaaaaaga gtgagcgaaa aatccgctca ctcacagtta gtatcattac
    16441 ctataagaac tataccatac tctgcttaaa attacaagca gacaggaaaa cagatgctta
    16501 tagcatatcg aataacgata attgattttc atctggtaga tctttcaata cgccattgtc
    16561 gttcatatat tcgatcaatg tttttgaaac tttaccgcgg gtggcaagat cttctttgga
    16621 taaaaatgga ccgtcttttc ttgcttcaac gatttgtttt gccacgttgg ttcccaaact
    16681 tgggacagct ctaaatggtg cgatcaacgt atcaccttca ataacaaagt taacggcatc
    16741 cgatttatac aaatcaatca ttccaaattt gaatcctcgt tccagcattt cattggctaa
    16801 ctccagcact gtcagttgat tcttttcttt tacggaagca tctaatcctt tgtccgtgat
    16861 ttctttcatt gcttgtttca cagcatcttt tcctttgcac atggagacta gatcaaagtc
    16921 gtctgcacgt acagaaaagt atgcacagta atacaaaata gggaaataaa ccttgaaata
    16981 agcgacacga agagccatca atacgtaagc tgcagcatgg gctttcggaa acatatattt
    17041 gatttttgaa caagaatcga tataccagtc gggcacatta ttttctttca tcgccgatag
    17101 ataggtttct cgcaattcat cagggatctt gttccattgt cctttacgaa cagtttccat
    17161 gattttaaat gccatcccgc tatctaatcc agcatggatc aaataaacca taatatcatc
    17221 acgacagccg attacttccg ccagtgtcgc atctcctcgt cgaatcaatt cttccgcatt
    17281 tcctaaccat acgtctgtac catgagatag tccggaaatt tgcagtagtt ctgcaaaagt
    17341 tgtaggatgc gtttcttcta gcatccctcg aacaaaacga gtgccgaatt ctggaatacc
    17401 taatgtccct gttttggaat aaatttgttc ctgacttact ccaagtactt caggaccggc
    17461 aaatatccgc atcacttccg ggtcatccgt agggatcgtt tgtggatcga tccctgataa
    17521 atcttgaagc atccgaataa cagttgggtc atcatgccct agaatatcta atttcaatac
    17581 attatcgtga atcgagtgaa aatcaaaatg agtcgttttc cattccgaat tttgatcgtc
    17641 tgctggatat tggatcggcg taaaatcata gacatccata taatcgggaa taacgatgat
    17701 cccccctgga tgctggccgg ttgtccgttt gactccagta gcgccttttg ctaatctgtc
    17761 gacttctgca cttcgaaact gcagattatt gtccctttcg tatcctttta catagccata
    17821 tgctgttttg tcggcgactg taccaatcgt tccggcacgg taaacatagt cttcgccaaa
    17881 caatactttc gtatagttat gggcttctgc ttgataatca cctgagaagt tcaaatcaat
    17941 atcgggcact ttgtctccat ggaatcctaa aaatgtctca aatggaatat catgaccgtc
    18001 tttgtttaag cgtgcaccac attttggaca ttgtttttct ggcatgtcaa atccagaacc
    18061 atacgtacca tcttcaaaaa actcggaata ttggcactct ggacaataat aatgaggggc
    18121 taacggatta acttcagtga ttcctgtcat cgtcgcaaca aagctagagc caacagagcc
    18181 acgtgaacct actagatagc catcttcatt acttttatgc actagttttt gggcgatcag
    18241 ataaataacc gagaagccat taccattaat agaattcaac tcttttttta atcgtttttg
    18301 aatgatctca ggtaacggat ctccgtacat ttgttttgct tttgtgtaac tcaattctga
    18361 tatttcatct tctgaacctg gaatcttcgg tgtataaagt tcatctttga ctggaatcac
    18421 ttcttcgcag atatctgcga ttttattcgt attttctact acgatctctt tcgctgtttc
    18481 ttcccctaaa aagtgaaatg cagttagcat ctcatcggtt gttcggaaat ggacatccgg
    18541 caaactatga cgattcaacg gattcgctcc gcccatagaa ttgatcaaaa tttttcggta
    18601 aatggcatct tcttcgttca gataatgtac attacctgtt gcaacaacga ttttatccaa
    18661 actttttccg atttctacca aattttggat aatttcttcc aaatcatgtt cattttttac
    18721 caattcttgc tcgatcaaag gcgcatagac cgcttttggc ataacttcaa tataatcgta
    18781 gaattttgct cggtttcttg cctcttctac ccctttttgc atcatggctt cgaatatttc
    18841 tcccttatca caagctgagc cgatcaatag attttcacgc atctttttca attgggagcg
    18901 tggtattcgt gggacgcgtt cgaagtattc gacattcgac attgaaatca gtttgaacaa
    18961 atctttcagt cctgcctgat tttttgctaa aatcgttaca tggaaaggtc gagccctttt
    19021 gtaagaatct ccttctccga tatgctcatt tagctgatca tgataaagca tgttatgatt
    19081 gtccattgcc tctttcacaa agatccatgc taagtgacca gttgcttcag catcatagat
    19141 tgctcggtga tgctgttcca agctgacacc aaatttttta gataacacac ctaatccgaa
    19201 acgtttgaat tgaggataca gatatctagc taattccaac gtatcaatga ctggattggc
    19261 cgcttcagga ataccatacc ttgcgtagct agtattcaag aaccccatat caaaagccgc
    19321 gttgtgggct accaaaattg tatcttttga aaattcaagg aacatccgca atacttcttc
    19381 ttccgacttt gatcctcgaa ccattccatc cgtgatacca gtcagatcta ccgttgttcg
    19441 tgacaaagga tgtcctggat cgataaattc atcaaagctt tcgatcacat ttcctttata
    19501 cattttgact gcggctagct cgatgatcgt gtcatatact gcagacaatc ctgtcgtttc
    19561 cacgtcaaat acgacatatg tggcttcact caatgcttca tgtgcatcat tataagcaat
    19621 tgggacacca tcatctacga cattcgcttc cacgccgtat aagattttca cacctgcttt
    19681 ttttccagca ctatgtgctt ccggaaaggc ttgcgcacca ccgtggtctg taatggcgat
    19741 agctcggtgt ccccatttcc ctgcttgtgc cacaagatca gagatgctgt ttgttgcatc
    19801 catcgtactc atattgctat gaacatgaag ctctactcgt ttttcccctt caggagcata
    19861 atctttgcga ggtgtatgtt tgacttctat gatatcttgc gcattcatga ccaaatcacg
    19921 gacaaacgta tcttcttgaa tacttcctcg tacctttaac cagctaccga cactgatcgc
    19981 atcaaatact tgttcatctt tttcgccgtt cgagaatttt ttgacgataa atgatgaagt
    20041 ataatctgtg attttcaaag tcaagatctt gcgttttgaa cgtagttctc tgacttcttt
    20101 gtcgaatacg tacccctcaa gtgtgactct tcgttcttct tcaacgatac tgatcattgg
    20161 tgtcgttggt tcatcggctg gaatatttcg tcctagctgg atgtggatgg gtccttcaag
    20221 agctggcgat tgctcttttc tttccttttt cttttgttcg tggacaacta gactttcagc
    20281 tgcttgcttc ataaaggctt ctgcctgttc ttgtttgcgt tcttcaaaaa gtttcaatac
    20341 tcgttccgct tgctgctcat ccacttctgg ttcgatacga aattttggga atccgtaact
    20401 gacgaacaac tcttctacta atggcaagta ttgttgcttc aaataagaaa tagctcctgt
    20461 actgtcgatt ggcaagatga ctttacgatc ttttatcaca ggtacctgcg ttttcaatac
    20521 ttgctgtgct agtggtgtat cgcattgctg attttccaaa gcttgggccc agtaatcttg
    20581 caagagctgt tcatcgaatg tctggtcatc tgctaagata ttcagtttga cttgtgcaat
    20641 atctttaaaa gcgatagtca gatgctgagt caatgaccga tatagcataa taggcaaaat
    20701 tttggaaaaa tgcagcgtaa attcccataa acgcgattta cgatggacca ctacactatc
    20761 catttcacca gcagtaagca gtggatgatt tttttctgct tcctctagtt ggatttgttc
    20821 caacagtttt tcaaataaat ctcgtttttc tgacaaagat atacgccctt tctgatgaga
    20881 aacaatgcaa ttgatcgcat cttatcctct tctttttcag taccttttag tatactcgtt
    20941 ttttctgagt tttacattaa aaaattggaa ctgcgattca aatcgcagtt ccaattttaa
    21001 aagcgaatca tcagattgtc aaaaacagct gattcagcta tttttatttt ctgtgtttta
    21061 acgcttatta ttcgccttgg ttcaagagga ttggtaaagt attcaccaat tcttctttac
    21121 gtacttcaac catttcgcca gttttcttga ttttcacttc tacgattcca tccactgctt
    21181 ttttaccgac agtgattctg attggacagc caatcaaatc agaatccgcg aatttcactc
    21241 ctgcgcgttc atttctgtca tcaaccagta cttcatagcc agcttttgtc atcgcttctt
    21301 ctagttcatt cgtcaggttt gtttggtatt cgtcttttag attcatttgg acgatatgca
    21361 aatcaaatgg cgcaattcct tttggccagt tgattccttg ttcatctgaa tgttgttcta
    21421 cgattgctga taataaacga cttacaccga taccataaca gcccataatc acaaattttt
    21481 ctcgtccatt ttcgtctagt acagttgctc ccatagattc actgtaacga gtgccgattt
    21541 tgaagatatg tccgatttcg attcctcgag taaaggcaag aacaccattg ttgtctggtg
    21601 aaggatctcc ttcttgtaca aaacgaagat cttcataaga aagtacagtg aaatcgcgtt
    21661 ctggattcac atttttatag tggaatcctg tttcgttcgc tccagtaatg gcattcgcta
    21721 aatcttggac ataacggtct gcatatactt ttacgtcatc tgaaacaccg actggtccga
    21781 cagaaccaaa ctcagcacct agatattttt gtgcttcttc ttctgttgct tcttctaaaa
    21841 agtcagcaga taaaaagttt ttcagtttta cgtcattgac ttcatggtct ccgcgtacca
    21901 aaacaaggac aggttgttca tccgcaatga acaatacaga cttgatgatt ttttgaggtt
    21961 caacagaaaa gaaatctgct acttcgtcaa ttgattttac gttaggtgta gctactttct
    22021 ccaattcaag ctgtgtttca tgcgattttt ttgatacata gtagcttgta gccatctcca
    22081 agtttgcagc ataatcactt tcagtcgaat aacagatcgt atcctccccg atttctgaga
    22141 tcgccatgaa ttcttttgag tctttcccgc ccattgcgcc accgtctccg ataatcgagc
    22201 gaaattctaa gccacaacgc ttgaatactt cggtgtaggc tttttcgtaa tctttatatg
    22261 tctcgtccaa actttcttcc gttgcatgga aagaatacgc atctttcatg ataaactctc
    22321 ttccgcgaag caaaccgaag cgaggtcgtt tttcgtcacg atatttaggt tggatttgat
    22381 aaagatttaa aggtaatttc ttatatgaat tgatttcatt acgcatcaaa tcagtaaaag
    22441 tttcttcgtg agttggtcca aggatcattt ttcgatcatt gcggtctttg aaacgataca
    22501 agtttggtcc atatgtttcg taacgcccgg attcttccca aagttctgct ggcaagatgg
    22561 caggcatcag catttctacc gctccgattt tttcaaattc ttcccgcata atgcgtttca
    22621 gtttttccaa tacacgattt gctaatggca gatatgaata aatacccgct gatacttggc
    22681 ggatatatcc agctcttagc aagatttgat ggcttaatac ttctgcatca tttggaactt
    22741 cgcgtaatgt tggaatcaac atttttgatt gtctcattct atatactcct ttatttcctc
    22801 aaaatagtta tcgtttgtta ttatacccta tttgtcaact ttttctagaa aaagaatcgt
    22861 tgaatatcgt tccatgtaac tagcaccatc aacaacatta aaaatccaaa gccagctaat
    22921 gtcaatattc cttctttttc ttgacttaat ggtttcccgc gaatcccttc aaaaatattc
    22981 aagactaatt tcccgccatc taaggcagga atcggaagca gattgacgat tccgagattc
    23041 attgaaagaa gcgccatcaa actaataaca gttgttacac cttgatttgc tgcttctgaa
    23101 gacaattggt acatcatgac aggtccgcct aatttatcta aactgaaacc tgtaaatagt
    23161 gatcctaagg ctttgaaaat ttccaatgaa ccgctgaaag cttgacgcgt ccctccaatg
    23221 atcttatcca taaaacctgt attcattggg gcttttatac caagttggcc aactttttcg
    23281 ccatttgact ctacagattt cggtgtcacg tcgacagatt gcacctgacc ttctcgttcg
    23341 atcttaaagt ctaatgtttt atccggattt ttcgtaatga ctgttgtcag atcattccat
    23401 gaatggattt cttttccgtc cacactgact acctcatcat tttcttttaa gcctgcttct
    23461 gccgcagctc cattaggcat gatctctcct acgcgattag tgttcgtcac ttgaacacca
    23521 ccttgcataa atgccagaat gatgaataac aggattgcca aaataaagtt attcatgggt
    23581 ccagcaaagt tggtcagcat acgctgccac aatttagccg attggaactg tacgtctttt
    23641 ggcgcaattc tgatttctgt tccgtcgttt tcaataatcg tcgcatcgtg atcaacaggg
    23701 taggttacta cttctgtttc gtctccattc acataaccag tgatagtcag ctcatcttct
    23761 aaatcataac ggctcaattc catcgggata gcatttgtca attggatttt cttacttaaa
    23821 tttatcttct ctacgatgcc gtcagaattt agtaaaagag aaagaggcat tcctggtgcc
    23881 atttctgttt catcatcccc attgcctgcc attcgaacat aaccgccaat aggtaatagg
    23941 cgaagcgtat atgttgtccc gtcttttgct tgatgcccat aaattttcgg acccataccg
    24001 atagcaaatt cgcgaacgag gattcctgat cgtttcgcaa agaagaaatg accaaactca
    24061 tgaacaatca ctaatatacc aaaaacgatg ataaatgtca gaatcgtttt catagctttt
    24121 ttctccttta cttgtttttt gtgtttttta taaaacaacg ataaaacact ataattttac
    24181 catagtatac caaagacacc aatagtatca atataatgaa aaataaaaac ctgcgtcttt
    24241 tatgaaattc acaaagaaac gcaggtatac ttactaaata atgccaaata aatgcatgat
    24301 aggaaataca aacagcaaac tgtcgaaacg gtctaagatt ccaccatggc ctggtaagat
    24361 atttcccgag tctttcacat cataatgacg tttgattgca gattcgacca aatcaccaaa
    24421 ttgtccgact gctgaaaaaa taatcgtcat cagaagcatg acaaacatac catgattaaa
    24481 tagttctttt gctgggtata gcaataaata aagaaatgcg actacaacag cactaccaat
    24541 accaccgagc gcaccttcaa tcgttttgtt gggcgaaata tcaggactta acttatgctg
    24601 accataacgt cgaccaacca tatatgctcc gatatcagtt gcccaaacaa taaatagacc
    24661 aaatatcaaa acagccaagc tttctgttcg tgcattgaca aaattctgga aacctattcc
    24721 cacatacaag ctgacaagca caggaaaacc tgcttcatca atcgtatacg tatttttaga
    24781 aataactgat gttcctaaaa gaatcataac tataagataa aataacataa atgtacttac
    24841 attctctgga agaaagaaaa accatctttc ttttggcaaa acaagtaaca ctgcaccaac
    24901 ggcggataaa actccttcaa aacttaagag tgtcaatcct ttcatccgaa acagctcgta
    24961 aactcctatt actgctaaga ttgctgctgt aagctcaacc gctatgcctc cgatccatat
    25021 aatcggaata aacaagacta gcgccacggc tgctgtgacc acacgttgtt tcatgactga
    25081 tcccctttct catcttctgt tttttttaca ccgccgaatc gacggtctcg attttgataa
    25141 gaggcgattg cttcttcaag atgcgcacca tcaaaatcag gccaaagcgc ttttgtaaaa
    25201 tacagctcac tatatgcgat ctgccataac agaaaattac taattcgctc ttctccactt
    25261 gtacggatca tcagctcagg atcttgtaac tctttaggca agaaacctgt cattaaatgc
    25321 ttagcaatca attcatcatc gatttcttct gcgtgtattt ctccttttgc tacttcttcg
    25381 gcaatttctt ggacagcagt cacgatctct gctctgcttc cataattcaa agcaaaattc
    25441 aataccattc cagtattgtt ttttgtttgc tcaattgcac gtcttacagc atcttgcgta
    25501 tgttctggca atacgttttc ataacccatg acatgaactt tgacattttc tttaatcaat
    25561 tctggtacga acgtatcaaa gaagtcgaca ggtagttgca tgagaaaact tacttcatct
    25621 tttggtcgct tccagttttc agtcgaaaag gcataaagcg tcaacacttt cactccaagc
    25681 cttgatgctt tttttgtgac tttctttact gtttccatgc cttctttatg gccagcaact
    25741 cgtggcaaac gacggttttg cgcccaacga ccattgccgt ccatgatgat ggcaatgtgc
    25801 tgaggaattg gtccatcggt attaaattga aattcgcttt tttcttgaat atatttgttt
    25861 ttttgcggaa aaaaacgtaa cattcttttt cctcctatac ttaaaagatt cccactatct
    25921 taagtattat agcaatatct aactgttttt cctatgaaat cattgttttt ttatttaaaa
    25981 actgaataat tttttctttt tttaattttt aaataaaaga aatataaata aaaacagagg
    26041 aagcggaatg atccgcccct ctgtttttat gattttatta aacttccaat aattcttttt
    26101 ctttatcagc agcgatctta tctacttctt tgatgctgtt atctgtcaat gtttgtacgt
    26161 ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc atttttttgt tgttttttat
    26221 attcatcgat cgcatcacga cggatgttac gaatcgcgat ttttgcattt tctgcttctt
    26281 ttttaacatc tttagctaat tcttttctgc gttcttctgt cagttgcggg ataactaagc
    26341 gaatgacatt tccatcattg gttggactga ttccgatatc acttgccata atagcttttt
    26401 caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat taaaacacgt gcttctggaa
    26461 tttggattga tgccatttgg ttcaatggtg ttggcgcacc gtagtagttc acagtaatac
    26521 ggtccaaaag gctagcgttc gcacggcctg cgcggatttg cccaagttca cgttgaagat
    26581 tttgtgctgc ttttttcatt ttttcttttg cttctgtcat aattgcatct gccattttta
    26641 tttccccctt acagttgttc cgatattttc gcctaagatt gcacggcgaa tatttccgtg
    26701 ttcatttaaa ttgaagacaa gcagtggaat atcattatcc atgcttagtg aacttgcagt
    26761 tgaatccatg acttgtaatc ctttggcaat aacttccaga tgagtcagtt cttcaaattt
    26821 tactgcattt gcatctactt tcggatcagc agaataaaca ccgtctacat tgtttttagc
    26881 catcaggata acatctgcgc cgatttctgc cgcgcgtaat gctgctgttg tgtctgtcga
    26941 gaagtacggg ttgccggtac cgcctgcaaa gataacgatc cgtccttttt ctaaatgacg
    27001 ttctgcttta cggcgaatat atggctctgc gatttgacgc atttcaatcg aagtttgcac
    27061 gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa gctaaggcat tcataactgt
    27121 agctaacata cccatgtaat cagcttgagc acgttccatg cccatttgtg ctccgatttg
    27181 tccgcgccag atatttccgc cgcccacaac gattgccatt tcaacgccca gctcgtgaac
    27241 ttctttgatt tcttggatga tttctttgat tactggtggt ttgattccaa atcctgcgtc
    27301 cccagctaaa gcttctccac tcaattttaa gactacacgc tgatatttcg gtgtgaccat
    27361 gttcattcct ccaatatttt ctattgccat tttaccatac ttagatgatt tcgacactct
    27421 aagactggtt tttcttgttt tgcttaaaaa aagggagcgc acaaaccgtg cgttcccgtc
    27481 tgttattcaa cagaattatt ttttcacttg gctcataact tcttctacaa agttatcttc
    27541 gcgtttttcg ataccttcgc caacttcgaa acgtacgaat gttttaacag ttgcgccttt
    27601 tgatgcaacg tatttttcaa cagtcatatc aggatcttta acgaatggtt ggtccaccaa
    27661 agcgatttct gctttgaatt tcttcaagcg tccttctacc attttttcaa cgatattcgc
    27721 tggtttgcct tcgttcaatg cttgttctgt aagaacagtt ttttcatgtt ctaattcagc
    27781 ttctggaatt tgtgattcgt ttacataacg aggattgata gctgctacgt gcatagctac
    27841 gtctcttgca actgtttcgt cagtttttcc atctagaaca gtcaatacgg caatacgtcc
    27901 gcccatatgc aagtatccac caaatgcagc attgtcgtct ttttctacaa cttcaaagcg
    27961 acggaagctg attttttctc cgataacttg agttgcttcg atcaagtcag attcaattgt
    28021 acctttatct gttttgattt tcattgcagc ttccatgtct gctggtttgt tttcagcaac
    28081 aagttctgca atttctttta ctaaatcttg gaacatttcg tttttagaaa caaagtctgt
    28141 ttctgagttt acttcaacga ttgctgcagt gttacctttc acagcaactg aagccaatcc
    28201 ttcagctgca atgcggtcat ttttcttcgc agcctttgcc attccttttt cacgtaaaag
    28261 atcaactgct ttttccatgt cgccttctac ttcaactaat gcttttttcg catccatcat
    28321 tcctacgcca gtcatttcgc gtagttcttt taccatttta gctgtaacgt ctgccatgtt
    28381 taactcctcc tgtgtgtttt tctttttaaa aaaagctgct tcaaaggaga gtgaggcttg
    28441 tcgcccagcc tttgaaacag ctccatcatt caaaattatt ctgctgaaga attgtcgcct
    28501 tcaacaacat ctacgatttc ttcgattgat gttgctgtgt tttcttctgt gaaatcttct
    28561 tcaactactt gatcttcacc ttggtttcct tcgatgaaag catcagccat ttttgaagta
    28621 atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga ttactacgtc gatttcatct
    28681 ggatcgcagt ttgtatcaac catagctacg attgggatat ttaatttgtg tgcttcttga
    28741 acagcaatac gttctttacg tgggtcaacg atgtacatta catctgggat tcttggcata
    28801 tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt ctttgttcaa accagcaact
    28861 tcttttttag gtagaacttc aaaagttcca tcttcttcca ttgcattgat ttgtttcaaa
    28921 cggctgatac gtttttggat agtatcccag tttgtcaatg ttccgcctaa ccaacgatgg
    28981 ttaacgaagt attgtcctgc acgagttgct tcttctttga ttgcttcttg tgcttgtttt
    29041 tttgtaccta cgaataatgc tacgccgcct tcttcagcaa cgctcttcat gtaatcataa
    29101 gctgcatcta ctaatttaac tgttttttgc aagtcaatga tgtagattcc gtttctttct
    29161 gtgaagatat atttcttcat ttttggattc cagcgacgag tttggtgacc aaagtgtacg
    29221 ccggcttcta gtaattgttt catagaaatt actgccatgt ttgtttcctc cgatttggtt
    29281 ttatttttcc tcttcttatc ttctaccttg ccggcaaact ttttcaagca cctaccgaca
    29341 atccaataag aatgtggatt tgctgatgca taaagcaccc tcagctatta tacagacaaa
    29401 agatccgtat cgcaagagca aatatacttt tctgctcaac attcaatagt ttacctgaaa
    29461 gataaaaaga attcaagaaa aacagtagat ttctctttgt ttctttatta tcctttttcc
    29521 ataaaataat tttaggtata aaagacgaat attattgggc aatccttctt ttttttgtta
    29581 tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc gcaaagctca aagacatttg
    29641 ctgattaaac aagtcattga agaattcccg atccgtacgc aagaagaact attaaacaaa
    29701 cttgggcatt atggcgttac cgccacgcag gcaacgattt ctcgagatat ccgtgatcta
    29761 aaaatcgtga aggcacctga tgaaaatggc gtttcccgct tcgtattgtt tcgcgaaaaa
    29821 ggaactcctg agactaggac tgaggaagag gatcggctgg ttcaaatgat tgaagacatt
    29881 gtattgaaag tcgaacgagt ccactttttg acgatcgtca ataccttgcc agacaatgct
    29941 catttatttg catctgtttt agatgagatc aaacctccct ctattgtcag tacaattgct
    30001 ggattcgata cgatcatggt catctcggaa aatgaggagg acgcaaaacg agtagaagaa
    30061 tttcttcacc atccaactga agtatcactt ttttaatcag agcacgtatt cctaatatgg
    30121 aatgcgtgtt tttttatttt caatattttc cttattgaat aaacctttac actctttaca
    30181 aaaatacatc attttttatt caaatttttc aacaccttga ttgaatcaca ataatcatca
    30241 tttatattgg atgttaacaa gagcagcttc ttgctctttg tgtatttcaa aaaagaaatg
    30301 aaaggaaatc atatgtataa attattaact tctggaccat tgacgacttc tgaaacagtg
    30361 aaaaatgcta tgctgatcga tcattgtact tgggatcaag aatacaaaga aattacacaa
    30421 tggataagaa aaaaattatt ggatcttgct caagtatcgg aaggaatata cacaacggtc
    30481 ttaatgcaag gtagcagctc cttcggggta gaatcggtat taagttctgt gatcaggcca
    30541 gaagatactg tactgatttg ttcaaacggc gcatatagac aacgaatgat caacatgtgc
    30601 gaatgtctga aactgaacta taccgtttat gaggtatcgg aacatcaaat cctccaagct
    30661 catgtgatta cacagctgat tgaagagaat ccgtctatct ccgcactagc aatggtccat
    30721 agcgagacta cttccggtat tttgaataat ttggaagaga ttgctgctgt cacacaagaa
    30781 agagatcttt tgtttatagt ggacgcgatg tcaagctttg gtgggatacc gatttgtgta
    30841 gccgaactag ggattgattt tttgatttcc agctcaaaca aatgtattca aggtgttcca
    30901 ggcttttctt ttgtacttgc taataaagcg gaactggaaa aacgaaaaaa tgttagccga
    30961 agtttgtcac ttgatctata tgaccagtac cgcgtgatgg aaaacgagaa tggcaaatgg
    31021 cgtttcactt cccctaccca tacggttttg gcttttcaac aagcgctgaa ggaactagag
    31081 gcagaaggag gaatcgaaac tcgatattca cgttatctcg ccaataatca tgtgctacga
    31141 caaaaaatga cagagatggg gttccagcct ttcattcaag aatatcaggg gccattcatc
    31201 acctcttttc tttaccctaa ggactggcga tttgatttta atcattttta tcagtattta
    31261 aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg atcatgaatg ttttcgtatc
    31321 ggaaatattg gcgagatcta tccggaagat attttggaac tgacagcaat tatcaaagac
    31381 tataaggaga acgttaatga ttaaaactgt aatttttgat tgggcaggaa caactgtgga
    31441 ctttggctgt atggctcctg tccacgcttt tagaaatgct tttctagaaa aaggaatcca
    31501 actgactgac aaagaaatcc gctaaccgat gggaaaatta aaatgggatc atatccaaca
    31561 gcttctttcc ctcccttctg tcaaaaaaca atggatagct atttatggac aactacctca
    31621 ggaaaaagag gtggaggaat tgtatcaaca atttgaacat tatctgttta aagatttggt
    31681 caagcattct accttgaaac ccgacacatt ggaaacgatc gaacggttga gaaacgctgg
    31741 gatcaatatt ggttccacga atggatttaa tgctcctatg atgacaatcg tagcagaaac
    31801 cgcaaaaaaa gaaaggtatt caccagactt catggcaact tttgaagacg tcgaagggta
    31861 tggtcgaccg tttccttata tgatccataa aaacatgcag tacttcaaaa acaagtcggt
    31921 tgatgaagta atcaaagtcg gagatacggt ctcagatatc caggaaggga aaaatgccgg
    31981 agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg gggatttctt atgatgagta
    32041 ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt ctgttgctct tagattccaa
    32101 caagccggcg ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac
    32161 gctgacatga atccagaatg acttttatac aaaataagag gttgtgaaaa agctgtcctg
    32221 tatcaagtaa taaaaacagc caagcaaaaa tagctcctca tatttttcgt ttggttaggc
    32281 ttattaccgc agatgcaagc ttttgaacac cgtttattcg aaaaaaaaaa gggagctgtg
    32341 acacgacttt tgtcacagtc cccttatttg caaacaaaca gtgtttaaga gacaacctct
    32401 ccactaaacg gtgttcattc agctgactct cggtatttgc tcaaaaaacg ataaaatttg
    32461 aaaagctatt ttctcttttg tttctctaat actcggatca gaacgctgat ttcacattct
    32521 ctttttctta catcttatct ggtgcatgaa gtcctagcaa acgaaggtct tctttcaata
    32581 atacagtcac tgcataaact agagctaagc gtgcttcttt ttgttcatca tcagctaaaa
    32641 tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa tttgattgca tgtttcgcaa
    32701 tcactgacgg ttcgtatttt tccccagcag acaagaccgt ttcagggtat ttttgaacta
    32761 atttaaccac ttcccagctg gtatcgtcat tcaatgcata gtctgctgtt tctgaaggga
    32821 caaatcctgc tttttccaac aggctgactg cacgagcatg tgtatattgc acataaggtc
    32881 cagtttctcc ttcaaatcga acaacttctt ccaggttaaa gtcaaatgta ttcagtcgat
    32941 cgtttttcaa atcgtggaaa atgactgccc caaccccaac ttgtttagct acagcatctt
    33001 tgttttccaa atcagggttt ttctcgctga tttgttcttt agcagattgg attgcttcat
    33061 ttaatacttc ttctaataag acgatctttc ctttacgagt tgataatttt ttaccacctt
    33121 gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga ccagtcaaag cccatttcct
    33181 tcaagacagc tttcaactgt ttaaaatgat agctttgttc attccctact acatatagag
    33241 attgtttgaa gtcgtaagta cgtttacggt acaatgcagc agctaagtca cgtgtaatgt
    33301 atagtgtcgc accgtctgac ttcttaatca acgctggatt caggtcataa gcagataggt
    33361 caacgatctc tgcaccttta tcctcgttca gcaaatgttt ttcttctaaa agtttgacga
    33421 tttcatccat cttgtcattg tagaaagctt caccgttcaa cgaatcgaaa cgtacttcca
    33481 gcagatcata gattttattg aattctttca tcgattcatc acggaaccat tgccataatt
    33541 gaatagcctc tttatcgcct tcttccagac gtttgaacca agcacgagct tcttcattta
    33601 attctggttc tgtttcagct acttcatgga attggacgta taaacgaagc aattcattga
    33661 ttggttctgc tttaacagct tcttctgtac cccatttttt gtaagcgacg atcagttttc
    33721 caaactgtgt tccccagtca cctaaatggt tgatgcggat tggctgataa ccgatttttt
    33781 ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg caaatgtccc attgagattg
    33841 gtttggcaat attaggagaa gacatgtcga ttggtactgt tccttgattc ccgatgttgc
    33901 tatccccata atgttctttt tctttcacta ctgtttgcaa aacttttttg ctgataagtt
    33961 ctttattcat gaaaaagttc aagtaaggtc cgacaacttc gattttttca aagttggcag
    34021 aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg cgcttttcgg taaacttttg
    34081 ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc tgattttgga ttttctagta
    34141 attgctcgac ttgttccagt gttaaatcat ctttgacagc gtcatagaca gcttttgcga
    34201 caagatcttt attattcatc tttattcctc ctaaagtgtt cccttgattg ttaaacaaaa
    34261 aaaatctcta gcgacggcat acttagtatg ccatcactag agacgagatt tcccgcggta
    34321 ccactccaat tgtcctaaaa aaggaccagc tccatctgat atcggtagac gaccgtctgt
    34381 ttaaaacaga attctccaag tgcgcttcgt tgtatcttct tcattcggct tccaccactt
    34441 ccgaattcgc tagccaagaa tgatacaagt actctcttgt tcaacaaatc ttagtcttat
    34501 taaactttct tgaattatat cagaaaaaaa aacgaaaggc tagtcgtttc ataaaaaaac
    34561 aatccagatg gcctaaagga gaagaaatac cacctggata gctataaatc atttcgtaaa
    34621 tacgaaagac ttaacaaatt aatcagctac gataatcgtt ccttcttcag aagctaaaag
    34681 attctcgata ttttctaaag aagtgatgat tgccttagcg tttggttttg cttcaacaaa
    34741 tgcaatagct gcttctactt tcggaagcat gcttcctgga gcgaattgct tttcatcaat
    34801 gtattgtttc atttctgaaa cagtaacagt ttctaatttc ttttgatctg gtttttggta
    34861 attgacatac acattgtcta ctcctgtcaa tacgatcagc aaatccgcat cgatgatttc
    34921 ggctaatttt tctgaagcaa aatctttatc gattacggct tctcgtcctt ctaatccagt
    34981 agctgtttct acaacaggaa tcccaccgcc accaacagag actgtgataa ctccttgatc
    35041 gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg ggtttagggg aagcgactac
    35101 ttttctccat ccacgtccag catcttcttt gaatgtcaca gtggagtcag cattcatttg
    35161 ttctttggct tcttcttccg tgtagaatgg accgactggt ttacttggat ttttgaatga
    35221 aggatcattt tcatccacaa taacttgagt gactaaagaa accacatctt tatcgatccc
    35281 tttttccttc agtacttcgc ccatggcgtt ttgtaaccag taaccgatag atccttctgt
    35341 cattgcaaca catgtatcta aaggcattgc cggtgttttc tcagaatcag cagcttgctg
    35401 ctgaatcaat aggttaccga cttgcggacc atttccgtgg gagatgatca attcatctcc
    35461 ttgttcgata aatttcacta aatactttgc tgtttccatt aacgcttctt gttgggcttt
    35521 tgcacttgca tccgtagaca agatggcatt gccccctaaa gcgacaacta cttttcgttt
    35581 ggccatttca ttcgtccttt cttcagttct accgaatttt ctagataaca aaacgagcca
    35641 gcgttttgtg attttccctc aatgctgact cgttttttcg tgagatgaat gtggttatgc
    35701 ttaaacgcga gggataaaca agttacctaa tgttgctgcc atgatcgctt tgattgagtg
    35761 catacgattt tctgcttggt caaattgacg agcatatttg ctgcggaaga cttcatctgt
    35821 tacttccatt tcagtgatgc caaagcgttc tttcatttgt tcaccgtaaa cagtgtttgt
    35881 atcatggaaa gctggcaagc agtgcaagaa gatcaaacga tctgtgttgt gtgttttttc
    35941 taccatttcc atattgattt ggtaaggttt caacaatttg atacgttctt cgaatttatc
    36001 ttcttcaccc attgataccc atacatcaga ataaaggacg tctgctccgt ctacaccttt
    36061 atcaacatcg tcggtaatca tcaattgtgc accggatttt tcagcaaagc cttctgccat
    36121 tttgacgatt tcttcttcag gttgtaattc ttttggtgca acgatacgca tattcgctcc
    36181 taaaattgca cctgtgacta ataaagagtt agccatattg tttcgaccat ctccgcagta
    36241 agcaacagtg ataccttcta ctgttccaaa gttttcttgg atagttaaaa agtcagcgat
    36301 catttgtgtt ggatgccatt catcagtcaa gccattccaa actggaacac cagagaattc
    36361 tgctagttcc tctaccattt tttgactgaa tccacggaat tcgattccgt caaacatacg
    36421 tcctagtact ttagctgtat cttctgtgga ttctttttta cctaattgga tatcatttgc
    36481 acctagatat tcaggatgtg cacctagatc gatcgctgca gtagtaaatg cagaacgagt
    36541 acgtgtagat gttttttcaa ataaaagcgc aatatttttt ccttctagat agtgatgtgg
    36601 gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa tcgatcaaat attgtaattc
    36661 ttcttttgta aagtcttttt ctgctaataa gcttcttcct tggaatactg attctttcat
    36721 attaaacatt ctcctttaga ttgtttttat ttaagattaa agaggaaatc ccgacggcag
    36781 ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag tcttcacgga caagtggttg
    36841 gctcatgcag cgtggaccac cacgacctcg tgacaattca cttgaattga tttctaacac
    36901 tttgatgcca tagctacgaa gcaattcgtt cgatacataa tttcggttat aagtgactac
    36961 aacacctgga gcgattgcca atgtgtttga tccatcattc cattgttctc gaggtgcaac
    37021 gatctcatct ccattaccag ttgggatcag tacaagatca tctaatccaa gagcagcttt
    37081 catcgtttcg tgtaagttat ctgaatgagt aatcttgata tcatcaccat ctggttcgat
    37141 tgtaaagaca tccatcttac cgtctttact ttgaattgct ggatggatag tgaatttatc
    37201 gtgatctacc attgtaaata cggtatccaa atgcatcatt gcacggttgt tcggaatttt
    37261 gattgccaac actttttcaa atcctgaatt ttttgcgaat aggtggcgtg ctaatttttc
    37321 taaagctttc gcattagttc gttgagaaat cccaacagct actactttat cgcttaagat
    37381 caattcgtct ccgccttcga tatgatctgg attttcacga tccaaccata cttcgattcc
    37441 tttatttgca aatcttggat gatgtttcaa aatatattct gtaaacatcg attcacgacg
    37501 acgtgcttca aaagtcatct tattgactgt catcccattt cccattgatg cggaagggtc
    37561 gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat ggatagtcat cgtctgctga
    37621 cagatcatat agactgctag aacgtacttc gatatctttt gtacgaaccc cagccatgat
    37681 tttatctacc atttcttgcg tttccattga tagaaggaac tcatgaagac cctctcttac
    37741 agcattcgat gcaatatgag attcatccaa cattttattc aagaattgtt ctttgacatt
    37801 gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa agagtctcga cgccttcatt
    37861 ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca atcggaagat atggaatatc
    37921 atcgaacaac agacgatcca taatatctgg tgtcaaattt tccacttctt gtccaggccg
    37981 tttcagtaaa actgtcttta atttccctat ttcagagaaa acgtgaatag gtttatccat
    38041 gttgtgttgc ctccttcttt ttgattacat gtataggata tcctcctttt gtaatcactt
    38101 tcataatttt gtctcattca aaatgagaca ttcctcacat taatattcga taattaaata
    38161 attaaacgtt tttctatttt taagcgtaat ttattcattt tttattctgt atacactttc
    38221 aaaaaaatat ttattcattg aaaaaatttt tttgaataaa ttaaaaaaat aaattatatt
    38281 atcgttgtta gcgtttacta tatgtattat tgttgttttt tatacacaaa tcattcgctt
    38341 caaaacaaat tattcaatac cattgtatat tttttacata ataacaaatt atatttgtaa
    38401 agtattaaaa cttgtttcta atgttggtat gttaaaactt tacaaattct ttaaaaaaag
    38461 tgacaataaa tatagtgaac aataaggaga ggacttttta atgcgtgtta ataaaatcga
    38521 ttttcacttt tcaaaactca gatatcaacc tgattttgaa catttctcgg atgaagagtt
    38581 ccaacagatc aaggaacata ctgttcttcg ttcttataaa aaaggacaga tccttttcga
    38641 cgatggtgat cccagacaac ggttgtatat cttgaccaaa ggactcgttc gtttagaacg
    38701 ttatgatgaa agtgcaactt attactacta tgactatgtg aatacaaata acctttttcc
    38761 tatgtcagga atgtttggag aagagaccta cacttataca gctcaagcga tgactgatat
    38821 tgaaacattc taccttcctg tttcctacta tgaaaaatta gcacgaaaaa atcccgaaca
    38881 gttgatctat atgatacaaa aaatttctaa agtagttgaa atacatgaat tgcgtatcca
    38941 aatcggattg acctctagcg cctttgaccg ggtaaaacaa aatttattca ttttgaaaga
    39001 agaattgggc gtaccaacaa aagatggtca tatcaccatt ccttatccga tcaccttaaa
    39061 agaattagca gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa tcaaacaact
    39121 acgtgattcc gggctgttag attatgacaa aaagcgtttt actttctgta agacagctac
    39181 tactccagct atgagagaat aacgcgaaca aaaataagca gtgaccctga taaatatcag
    39241 ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt agatcactgg
    39301 acaagactca aagattttct atgtgtgttt cagttatgta atattcactg tttgcaaaaa
    39361 atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat tattcgatat
    39421 tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa tgataaataa
    39481 gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat agggctttga
    39541 caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac tcttcggtca
    39601 caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct tgtgaatcag
    39661 agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt gcatacgcgg
    39721 taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg ttcttattac
    39781 ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc ttggatcagc
    39841 attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg ctgctccaat
    39901 cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta cctcagggag
    39961 tttagctgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg ctacacctcc
    40021 agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt tagtaatcaa
    40081 tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta gatcttctcc
    40141 acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc taaaactgaa
    40201 atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt ttccctgatg
    40261 agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc ttccaacttt
    40321 gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat atgatccatc
    40381 ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca tcaatggaaa
    40441 ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt ttacaggaat
    40501 aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa tcagcaacga
    40561 gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg ccgacacacc
    40621 agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat ggcggctagc
    40681 tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag caacgatgtt
    40741 agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac aacttgattc
    40801 aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt tctgctcata
    40861 atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa tatacgaaac
    40921 ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc taaaaaaaca
    40981 tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg tgcatgccct
    41041 tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc tattactttc
    41101 gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata atcagagtga
    41161 ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg tggataagca
    41221 ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc tttttttctg
    41281 caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc atcttctttt
    41341 gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa aacctaactt ccgataaagt
    41401 ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca gcgattcatt
    41461 atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg ctgatatgcc
    41521 ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga accgataaaa
    41581 ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg tgacttcaat
    41641 tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc tcgttcgaca
    41701 tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg acgatcgatt
    41761 tgttcgattg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct ggcatacgct
    41821 ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc atttttcttc
    41881 tgcttctacc cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa ctggttcagc
    41941 taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg gaactgtatt
    42001 aatcatcgca ctaggcaata tttcctttat ttcatagaag aatttgtgac aatcactccc
    42061 gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag aagcagaaat
    42121 atgtacatct tcaagtacaa tttctaatct attttctttc cagcggtatg ccccagcgta
    42181 tacatttttt cttcgagcat caaacatggg cacaataatt ttatccacac cgatgcaatt
    42241 agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg tataagcaat
    42301 cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac catcagaaac
    42361 cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt ggtcgattgc
    42421 aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc caagaatttg
    42481 ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc ctaatgtaat
    42541 catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat acttttttat
    42601 tctgaggatg aaaataccaa aggcaaaact agtacaatga tacatgatta tctgctggcg
    42661 ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcacttca tttccccaca
    42721 gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc acaataatct
    42781 ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact gctagctctc
    42841 ccatccattc ggtataaact tttacagtag aaagagaggg cgttacatat ttcgctacac
    42901 tgacatcatt gaaacccatc acagaaatat cttctggtac cactaaacct ttttcttgaa
    42961 tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca tcaggaacat
    43021 gcggattcgt ttttaaatac tgcttcactg cttgatatcc tgactcgaca gaaaaatctg
    43081 cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga aatgccataa
    43141 ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca gcaagcatag
    43201 cgatatgttg cgctttatgg gagacaaaat gttcaatgac tttttgaaca cttgacttaa
    43261 agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc aaaagcaagg
    43321 cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct agtgcgatcg
    43381 ttccatctgt cggaagatca ctaatggaat cccacgtttc tttcaataaa gtaacgtttg
    43441 cttcttctgc ctttttttcg attcctagac gaatagataa ataataaaga tcttcaagct
    43501 cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt gctgcatttt
    43561 tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc tttgtggcta
    43621 agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca ggagaaactc
    43681 ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt tttacgttta
    43741 taaaattgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct tgaatactcc
    43801 agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga catcggtgac
    43861 cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg gacgatgata
    43921 ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta gttctggttt
    43981 caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt tctccttttt
    44041 gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt ctgaaacatt
    44101 gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg caattggcgt
    44161 aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg tttcatccga
    44221 atatgctttc cacttttcca atactgagtt agctgcttca atcaacaatt ttttctctgt
    44281 tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga cttgcggata
    44341 atctgctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt catgacgtcc
    44401 tgcctgataa tgatcatgag aaagaatcga tcctccaaca atcggcaagt cagcattcga
    44461 accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa atgtttcctt
    44521 tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat tgtaataagc
    44581 ataaggagaa tattggaatc cccagctttc accgtctaga ttcattcgaa taatccggtg
    44641 attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca tgcaaagcat
    44701 gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt ttggatcttt
    44761 ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact ctgccggaaa
    44821 tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac ttaaattaaa
    44881 gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga atgcattgac
    44941 gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct cttgttctgc
    45001 tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat ccaataagtc
    45061 tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt ctcctaccat
    45121 ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc cagcttcgat
    45181 cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt cccctattct
    45241 tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat tgctttgata
    45301 tctgtgtatt caggtttcca acccagaacc tctctcgctt tttcacttga agcgactaat
    45361 cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt tcctgtcact
    45421 tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc taaattgaag
    45481 aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc agcaatcaaa
    45541 tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata atcgtctcca
    45601 taaacagcca atgcttttct ttgaccaaga gctacttgta aaatgattgg gacaagatgc
    45661 gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc tacattgaag
    45721 tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat cattttttcc
    45781 atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc ttctgtgatt
    45841 ggagactctt ttggttctcc gtaagttgct gcagtagaag agaacacgat tttattcaca
    45901 ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt gttgaaatac
    45961 atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg gattactcct
    46021 tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat gtctccttca
    46081 taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt atctaccaca
    46141 gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat ataaccggct
    46201 ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat tgtgtttgtt
    46261 gattatatat aatttaaaaa aacgaagttt acttttatgg tgaaaacaaa caaagtaaaa
    46321 tttcgtaaat tcatatggta aatttataat aaaacattta gtaaacattt tcaaggattt
    46381 tttgcaaagg tttacaaaca aacgtaaaac tagccttttt ttctcttttg ttccataata
    46441 gatataacaa ataataaatg aggtggattc aatgaaaaaa tttgtatctg gaatattagt
    46501 aggaagtctg gcaacagccg ctgccgttgc tggtttggtc gcttctgtga aaaaaacagt
    46561 gatcgatcca attgacgaaa aagaagcaat gatcgaagaa aaccgtaaaa aagcaatgcg
    46621 caaacgcgtt tctcgttaat aattatttaa aaacacgcat gaagacttgt gaaagaacag
    46681 ataagtcatc atgcgtattt ttatttgtca gtcaatataa gttgtgctat tttatctgcc
    46741 tgtctcagat tccatggatc tgtataaggt ctagctactg ctgcagttag ccaatccaca
    46801 ccttcaacag gtattccata gcaataaaag tcagactgga tttcccctga agcatcaatc
    46861 acgcgattcg tacctcgctc aacatctata gcttctgaat gataacttcc tgtattatct
    46921 gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat tttttatgac aggattactt
    46981 gaacgagaaa agcttgtttg tggcaatcga gcttcaatca aatagcgact gctgatttct
    47041 ttatcttgta gagaaataac atagcggttg tgcttttgct gaatatcaaa tggcggttcg
    47101 aacaaatgaa aaatacccgc atctatcagt gctgctaatt ctttcgtccg aatgactggc
    47161 ggaccgatcg ttaaaaagct atttagatga gtaaaccatc cccatagtat ttctttgtat
    47221 tcctttgcag aaaaaacgtg ccattcaata gcctgccgga ttggatcacg ccagtctttt
    47281 aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc ctttttccgc ttcttttatc
    47341 tgcccttcaa taaatgctcg gctagcttct gcaaaggact catcctgata ggtaagagga
    47401 atttctaaat acgaccaatc ccatctataa gggattagtg ctggatgctt tccgagacac
    47461 tcctctgaag agagggagag aaaatcatgc tcaaaatttt tctttggaat cggcaaatga
    47521 tattcttcga tgattttctt gtaataaaac aactcaacgt cttttttcag atagtcaaaa
    47581 aatgtttcgc cagtcaaaaa tcccgactga tgccattgct ccaggttttc ttttgttagt
    47641 aaccgtggcc atgccattga tccgccttgc ttttggtttc ttcctcgagg gtaataaggg
    47701 agcccccttc ttgatccact gtatacaatc ggttccttgc ctgatggatg atagacaagt
    47761 tttccttttt cgttctcgaa gcgcccttct cttgatactg taaacaatcc gacataatca
    47821 aaaaaactta agcctagacc tcttaaaaag acattttctt taggcggtat cttcgaaaga
    47881 tcgacatctg caggattaga aggaagctga tacagtaaat catttttctt tgcgtagtct
    47941 acccacactc tttcttcttc tgtctcatca ttttcccagt gaccgcttgc taaaacaagc
    48001 tgatcgatag aatagctttg ctgatctgtc tgtaaaagat aaccatcttt tcttttgctg
    48061 acttttttta ctaaagaata aacaaactga atagaagctg gaaattcttc ttgcaacttt
    48121 gagaaaaacc agcgttgata gagtccgtat aaacatcgag tagattgctg atctttttcc
    48181 agacgattga tttcttctat aattagttgc ttatttttgg ttaccgtctg ttcgatataa
    48241 tctgtagcag gtcctttact ccattgaaac aagttgggtc cctgtgttac caatcctcca
    48301 ctggacaatg tttcatctgt aaaaagcgtc acttgttgag aaacagaatt cattaataac
    48361 tccggtggct gattcaaccg ccaaactcga cctccaggac cattgggatc gaaaagaaaa
    48421 agttcaagag aatgtctgtc ctgattatta tggagaatcc tttctgctat acttaatcct
    48481 ctaggtccgg caccaatgat ccctattttc atttgcatgc ctccatttcg ataaattttt
    48541 tcaataaaac agcatggttc tgatgttcat tttttgcacc ataaacaaaa gtaatgactg
    48601 gatgttcttt cataagttga ctgagtttaa aaatagcttg cttcttttct tcttcgaaaa
    48661 tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg ttctggtaca tggtcaaaag
    48721 ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca cagatctaac tgcgcttttt
    48781 cttttgaaat tcctcgtggc cagaacctat ccacgagtat tctatagcca tcctcttttt
    48841 cagcatcaag gtaaacacgt tttaatttga tcatagttgt ttccttcttt ctgccttttc
    48901 gcttagctta tatatgtaga taatagcaca aaataaaaaa aatgaacggc acaactatcc
    48961 attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt gattcggttt ttaaagctgt
    49021 catacaatca tctgttttat acttttttac ttttgatttt tccagaccat ccgtcatatc
    49081 cacccttaag taaataaatc tcttgataac ctgctttacg taatttattt gctgtacgga
    49141 tgctcaagct tttcttttga tcgtaaatat agacaggctg gtcttttcga atagatccta
    49201 atgaatcttt caaaacagtg taagggatgt ttcttgcacc taaaatatgc ccagcatcaa
    49261 acacatcttt ttcgcgaaca tcgattactt gtgcttttct cattccttct cgaaattctt
    49321 cttctgtcaa agtcgttgct gaacgcttgg ccattactcg tagatagact tcccaaaaga
    49381 tgattgcaag cacaatcaat agcaaaatga tattaattac ccataaaacc atgtaatgcc
    49441 tccgtttttt taagcaaatt tcaaggctaa tgaggcagca ccaatgacac cagcttcatt
    49501 accaagctct gctaatttga tttttgttga ttctcttact tgcgggaaag taaattcttt
    49561 aaaatatttc tctacacggc tacgtaaaaa ttctcctgct gccgatactc ctccacctaa
    49621 cacaatactt gacggattca atgtattccc caagttggcg caagctaaac ctaagtagaa
    49681 gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct tcttgagcca tttcaaagat
    49741 atctttactt gttacttctt caccgttgtc taacattgct ttcagttttg aatctcctgc
    49801 gtattcttct gccaagtaac gacctagacg taccacacca gtagcgctag acacagtttc
    49861 taaacatccg cgtttaccgc aagtacattc gaacccttca ggatccacag tgatatggcc
    49921 gatctctccg cctgctccgc tcacgccgtg aatcaaattt ccgccagcaa cgataccacc
    49981 gccaacacct gttcctagag taacgaatac aacgtctgga tcgttttctc cagcaccttt
    50041 ccatcgttca cctaaagcag caacattcgc atcattatcg atagcgaatt tgattcctgt
    50101 tcctgcctcg atcagttttt tcacaaattg aacttctgtc cagtttaagt tatacgcacc
    50161 aataaccgtt ccagcttcgc tgtccacgct tcctggggta cccatgccga ttccgataaa
    50221 gtcttcggct ttcatattat ataaattcaa acgatggttg attgattcaa tgatttcagg
    50281 aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg ctccattttt gttgaatctc
    50341 tccttctggt gttaaaattg caaattttgc tgttgttcca cctaaatcaa tcccaataat
    50401 cttcttgtcc attacaaatt tcctttcttt cggctttctt caattcggtg ctctcttttt
    50461 aatatgcgcc acgctgtcat gtatgtatca tgatcaatca aacggctatc atacagatta
    50521 cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg cacccacata gataaagatg
    50581 ccaaattttt tgaacagctg ctgtacatca tataagctct ccatctttaa gcacttcctt
    50641 catattatac aagtaaacca tattttttta aacccagtaa aatacatatt acaaaaagaa
    50701 atacaaaaac tagtgtagaa agaatccttt ggtgtatcga atagcttgtc tgttgtttcg
    50761 gtagacccaa aacattccct aacactaatc cgccaattag cccaccgaca tgtccccaaa
    50821 catctacaga ggtatccaga agaccaaaaa caaatgtcaa cacaataaat aaaagaaaat
    50881 gtctgactag ctgctggatc gctgggttat gtttaaaatg aaaccctaag ataaacaacg
    50941 caccgaacaa accgaatatc gaagtactcg caccaccaga cagaacattc gcttcgttaa
    51001 aagcaaagct tgctgtattt cccattatcc cgctgaacat ataaatcaag aaaaaacgcc
    51061 aatggccata gattgcctcg atttgctgcc ccataaaata cagtatcagt gagttcatta
    51121 cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac taaacgccac cattcattga
    51181 agtgaacaag ataaggacca tacattccgg cataataagg gatctcaagt cgcggaaata
    51241 attccatcat gaggaagacc gctgtctgta tccctaaaaa agcataggtc cataacggtc
    51301 gtcttaacca tgcacgcaat tttatttgct gttgataatt catgaaaacc tcccatttca
    51361 ttttagtcaa taaacaattg ttggatcttc tgatcaaagc tctccggttg ccattgttca
    51421 ttcaattgtt cacgaaaaac aaggctacat gtttttttat gaaaattatt taaataccgg
    51481 tcgtaataac caccgccaaa accgattcga taaccggctc tattgtagat aatacctggt
    51541 acaatgagga gatcgatcat ctccggcaaa tagagcttct ctgattgtgg ctcaagaaca
    51601 ccaaaactcg aacgtacaag aacggactgt tcatccattt cataaaacgc catctgacgt
    51661 ttcggcaaag ttttaggtac aactattttc ttttgttctt tttctgctcg ttggataacc
    51721 ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc ctatcacttt tgctgacttc
    51781 cagtactcgc tggcgaatag ctgtttatag attcgctgtt ctttttcctt tttcttgcca
    51841 cttttggcca gatttttcag cttttcgatt cctttccggc gtaatcttac tttcaattgg
    51901 tccaaaaact cacccttcct tctctcataa aatggtatag gaaaaaaaga aaaatagcaa
    51961 tcgaactttc aaataaatgc ataaatagaa taaaaagaag acctagaaat gattggcctt
    52021 ccttttttaa taaatttcaa ctgttcagcg cgtgtttttg tctgataaaa agtgcataca
    52081 gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt aggtacttcc ccacttggta
    52141 gaatcactct agcaaccata tctgcactaa gcagaaaaat ggaaccgaac aaaccgctca
    52201 gaacaaaggt ccagttattt tctttacgta cgactagctt cgcagcatga ggagctagta
    52261 gtcctacaaa agataaacta cctgtcaccg ctacactacc gcttgccaaa gcaacagcta
    52321 acaacaaaag gattttttga cttttagcca cgttaaggcc taatccttgc gcgcgttctt
    52381 gaccaaatga tagcaatcca atcctctgtg cataaagtcc tcctagcgga attaagataa
    52441 ccgcccatgg aagcaatgcc caaacataag gccagctcgc accccagatt gatcccgcta
    52501 accatgcatt cacaaatcca taattttcct tagataattg gatcgtgcat agcaatgtta
    52561 atgcagaaag gccggcatta acggcaatac cggacaataa gattcgattc atacccaaaa
    52621 agccatttcg atcataggcc gcaagataga cacagcaagc agctaaaaca ccacctagaa
    52681 aggctacaac tagcaataga aacggcagcg aatagtgcga aaagaaacct aaataaagca
    52741 tcactaaaaa tcctgctccg gcattaatgc ccaatatact agaatctgcc aattcatttc
    52801 ttgttactcc ttgcaagata taaccagaaa cgccaagaca agctcctgct aataaactga
    52861 tcagtaatcg aggaactcga aattctctaa caatcagcgc caaagaagat gattctttcc
    52921 ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg cgtaccaatc attaaactac
    52981 ctatagacag gatgacaagt acaaaaagca agaaatagaa cgtccaatat atttttttca
    53041 ccgttgttcc ctccttactt gtatcagtaa aaacggaacg ccaatcaatg caatgactaa
    53101 tccaaaaggt gtttcaaaag ggggattgat catcctcgct gtaaaatccg ccaacataac
    53161 caatccaccg ccaactaaaa aagagacagg tagaacataa cgatagtccg ttccaaccat
    53221 tccgcgaaca actggcggaa ccaacagccc aataaatgaa acaggtccga ctaacgaaac
    53281 ggcaatggct gctaacactg caactagaca gaaaacaatc aaccgcgttc gtttaggatg
    53341 acttccaaga gctaatgcat gtgtttcact caaatgaatc aaagatattg cttttcctaa
    53401 tgaaaaagaa accaaaacag caagtatata taaaggaaac aatactgcta attgtttcca
    53461 tgtgatattg gcagctccgc ctacaaacca aaaagcaaga tcctgctgca gattaaagaa
    53521 ttgggtaagt gcttggctga tagcagtaaa aaaagaacta atagctacgc cagctaaaat
    53581 cagctgtaaa ggattcatgc ccattttcga aaactgcaaa aaaccaaata cgagcaaagt
    53641 tacagcaaaa gaacctgcca atgacattag aaacacagaa aaatcagaag ctttaggcca
    53701 aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg ttgattccta gtaagcctga
    53761 atctgctaaa ggatttcttg tcactccttg catcaatgca ccgctcaatg caaaacaact
    53821 ccctacaaga aatgctcccg ccacacgagg caaacgaata tttcgtatga tttggtgact
    53881 ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa tctgcttttc ccacagtcgc
    53941 tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga acaaaacaga ccaaaagcag
    54001 gagtctattt ttcttcatag tcctcataat tgatcctcct gctcttttgg caacaaatca
    54061 taggttagaa aaactggttt atttgttctt ggaagcgtga caatcgttgc atcaatctca
    54121 aatatttttt tcatacactc ttttgtgatc acttcttcag gtgtaccttc tgtgatcaac
    54181 ttaccgtttt tcattgctaa taaataatct gaaaaacgtg aagcatgatt aatgtcatga
    54241 atcgacatca agatcgttgt ttgtttcatt tgattgattt cagtcaataa ctgtaggatc
    54301 tctaattggt gtgccggatc taaaaatgtt gtcggttcat ccaagatcaa gatatctgtt
    54361 tcctgagcaa gcgccatagc gatccagaca cgctgggttt gtccaccaga cagcgttgcg
    54421 atatcccgca tacgaaatcc agctagacct gtagcatcca acgcccactc gattgcttca
    54481 tgatcttgcg tgtccatgcc actgaacagt ttttgataag ggtatcgtcc gtaagatact
    54541 acttcttcta ctgtcatacc tgtagggtgt tcactagttt gtgctaataa tgtaattttt
    54601 tttgcaagtt cttttgaact gtatgtagag atatcagagt tattgacaat gatctttcct
    54661 gatttcacag gtagtatacg ggtaaaactt ttcaataaag tagattttcc acttccattt
    54721 ggcccaatca aactagtgat tttcccttca ggaatcgtaa cagacatgtt ttcaatgatt
    54781 tttttgtttt cgtagccggc tgcaatgtca gctgctctta atctcgtcat ttcacccatc
    54841 ctttcgctga aaatcattct caattatgac gctgtttcaa catttcgtca agcgctactt
    54901 tcatatctat agccaaatga agcggttgac agcgttcgaa atatcaggaa taataaccaa
    54961 tgagaaagat tctcaattat gttaggagga aagaaatgaa attaaaaaca aaagtattca
    55021 cattatctag catattgctg ctggctggag tactgagcgc ttgctccccc tcttcagata
    55081 aagaaacagc tacaactgcc aattcgacga cttcatccaa tagtactcat acagtcacag
    55141 atacacttgg acataaagta gagattccaa acgcgcccaa acggatcatc ggcagttact
    55201 tagaagacta tctgattgca ctaggggaaa aacctatcgc ccagtggact gttggcagtg
    55261 gttccattca agattatctg caagatgatt taaaggatgt cccaacgatt tcttatgacc
    55321 ttccttatga gaaagtgtta agctttgagc ctgatttgct gttgatttct tcttccgcta
    55381 ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc gccaacttat gttgtaaaaa
    55441 acggaacaga tgtcacatgg gaagaacaac taaaagatgt cggcaaagct ttggcaaaag
    55501 aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc catcaagaaa actcgagaag
    55561 aactagcaga taaaatcgag aacaagacag ctgcagtgtt gtgggtgacc aacaactcag
    55621 cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt atacgacgat ctggaatttg
    55681 gtgttcctga tctagtggaa gaggtctcca aaaaagccac ttctgactgg tctgctgttt
    55741 cctctgaaaa gctagctgaa ttagatgctg actacatcat ccttgtgaac agtgacaaag
    55801 atgctgccat gttcaatgaa ccaagctgga agaatcttaa agcagtgaca gacaatcatg
    55861 tactgcaatt cggacctgaa tctagttggc tatataacgg acctattgcc agtcaggaaa
    55921 tggtcaatga tatcaaagaa aaactaaatt gattttaact aaaaaaagca tcttcacgat
    55981 tagtcgtgag atgctttttt tcttattaac aaactagacg catagatttt attcttcttt
    56041 tgaagcaacc ctatttttta ctctttccca tttttgaaaa cgatgggcat atagattttc
    56101 ttgatccgtt atcccctcat actcctctat aagggcgaag tctgaaaaat caatattccc
    56161 aatatacgta tcgccttcaa acgtgctatc gatcaatgtt cgccagatcc gaccagtgta
    56221 aggaagaagt tcttgaaaaa tccgactacc acccgaaaca tagatatctt ctgagatcgt
    56281 ttgactatac gacaaaagtt catcgatcga atgcattact tccgcatttt ctttcgtgcg
    56341 aaaatttggt tgcgttgtca acacgatgat atggcgataa ggaagtgaaa gatcccccat
    56401 tccttcatag gtctttcttc ccatcactaa aattcgatcc attgtatgtt cgcgaaagaa
    56461 acgcatatca tttggcaatc gccatggtaa caaaccattt ttgccgatca gaccattttt
    56521 atcttgtgcc cacatggaaa taaacatatt atccgctcct tatgatttta gatgttttga
    56581 gataaaaaaa gtctcaattc tttgaccagc aaggaattga gaactttaaa aacattattt
    56641 tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa tatttttgtt tttccaaacg
    56701 atcaggattg ttacgtttgt ttttgcttgt tagatagtta cgttctttac aagaagtaca
    56761 ctctaaagtg atgtttacgc gcatgttttt ccctcccatt tttacaagaa tcctctaaga
    56821 tgactctcag acttgattat cttaccatga ttttttctag attgctagtg tttttcacga
    56881 ttttgttaag taaaactact tgacgataaa tacaacggtt ttacttctca aacaaaatct
    56941 ttttcttttt gtataaatac ccttacactt gtttatatag attatgtttt ttaataaagg
    57001 gggatactaa tatgccatgg aacatgaagg actttcctgc atcaatgaaa aacttagata
    57061 aattgacaag aaaaaaagca atcgacatcg caaatgcatt attagatgaa gggtaccctg
    57121 ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa atggtatgaa gatgctagtg
    57181 aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag caagacggat gaacacgata
    57241 cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat cgtagaatat gaggaagaac
    57301 aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa ccattttgat acaaaaaaag
    57361 aagcaatcga aaaaggaaag caagtagcac aaaacaaaga atcaactttg ttcatttata
    57421 aaaaagatgg gacaaaagaa aaagaaatcg attattgaac atatgtaaag gggttgtgac
    57481 gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt gttcctctgc caactccgaa
    57541 ccataagtca taatatctat aaaacgggaa gagctgtttt ttgaaattgt ttcttatcac
    57601 tcagtgttaa acggtaggag aaaacctctt ttttattgat atttgatttt catcaaacag
    57661 tcacatagtc gattgcttct gcttgcggaa tcgtttcatc tacaaagtag actttatacc
    57721 atgtcaagaa actgaagatt gtccagattt ttctttgctc ttctttacgt ccttcgtgat
    57781 ggtcatctag caacttcatg attttttctt gatcaaaaaa ttcttttgca aaatctgctt
    57841 cgaataatgc tttgacttgt tcgtagccgt gttcttcttt caaccaagct ttgattggca
    57901 cagggaatcc taatttcaca cgattcgacc attcttccgg aagatgtttg ttcgcagctt
    57961 gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa cttacttggt atttgttgag
    58021 ccaatttcat gacttcgata tccaataaag gtactcggac ttccaaagaa cttgccatag
    58081 aaagtttatc tgcctttaac aagatatctt ttggcatcca ttgatgaaga tcgacatatt
    58141 gcatcttatt gacttcatct ttgattcctt ctgttttctc gtaatgaaca gtcatgattt
    58201 catccacaga tggtgctgtt tgatattttg gtgttaacac ttctgatgct tctgattctt
    58261 cgaacactcg agcatgaccg ataaaatatt cttttgcagg agcaagtgat tcatataaat
    58321 ggatccgtcc atggaaattt ttcatttttc cgattttacg tcctaaatta tagcgtgttc
    58381 cttttggtaa ttttttcaat ccttgagcaa tcacacggat gaattttgaa ttcgtatgga
    58441 atccatacgt ttggtatccc gcaaacagtt catccgcccc ttctccagat tggactaccc
    58501 gcacactttg tgaagcaaga tttgctaaaa agtacaaagg tacgcaagaa ggatttgaat
    58561 caggttcatc taagtgatac tggatcaaag ggaatgcttt gaacgacatg tctccgtcga
    58621 tcactgctgc tgtattatct aagtccaata gttcggttag ttttctggct tcaatcgctt
    58681 cattataggt tttatcatca aacccgattg agaaagaatg atctggtttc aatacagagg
    58741 ttacataact ggaatcaacc ccgctggata agaaagaacc aacttctaca tcactgaccg
    58801 tatgtgcttc aatagaagct tgaacggttt cgtcgatttt ttcgatccat tcttgtcggg
    58861 aatgcgtttc ttttgtttca aaatctgcat cccaatattg ctgaatgtct agttttccat
    58921 ctttatacgt ataataatgg ccttcaggca aacgatagac atctttgaag aatgtttcgc
    58981 cgtttaatgg agaatattgg aaagtcatat aaggtttcaa cgcttctttg ttcaattctt
    59041 tgttaaaatc aggatgaggc agaaaacttt tgatttctga accaaacata aatgttccgt
    59101 tcatttctgc ataataatat ggtttgatcc caaagtggtc tcttgcaccg aataactcat
    59161 tcttttcatt atcccaaata gcaaaagcaa acattccgcg gatcttttgc aataattctg
    59221 ttccccattc ttcgtaacca tgcaaaagga cttctgtatc tgcatgtgtt tgaaaaacgt
    59281 ggccagctgc aatcaattct tctctcaaag gctggtaatt atagatttcg ccattaaaaa
    59341 taatgatctt tgtccgatct tcattgtaaa taggctgagt accgccctct aagtcaataa
    59401 tacttaatct tctgaatcct aaagcaacat gtttatcgat atattctcct gaactattag
    59461 gtccacggtg aacgattcga tccatcattg tattaataat tgtttttttg ttgtccttgt
    59521 cgttaacaaa acctacaata ccgcacattt aactttcaca tcctaatata aaattactgt
    59581 ttatttcatt catatgcatc ttatcataga ataaatagcg gacgaatatt ttctctctta
    59641 caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt tttacagaaa ataaagtccc
    59701 aatatctaat ttaccatgtt ttcttttttg aggctacctt attctcttcg tgttaaaaaa
    59761 atattagtgg atgctatttc caaaaaaaac agcaaaaaaa gacacgacct aaagatcgtg
    59821 tctaattatg actgtatcag aaaatatgat accaaacgga tctattcatt aattaattga
    59881 catagtttat ctgttgctct tttatgactg gataactgac caattcatga cgatccaagt
    59941 cttctttatt tatatttaca gcagtaattt gactgtcttc atacgtacga taataataaa
    60001 tccctttatc aacgttacag caagaagaat aaatcgtata ttcgtatttt ccttctccag
    60061 tatcgcatac tcctttttgt tgctcgactg agcctagtat atgaaaaaat tgactgatac
    60121 tttcagattc tgaatcacct gatgcagcat tcattttagt aaatgtggct tttacaaatc
    60181 gagaaacaga agagagatct ccaggtaagc ccaatcctcc catcccacga ctataaacat
    60241 tcaagctgat ctggtttgaa aaatgatttt ctggtgttcc atttgatagc gagcgataat
    60301 tgttcagatt aaataattga taatcaaatg gcggattatt tgtcaacaca ccgacaggat
    60361 tatcatataa atgaagtcca tctttcatac tttcaattac tatcgatttt tctttatcag
    60421 ctaataacca gtgtaaaggt gacaagggta gtttttcact aaaatcgata ttgactagat
    60481 tgatagtagc caataattct ttagcttcgt ctatggttga gcattgccct agtatccatg
    60541 gaataaactc aaatggtgca acgttatctt ttccttcttg taattcttta taatcagcat
    60601 tgccaggaaa attcagtcct gccatactca accctttttc atttgtagcc tcataataga
    60661 gtggatagtt ggatacccca gctgctattc ctatcattgc ataatgttta tctaaatttt
    60721 ttacctttct aaaatcgaaa cgataatttc ttggagtaat agtgactact tcattataag
    60781 acatctcata atcaaaattc cttccaaaat aatggtcttt cgttgcataa gtgattgcgg
    60841 tacacatcgt tttccctcct aaaacaaatc gtttttacac ttttatctta catctagtat
    60901 ttttttttat caaaaaaaag gccaaagaag attgatttac tagcatttat aaaaaatagt
    60961 actcaggggg aatcaaaaaa tattgtaggt taaaagtaca acaataaggg gctgtgacaa
    61021 aa
//
LOCUS       DAPBBG010000011        59936 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPBBG010000011 DAPBBG010000000
VERSION     DAPBBG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 59936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.011"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..196)
                     /locus_tag="RD477_000797"
     rRNA            complement(<1..196)
                     /locus_tag="RD477_000797"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(719..2029)
                     /locus_tag="RD477_000798"
     CDS             complement(719..2029)
                     /locus_tag="RD477_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDT7633178.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2041..3459)
                     /locus_tag="RD477_000799"
     CDS             complement(2041..3459)
                     /locus_tag="RD477_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7633179.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3699..5267)
                     /locus_tag="RD477_000800"
     CDS             complement(3699..5267)
                     /locus_tag="RD477_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDT7633180.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5271..5681)
                     /locus_tag="RD477_000801"
     CDS             complement(5271..5681)
                     /locus_tag="RD477_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDT7633181.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5678..6601)
                     /locus_tag="RD477_000802"
     CDS             complement(5678..6601)
                     /locus_tag="RD477_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDT7633182.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6598..7476)
                     /locus_tag="RD477_000803"
     CDS             complement(6598..7476)
                     /locus_tag="RD477_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDT7633183.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7463..8800)
                     /locus_tag="RD477_000804"
     CDS             complement(7463..8800)
                     /locus_tag="RD477_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDT7633184.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8803..9906)
                     /locus_tag="RD477_000805"
     CDS             complement(8803..9906)
                     /locus_tag="RD477_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7633185.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9980..11488)
                     /locus_tag="RD477_000806"
     CDS             complement(9980..11488)
                     /locus_tag="RD477_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7633186.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYDVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11454..12146)
                     /locus_tag="RD477_000807"
     CDS             complement(11454..12146)
                     /locus_tag="RD477_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7633187.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12168..13142)
                     /locus_tag="RD477_000808"
     CDS             complement(12168..13142)
                     /locus_tag="RD477_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633188.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13442..14314
                     /locus_tag="RD477_000809"
     CDS             13442..14314
                     /locus_tag="RD477_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7633189.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14772..16970)
                     /locus_tag="RD477_000810"
     CDS             complement(14772..16970)
                     /locus_tag="RD477_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633190.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            17202..18194
                     /locus_tag="RD477_000811"
     CDS             17202..18194
                     /locus_tag="RD477_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633191.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18254..19675
                     /locus_tag="RD477_000812"
     CDS             18254..19675
                     /locus_tag="RD477_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDT7633192.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVCNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19668..21167
                     /locus_tag="RD477_000813"
     CDS             19668..21167
                     /locus_tag="RD477_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDT7633193.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21206..22600
                     /gene="uxaC"
                     /locus_tag="RD477_000814"
     CDS             21206..22600
                     /gene="uxaC"
                     /locus_tag="RD477_000814"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDT7633194.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22759..23442
                     /locus_tag="RD477_000815"
     CDS             22759..23442
                     /locus_tag="RD477_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDT7633195.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23444..24280
                     /locus_tag="RD477_000816"
     CDS             23444..24280
                     /locus_tag="RD477_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDT7633196.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24291..26045
                     /locus_tag="RD477_000817"
     CDS             24291..26045
                     /locus_tag="RD477_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633197.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26321..26662)
                     /locus_tag="RD477_000818"
     CDS             complement(26321..26662)
                     /locus_tag="RD477_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7633198.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26730..28901)
                     /locus_tag="RD477_000819"
     CDS             complement(26730..28901)
                     /locus_tag="RD477_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDT7633199.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            29070..29969
                     /locus_tag="RD477_000820"
     CDS             29070..29969
                     /locus_tag="RD477_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7633200.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(30040..30897)
                     /locus_tag="RD477_000821"
     CDS             complement(30040..30897)
                     /locus_tag="RD477_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7633201.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30882..33584)
                     /locus_tag="RD477_000822"
     CDS             complement(30882..33584)
                     /locus_tag="RD477_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDT7633202.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33597..34886)
                     /locus_tag="RD477_000823"
     CDS             complement(33597..34886)
                     /locus_tag="RD477_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDT7633203.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            35036..36082
                     /locus_tag="RD477_000824"
     CDS             35036..36082
                     /locus_tag="RD477_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633204.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            36084..38237
                     /locus_tag="RD477_000825"
     CDS             36084..38237
                     /locus_tag="RD477_000825"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDT7633205.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38710..40161)
                     /locus_tag="RD477_000826"
     CDS             complement(38710..40161)
                     /locus_tag="RD477_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7633206.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(40158..41882)
                     /locus_tag="RD477_000827"
     CDS             complement(40158..41882)
                     /locus_tag="RD477_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7633207.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41875..42489)
                     /locus_tag="RD477_000828"
     CDS             complement(41875..42489)
                     /locus_tag="RD477_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7633208.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42834..44291)
                     /locus_tag="RD477_000829"
     CDS             complement(42834..44291)
                     /locus_tag="RD477_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7633209.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44332..45252)
                     /locus_tag="RD477_000830"
     CDS             complement(44332..45252)
                     /locus_tag="RD477_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7633210.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(45264..46211)
                     /locus_tag="RD477_000831"
     CDS             complement(45264..46211)
                     /locus_tag="RD477_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633211.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46690..47409)
                     /locus_tag="RD477_000832"
     CDS             complement(46690..47409)
                     /locus_tag="RD477_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDT7633212.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47458..49104)
                     /locus_tag="RD477_000833"
     CDS             complement(47458..49104)
                     /locus_tag="RD477_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7633213.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49283..49873
                     /locus_tag="RD477_000834"
     CDS             49283..49873
                     /locus_tag="RD477_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7633214.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49965..51470
                     /locus_tag="RD477_000835"
     CDS             49965..51470
                     /locus_tag="RD477_000835"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDT7633215.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51554..52906)
                     /gene="celB"
                     /locus_tag="RD477_000836"
     CDS             complement(51554..52906)
                     /gene="celB"
                     /locus_tag="RD477_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDT7633216.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52906..53424)
                     /locus_tag="RD477_000837"
     CDS             complement(52906..53424)
                     /locus_tag="RD477_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633217.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53440..53766)
                     /locus_tag="RD477_000838"
     CDS             complement(53440..53766)
                     /locus_tag="RD477_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDT7633218.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53779..55758)
                     /locus_tag="RD477_000839"
     CDS             complement(53779..55758)
                     /locus_tag="RD477_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7633219.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55779..56093)
                     /locus_tag="RD477_000840"
     CDS             complement(55779..56093)
                     /locus_tag="RD477_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDT7633220.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            56261..56554
                     /locus_tag="RD477_000841"
     CDS             56261..56554
                     /locus_tag="RD477_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDT7633221.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56632..57744)
                     /locus_tag="RD477_000842"
     CDS             complement(56632..57744)
                     /locus_tag="RD477_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7633222.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57897..58121
                     /locus_tag="RD477_000843"
     CDS             57897..58121
                     /locus_tag="RD477_000843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633223.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            58131..58310
                     /locus_tag="RD477_000844"
     CDS             58131..58310
                     /locus_tag="RD477_000844"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633224.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58504..58674
                     /locus_tag="RD477_000845"
     CDS             58504..58674
                     /locus_tag="RD477_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7633225.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(59098..>59936)
                     /locus_tag="RD477_000846"
     CDS             complement(59098..>59936)
                     /locus_tag="RD477_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7633226.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT        17892 a        12259 c        10494 g        19291 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttatt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcgagag tgtttcccga
      361 ttcatttttg gctgccgtag caacttttat atcatatcag agagacaaac gattgtcaac
      421 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcagcaac tcatttatca
      481 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      541 ttcgcaagtg gttcttttta acgacttagt tatcttatca cgtctaaaaa catgtgtcaa
      601 ctcttttttg gaattttcat aaaatgtttc aatgaagcgc gacaacgttg tttaatatat
      661 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
      721 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      781 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      841 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      901 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      961 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
     1021 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
     1081 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
     1141 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaatcga
     1201 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
     1261 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
     1321 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
     1381 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
     1441 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
     1501 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
     1561 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
     1621 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1681 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1741 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1801 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1861 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1921 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1981 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     2041 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     2101 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     2161 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     2221 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     2281 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     2341 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     2401 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     2461 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     2521 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     2581 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     2641 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2701 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2761 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2821 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2881 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2941 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     3001 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     3061 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     3121 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     3181 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     3241 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     3301 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     3361 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     3421 ttttattgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     3481 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     3541 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     3601 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3661 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3721 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3781 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3841 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3901 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3961 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     4021 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     4081 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     4141 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     4201 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     4261 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     4321 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     4381 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     4441 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     4501 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     4561 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     4621 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4681 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4741 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4801 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4861 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4921 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4981 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     5041 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     5101 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     5161 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     5221 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     5281 tttctcattc ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa
     5341 ggaaacgcaa taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca
     5401 gtggcagttg ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca
     5461 agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta
     5521 ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct
     5581 gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg
     5641 ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt
     5701 ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga
     5761 atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat
     5821 gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta
     5881 aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg
     5941 ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc
     6001 tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc
     6061 cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg
     6121 tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct
     6181 gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa
     6241 cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata
     6301 cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt
     6361 caagatactc ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa
     6421 gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct tttactatca
     6481 agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt
     6541 caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca
     6601 taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc
     6661 gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc
     6721 cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac
     6781 taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt
     6841 caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag
     6901 tatttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc
     6961 tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt
     7021 atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat
     7081 tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc
     7141 tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag
     7201 tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc
     7261 gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt
     7321 ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta
     7381 ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct
     7441 gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca
     7501 gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat
     7561 tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct
     7621 tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag
     7681 caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt
     7741 cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg
     7801 ccgtgatatt cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag
     7861 cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca
     7921 gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga
     7981 tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac
     8041 aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc
     8101 catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt
     8161 acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat
     8221 tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg
     8281 ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg
     8341 tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga
     8401 aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc
     8461 accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat
     8521 tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa
     8581 caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa
     8641 gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta
     8701 cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca
     8761 attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc
     8821 aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact
     8881 gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca
     8941 gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc
     9001 ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat
     9061 tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt
     9121 ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag
     9181 taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg
     9241 aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa
     9301 atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc
     9361 atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct
     9421 caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg
     9481 agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa
     9541 agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt
     9601 tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac
     9661 atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa
     9721 atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag
     9781 taattggaac cctttaagta cgacgccata gtcataagac cattttcctt tataccactt
     9841 ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt
     9901 ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta
     9961 tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg
    10021 tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt
    10081 caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc
    10141 ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt
    10201 cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga
    10261 acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca
    10321 tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc
    10381 cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct
    10441 tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat
    10501 ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca
    10561 ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca
    10621 ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg
    10681 taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag
    10741 ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc
    10801 agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac
    10861 taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt
    10921 ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca tctagtccta
    10981 gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg
    11041 gtacgtcgta caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac
    11101 ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg
    11161 cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt
    11221 catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga
    11281 atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg
    11341 aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag
    11401 aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac
    11461 ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt
    11521 ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa
    11581 tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct
    11641 ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag
    11701 taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc
    11761 caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca
    11821 gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa
    11881 acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta
    11941 aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta
    12001 atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg
    12061 actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg
    12121 tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg
    12181 tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc
    12241 cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta
    12301 taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac
    12361 atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt
    12421 actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc
    12481 atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc
    12541 gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca
    12601 ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc
    12661 cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag
    12721 tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt
    12781 aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag
    12841 atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt
    12901 ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca
    12961 ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga
    13021 atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg
    13081 agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc
    13141 ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat
    13201 ctgaattacg tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt
    13261 tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt
    13321 ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga
    13381 tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta
    13441 tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct
    13501 aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc
    13561 ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga
    13621 cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg
    13681 tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct
    13741 ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt
    13801 gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc
    13861 ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa
    13921 ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga
    13981 ttttggtgta tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc
    14041 gcttttttat gctgttactt actggaacac ttatatgact gctattttgt atatcaacga
    14101 ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat
    14161 gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt
    14221 tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt
    14281 caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt
    14341 tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa
    14401 tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga
    14461 ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg
    14521 agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa
    14581 ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt
    14641 gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa
    14701 atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac
    14761 agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt
    14821 ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt
    14881 acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca
    14941 aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta
    15001 taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg
    15061 atatgcataa tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat
    15121 tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa
    15181 ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga atctactccc
    15241 aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc
    15301 aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact
    15361 tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta
    15421 ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga
    15481 ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca
    15541 ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt
    15601 acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct
    15661 ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga
    15721 ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc
    15781 tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca
    15841 ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga
    15901 tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt
    15961 gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt
    16021 tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga
    16081 cgggcaaatc tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa
    16141 gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca
    16201 acttctaatc tgtaaatcca attattataa tctgatttca gtactatata cgttttatct
    16261 ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt
    16321 tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa
    16381 agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca
    16441 attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct
    16501 ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt
    16561 tcaatttcct cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc
    16621 aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct
    16681 ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc
    16741 gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat
    16801 tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg
    16861 atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct
    16921 aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc
    16981 tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa
    17041 tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa
    17101 cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg
    17161 aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg
    17221 tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt
    17281 taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc
    17341 caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct
    17401 caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt
    17461 cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag
    17521 aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc
    17581 ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca
    17641 atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag
    17701 attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc
    17761 gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg
    17821 tcaatccagc ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg
    17881 aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag
    17941 cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc
    18001 tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac
    18061 gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag
    18121 gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga
    18181 tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag
    18241 aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt
    18301 aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca
    18361 gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca
    18421 aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt tacaaggact
    18481 cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa
    18541 tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat
    18601 cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt taacagatac
    18661 tcccccacag agcctcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa
    18721 caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa
    18781 agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt
    18841 agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg
    18901 cgaagatgca ggtgtttgca atcaacggca taactatgaa gatcaattga tggtcaaagc
    18961 agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt
    19021 caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa
    19081 ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt
    19141 ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt
    19201 caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga
    19261 ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt
    19321 aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga
    19381 acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg
    19441 aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg
    19501 gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga
    19561 tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca
    19621 aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc
    19681 tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc
    19741 caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc
    19801 aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct
    19861 atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg
    19921 taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc
    19981 attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga
    20041 ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga
    20101 tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt
    20161 taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt
    20221 tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg
    20281 agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga
    20341 aatttctagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag
    20401 agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg
    20461 tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg
    20521 gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag
    20581 aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact
    20641 caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt
    20701 acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt
    20761 taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa
    20821 aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct
    20881 ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt
    20941 ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag
    21001 gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag
    21061 aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa
    21121 atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca
    21181 gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga
    21241 ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg
    21301 tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg
    21361 gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa
    21421 aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa
    21481 aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat
    21541 tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt
    21601 ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg
    21661 tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc
    21721 agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg
    21781 atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca
    21841 agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt
    21901 ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga
    21961 gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg
    22021 tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct
    22081 gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac
    22141 cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg
    22201 tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta
    22261 tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt
    22321 aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt
    22381 tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct
    22441 gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt
    22501 catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag
    22561 ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa
    22621 cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag
    22681 acggaccaaa tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt
    22741 tatgaaagaa gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa
    22801 aaagatgaaa acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca
    22861 cccgagatcc agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag
    22921 gaaggaaggt tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa
    22981 tttggacata acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat
    23041 ttaaaaaatc tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta
    23101 ctgacatctg tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt
    23161 ggagactatc caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat
    23221 gtgttctcaa gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc
    23281 tacctgcatc ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc
    23341 cactttagtc cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa
    23401 tcccgtctgc ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt
    23461 aggcatccgt gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc
    23521 acagcttcac ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa
    23581 attaactgac atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa
    23641 tatcgaactt tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg
    23701 tgcacaagca ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa
    23761 gatgattgct acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga
    23821 agaagcttat cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt
    23881 tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact atcagttagc
    23941 taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa
    24001 tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa
    24061 tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat
    24121 ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa
    24181 atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga
    24241 agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac
    24301 gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt
    24361 atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc
    24421 catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt
    24481 cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa
    24541 atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag
    24601 ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag
    24661 aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt
    24721 tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag
    24781 gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg
    24841 ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc
    24901 aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc
    24961 gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct
    25021 atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta
    25081 gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc
    25141 gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg
    25201 tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta
    25261 ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac
    25321 atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac
    25381 cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc
    25441 catctggctc tggaaaatct acactcctta acttattgat tggatttatc cagccgactt
    25501 caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa
    25561 attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca
    25621 tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc
    25681 aactttcttc actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg
    25741 gcaacaagct gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc
    25801 agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa
    25861 aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc
    25921 ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg
    25981 aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg
    26041 aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac
    26101 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg
    26161 ttttttgaaa ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa
    26221 atggtgttca agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg
    26281 aacgtctctt tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc
    26341 gaactggtgt catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt
    26401 tttgcatcaa gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt
    26461 tcattgcatc ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt
    26521 gcatcgcttg gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt
    26581 acgcttcttt caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg
    26641 cgctatcgta gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg
    26701 taacatgggg ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct
    26761 ttagccgctt caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc
    26821 ccttcttttg cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca
    26881 ttagaatttc ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag
    26941 gcatctgcta cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat
    27001 ttgaagactt tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat
    27061 tcttcgtaac ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg
    27121 tcatttgctt ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta
    27181 taacctgcag gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg
    27241 gtcatttcat cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg
    27301 acgatcaccg ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga
    27361 ccatcattgg caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc
    27421 agtccaccaa gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct
    27481 tctgctttat catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa
    27541 ttcaaacttt gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa
    27601 ttatgcgcat cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca
    27661 gcttccagtg ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc
    27721 atctgtgtag caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct
    27781 tgtacaccac ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct
    27841 gaagcatttg ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa
    27901 tcctgatcta aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga
    27961 ccatactcgt taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca
    28021 gaatctgaat tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct
    28081 tgttcttgtt cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc
    28141 cgatcattcg cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt
    28201 cctgccaaag tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt
    28261 ctggaagcgt cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg
    28321 gtcaagattt cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc
    28381 gcttttcgat caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg
    28441 atcgtacttc ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg
    28501 gcaatccctt tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt
    28561 actgcatctt ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg
    28621 tacagtgtgc cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct
    28681 gattttaacg tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact
    28741 aagacacaga tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg
    28801 cgctttcgcc atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc
    28861 aaagcagctt ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta
    28921 tttgattcaa tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt
    28981 agttcaatat gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat
    29041 gtaagttttt tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa
    29101 ggagtacact tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc
    29161 gcgacttgct tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac
    29221 gtaaaaacgt ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg
    29281 accagatcac attgttattc gaagaaacag attataatta tcaagaagtt caattaggag
    29341 atgcgaagca catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg
    29401 caggaatgaa gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag
    29461 ctgcttatct cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa
    29521 caagcggtgc cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt
    29581 tggaacaaaa aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag
    29641 atatgaccat caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag
    29701 atgataaaaa tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac
    29761 aaacacaagt ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag
    29821 ctcataaggg acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac
    29881 tgatgctcca tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg
    29941 gtaaagcttt acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga
    30001 taatgataag actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc
    30061 tgctaacagc aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata
    30121 ttcacttact tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat
    30181 tttttgaagg agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc
    30241 taacgtcaac gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac
    30301 aggatgatct tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc
    30361 tgctgctaat ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc
    30421 tggatggcgt ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc
    30481 ttcattgatc acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt
    30541 ttttccttgt gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt
    30601 ccaagcagca ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca
    30661 cccttttttg ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat
    30721 cgggccgaat gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac
    30781 ctgatccaat gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc
    30841 accatctgaa accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg
    30901 ttttcctcct cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct
    30961 tgcttcgttg gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt
    31021 tccaattttt ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac
    31081 ttgctgcgtt tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt
    31141 tttgaaatca atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct
    31201 gcgtatgcat gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa
    31261 ttgaaccgat gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct
    31321 agttctccca cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat
    31381 tcattcaaac caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat
    31441 gcttgctgat gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc
    31501 ttcaccactt caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac
    31561 aagacacgta agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt
    31621 ttactatctt ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat
    31681 cgttctgctt ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg
    31741 tctgcagaaa cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta
    31801 tctactaata cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag
    31861 aaaggttgtt taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga
    31921 ttattcaact ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc
    31981 gtcaaatgta gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt
    32041 tcttctgctt tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg
    32101 ttttctaatg gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct
    32161 ttcggcaaat cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc
    32221 ccactttttg tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt
    32281 tgttcaggta ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca
    32341 ccagttttat ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat
    32401 ccggtcgtat cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca
    32461 acttcatctg ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa
    32521 ccgcaaatac tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga
    32581 tcatggggat agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt
    32641 tctagctgtc gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta
    32701 ttggctaaag tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct
    32761 aagtcttctg gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata
    32821 aactcgtaat ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt
    32881 accggttgat gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct
    32941 gcatccgcta atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca
    33001 ttcccgttgc tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct
    33061 ttccaccaca tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca
    33121 aagccaattg gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt
    33181 aattgaggcg tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg
    33241 gttccccatt tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag
    33301 ctgataagaa aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg
    33361 attgcttgtt ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata
    33421 atcgtttgcc catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt
    33481 agatcgtcta tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca
    33541 cgatcccagt ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac
    33601 ttttctactc taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa
    33661 gaaaaccatt ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg
    33721 tgtgttcctg cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct
    33781 gacgttgtca ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg
    33841 gaggatccaa ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac
    33901 aatgttcttc ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt
    33961 gcggcgatca gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct
    34021 acttcatatg cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc
    34081 ccctcttgga tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt
    34141 atcgtttgaa aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt
    34201 accagatatc cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct
    34261 gtaaaagctg tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg
    34321 tctcgtacaa atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag
    34381 atttttttga taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg
    34441 taaagaagat aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc
    34501 cagatccaat cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg
    34561 ttgaaggcat tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg
    34621 ctgatcatgt cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt
    34681 gcagtagagt ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg
    34741 tcattttgct ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt
    34801 gcccagtccg catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc
    34861 gattttggta tatctttata agccactaat aaaaccttct ttctatttga tatatcttta
    34921 aagattatac ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac
    34981 tttttcatcc attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa
    35041 tcaaccattg taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt
    35101 acctgtagac agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag
    35161 aatcacttcc aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg
    35221 cgggaaaggc agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa
    35281 aacacgacgg attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag
    35341 caagggattg aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga
    35401 ctttctacaa aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta
    35461 tgctcttgat acagaacaac atcttgactt actttttgag ctttcattga aagaattccc
    35521 tgtcgtcata ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa
    35581 tttccaaggc ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc
    35641 ttatttgttt ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct
    35701 tgaagcattg gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac
    35761 attagatgaa ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa
    35821 cgatgttgtc gctatccaga ccatgaatct cttgagacag catggttatc atatccctga
    35881 agatttttca attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact
    35941 gacaaccatc gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac
    36001 ttggttagag acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag
    36061 acaatcaaca aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag
    36121 cgaatcagcc aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga
    36181 tgaattattt tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg
    36241 accgcgtttt tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca
    36301 gtcatatgct gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc
    36361 aaagttctta ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat
    36421 tacgttatgg catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg
    36481 attacagcaa aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg
    36541 ttatagatcc ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg
    36601 atttttcttt ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg
    36661 tttccaacaa aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta
    36721 tccgatttgg gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac
    36781 aaaaccaaag caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg
    36841 atcgtataca gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat
    36901 atcgggtgtt tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc
    36961 actatgatac gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt
    37021 gggatacgta taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag
    37081 aaatgctgga aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt
    37141 ctcctgatga acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg
    37201 ctgtaaaaga tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg
    37261 ctacggttca aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat
    37321 atggctatgt tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata
    37381 gcgatttttg tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat
    37441 atcagcagca atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt
    37501 caaaagataa agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt
    37561 atgcagaagg aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga
    37621 tcggaaaata tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc
    37681 ccccaacgtt tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg
    37741 cgatagaatt tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt
    37801 tcagttatat aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga
    37861 ccttcgacga ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt
    37921 ggtatatctt caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta
    37981 tcggtatgcc attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga
    38041 ttgaagcaac gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca
    38101 aagaatatac gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt
    38161 accaattagg gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct
    38221 tatcaaacta taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    38281 agccaagcaa aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca
    38341 agcttttgaa caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga
    38401 gagacaaatg gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc
    38461 agctgattga acaccgttta gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta
    38521 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
    38581 aactggcttt tgaactccgt ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa
    38641 taagaacagc ccaaaattag atttctagac aattttttat aagttatttc attacattgg
    38701 ctttttcttt tatgattctc cactgttttc tcggtactct ttaggggatc tgccgacgat
    38761 tttcttgaat aatttagaga aatacgtggt gttgttgtag ccaatctcac ttgctatctc
    38821 attgatgttt tgattactgt gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt
    38881 caaataagct gaaaaacttt ttttcgtttc ttttttgaac agctgtccta gatacatgac
    38941 atttaaatgc aggttttctg acacttcttt gagtgtaagt ggttcttgat aatgctggtg
    39001 caggattacg attacttttt ccacattgtc actgtacttt ttctgtctct gatgcttttt
    39061 gatgaatgac agaagtagct gctgaagttc ttgcaccgtt tttgcctgat ggattttttc
    39121 gattccttgt aaatattcct catcttccag atcaatcaat tcccgttgaa tctccataaa
    39181 cagtaaaaaa ctggaatatc gtatatcttc cggcatcatg gctgctaact gaaactgttc
    39241 aaaaaaatca gtgatcattt tttgagcgac ttccaattgt ttattttgca gcacgcgtct
    39301 gaaacttgaa aaatcgatgg tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg
    39361 ctcgtcttta tctccataaa attgatgcaa ctgtagattg tctttcgctt gttggaatgt
    39421 ttcagggatt ttgtccaaag aatctgtctc ttccccgata ctgatcagcc attcctgatc
    39481 aggaaagttt tcttgaacaa aacaacagaa gccatcaact tcatctgcct ccaacaacaa
    39541 cgtgatcaat acacggtctc cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg
    39601 caaccatcta ttgataagag tatcatgagt tcttgccagt tgaatcaata ctatcctatg
    39661 ctgcttctca cttatttgtc ctattatttc ttcttcactt atttcatcca attcttcatt
    39721 caaccattga ctgaataaca gttcttgata gatttcttgt tgattttttt gttcattttg
    39781 ctgatctaac cgtgttttga ttttcttgag gctttcaacc aattcgaact tattcacggg
    39841 tttcatcaaa taattcacgg cgcctaattg catacctcct ttcaaataat cgaattcttg
    39901 gtatcctgat aaaatcataa attcaaaatt atgacgttct ttttgagccg cttcaataaa
    39961 ttccagtcca tttatttctg gcattgtcac atccgttact actaaatcaa tctgtcgctg
    40021 ttcaagtaca gctagtcctt ctttggcatt ctctgctgtt gctactattt gaaagcctaa
    40081 actttgccaa tcaattatct tttttaaccc attcaaaatc atgtattcat catctaccaa
    40141 taacacttga tacacgctca tccctccttg aatgtaatag aaacggtcag tccacctgtc
    40201 tcattattct caagcgacat ataataactg ctgccgaagc tggcacgtaa acgttcattc
    40261 acgttttgca gaccgataga accgtgaagt tccactttag gatgagacag tttttcttca
    40321 atctgtttta gccgtttttc agtcatccct tttccattat ctttgatgat gatccttacc
    40381 cgtttgcctt cctgcaagac tttgacactt agcgcattat cgaatcgagt aaaatcaata
    40441 ccgtgtttaa aataattttc aactaatggc tggatcacaa atttaggtac ttcgatcttt
    40501 tccaaatcag gatcgatcat aaaatgatag gctactcgat taggataacg catttgatac
    40561 aaatagacat atttttcgca aaagtccagt tcttctttta aagtgatcgt tttctcttga
    40621 ttcgtattgt ttcgaagcaa agcggaaaac gcatacacaa catctgctaa ttcttcactt
    40681 ccttcgctga ttgcatacat tcggatatat tctaatgtat tatataaaaa gtgtggattg
    40741 atctgtgctt gaagcgcccg catatgtgcg tcttgctgtt tgatttctaa tttgtagatg
    40801 tcttctacaa actgattgat gctatccaac atttcgttga taccgattga aagttccttc
    40861 agttcatatt ccgtgtttgt tatgtcgatc cgcgtctcta aattccctgc tgctgtttga
    40921 tccatggcct gcatgacgat agatacttgc tgtgaatatc gtttgaatgt tttataaaga
    40981 aagataagca gtatattgtc taaaataatg ataccaatga tcagcggggc aagatttttt
    41041 cccgtttcga tttttacact ttttttatct agtacagcta atatacgata atcatcagac
    41101 agttcttggt gttgaacaaa attgtttttt tccagatcgt caatcggcaa ttgtgaattt
    41161 ttcttcagac tctctgtgat gatcatttct tgtctttcaa acgtttcttt agctatttta
    41221 ttatcatgga atgtgtatag tcgatttgtc gttccagaaa tcatgtataa agagagtcca
    41281 ttgaatgacg ttagattttc taaaatcata tctagttctt tcttttgaaa accaataaaa
    41341 atacttcctg actcagctcc tccttgtgtg ataggcaact tgatataaaa ggcgtctagt
    41401 aattttggtg tccccttttt tatttcccca cctttgtttt ctctagaaga ttcataatac
    41461 tccggtagtt gattcaacac gatatatatc gcacttagtt cttcataatt tgcgtagaga
    41521 tctttgattt gatttgggaa agaaactaat tcatcggtac tgttttgctg ttcgtataca
    41581 tacatcagat actgatctat gggttttgtt aagtaggtcg tcacattttc gatcttttcg
    41641 gaattcccta caagctggtt tttgatgatc ctactcaagg ttgttttatc attcagcatt
    41701 ctgcttgtgc tttgaacggc atcacttgtc atgttttctg cctgtttttg attttgttcc
    41761 caagtatgga tactaaaaat aacggtagca gtcatcgtta caccgatcat gataaccgcg
    41821 tatttccgca acagacgatt tagtaaggtt ccttttgaaa atagaatccg aaacttattc
    41881 ataactttcc aactgtttat ctaattcttc acgccaatgt gttgtcatcg taccattcca
    41941 aaccgtcaaa agaccaatga caccaaacag gatcaatcct ttgaattgcc aagtcacgcc
    42001 taaaattata ccgctaccaa taattatttt tagaaaagac gaaaagctgg aaaaaaccga
    42061 aataaaactt agctttagca gattaggaac agagatcgta tattcactat ctaaaatcat
    42121 actgtattga taagtagcaa ataggcagag caaggcaaaa agtaaaataa aatcaatagc
    42181 taaaaaaatg agccctttga tttgaacaga taaatataaa ttgtaactca ataaagcagt
    42241 accagataaa aataaactaa atagcagatt gcctcttaaa aaatttcttt tatatatatt
    42301 ccagcctctt tttattgtga tttctttata ctcgaatccg tacaaataaa atagttcatt
    42361 gactgttttc catgccgcac ctaatccaaa gatgatcccg ccggcaaatg tatatatcca
    42421 gaaatacaag gttagtttta taatgaccca gatccggata aataactctt ccaaaacctt
    42481 ccctaacata tcttctcctc caaacattaa gtttatactt tctcattata caaaaaaagg
    42541 ttgggcaatc agcaccttga cctgattatt agagaaacaa acggaaaaat agttcctcat
    42601 attttgtcgt tttgtgagcg aataccgagg gttagctgat tgaacaccgt ttaccaagag
    42661 tttgtgaaat agccgcttag tttcgagtag taagaaaaat tttgtgaata tggctttcca
    42721 catttttaca tatatttagc ttaataccga gaaactggct tttgaacacc gtttattcgg
    42781 aacaagaggt tgtgacacgt cttttgtcac aacccctttg tctttattca ttattattct
    42841 tgtcggaact catcatattg tttttgcatt tctttcaata ccttatcata gccagccttg
    42901 tccaaagcgt ctttcaattt cggtaatgtt tcatctggat caacagtccc tgtattcaat
    42961 ccatctaaat attgattcat gacattagaa atattactta attctgtttt gacactgtca
    43021 gtgttgaagc tgaatcctaa aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt
    43081 ttggcaatca tgtcatccgt aatagactct tgtgtatata gaatcttgtt gtttcctgta
    43141 ttccaagcag acatatgtgt atttggctga taaccatcta gcaatttgat tttatcatct
    43201 gaaccatcga ctttttccca tgcttctccc tcgatccccc aaacaagccc attcaataat
    43261 tctggatcac tgttaagcag ccctagaagt tctactgatt tttctttatt ttttgaagta
    43321 tttgatacga caaaatttgc catttgtgct tgagctgtag attttagtgg aacagtaatt
    43381 gcttttgaga caagttcttg ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac
    43441 ggtccttgtg tttctccacg cattagccaa gtatttcctt ctagtggata atccttattg
    43501 ctcgttgcag cgtcacttgg aatgagtcct tgtttgtacc agctatgcat tgtacgtaac
    43561 agtccgatat acgtttcatt atcgtattgg ttgatgattt tcgaatcatc tccatccaga
    43621 tcaatagcaa atggtaacgt attccctaaa ggataatcaa agttcccttg gactttgaag
    43681 ccttgtccaa tggcaaaagc tgccgtgttt ggttcttttt catggaattc cttcaagact
    43741 ttttctgcat cagcgtaaga ttggattcca tcaattgaaa gatcgtattt atctagtaaa
    43801 gctttgttaa aagtaagtac ttgttgagca aagacatttc cattgactgg gaaagcatat
    43861 aattttccat tgaccagatt tcctttaata tatgcttcat ctaaatcatt ataagctttt
    43921 tcagcgtact taggcgctaa ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa
    43981 acatagttgt tcgcaaaggc gatatcataa ttttccccgg aagaaatgat gacgttcatt
    44041 ttcttttcgt aatcgcccca gccaatgtaa ttgatattga cttttacacc gatctctttt
    44101 tcgattcgtt tgtttgctac ttccatcaat tgatcgaaat tatctggttt atctccgatt
    44161 tgatacatct gcaaagtgtc cgaattgcta gcttctgcct ggtctttaga accagaacct
    44221 ccgcatgctg ctaatgttcc caacaacaac gctgaaacac ctgtaaaggc tagtttcttc
    44281 cataacttca tttttcttcc tcctaatttt atgatttata tttttgtttc tttattcttt
    44341 tactccgccg attgttagac ctttgacaaa atgcttctgg aaaaatggat agctgatcgc
    44401 aataggcaac gtcgagatga ctacgatcgc cattctggca gattctcctg gaactgaggc
    44461 taatccgcct gtcaattggc ttcctgctcc tgaattttgt gtcagatatt gaatattact
    44521 ttggattttc atcaaaagat actgtagtgg aactagctta tcatcttgga tgtatagtaa
    44581 agcattgaac cagtcattcc agtaacccaa tgcggcaaat aaactgattg tcgcaatacc
    44641 tggaacagct aacggtaaaa cgatctgaac aaatattcgc agttcgctag ctccatcgat
    44701 ccgtgccgat tcaataatcg aatcagagac cgaacgttta aagaatgtgc gcatcacaat
    44761 aatattgaag ggactgacag ccattggcaa gatcaatgcc caaatcgtat cttttaactg
    44821 aagaagattt gtcataacaa gataattggc caccatccct ggtgagaaca acatcgtaat
    44881 caaacaaaaa acggtaaaaa aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc
    44941 atataaagaa gtagccgtgc tattgatcag tgtacctaaa acagtaacaa gtacagtgac
    45001 gaaaagcgct tggaaaattt tatcctgcat ctggtcgaac aagtacgtat aaccaaatat
    45061 actgaacttt tctggccaaa aactgtaccc gtgagtcgac aatatggact catcggtaaa
    45121 agagatggcg ataacaaata taaatggcaa aatacaggaa atagcaaaga gtgcaatcat
    45181 tatattagag aaaaaattag ccgtcttatt gaaagagcgg acttggactc tctccacttt
    45241 cttttttttc atttttttcc tccttagaat aatgcagaat cgctatcaaa acgtcgggcg
    45301 atcgtatttg tgatcatcaa caagacaaag ccgacaactg attggtaaag tcctgcagca
    45361 gccgtcatac caaaatctcc tgtggatgtc aggccattgt agatatacgt atccaaaact
    45421 tgagtcacat catataatgc accggaattc ctaggaatgt tataaaacag gccaaaatca
    45481 gctcggaaaa tgtttcctac ggctaaaatc gttaaaatcg tcattagtgg aataagttgt
    45541 ggtatcgtca catgtttgat ctgctgccat ttgctagctc catcaaccat agcagcttca
    45601 taataagtcg gatcgattcc cataacagat gcaaagtaaa taatgctgtt ataaccaatc
    45661 cctttccatg taccaatgaa caccaagata aacggccaat atttcggttc attataccaa
    45721 ttgacagctt ctcctccatg tccagtgatc cactgattga atattccttt gtcaggactt
    45781 aaaaaagcgt aaacaaagta actgataatg acccaagata gaaaataagg tagtaaggac
    45841 atcgtttgat aaactttaac gagtcgttta tttctcaact cgctcataat gattgcaaat
    45901 acgactgaga tcagcagatt caaaaggata aatgctacat tatataaaat tgtattttta
    45961 gtgatgagaa atgcttgatc cgaagaaaat aaaaatttga aattttccca acccacccaa
    46021 ggactttccc tcaagctagc caagaaccca tctgctgaaa tatgaaaatt tttgaatgct
    46081 acgacattcg ccattacagg tatgtaaaag aaaaaaatca tccaaagcat acccggtaca
    46141 gccatcagga caagtgcttt ataacgccat agattcataa aaaaatgatt gaaccccttt
    46201 cgttttttca tctaaaaacc cctttcgatt cattcgcaac gtaattatag cgctttcaat
    46261 aatgagaata aataaacaaa gtatagggaa aatgaaacaa agtataggag aggttgtgaa
    46321 atcagcgttt tggcctgagt attagagaaa aaaacgggaa aatagttcct catattttgc
    46381 cgttttttga gctaataccg agggtcagct gattgaacac cgtttatcag aggttgtgaa
    46441 ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac tctgagtcac
    46501 aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg aacaaacggt
    46561 gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc atccgaaagt
    46621 cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt cttttagcta
    46681 tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg gtcgatattg
    46741 ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt ttaaatataa
    46801 gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc cttcatgcaa
    46861 aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt ggagttttgc
    46921 cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac ggatatcttc
    46981 ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt ttttaggtga
    47041 tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt
    47101 atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca ataaatcgat
    47161 tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt tatgcaaaat
    47221 gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg catcttcgaa
    47281 ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga tcgttccttt
    47341 tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta aaaacttatc
    47401 taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct gattcactta
    47461 ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc tttctgccaa
    47521 gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta ctacaagaat
    47581 caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt
    47641 gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg tcctgcgcca
    47701 gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca aggtacaaga
    47761 cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata aacgaatggc
    47821 tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa ttgccgcctt
    47881 gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta ggaagagtcc
    47941 tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat aaaataatgt
    48001 atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa
    48061 caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca acgtaacagc
    48121 ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa taactaccat
    48181 cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact gtgcattgga
    48241 atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta atttgaagat
    48301 cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat ctaacaacaa
    48361 ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg atgtatacgc
    48421 ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac cgataaatgc
    48481 cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt tcatgatgat
    48541 gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct gcgacagcgc
    48601 ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca tacaaggaaa
    48661 aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc cgccaccaga
    48721 agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc
    48781 gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt cattcaatga
    48841 attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag agatcagtag
    48901 aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct tagcattttc
    48961 gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg aaatgatatt
    49021 actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta attgatctaa
    49081 tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa cattactatc
    49141 attttaattg gtaaaacaga aactggcaat cgattttata taacttttat aagaacaatt
    49201 gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag ccttttatgg
    49261 ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg ttatattttt
    49321 aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg atatagcttc
    49381 cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc aacgctcagt
    49441 tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat taggaaaatc
    49501 agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag agattgttcc
    49561 aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc ttttgttcag
    49621 tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa cgataccgcc
    49681 gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac ttaccaatag
    49741 tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat tcgttctcct
    49801 atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag
    49861 aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt aaaaagagtg
    49921 tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc ttctctttag
    49981 aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt tcaacaagag
    50041 gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat gattcaagaa
    50101 aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa gaatatttgg
    50161 attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac gaaaattcag
    50221 cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct atggcttttt
    50281 acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa
    50341 ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa acagcattgc
    50401 tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat ttgacaacgc
    50461 ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt atgactcatc
    50521 tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc ttgactttag
    50581 atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa catccgacat
    50641 ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa
    50701 atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc cagattccaa
    50761 cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca gataaaggta
    50821 ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat actgcttatg
    50881 atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag
    50941 cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct cgtgtaccag
    51001 gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac tatgcacata
    51061 attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg
    51121 taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat gattttggaa
    51181 aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca gcatttgaag
    51241 atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat ttgatcgttc
    51301 attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc gctccgcaag
    51361 attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg
    51421 aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa tggtatttat
    51481 atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt cacagttcct
    51541 tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt tctctttctt
    51601 catttaataa tttattatca tagaatttga taaatgggaa ccagataatg aatgcgatga
    51661 ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca acaaatgctc
    51721 cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg atttccagcc
    51781 gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac ggaaccgcta
    51841 aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata ttgaataatc
    51901 ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg aggaatgcaa
    51961 taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa gaattgttga
    52021 attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta gctaatacga
    52081 tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc cacaatgcat
    52141 ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc
    52201 caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat aatccattga
    52261 tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa
    52321 ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca cacatacaat
    52381 acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa gttcctaaac
    52441 gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca ttgatagaag
    52501 caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata aagaatgcaa
    52561 aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca tctcgttcaa
    52621 ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc cccatcgtca
    52681 ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt
    52741 ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg
    52801 tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta agttgcgcaa
    52861 ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt tattctcctt
    52921 tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt cttggttgag
    52981 tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc ttacaacaag
    53041 aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca agaatgtaaa
    53101 tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga acaaaaaata
    53161 ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt gttctcctac
    53221 tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac ttttagctga
    53281 tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac
    53341 gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt ctaattcgat
    53401 tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt tttgatacaa
    53461 ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc
    53521 atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt tcagaagttg
    53581 agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact tctcttctac
    53641 ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt
    53701 tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt ttattttttc
    53761 agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga aattctcaaa
    53821 aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc taacgattgc
    53881 tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta aaaaaacaaa
    53941 atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa cggctacctt
    54001 tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac tgaatatgat
    54061 ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat atccttcttc
    54121 ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt tcggttcgta
    54181 cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct ttttttgctg
    54241 tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt ctccaactac
    54301 ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta aaactggaat
    54361 tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc cttttaaagt
    54421 tgtttcattg atctcataat agccttttac acttaccact gtaatatttt taccgaattc
    54481 actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac agataatcaa
    54541 tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat gcaaagccaa
    54601 ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt tgctgaaatg
    54661 cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag tcaatgggtt
    54721 ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat cgagtagtcc
    54781 attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca atcttttcag
    54841 aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt gattcgactt
    54901 agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata cacctgcgat
    54961 tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct tagaaacttc
    55021 ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga tccccaaagc
    55081 caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag cttctcttgt
    55141 ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa aacggctttg
    55201 ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat ccatgataaa
    55261 atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct tagaaacgat
    55321 ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg tcaaactgga
    55381 acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat cttccaaaag
    55441 caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt gcggttctct
    55501 agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca gtctttgtaa
    55561 aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt ttttttctac
    55621 taactctttg atccgctgca agtaattacg gattgtccga tcagataata acaattcaga
    55681 agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc tcaacagcaa
    55741 tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc ttcgatcacc
    55801 tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg gatactaaca
    55861 acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc
    55921 gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc ttctgtagct
    55981 gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt tgtctttgag
    56041 gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt cataaatcaa
    56101 tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat tcaaaatata
    56161 tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat ttctggcacc
    56221 gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca cagtgaatat
    56281 tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa
    56341 cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt actttccact
    56401 agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag caatcgaaaa
    56461 gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa
    56521 aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa tgatagcaat
    56581 ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca tctaatgatt
    56641 caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa tatacatgat
    56701 tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc ctggaacccc
    56761 gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt tatatctata
    56821 ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa aaggaataaa
    56881 ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc ggtctactgc
    56941 tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag aaaatgcaaa
    57001 ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca aaagactaat
    57061 gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat
    57121 ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa ggagaactga
    57181 aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat tcttgttcaa
    57241 cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca aagaagcatt
    57301 ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa taaaatgaat
    57361 caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta atagtgagaa
    57421 catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt caacaaaacg
    57481 aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg attcaaataa
    57541 tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg atcgtaaaaa
    57601 caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta ttttttgttt
    57661 taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc gtcctttctt
    57721 tttactttta ttttttacag tcatctatga caggttgtaa atactatgga acatcatatg
    57781 gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa aaacatatcg
    57841 ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata tgctttatga
    57901 atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt acagaggtct
    57961 cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc
    58021 catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa ttaatggtgg
    58081 tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat atgccaatgg
    58141 ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat tggaacgctc
    58201 aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat aaaatcactt
    58261 ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag gtatctttga
    58321 caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct tccaaaagaa
    58381 ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg aggataaact
    58441 gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt acctacacaa
    58501 aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga aatagaattc
    58561 ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct accaaatgcg
    58621 aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc gtaaccttta
    58681 ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc tccttattga
    58741 caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt tgtagaaaga
    58801 aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc gaaactcaag
    58861 aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac aaaggtttat
    58921 ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg ggttcaacct
    58981 tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta gtttccattc
    59041 aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta ttttagctca
    59101 tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac ccaatattga
    59161 caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat ttccactgct
    59221 tgttggattg tttcctctta tatctgagct atcagcaact tttctattct cttttgttaa
    59281 aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg catccaattc
    59341 ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta agccatctat
    59401 cgccagtttt ccatctgctc ctgtggttag acctgactgc agaacggttc cattggcatc
    59461 ttgtaactca aaaacagctc cttgcaggac ttctccgctt tggtcatcga tctttgtcaa
    59521 cacgacaccg cctggtgtaa gtcgattttc tttcgtcaat tctaccaccg ctgtctgact
    59581 tctttcgatc tcaaactcga ttggtgtcgc atccaattca taccccgttg gcgcttgggt
    59641 ttctaccagc tgatacactc ctggtgctaa gccgtcaatc gccagttttc catctgctcc
    59701 tgtggttaga cctgactgca gaacggttcc attggcatct tgtaactcaa aaacagctcc
    59761 ttgcaggact tccccgcttt ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag
    59821 tcggtttgct ttcgtcaatt ctaccaccgc tgtctgactt ctttcgatct caaactcgat
    59881 cggtgtcgca tctaattcat accccgttgg cgcttgagtt tctaccagct gatatg
//
LOCUS       DAPBBG010000012        58772 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPBBG010000012 DAPBBG010000000
VERSION     DAPBBG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 58772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.012"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            212..604
                     /locus_tag="RD477_000847"
     CDS             212..604
                     /locus_tag="RD477_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7633227.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="RD477_000848"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="RD477_000848"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDT7633228.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="RD477_000849"
     CDS             3165..4775
                     /locus_tag="RD477_000849"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDT7633229.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="RD477_000850"
     CDS             5067..5936
                     /locus_tag="RD477_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDT7633230.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="RD477_000851"
     CDS             6101..7372
                     /locus_tag="RD477_000851"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDT7633231.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="RD477_000852"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="RD477_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDT7633232.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="RD477_000853"
     CDS             8763..9026
                     /locus_tag="RD477_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDT7633233.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="RD477_000854"
     CDS             9471..9920
                     /locus_tag="RD477_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7633234.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="RD477_000855"
     CDS             9977..11395
                     /locus_tag="RD477_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7633235.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="RD477_000856"
     CDS             11395..12255
                     /locus_tag="RD477_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDT7633236.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="RD477_000857"
     CDS             12429..13202
                     /locus_tag="RD477_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7633237.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="RD477_000858"
     CDS             13349..13714
                     /locus_tag="RD477_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDT7633238.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="RD477_000859"
     CDS             complement(13862..15070)
                     /locus_tag="RD477_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7633239.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="RD477_000860"
     CDS             complement(15089..15367)
                     /locus_tag="RD477_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633240.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="RD477_000861"
     CDS             15521..16216
                     /locus_tag="RD477_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7633241.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="RD477_000862"
     CDS             complement(16658..17455)
                     /locus_tag="RD477_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDT7633242.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="RD477_000863"
     CDS             complement(17452..18636)
                     /locus_tag="RD477_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDT7633243.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="RD477_000864"
     CDS             complement(18661..19908)
                     /locus_tag="RD477_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDT7633244.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="RD477_000865"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="RD477_000865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDT7633245.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="RD477_000866"
     CDS             21888..22202
                     /locus_tag="RD477_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDT7633246.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="RD477_000867"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="RD477_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDT7633247.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="RD477_000868"
     CDS             22887..23531
                     /locus_tag="RD477_000868"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDT7633248.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="RD477_000869"
     CDS             23546..23875
                     /locus_tag="RD477_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDT7633249.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="RD477_000870"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="RD477_000870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDT7633250.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="RD477_000871"
     CDS             24863..25687
                     /locus_tag="RD477_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDT7633251.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="RD477_000872"
     CDS             25680..26027
                     /locus_tag="RD477_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDT7633252.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="RD477_000873"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="RD477_000873"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDT7633253.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="RD477_000874"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="RD477_000874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDT7633254.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD477_000875"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD477_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDT7633255.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD477_000876"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD477_000876"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDT7633256.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="RD477_000877"
     CDS             31657..32451
                     /locus_tag="RD477_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633257.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="RD477_000878"
     CDS             32441..34483
                     /locus_tag="RD477_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633258.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34755..34904
                     /locus_tag="RD477_000879"
     CDS             34755..34904
                     /locus_tag="RD477_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDT7633259.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34915..36429
                     /gene="dltA"
                     /locus_tag="RD477_000880"
     CDS             34915..36429
                     /gene="dltA"
                     /locus_tag="RD477_000880"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDT7633260.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36426..37631
                     /gene="dltB"
                     /locus_tag="RD477_000881"
     CDS             36426..37631
                     /gene="dltB"
                     /locus_tag="RD477_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDT7633261.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37676..37909
                     /gene="dltC"
                     /locus_tag="RD477_000882"
     CDS             37676..37909
                     /gene="dltC"
                     /locus_tag="RD477_000882"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDT7633262.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37912..39180
                     /gene="dltD"
                     /locus_tag="RD477_000883"
     CDS             37912..39180
                     /gene="dltD"
                     /locus_tag="RD477_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDT7633263.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39321..40403
                     /locus_tag="RD477_000884"
     CDS             39321..40403
                     /locus_tag="RD477_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDT7633264.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40403..41206
                     /locus_tag="RD477_000885"
     CDS             40403..41206
                     /locus_tag="RD477_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDT7633265.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41276..42016
                     /locus_tag="RD477_000886"
     CDS             41276..42016
                     /locus_tag="RD477_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7633266.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42027..43031
                     /locus_tag="RD477_000887"
     CDS             42027..43031
                     /locus_tag="RD477_000887"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDT7633267.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43076..44920)
                     /locus_tag="RD477_000888"
     CDS             complement(43076..44920)
                     /locus_tag="RD477_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633268.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45149..45649
                     /locus_tag="RD477_000889"
     CDS             45149..45649
                     /locus_tag="RD477_000889"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7633269.1"
                     /translation="MTFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQ
                     GNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPG
                     VVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRR
                     RTQKFL"
     gene            45777..46460
                     /locus_tag="RD477_000890"
     CDS             45777..46460
                     /locus_tag="RD477_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDT7633270.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46769..46842
                     /locus_tag="RD477_000891"
     tRNA            46769..46842
                     /locus_tag="RD477_000891"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46803..46805,aa:Arg,seq:tct)
     gene            47001..47360
                     /locus_tag="RD477_000892"
     CDS             47001..47360
                     /locus_tag="RD477_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDT7633271.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47483..49114
                     /locus_tag="RD477_000893"
     CDS             47483..49114
                     /locus_tag="RD477_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDT7633272.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49176..49817)
                     /locus_tag="RD477_000894"
     CDS             complement(49176..49817)
                     /locus_tag="RD477_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDT7633273.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50003..50079
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50099..50407
                     /gene="rplU"
                     /locus_tag="RD477_000895"
     CDS             50099..50407
                     /gene="rplU"
                     /locus_tag="RD477_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDT7633274.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50434..50778
                     /locus_tag="RD477_000896"
     CDS             50434..50778
                     /locus_tag="RD477_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDT7633275.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50791..51084
                     /gene="rpmA"
                     /locus_tag="RD477_000897"
     CDS             50791..51084
                     /gene="rpmA"
                     /locus_tag="RD477_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDT7633276.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51518..54325
                     /locus_tag="RD477_000898"
     CDS             51518..54325
                     /locus_tag="RD477_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7633277.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54516..55007
                     /locus_tag="RD477_000899"
     CDS             54516..55007
                     /locus_tag="RD477_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7633278.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55110..56099
                     /gene="manX"
                     /locus_tag="RD477_000900"
     CDS             55110..56099
                     /gene="manX"
                     /locus_tag="RD477_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDT7633279.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56132..56935
                     /locus_tag="RD477_000901"
     CDS             56132..56935
                     /locus_tag="RD477_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDT7633280.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56956..57870
                     /locus_tag="RD477_000902"
     CDS             56956..57870
                     /locus_tag="RD477_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDT7633281.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57953..58327
                     /locus_tag="RD477_000903"
     CDS             57953..58327
                     /locus_tag="RD477_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDT7633282.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58466..58711
                     /locus_tag="RD477_000904"
     CDS             58466..58711
                     /locus_tag="RD477_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7633283.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT        18878 a        10124 c        12345 g        17425 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtgcga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg
    34621 atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc
    34681 tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat
    34741 gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat
    34801 tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact
    34861 ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa
    34921 atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct
    34981 tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct
    35041 tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag
    35101 tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt
    35161 gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg
    35221 gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa
    35281 gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt
    35341 acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa
    35401 atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag
    35461 cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt
    35521 tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac
    35581 tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca
    35641 tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg
    35701 gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat
    35761 cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt
    35821 tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc gattggctac
    35881 gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa
    35941 acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa
    36001 aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca
    36061 ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa
    36121 ttacatggat accggattga actcggggat atcgaacatt atctgctcca agacaaccga
    36181 atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct
    36241 tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa
    36301 gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat
    36361 ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat
    36421 cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag
    36481 ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt
    36541 atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac
    36601 agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg
    36661 tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa
    36721 ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg
    36781 gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag
    36841 atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa
    36901 caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc
    36961 ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt
    37021 ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac
    37081 gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca
    37141 gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca
    37201 tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag
    37261 aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc
    37321 gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata
    37381 tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca
    37441 tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta
    37501 agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt
    37561 tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt
    37621 tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga
    37681 agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg
    37741 tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt
    37801 cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg
    37861 gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag
    37921 aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt
    37981 acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc
    38041 atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa
    38101 tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt
    38161 ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa
    38221 tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc
    38281 gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat
    38341 acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct
    38401 gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg
    38461 ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat
    38521 cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg
    38581 ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa
    38641 gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca
    38701 ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc agagctcaaa
    38761 cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc
    38821 ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag
    38881 aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc
    38941 aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt
    39001 accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat
    39061 caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac
    39121 gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa
    39181 agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga
    39241 taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat
    39301 atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt
    39361 ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca
    39421 aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg
    39481 ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa
    39541 caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag
    39601 tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga
    39661 ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa
    39721 aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac
    39781 gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt
    39841 tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga
    39901 atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag
    39961 taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt
    40021 tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt
    40081 taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat
    40141 tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt
    40201 ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag
    40261 ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt
    40321 atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg
    40381 aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa
    40441 agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat
    40501 aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag
    40561 catgcagcag cacttattga gttttctagt cgataattta cttttattcg cattcaacta
    40621 tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt
    40681 attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc
    40741 agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt
    40801 gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga
    40861 agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact
    40921 catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc
    40981 tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg
    41041 tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt
    41101 gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga
    41161 acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt
    41221 attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa
    41281 attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta
    41341 tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa
    41401 attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca
    41461 tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa
    41521 tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt
    41581 gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg
    41641 tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat
    41701 cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag
    41761 tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg
    41821 aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc
    41881 taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa
    41941 aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa
    42001 agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa
    42061 gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt
    42121 tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag
    42181 aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag
    42241 gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg
    42301 gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat
    42361 tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat
    42421 tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt
    42481 tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat
    42541 caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag
    42601 aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg
    42661 tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca
    42721 atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt
    42781 tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct
    42841 cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct
    42901 ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag
    42961 tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct
    43021 tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata
    43081 caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt
    43141 cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac
    43201 gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg
    43261 cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt
    43321 ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga
    43381 tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga
    43441 aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg
    43501 tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct
    43561 tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca
    43621 tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct
    43681 cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt
    43741 tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa
    43801 tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt
    43861 aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac
    43921 ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac
    43981 tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa
    44041 gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat
    44101 agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata
    44161 aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg
    44221 atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa
    44281 gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa
    44341 cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg
    44401 gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg
    44461 agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg
    44521 agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta
    44581 gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt
    44641 tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag
    44701 tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt
    44761 ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa
    44821 ttcctgttaa aaataacaat gataatataa tgattatgct ccactttata cgtaaataaa
    44881 aagattcttt tttattatat atgttattaa aaaaagacat aaaggtattc ctttctaaaa
    44941 cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa ttatttataa taaaaaatga
    45001 gataaagatg aggaaataga attagaaaca tataattatt aaagggaaac atttttacag
    45061 ctataatttg aaaaaaattt atcgttatta tatgattaag aaggattgaa taaagaaaga
    45121 agttgaaaac gatacctgat tacaaaaaat aacttttata agttctataa cgttgattat
    45181 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
    45241 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
    45301 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
    45361 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
    45421 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
    45481 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
    45541 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
    45601 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
    45661 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
    45721 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
    45781 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
    45841 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
    45901 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
    45961 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
    46021 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
    46081 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
    46141 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
    46201 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
    46261 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
    46321 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
    46381 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
    46441 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
    46501 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
    46561 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
    46621 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
    46681 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
    46741 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
    46801 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
    46861 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
    46921 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
    46981 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
    47041 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
    47101 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
    47161 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
    47221 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
    47281 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
    47341 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
    47401 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
    47461 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
    47521 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
    47581 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
    47641 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
    47701 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
    47761 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
    47821 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
    47881 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
    47941 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
    48001 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
    48061 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
    48121 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
    48181 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
    48241 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
    48301 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
    48361 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
    48421 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
    48481 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
    48541 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
    48601 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
    48661 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
    48721 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
    48781 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
    48841 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
    48901 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
    48961 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
    49021 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
    49081 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
    49141 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
    49201 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
    49261 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
    49321 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
    49381 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg
    49441 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
    49501 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
    49561 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
    49621 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
    49681 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
    49741 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
    49801 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
    49861 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
    49921 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
    49981 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
    50041 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
    50101 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
    50161 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
    50221 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
    50281 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
    50341 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
    50401 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
    50461 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
    50521 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
    50581 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
    50641 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
    50701 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
    50761 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
    50821 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
    50881 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
    50941 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
    51001 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
    51061 tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt
    51121 ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac gaagtttact
    51181 ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt tgataactat
    51241 cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat atggtaaaca
    51301 gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt cttttaaatt
    51361 gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat agttgacgaa
    51421 acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct atagaaaagg
    51481 agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag aaaaaaccgc
    51541 tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg ctgaagtgtt
    51601 agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg aaggaaaact
    51661 gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta cgcacgtaaa
    51721 gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag aaaaaaatac
    51781 actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg gcagcatgaa
    51841 aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat taaattgttt
    51901 gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt atcattttgc
    51961 aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt gtgctgatta
    52021 tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag gtgcttttac
    52081 aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta tgctgttttt
    52141 ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact ttatggatca
    52201 tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg tacggatcgt
    52261 aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc ggcgtatccc
    52321 aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag tcgatttagt
    52381 aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt tgacagaaga
    52441 tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat tgaagtcaaa
    52501 tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg aaatctcgat
    52561 cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag caagcaaccg
    52621 cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag caccaaatga
    52681 tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat atgatatcat
    52741 cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa tcaatcattt
    52801 catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg gtttttcttt
    52861 taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag tgaccaatca
    52921 gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact ttatttatgc
    52981 tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag aacggcatac
    53041 gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg ttgcgaaaga
    53101 agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg aagactatta
    53161 cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa tcggtgtagt
    53221 cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc aacagttgtt
    53281 agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga aaacagcatt
    53341 ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca tactcttagt
    53401 agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg gaattccagt
    53461 acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa aggtctcagt
    53521 attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag gttacgcaga
    53581 aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa aagctgtttt
    53641 ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga tcgaacgagc
    53701 ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg tggaagtgaa
    53761 agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg ggattactga
    53821 tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa atatcgaagt
    53881 cattttggat cagttgctga tggaatcaga attggaggaa actgaattca tctccctaga
    53941 tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca tctttattaa
    54001 tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc agacgacggg
    54061 aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag caggaatgat
    54121 cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga caaatacaac
    54181 gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag atcagataaa
    54241 agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata atcagttaga
    54301 taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat agtgtatcgt
    54361 ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa aagttggcac
    54421 gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa tgataggggt
    54481 ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg gcacgtatag
    54541 atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac
    54601 gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt ctactaaaag
    54661 aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg attcaaatct
    54721 atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat ccccaagatg
    54781 ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt
    54841 ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa gatattgatt
    54901 cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta cctgctgaca
    54961 ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt
    55021 tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt ttgaatttgt
    55081 tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca agtcatggac
    55141 aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa gaaaacgtca
    55201 aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa ttggaagaag
    55261 cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta tggggcggaa
    55321 caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg gcaatcgtca
    55381 gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca atggaatctg
    55441 ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg
    55501 aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa cctaaaggag
    55561 cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg
    55621 actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg caaccaaacc
    55681 gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac
    55741 aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg atcgaagtag
    55801 caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat cctgaagacg
    55861 tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc
    55921 attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa gacgttgaag
    55981 cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta cctaatgatt
    56041 cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca aaagcgtaat
    56101 aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa ttttagtcgt
    56161 attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat ttcatcaacc
    56221 actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg gtatcgtact
    56281 tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc
    56341 agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg gacaaggtgt
    56401 taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag ggttgttctt
    56461 aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg cggctgctga
    56521 agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt
    56581 tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg tgcaatcatt
    56641 ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg gaatggtcgt
    56701 agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat ggccattctt
    56761 cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc taggtgcttt
    56821 aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt catcaaataa
    56881 tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact attaatcttg
    56941 aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga tcgtttagct
    57001 gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat gcaaaacggt
    57061 ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa agaagaacgt
    57121 tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat tgcttctcca
    57181 attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat
    57241 gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt tggtgaccca
    57301 gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt agctatggga
    57361 ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg ttgggcattc
    57421 atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga tgacctttct
    57481 ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt cgtattagct
    57541 gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta
    57601 gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg aatcaagagt
    57661 gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac aacattacaa
    57721 gacaacttag accaattgat tcctggttta gctgcattag gattaacatt gttatgtatg
    57781 tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt cgtagtcggt
    57841 gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg gcgttgtccc
    57901 gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga aaatggttca
    57961 atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg gtctgacaga
    58021 ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc gagatagacg
    58081 caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag tcatgctaaa
    58141 gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat acacattttc
    58201 ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg aagatagaat
    58261 ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg
    58321 caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt actttgcaga
    58381 agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata tgctaaaatt
    58441 gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg ttggagccgt
    58501 gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa
    58561 tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg aatggggacc
    58621 aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt tggtagcagt
    58681 tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa ctaaaaggag
    58741 ttgcagcgag actgtgactc ctttttggct tt
//
LOCUS       DAPBBG010000013        56902 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPBBG010000013 DAPBBG010000000
VERSION     DAPBBG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 56902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.013"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(543..2048)
                     /locus_tag="RD477_000905"
     CDS             complement(543..2048)
                     /locus_tag="RD477_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7633284.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="RD477_000906"
     CDS             complement(2045..2731)
                     /locus_tag="RD477_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7633285.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="RD477_000907"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="RD477_000907"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDT7633286.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="RD477_000908"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="RD477_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDT7633287.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="RD477_000909"
     CDS             complement(4752..5321)
                     /locus_tag="RD477_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDT7633288.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="RD477_000910"
     CDS             5507..6409
                     /locus_tag="RD477_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7633289.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="RD477_000911"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="RD477_000911"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDT7633290.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="RD477_000912"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="RD477_000912"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDT7633291.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="RD477_000913"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="RD477_000913"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDT7633292.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="RD477_000914"
     CDS             12977..13162
                     /locus_tag="RD477_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDT7633293.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="RD477_000915"
     CDS             complement(13224..14420)
                     /locus_tag="RD477_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7633294.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="RD477_000916"
     CDS             complement(14590..16026)
                     /locus_tag="RD477_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDT7633295.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="RD477_000917"
     CDS             16319..17128
                     /locus_tag="RD477_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7633296.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(17314..19467)
                     /locus_tag="RD477_000918"
     CDS             complement(17314..19467)
                     /locus_tag="RD477_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDT7633297.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(19862..20710)
                     /locus_tag="RD477_000919"
     CDS             complement(19862..20710)
                     /locus_tag="RD477_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7633298.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(20934..22874)
                     /gene="thrS"
                     /locus_tag="RD477_000920"
     CDS             complement(20934..22874)
                     /gene="thrS"
                     /locus_tag="RD477_000920"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDT7633299.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(23211..23390)
                     /locus_tag="RD477_000921"
     CDS             complement(23211..23390)
                     /locus_tag="RD477_000921"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDT7633300.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(23437..25260)
                     /gene="cadA"
                     /locus_tag="RD477_000922"
     CDS             complement(23437..25260)
                     /gene="cadA"
                     /locus_tag="RD477_000922"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDT7633301.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            25538..27817
                     /locus_tag="RD477_000923"
     CDS             25538..27817
                     /locus_tag="RD477_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7633302.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(27910..28557)
                     /locus_tag="RD477_000924"
     CDS             complement(27910..28557)
                     /locus_tag="RD477_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633303.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(28736..29455)
                     /locus_tag="RD477_000925"
     CDS             complement(28736..29455)
                     /locus_tag="RD477_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633304.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(29586..30737)
                     /locus_tag="RD477_000926"
     CDS             complement(29586..30737)
                     /locus_tag="RD477_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDT7633305.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(30954..31694)
                     /locus_tag="RD477_000927"
     CDS             complement(30954..31694)
                     /locus_tag="RD477_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7633306.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(31698..32021)
                     /gene="rsfS"
                     /locus_tag="RD477_000928"
     CDS             complement(31698..32021)
                     /gene="rsfS"
                     /locus_tag="RD477_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDT7633307.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(32078..32671)
                     /gene="yqeK"
                     /locus_tag="RD477_000929"
     CDS             complement(32078..32671)
                     /gene="yqeK"
                     /locus_tag="RD477_000929"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDT7633308.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(32655..33305)
                     /locus_tag="RD477_000930"
     CDS             complement(32655..33305)
                     /locus_tag="RD477_000930"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDT7633309.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(33315..33626)
                     /gene="yhbY"
                     /locus_tag="RD477_000931"
     CDS             complement(33315..33626)
                     /gene="yhbY"
                     /locus_tag="RD477_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDT7633310.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(33642..34751)
                     /gene="yqeH"
                     /locus_tag="RD477_000932"
     CDS             complement(33642..34751)
                     /gene="yqeH"
                     /locus_tag="RD477_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDT7633311.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(34748..35275)
                     /locus_tag="RD477_000933"
     CDS             complement(34748..35275)
                     /locus_tag="RD477_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDT7633312.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(35455..36240)
                     /locus_tag="RD477_000934"
     CDS             complement(35455..36240)
                     /locus_tag="RD477_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDT7633313.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(36233..37102)
                     /locus_tag="RD477_000935"
     CDS             complement(36233..37102)
                     /locus_tag="RD477_000935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDT7633314.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(37103..38482)
                     /locus_tag="RD477_000936"
     CDS             complement(37103..38482)
                     /locus_tag="RD477_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDT7633315.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(38490..38909)
                     /gene="fabZ"
                     /locus_tag="RD477_000937"
     CDS             complement(38490..38909)
                     /gene="fabZ"
                     /locus_tag="RD477_000937"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDT7633316.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(38934..39410)
                     /gene="accB"
                     /locus_tag="RD477_000938"
     CDS             complement(38934..39410)
                     /gene="accB"
                     /locus_tag="RD477_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDT7633317.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(39413..40648)
                     /gene="fabF"
                     /locus_tag="RD477_000939"
     CDS             complement(39413..40648)
                     /gene="fabF"
                     /locus_tag="RD477_000939"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDT7633318.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAIS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(40667..41404)
                     /gene="fabG"
                     /locus_tag="RD477_000940"
     CDS             complement(40667..41404)
                     /gene="fabG"
                     /locus_tag="RD477_000940"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDT7633319.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(41416..42333)
                     /gene="fabD"
                     /locus_tag="RD477_000941"
     CDS             complement(41416..42333)
                     /gene="fabD"
                     /locus_tag="RD477_000941"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDT7633320.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(42440..42664)
                     /locus_tag="RD477_000942"
     CDS             complement(42440..42664)
                     /locus_tag="RD477_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDT7633321.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(42717..43679)
                     /locus_tag="RD477_000943"
     CDS             complement(42717..43679)
                     /locus_tag="RD477_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDT7633322.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(43689..44129)
                     /locus_tag="RD477_000944"
     CDS             complement(43689..44129)
                     /locus_tag="RD477_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7633323.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            44435..45400
                     /locus_tag="RD477_000945"
     CDS             44435..45400
                     /locus_tag="RD477_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDT7633324.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(45457..45687)
                     /locus_tag="RD477_000946"
     CDS             complement(45457..45687)
                     /locus_tag="RD477_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDT7633325.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(45773..46660)
                     /locus_tag="RD477_000947"
     CDS             complement(45773..46660)
                     /locus_tag="RD477_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDT7633326.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(46749..47972)
                     /locus_tag="RD477_000948"
     CDS             complement(46749..47972)
                     /locus_tag="RD477_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7633327.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(47989..49035)
                     /locus_tag="RD477_000949"
     CDS             complement(47989..49035)
                     /locus_tag="RD477_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7633328.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(49199..50926)
                     /gene="ptsP"
                     /locus_tag="RD477_000950"
     CDS             complement(49199..50926)
                     /gene="ptsP"
                     /locus_tag="RD477_000950"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDT7633329.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(50926..51192)
                     /locus_tag="RD477_000951"
     CDS             complement(50926..51192)
                     /locus_tag="RD477_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDT7633330.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            51671..53905
                     /locus_tag="RD477_000952"
     CDS             51671..53905
                     /locus_tag="RD477_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7633331.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            54053..54373
                     /locus_tag="RD477_000953"
     CDS             54053..54373
                     /locus_tag="RD477_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDT7633332.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(54578..54802)
                     /locus_tag="RD477_000954"
     CDS             complement(54578..54802)
                     /locus_tag="RD477_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDT7633333.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(55000..56580)
                     /locus_tag="RD477_000955"
     CDS             complement(55000..56580)
                     /locus_tag="RD477_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDT7633334.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT        16199 a        12175 c         9862 g        18666 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg
    17221 tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac
    17281 tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata
    17341 cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt
    17401 cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt
    17461 actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt
    17521 tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc
    17581 atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac
    17641 cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc
    17701 attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt
    17761 tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc
    17821 ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact tcttcatata
    17881 atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc
    17941 aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa
    18001 actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat
    18061 catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct
    18121 tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa
    18181 aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac
    18241 tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt
    18301 ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat
    18361 agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata
    18421 attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa
    18481 attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc
    18541 aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa
    18601 ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc
    18661 atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact
    18721 tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc
    18781 gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt
    18841 gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc
    18901 gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt
    18961 ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc
    19021 agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat
    19081 cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct
    19141 cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta
    19201 gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc
    19261 ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa
    19321 taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa
    19381 cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag aagtatttaa
    19441 tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt
    19501 cattgtaccc aaaaacttct taataaacaa gaagtattta atattttttt cataaaatct
    19561 ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc caaaaacttc
    19621 ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc aagataaaat
    19681 caactttttg tttcacagca atcacttaaa gttacatatc caactatttt tttcattaaa
    19741 aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt gatggatcat
    19801 ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa tagtaaggtt
    19861 tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
    19921 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
    19981 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
    20041 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
    20101 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
    20161 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
    20221 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
    20281 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
    20341 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
    20401 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
    20461 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
    20521 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
    20581 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
    20641 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
    20701 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
    20761 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
    20821 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
    20881 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
    20941 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
    21001 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
    21061 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
    21121 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
    21181 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
    21241 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
    21301 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
    21361 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
    21421 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
    21481 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
    21541 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
    21601 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
    21661 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
    21721 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
    21781 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
    21841 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
    21901 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
    21961 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
    22021 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
    22081 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
    22141 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
    22201 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
    22261 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
    22321 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
    22381 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
    22441 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
    22501 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
    22561 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
    22621 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
    22681 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
    22741 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
    22801 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
    22861 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
    22921 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
    22981 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
    23041 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
    23101 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
    23161 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
    23221 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
    23281 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
    23341 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
    23401 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
    23461 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
    23521 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
    23581 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
    23641 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
    23701 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
    23761 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
    23821 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
    23881 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
    23941 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
    24001 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
    24061 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
    24121 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
    24181 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
    24241 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
    24301 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
    24361 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
    24421 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
    24481 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
    24541 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
    24601 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
    24661 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
    24721 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
    24781 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
    24841 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
    24901 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
    24961 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
    25021 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
    25081 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
    25141 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
    25201 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
    25261 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
    25321 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
    25381 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
    25441 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
    25501 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
    25561 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
    25621 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
    25681 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
    25741 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
    25801 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
    25861 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
    25921 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
    25981 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
    26041 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
    26101 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
    26161 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
    26221 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
    26281 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
    26341 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
    26401 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
    26461 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
    26521 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
    26581 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
    26641 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
    26701 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
    26761 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
    26821 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
    26881 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
    26941 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
    27001 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
    27061 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
    27121 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
    27181 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
    27241 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
    27301 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
    27361 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
    27421 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
    27481 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
    27541 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
    27601 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
    27661 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
    27721 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
    27781 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
    27841 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
    27901 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
    27961 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
    28021 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
    28081 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
    28141 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
    28201 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
    28261 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
    28321 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
    28381 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
    28441 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
    28501 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
    28561 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
    28621 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
    28681 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
    28741 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
    28801 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
    28861 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
    28921 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
    28981 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
    29041 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
    29101 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
    29161 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
    29221 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
    29281 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
    29341 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
    29401 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
    29461 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
    29521 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
    29581 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
    29641 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
    29701 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
    29761 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
    29821 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    29881 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    29941 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    30001 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    30061 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    30121 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    30181 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    30241 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    30301 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    30361 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    30421 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    30481 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    30541 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    30601 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    30661 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    30721 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    30781 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    30841 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    30901 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    30961 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    31021 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    31081 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    31141 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    31201 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    31261 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    31321 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    31381 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    31441 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    31501 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    31561 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    31621 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    31681 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    31741 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    31801 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    31861 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    31921 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    31981 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    32041 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    32101 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    32161 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    32221 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    32281 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    32341 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    32401 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    32461 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    32521 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    32581 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    32641 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    32701 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    32761 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    32821 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    32881 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    32941 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    33001 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    33061 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    33121 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    33181 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    33241 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    33301 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    33361 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    33421 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    33481 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    33541 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    33601 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    33661 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    33721 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    33781 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    33841 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    33901 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    33961 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    34021 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    34081 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    34141 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    34201 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    34261 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    34321 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    34381 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    34441 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    34501 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    34561 aaaaagtcgt catccgtcaa tgagacatct tggatctcat tataatggcg taatctgaaa
    34621 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    34681 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    34741 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    34801 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    34861 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    34921 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    34981 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    35041 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    35101 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    35161 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    35221 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    35281 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    35341 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    35401 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    35461 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    35521 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    35581 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    35641 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    35701 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    35761 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    35821 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    35881 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    35941 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    36001 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    36061 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    36121 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    36181 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    36241 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    36301 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    36361 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    36421 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    36481 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    36541 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    36601 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    36661 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    36721 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    36781 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    36841 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    36901 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    36961 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    37021 caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt
    37081 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    37141 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    37201 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    37261 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    37321 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    37381 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    37441 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    37501 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    37561 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    37621 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    37681 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    37741 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    37801 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    37861 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    37921 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    37981 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    38041 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    38101 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    38161 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    38221 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    38281 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    38341 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    38401 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    38461 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    38521 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    38581 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    38641 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    38701 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    38761 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    38821 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    38881 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    38941 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    39001 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    39061 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    39121 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    39181 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc agatatgaca
    39241 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    39301 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    39361 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    39421 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttacttattg
    39481 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    39541 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    39601 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    39661 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    39721 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    39781 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    39841 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    39901 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    39961 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    40021 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    40081 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    40141 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    40201 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    40261 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    40321 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    40381 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    40441 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    40501 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    40561 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    40621 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    40681 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    40741 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    40801 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    40861 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    40921 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    40981 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    41041 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    41101 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    41161 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    41221 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    41281 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    41341 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    41401 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    41461 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    41521 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    41581 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    41641 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    41701 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    41761 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    41821 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    41881 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    41941 cactgcaacc attttcccgc ttccagctgg ggtagcttct gtcatataag cgcctctttt
    42001 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    42061 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    42121 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    42181 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    42241 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    42301 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    42361 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    42421 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    42481 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    42541 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    42601 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    42661 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    42721 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    42781 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    42841 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    42901 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    42961 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    43021 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    43081 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    43141 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    43201 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    43261 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    43321 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    43381 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    43441 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    43501 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    43561 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    43621 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    43681 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    43741 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    43801 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    43861 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    43921 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    43981 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    44041 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    44101 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    44161 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    44221 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    44281 catttataat ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta
    44341 ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca aaatttgtta
    44401 tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct
    44461 ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca tctatgttgg
    44521 taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc
    44581 aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac
    44641 ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa
    44701 ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata agggatttgt
    44761 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
    44821 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
    44881 gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
    44941 ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag
    45001 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
    45061 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc
    45121 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
    45181 ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta
    45241 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
    45301 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga
    45361 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta
    45421 ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc
    45481 catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa
    45541 ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc
    45601 aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat
    45661 gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt
    45721 gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt
    45781 ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat
    45841 tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca
    45901 atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt
    45961 tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag
    46021 accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt
    46081 ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct
    46141 gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac
    46201 caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga
    46261 tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat
    46321 ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtacttgtag
    46381 tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt
    46441 agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg
    46501 ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg
    46561 cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag
    46621 cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat
    46681 caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg
    46741 tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct
    46801 tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc
    46861 atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga
    46921 tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt
    46981 tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc
    47041 acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa
    47101 gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc
    47161 acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac
    47221 tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg
    47281 acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat cctgcttaaa
    47341 gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc
    47401 tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga
    47461 agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact tggacaaact
    47521 acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc
    47581 acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc
    47641 agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta
    47701 tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct
    47761 ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca
    47821 tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca
    47881 tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga
    47941 aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac
    48001 ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt
    48061 tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg
    48121 tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt
    48181 tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc
    48241 tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc
    48301 ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc
    48361 acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat
    48421 gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat
    48481 cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca
    48541 aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat
    48601 tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg
    48661 gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc
    48721 tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat
    48781 aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc
    48841 tcttctggct ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat
    48901 gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt
    48961 ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata
    49021 taataatacc ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact
    49081 gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt tttcttaaaa
    49141 aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt
    49201 attttacata ttcatgaaca agttcaacga cttcttccat catatcgcat tctttcaatg
    49261 cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt
    49321 taagaataga tgtcgcactc attgagaact catctagacc catacctact agcaatggaa
    49381 cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg
    49441 ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt
    49501 aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac
    49561 ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa
    49621 tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca cttatcaatt
    49681 tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca
    49741 tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg
    49801 tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga
    49861 atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca
    49921 ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat
    49981 attggtcatc ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca
    50041 gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt
    50101 ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt
    50161 cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt
    50221 catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt
    50281 ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct aaagaacgtg
    50341 ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat
    50401 tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt
    50461 cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag
    50521 caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga
    50581 acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt
    50641 ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg
    50701 catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct
    50761 gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat
    50821 cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa
    50881 cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc
    50941 ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc
    51001 agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt
    51061 caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt
    51121 ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc
    51181 tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc
    51241 ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat
    51301 tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc
    51361 cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct
    51421 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
    51481 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
    51541 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
    51601 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
    51661 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
    51721 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
    51781 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
    51841 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
    51901 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
    51961 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
    52021 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
    52081 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
    52141 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
    52201 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
    52261 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
    52321 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
    52381 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
    52441 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
    52501 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
    52561 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
    52621 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
    52681 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
    52741 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
    52801 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
    52861 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
    52921 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
    52981 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
    53041 ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
    53101 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
    53161 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
    53221 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
    53281 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
    53341 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
    53401 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
    53461 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
    53521 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
    53581 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
    53641 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
    53701 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
    53761 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
    53821 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
    53881 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
    53941 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
    54001 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
    54061 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
    54121 ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga
    54181 acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga
    54241 tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa
    54301 agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac
    54361 attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga
    54421 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    54481 aaataagcta aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct
    54541 tggagttaaa cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt
    54601 tcttttgtac ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt
    54661 cctctttatg gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt
    54721 ttacatctaa taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac
    54781 gtatttgcca ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa
    54841 ataattttta tatttttttg attagacatt tctatgattt taatatagta aaaagagccg
    54901 tcacggaaaa tccgtcacgg ctctaaacaa aatatgagag gctgtttttg tttggctatt
    54961 cttattactt gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt
    55021 tacattagga tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa
    55081 tggttggtcg aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa
    55141 atcttctgga tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc
    55201 gttatactca tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc
    55261 aatgatatat tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa
    55321 ttgatagatt cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat
    55381 aaacagctct ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa
    55441 caatgtatcc ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac
    55501 tgctttttcg acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag
    55561 ttttttattt gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat
    55621 tcggacaaaa gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac
    55681 gaaacctgaa aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc
    55741 ataaggagct ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt
    55801 caatgcagaa ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa
    55861 tgcatctcca gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt
    55921 ttcagttaga ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc
    55981 atcctgataa ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc
    56041 ttctagccct ttacccatac cgattggcca attcatcgga taagattcga tatctaatag
    56101 ctcttctaat tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat
    56161 aaaagtaaat atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc
    56221 ttcgatccct tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg
    56281 ataagtatct tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc
    56341 ttgataatca aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc
    56401 catccagtct gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg
    56461 gatagcccca ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg
    56521 ggaaatgatc gcaaaagttc tgcgattgtc cacttgttgc tttaattctg gattattcat
    56581 aacctaactc catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga
    56641 tctcttaaag aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt
    56701 ttccgagtaa acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa
    56761 tacgaaaagc tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca
    56821 acctttgtca actactacaa ctgaatcagt atagcatgga ttcatcgaat gaaaaagcca
    56881 ccaaaccata agagtcaaat at
//
LOCUS       DAPBBG010000014        54570 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPBBG010000014 DAPBBG010000000
VERSION     DAPBBG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 54570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.014"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            319..627
                     /gene="rpsJ"
                     /locus_tag="RD477_000956"
     CDS             319..627
                     /gene="rpsJ"
                     /locus_tag="RD477_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDT7633335.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            658..1287
                     /gene="rplC"
                     /locus_tag="RD477_000957"
     CDS             658..1287
                     /gene="rplC"
                     /locus_tag="RD477_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDT7633336.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1315..1938
                     /gene="rplD"
                     /locus_tag="RD477_000958"
     CDS             1315..1938
                     /gene="rplD"
                     /locus_tag="RD477_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDT7633337.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1938..2228
                     /gene="rplW"
                     /locus_tag="RD477_000959"
     CDS             1938..2228
                     /gene="rplW"
                     /locus_tag="RD477_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDT7633338.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2267..3100
                     /gene="rplB"
                     /locus_tag="RD477_000960"
     CDS             2267..3100
                     /gene="rplB"
                     /locus_tag="RD477_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDT7633339.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3143..3421
                     /gene="rpsS"
                     /locus_tag="RD477_000961"
     CDS             3143..3421
                     /gene="rpsS"
                     /locus_tag="RD477_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDT7633340.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3444..3791
                     /gene="rplV"
                     /locus_tag="RD477_000962"
     CDS             3444..3791
                     /gene="rplV"
                     /locus_tag="RD477_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDT7633341.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3805..4461
                     /gene="rpsC"
                     /locus_tag="RD477_000963"
     CDS             3805..4461
                     /gene="rpsC"
                     /locus_tag="RD477_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDT7633342.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4464..4898
                     /gene="rplP"
                     /locus_tag="RD477_000964"
     CDS             4464..4898
                     /gene="rplP"
                     /locus_tag="RD477_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDT7633343.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4888..5076
                     /gene="rpmC"
                     /locus_tag="RD477_000965"
     CDS             4888..5076
                     /gene="rpmC"
                     /locus_tag="RD477_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDT7633344.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5101..5367
                     /gene="rpsQ"
                     /locus_tag="RD477_000966"
     CDS             5101..5367
                     /gene="rpsQ"
                     /locus_tag="RD477_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDT7633345.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5425..5793
                     /gene="rplN"
                     /locus_tag="RD477_000967"
     CDS             5425..5793
                     /gene="rplN"
                     /locus_tag="RD477_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDT7633346.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5830..6138
                     /gene="rplX"
                     /locus_tag="RD477_000968"
     CDS             5830..6138
                     /gene="rplX"
                     /locus_tag="RD477_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDT7633347.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6166..6705
                     /gene="rplE"
                     /locus_tag="RD477_000969"
     CDS             6166..6705
                     /gene="rplE"
                     /locus_tag="RD477_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDT7633348.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6724..6909
                     /locus_tag="RD477_000970"
     CDS             6724..6909
                     /locus_tag="RD477_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDT7633349.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6945..7343
                     /gene="rpsH"
                     /locus_tag="RD477_000971"
     CDS             6945..7343
                     /gene="rpsH"
                     /locus_tag="RD477_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDT7633350.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7375..7911
                     /gene="rplF"
                     /locus_tag="RD477_000972"
     CDS             7375..7911
                     /gene="rplF"
                     /locus_tag="RD477_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDT7633351.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8072..8428
                     /gene="rplR"
                     /locus_tag="RD477_000973"
     CDS             8072..8428
                     /gene="rplR"
                     /locus_tag="RD477_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDT7633352.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8449..8949
                     /gene="rpsE"
                     /locus_tag="RD477_000974"
     CDS             8449..8949
                     /gene="rpsE"
                     /locus_tag="RD477_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDT7633353.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8964..9143
                     /gene="rpmD"
                     /locus_tag="RD477_000975"
     CDS             8964..9143
                     /gene="rpmD"
                     /locus_tag="RD477_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDT7633354.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9187..9627
                     /gene="rplO"
                     /locus_tag="RD477_000976"
     CDS             9187..9627
                     /gene="rplO"
                     /locus_tag="RD477_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDT7633355.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9627..10922
                     /gene="secY"
                     /locus_tag="RD477_000977"
     CDS             9627..10922
                     /gene="secY"
                     /locus_tag="RD477_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDT7633356.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10984..11631
                     /locus_tag="RD477_000978"
     CDS             10984..11631
                     /locus_tag="RD477_000978"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDT7633357.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11822..12040
                     /gene="infA"
                     /locus_tag="RD477_000979"
     CDS             11822..12040
                     /gene="infA"
                     /locus_tag="RD477_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDT7633358.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12073..12189
                     /gene="rpmJ"
                     /locus_tag="RD477_000980"
     CDS             12073..12189
                     /gene="rpmJ"
                     /locus_tag="RD477_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDT7633359.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12207..12572
                     /gene="rpsM"
                     /locus_tag="RD477_000981"
     CDS             12207..12572
                     /gene="rpsM"
                     /locus_tag="RD477_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDT7633360.1"
                     /translation="MARIAGVDIPRDKHVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12600..12989
                     /gene="rpsK"
                     /locus_tag="RD477_000982"
     CDS             12600..12989
                     /gene="rpsK"
                     /locus_tag="RD477_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDT7633361.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13069..14007
                     /locus_tag="RD477_000983"
     CDS             13069..14007
                     /locus_tag="RD477_000983"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDT7633362.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14035..14415
                     /gene="rplQ"
                     /locus_tag="RD477_000984"
     CDS             14035..14415
                     /gene="rplQ"
                     /locus_tag="RD477_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDT7633363.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14687..15526
                     /locus_tag="RD477_000985"
     CDS             14687..15526
                     /locus_tag="RD477_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7633364.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15502..16371
                     /locus_tag="RD477_000986"
     CDS             15502..16371
                     /locus_tag="RD477_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7633365.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16361..17158
                     /locus_tag="RD477_000987"
     CDS             16361..17158
                     /locus_tag="RD477_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDT7633366.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17361..18107
                     /gene="truA"
                     /locus_tag="RD477_000988"
     CDS             17361..18107
                     /gene="truA"
                     /locus_tag="RD477_000988"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDT7633367.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18440..21109)
                     /locus_tag="RD477_000989"
     CDS             complement(18440..21109)
                     /locus_tag="RD477_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDT7633368.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21347..21535)
                     /locus_tag="RD477_000990"
     CDS             complement(21347..21535)
                     /locus_tag="RD477_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDT7633369.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21805..22167
                     /locus_tag="RD477_000991"
     CDS             21805..22167
                     /locus_tag="RD477_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7633370.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22219..23901
                     /locus_tag="RD477_000992"
     CDS             22219..23901
                     /locus_tag="RD477_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDT7633371.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            24018..26054
                     /gene="recG"
                     /locus_tag="RD477_000993"
     CDS             24018..26054
                     /gene="recG"
                     /locus_tag="RD477_000993"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDT7633372.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            26103..27104
                     /gene="plsX"
                     /locus_tag="RD477_000994"
     CDS             26103..27104
                     /gene="plsX"
                     /locus_tag="RD477_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDT7633373.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27226..27468
                     /gene="acpP"
                     /locus_tag="RD477_000995"
     CDS             27226..27468
                     /gene="acpP"
                     /locus_tag="RD477_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDT7633374.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            27736..28740
                     /locus_tag="RD477_000996"
     CDS             27736..28740
                     /locus_tag="RD477_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633375.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            28741..29685
                     /locus_tag="RD477_000997"
     CDS             28741..29685
                     /locus_tag="RD477_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633376.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            29685..30647
                     /locus_tag="RD477_000998"
     CDS             29685..30647
                     /locus_tag="RD477_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633377.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            30674..31588
                     /locus_tag="RD477_000999"
     CDS             30674..31588
                     /locus_tag="RD477_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633378.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            31614..33395
                     /locus_tag="RD477_001000"
     CDS             31614..33395
                     /locus_tag="RD477_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7633379.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            33743..34429
                     /locus_tag="RD477_001001"
     CDS             33743..34429
                     /locus_tag="RD477_001001"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDT7633380.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            34449..38030
                     /gene="smc"
                     /locus_tag="RD477_001002"
     CDS             34449..38030
                     /gene="smc"
                     /locus_tag="RD477_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDT7633381.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            38039..38848
                     /locus_tag="RD477_001003"
     CDS             38039..38848
                     /locus_tag="RD477_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7633382.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            38859..39857
                     /gene="ftsY"
                     /locus_tag="RD477_001004"
     CDS             38859..39857
                     /gene="ftsY"
                     /locus_tag="RD477_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDT7633383.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            40044..42311
                     /gene="gshAB"
                     /locus_tag="RD477_001005"
     CDS             40044..42311
                     /gene="gshAB"
                     /locus_tag="RD477_001005"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDT7633384.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(42400..42852)
                     /locus_tag="RD477_001006"
     CDS             complement(42400..42852)
                     /locus_tag="RD477_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDT7633385.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            43002..43952
                     /locus_tag="RD477_001007"
     CDS             43002..43952
                     /locus_tag="RD477_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDT7633386.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            43945..44904
                     /locus_tag="RD477_001008"
     CDS             43945..44904
                     /locus_tag="RD477_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDT7633387.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            44901..45656
                     /locus_tag="RD477_001009"
     CDS             44901..45656
                     /locus_tag="RD477_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7633388.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            45679..46632
                     /locus_tag="RD477_001010"
     CDS             45679..46632
                     /locus_tag="RD477_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7633389.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(47276..47572)
                     /locus_tag="RD477_001011"
     CDS             complement(47276..47572)
                     /locus_tag="RD477_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDT7633390.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            47927..48283
                     /locus_tag="RD477_001012"
     CDS             47927..48283
                     /locus_tag="RD477_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7633391.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            48293..49192
                     /locus_tag="RD477_001013"
     CDS             48293..49192
                     /locus_tag="RD477_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDT7633392.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            49709..50440
                     /locus_tag="RD477_001014"
     CDS             49709..50440
                     /locus_tag="RD477_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7633393.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            50585..52612
                     /locus_tag="RD477_001015"
     CDS             50585..52612
                     /locus_tag="RD477_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDT7633394.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            52720..53817
                     /locus_tag="RD477_001016"
     CDS             52720..53817
                     /locus_tag="RD477_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDT7633395.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            54124..>54570
                     /locus_tag="RD477_001017"
     CDS             54124..>54570
                     /locus_tag="RD477_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633396.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        18242 a         9642 c        11702 g        14984 t
ORIGIN      
        1 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
       61 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
      121 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
      181 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
      241 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
      301 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
      361 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
      421 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
      481 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
      541 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
      601 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
      661 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
      721 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
      781 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
      841 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
      901 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
      961 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
     1021 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
     1081 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
     1141 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
     1201 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
     1261 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
     1321 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
     1381 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
     1441 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
     1501 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
     1561 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
     1621 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
     1681 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
     1741 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
     1801 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
     1861 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
     1921 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
     1981 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
     2041 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
     2101 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
     2161 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
     2221 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
     2281 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
     2341 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa
     2401 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
     2461 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
     2521 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
     2581 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
     2641 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
     2701 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
     2761 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
     2821 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
     2881 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
     2941 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
     3001 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
     3061 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
     3121 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
     3181 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
     3241 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
     3301 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
     3361 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
     3421 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
     3481 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
     3541 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
     3601 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
     3661 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
     3721 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
     3781 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
     3841 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
     3901 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
     3961 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
     4021 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
     4081 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
     4141 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
     4201 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
     4261 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
     4321 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
     4381 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
     4441 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
     4501 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
     4561 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
     4621 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
     4681 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
     4741 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
     4801 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
     4861 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
     4921 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
     4981 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
     5041 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
     5101 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
     5161 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
     5221 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
     5281 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
     5341 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
     5401 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
     5461 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
     5521 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
     5581 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
     5641 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
     5701 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
     5761 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
     5821 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
     5881 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
     5941 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
     6001 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
     6061 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
     6121 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
     6181 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
     6241 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
     6301 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
     6361 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
     6421 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
     6481 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
     6541 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
     6601 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
     6661 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
     6721 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
     6781 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
     6841 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
     6901 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
     6961 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
     7021 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
     7081 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
     7141 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
     7201 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
     7261 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
     7321 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
     7381 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
     7441 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
     7501 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
     7561 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
     7621 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
     7681 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
     7741 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
     7801 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
     7861 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
     7921 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
     7981 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
     8041 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
     8101 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
     8161 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
     8221 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
     8281 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
     8341 agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
     8401 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
     8461 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
     8521 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
     8581 cacgtagggt ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
     8641 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
     8701 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
     8761 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
     8821 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
     8881 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
     8941 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
     9001 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
     9061 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
     9121 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
     9181 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
     9241 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
     9301 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
     9361 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
     9421 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
     9481 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
     9541 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
     9601 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
     9661 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
     9721 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
     9781 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
     9841 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
     9901 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
     9961 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    10021 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    10081 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    10141 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    10201 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    10261 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    10321 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    10381 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    10441 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    10501 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    10561 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    10621 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    10681 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    10741 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    10801 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    10861 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    10921 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    10981 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    11041 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    11101 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    11161 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    11221 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    11281 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    11341 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    11401 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    11461 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    11521 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    11581 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    11641 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    11701 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    11761 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    11821 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    11881 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    11941 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    12001 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    12061 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    12121 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    12181 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    12241 ataaacatgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    12301 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    12361 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    12421 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    12481 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    12541 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    12601 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    12661 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    12721 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    12781 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    12841 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    12901 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    12961 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    13021 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    13081 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    13141 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    13201 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    13261 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    13321 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    13381 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    13441 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    13501 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    13561 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    13621 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    13681 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    13741 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    13801 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    13861 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    13921 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    13981 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    14041 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    14101 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    14161 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    14221 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    14281 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    14341 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    14401 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    14461 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    14521 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    14581 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    14641 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    14701 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    14761 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    14821 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    14881 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    14941 gatttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    15001 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    15061 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    15121 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    15181 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    15241 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    15301 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    15361 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    15421 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    15481 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    15541 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    15601 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    15661 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    15721 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    15781 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    15841 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    15901 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    15961 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    16021 gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag
    16081 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    16141 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    16201 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    16261 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    16321 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    16381 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    16441 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    16501 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    16561 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    16621 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    16681 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    16741 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    16801 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    16861 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    16921 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    16981 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    17041 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    17101 gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac
    17161 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc
    17221 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    17281 atctgaaaag aaagaacgaa aaaattttat agaaaaatca ttattccaga taacctttta
    17341 ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcgtac gacggaacaa
    17401 attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa
    17461 cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg
    17521 ccggagtaca tgcgatcggg caagtaatcc attttgatta tccagaggag cgtccgttag
    17581 aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag
    17641 aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc
    17701 gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt
    17761 acccacttga tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt
    17821 tcacctcttt ttgtgcatca ggaagctcga tagaggataa aatacgtacg atctacgagg
    17881 ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct
    17941 tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat
    18001 tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta
    18061 cagcacaccc tgaaggactt tatttgtatg aagtgaaata tgaataagag gttatgatag
    18121 aagtggtcag cttcaagaaa taagaaggta tttacgaaaa ttacttctca aaattttgca
    18181 aaatttcggc ttattttcga agaagctact tctggaacac cgtttaagaa agaggtcgtg
    18241 agcctgtcat ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg
    18301 atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt
    18361 ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg
    18421 aacaggttcc atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt
    18481 cttctattgc tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat
    18541 aaagaataaa caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag
    18601 gcaagtaact gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt
    18661 ctttgaaatt gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat
    18721 aaggaatagt tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc
    18781 tgccagtggc ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg
    18841 ctacaacatc tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa
    18901 aacttgcagt aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac
    18961 cagaaatgtt gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac
    19021 tttctaattg cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca
    19081 catggacttc ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc
    19141 cagtcgttgt ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg
    19201 taaaatactg ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat
    19261 tatctgtcac tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca
    19321 atgcattgac attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat
    19381 atttactgaa cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca
    19441 tcagcaaagt attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta
    19501 atttatccag gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac
    19561 tgtaaccata gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa
    19621 acgagtcgga atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag
    19681 tatataaact gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg
    19741 tattgaccag tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg
    19801 aaagtttttt atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg
    19861 tcaatacaaa ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg
    19921 ccaccgcaaa gagagcagct acgaccaaaa tgattcctaa caacagccca ccatcgtctt
    19981 gttctatctt ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg
    20041 cataacggaa taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa
    20101 caagataaaa acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca
    20161 acggtacttt ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt
    20221 aaacgaacgg aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga
    20281 ttagatccag ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc
    20341 catttgaccg aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca
    20401 gtaaagaagt gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata
    20461 gacgggcgat aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg
    20521 ataaaaacaa gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac
    20581 gatgaatccc caaaatcact aatggaagat aaacaaatgc atccagccac atgatgatct
    20641 ccgaatgtgc agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc
    20701 agcgaggaat cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct
    20761 taagcaaagt cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca
    20821 gtggcgtgaa gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag
    20881 aagcattcca tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat
    20941 gaaaattaga aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat
    21001 agattccgat acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg
    21061 ctgcgtagcg tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc
    21121 accaaatttc tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa
    21181 gagtctagtt ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat
    21241 aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat
    21301 taaataaaaa accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa
    21361 cttttcctga tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca
    21421 taacgcgaac tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg
    21481 aacggttgtt tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa
    21541 tttcctcctt tgccttgatt tggagcaata gaataattca gctcatacca gactaacata
    21601 tcatatagaa tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa
    21661 taattgtaaa ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt
    21721 aactgccttt tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt
    21781 tttacccgtt aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt
    21841 gaaattacca atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt
    21901 gttgtaggaa tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa
    21961 gaaaattact caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt
    22021 tatacgatta tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa
    22081 gtgaaataca atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta
    22141 caaggtgttc gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc
    22201 tgaaggagga ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg
    22261 gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta
    22321 ttccctgtgc cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct
    22381 aaggcagtag tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa
    22441 ggattgctga tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga
    22501 ttttctaagc aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc
    22561 actcatggag ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg
    22621 acagtagcta gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc
    22681 gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac
    22741 ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt
    22801 gtttatgagg gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa
    22861 ccttctccag cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt
    22921 caaatggcga cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga
    22981 gaaggaccta cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt
    23041 ataggagatt ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca
    23101 gaaaacccag gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa
    23161 gtcgacaaca tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag
    23221 tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa
    23281 ggcgtccaag agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca
    23341 atgaatccaa gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg
    23401 attgtcttgc caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca
    23461 gatatcccag tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt
    23521 tcattcaatg atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct
    23581 agcgttgtga gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag
    23641 atcaaagaag gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat
    23701 atcacagcaa cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta
    23761 acgatcttgg caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta
    23821 acagaaaaac atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct
    23881 tatctatttt ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc
    23941 ataaaaggcc ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa
    24001 ggagggagga aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg
    24061 gagaaaaaag agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga
    24121 cttattatcc ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc
    24181 aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt
    24241 ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt
    24301 tttttaatca accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg
    24361 gtaaatggga tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa
    24421 tggatgactt ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg
    24481 atctaatcaa aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca
    24541 accttgtgga gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc
    24601 caaaagacca cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt
    24661 tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg
    24721 agatccttta tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga
    24781 caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata
    24841 tgcagcgact tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt
    24901 tatacgctac cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg
    24961 cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat
    25021 tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag
    25081 gggaaattaa cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga
    25141 atttaggtct agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac
    25201 ttcgtgaaaa aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa
    25261 cattagctat tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg
    25321 gacgtatccc gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt
    25381 ggacgaaaaa agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag
    25441 aatcagaagc attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat
    25501 ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag
    25561 taatcatgga agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg
    25621 aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg
    25681 gattggcaca gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact
    25741 gtatcttagt tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag
    25801 agaccaccaa tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag
    25861 tatttggctt ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg
    25921 gaaatgtttt ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc
    25981 agatgcttcc tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc
    26041 gttatttcga ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa
    26101 caatgaaaat tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag
    26161 gcgtggcttt agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag
    26221 cagaaatcaa gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa
    26281 aaatcaatag tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg
    26341 tccttgctgc acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata
    26401 caggcgcgct actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc
    26461 ctggcttgat gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg
    26521 atcttggcgc taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt
    26581 ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg
    26641 gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg
    26701 aaacaagctt gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg
    26761 atgtagtggt cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag
    26821 caatgaatat gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa
    26881 tgggcgctat gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca
    26941 aacatggcgg tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa
    27001 caggccccga tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg
    27061 tagttggaca attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa
    27121 gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca
    27181 tggtgtaggt aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa
    27241 gtatttaaca aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg
    27301 acggatgaat tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc
    27361 gttttagagc ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt
    27421 gaaacagtcg gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa
    27481 gcaaagaagg ataaaaagct gcatgcagct ttttatcctt ctttttttaa tctttttgac
    27541 acgtaaaagt catttttctg ataattctgg cgataaacag aaattatgtt gcaaagtaaa
    27601 gcggttttct atataattat taataattca ttacacaaag ataagaataa cctttgttat
    27661 aaaacgattt attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca
    27721 gagaggggta ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga
    27781 ttccgactca aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa
    27841 aacgagattt tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg
    27901 attatgggat tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat
    27961 ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga
    28021 atgattttcc aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc
    28081 aaagaaggac tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta
    28141 gaattgctag aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat
    28201 gaactatcag gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct
    28261 ccaatcgtca ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt
    28321 cttgatttac tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat
    28381 gacttaggag tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc
    28441 gttgaagtag caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct
    28501 ttactgaact ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt
    28561 gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca
    28621 tggataccag caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac
    28681 ttggtacgtt gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag
    28741 atgacagaat taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc
    28801 ttcaatcgag taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa
    28861 ggaaagacat atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc
    28921 gttgtgggat tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg
    28981 aagcgaagca accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat
    29041 tcaatgtcca gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga
    29101 aattttgaac ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc
    29161 gttgggatgc cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt
    29221 caacgtctag gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa
    29281 cctgtatctg ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc
    29341 cagcaagaat acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac
    29401 atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa
    29461 gacatttata atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa
    29521 atcgatgtag aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat
    29581 atccaaaacc aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc
    29641 acaactacac acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc
    29701 tgcgacgtgt tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat
    29761 tagcccaagc gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg
    29821 cagtgatcga acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact
    29881 tccgttggat cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct
    29941 ctgttgctga cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag
    30001 ttgtccttac ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt
    30061 caatcttgga taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta
    30121 tcttcggctt gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg
    30181 gatcacaaac actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat
    30241 atattctgtt accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc
    30301 tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag
    30361 gggtaccgac gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg
    30421 cttctcaaat ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga
    30481 tttttggtta tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata
    30541 ccgtgatcac atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt
    30601 cagatatcat catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa
    30661 ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat
    30721 ggggatacga atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg
    30781 tttgctagtg gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa
    30841 agtgatgtat gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg
    30901 acaaaagttc attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat
    30961 aggtgctaga aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg
    31021 ggttggatta gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg
    31081 gatcgttgat tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat
    31141 tatttccaga tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc
    31201 taaagcccga ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc
    31261 ctcaaaaaca atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct
    31321 aagttcatta atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac
    31381 aactttgact ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat
    31441 cggttatgca agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc
    31501 aatccttatt ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc
    31561 tgcagatgcg cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga
    31621 aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg
    31681 gaggtacaga tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa
    31741 cttttccaat tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg
    31801 cggtcgcaac ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat
    31861 atgatgcagc ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca
    31921 gaatcacaga cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa
    31981 tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga
    32041 ttttccctta cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt
    32101 tccgtaacat cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa
    32161 tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc
    32221 ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag
    32281 caatcaaaga tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt
    32341 attacatgag taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact
    32401 ggaatggtac gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa
    32461 ctgaagcatt gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg
    32521 atacctataa agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt
    32581 atctaggatt caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag
    32641 attcaaaaat ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg
    32701 cagtaggtga acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag
    32761 tattccagtc ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga
    32821 aaaaactatt agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca
    32881 aagatggaaa taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac
    32941 cattggcaga ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa
    33001 caggtcgtct gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa
    33061 ttgatgttta ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg
    33121 gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca
    33181 acgatatcag ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat
    33241 ggcaacaata tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat
    33301 tgccagtcaa cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg
    33361 caaatgtttc tgttacagca gattcacgtt cataatcact aaaaatgacc gaaaaagggc
    33421 gatgtccttt ttcggttttt tgatattttt ctgtttattt ctctaatatt caggtcaagg
    33481 cgctgatttc atgacttctc caataaacgg tgttcaaagt ccagtttctc ggtattaagt
    33541 caaatttatg caaaaatatg gaaagccatt ttcacaaaat tttttcttca tactcgaaac
    33601 taaacgaatt tttcacaact tctcgtcaat cactaggatt attaaagctt tacttacctt
    33661 ttgaccgaac catttcaaat ttgacaaaaa accagtataa taaggaagta tcgaaaaaat
    33721 atccataaaa ggaatgtgga aaatggacaa ccagctgaca aaagaactaa aagaaaacta
    33781 taacatcgtt tttcacgatc taaatttgct tgaacaagct tttacccatt catcctatgt
    33841 gaatgagcat cgcaatctgc aattatcgga taatgagcga cttgaatttt taggagatgc
    33901 cgttttagaa ctgatggttt ctcagtattt attccgttta tatcctcatt tgccagaagg
    33961 aaaattgaca aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa
    34021 agaatgtcac tttgatcaat atgttttgtt gggcaaggga gaagagaatt caggaggacg
    34081 tacgcgacca gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt tatatcttga
    34141 ccagggattc gacacgaccc atgcattcat cgaaaaagtg atttttccta aagtcaaagc
    34201 aggtgctttt tcacatgaga tggatcataa aacaaaacta caagaagtct tgcaaaaatc
    34261 aggtgatgta tcaatcgaat accgattgat caatgaagaa ggtccagctc atgaacgagt
    34321 attttggatc gaagtttatg tagatgatca attgattggc actggacaag ggaaatcgaa
    34381 aaaattagca gaacaagctg cggcggaaaa tgcattggca gcactgtaaa agtaagtgag
    34441 ggaaagcttt gtatttgaaa cgaattgaaa tagccggttt taaatcattt gcagacaaga
    34501 cagtcattga ttttgaaaat agcgtcacag cagtggttgg accaaatggc agcggaaaaa
    34561 gcaatattac agaggctatc cgctgggtat taggagaaca atctgctaaa agtctgcgag
    34621 gcggaaaaat gccggatatc atctttgccg gttccgatac ccgtaagcag ctgaatattg
    34681 cagaagtcac agtgatttta gacaatagtg atcattatct gccattggag tacaatgaga
    34741 tcagtgtaac ccgccgttat cgacggactg gggaaagcga gtttttcatc aataaacaaa
    34801 gctgcagatt aaaagatatc caagaattgt tcttagattc tggattagga aaagaatcgt
    34861 tctcaatcat ttctcaagga aaagtagaag cgattttcag tagtaaaccg gaagaccgtc
    34921 gtgggatctt tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag
    34981 aacagaagct ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc atccacgaat
    35041 tggaagaaca gttaactcct ttggccgccc agagtgaggc agcaaaggag tttttacggc
    35101 taaaagaaac actgactcaa accgatgtct ctttgatggt tgctgaaatc aagacagcaa
    35161 aaaaagattg ggacaacaaa caagcacagc ttgcgaaatt caatctagaa ttaggcaaac
    35221 tatctgagtc gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc
    35281 aagccgatcg tctgatcgaa aaaaatcagc aagtattatt agatctttcg gaaaaactga
    35341 agcagacaga aggacaaaaa gatgttttgc aggagcggac aaagcataca caaaaaagct
    35401 cgcaagaata tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc
    35461 ttcaagaaag cttgatgaaa gcagcagcag aaaaagaaac agagatccaa aaagctgaag
    35521 caaatctcat aaaaacacag caagaattag aaaaatacca aaaatcaacg aaagaattat
    35581 tggcagaatt aagagatcaa tatgttgatt tgatgcaaga acaagcagct gtaggaaacg
    35641 aactgaagta tctagaacgt caatatatac aagaaacagc aaaaagcaag cagacgttag
    35701 ccaagcaatc ggaagtagaa gcatcagtag accgattgat cttacaaaaa gaagaattga
    35761 cacaaaagca ggcgcagcta aaatcatctt taactgaaac aaaagagaag ttagaaatga
    35821 tccaacaaaa cgggaaaaag ttccaagaag cattagcaaa agaacagcct aaaatgtatc
    35881 aattaatgaa tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag
    35941 aaaactattt tggtttttac caaggggttc gattggtctt gcagcataaa cagcagcttt
    36001 cgggtattgt cggagcagtg gctgaactga tcgatgttcc tgcagatttc acgttagcta
    36061 tcgaaacagc tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc
    36121 ggcaagccat cacgtatttg aaacagcaaa gaggaggaag agcaacattc cttccactga
    36181 cgacaatcaa accaagacaa ttaccggctc atatattgac acaagcagca gctgtagaag
    36241 ggtttatagg gattgccagt gaacaagtca gttatcccga tcaaatccaa actgtggttc
    36301 aaaacctgct aggaacgatc cttttagcca aagacttaac tagtgccaat gcaatcgcgc
    36361 aaacgatccg ctatcaatat cgagtggttt ccttagaagg tgatgtgatg aatgccggag
    36421 gatccatgac aggtggagcg aataaacgag gaaaccaagg cagtttgttt gtacaaaatc
    36481 aagaattgaa acagttgact agtgaatttg aagaagcaga taaacaactc caagcacaag
    36541 aaaaaaaagt tcaggagctg caacaagaaa cagctcgttt agccgaagaa caagaagttt
    36601 tgcgtacacg aggcgaacaa ttacgttttg aagaacaaga agcgacaaat cagctacaaa
    36661 acatcataaa tgaacttgaa cgttttgaaa aagaaaagca aatctcaact tttgagacac
    36721 gtgaactgca gcagtttatc gaagattacg aaaagcaaac gaacgaattg aaagacaaac
    36781 aaacagatct tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag
    36841 aaagcgacca gatggaagcc agaagagcac aagtacaaag tcaaaaagca caagaacaag
    36901 cagatcttgc tgtcttgaaa gagcagttta atcatctgca gatccagctc cgcggtgccc
    36961 gagtccaaaa agcagaggca accgaacgtc aagaagcgat agaaaagcag ttggcgacat
    37021 tgacagcaga cttttctgat catgaagtca cagaagaaag tttggaaaaa caaattaacg
    37081 aattatctgc tcagcgagaa acattaaaag ccgagttggc aaaagccaaa gagcagcgag
    37141 atagaaggca aaaggagatc gataagcttg aagcagtctt ggcagagcgt aatcgtgagc
    37201 aaaaagcacg actgtcggaa caatccaaat tagaagtcca aaaagaccga gctgaaatga
    37261 ttttagataa tcatttgacc tatctgcagt cagaatatca gctgacattt gagaaagctt
    37321 cccaagacta tcaagaaaca accgatatcg aagacagtag aacaaaggtt tcatccttaa
    37381 aagaacagat tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa tacgaacaag
    37441 tcagtgaaag acatacattc ttagcaacac agcgagatga tctgttagca gcgaaaaacc
    37501 aattgtttga aacgatggat gagatggatg atgaagtgcg gacaagattc aaagaagtat
    37561 ttgaagctat ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag
    37621 ctgaattagt actgacggaa ccaactgatt tgctgaaaac aggtattgag attgaagtac
    37681 aacctcctgg caaaaaactg caaagtctaa gtttgctttc tggaggagaa cgagcactta
    37741 cagccatcgc gttgctcttt tccattatcc gtgtctgtcc tgttccgttt tgtatcttag
    37801 atgaggtaga agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg
    37861 atttccaaga tgacacacaa tttatcgtag tcacacaccg caaaggaacg atggtcgcag
    37921 cagatgtact gtacggagtg acgatgcagg aatccggtgt atcaaaaatc gtttctgtac
    37981 ggatggaaga tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat
    38041 gatcaaatta gtagcaatcg atttggacgg tacattatta aatgaacaaa agctgatttc
    38101 agatgagaat aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg
    38161 tacaggacgc cccttagctg cgatggcgca ttatctgcaa gaattaggac tggtagatga
    38221 aggtgactac agcatcactt tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat
    38281 catggaaaag aaagtgatgg aagtatcaga tatccaacgc ttatacaaac tggctcagca
    38341 attggatctg ccactagatg ttttgtctga caatgtggtt ttacagctgc ctagtgcccc
    38401 aaggaagcaa tccttgtaca atgtgttgaa taagctgctg caattccaac cagcggatat
    38461 aagcgatatt acagatgaat ttgtgcttaa taaggcagta atcgcttatg accaagaaga
    38521 attagatcct aaaatcaaag aaataccagc agaattccat gaaatgtatg agatcatcaa
    38581 aacaagaagt atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt atggtatttc
    38641 tttattggca aaagatcttg gtttgaaaca agaagaaatc atggcgatcg gtgatgagga
    38701 aaatgattta ccgatgatcc aatatgctgg actaggcgta gcgatggaga atgctgtgcc
    38761 tttcgtcaaa gaagcagcag atttcgtgac agccagcaat gaagaaaatg gcgtagccaa
    38821 agtgatccaa aaatttgtat tagagtaggg ggagtcaaat gggatttttt gataaaatca
    38881 agcgtgcttt tactggcgaa gagaaaacag aagaagttga agtacaggag aagtacgaca
    38941 agggtttaga aaagacgcgt aaaacatttg gtgaacggat gaatgagttg tttgccaatt
    39001 ttcgaacagt cgatgaagac ttttttgaag aattagaaga aacacttatc ggtgcagatg
    39061 taggttttga aacagcgata cgaattaccg aagcattacg tcaagaagtg aaactcagaa
    39121 atgcgaaaaa atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg gatctctatg
    39181 aagcggaagg aatcaatgaa gtaaatgaac taaatatcca agaagacgga ctaacagtta
    39241 ttttgtttgt cggtgttaat ggtgtaggga aaacaacaag tattggtaaa cttgcccatc
    39301 agttcaaaaa cgaaggtaaa aaagtattga tggcagcagc agataccttc cgcgctggag
    39361 caatcgacca gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg
    39421 caggaggcga tccagcagct gtcgtattcg atgctttgga aagagcaaag acagagcagg
    39481 cagatgtttt actagttgat actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg
    39541 aattagaaaa aataaaacga gtaatccagc gggaattacc agaagcaccc caagaaacac
    39601 ttcttgtatt agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa caattcaaag
    39661 aaacaacaga tgtgaccggt cttgttttga caaaattaga tggtacagca aagggcggta
    39721 tcgtacttgc tatccgtaat gaattgcatt taccagtgaa attggtcggt cttggtgaag
    39781 gaatcgatga cttggagccg tttgatccaa atgattttgt cgttggattg ttcaaaggat
    39841 tactaaaaga agaataaccc atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc
    39901 ttgtttgaat gattgttttt aacatagctg tgtttttgat gccttcattt ttatacttat
    39961 ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga ctttttgatt ataattagat
    40021 tcatatgaat tggaggtcac ttaatgatga attttaagca attattattg catgtcaatg
    40081 cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg ggaaggacag cgggttgatc
    40141 ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt tggcgatcga tcctatcacc
    40201 cctatatcca aacagatttt agcgaaacac aaacagagat gatcacccct gttaccgatt
    40261 ctattcccga attatttcag tatctcgctg ctgtttatga tgtgactgct cgttctatac
    40321 cgaaagaaga gatgatctgg ccattaagta tgccacctgc cttaccggaa aaagacgaag
    40381 agatcattat tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa
    40441 aagaatacgg gaagcgaaaa cagatggtaa gcggtattca tttcaatttt gaatttggcg
    40501 atgagttgct aagaacattg ttcagccatc aggaagaatt tcaagatttt tccgaattca
    40561 aaacagaact ttatttgaaa acagcgagaa actttatgcg ctatcgctgg atgatcactt
    40621 atttattcgg tgcttcacca atgagtgaga agaactactt tttagatgaa tcacatccgc
    40681 aagaacctgt tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga
    40741 aagtttccta tgcttctatg aaacagtatt tagcagatat cgagcgaatg atcgaagaag
    40801 ggaaactttc ggaggagaaa gaattttata caccgcttcg tttccgaggc ggaaagaaag
    40861 tcgcagattt agcaacaaca ggtgttcgtt atattgaatt gcggaatatc gatttgaatc
    40921 cttatgcaag attgggtatc aatccagagc aagttcggtt cttacaatta ttcctgatgt
    40981 acatgttatg gacagaagaa aaagaagact gtgaccaatg ggtagcagaa ggtacgactc
    41041 gaaataacaa agtagcacta gaacaaccat cagatcaaac agaatttcat caagaaggta
    41101 gagaaattct tgaaggaatg aagcaaatgc tggttgaatt ggattggttg gattctcttt
    41161 atttagtaga ggaagcattg actcaaatgg atcatcctga acaaacgtta gcagcgaaac
    41221 tctatcaaga agcacagcta tcgagtcagc aagaagttgc tgtggcattg ggacatcaat
    41281 attataaaga aagccatgaa cgtccctatc agttggccgg ttttcgcgaa atggaacttt
    41341 ctactcagat atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg
    41401 agtcggatca atttttgcgt ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa
    41461 acatgacaag taaggacagc tatatcgtcc cacttatcat ggaaaacaaa acagtaacga
    41521 aaaaagtctt aaaagaagct gggttccggg taccaggagg cgcagaattt tcatccatgg
    41581 aagaagcggt aaaagcttat ccgagatttg ccgagcaggc atttgtgatc aaacctaaat
    41641 caaccaatta cgggttaggt atcacgattt tcaaggaagg agctagtctg gaagattatc
    41701 aagcaggttt agccattgct tttcgtgaag acagttcggt tttagtagaa gaatttatgc
    41761 cgggaacgga atatcgtttc tttgtgatcg acggagaagt ccaagccatc atgttgcgag
    41821 tccctgctaa tgttattggt gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa
    41881 atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt agaattgatc caattaggtg
    41941 aattagaaca attgatgttg aaagaacaag ggttgacgat cgaatcagtt ccccaagcca
    42001 atcaaattgt ttatctaagg gagaactcaa atatcagtac aggtggtgat tcgatcgata
    42061 tgactgatga attttctgaa gcttacaaaa aaatcgccgt ttctgcggta gaagcactag
    42121 gagctaagat tagtggtatc gatttgatta taccagataa agaaatcgat cctacaactg
    42181 ataaaaaagc atatgggatc atcgaagcaa atttcaatcc agccatgcat atgcatgttt
    42241 atccttttgc aggaaaaggc agaagattga cgatgaatgt attgaaattg ttatatcctg
    42301 aagtatttta aaagaaaaag cttttgacag attgtcctat ttgatgtcaa tctgatcaaa
    42361 agcttttttt gcatgtaaaa atttatgtgt tttttcgttt taaaataaac gatcccagaa
    42421 gctttttttc ttttttggtt tttctttttc agctggcgaa gagttttctt ctgggtattt
    42481 tttttcgata tcaatcacag gttcatttac tgcttctttt gctgttaaga gcaatccgaa
    42541 ttcctcagga tttgcagtga cgtaatcgtt gactacagtg aaacggctat tgtttttcgt
    42601 tgctagctgg atatattggt tttggacaga aaggggtaga gccccattca agagtatgga
    42661 agcatctgga tgttttttga gttcttttaa aaagttttct ttgttttttg gttggatcat
    42721 ttcagcaaca gtcatactaa gatagcagcg ttccctgaat gtccctaaat atttatgctg
    42781 ttcttcaggg ttcactaaag gtgtaccata aagtccctta tccaaatggc tttttacatc
    42841 atcttgtgcc ataaaaaata ccctccttct tctctttgtc tttcttacga ttatagcata
    42901 ttcccaataa tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg attcacttaa
    42961 aataagaatg aaaatcattc tcaattagaa aggtggattg gatgcgctac ggacgactag
    43021 tagcattatt agttatttta agtagtattt cattgttcat cggagtatcg aatgtacaat
    43081 tgtcagaact gctccagtta gatgggcaaa gcttacaaat cttgctagtg actcgtttgc
    43141 cacgaacgat ttgtttgatc cttgtgggag cgaccagtag tatttgtggg ttgatcatgc
    43201 aacatttgac ccaaaataaa tttgtttcac caacgactgc gggcacgatg gatagtgcgc
    43261 gtttaggtat tttagtggct atgattttcc tacctggtgc atccttgatt gtccgatcct
    43321 tgacagcatt ttgctttgct tttgcaggaa cattgctttt tttatccctt acgcgattgt
    43381 tcctccagaa aaatcaattg atgcttccgt tgattggagt catgttaggg aatatcattg
    43441 gttcagttgc tacttttttt gcttatcaat tccagttggt acaaaatatg acttcatggc
    43501 ttcaagggaa ttttgcgact gtgatacgag gaaattatga gttattgtat gctgttgttc
    43561 cattgttgct cttggtttgg ctatttgctt accgatttac gatcattggt ctgggcgaat
    43621 cgtttgcagt aaatctggga atagattacc agaagatgca gtttttaggc attggcttag
    43681 tatcattggc tagtgcagtc atgttgatca tggtaggaag cattcctttt atcggagtga
    43741 tcattccgaa tcttgtctct agaatatatg gagatcaagt gcacaaaaca attggcgtaa
    43801 ctgctttagc aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt
    43861 ttccttatga aataccagtc agtgtgatcg ttggaatcat cggtggggct atcttccttt
    43921 atcttctgat aaggggacga cgatatgctt aaaaaaaagc catctatttt tattggtatg
    43981 cttgttcttg ttttatttgt tacaggaatg tacttgacga tccatacata tggaaactgg
    44041 cattttgcat gtgcattgag gagtaagaaa atttttgcat ttatccttgt ctcgctagct
    44101 gtcagcagtg ccacaatcag ctttcagaca attacgcata accagttttt gactccttca
    44161 gtattgggac ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt ctttatcgga
    44221 ggagtgacga tgcttcagca gacaggtaca gtttttttct tattgaacgt tgggctgatg
    44281 gtcgggctga gtctattgtt atttttcttt cttttgaaaa aaatgagagc gaatcttttt
    44341 ttattactga tgtcagggat gattttaggt acgctttttg gtagtgtcag taccttttta
    44401 caagtcatca tggatccgaa tgaatatgat ttgcttcaag gcagattatt tgcaagcttt
    44461 ggcaatatcc agagtcagta tcttttgcct gcttctttga tcattatcgg ctgctggtac
    44521 ctgttatttc gatatcgaca tgtgctagac gttttccatc tcggaaatga acaagcagta
    44581 aacctcggga tagacacaga tcgttttgaa tttttgattt tatgttgtgt cagtcttttg
    44641 acagggacat caacggctct tgtgggtcca gttacttttt tagggtttat tgtcgcaaat
    44701 atcagttatc gtgtcttttc tacttatcgt catggtcagc tatttatcgg aagctttctg
    44761 gtagctatgc tgtttctgac aggaggacag ctcgcagtag agcagatttt cggctggaat
    44821 acaacagtga gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac aaaattgatt
    44881 cgcgaaagga agagtatcct atgattgatt tacaaaaggt caataaactg taccatggaa
    44941 aatcagtggt taaagatgta cagttaacga ttcctgaaaa gaaattgact gcttttatcg
    45001 gtccaaatgg tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag
    45061 atacaggtga aatctattta gaccataacg aagtaaaggc ttggaaatcc aatgaattag
    45121 ccaaaaaatt atcgatccta cgacaatcga atgaaataaa tgtccaaatc accgtgaaag
    45181 aattagtaga atttggacga tttccttata cgaaaggaaa attgacgaca aaagacgagg
    45241 aaatcgttca ggaatcactg gaacaccttg ggttagaaga aatagcggat caatcgattg
    45301 acactctttc aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca
    45361 cggattatat tttactggat gaacctttga acaatctaga tatgaatttt gcggttcaaa
    45421 tcatgcagat tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt attgttcttc
    45481 atgatatcaa ctttgctgca agttatgcag acgaaattgt tgcaatgaaa gatggacagt
    45541 tatttacgca aggaacgacc caagaaatga taaaaaaaga tgtattggat gaattatacg
    45601 aaatggacat acggatttgt gagattgaag gccgcagatt ctgtctttat ttctaaaact
    45661 aacattagga gagaagagat gaaaaaatta gtagtaggta tcttgattct agcatcattt
    45721 ggtttagcag catgtggaaa tagcaacaac accagtcagg cagacacaaa aaaaagcagc
    45781 acacaaactg aaactacatt gacgataaca gatagtaatg gagaccaaat cgaggtgcca
    45841 aataatccag aaaaagtcgt cgtattcgac aatggttctt tagatacgat ggatgctctt
    45901 ggtgttggag ataaggtagt tggagcagct acaagcagcc ttcctgagta tctttcatcc
    45961 tataaaaaag tagaatcagc aggtggaata aaagaaccag acttagaaaa aattaatcag
    46021 cttcagccag atctaatcat catttctggc cgacaaagag attttcaaaa agatctaagt
    46081 caaattgctc cgaccatttt cctttcttta gatgcgaaaa atccatggga atctttccag
    46141 caaaatgtca ctgctttagg ggagatcttt ggtaagcaag aagaagctaa aactcaatta
    46201 gaggaattat ctagtgcaat cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa
    46261 gcactagtca cacttgtgaa tgaagggcaa ttatcagcct acggatctgg ttctagattc
    46321 ggtttcatcc atgatttatt tggctttgaa caagcagatg atcaaatcga agcatccact
    46381 cacggtcaaa gtgtttctta tgaatatgtg ctggaaaaaa atccggatat cttatttgta
    46441 gtagacagaa caaaagctat tggtggagac gacagcaaag atgatatttc tgccaatgaa
    46501 ctggtagcac aaacgaatgc tggtaaaaat cagcagatca tctcccttga accagatgtc
    46561 tggtatttaa gcgggggcgg tttagaatca atgaaactga tgatcgaaga tgtgaatcaa
    46621 gcatttaaat agcataaata acagaggatt aaactctcat actatgtaac aataatgttt
    46681 cttacattct taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa
    46741 atcgtattga tgattttacg gatggttttt atttatttga gaaatctttt ttcaacgtga
    46801 attcattatc tacatcatac gggttgattc tagaatgggg agtggcaaaa aatagtgtga
    46861 tccatttttt cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac
    46921 ttagataaaa cttaaattta gatattttta ttttgtaccg atctcttctt taaacgatgt
    46981 tccatctgct gcttctcggc aataagtcca ctagactaga aaatatgaga agctattttc
    47041 tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt ctaaacgatg
    47101 ttccatctgc tgcttctcgg caataagccc actagactag aaaatatgag aagctatttt
    47161 ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt cacaatctct tctaaacaat
    47221 gttcaatcag ctgaccttcg gtattagctc aaagcactga tttcacaacc tctatttagg
    47281 catgtgtctc agcaagcggt tgatagttga tgatctgcat ttttcgataa aaatcggcgg
    47341 gaagcttact ccaccgtttt aataattgat agaaataact ctcttctgct ttgattttat
    47401 actcgcttaa tatcaatcca aaactattcg ttttttttga agctaatttt ttataaatat
    47461 ccaatgcttt ggttttatct tcattgacaa agagtgtttt tacttcaata taagaaccct
    47521 cgatattttg tgtttcttcg ataaaataga tgttttttat atattccttc atagtgattc
    47581 ctccaaagaa gtcttttatt tgataagttt actctaccga agagttgtta ataatccgtg
    47641 aacaaaaagt aattcttttt ccaatagttg ttaaaggttt tttatttttt atagtatttt
    47701 ttgttattta agaaaggaat aaggttaaac aaataacagg ttgctttttg ggtctttttc
    47761 cgttcaagaa actgaacgaa gaaaagggaa agttgtctct tttgtgataa gagaaatcat
    47821 aataaacgaa tgacttactt tccaataaat tgagaacagt cagaaccaga caaattagtt
    47881 gcatctgatg aataaagtga ttattctatg acatgaggtg atacagatga aattagaagt
    47941 gacaaaagaa gcacaagaag tattgaagaa taaattagaa gcagacgatc aacttttatt
    48001 agatatcgaa aacggtgacg gtccatttgc agaaagccaa gtcagttgcc aattggatac
    48061 ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc tttattctgt
    48121 agtggctgat acaccagttg gaccaatcaa aatcaaagac agtgcaatac tttacatgga
    48181 tgatccatcc acacttgctt tagaaccaac ttataatagt ttacaattaa aaggaccaag
    48241 cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac tagagggata aaatgggaaa
    48301 attggcgatc atcagcgacc tacatgtgga catcaataaa ttcggcgaat cagagctgat
    48361 gcagctttgg caagttttgc aaaagcacaa gatcactcgg cttcatctag ccggagatac
    48421 ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata aaggattatc
    48481 tacgacattt catttcggca atcacgagtt ggcagatgtg cccaatgaac agatgatgga
    48541 ccattttcca gacccacatt ttttaaatga acggtatatt gctttaaatg agcagaccgt
    48601 tcttttaggg atgaatggct ggtacgacta tcagttttcc gaattgaaag attacgaggc
    48661 aatcgtacga atgaagcgtt tgtattggta tgatcggttc atccagcgtg ggaaaacaga
    48721 tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc agctggaagc
    48781 aaaaaaactt gaggtgattt tatccactca tttcgttcct aaacgggcat ttatcatgta
    48841 tcaatctgct ccttatgagc gttggaacaa attaaatgca tttttaggat cagaatcatt
    48901 cggagaatta cttgaccgtt attcaaatat caaacaagta gtttttggcc atacgcatcg
    48961 tcgctttgaa gataaatcca tcggtcacac gatttacagc tgcagacctt ttggttatta
    49021 ttatgaatgg cagctgaccc gagattttgt cttgaaagaa cagttgctag acagctatga
    49081 tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg cctatcaaac
    49141 acaacatttg caagaggaat ttgaacaagc gatgacaatc gttccgtatt gagacagcat
    49201 tagtaatgtg taaaagaaaa taccttgtaa aacataagga taagtaagag cagtagatcc
    49261 atttatcgtg gatatgctgc tttttttgcg gaaaataatc agcgaatgtt tatttgatcg
    49321 gatgatttgt aactcgcaga gtactagatg caagcgtgta ttcttttttt gaacgataat
    49381 cacaaaaaaa aatataatat acatttttta tttaattata attaatattg attgaagtct
    49441 aggcagtcaa gtcacatttt gatagatagg agggggactt acgaattatt ttaaaggcta
    49501 tgacagctgt atttctagat tgtgtttgca tcaagttgca ataaaaacta gaaatcaaat
    49561 cgggctgata atcatttata actaatcgat acataggacc aaatataggt ctatgttctt
    49621 ttatttcata agtatcgtaa aacagaattt tgctatggat taatccaatc gactgatatg
    49681 tttagtaaat aaagaaagag gaatgtcaat gatgaaaaaa gaaatcgtat taacagcagc
    49741 aatgatgtta ttcagcatgg tagcaagtac gacttttgtt tctgcaactg aagtataccc
    49801 aaaggaatat aacacagaag gaacgattac atttgaagca ggagatgagg gtgtaactcc
    49861 tcctgttgat ccagagaatc ctgatcccaa taaacctgtc gatccatcag atcctccaag
    49921 ccctggaaca ggaggggcat tatcgattga ttatggatca aaattcaaat ttgggactca
    49981 aaaaatttct actgcggata aaacgtatta tgcagctgct gatgtgatga acgatgggtc
    50041 tagaaaaccg acatatgtcc aagtgacaga taggagaagt accttatctg ggtggaaatt
    50101 aagtgtctct caaccagaac agtttaaaac tgccagtggg gatgaactag ttggagcaca
    50161 gctgaaattt acgaaagggc aggctgtttc tttggttgat ccaacatata cgccgcaaac
    50221 ggtgaattct gaattaacgt tgacaccggg aggaaacaat acgttagcaa ttaatgctaa
    50281 atcaggaaca ggtgtgggga cttgggtcta tcgatttggt gcaaatgaaa atgaaaatca
    50341 agatgcggta cagctttcgg tacccggtaa atctgtcaag ttagcgcaac aatattccac
    50401 taaattagtt tggacattag aagatacacc taacaactag tttactgaaa agaactgtta
    50461 aaaataaata cgtagtcata ttgtcttgcg aggtaatcga gcaaaaattg tcaaaggcta
    50521 ggctaaattt gtttggatac gaatagttca tctaggaatt taacgatcag gaggaaaaga
    50581 tatattgaaa aagaggagga tcgtgctcat tttaagcagt ctcttagtga ctaatatctt
    50641 tccgatcata gggtactctt tggaattgaa tcagttggag catggaaata gaagagacga
    50701 agaaagcaaa ctcacttcga cattcgacag tttaatagaa tccaaccaga cacaagaaag
    50761 cagtgaggat ttaagtattt cgagtagtgg agagaccact gacaacgtag aagctgaggg
    50821 ttcggacaaa caaggactag aagatgcacc aatttctgcg gttgagctgc ctgacgatcc
    50881 caatattatt tctatcgata aaatttttca aacacctatc ggtactagca cgtccatatt
    50941 ggaaaatggg aagttactgc agttgaaccc agcagaaagg tctcaaagag gagctatttg
    51001 gtcaaataag aagattagtt tactctcgga ttttacattt aaatcttatt tatacttagg
    51061 aaatcaacga gatagagcgg gagatggcat gacctttaca ttgactggcg atcctagaat
    51121 ggctagtaat cccagtcaag ttattggatc gccaggtatg ggaattggct catattctac
    51181 tcgttctggc caaccttaca ttgcgaatgc gctctcgatt gagtttgata cgtataaaaa
    51241 tagtggttct tctgatcgaa tggatcgaga gataactgct gataatagag ggcatggaca
    51301 tgtcgctttt gtcacgccta aaccaaataa taataattat tctggagagc atactggcgt
    51361 gacggtagct cctacatttc tttctaatgg cacatggcgc ttgttaataa ttaaatggga
    51421 cgcggagcaa agaagtctta cttatgattt ggaaggtgtc ggtcactctt ctcggattat
    51481 acctgatctc attaggcagt ttggttctac agaagtaaat tgggggttta cttcttcaac
    51541 aggtactttt tttcaagaaa atgctgtcgc tgtcacgcga cttccatcga atgtcacatc
    51601 aacagctgat gtaaaagtca acgatagtga ttatggggcc atcgctgaag ctggaggaaa
    51661 cgataagata actatcagaa atacattgaa ggtcgagggg gattttcttg aatataagaa
    51721 aatgcagact agtatcgacc tcccacaaga attaactatt cccgagcata tcttgatcaa
    51781 tggaaatcaa attgatgcca aagatatctt gataaccgga aatcgagtca cgctaaatct
    51841 gacagattat tttattgctg gagatacgtt aataatcgaa ttggagacaa ctttgcagga
    51901 agtaattcct gaaaaagtac tcactatgca ttttgaatac cttgaaaacg ggaatttgat
    51961 tgagcaatcg aatgaaataa acctaaagat cgcaaagctg aaggagatag atatccatgt
    52021 ttattataaa gataaagaca gtcagattga tattgctgaa atgaaaacaa tcactgggaa
    52081 aataggagag aagtataaag aaactcctat tgatattgaa gggtatgttt ttgactcgga
    52141 ttcagcgaat acagaaggca tcttctccga aaatacagag gatatctatt tttattacag
    52201 aaaaggcaaa ctttatctct caactgctcc tcaatatgtc aattttggaa aacataaaat
    52261 atccagtaga ccattaagtg aattcggaca gttttcaggt gggttgaaaa ttatagatga
    52321 acgggctttt ggaagttgga aattacaatt gaaacaagag aaagttctta caaatggaga
    52381 ttttgagctt ccagaagctc tttcctttgt tacctctgaa aatacaacgg ttatagggac
    52441 atccgctgtt acgattttcg aaagtgatca aaagggtgaa agcgatttgt cctctctttt
    52501 agattctaca gctcatcgtg gaatcagaat agatattcct gtagaaaacc aaagaatcgg
    52561 aacctttgag gggaaactat cttggatact tgcagatgtc ccaggaaatt aacaggaaga
    52621 gaagaatata acaaaattat tgagtttcaa gaagggcaca ggtaccatag gctcttgtgt
    52681 cccacagaga caagattttg ttatagtctt ctcttacata tgattaaagg agcgattttc
    52741 ttgagaccaa aacaacaatt atttataaca ctcctagtta ttttaggggc actttttaac
    52801 aatatatcag tgtcagctag tgcatttaat ttttcagtga cgccgataac ttcagagaat
    52861 caaatcgaca agagaaaaac ttacttcgat cttcaacttg atccggatca ggaagtagaa
    52921 gtaaaagcgg aattgagaaa tgatacagaa aaagaagtga aaatcgatat ttctgtgaat
    52981 agcgcaacca ccaattcaaa tgtgatggtt gaatacggaa aaaacgagat tgaaaaagac
    53041 atgagcctga tctttgatct ggtagattat gtgagctatc cacagatagt gacgctaaaa
    53101 cctaaaagtg tccaaaccgt tgcttttcat gtcaggatgc cgaatgagcg atttgatggt
    53161 gtattggctg gtgggatcac attccaagaa atccagacgg aaaaggacca aacagaaact
    53221 aaggatcaaa gcttatcgat tgagaatgaa tacacctata ttattgcttt gttgatgcaa
    53281 cagacactga acgaagttgc tccgaacctt actcttcatg aagttaaacc agatcaaatc
    53341 aatgcaagaa atgtcatctt agcaaacgtt caaaatgatc aaaaaaccta tatcaatcaa
    53401 gtagttattg aaacaaaaat tacaaaaaaa ggacattctg aagttttata tcaagaggaa
    53461 aaagaaggat tgcagattgc gccgaataca aatttttctt ttccaactgc tttaaatggt
    53521 caaccgctaa ccccaggtga atatcacctt acaatgactg ttttaggaaa tgaaaatgaa
    53581 aatggaaaat tttcgagaaa aaaaggaaat actacgatca actataccaa tcaatgggta
    53641 tttgaaaaag attttacaat agacggaaaa gtagccaagg aattgaacac gacggatgtt
    53701 acgattaaaa aagacaataa ttggatttat atcttagtag cttcactttt cttattgttt
    53761 attttattat gcatactttt gtttgttttg agaaaaaaag aagagaaaga aaagtaaggt
    53821 taagataaca aatagtaaat ttttattatt gtaggatggt tgggctcttt ttataacaat
    53881 ataaaatata ccattttatt aataaaaatg catactagta aatgatataa ttctaataac
    53941 aggtttgatc gcgagaaagg ccaaataaaa cgctctccaa aatgttaaaa aaaagacagg
    54001 acgatgtata ttatacaaac aagaatcgct gacaacagtg actcggatcc aggaaatcag
    54061 ttttatcccc agctagttgg tgaatgcatg gaaagtaata tacgtagaaa gagctgatga
    54121 ctaatgaatt atgagttttt tttaagcaaa gaagatcaca gaagttattc attacttaaa
    54181 tatttagaag caagctcaaa tttatcagag tcaatatcga atgttcaaga agagttgtcg
    54241 ctatcgacat ttctcttgaa aaaaacgatc gataagctga aacatgattt acaaaagttc
    54301 gatctagaga aaaatttgcg agtaaccgtc agtgaattgg atatatgtct ggaaattaat
    54361 ggaaaatgtt ctagcaaagc tctgttatct cgttatataa cggattcttt atctatgaaa
    54421 atggtattgg ctttctttca ggaaaaatat atctcagtag aaaattttgc taaacagcat
    54481 cacgtgagtt attctgtggc ttataaagta ttacaaagac taaaaagaaa tctaaaaaaa
    54541 tatcaaattt tttttgaaaa gagaaagttg
//
LOCUS       DAPBBG010000015        52614 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPBBG010000015 DAPBBG010000000
VERSION     DAPBBG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 52614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.015"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            241..825
                     /locus_tag="RD477_001018"
     CDS             241..825
                     /locus_tag="RD477_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633397.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            836..1081
                     /locus_tag="RD477_001019"
     CDS             836..1081
                     /locus_tag="RD477_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633398.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1087..1809
                     /locus_tag="RD477_001020"
     CDS             1087..1809
                     /locus_tag="RD477_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633399.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1940..2683
                     /locus_tag="RD477_001021"
     CDS             1940..2683
                     /locus_tag="RD477_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633400.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2689..3054
                     /locus_tag="RD477_001022"
     CDS             2689..3054
                     /locus_tag="RD477_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633401.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3070..3600
                     /locus_tag="RD477_001023"
     CDS             3070..3600
                     /locus_tag="RD477_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633402.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3762..4070
                     /locus_tag="RD477_001024"
     CDS             3762..4070
                     /locus_tag="RD477_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633403.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            4066..4485
                     /locus_tag="RD477_001025"
                     /pseudo
     CDS             4066..4485
                     /locus_tag="RD477_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4622..5422
                     /locus_tag="RD477_001026"
     CDS             4622..5422
                     /locus_tag="RD477_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633404.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            5459..5641
                     /locus_tag="RD477_001027"
     CDS             5459..5641
                     /locus_tag="RD477_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633405.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            5752..6069
                     /locus_tag="RD477_001028"
     CDS             5752..6069
                     /locus_tag="RD477_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633406.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            6072..6304
                     /locus_tag="RD477_001029"
                     /pseudo
     CDS             6072..6304
                     /locus_tag="RD477_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6419..6898)
                     /locus_tag="RD477_001030"
     CDS             complement(6419..6898)
                     /locus_tag="RD477_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633407.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7018..8355
                     /locus_tag="RD477_001031"
     CDS             7018..8355
                     /locus_tag="RD477_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDT7633408.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8378..8905
                     /locus_tag="RD477_001032"
     CDS             8378..8905
                     /locus_tag="RD477_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633409.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            8892..9455
                     /locus_tag="RD477_001033"
     CDS             8892..9455
                     /locus_tag="RD477_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633410.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            9877..10614
                     /locus_tag="RD477_001034"
     CDS             9877..10614
                     /locus_tag="RD477_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633411.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(10631..11047)
                     /locus_tag="RD477_001035"
     CDS             complement(10631..11047)
                     /locus_tag="RD477_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633412.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11440..12687
                     /locus_tag="RD477_001036"
     CDS             11440..12687
                     /locus_tag="RD477_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7633413.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            12955..15345
                     /locus_tag="RD477_001037"
     CDS             12955..15345
                     /locus_tag="RD477_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDT7633414.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15394..17229
                     /locus_tag="RD477_001038"
     CDS             15394..17229
                     /locus_tag="RD477_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7633415.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17298..18161
                     /locus_tag="RD477_001039"
     CDS             17298..18161
                     /locus_tag="RD477_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7633416.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            18151..19260
                     /locus_tag="RD477_001040"
     CDS             18151..19260
                     /locus_tag="RD477_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7633417.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            19599..20399
                     /locus_tag="RD477_001041"
     CDS             19599..20399
                     /locus_tag="RD477_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633418.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            20487..21293
                     /locus_tag="RD477_001042"
     CDS             20487..21293
                     /locus_tag="RD477_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633419.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            21525..22394
                     /locus_tag="RD477_001043"
     CDS             21525..22394
                     /locus_tag="RD477_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDT7633420.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            22414..22761
                     /locus_tag="RD477_001044"
     CDS             22414..22761
                     /locus_tag="RD477_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633421.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            22774..24036
                     /locus_tag="RD477_001045"
     CDS             22774..24036
                     /locus_tag="RD477_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633422.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            24116..25492
                     /locus_tag="RD477_001046"
     CDS             24116..25492
                     /locus_tag="RD477_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDT7633423.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            25489..26118
                     /locus_tag="RD477_001047"
     CDS             25489..26118
                     /locus_tag="RD477_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633424.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            26137..26616
                     /locus_tag="RD477_001048"
     CDS             26137..26616
                     /locus_tag="RD477_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633425.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            27118..27171
                     /locus_tag="RD477_001049"
                     /pseudo
     CDS             27118..27171
                     /locus_tag="RD477_001049"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27175..27774
                     /locus_tag="RD477_001050"
     CDS             27175..27774
                     /locus_tag="RD477_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDT7633426.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            27790..28266
                     /locus_tag="RD477_001051"
     CDS             27790..28266
                     /locus_tag="RD477_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633427.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            28283..29185
                     /locus_tag="RD477_001052"
     CDS             28283..29185
                     /locus_tag="RD477_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633428.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29258..29452
                     /locus_tag="RD477_001053"
     CDS             29258..29452
                     /locus_tag="RD477_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633429.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            29557..29985
                     /locus_tag="RD477_001054"
     CDS             29557..29985
                     /locus_tag="RD477_001054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633430.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            30220..30807
                     /locus_tag="RD477_001055"
     CDS             30220..30807
                     /locus_tag="RD477_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633431.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            30807..31706
                     /locus_tag="RD477_001056"
     CDS             30807..31706
                     /locus_tag="RD477_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDT7633432.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            31706..32620
                     /locus_tag="RD477_001057"
     CDS             31706..32620
                     /locus_tag="RD477_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633433.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            32672..32887
                     /locus_tag="RD477_001058"
     CDS             32672..32887
                     /locus_tag="RD477_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633434.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            33077..33331
                     /locus_tag="RD477_001059"
     CDS             33077..33331
                     /locus_tag="RD477_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633435.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            33510..34559
                     /locus_tag="RD477_001060"
     CDS             33510..34559
                     /locus_tag="RD477_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7633436.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            34736..34966
                     /locus_tag="RD477_001061"
     CDS             34736..34966
                     /locus_tag="RD477_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633437.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            34969..35226
                     /locus_tag="RD477_001062"
     CDS             34969..35226
                     /locus_tag="RD477_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633438.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            35443..36318
                     /locus_tag="RD477_001063"
     CDS             35443..36318
                     /locus_tag="RD477_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633439.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            36328..37122
                     /locus_tag="RD477_001064"
     CDS             36328..37122
                     /locus_tag="RD477_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633440.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESAKELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            37151..38269
                     /locus_tag="RD477_001065"
     CDS             37151..38269
                     /locus_tag="RD477_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633441.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            38302..38622
                     /locus_tag="RD477_001066"
     CDS             38302..38622
                     /locus_tag="RD477_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633442.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            38877..39566
                     /locus_tag="RD477_001067"
     CDS             38877..39566
                     /locus_tag="RD477_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDT7633443.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            39603..39770
                     /locus_tag="RD477_001068"
     CDS             39603..39770
                     /locus_tag="RD477_001068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633444.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            39790..41418
                     /locus_tag="RD477_001069"
     CDS             39790..41418
                     /locus_tag="RD477_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633445.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            41469..41840
                     /locus_tag="RD477_001070"
     CDS             41469..41840
                     /locus_tag="RD477_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633446.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            41925..42125
                     /locus_tag="RD477_001071"
     CDS             41925..42125
                     /locus_tag="RD477_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633447.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            42259..43485
                     /locus_tag="RD477_001072"
     CDS             42259..43485
                     /locus_tag="RD477_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633448.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            44335..45660
                     /locus_tag="RD477_001073"
     CDS             44335..45660
                     /locus_tag="RD477_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDT7633449.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            45653..46000
                     /locus_tag="RD477_001074"
     CDS             45653..46000
                     /locus_tag="RD477_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633450.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            46315..47172
                     /locus_tag="RD477_001075"
     CDS             46315..47172
                     /locus_tag="RD477_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDT7633451.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAERYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            47192..47752
                     /locus_tag="RD477_001076"
     CDS             47192..47752
                     /locus_tag="RD477_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDT7633452.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            48718..49485
                     /locus_tag="RD477_001077"
     CDS             48718..49485
                     /locus_tag="RD477_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDT7633453.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            49511..50056
                     /locus_tag="RD477_001078"
     CDS             49511..50056
                     /locus_tag="RD477_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633454.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            50057..50623
                     /locus_tag="RD477_001079"
     CDS             50057..50623
                     /locus_tag="RD477_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633455.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            50638..50886
                     /locus_tag="RD477_001080"
     CDS             50638..50886
                     /locus_tag="RD477_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633456.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            50906..51145
                     /locus_tag="RD477_001081"
     CDS             50906..51145
                     /locus_tag="RD477_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633457.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            51516..51797
                     /locus_tag="RD477_001082"
     CDS             51516..51797
                     /locus_tag="RD477_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDT7633458.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            51787..52128
                     /locus_tag="RD477_001083"
     CDS             51787..52128
                     /locus_tag="RD477_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7633459.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT        19985 a         7021 c        10514 g        15094 t
ORIGIN      
        1 taattgacag ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct
       61 aataatggaa taattctagg atttttaact ttttttattt catcatctaa tataaacata
      121 taaatctcct ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga
      181 catatttatg ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa
      241 atggatttga aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg
      301 gctgatattt ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat
      361 ttaatcgggt acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt
      421 gctaacaatc aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat
      481 gtgatccatg aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca
      541 atgacaacgg atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg
      601 cgtgatggtc aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa
      661 atttcttttt gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt
      721 aaaacagcct ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac
      781 ccatataatt ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa
      841 aaaaattata aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat
      901 gtattttact gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct
      961 attatttagt ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt
     1021 gttttattgg atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg
     1081 aataaaatga aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataacc
     1141 cttacagcat gtggtacaac taatagtgct aataataaca aagacacaaa gtctagtagt
     1201 acgatggtta ctaaaaacag cactacatcg gcatcaacaa caatgtcttc aatgactgaa
     1261 aataatgctg aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggatgaa
     1321 gtttataaca actttattaa tacagtaaaa aatgatgaaa attatgggaa tgatccattg
     1381 caaaataaaa atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca
     1441 gaatcaaaaa tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat
     1501 gatattacca taaataaacc agcttacgag tattacgcag ttgctgtaga tattactgat
     1561 ggtgtacctg agtgggtaca aaacattcct ggtctggctg atgagaacaa tcaatatcat
     1621 acgccaacag ttgcagtagt aacaccaaag aaagttaacg gcacactata ctgggatgta
     1681 atgggaatga caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg
     1741 gatttgggag catataaatt atatcaacaa tcagagcctg ttatggacga tcaatccaaa
     1801 gttaaataga tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa
     1861 tgatttctga aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga
     1921 ttataaagga gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa
     1981 gaatcaggtt ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact
     2041 taccggttac aataatggtg caaatgggaa gtggttaaag gcaacaagta ttcgtgaact
     2101 tagaagtgaa gtagaaaaaa ttaggcttgg tctcacaagt gaagaaatag ctaaactgaa
     2161 gggaaataat tcttttgtaa ttgaaaaaga aaaggaaaaa catctacttt atcgtgagct
     2221 gaaaattgaa gaatactatg aaattgattt acctgaaaaa ttaagaatta ttgatcaacg
     2281 ttcagaagaa aaatggaaaa ctgaaatata tccaagctta aatgactaca agacatcagg
     2341 aaagcccatg tataactaca caggcttttt aacctacaca caatacaaat atgcctttcg
     2401 caaattaaaa ggcattaaac aagctgaaat tgccctgtat gagatgaaaa aagtggatag
     2461 attgaaattg attcaagatt tagtgcaaaa tacatggaat caagcgattg tcgagctaga
     2521 aaaagaaaat gggaaaattt taactaataa atatctgatc gtaaatgaaa ttgaagaatt
     2581 aattggttta ttttcatgtg aaaaaggtga agtcttcttg aagtcatcac caacctattc
     2641 aattttggac agtccgtatc agtttataca ggcaaggaga tgagttagat gtctagcttg
     2701 aaagatgact atatccaagc aaaacaagag tatgaaaaaa tacagatgtt gaaatctgag
     2761 aaagtcgaat tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct
     2821 aaaactttag aggaatatga tcggcagttt aatcagatac atcacaatat cattttgctt
     2881 gttgagcagg caaagcgaag gtttgaaaaa gcggaatatg atctattaga tcaaggaatt
     2941 gtatgcttgg ataagggatt gcgaagatat tatggtgcag agttaaaaaa gcaaaatgta
     3001 gatagaaatg cactaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat
     3061 agaaagcaaa tgtcacaaaa cattagtgct gtaaaacaat taatagaaga agatttagag
     3121 accacagttc agagattagc ggaacgaatc cattttgcaa attatcaaac catctctaac
     3181 tggtcaagca ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga
     3241 aaagaatatg gtgttgatcc gataaaaatt ttcgagaaga ccatgattta ttactatcat
     3301 ggaacaccta acgaagtgtt agaacatctt ttggaagagg caaaagaaaa atcatcaata
     3361 ttagagctat cgcaaaatga caaagttatt caagtgaaat gcgttgatcc aacaggaaag
     3421 attattgtcg gttttgatat tgaaagaaaa tccaaaatag attttattac acaattaaaa
     3481 aagataaata atttagatat tctaacgctt agaccagatc atgaacagtt gtttatgagt
     3541 tttaaaaaac aattgaaaga aatacaaaaa agatataaca aggtgtttcc taatgcgtga
     3601 aaagctcaaa gaaatcagag ttttttggat aattaacaaa gaatgtatca tcttatatac
     3661 tcttggattt cttagcatga tcttgttttg gtggttatat ttaaaacagt aattggagag
     3721 ggtcaagagg atttttatca agataaggat gtggaaactg catgtggaat aatagattaa
     3781 aaacaggatt gttattaata gttatttcct gcgctatgat gattggtatg aggatacaaa
     3841 gggaacaatc ctattttgaa gtttctgcta acaacgtaat agaaaaatgc tattacggtc
     3901 aacattattg gagtgaggaa gtcagagaaa atatagatcg tgaatatgtt caaagaattg
     3961 tttgggatgc atattctatc aaagattatc ctaaaagttt aacgagtaga ttattttaca
     4021 gtgaaaaaga taatcaaaaa ctctcagatt tgatgatgaa aaaagtatga ctattattac
     4081 gaaagagaaa cagagtgcta tattcgaggg gaaaaagcct acaacaaaag tgactattgg
     4141 ggtgacaagg cgaagcgatt aactgcaaat tttagagtgt atgaatcaga gtttataaac
     4201 ttgacctttc ttaatagtgt ttttattgat tatgtaatta ctacgaaacg cattggaagt
     4261 tattcaagta cctacgcatc cttagttcca atgttaaact cgatgagccg tttcttaaaa
     4321 gaaagagaaa agaaagagct acagtatatc aaagaaatca tgccggattt tgaaatgcac
     4381 gaatggcaaa ttgctttatc agattggaaa ttggcgaata aggttcgtag aataacacct
     4441 tttcaagcca agcgcttttt gagagctatg aaatttgtgc aataaaaaaa gccaccagac
     4501 aagttgatgg agactaacac aattaccaac agttgtaatt gtgttagctt acaaattaat
     4561 tatggtgttt agtcaatccc aaattgtgaa atggcaccaa taattggaaa ggaaaaatga
     4621 aatgtctgaa gatttacgag agctgaagtt acgaaagaag cagtatgatc agtatttttc
     4681 cggtttgttg aataaacata ttgaagaagg acttgatcat gattttggaa aagatgaata
     4741 ttgtttatat tgcgaacgtt acaatgaaaa aatgtctgaa tatctacagc tgaaaaaaga
     4801 gatacagaaa ttagagaaaa aagtacatgc ttggaagtat aaagattcca tgtctggtta
     4861 tgttgaatct caaaataagc caagaaaaaa gaagcaagcg tatgttctaa agatggtaaa
     4921 tggggaagag ttaattgtat tagcgtacag tgaagaagag gttcgagaac tcgttgaaac
     4981 acctgtcagg aaaaaggttg aaacaataaa tgagatagac gacagccaat acaatagcat
     5041 ctatattgaa acaagtcatg gtggatataa gaaattagca actttcctta gaagttctaa
     5101 agttgggata atttctaaaa aagacataag tgctgttacc gatagttttt gtactttatc
     5161 tgttcctaaa gcttatttcc aatacgatag taaaagattt gaacgcttag agaaattctg
     5221 tagaccgtat actgtattga aaactaaaaa atttaataaa actaaaagag tttctataca
     5281 gttaccgact ggtcaaatct cattaggtga aaaagactat ctgataaaag taggaaaaga
     5341 gttaattata aaagtaccgg aacacttatt taaagaaaat tttgagatac gtgaaacctt
     5401 ggtatggaaa gaagaaaaat agtttggaag acatagaatg gtataagggg aaattgaaat
     5461 ggcagtaaat aaaatattgc aggtactaat catagacgaa tttggcggag gagaatggtt
     5521 tgatgtgcag tccatagact ttgaagaaga agtcgtagtt atagaaaata tttacggcga
     5581 agtatattca tattcgcttg aaggtgagaa tattgaggat ttacggttga ttggtgagta
     5641 gaaaaaaaca gaaatatcaa aatggagata aaaattaata gcatgtaaag atagtcatgc
     5701 ttgggttaaa aatgttaaaa ttcaaaaaaa tgagtcagaa gggataaaag tatgaaaaga
     5761 gaggaataca aacaaagatt aaacgaactg cttgaagagg acgaaacatt aactcatggt
     5821 tcgcctgatg aaattctata catgattgat aatatggtga tctttggtgg atatgaactt
     5881 ggtaataggt cagttgatca taatatttta gaatttgatg atgtatcatg ggaagaaatt
     5941 cttgattggg gaattttggc ggtacctgaa actaaaacct atattagtga cactatggtt
     6001 cctttttttg aggaattgga ttataaaaga cttcccaaaa atgaaaatca tatactggga
     6061 ggaaactaac aatgttaagt gacttattta ttttactgat ctatctaggt gtaagtgaaa
     6121 ttattgcgta ttttttgttt gtcattgcta attatggtat ttccagtgca atcgttcaaa
     6181 tgtttagatt accagtaagt gaactaaaaa aatgatgaga ttatcagcat atcttttaat
     6241 gtttttgaat gtggtcacgt taatctttgt agtaatcggt gtgactttcg atttaatgga
     6301 atagtgggaa atctataatg gaaaaaagag acacgaatga acatattttt tgaatcaata
     6361 ttgttgatgg tttttgcgat cagcgcttac cagaaatcta aaaaagagta atgattgttt
     6421 aatcatcact ctttttttgt agggaaattg aaatatgtgg agcttgtatt ggtttagtta
     6481 ggattgcttg gctataagga gctagttctt tatcaaaacc atcaaagtag gttttcagta
     6541 gcgatttaac tgatcgctca tcttgtttga tttggataat ttcagcatcc aaatcggttg
     6601 tgagatcagg agcttttgat gcgattacct gatttttttt atcttccaac tctcttaggc
     6661 tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat tccaacgtga gttgcaattt
     6721 taacagaagt ttcgtccgta ttgtcatcaa aagaactcat tggaatatca agtagtaacg
     6781 aggatgtttc ttttactgac tttttcttta cagcattttt tgtttttcca tcttttttgc
     6841 tctttactcg agatgttttt ggagctagtt taggtaaatc acttgtttta tcagtcattt
     6901 attagctttt cctttctatt aatttatgtt attatattat cataataaca taaaaaagta
     6961 cactttttgg gggcgtagtt attgaaaaaa tctaagagaa agattgccac attgcttatg
     7021 ttaatggtgc aatttacagg gattgcaatg agtgtaatca caataaatcc gcaagtgata
     7081 catgctgatg ggatagctac ttatgatgca caatttaact gtactactga aggtagtggg
     7141 tcaggaacag atagcgatgg aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc
     7201 aaaaatattg aagatattta caaatacatg aatggagaat atggcttatc aggtgctagt
     7261 attgcaggta ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg agcagtagaa
     7321 gggtattacg gagatggaca gagagaagtt gacttcatca aacaatttac tgatggtctg
     7381 atgggtattg gctatggtca atggacagct gggcgacata cagcattagt tgattggtct
     7441 aagaaaaata atggcggttc ttggtggact acagaagccc aattggactt tatgttcaaa
     7501 ggtgatgggg gattcgtagg aatattaaaa gattatgctt taaatgcttc tgatgatgtc
     7561 gtagaaaatg cagtaacttt tcataaaaac tgggaaattt cagctgattc cgaatcagct
     7621 gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt
     7681 atggacggtg caaaagatga aagtaagatc aataaaatat ccggaggttc aagtaccgga
     7741 gatggatcga ataattcatc aagttcagca tcaggaacag tagaagatgc ctgtgcaact
     7801 acggatgacg ctaattctgg ttctggatca attgggaaag aaggaaaaat taacggtaaa
     7861 aaaggtcaag taatagcgaa tatgacttat gaagaagctt tgaaaaaata tgggaaagcg
     7921 atcacgctac caaaattcaa agaaccagat tggtcaactt caccttttaa cggaatatat
     7981 caaggtcagt gtacagagtt gacgtgggct tatatgagcc aattatacga tgggaatcaa
     8041 ccgactactg gaaatggtgg ttatgtatgg gaaagctaca aatcggcagg agcaaaaata
     8101 acgaacaaac caatcgtagg ttatgggttt agtgcttcgg atggatggtt atgggctgtt
     8161 ggagatgcag ggcatactgg tgtagttata gcggtgtttg aagatggatc atttctatgt
     8221 gctaacttta atgttccacc aacccaagct ccaacaaggg gagtttcggt aacgttgatt
     8281 gatggagtgg acggatcaga taatgtgcat ttctttagcg gtgtaggaaa agctaaattt
     8341 gaaatcgaaa aataaattgg aatgaaggac gatatagatg aatagtaaag tgaaagatac
     8401 aattatcatt tctttgcttt ctggtatgtt attagcactg cttgttggaa tcctattctt
     8461 caataagcag aatcaggaca tagaagctga aaaacacagt gtgcatattg aagataagct
     8521 gacattcttt aaagaggtta aaccaaatga ggtggatgga ttacggaaag atttagaaaa
     8581 tgatattcaa gaaatgttga atggatcagt tcaaaataag tttgcagatc aatcaggtga
     8641 agaatttttg agaaaactat ttattgtaac aggtaaacca ccaattactg ataagagtag
     8701 tgagaaagaa aaaatcgagt actataagcc ttttactttt aagctaacaa actttggaat
     8761 taagacatct agtagtgggt tgtatgaagc attgaccgat tttgaagttc aatacaatgg
     8821 aaaaaaagtg tatgatacca cattaaccat cacactaaat aatgataaga aagtgactgg
     8881 gggtgctttt tatgcaaaat catgaaatca ctactgctaa aaaggcgatc ggaatacttt
     8941 ccataattgt gatcctttgt tgtgtggtac tatttcaaaa gaaaaatgct tatgataata
     9001 atgtcaaaga attggaagac cttaaaagta aacaaatcga agtagtcaag aataaccaga
     9061 aagtgaaagc tatgcaggaa gctcaataca aagaattggg aaatgctgaa attaaggaac
     9121 aaagtaggaa attctttaat ttattttaca actggaattc atgggaaaaa tatacgaaga
     9181 atatgaagga aatacaaaaa gaatttcctc agatagtaaa gtctagtcct gttgatattt
     9241 ctggcacgat ggtaggcact ggtaatagtc caatatctag ctatactgct gattactata
     9301 caacaaatca aaaaggtaaa atggttgaat tgattacaca gacaagaaat actccaacaa
     9361 cagcaacaac aacaatttgg cgtggagtaa caagcatagg agaaaatcaa ttgttttatt
     9421 tggaggattt agatagctat gtaagagcag agtaaaataa aaaaagagct aacggtacca
     9481 acgttagctc tttgtgctga cgatgataac aaaacctacc gaaaaaggat tgtttcatca
     9541 caaacacaaa cagaaaagaa ttgataaaaa acccgaacct cagaattttt attaatcaat
     9601 tcatttagat tataataaat tgagctgaac attacaatac gtatatgttg taaaacgtaa
     9661 aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa aactattaaa gacaatatat
     9721 atggtttatt tgacataaga aaatggtcta gatgataatt gtaatataga attgtagact
     9781 taaataaagc ataaaatacc ttttatgata aggtatagct cttaatttta agcagtttta
     9841 gatataagat tgaaagtaaa gtgggggaga ataataatgg cagatcataa attagaaaag
     9901 ttattagaag aaacgtggaa tccaaaagaa tttagtgaat tttttatgga gaattttgaa
     9961 actgacttag cagtgattgt taaagatgct cttagagagc aagggtatcc tgaaactgca
    10021 aactatataa acattaattt tactttgtat actgaaaata aaggaacttg ggatttttgg
    10081 gctactttag caaataagga gttaagtgat aaatcagaca caggaattcg taactttttt
    10141 gaatcaaaca gagatgatta tatgtatgct aacaatcaaa ataaattgaa ctttagagta
    10201 gagtttgatg aaacacctga agaatttata gaaagacaac cgcctaaaga aaatgtagca
    10261 aaggtattgg aagatcgttg gaattcagat gaaatagttt caacaattag cgaactaggc
    10321 ggacaatatg aaccgctagt agaagcggtt agagaagagc taagactgaa taagtttcct
    10381 gatgttcaaa atattgatgt ttctcaaatt gaaataaacg tcaagatcac gaataaactt
    10441 gattatggta gttgggcaga tatagcatta gaaaaataca tctactcaac gttaaaagag
    10501 tttatcgaaa atagaatgga tatcatgtat ctacagcatc cacagtattt aaactttggt
    10561 gttgaaatag ctacacctct tgaagaatgg aaaatggagc aagggcttga ctagcctttg
    10621 ctccattttt ttattttaca aatgaataca gttcgtcgtt tttaccaaga atgattgttt
    10681 gcccaacttt gagattgtca ggtgatcctt cagcggtttg taaggagaac gttccattga
    10741 acttatcgga taccgttata ggtagcttta attctgatcc ttcctcatct ttgagaattg
    10801 ctgtttttcg ttcatcaaaa taaacagtct ttattaaata atttttcgta gtgatctcag
    10861 cagatttgtc tttttttgta gtagtaccag tttctttaga attagaggta ttttctacga
    10921 ctattccatc tttggcattt gagcttgtgt ttatttgatt gaatattttc agcgacaaga
    10981 taacgctaag aatagtcaat atagaaacaa gtgtaattaa tactgataat ttcctttttt
    11041 tgttcatttt ttcttaacat ccttttttgt tttgattagt tagatattcc ttatctaaca
    11101 agccttttta ggctattaaa tgaagataaa ccccaaaaaa atattgcaat tatgacagtg
    11161 caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa tcgcaaacaa
    11221 aaagaaattg aacctagaca aatacaactt gtataccgca agggaagcaa gtttgatttt
    11281 gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc gtttgcctaa
    11341 aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag gaatagaagc
    11401 attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa gagaaattcg
    11461 cttggtatgg ttttattggc atcagctgta cttgcggggt tatcagtacc agtaacctca
    11521 catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac aggacaatca
    11581 agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct taaaagctta
    11641 ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt ggcaaaagga
    11701 gaacaacgga ctatcccgat tggacgaact ttcaacatca gtaaagatac attaacagtt
    11761 aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc tgatcctatt
    11821 gaaaatacgg agcaagagaa caaaaaatcc gagactacaa aagaatcgga accaacagca
    11881 aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag ttcatctaat
    11941 acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag tagcactacg
    12001 gaagctgata ctggaacatc aacaaataac tcacaatcaa catcaaatga tgcaaatgaa
    12061 tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga aactgacagt
    12121 tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt tccatttatt
    12181 agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt acattataaa
    12241 ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact atctcctgac
    12301 aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg aacttatcaa
    12361 ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa tgttgtagat
    12421 atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa tcctgtagta
    12481 gcgtgggtaa cttatgcctt tgaacctgct caacataaac caatgttttg gggcgatgct
    12541 atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta ccatgttgtc
    12601 gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc atataacgta
    12661 tctcaattag cggtttcggt acgttaataa aagatatcgt ttatatacta agtagttata
    12721 tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg tgggtagaac
    12781 taaaaggggt gatgctatga atactagagc gttgaaacag cttgctttca acgaaaaaaa
    12841 tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt accacaccga
    12901 catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg agagatgaag
    12961 catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac tttagtaatg
    13021 atgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga tgtcacaaaa
    13081 ccgacaagtg caaaatttat agaaactggc gtaaaaacag acggttatat tagggttaat
    13141 atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc tcatggaaat
    13201 attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg
    13261 aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca
    13321 aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta
    13381 gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac
    13441 cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct
    13501 aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac
    13561 gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag
    13621 ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa
    13681 attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca
    13741 ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact
    13801 tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag
    13861 gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa
    13921 gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga caataacaat
    13981 gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc
    14041 ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag
    14101 gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac gaacacaatt
    14161 aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct
    14221 gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga
    14281 cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca
    14341 actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga tggacaaact
    14401 tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca taaagtcgat
    14461 caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca
    14521 caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg
    14581 tatagcctag aagaaaccgg agtaccagta ggttacacag gtgcatttaa gcaagagggt
    14641 atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa
    14701 ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc
    14761 accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct
    14821 acgttcaaca ttattgcgaa caaaacttat aacttagaag aaacaaaagt accaacaggt
    14881 tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa
    14941 tatactgcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt ggatgatcaa
    15001 gggaaagcat tagcaaacgc agaattcact ttgacagacg acaaaggtgt aaaagttgta
    15061 caaaaaacgg acagcaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa
    15121 atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc
    15181 gttgctgacg atggacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca
    15241 gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg
    15301 agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga
    15361 aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat
    15421 ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc
    15481 cttggactag gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga
    15541 acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt
    15601 gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag
    15661 ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat
    15721 actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac
    15781 agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta
    15841 aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac agtcaaaggt
    15901 gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg atcagatgat
    15961 caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt aaactcagca
    16021 gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt tgaattgcga
    16081 aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac aggtaaatta
    16141 actgtggatg gtttggaatt aggtgactat tatttcctag aaaccgattt gggaacagat
    16201 gctaatagaa caacctattt actagatggt aaaaaattca aatttagcgt taaacgtggg
    16261 gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat tactaaggta
    16321 attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa accatcaatt
    16381 cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg tgcagagtta
    16441 acttatgatt catcagaagt gatcgaagga atgactttag atactgatta cagtatcgaa
    16501 cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa gcttcagaaa
    16561 caatttgatg caggggatcg cttctttaca attttgatga acgctcatac taatagtaaa
    16621 gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa tggatatgaa
    16681 cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca ccaattcatt
    16741 aaaacagacg gagatacaag caaagcattg agtggagcaa aatttgtagt gaagaacaaa
    16801 attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaagc tgttacttgg
    16861 attgcagata aatctaaagc tactgaattc gtttccggat cagacggtaa gttccaaatt
    16921 gaaggtttgc aatataccta tgatgtagat tttgataaac aaggtaatcc aacaatcaca
    16981 gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg atatacacca
    17041 actagaaacg atgttccatt tgtggtatcg aatgatgatg cttctagcga agtaacagac
    17101 atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac agtagtattt
    17161 ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg agaacaacac
    17221 aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc tttttaaaaa
    17281 gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc atcttaaaaa
    17341 ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag gcttatacct
    17401 actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa
    17461 aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga aaagacacag
    17521 aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat ggaaatcttc
    17581 agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt aatgtggatt
    17641 tgccaattta tgatggtgta aacgatttcc aactatcagc aggagcagga atattaaccg
    17701 gaactgatta tccaactggt gggattggaa accattctgt tatatcagct cattcaggca
    17761 ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat aaatttttta
    17821 ttgatatttc agggaaaaaa cttgcttatc aagtagatcg gataaaaaca attctaccaa
    17881 ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt atgacatgta
    17941 cacctgttgg agataattct cataggttac tcgttagagg ggttagcata ccttaccaaa
    18001 aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg cagatgaact
    18061 ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc attattaaaa
    18121 aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact ttttttgact
    18181 cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca tcaattcaaa
    18241 acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac tccttcaaaa
    18301 gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca aggattagac
    18361 aatactcaga aaacagataa aggagtagac aatactcagg aaacagacgg tggatcattt
    18421 gatattcgtg aagctgaagg aaaggtcaat ccatacacag gagatgtttt ttcaaaaaat
    18481 caaacgaaag attttgaacc atctacctat tcaggagcgg ttggttatgt ctatattcca
    18541 aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt
    18601 gtggcacaaa tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata
    18661 gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc
    18721 ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca
    18781 gacgttatta gaaattacgg tcagaattca acactgactg atattcctga tttagatatg
    18841 ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt
    18901 ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac
    18961 gctgatgcca ttgatagtga atccgtttca gataagccag aaactgttac atcttctaat
    19021 gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat
    19081 attccggaac aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca
    19141 atatttacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtatta
    19201 ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttag aaaagcataa
    19261 tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct
    19321 tttttatttt gaatagagct agattcactt ttaaataaaa taaaaactga tcttaattcc
    19381 aaagaaaaag tcaagaaaaa aatacaacaa atttttttat cagaatattt atcagaatat
    19441 tattagaaaa gataaactca aaagcttaat ttaatagggt tattggttgt atttagaaat
    19501 aatgtatgga aaagctataa agcaatagat aagatgtaga aaatcttatt tgtgaatgaa
    19561 aaacaagtca tcgtaaaata ttaataggag gtgtagtaat ggtggaaata tcaaaaacag
    19621 ctttagactc attagctaaa ctatttagta gcaggtttga tggtgcagaa tttgttgaat
    19681 atctaagcca aaacgaaagt ggaaaaactc tgatcgaaac actgaaaaaa gactatttga
    19741 atgaaaatga ctttccgaca tggacgaata ttgaagatgc tacatgggat gtagtgtgta
    19801 cgatatattc tccttgggta acgattgctg attttgtttt agataaaatt ggaggggata
    19861 cataccctga tgccaaatct cttgctacaa gtgactttaa aacgttctat ggttatctaa
    19921 tgggtctaat aggcaatgga atagatttga aagttacgcc aaaacaaaca cctgaagaaa
    19981 ttgaaaaaga aaaaggagag gttcaaatgg cttatgcaga tgacagagaa aaagtaaaat
    20041 tattggaagg taaattatct gaagaagaac ttggaaaaat ggtgcaagaa atcaatgaag
    20101 acagcaacta ttttcctgaa ttgaaggtat atcaaaacga cgaagatttc tttaatgaaa
    20161 tgtttagtaa tagtccatgg gaagcagtga gtaaaacaaa tttaggagaa tggaactttc
    20221 aagatgatct agttgcattt agcaatgatg ggtatttacg atcatattca gaaagtgagt
    20281 atagagctga attaaaaacg tatgaaacgg atattctgat gagctattta aatctattag
    20341 agagtggtca gggagagtta agagataaag ttgaagaact attaaaagaa gagctatagg
    20401 gattatttca acataaacac aagcagaaaa ctaccaaaca aaacgtacca tgcaaaaagc
    20461 cggttaaacc ggctttttgc tctattatga gaggagatca aaccatgagt gcaacaaccg
    20521 atatggcttt agaagcagta ataaaagata atacaggaaa tgtcgaaaat gtagaagact
    20581 ttagcgatat tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa gaaaatatcg
    20641 aatttgagat caagaatatg gatgacttaa aagcaaaatt tccttttatc gcatcgaaac
    20701 taagtcaatt tgccgaggag aaatcgtgga atgaattagt tagtttttcc gaaaaaattt
    20761 atgatattac gcaagaagat gagtgtgttt catgggaaat gaataatcaa tacaagaaaa
    20821 ttgatgcttt cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa ggtatagata
    20881 ccgcagataa ttggttaaat aaagaatcct ttattaaaag tatagcggat gatcttgaaa
    20941 aacaatggtc taaggaagac tatgcagagt acctaaaaac agaaagagat acagaggtga
    21001 aagaaaaact cactgaggta tgtttggatg aacatattga tcccaatatc gtaaaacaag
    21061 aagactatga agtggttatt gataaagttc ctactgattt tgaggaagtt gtaaagtata
    21121 tagttgaaga agcattatcg caatactctt cagatgagga agtggacgaa gaaactgttc
    21181 gtaccagagt ttggcaactg gtttttgatg acaaagatta taaatatttg agtgatcaaa
    21241 actcggttaa ttggagtgta cgattattaa ttgatcaaaa tgatattgaa taaaatagag
    21301 ctgaaaagct ctattttttt tgataaaaaa actagctatt tttttaaaaa taatttggtt
    21361 caaaatttat ttttttaact tgataatttt ttgccatcct tttttaaatt ggctatatat
    21421 caaggtttat agtggggata aataaaaata catttgacaa aatttacaat atagtttatt
    21481 atatatttat cttattatga taacacaata aaaggaatga tggtatgaaa aagaagaagt
    21541 ttagtcgatc gttgttagga attttagttt tagttgcggt tggtcttagt ggcaagggtt
    21601 actttcagaa taacagcact caggaacatg attttcagat caaaagtagt gttcaggtag
    21661 gtaataaagc agacagtgaa tcaaaagaat ttttagggat acgttatgat ccaaacaaat
    21721 atccaaataa tatcgtaaac attaatgatg ggaaatatac actgacaaaa gaagaccatg
    21781 aagcattatc agaaaaaggc agtcaaacct tttgggtaaa ttatgcagat ttagatcaac
    21841 ttggaagagc aggagatgtt gaagcgttga tcacctatga tgctataaat tctcactcat
    21901 ctaaaaatgt ggaacgtcca tctttcaaaa gttctgtcca tattgcagga gaatacaagg
    21961 atggagtatt tgatcaagag agagatactt gggtcggcaa agattcaaac aatgaaatca
    22021 ctcagctaga aggatataaa ggttatctct ataacaaaag tcattcatta gcatggtcgc
    22081 taggaggaga tatggaggta cacaatttaa cacttggaac gagagcgcaa aatgttggga
    22141 aaaatgatca gaatggaggg atggcttact cagaaacgat tgtacgtgat gcaatctata
    22201 aaaataagaa tgaaaaagta ttgtataaag ttgatccaat ttataaaggg aatgaattgg
    22261 ttcctcgtgg ttctcatgtc gaagctttct cagttaacga taatggaaaa acggtaaacc
    22321 ttaatgttta tgtatttaac aatcaaaaag gaatcaacat caactatcaa aatggtactt
    22381 gggtaagaga ttagaaattg agaaggtgaa gaattggaaa caataagcat caatggatta
    22441 gataaatcag ttgcaaatcg aataaaaaaa ggaaatggct actatcttct gaagaagggt
    22501 aaatattcag ttgctagaga gttagaaaca atgagtatcg tgtatgtagc taagaatcat
    22561 tttgacgtag aacaggctat ttttgatcca gattctgaaa tagggaaatg tgttgaatta
    22621 tcagagaaaa ctgttagtac aaaaggggcg atgatcattg agagagaaat gttagacgaa
    22681 atcagagtaa tcattcctaa agatacgcag atgtgtgagg acagcataag tgacgatgaa
    22741 ccaaaatcag aaagaaaata aggagatatt cagatgagcg aaaaatccat tgaattaaag
    22801 gaatataagc gaagcgatgg agaaaaatat tatcctgtag ccagtttttc taaatcagta
    22861 tatctggttg gacgtgcatt ggtagttggt gcaattggca tatatatttt gcattgggta
    22921 acaaaggttg tacaactgct tgtagatacc ttagtaagta ttcttgtctc gttaaatgat
    22981 gttaacggaa cagatgattt aggattaaaa gaaatgttta aagagttact caatgcgttt
    23041 tttcccaatt atttaagtta ttcatataat gtacaaagct tttttgcaaa gctacacttg
    23101 gtagacatct ctattttagt attagaagca ggaacggtat tctttgttgg atatttggtt
    23161 actttgatgt tcaggcatca ctatggagaa caaagaccat tttttgatga tatggaaagt
    23221 agaaggttga aaagagaagc tctagaagct atggatgtga agtatgatcc aaacaagatt
    23281 ataggagatg aaacgaacaa caaaaaaaga aaacgaaaca aaacgcaatt agaatccgat
    23341 gtgctatcaa cgcttaaaaa tatcaaaatt tctgttcata caagacgtga tctaagtgtc
    23401 aatgaagata ttcgagaata tatcgtaaaa atcccgcaac caaaagggaa gaaagttaga
    23461 gaaacagttt tacgacaaat tcaagattta gggtcgatcc tgacaagagc aacaagcgga
    23521 cgagccaaat ttggtgaaaa agaagatgaa gtttcaggcg attatttcat tttccgagca
    23581 tcagtaaata ttgaaagaga acgtaaggaa agagtaagta ccaaaaacaa agaaacggaa
    23641 agagcaacat cttcaatcgc agtttcaaat tcacaatttt cttttccaat caatctcttt
    23701 gccgataatt ccaaaaatat ccaaaaccaa accgaaaaag ctagaaaata tgccgaaact
    23761 caaaagcaat ctttagaact ttatctatct tctgctgacg tacctgcaag tctaacagat
    23821 atgcacgttg ggaatacatc tattgaatat ttgtttactt tatctcgtac cagaaaaatt
    23881 caactaagct caatgaaaga agaaattgaa acttacttgc atatatcagg tgtaatcgtg
    23941 cggatcaacg cagggcgtat tcaaattact ctaccattag gagaaaatga tcgtattcca
    24001 attgatgtac catcaatgat caatagcgtg ttttaaaaga catcacaaat agaaacagaa
    24061 aatacaaact accagttcac aaacagaaaa gtaccgagaa aagtgaggaa tagaaatggc
    24121 tatgaaatta ttagagaaag tcaaaaagag agccgaaaaa gctgaatcta ttttgctaga
    24181 taaagaacct aatataaagc ttaatggaaa attcagtgtc aatatgttga acacaaaggc
    24241 taaagacccg acagatgcta tttttggaat taagcctgat ggcacaattg ctcatttttg
    24301 catcagcgaa atgccacaca tgttggttgc aggaacgaca ggatcaggga aatcagtatt
    24361 actgaatgaa attcttgtta cagcaatgtg tcactcaacg cctgatgaat taaaaatagg
    24421 aattgtagac ccaaaaaggg ttgaatttgg aagatacaaa aaattgccgt tcatgttggc
    24481 tgatccaatt acagatatgg atgaagctta tgattttttt gaatatcttg tgatcttgat
    24541 gcatgaacgt tacaaactga tggaaaaagc agggttacaa aacatcacgc tatataacga
    24601 atatgccgaa aaaaatggat tagatagatt tccctatgtg atcttattag ttgatgaata
    24661 ttcacagttg gttggaacac ataaagaagt agaaggtttg attgttcaat taggacaaat
    24721 ggcaagggca gcaggtattc atgttatctt agctacacaa tctcctcgat caacagttgt
    24781 cactggtatt atcaaagcca attttccgtc acgtgtttgc cttatggtag caagtgattt
    24841 agaaagtagg atcgtattag atgaaggcgg tggagaaagc ctaagtccaa agggagatat
    24901 gattattaag ctcgttaatg gaagtatggt tagagcgcag ggagcttaca tcagtaacaa
    24961 agaaattgaa acaattttca atcaccttag aaatactatg ccagaaccag aatatgttga
    25021 ttaccgagca atcgtagctg aatctcgtgg tgagggtgga gaagacgagg attttggtgg
    25081 aattggtaaa gctcctgtaa gaaaatctgc taaaagaaat ttgaacacac ctttagcaaa
    25141 ttcatcatct aaagagccat caattgaaaa gaagagtacg tttgctctaa aacttgaaaa
    25201 aaacaaagaa gaaaaagaaa aacaaggagc taaaacacta gaaaaacttc aagccgaaaa
    25261 attagaaaaa gttttaaaag cattagataa agctgattct gaaccgcaaa cctcaaacaa
    25321 cacgaataat gaacagccga tttcatctaa aactaaaccg aaaactactg atccgaaagt
    25381 ttcagtaaac gtgaccatca caaccgataa gtctgagaag aaacaagtaa acggaacgac
    25441 ttcacctatc atgcaaaaaa tgttgaaagc caaaaaacag gtgcataaat gatcattgtc
    25501 agttcttttt tgaggtggct tacgccatat aaatatacag atgaagtagt tgtgaaacga
    25561 acgaagaaaa gagataaaaa agcggtacta actcctaaac agatgcttga tcatggcatt
    25621 ttaggggtga aggttttaga agtgaataaa ccgtttgaat caaatagtgc aagagaaaag
    25681 ttgcttggag ctgaagccga tgtcgcatat ttttaccatg acaaaagcaa gcatcaaata
    25741 gctattaaat tcaatccaaa atcggcatgg cggtatcaag caagagatga cttgaaacgt
    25801 aggaaactta gaccaatgct ctatccagaa ggaaaagaag catttgatag aacccacatc
    25861 gttcccattg gttttcatgg tacagaatca gataaccgcc tattagtagg ttggaacagc
    25921 aatcaaaata gagttcagat caataaattt gagaagaaag taaggaaatt caacgatcga
    25981 aaaacgatcc tatggtttgc agatatttgt aaacagccgg atggaacagc aaagtgggaa
    26041 gcggtgatat ttgacgaaaa tggtaaaaaa gtaatgtctg aaacatggca tgataaagct
    26101 aaatttgttt ggcagtagca gtagaggtga aaaagattgc tagaactttt tgaagcaaat
    26161 agaacaggct ttaatcaaaa gttagaagtg ggagaaacta tagaatataa agataaaaac
    26221 tatattatta tcggaatcta taacacgaga attaacagaa taggtgatcc acgtataacc
    26281 agtgacttgg tttgtcaatc tgttaactat tcacctgatc tgacaagcag gtataagaaa
    26341 tacgtgttat tagaatatag acacaagatt actgatgaaa ttggtgtaaa caacacacga
    26401 aacatattta agatcggaac agtgattcct tatagtaaca acgaagaaaa aatattttat
    26461 cagatttacg gaatagaaaa gtttgaatat gaacatgtcg atctgctagt gacgtaccaa
    26521 atgcgtttaa tagaaccttg gtcacaggca gaaattgata aagcagtaaa attgaaccga
    26581 ttatcaaaat ttaacgtatt aggaaatgcc ttttaaaaat cgcaatacac aaacagaaaa
    26641 ataccaaaaa attagcaatg aacgtaggta agaacatgac aataaataat aataacccta
    26701 aaaaatataa tgctcttgtc caatgctata tcgaaaacaa aattacaatg caagaagtcg
    26761 ctgatgaatt aggcaaatca aagagcctag tatcacacga ttttaagcac aatgtttttc
    26821 cgaacccgat tgagctgagg aaaaaatttc aaaagagagt ttatcaagaa ataggtaagt
    26881 ggattgatga agggaatgca attgaccctt taattgaaaa atatcctttt ttaaaaagca
    26941 aaaataaaat tcaaaggtta attcgtcaag gcgatattca agctaaagaa cctgttttga
    27001 caaccaatga tttggctaaa ataaatcttc ataaacaaat tattgaagac tttgaaaaaa
    27061 atctttcaac aactgaaatt ttaaaaaaat acaatattcc gagaggaaag ctaacatcga
    27121 ctctttttag agaatatgga gaatcggcag ttactaaagc aaaggaagta gcgaatgaga
    27181 gaaaaaaaca aaaatagtag tcgataccta gtagcgactg tagtttttgt ttgtgtattg
    27241 attttcctgt ttgatttttt gcagattgga aaaagcataa ttggaaatac attgagtgca
    27301 aaggaaatgg ttgctttagg tggtttgtat gaaggacact tgttcccgaa tatattcatt
    27361 tctatcgttt ctcatattaa cttgtaccat ttaatttcta atttattgtt cttagtagtt
    27421 cttgaagaag cactgaatga tcagtatgag tggttaacat tattgatcgt tttaatcata
    27481 tcaggatttc taggaagctt agttcctttt ctgctacaca gcaacatcgt aagtatgggg
    27541 cttagtggag gtatttttgg atggattgct ttatttgcga ttgcatacat ccgttttgat
    27601 agcaagaaac atttattact aagtgttttt gtattattga taatcggcat tttactggta
    27661 tctacttttg aatcaggcga gacgaacgtc ttgtcacatg taatcggatt tgtctgtggg
    27721 atgatcttgg gatttattct aaagaacgtt tcattgatca aacaaaagaa ttaaaaggga
    27781 gtgctaaaaa tgccaaaaaa aaaagaaaag aaaattccag tgtttttgtc tgtctcagaa
    27841 atgttatcac ttcaaatact gttaagaaaa aagattgaaa caatagacga agaagttaga
    27901 aataaaaggg ctatgtacga acaacgtgaa gcgctaagag aacgaatcca ctattttgat
    27961 gatatacaca ctaaaatcta tgaagcacgt acaaatcacg aagacgaaag caaattagga
    28021 ttagctcaac aacgaatatt tgaatggctg aatacgaata ctgagcaaga accgttaaga
    28081 gcaattagta atctttttga atttggaacg aagcattggt ttaacgctgt agaaaaggat
    28141 gctgacttac aagaagcgtt cgatacacta agcgaggata gcaaatccat gcgagactta
    28201 ttgatcactt atctaaatga gcatgaattt gaacagacga aagttaaaaa gaaaaataaa
    28261 aaatagaaag ttgtgatgta tcatgctgtt taccttatta ggttatttat gtttagttct
    28321 tgcttttttt ggagcaattg ctatatttgt cggtcagttt caagaaaata atccgataat
    28381 gcgtaacata ggcattggaa ttgttatcat atccttgatt gtatttggtg ccacaaagat
    28441 ttttgggagt aatgatcagc agtttggtat ttctggagtt gataatagtt atgctccaga
    28501 ttcattggga agagtagtga tagatggaac gtttgagaat gtaacgagct acaaaaatgt
    28561 ttcattgaca gtgaaagaag atgacaagaa agcatcagat gacgttctcg ctacgatcaa
    28621 gccgtctcaa tcacagtttg aagtgtttta tacagtcaaa gagaataacc ctgtacgcaa
    28681 gctaacatta gtttttaacg ttgacggaga agaatactat aaagatgtaa aaatcaataa
    28741 agcatcaacg aaaaagattg tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac
    28801 aactgaatca agttctactg tcgaagttga actaccagat aaaattgatt ttgatcctga
    28861 aaaagtcaaa tcgtttgaat ctgtttctgg ttctatggca gatcggttta acactgaagt
    28921 tttcaaagct atcaaaactg ttaaattgga tactgaaaag caaaggatta atgttacctt
    28981 attcaatgca gttgaatcta tggatcagga taaaaaagtt acggttgcaa gagaactagg
    29041 gaaaagagta attggattgt atcgaggtac ggcaaaacca gattcagacg gttcagattt
    29101 gccagttcac gtagtttatg aagacggtaa agtttttgga gactctacag caaatgaagc
    29161 actaacgatt aaattagcta aatagccgga aaattataat aaacaagtgg tgtcacacca
    29221 ccatcgcata ggtgtgacac cttggagatg attactgatg gtagtaaata aagaaaaagt
    29281 aagaaagtat gttactttaa aaatagcttc tgttgaatat ttagaaaagc gagctaaaga
    29341 agaagaaaaa acaagaggta agaaggtaac agtttcagag ttgttagaag aattaattga
    29401 gagtgaccaa acaataagac gtgcatttac caataggtct gttaaaagtt agggtgatac
    29461 tatctttaat ttcaaccaaa tttctaatat atgatgaata gtttccttgt acaaaatatc
    29521 taattaagag ataaaagtta ggaggattca ttcaaaatga ttatccaaaa gtttgactgg
    29581 aaaatgaaat taggaattag tcttgtaata ttgggattta ttattttttt ggggataaaa
    29641 gcacaaaaaa atcaatctac ttttgaaact atgtcttctc aaattattga ggaatgttcc
    29701 aaaatttatt attgtgagaa caaaactgaa gaatctaatg gcttttatta tatcgatcaa
    29761 ccacatgatg ttgttataaa aatggagtca catgaagtaa acgaatggat tcaaagaagt
    29821 actaatgtat caaaaaagtt agaaagatat tctcaaacgt ttttaagtaa agtcattttt
    29881 tcaaattgca aaaatgaaga aacgtctcaa agagtagcta gttctttaga aaatttaaaa
    29941 ttttctgttt caaaaaaaga gaaatttgta gatttacgaa aataaactat cgaaagtagt
    30001 atgattatga tcttaaaata tcatcttaag agaagaatta cgattgttta taggatttac
    30061 aaaatgaata agtttgaaga gtgatttggt cgcagactag atcactcttt ttgtgtaaaa
    30121 aaactttaca cttatttttg agtgtgttaa gattttttat atagagtgac ggtttcttgt
    30181 cagagtggat gataaccatc aatattggag gaataataag tggaaaacaa taatgatatt
    30241 agatatttta gcattgggga aagagttaca acagtaagtg aattatcaaa ggattataga
    30301 gataaagaag gaacgattat ttctcttaaa aagaatcgaa gaggtgtttg gcaatatgtt
    30361 gttgatttag gggatggcgt tacagttgag tgtaaagata gtcaactgat cagaaatgat
    30421 ggcagtcatt ccattgaaaa agcaattctt gtattcccaa aagtatcaag caagattatg
    30481 aatgactggt tgcttggaaa atttggtgag tacaataaaa aagaaatcgc agaagtgatc
    30541 ggcaaatctt atccaacagt attagggtat ctaaaaaaga taacgaacaa agaagcgaaa
    30601 gttgaggatg ttttaacagt aagagaactt cagtcattaa tcgaatatcg gaataaacaa
    30661 ataacaaatc ctgagtacct taaaaaagaa gatggtctat tgaaacaatt ggaacaggta
    30721 aataaaatcc ggaaaatgca aaaggtagct cctttaagcc tagataactt taaaaaaatc
    30781 tatttgaaag agatcgaaaa agactaatgt ggagtgtcac tttaccgttt caacctgtat
    30841 ctgcaccaag accaagtgca agaaggaatg ttgctgatgc aactaatgac gtagcagaga
    30901 aaaggatttc aacctattat gataaaaagt atctcgatta tttgaaaaaa ataaaaagct
    30961 ttatcgaaga tcaaggtttg ttagatgatg aattttactc gatcgtaaat gatccgatgg
    31021 gtgcaattat ggaagtggac ttttactatc agataccaaa aagctataaa aaagtctata
    31081 acattatgaa aacgacagcg ccagatttag ataatttagt aaaaagtgct atggatggta
    31141 ttttcaacat ctcagctata cgagatagcc atatcacagg gttaattgct tttaaataca
    31201 atgtggcaaa cgaaggaaaa acagttgtga ggatcaagcg atattcagaa tttgagtggg
    31261 atacttctga aggattgaac ggagatattt gggaagccac atttgatttc aaaccagtag
    31321 caacgccaag accaaagtct aaatttagag gaaatgggat tatcacttac tatccaaaag
    31381 agtataacga ctatttggaa aatatcaaga atacgcttaa agagaaagat ttggtaaatg
    31441 atcaactgaa aaaggtaatg aatgctccta tgggggttat agctcagata gattattttt
    31501 accaagtgag aaaagataag aaaaagcttg attcactgat gcgtacttca cagccggata
    31561 ttgataactt agtcaaagga acattggacg gaatttttaa caaggaaatt ggcatcaaag
    31621 atagcagggt ggtaggttta atagctttta aatacaatac ttttgaaaag tcgaaaacaa
    31681 acgtgcgact acaggagatg ggctgatgaa taataacgaa aaaattcttc atgtacttga
    31741 tagctttgaa acaattcaag aggaactaaa gaaatatcga gatgttttgg aacaaagata
    31801 tgattttgtc aatcagcaaa agagtaatca catggatttc attctcaata tgaatgactt
    31861 gaagaagaaa ttagttgaac gaaaagaaca agaaaagttg atcaaagctt actttgaact
    31921 cggtgaaaaa gaagttaaaa aggcaatgga actgaatgaa gatcgcaggg ttttagatca
    31981 gctgttagaa caattattgg tgatgtttca aaaaggacga atagatgagg atttaattga
    32041 agaagggttg agaaagtatc ctgcaaactc aggtatcgga atagtgctga aagcgattga
    32101 tgaagaagaa attgaagatt tcataccggc agaggatttt gaatcggcaa tggaatatat
    32161 aaaatattac agccaaggga tcactgcttt tagagagttt gatcctgaag atgttattca
    32221 tgacttgaac aacctaaaag aatggtgtga aggttatgaa gttgatgatt ccggtttgga
    32281 ctacttgatc agtattatgg aaatcgaaga agagatgccg gataagcctg atccaacaga
    32341 tattttagaa ttaatccatg atgctagaaa tccaattgca tacatttcta gaggatatac
    32401 tgtattggaa tactataagc cttatatatc agcgatgaac catttaagac gtgtattaag
    32461 ggaaaaacgt gaataccgct cagtgttaaa tgcatcgaat agattagaaa aagctgtttc
    32521 agagctagat gcttattatc gtgaacatta cctgcaagca ggtggtatgc caagaaatac
    32581 gaaagcaaat atcagtaggt atataaaaga agctgagtag gcggagttag cccataaaat
    32641 ggcaaaaggt aacctgaaag aggcgttttg aatgaaaagg gatgtttttg aattgtacgt
    32701 attagaacgt ctagaagacg gcatgatggc acaagagata attgatgaaa ttgatgatat
    32761 gatcagcaga tttggtcaat tggaattgaa tgatggaaac aaaaattcgg tagtgctaca
    32821 acaattttgg ttaaataatt tttcgtataa ggattttatc aaagaaaagg caaatagatt
    32881 gtactgacta aattcacgag gatcagtgat gagagcttat tgctaagagc agttaggctc
    32941 ttttttgttt gtgttaaatt attttatttt acaatacaca tttatacaca aaaaatgttg
    33001 acggatagta cacaaatgtg gtaggatata tttgtaagca agttagatta acaagttaaa
    33061 ggaaggaaga gcagttatga aaaaagaaga actaatagat atgtttcaaa ttgttgaacg
    33121 agcaaataat atgggaatta tgttttttga tcgtatttct ttaaagatgg atttaagcgt
    33181 tgcccaccaa gaatttaatc tacgattaaa agctttattg atttctgatg atgttaattt
    33241 cgctcatgat gttgtaggta tacagaacca tatagataga gaaaacaaac gtatgggtga
    33301 tggattctta ccaagatatt caagtttatg atttggcgaa agattcaaga gtatgtgtca
    33361 ttattgatag gtactcttga attggaaagg aggaatgaat ttaagacaac aacgattatg
    33421 atcttaaaac aatcgtttat gtaattggca atcttacgaa taattttcat gcaataatga
    33481 caataaaaaa tgaaaagggg taaaatcaaa tgaaaaaatt tttagtagga attctattca
    33541 caatggggct agtgctatta tcaccttcag tttcagcaga aagctctatt tccatgtatc
    33601 gtatgtataa tcccaatagc ggagagcatc tatacactag aagcgttgga gaaagagaca
    33661 atctgaaacg ttatggatgg cattacgaag gtgtcgcgtg gcaagctcca acaagtggtt
    33721 ccccagttta tcgcttgtac aacagatata acggtgaaca tttttacacc gttgatgccg
    33781 gagaaagaga taagatttcg cacacaggat ggacgtacga aggtgttgcg ttctattcct
    33841 acaacggagc aaatagagtt cctgtgacac gcttgtacaa caaatacgtg aattggcacc
    33901 attacacgtt agatcaaaat gaaaagaacg taattagtaa acaaggttgg aagattgaag
    33961 gtacgggttg gtatgcaatg ccaaacagcg caccaaatcc tacacctgca cctgctccaa
    34021 gtaatgaaaa agtgttaaat gttcctgtca tttggcaagg taatacgatg ttgtgtgaag
    34081 gtgcttccct gttagaggga ttgcactaca aaggagtagc gacaaatcaa aacttaacca
    34141 gttttgtaaa ctatatgcca agagctaatg acaataatcc atatcatggc tattctggcg
    34201 aatggcgcta caacgtaaat ggtacgtatc aaggcatgat gcctgatcca gtagttaaat
    34261 gggcaaaaaa tgttggtggt aatgccgtta atatcactgg ttcaggaacg aatgggatca
    34321 agaatgaaat tcgcaatggt aatccggttg taacttggat tacttataac tatgcttcac
    34381 ctcaattcaa acaaatgcct tggggacgtg cagtctggaa tggacatgtg gttctcgtgg
    34441 atggcttcaa aaatggtgca taccatgttg ttgaccctgt atttggcgca aaatggatca
    34501 atgcaagcac gtatgagaga gcatttaatg tgactggaat ggcggttgct gtacgttaat
    34561 tgatagaatt aataaataaa tgaaataaga gcttaattac tgaaatggta aatgggctct
    34621 tattttttag aaaaaaactg ttttttgtaa tacgaattta taacaaaaaa cgttgaatgt
    34681 agtacacaaa agtggtatat taaaaccgta gagattatgc gaagaggtga ttttgatgaa
    34741 ccaaagaaga agaaaaaggc tagaagaaat aagcgagcga ctatccgagt taaaggatga
    34801 gttagaagta attcaagaag aagaggaaga gtacaaagaa aatatacctg aaaatctaca
    34861 gggatcagaa agatatgaaa aagcagaaga atccgttgat caacttgaag aagctgttga
    34921 tggtttatcc gacatattag aattgattga agatatagct agctagagat gattaatgaa
    34981 gatttcagga gtgagcaaaa aatgattgat atttcactga tagagaagct acttttttca
    35041 aataagtaca cagccaaaga gttggctaca agatggcaaa tatccgaatc agctattaaa
    35101 gcctatcgtc aaaataaggg aagcgtagga tggcgtgatt ggaaaggaat cagcattgga
    35161 aaagcagaag acataatgaa acgtgaagga tttcaagctg acaatgattc taaaaaagtt
    35221 gaatagttta atagtcgcca agaaaagtac caggacgagg ggaaaggtag attacaaagg
    35281 aaaacacttt taaataaact tcgcaaaagc atcaggttat gagtggctag ggctatacga
    35341 atgtgatgaa aacaaagggc tttaatgtca aagaaaacgc aatagggggg gaacaaaatt
    35401 tttaaaaaag atatttatgt ttggtttaat ggaaaaaaat atatgactac taacaaaagg
    35461 tggtcagagc tagtacaaaa tacagttaaa aaagtcatgc cagatggata tcatttcaac
    35521 tatatacaat gctcacaaac aactgaaagt atttattttt caatattaaa tgagaataga
    35581 ctctattatt tcagggtaag ttcacaccat aggaaaaaga gagaagataa tgttacgaca
    35641 tttttatcgg gaaaatacaa tggcttcaaa gagttaagtg aagccattgg tgagtatcta
    35701 aaaagtaata gatttgtcag aatttctgac aaacatatat tgatcttgtt actttatttg
    35761 aataatagct tcaaattgtt ttggagagac gacactttct actatattga gaacggcatg
    35821 gatatttatt ctggtatcaa cggcatatta aaacattata ttaaggcgtt acttcgatat
    35881 ggggttcttt atcaagaaga tttaagtaac caaattaaga ttagtaattc tggaaagtca
    35941 ttactggcaa aatataaaaa attgctttct cctatctaca aaggaataga tatggaagct
    36001 gtaaattctg aaactttatt taatttagtt gttgatgaaa agtttgcgaa tgaatttttt
    36061 aaaagtaaaa aaattgaaag actaaataac ctgtttcctg aaaatgcaat aaaagcttat
    36121 gtagataata ataaatcacg attatacaac tggtttattc ccaaacggtt gcgaggaaaa
    36181 atcaaaattg gcgatcaagt gaaagtaatt aatctaaatc atactaagat cgttaccgta
    36241 acggagttgt atatagctga aaaagaagat attacaaaaa tgaaaccagt tattaaaata
    36301 ctagataaca aagagtgagt ggaatatatg gtagcaaaaa agaatactct aaaagaagct
    36361 tctgaaaaat atcaagatat gaaaagacga ggattaatac caaaggacaa aaaaggtata
    36421 tggagagaat ttgttaagga agtcagtagt gatcgaagtg gcggtgactt tgttggacga
    36481 tatacagcaa aaggttatag caggcgctta aggatcactg ttgaatatgg aacgaatgaa
    36541 tcagctaaag agctgaagca aaggtactta gatacacgaa gagaagcttc aaaaaaatat
    36601 gatgatttca agagcagagg tctaattcct aagaagaaaa aggggatttg gcgtgagttt
    36661 ttcaaagaag aagatagcaa tcggaataag ggagacttca ttggacgata cacagcaaaa
    36721 ggctacacta agaatttagg gatcaaggtt gaatacggaa ctggtgaaag ttcatgggaa
    36781 ttgagaaatc gctatttaaa tacattgtta gagaaatata aagcagaatt attggagttg
    36841 ttcgatgtta atgattttat cgagatggct gaaaagtatg gtcatgaaga aggttttatg
    36901 atcaatgaca ttatggttat tgataatttt acggatttat tagcacttgc gaaaaaagca
    36961 agggatttaa gagaaacgca gatgtatcta ttcagaaata aaaacggaag aaaccggaga
    37021 actgaatcga gtgagattga agattactat gatgccttta cacacgatct gggagccttt
    37081 gtagacgaga taagtggtgt aatgaacaaa tatagggtct agaataaaaa agccggagat
    37141 gagcagataa atgggaaaca atagcatata cacacctgaa atgcagaata aaattaaaag
    37201 acttgtcgga ttagggtgga tgacaagagg aaaattagat tcttttagta cgatcaaata
    37261 tcttttgatg gacatttacc gtcagaacaa gcaaacagga atacattttt tgggctatga
    37321 taagccggaa gatgcacctt tctttgttcc aagttcagcc tatagagcca gtacctccgg
    37381 tagcatgtat aacttggatt tgtttgagga aggagcaaag atgatcgaat atgatttcaa
    37441 cgaagcctat tcgaggatca tgagaaatta tcctttgccg tcaaacactt atttgaaagc
    37501 aaaatataat acagatcaag ctattttgag agcgataaat agaccaaaag taagaccgta
    37561 tgaaaattta aaaacgtata tgttcttttt ggttaatttt gaaggaacag ccaaagaagg
    37621 aacatatgct aatccaaaat cacagtcgat gtttgcggat tatggcgaaa atcccaaaac
    37681 agctaatgag cctatttggt tatcggagat tgagctaaca cttatccttg attattatga
    37741 tattaaagag atggaagtcc tagatagcca cacatttttg acgaaaaaag gattactcaa
    37801 agaatatttt gaaagaatcg agccattaaa aactgatcct gatacgttga agctttataa
    37861 aaatcttaga acgaaagtat ttggtgctat cggacaaaaa tcattaagag aaggagaaac
    37921 aagcaccttc gataaataca gtatgtataa tcgagcgtat tcctccatgg tagcaggcgt
    37981 atttagagat cgtatggtta gatatgaaca gaaatacgtt catagcgcat atgggctatt
    38041 aaaaattcgt actgatggat tgtatttcag atccgaagta ccagagtttg aagagctgta
    38101 taaacttgga gtagttaaga aaaaagttca tacaatatcc aaagaagaag tcgaaaaagc
    38161 tttagaattt aaaaacaaag gatttgaaaa taagggctcc tttgggaaat ctaaagatga
    38221 agttaagggt gttgtgatag acgatgattt cgagtttgat tttgactaaa tactctttgg
    38281 aaaagatggt aggaggtaaa tatgaagaag aaaattgtgg aaggaaatgt aggtgttatt
    38341 attctaaaag attcaactga ctatgctaaa tcaatggcac taaaagacga aaaagttgca
    38401 cgcatggata aagatttagt tgaatttctg atggatgata cagaaagatt aaaaggggat
    38461 cagtttttga gtttaatgaa agacaaaggc ttaaacacat atggtttatt tgattataag
    38521 aaattagcta tagagtgggt aaaagaagga gaaatatttt ttattgaaga atgggaaggt
    38581 actgagaact tagttcttca tcagtatctt ttgaaagctt aatctaagta aaacagttct
    38641 gactagttat gcttgtcaga atttcaaagg aagttttcca taaccatcat cattcaaaaa
    38701 taaaggattt gaaaataagg gttcctttgg gaaatctaaa gatgaagtta agggtgttgt
    38761 gatagacgat gatttcaagt ttgattttga ctagattttt ttgcaatgtt taagagtaaa
    38821 caaggtaaga actaagatca tgaagattga gagtaaagaa gatagaggac gaatgtatga
    38881 gaacacacta tatttattta gaagataaaa ttgtagatga aattccggca aaaagtcgag
    38941 aacaagctag aaaattattt attgaaaaat acaaagagat caatggagat agtgaagttc
    39001 caaaagtggt cgtaacagtt agaaaaaggc aaacaaaaaa gagaagtacg tttcatgtca
    39061 gccctgatat atataatctt atagagcgac atagaagtga agataatatt tctatctctc
    39121 aatatattgc tacagttatt gatgaattga tttcaggaaa gtatgactat gcttttgatt
    39181 ttgagggtga aaaggattac aaaagtgttg ctgtctttca atctgatttt gagaagctaa
    39241 aaaactatgc tggcaggaga aatgttaatt ttgcaagact actagaagag ttctactccg
    39301 aagagttggg agtaggaaat gaatacaaga gagaaactta tgcgaaattg tctcataagt
    39361 tatcccaagc gcttaaagaa gtgacagaag ttaaagcgga actagatgct aaacttgaag
    39421 aagtgaaaaa atatcaaaca gagaggaaga acaacatgaa gaatcaagtg gaatttgacg
    39481 aacagaaaga atttgaaaaa gcaagaaaac gctataacaa aattatgaat gaacttagct
    39541 atgaacactt aacagttcat gaataattga gtgagggtac agaaaaatat acgatcgcag
    39601 atatggttaa tgaagtggaa actcttttag cttctttctt tgaaaatggt cacgtaaacg
    39661 atgaatggcg aaaagaagaa cctaaaaggg ttaacgctta tattgctcga ttaaaaaggt
    39721 ttattgataa atataaggag aatagtgaca aattttctaa agataaataa ggagagagtg
    39781 tgattacatt tgccagaaat cgaaggaata ttttcgccaa aaataggtaa aaaacagtta
    39841 gatttcagca gttttccgag cgtaaataga gattataatc taggtgatga ggtacagata
    39901 ctaaatattg atttcaaaga actagacttc gctgttgctg tttccaacaa acccaaggca
    39961 ttggaacgag tgaaaaaaat ataccaagag aatgatcaaa atatcaaaaa tatgatcaaa
    40021 aagtatagac gagataattc aaaagttgaa gacgttttat tcaacggtac ggcaaaacat
    40081 gatttgaatg tttatatggc attaggagtg gctttgtact gtaattcaat taatcagaca
    40141 gatattttgg ttaatatcgt gaggaatagc tatcaaaaaa tattaaaaat aagtagcgaa
    40201 acaaaccctt attatgcaat gaaatatctt ggggataatt tattaaatga taaaaaattg
    40261 atgcacaaca ctgaagagct gttatctaat ttggctgcga ttaactttct attcaaaaca
    40321 ggttctgaag attattttat agataacgta ctaaaaggtg cttacggaac agcattttat
    40381 ccgctttaca atatgaaaca gcataaagcc gaacaaaagg ataacaaact tgaaattaaa
    40441 aagatgaaag aactattagg tataggattt gtaaaaccca attcaaaagt cactattgct
    40501 tcgttagcaa ctactaacaa tgatgggaaa atagtaaatg ggtttgacga ttcagatata
    40561 gatatattgt caaaatcata tttttctttg tatggagatt ttgcagattt gatgggtatt
    40621 gatagttact tagtcaataa ttttgagatc aagggtagcg agctgaatga tattgtgtat
    40681 ttcgttttaa tctctctata tgcaattgat cagaataccg agtaccgaca aattgactat
    40741 ttgctagttt tttatagttt gttatatcag ttcgttttaa ctcggttgtt taaaaatgct
    40801 aaggaagaat tgatcctgaa ttctgattat gaaatagcaa aacaaattgc aagtattaga
    40861 aaagaaaaca aatctgaagt aatcaaatta agaaaagaat taagtattgc gagtaaagaa
    40921 aacggtcggt tagaatcttt agtcaaatcg gcaaaaaaag aagaggaact tttgaagcaa
    40981 gagattaaag aattaaaagg aatcagaaaa gaacttgaac agctgaggtt tgaaaataaa
    41041 gtattaaagc aattgcagga gcaagaacat agacaatcat taaatggtaa aactgaagag
    41101 ccttcactgt ctgaaatgat agcttatcta aaacataaaa acattctttt agtgggagga
    41161 catgtaaatt ttcaaaataa attaactgaa ctattaccaa atattaaaat gtttgatgta
    41221 agcgaaaata atggaaagat acttaaaaat ttgaatacgt gtgatgcgat ttttctatat
    41281 acagattact tgaatcatgg tctatattat cgtgttatga gtgaaatttc taataaagat
    41341 aagatatttt atttggataa tcatgtgaat atgaacttaa caattaaacg tatttacgat
    41401 cttttatcac ttatataaag agagaagtga taaataggac acaaaatttg gaaagtaggt
    41461 aagtcactat gagagaacta actttatctt ctaaggaatt agaaaagaaa actgctttat
    41521 taaacgaaat atttagtgct gattgtataa tccaaagttt aaaagaaagt tcattttcaa
    41581 aactaacaga tcaggaaaaa atttcttata tgctaagttt agataatcta ggaactaaat
    41641 atgatgaatc agcaagtagc gataagcttt tgattgaatt agtatcaagt cttagtagtt
    41701 ttggaaaaat gaaaagattt aatgtgatat tagaaatttt tgcttggtta atcccttcat
    41761 ttatttggtt tttttatatt ttcatagttg aatctgagtt aatagccaag gtctctcttg
    41821 gactgttaat tccgatataa ataggttcag caatttactt taataacttg tatacacgta
    41881 ttcaaataaa aaagcagtta ttacatgaag gattaagtgg taatatgtta aaaagaattg
    41941 catgggataa agataataaa acagatattg agcttaaaga atataaatac aattatttct
    42001 cgagatgtat agatagaaag ttactgttta aaaaagaagg attagaattt agttttgatg
    42061 aagatgaatt tgaaaaatta gtgatggact ggcgtaagaa tttactggat acggtacaat
    42121 tctaaaagca attgaggtcg aaattccgat acttgaatca tgatctatat tatcgtgtta
    42181 tgagtgaaat ttctaataaa gataagatat ttcatttgga cagtcatgtg aatatgagct
    42241 taacaattaa acgtatttat gatcttttat atgtttataa acattaatga ggtgctaaaa
    42301 ttgacaacaa taactatgac aaaaaaaata gaagtatatg ttgatggatc acaagaagaa
    42361 agggattcct tttttgattt tttttggaaa aatagtagag aatatgtaaa agcgttgaat
    42421 ctggtatata aacagctaat gtttgaagag ttgataaaag atgcaattaa agaaatggat
    42481 caaggttatg ctgagagaga agaaagtatt caaaatcgga ttgaaaagtt agcatatact
    42541 gatccaatag atcaaaaaaa acttgataaa gcaaaagctg aattatctaa attgagaaat
    42601 gaaaaaaata aagaagcgag agaaatatta acgcaagcga tagggttgaa gagtcaaacg
    42661 agaacacgag atgtagcaaa aaaattagac tttgactttg gagattttgt tgattccgca
    42721 aatatgaaag ctagtaaaga ttttggcaat gattttatcg gaattgtgac cggacaaaga
    42781 acgcctagaa tatataaaaa tagaaacaaa tatggattac taccttttag aggtcgagat
    42841 attaaaatcc ataaagatga taagttttat atttatttac ctaaaggctt taggtttaaa
    42901 gtgaatttag gtagcaagcc aagaaaagca agagatgttg cttcagtttt acaacacatt
    42961 atggatggcg attacaaatt aaatcaaagt aatcttattt ttgatggaag aaaattattt
    43021 tttaatctaa caatttcttt tgaaaaaagc tataatgaaa tggaattcaa gaaaggtaat
    43081 gaattgatcg taaactttgc agatttgtgt agcggtgaat gtttaattaa tgataaatct
    43141 gtaatgtctt ttggtgatag ccaatatata agaaacttca aaagtaaaat tttagcaatg
    43201 aaatcaaaag aacaaagccg aggagtcttt tctaagggcg gtcatggtcg aaaaagaaaa
    43261 ttagatcaag atcgatggga agcaataaag aaaagagaaa gtaattttgt taaaacttat
    43321 aataatcaat gttctgcaaa aatcgttaaa actgctattc gcaataaagc tcaaattatt
    43381 cgtatcattg aaccagataa ggcttatggc gaatgggctt actatcaatt gaaagaacaa
    43441 ctagtaaaca aaggaaaaag atatgggata gaagtcagaa gttagtaaat taaacaatcg
    43501 ttgatttggc aacgaaatag tgagggaaaa tccactcact gaagggtaaa ctccgtcaag
    43561 ttgtgtgacc agacggaagg acgaatgttg ttgaggtttt agaaccatat aaaacagcat
    43621 gtttctcaaa tttttaggat aagggaaatt gctagcagtt tgttttagaa ccatataaaa
    43681 cagcatgttt ctcaaatgag tactttaaat taattgatgg cgttcctgct ttagaaccat
    43741 ataaaacaac atggatttaa aacaagaagt aagtcagttc tccttttact ttagtaagga
    43801 aaaataaagt attctcaata aaacttaaaa taagcaaacc tttgttttaa tagctaatcg
    43861 aagtaaaacc caaagccata tgaacccctg aatcagcgaa agtttatgat gatttggatt
    43921 actaatagtt taggaattag atggtaaatc taaattaaac aatcgttgat ttggcaacga
    43981 aatagtgagg gaaaatccac tcactgaagg gtaaactccg tcaagttatg tgaccagacg
    44041 gaaggacgaa tgttgttgag gttttagaac catataaaac agcatagttc tcaaattgtt
    44101 taaaatgatt gtcaagttaa aaaaatctgg gatcatataa aatagtatag ttctcaaata
    44161 tgattagaaa ccctattaaa gagcaatatt aagccaactt acttaaatcg tttaattaag
    44221 aacaataatc tcttttagtc agacttttag tctgactttt ttgtatttcc aaaatttatt
    44281 ctttacaaaa acgaacatac gtttgtataa ttgcttgtga atgggggaat aaaaatgttt
    44341 gattacagta gagaaccggt gaatgactat tttttaatcg acatgaagtc tttctatgct
    44401 agcgtggaat gtattgaacg aaatttagac ccaatgacta caccgttaat tgtcatgagt
    44461 agatcggaca acactggttc aggtctgact ctagcttctt cacctactgc aaagaaaaaa
    44521 tatcatatta ctaatgttag tagagctact gatttacctt atccattacc aaaagaactt
    44581 catattgttc caccacgaat gaacttatat ataaaaaaaa atatggaagt taatcagatt
    44641 tttagaaagt atgtcgcaga tgaagattta ttgatttatt cgattgatga atcaatttta
    44701 aaagtgacaa aatccttaaa tttatttact agttcaggaa caagaagcga gagaagaaga
    44761 gaattagcta aaataatcaa agatgagatt cagaaagaaa caggtctaat agcaacagtc
    44821 ggaataggag ataacccatt attagctaaa cttgcactgg acaatgaagc gaagagtagt
    44881 aaagaattta tagctgagtg gacttatgag actgtacctg aaaaaatatg gaaaataaac
    44941 gagatgactg acttttgggg cattggatca cgcatgaaaa aaagattgaa tcatatggga
    45001 attatgagta tagaagattt agcaaattgg aatccctaca caattaaaag tcagttaggt
    45061 gtaatcggac tccagttata tttccatacc aatggaattg atcgtacgga tattgctatt
    45121 ccaccagaac caaccaaaga aaagtcttat gggaattcac aagttctacc aaaagattat
    45181 acgaggagag aagaagtaga actagtagtc aaggagatgg cagagcaagt agccattcga
    45241 attagacaac acaattgcaa aactggttgt gtacgcttag caattggtac gtcaattttg
    45301 gaagaaaaat caggtttctc gcaccagatg aaaataccaa ttacagataa tacgaaagaa
    45361 ttacaaaatt attgtctttt tttattcaat aagttttatg aggggcagga agtacgacat
    45421 ataggagtaa cttatagcag attagtatac acagattccc tacaattaga tttatttagt
    45481 gatccaagaa aaaagattgc tgaagaaaat ttggatagga ttattgataa gattagacaa
    45541 aaatatggat ttacagctat tatacatgct tcatctatgt tagaaggtgc aagaagtatt
    45601 acgagaagca ctttaatagg aggacacgca ggaggattag gaggtttgca taatgattga
    45661 atataacgat agaggacgtt taaaatggtc gggattttat ttgtctgatc atactgaaag
    45721 aatcaatgca gacaatgtac aacgtgctaa tcataatttg gcaaaagaac aaatgacgac
    45781 tgaagagatt gcgaaaatac tgagaactgc tgtcataaaa aatagacccg tctgcattca
    45841 aaaagaggaa cgaaacgctg aaggttacta tccagatgat gtagtaggaa aaattattgg
    45901 atatgacgag cttggtgtat ttgtggaaga agaaaaagtt cactatgatg aaattaggaa
    45961 tgtttctttc tcggaattat taaaatggag tcagatatga acattattaa aaaatacgaa
    46021 ttcggttata ttacgtatga acagctaata gaggaattat ggggatatgg tcaacaatta
    46081 attaatgaag tggggattga ttgcttttgt ttctatattg aatccggagc aggataccat
    46141 aagaacagat actatatatt tccatatcca ccaaaatagt ctcttagata tttttaaatt
    46201 ttctccaaat actttcaatt attgtgaaat atttcccttt aattaatcaa attttttata
    46261 aaagtaatat ataaaataaa ttgcttgaag tattatattt agatatcata atttatggaa
    46321 gaaaaattaa ttaaaaagga tgagattata atggggttac tggttccgag agctaatact
    46381 tatactatac ctttaacgaa acgccaatta attgctgaaa gatatcaaag gattactaga
    46441 gccatcaata gagagttttg gaattctgaa agtgatactg ctcacagttt atacgtaggt
    46501 tcttatggga gaggaacggc aattagtact agtgatatag atataatagt tgaattaccg
    46561 atggcagaat ttgacagatt caaaaattat ttatcaaatg gcccgtctaa gttattacaa
    46621 gttataaaaa acgcatttca ggaaatacta ccaaatagtg atattagagc tgatggacaa
    46681 gttgttaaaa taaatttcca tgatggaatc aaatttgaaa tagttccagc ttttaatgaa
    46741 aaagattatt ggggtgagtc aaaagggttt atttatccag attcaaacat gggagggaac
    46801 tggaaagcta caaatcccaa aaaagagcaa gaagccatga aattaaagaa tacaaaaagt
    46861 aataatttat tatatgctac gtgtaaacat tttcgacatg tacgagatac agaatttacg
    46921 agttatcatc tttcaggaat agtaattgac agtttcgtat atgaggctat gggaaattgg
    46981 aaattcgttg aaaataacag tgggggtcaa aacattagtt ctgtatctta tgagacagct
    47041 ttattagaat actataactc acataaagtt atgggcggtt taaatttata ctctcctgga
    47101 agcaatcaat ttgttaattc tgatagtagt atcatatgtt tagaaaaagt attaaaaaaa
    47161 atagctttat aaagttttat ggaggaacaa catgggagac gaaaaaaagc gcttggaagt
    47221 tcaattacgt gaagcatatg gacgagtaac gtatacttac accactcatt taaagcaagc
    47281 cgataatatt atttctgaca ataaaatttt aaggtgtctt caaattggtt tgtcagccct
    47341 tactactgga ggggtactta gtaccattat tactgataat agtgagttaa cctttgttgc
    47401 tggattatgt tcaacaattt ctcttggatt aaacttatac tttaagaatt ttgatcttgc
    47461 tgaagaagct aaaaatcatc aaattgttgc cgataaatta tggttgatca gagaagaata
    47521 cgtaagctta ctaacagatt taaatcgttt aagtgttgaa gatattgtga aaaaaagaga
    47581 tcaattaatg aacgaaactc atgaagcata taaaaatgcc cctaaaacaa atagtaaaag
    47641 ctatgaatca gctcagaaag cattaaaatc tgaagaagag caatttttta cagaagagga
    47701 attggataag atgttaccag agcatcttag aaaaaagagt tctaaatatt gaactctttt
    47761 tttatgctta aaattctctt gtaatttttc tattcatcta tatttaagaa gaaatatgat
    47821 gatagaaaca tctcctaaag ccaccgcaag ccgttctaag catagcttag cccgacattg
    47881 ctagatatgc tcatctcacc atgaacgttt cctaaagcca ccgcaagccg tcctagccat
    47941 tgcctagtcc gacatcgcta gattagtgca tctcgccata aaagttttct aaagccaccg
    48001 caagccgtcc tagccattgc ctagtccgac atcgctagat tagtgcatct cgccataaaa
    48061 gttttctaaa gccaccgcaa gccgttctaa gcatagctta gcccgacatt gctagatatg
    48121 ctcatctcac catgaacgtt tcctaaagcc accgcaaacc gtcctagcca tcgcctagtc
    48181 cgattttgct tgatcggttc catttctgtt tatttagtgg gtcaacaccg taatcttaaa
    48241 tagccatcgc ctatttaaga ttactaggtc tgctttgaag gtaaaaagtc tttcattctt
    48301 caagactttt aggctttcaa gctacgcctg aaagcattga aattcatcgc tctttatttg
    48361 gttttgccat ttataattcc ttacgcccat tatctttttc gcctgtcata ccttgtcaga
    48421 agtgtttaga gcttatctca tctttggatt aatacaagtg gcgtaaattt ttttctaaac
    48481 gcaagcgtta cgaaaataat ttacttaaac acttgtatta atccggcaga taagaacgca
    48541 ttctaatcac tcccgacaag gcaagacagg cgaaaaagat aaataaatga acgaaaggaa
    48601 gaaacataaa atgcaaaacc aaatagcgat gaagaaaaaa ccacctccct ctaaaacaat
    48661 ccaaacaaaa aagaattttg aaaaaccaaa aatcaaaaac agaaagaagc gttcaacatg
    48721 agagatagaa ttttagaagt taatcaagaa atggtaaaca agttaacaaa tatcagtggg
    48781 aactatgaaa agtacgatgg tactttactt gataactata tttttcacga tacacaaaat
    48841 atcagaattg gcagaagtaa accgagagcg ttcattctca tcaaagagaa atatttgaat
    48901 gaatggaata gcacctatga aatggttatg actgataact ttgaaaaggt attggatttc
    48961 agaaatgctt tgttgggttc tgatatggga aatagcagtc ttgaatctat tgaagttgaa
    49021 aacaagaccg agaatgaagg tgaaggattg attaagaatg tcttgaaaat cgaacaaaaa
    49081 tatatcagtg aagatagaag tttttataga gtcacaagct cggttggtct agcaaacttt
    49141 gctactaact taattggaaa caatgctagt gaatctttgc tagtgatgtg tctgaataca
    49201 aagaatgagg tcattgctta tagcgaagtt ttcaaaggtg gaattaatca atcagtagcg
    49261 ataccaagag agatttttca actagctttg ctaaacaatt cagcaaaaat cgcagtatca
    49321 cataatcatc cgagcggata tgaatttcca agtgaaaatg acaaaagttt tactacgaat
    49381 ttgaaaaacg caggaaaaac aattggtatt gaattgttag accatatcat cgtaacaagt
    49441 aaaaacacaa gctattactc atttcaagaa aatggttctt tataaaaatt agagaaaaga
    49501 ggaaaacaag atgggcgaaa gaacacaagc gttaattaca gtcaaatatg gtaacagcaa
    49561 aacgaaagca agcgtgtcga ttcattatca gtgggggtat ggtcgatata tgctgatgga
    49621 cgtattgaat caaggaattt atttaatcaa taaagcatac gacttacgag ataaatcaag
    49681 agaagaatta gcaaaaggaa tctttgatag aagtacagga gttatcaatt ttgttaccta
    49741 tgaatctgga gtgagtgctt atgatgaaac agggtttggt gggcgctatg aaccttatca
    49801 agaaacgatt gaagatgtga tgaagttttc agataacaat gatggctatg ctcatttaga
    49861 gatggtgttc gatgaaaatg gatttagtct agatgataaa accagattgc gattgtttga
    49921 ttttgagggt caacagatag gactaaacaa gttttgtgaa agcggaaaag agtttgctac
    49981 gaaagatttt attaagggat acagattatt gctagaaaat tatggaataa acctagaaga
    50041 agagagcgtg aaataagtgt ttgaaaaaag aaaagaccag ttccctaatt tagaagcgaa
    50101 aagtcaaaaa gtgattgatg gtgaaatgat tgaattattc caaaaaagtt atggaaccat
    50161 agaaactacc gcaagttgca aatactactt tcaaggaaag agaaacacgt ttaaaggctt
    50221 tgccgattct gatgaagaat tggcaaagaa aatcattgat tggcttgtca gcagtgaaca
    50281 cgtagcaagg gaagataccg ttagaacaac gattaatggg gaagtcctaa caggtactag
    50341 atgtgtttgt tgtaatcatg aatttgtaga agatgaaggt tgctaccgaa tcaatgaata
    50401 tggtgatttt atttgccaga gttgccacga gatagaattt ccaaccgaaa gaagttggtt
    50461 tgaatacatg ctacaagatt atgagatgga aaaagaaaat ggtgaagtag ttttttgtac
    50521 caaagaggaa ttgagtaaga gaagcgatga tgaaattgaa gattggtgtg tgtggttagt
    50581 aggggcagat gattgcgttg catattacac aacagcagaa taggagtgtg aatcagcatg
    50641 gaaggaatgg attttctaga tcatgaagat ttggttgatt ttggttatac atggaaagga
    50701 atggtaggta ttagcagaag tcttgctaat gccttttacg aaagaaatta tgcagtatat
    50761 gttctttatg atgacgatac agaaagcctt gttgacgagg aatataaatt ggatttagag
    50821 aatgttttat atggaataga aaaggaagat ttagcaaaat atattttttc atggttgggt
    50881 caataagtga aaggaagata gaaacatgac aaaatttaaa agtaagggta tgcaatggtg
    50941 ttcttattat tatgttggaa atgatgaaga tttattgaga aaaacccaac gaaaaggtta
    51001 tccagataag atattgaaac aaataggtta ttacatgcca gaagagagga tagaaaaaga
    51061 attttgtgag gttctttttg atagtgaaca gctattagtt ttgttaaccc aaaataaatt
    51121 gatttgccaa tttgaaaata attaagatga ggtggtgaac catgaatgaa gataagatgg
    51181 tattaagtga atatgctaga ttgagaacta ttaattcttt agcatctcat ttaagttgtt
    51241 ctttcctaac aaaggtgcta aaattaattt acaaatgtga agattttgta caagagaagc
    51301 acaaagacaa gcctactgcg aataggcttg tctttttttc attcattacg attccgtatg
    51361 tgtgggttag cgacaatttg ttcaattacc gtatgatata gcatcttaat ttgattagat
    51421 tacttgcaat gtagtttatt aagagtataa tataatcata aatgtggaca atttgataaa
    51481 taatgagtgt tttatactca aaaggagtgg atgaaatgaa ggtcaaaaat ccaactgaaa
    51541 aaagcagaat ccaagtcgga atagacaaga atttaaaaga gaacgcagaa atgatattgg
    51601 aagagctagg tttaaatcct acaacagcaa taacaattct ttacaaacaa gtagtcgcta
    51661 gaggtgagtt tccagtagag ataaagctaa gtgaagagga gaagcaagga atcagattgc
    51721 aacagctaac taaggacatg ccagtagatg ttctagatac agatgaaaag ttagaagagt
    51781 ggttcgatga agcctaatga gatagtaacg gtatatgttg catttacaga taaaaatggt
    51841 ggtaaaagaa gacctatttt ggtggttagt gataaagaag atagagtaga attctttgga
    51901 atcacaagtc aatatgagaa aaaatcagac caaataaaga gggtgtattt tcctattgca
    51961 gaatgggaaa aagcaggact taaaaagcaa tcatggattg acgtagggtc tttaaaggct
    52021 attcccaaaa gccgagagaa catatcattc aaaaaggttg gtatgttatc agctagtgat
    52081 acgaaaaagc taaataggtt tatcgagaaa ttacaaagaa aagtctaaca aggcttttct
    52141 tttttgttta tatgtatatt aagatatacg tttatataaa cgtatttatg tatatgaaaa
    52201 tatacgtaaa tatatacgtg gttataaaca ttaataaaac gttaagaatt gttaaataat
    52261 gatatttagt aatatacgaa ttataatata tattttatat aaaatgtatg taaataaata
    52321 aaatgtctga cggatattga atagcaataa ataacaataa ttcagacgga tacggtgagt
    52381 gaagtagctt gctacgaacg aacaggcaga atgaaacggc agtttcatta gagcaagtcg
    52441 cttgcgacgt agcgttaccg ctgatgtaac tggcaagtta catagagaga gtttgcttgc
    52501 aaacgaacgt tacctatttg aatagaaata aaaaataaca gattagtgaa ttttgaatag
    52561 gagccacaac tcaaccaacc gcttgcggtg ggtgagtccc acggaggaaa agac
//
LOCUS       DAPBBG010000016        49213 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPBBG010000016 DAPBBG010000000
VERSION     DAPBBG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 49213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.016"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(12..1052)
                     /locus_tag="RD477_001084"
     CDS             complement(12..1052)
                     /locus_tag="RD477_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7633460.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1329..3131)
                     /locus_tag="RD477_001085"
     CDS             complement(1329..3131)
                     /locus_tag="RD477_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633461.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3145..3897)
                     /locus_tag="RD477_001086"
     CDS             complement(3145..3897)
                     /locus_tag="RD477_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633462.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3906..4268)
                     /locus_tag="RD477_001087"
     CDS             complement(3906..4268)
                     /locus_tag="RD477_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7633463.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4444..5316)
                     /locus_tag="RD477_001088"
     CDS             complement(4444..5316)
                     /locus_tag="RD477_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDT7633464.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5342..5854)
                     /locus_tag="RD477_001089"
     CDS             complement(5342..5854)
                     /locus_tag="RD477_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDT7633465.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(5992..6783)
                     /locus_tag="RD477_001090"
     CDS             complement(5992..6783)
                     /locus_tag="RD477_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDT7633466.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6838..7446)
                     /locus_tag="RD477_001091"
     CDS             complement(6838..7446)
                     /locus_tag="RD477_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDT7633467.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7439..7756)
                     /locus_tag="RD477_001092"
     CDS             complement(7439..7756)
                     /locus_tag="RD477_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7633468.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7912..8640)
                     /locus_tag="RD477_001093"
     CDS             complement(7912..8640)
                     /locus_tag="RD477_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDT7633469.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8799..9680
                     /locus_tag="RD477_001094"
     CDS             8799..9680
                     /locus_tag="RD477_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7633470.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9917..10960)
                     /locus_tag="RD477_001095"
     CDS             complement(9917..10960)
                     /locus_tag="RD477_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7633471.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10963..11631)
                     /locus_tag="RD477_001096"
     CDS             complement(10963..11631)
                     /locus_tag="RD477_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7633472.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11773..14109
                     /locus_tag="RD477_001097"
     CDS             11773..14109
                     /locus_tag="RD477_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7633473.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14224..14535)
                     /locus_tag="RD477_001098"
     CDS             complement(14224..14535)
                     /locus_tag="RD477_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633474.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14716..16191
                     /locus_tag="RD477_001099"
     CDS             14716..16191
                     /locus_tag="RD477_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDT7633475.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16238..16822
                     /locus_tag="RD477_001100"
     CDS             16238..16822
                     /locus_tag="RD477_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDT7633476.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16824..17243
                     /locus_tag="RD477_001101"
     CDS             16824..17243
                     /locus_tag="RD477_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDT7633477.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17524..17808)
                     /locus_tag="RD477_001102"
     CDS             complement(17524..17808)
                     /locus_tag="RD477_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDT7633478.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18161..18871)
                     /locus_tag="RD477_001103"
     CDS             complement(18161..18871)
                     /locus_tag="RD477_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDT7633479.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18868..20340)
                     /locus_tag="RD477_001104"
     CDS             complement(18868..20340)
                     /locus_tag="RD477_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7633480.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20478..20951
                     /locus_tag="RD477_001105"
     CDS             20478..20951
                     /locus_tag="RD477_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7633481.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21104..21655)
                     /locus_tag="RD477_001106"
     CDS             complement(21104..21655)
                     /locus_tag="RD477_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7633482.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21760..22290
                     /locus_tag="RD477_001107"
     CDS             21760..22290
                     /locus_tag="RD477_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDT7633483.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22773..23216)
                     /locus_tag="RD477_001108"
     CDS             complement(22773..23216)
                     /locus_tag="RD477_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7633484.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIISFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23232..24392)
                     /locus_tag="RD477_001109"
     CDS             complement(23232..24392)
                     /locus_tag="RD477_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7633485.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24899..26599
                     /locus_tag="RD477_001110"
     CDS             24899..26599
                     /locus_tag="RD477_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633486.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26701..27534)
                     /locus_tag="RD477_001111"
     CDS             complement(26701..27534)
                     /locus_tag="RD477_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDT7633487.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27838..30003)
                     /locus_tag="RD477_001112"
     CDS             complement(27838..30003)
                     /locus_tag="RD477_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7633488.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30263..32557
                     /locus_tag="RD477_001113"
     CDS             30263..32557
                     /locus_tag="RD477_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDT7633489.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32550..33215
                     /gene="pgmB"
                     /locus_tag="RD477_001114"
     CDS             32550..33215
                     /gene="pgmB"
                     /locus_tag="RD477_001114"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDT7633490.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33208..34215
                     /locus_tag="RD477_001115"
     CDS             33208..34215
                     /locus_tag="RD477_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7633491.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34237..35256
                     /locus_tag="RD477_001116"
     CDS             34237..35256
                     /locus_tag="RD477_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633492.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35537..36904)
                     /gene="nhaC"
                     /locus_tag="RD477_001117"
     CDS             complement(35537..36904)
                     /gene="nhaC"
                     /locus_tag="RD477_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDT7633493.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37013..38434)
                     /locus_tag="RD477_001118"
     CDS             complement(37013..38434)
                     /locus_tag="RD477_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7633494.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38677..40554)
                     /gene="tdc"
                     /locus_tag="RD477_001119"
     CDS             complement(38677..40554)
                     /gene="tdc"
                     /locus_tag="RD477_001119"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDT7633495.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40845..42101)
                     /locus_tag="RD477_001120"
     CDS             complement(40845..42101)
                     /locus_tag="RD477_001120"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDT7633496.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42213..42418)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42681..43982
                     /locus_tag="RD477_001121"
     CDS             42681..43982
                     /locus_tag="RD477_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDT7633497.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(44051..44650)
                     /locus_tag="RD477_001122"
     CDS             complement(44051..44650)
                     /locus_tag="RD477_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7633498.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44904..45785
                     /locus_tag="RD477_001123"
     CDS             44904..45785
                     /locus_tag="RD477_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633499.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45798..47408
                     /locus_tag="RD477_001124"
     CDS             45798..47408
                     /locus_tag="RD477_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDT7633500.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFRLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47485..48135)
                     /locus_tag="RD477_001125"
     CDS             complement(47485..48135)
                     /locus_tag="RD477_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7633501.1"
                     /translation="MCFICSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPNGEP
                     QLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTEICI
                     LRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLAKAT
                     GKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLEDL"
     gene            complement(48435..49100)
                     /locus_tag="RD477_001126"
     CDS             complement(48435..49100)
                     /locus_tag="RD477_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDT7633502.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        14842 a        10082 c         8793 g        15496 t
ORIGIN      
        1 tttaatttat tctatttctt tatatttatt ttgtattccg ttacaaactc gcgtacttgg
       61 ttaggttcca gagtgacatc tccaaaactt tcaagtcgct ccgctcctgg cagttcttga
      121 gtctcaaatg tgataccgcc atgatttact aatttattcc cgcgcatttc cggtccatct
      181 tctccaaaat ttgctgtaaa aatcacgatt cctgaagcat ctgtttttac ttggatcgat
      241 actttttcat ctgggcttgt caattccgca gcatttagta aaccagattg ttttttcaac
      301 aaaaatggat gatcgatacc gcccactatc attgattggc gaaaatcact ggcaaagacc
      361 tcacctaatt ttttaggacg ttggaagtca aatgctgtgc cacttactga aactacttct
      421 cctgtaggcg ttgtatcttt attgagcgta gcaaataatc ggctattcaa ccaaagtata
      481 tgttcgtcga ttgactgtgc tggatcaccg gttaaattga aatagacatg atttgtagga
      541 ttgaatactg ttttgcggtc actgatccct tggaccgtca ctcgccatgt atcatcgttc
      601 gtcagcgtat accgtacttc tacatctaaa gttccaggaa atccgttttc catgtctggg
      661 ctagtctgat agaaaatgac cgatgcttcg ttttcaccat tgatgactgt ataatgccac
      721 tgcttggctt caaagctagg tgcaccacca tgtaaacaat gaccagtgtc cgggtctgtt
      781 tttacttcga acgtttcatc atctaatttg aatgtgccat tcgtaatccg tccagccata
      841 cgtccgattg aagcaccgat ataagggtct ttttctagat attcttttgc ttcatcaaaa
      901 cctaagatca actctcgttt ttcattatcg attattgttt gaaaatgaac aacccgtgcc
      961 ccaagatcac tgacagacaa agatgtccca ttttgattcg tgatcgtaat caagtgatag
     1021 ttttcgccaa aattttgttc attgatttcc atgttccttg caccctcttt tctttcattt
     1081 gcactgattc cagtatacat ggaaaccgtt ttcttaacag cttattccgt gagtaacgag
     1141 aaaaaattta ctatattcta ataaaagacc gtgaaaaaaa ggttgtgaca aaaatattgt
     1201 cacaacctct ttttccgaac aaacggtgtt caaaagcttg catctgcggt aataagccca
     1261 accaaacgaa aaataggagg agctattttt gcttggctgt tcttattact tgatgcagga
     1321 cagctttttc acaatctctt tttcaatacg attccagtat ataaccatgt cgtcagtaca
     1381 tagtagatga aatacaatac gaagaagatc gtaaacggaa cccaaatatc tttgtatgga
     1441 ttttgcatga atagaccaaa catttgtaat ccgaataaga catggactac acctaagatt
     1501 cctggtgcta agaataacac gcctacttct ctacggatcg attgtttaag caacccgcgt
     1561 cgtgttccta tcttttcaag catttcataa cgagcaatgt cactcttgct tcctgacaag
     1621 atcttgaaca tcaaacagct agcaagcatg gtcaagaaag ctaaacctag gaagaatccc
     1681 ataaattcaa aaccagagaa tagaccatta tacacttcat acatttcata tttctggttg
     1741 ctgtactcta ctttttgcaa agatggattg ttcattttat tttgagcaac aagtgtttcg
     1801 attggtttca ggtttgcttg gaagtctttt acctgaacta gctgtaaagt ggattgtggc
     1861 aagttcagtt gatcaaattc tgattgactg actaccttga tttctttttc ttgctgttcc
     1921 ggcaattcat agcttcgaag ttcctcttgt acagtcagtt cattcatttt ttcagcagga
     1981 actttttctt tcgtgatctt cggcggtgtt ccatcttcat gttttatcat gatcaatggt
     2041 tgagccgcaa attcacttgc attatagtaa atcgttcccg catcttcttt ttgagtataa
     2101 gaagcagaaa gtgttggtga aagttctttg attttttgct gatcttctgc ccgaggatta
     2161 ttcatcacta gatcatatcc agaattgttt tctgtcaagt ctgcaatttc atttttaaaa
     2221 cctaatccta ccgtcaaagc gcccagtgcc aaggcaaaca gcatagcgac cattgagagc
     2281 atttgtgtat attcacgaat acgaaagttt aactgtgaca atgtgaaatt gttcaatttt
     2341 ttcaatgaaa tctgttctga tttctttagt aatgataaaa agaaaatgat cacagaatgg
     2401 aaaatgaaat aagttcctaa tacgatcgta actaaggcta ctcctatacc aaagacttga
     2461 taggtcccca cgtgtttcat catatagtaa ccgcagccta gaaaagcaat tcccagtact
     2521 acttcaagga aaaacaagaa tttatgctgc ttgattcttg ttggtgtctg atccgcacga
     2581 atcaatgcta atatcggttt tttgacaatc gaaaaagcat tgacaacagc agctaataaa
     2641 aacaaaacag caaaaaacaa aacagtgatc aacaacccgt gaatactaaa aggtgtaaaa
     2701 tgcgaaatat tgagatctaa ctgatccact aataatcggt ggacgatact tgtcaatcct
     2761 acaccgacag ctgaacccac taaagtagct gcgatcccta ctaagaatgt ttcagaaaaa
     2821 atcatctgtg cgatttttct gccttttgct cctagcatca taaacattgc atagtctttt
     2881 tgccgcatcg tcatcaaaaa ggaatttgca tataaaatat aaacgaatgt gatgatccct
     2941 agtaaaacag aaccgaatcg gaaaatgata actgtagccg caacgatcgt attacttttc
     3001 aaaaaagctt cgttgcttgc cattgattca aacatgtaaa aaatagcaga tgccataact
     3061 aatcctgaga ataacaccag gtagtctctt aaacggctct tgatcccagt aagtgataat
     3121 ttccaaagca tagtcaatct ccccctactg ttccaagttt ccaagaatag atagtatttc
     3181 tcgatagaag gaatcacggc tttgatctcc acgtttcact tcttgatgga taccgccatc
     3241 cttgatgaat aaaatacgtt ggcaataact tgcagaaaac ggatcatgcg tcacaagaag
     3301 aatcgataca tgatcttttg tattcaattc gtccatcgta tctaaaagat cccgggcact
     3361 tttggaatcc agcgcacctg taggctcatc ccctaataga atcgttggtt gtgtgatcaa
     3421 tgcacgtgcg gcggcgaccc gttgtttttg tccaccagaa atctcagaag gatagctttc
     3481 caaaatatga gagatggata aacgttctgc aattttttgg acacgttgct tgatttcttt
     3541 tggtctgacg ccttgtaaag aaagaggaac agcgatattt tcttgtgctg tcaagctttc
     3601 taataaattg aagtcttgga aaataaagcc aatctcattc gcacgaaaat ctgctagttg
     3661 atttcctttt aatgtggtca catctttttg attgattcgg atatgcccat ctgttggttt
     3721 atctaatgtg gataaaatat tcaaaagtgt cgatttacca gaaccagaag cgcccatgat
     3781 cccaataaat tcgccttttt ctacatcgaa gctgatgccg cttaatgctt gcgtcttttt
     3841 ctcattgttt tttccgtata cttttgttac attctgtact tcgactattt tgttcatttc
     3901 tttccctacc ctttaattag tttttccagt gctgttttcg gaacgtcttt tttttcgact
     3961 tcttcatacg aagtgacacc tttatttttg ttccaggtga tttttaaata tgctccttta
     4021 cgaagcggtc gagtcaattg tcccttgaag tccagttctt tttccttacc ctctttcgta
     4081 tagccaggca ggatatatct atagttgact gtttcttgtc cagagtctga ttttgaaact
     4141 gtttttgtac catctgtcgt aatttgtaca tagtattctt ctccacccat aactacgtaa
     4201 tctgcgattt tcacccctgc aagtaccaca acgatgaaaa caagtagacc gatcagaagt
     4261 tttttcactt tttccccttc tctcttactt tatatactga ttatagaaaa aagcgaacaa
     4321 ttctgccttc gaatcaacta tcattcttct ttcaattttg tcattttctt taaaacaact
     4381 aaaaaaagag ttcaagacaa taatccgtcc taaactcctt cttcctatct tacaaaaagt
     4441 atgtcactta cggtctttca tgtcgcctgt attgataaca ggttgcgttc ctttgtctgt
     4501 aatgattgag acaagtaaat tattgatcaa ttggactttc cgatcatcgg tgaaattgat
     4561 ttcttgtcca tcttcgattt gctctaaggc catttgagtg attgtgacag ctccttcaac
     4621 aatggtttgt cgagcagaca aaatggcttt tgcttgttga cgttggagca tggcactagc
     4681 gatttctgtt gcataggcta aatggttcaa gcgtgtttcg attacttcga cacctgctac
     4741 tgctaaacgc tcttgcagct ctttttgcaa ctcgtcagac actgccgtcg tgtttcctcg
     4801 aagcgtcaaa tcattctcat tgaatgtatc atacgggtat tggctagcga tatgccgaat
     4861 ggctgtctca ctttggattt ctacaaaatc ttgataatac gctacatcga ataatgcctt
     4921 tgctgtatca acgactttaa agactactac tgctgagatt tcgatggggt ttccatccaa
     4981 atcattgact tttaaaacag aactattgaa attccgaact ttcaacgaaa cagtcatttt
     5041 ttgtgccaaa ggtatcgtaa ggaaaaagcc attttctcga atcgtaccta aatattgtcc
     5101 aaaaaacaaa attacttttg cttgattagg actgactaca gttgccgaac ttaacaacaa
     5161 taacgtgatc aaccaaagaa aaatacttaa aaaaagtgcc caaactttcg cttgccacat
     5221 gcctaaatag aagaaaaaca atccaactaa agctaatacg accaatccga caatccctgc
     5281 ataaccattg atataaaatg ctcttttttc tctcatgcta cttcctgcgc tcctttatcc
     5341 ttcaataaat atctaatgtt acttttttaa ttttttttcg aatgcgcgcc attacacctt
     5401 ctagttctaa ctctttgtct tcagtgatgt accaaccgct tgcctgtgtc cctccggctg
     5461 cctgcggtaa ataatccaat tttgttcgaa cccagtgctt ttcttctgta aaatagtctt
     5521 ctaccaagac ctctagatcc atccctgctg aaagaggcaa atcttcccga acagatttaa
     5581 agataaatcg ttccttcttc ccatcatact tcaatattcc tgtatcgata atcggtaaag
     5641 aataataatc cagctctttc ttcaccgtca ataaatgttt gaaaacttca tggtacactt
     5701 gctctaattg gcgaccttct gaattgttcg ggtaattaac catcactttt tctttgtctg
     5761 gatcatagaa ggtttggctt agttgagtga tataagtatg taatctttcc atttctgtct
     5821 ttaacttttc tctttgacct tcttctacct tcatatactc tttcaccctg ttttcttttt
     5881 agatattttc tttcttattt tactatagtt atgtgcgtta agcgatgaaa aacagcaaag
     5941 attggaagga tctttgctgt tttttcttaa aatatccagt tgagtacagg atcaatctgt
     6001 ttcattgtcc tcttgatccg tcgtaatatt gatcgatgtg tctttagcag tgatctttag
     6061 ctttttctgt ccttgttcta acaaagtatt tgttattggc tggtagtctt tttcaatata
     6121 agattcacca gacgtttgga tagtatagcc actgttttcc atctcatgca aagataaatt
     6181 accaccgttt aggatgactt ttccttcttt gctgatcaaa ctgtcttcta gatggaagtt
     6241 gctgttttca cctgtgacga tcaagttatc cacattggcc tcatctagtc gaagtgagcc
     6301 taattcatct ttcgcagtta ttgttcctcc tgtaaattca ttgaacgata cccagccatc
     6361 atgtgtctta gtattcactt ttttgattga tacttcgttg atatcaattg cactattatt
     6421 cccttcgatt gtcaattcag ataattcatg atctgcaggg acaacaatag tgatttgtgc
     6481 acttgtgtct tctaaactaa tgattgcacc tgttgatttt ttgactgcat tcacagtcaa
     6541 cacgccattt ttctccttta tttctggctt ttctgtctta tcataagtcg tttttatctc
     6601 aaaatccaat ccgcgtatga ccgaaactgt ttgattttct gcatggatga cgattttgtt
     6661 tgcatccttg attttttttg tctcagtaac catatcgatc aaccgaggac cattatccca
     6721 agtcagactc ttttgagcgc ctaatgtata agttgcacct gctaggaggc caccagtaag
     6781 catcagacca ataccggtaa tcaaaaaatg tttcaatctc aatttatgtg tcctccttca
     6841 aacgcccgct gaatgcagat ctctttttgt aataaaacgt cgtcccatcc atttaacaaa
     6901 gttcatcatg acataaaaaa gcccttttgt tcctttcagc aacagtggcg aaataaatac
     6961 tgcgcttcct gtggcgaaca gaccgacacc gataaagaat aatccactca gaatcgattg
     7021 tccaaacaca ctgattcctg agatgatcat gaataggcca acacctaaga tactgataca
     7081 taaaacatag atactaaaaa tgagcgctag taaagcaacc aatagtcccc caataaccaa
     7141 tataaacccc agtgctagtg gaatgaggac aggagaagca aataaaccga taatgattaa
     7201 ccaaatcagg cgaatttgtc tttttggatt tttcactaac tcgttttctg cttgttcttc
     7261 ttctgaaaag taactgattt ttaattttaa ggcaaactgc tttggtgtac catattcatc
     7321 aatgatctga tctaatgttt ttccagaatc taaaaaatat tcctcataat actggatcaa
     7381 atcaaaacga tcgtcttcag gaatttcttg aagatgatcc acaacttcat tcaaatattc
     7441 attcatttgt gctctcctcc aataaccggt ctaaagaatc tcgaaaaaca atccattcat
     7501 caataatttc ttctaaatga cgacttccca ttggtgtcaa cgagtaatat cgacggtttc
     7561 tgccttcaaa aggttcatca taggtctcaa gatatccttc ttttttcaac cttctcaaaa
     7621 ccggatatag tgtagattca ctgacagaga gactttcttg tacgattttt gttaaggcat
     7681 acccatacat atcttccttg cttaatgctg ctaacacagt accatcaaga agaacagttg
     7741 gaatctggat gtgcattacg tcaccttcta ttctcaagtg taatatttta tttcaatcta
     7801 tattatacaa catataatat agtttcaaaa tcagtttgtc aaatacaaaa aaactgacta
     7861 aaagatttga tcctttttag tcagcaaaaa atggcttctc tttgattttt attataacag
     7921 tggcataatc caatttgtcc acatccagct cagtggcatg ataacgatca ttgcaggtat
     7981 catgtcagct actgggaaca ttttcacgtt gatcattcta aaaccggtag cgaccatcaa
     8041 gaatccgccg caagctttaa aatctaagat catgtctggt gtagtcaatg gataaataaa
     8101 ttttgccaat aagaacaaaa tataaaaaat aatgaattgg ggaattgcta tcacagaaac
     8161 gacataccct aaattacacg caaaaatagc tgctgtgaaa aaatcaagga cagatttaga
     8221 aatcaaaatc gtactgtctc ctgtcatccc agaatctaaa cttccataga tccccgtacc
     8281 acttgcacaa aatagaacaa tgaccgtcac taatgtgctg ataaattcat cgtgggtcat
     8341 accaagcttt tcactaggga agattttaga aataggtact tgcatcagcg cagccccctt
     8401 atttatccca ttacctaaat gaacaatcaa accaatcgct gttcctatca ctaaagcaaa
     8461 aataacagcc ggcatatttt tcatcggcgc aatggaatag attcccatcc ccatcgaaca
     8521 cacaccaaaa accatcgtca gctgtgcttt aaaatgttca cttaatttat ttcctactaa
     8581 tccgccaatt agtccgccaa acaaaacgga acctacattg atcaaaactc cagttggcat
     8641 caaaaaactc ctctcttttt ctacgaagcg aaaaaaatca gaaaaatttc tgtaattatt
     8701 gctttcgctg caagaaagat tgtaacagac agagaagatt acaccaattc attttttgtg
     8761 ttaaagtatt ctttattgga ataagtagga ggaatatgat ggatatccat aaattcgaag
     8821 tttttcttga tttagccgaa acaatgaatt tcaccaaaac tgctgatcga caattcacca
     8881 cccaaggaaa tatttctaaa cagattcttt cgttagaaac agaattagac gtcaaattat
     8941 tcgaacgcgc ccatagaaaa atcgaattga ccgaagctgg aagcttattg ctcccatatg
     9001 taaaaaatgt cgtagaacaa taccatgcta tgcaagaagt gcttgaaacc tatacgaaag
     9061 acaacaatct ttcattaaat atcttaacga taccgacaat gatcaattat aaaggttttt
     9121 ccaaaatcac cgagtttttg aaacgccatc ctgaatttac agtccagttg aaagaagtgg
     9181 aaagtgtcga attaacttac aatgacgcca atgaacatag tgacacgatc cactttgcta
     9241 gaagtttcca ggccccacca agttcgattg agtggttgcc tactgaaaca gacgattttg
     9301 tagcagttct acctaaaaat catcctttag ctagtaaaaa gaaattggat ctaagcgaac
     9361 tgaaaaagga gaactttttg ctccttggcc ccactacgaa cttgtatcaa cctgtactag
     9421 atctgtgtcg cgaagctgga tttgaaccaa aaatctctta taaaggagca agaatcgact
     9481 tgatcatcaa tatgattgca aatgacatgg ggattgctat tgttatggaa aaaacagtca
     9541 agaatctatt aaaagaaaat actgtgattc tccctatttc tccaacaaca gagagttatc
     9601 ttgcatttac tcgtaaagtc ggtgagcatt ctttggcttc agatacattt tgggagtatt
     9661 taaatcaaga agaaaactaa gccctacatt ttctgtccat ctatccaccc aaaaaaagag
     9721 agcgacgcat gactttcgct ctctttcttg catcatgtcc ttgtttcaat gacacctcta
     9781 acatttctcg tagttcaata agaaagcagc gatttttatg ccaaaagaac cgtaagaaca
     9841 tttccctaat cacaaaaaaa cgactaaaaa cccctaaaaa ccttcaatag aatttttagg
     9901 ggttttattc tttttcctat tgtttactta accgtatcat tgctgtcgtt cctactcctt
     9961 cgatactgct ataatcaata cgtccatcat gcagttctag aattttttta accagtgaaa
    10021 gtcctagccc attcccagaa atttgacgac ttgtatctcc ttgataaaat ttttcaaaca
    10081 ttcgatctct ggtctcttca ttcattccta cacctgaatc ggtcacttcc agtacaatct
    10141 ctgtggctgt ctcaaatagt ttgatatgaa tctgtccatt gatttgattg tactttatgg
    10201 cgttatccat gatattcagc cacacttggt acaaaaactc ttcattgcct gtgtaattta
    10261 ctgcagcaag ttcgatatcc aattctagct gttctttctc ccatttaggc tgaaaaaaaa
    10321 cgattacttc tctgatttgt tcatctaatc gatactcttt cttttccagt tggatacgct
    10381 ggttttccag tttgttcaat tttaagatgt tctctgttaa ttgtgaaagc tgcgtgatac
    10441 tttcgataat ccgatataaa aatatttggc gttcttcgtc agagagattt ggggcttgta
    10501 acaattgaac atatccttgg attgttgcta gtggtgtttt gaattcatgg gatacactgg
    10561 acacaaagtc attcctcagt gtctcgatac tattgagttc ttggaccata acgttgaaat
    10621 ctttatacag ttgctgtact tctgctactt tctgctgttc atccattcga atcgaaaaat
    10681 ctcccgttgc taccagactc atatttgtcc ctagcgcact gattggatgc aggatacgcc
    10741 gcccaacaaa aatcgatatt cctgtaccca cgaccatact gaaccccgca aagacgaata
    10801 acgggtataa cgttggtttt ccagccgatt cagcaggcga gatagaatgt cgagacaata
    10861 agaaaataaa accgagaaaa cacagcagtg tcactaagat cgaaatgaag acaatactag
    10921 caaaatagat ccataattgt gaaaatagct tttttttcat ctttaaataa ccgccttata
    10981 gcctagtccc cgtactgtga tgatttgaaa atcttccgtc ttttcaaaac gggtccttaa
    11041 acgtttgata tgtacatcga tcgttcgttc ttctgtattc gtatctaatc cccaaatatc
    11101 atccatcaac tgttggcgag tgaagatctt gttgggataa gacagtaatt tatacagaag
    11161 cagaaattct ttttgaggca gaatcaacga atgctgatct tgtaacactg tataagcatc
    11221 tagctctaat tttgtttgtc cgattatcaa gctactgctg taattgatct ttgaacgtcg
    11281 aagcaatgct tccacacgta ggatcatctc attgacatcg atgggttttg tcatataatc
    11341 atctgctcct aatttgaacc cgcgtttctt atcctcaaaa tcggcctttg ccgtaatcat
    11401 tagaacaggt gtgtcaaatc tggtgctacg taatgagtct agcaagtcat agccatccat
    11461 tttcggcatc atgatatctg taatgattaa ttctatacgt gtcgcttcta gttttttcag
    11521 cgcatcctct ccattcaatg cggtgatgac tgaaaagccc gctcgttcca acacagaacg
    11581 gtaaagcaac tgtaattgcg gatcatcttc tactactaaa atacgattca ttgatctccc
    11641 tctgtttttt gaaaaatttc tagatttttt cttgtagttc atcttcagtt aacatttatt
    11701 tcgtatacta cgatcattgt tctgatatac ttattagtat accgaatatt tcaatacggg
    11761 agaagggtga ttatgcttca attaaaagat ataaaaaaat attataaagt cggcgaaacc
    11821 accaccaaag cattggatgg tgtgtctgtc gctttcagac aaaaagaatt cgtagcgatt
    11881 ttaggtccta gtggttctgg taaaacgact atgctgaatg tgattggtgg actagataat
    11941 tatgattccg gtgatatggt aatcaacgga aagtctacta aagactttaa ggacagtgac
    12001 tgggatgcct accgaaacaa ctctatcggt ttcatttttc aaagttacaa cttgattggt
    12061 catctaggca ttattgaaaa cgttgaactt gggatgacat taagtggtgt ttctaaagac
    12121 gaaaaacgaa aacgggcaga agatgcattg caccgagtcg gcttgacaaa tcatatgcat
    12181 aaaaagccaa atcagttatc tggtggacaa atgcagcgag tggccatcgc tcgagcacta
    12241 gccaatgacc cagatatcct tttgtgcgat gaaccaaccg gagcgttaga tacagaaaca
    12301 agtatccaga tcatggaatt gatccaagag ttatcgaaag aaaaattagt gatcatggtc
    12361 acacataatc ctgaactggc ccatcaatac gcagatcgaa tcattgaatt ttctgatggt
    12421 aagatcttaa cagattccca tcctcacatc gaacgaccaa aagatgatca atttaactta
    12481 cgcagaacaa agatgagttt ttggacagcg ttaaagcttt ccttcaacaa tatccgaaca
    12541 aaaaaaggac gtactttctt gacctccttt gcttccagta ttgggatcat cggaatcgcg
    12601 atcgttctgt ccctttcttc tggattccaa aaacaaatcg ataacacgca agctgaaacg
    12661 atggcgaaat ttccgattac tatttctaag gtaacgacta accagactcg tgacgatgcc
    12721 ggattgggtg caagcaaagc ggattatcca gacagcaaaa cgattactgc aaaagtcagc
    12781 gaagaagata aagcacagca cacgaataag attgatcaaa catacgtcga ttacgtaaca
    12841 gacatcgatc ctaacttaag taataatatc ggatttaccc gtactacagg aatcaatctt
    12901 ttacgtgatg taaatggcaa agtacagcct gtcagcttct cgaatcaaaa tcctgatacg
    12961 gaatctcttt ctttatctag tacgatgtca gcgatgacag gtgtgggagt gtcaagtttc
    13021 cctacacagc ttgacactag caaagaaaac tttttaaaag acaattattc tttacttgcc
    13081 ggaagttatc cagcctcagc aacagatgtc gtgctgatcg tagacggcaa taataatact
    13141 aatatcaacg cattgaagaa tcttggattt gatgtgaaag aagacgaaaa acttgatttt
    13201 gatgatatcg ttggtacaac attcaaatta gtaaacaaca atacttacta tacaaaactt
    13261 ccaactggga attttatccc taatacagat tatgatgcga tgtatcagaa tgcttctgat
    13321 gaattgaaga tctcggggat cctacgcgtg aaatcttctt ctacaatgaa tctcttatct
    13381 ccaggaatcg cttatagcga tcaattaacg actcaaatcg tcaacgaaaa caaagaatca
    13441 gaaatcgtca aagctcaaag agacagtggt gtaaatgtct taactactga aaaagttgac
    13501 gaaaatgcca aacaaacatt gcttagttat ttaggtggag acagcttgcc ttcaagtatc
    13561 atgatttatc cgaacaattt tgaagacaaa gaaaagatct tggattatct agatgattac
    13621 aataaaggca aatcagatga agataaaatc atttatactg atttagctgg aacgatgact
    13681 gaactgactg gcggattgat ggatgctatc acttatgtcc ttattgcttt tgcagggatc
    13741 tctttagtaa caagtatgat catgatcagt atcattactt atacatctgt gattgaacgg
    13801 acaaaagaaa tcggtgtact aaaagcttta ggtgcacgta aaaaagacat cactcgcgta
    13861 tttgacgcag aaacgtgtat tttaggtatt tcttctggga tattaggtgt gtttattgca
    13921 tggctagcta cgttccctat caatagcatc ttatacaaca tgacggatct aaaaaatgtt
    13981 gctcaactaa atcctgtcca tgcgattatt ctagtgatcg tctctactat attgacgatg
    14041 cttggcggtc atcttcctgc acgaatggca gcgaaaaaag acgccgctat cgctttgcgt
    14101 gcagaataag gttgtgacaa ccacatttag atttgagtca taagagcgct gaaatagtag
    14161 aaaacagcaa gaaacgagga agtaatatgt tccttgtttc gcgctgtttt ttttaattat
    14221 tttttataat tctggtatac ttttctttcc tttgtaccac aactcttctg gcgtatacct
    14281 gtcttttcca tttatgaata ctgttccatc tgcttcaatc gttacttcag tcccaatcca
    14341 catattacga gaaagtgcga gaagattttt tctttttcct cttccttttt ttccaaattg
    14401 caaggcatcg gttatctctt ctgtccaatg agatttcacg tattttactt ctccgttttc
    14461 aaatgtaact ttcaaaatat tcgctatatt ttccactttt actgctttag gaaatgactc
    14521 ctccattttt ttcactttcc ttcctccaac actaagtatg cgctttcttt tagtgataag
    14581 tatatcatag tttattctat tatcaaaaaa acgcatccat tccattgtag aatactttac
    14641 cgaacaagtt gaattgtttt cctctttttc accttttact atagataggt aaagacaact
    14701 aaggaggaat ctctcatgac agatcaaccg tatgatttaa gaaaagtcat cgaagaacta
    14761 aaaacacttc caggtcaata tcacgaaaca gatattgaaa tcgatcctga agcagaatta
    14821 tctggtgttt accgttatat cggtgctggc ggtacagtaa aacgtccgac tcaagaagga
    14881 ccagctatga ctttcaacaa tatcaaaggt ttccccggca cacgtgtgaa catcggaaca
    14941 atggctagtc gtaaacgtgt aggtgaaatt ttgcaccatg attatcgaac acttggtcgt
    15001 ttcttgaaag acgctgtaga aaatccaatc aaacctgtga aagtagacaa agcagacgct
    15061 ccagcacaag aagtcgttca tctagctacc gatgcagact tcgatattcg caacattttg
    15121 cctgcaccta caaatactcc tgaagatgct ggtccataca tcacaatggg tgtagtctta
    15181 ggatcagatc cagaaaaaac aatgagcgat gtaacgatcc atcgtatggt tctagaagat
    15241 aaagatacga tcggtatgta catcatgcct ggcggacgcc acattggtca cttccaaaaa
    15301 caattcgaag ccttgaataa accaatgcct atcactatca atattggttt agatccagca
    15361 attgccattg gcacgacttt cgaaccacct accactccac ttggctacaa tgaactatgg
    15421 tgtgcaggag ctttgcgtaa cgaacctatt caaatcgttg atggtatagc agtcgatgaa
    15481 gtgggtattg ctcgtgctga attcatcatt gaagcagaaa tcatgccaaa tgaaacaatg
    15541 caagaagata tcaacacgaa ctcaggaaaa gcaatgccag aatttcctgg ctatactggg
    15601 gatgctaatc cagctgtaca agtagtgaaa gtcaaagcag ttactcaccg aaaagacaat
    15661 ccaatcatgc aaacaacgat tggtcccagt gaagaacacg tatctatggc cggtattcct
    15721 acagaagcaa gtattcttgc cttggtcgat aaagctattc caggaaaagt agtaaatgtc
    15781 tacaatcctc ctgcaggtgg tggtaaattg atgaccatca tgcaaatccg taaagaaaac
    15841 gaagcggatg aaggtattca gcgtcaagca gctattcttg ctttatcagc cttcaaagaa
    15901 ttgaagacag tcattttagt cgatgaagat gttgatattt tcgacatgaa cgatgtagtt
    15961 tggactttca acactcgttt ccaaggagat aaagatatca tcgtgatccc aggaatgagg
    16021 aaccatccat tggacccatc agaattacca gaatataatc ctggctctat tcgtttccgt
    16081 gggataagtg caaaaactat ctttgacggt acagttcctt tcgatatgaa agaacatttt
    16141 gtacgtgcaa aattccaaga agtagaagat tgggaaaaat atttgaaata atgaatgatc
    16201 tgcaactgtt ttgacatcat aaaaaggagt gcggaaaatg gaacaattgg cacaacggaa
    16261 gaaaaaaatc gtgattggtg taagtggcgc ttcaggcacc atttatgcca tcaacctagt
    16321 gaagaaacta aaagaatatc cgatgataga aacacacgga atcatcagtc catgggcaaa
    16381 acagaactta aagctggaat ccgacttatc actaactgaa ttcgaaagcc tgtttgatta
    16441 ccattattca aacaaagatt taggtgctgc tgtcgccagc ggatcatttt tgaccgatgg
    16501 aatgatcatc gtaccggcaa gtatgaaaac agtcgcaggt atctctgttg gtttaggcga
    16561 caacctgatt gcacgtgctg ctgatgtaac aatgaaggag caacgaaagt tagtcattgt
    16621 tcctcgtgaa acgccattaa atgccattca cttggaaaac ttgactaaat tagcacgact
    16681 aggtgtgcaa atcataccac caattcctgc tttttataat cagcctcaaa cgatccaaga
    16741 tcttgttaat caccatacaa tgaaactttt ggatcaattt gaaattgtaa atgagcattc
    16801 aaaacgttgg cagggtgatt aatatggaaa aagaaacgat agttgaagta accaaactag
    16861 gtttcacgat ccaagctgct gttaaagtaa tcggtaaaga cttattgatc actttaacag
    16921 gcggagatac accacatatc ggaacagtca ctacattttc tcgtgataca gagatgcaaa
    16981 ctgtacgttt ccctagccac gatggtcggt tccataaaga cgatgtctta gccgaaaaaa
    17041 tagctgtgat catcaaaact gccgttccgg gaagttgtac gatcacttcc ggtatccatg
    17101 ttgaccatat ctccaatgca caaatcaacg cctcttttcc gatgtcagaa gagttaggtt
    17161 acaagatttt agattggttg gaaaaaatag atttccatat caaagaacca gtttattata
    17221 aagatggtga aaaaccatta tgaaagaaga ggttgtgaaa tcagcgttat gacctgagta
    17281 tgagagaaaa aaatggaaaa atagcttctc atattttgcc gtttttgagc taataccgag
    17341 ggacagctga ttgaacgccg tttatcaaga ggttgtgata aagccgcttc gtttcgagta
    17401 gtaagaaaaa ttttgtaaac atgggtttcc acatttttgc ataaatttga cttactaccg
    17461 agaaactggc ttttgaacac cgtttatcaa gaggttgtga cacgtctttt gtcacaacct
    17521 cttttatttt tgcttcactg taactgctac aatatcaccg atttcttttc cgatgttttt
    17581 acgaatatct tttcgtagtc caaggatgta gcagactgaa ccatctggat ttttcacgcc
    17641 catattcacg atactgcctt catacggcac attgtcaaaa gttgcttgga ctttaactct
    17701 tccagcaccg aattcttttc ttatatcata cggaaaaata atatatgccc cgcccttgcc
    17761 gacttcggaa gcatagattt ttgattcaaa agaataaatt tccctcattt tatttctcct
    17821 attttttaca tctagtatag catagcaaaa aaaaagaagg aactgtgaca aaagtcttgt
    17881 cgcagttcct tttttcgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    17941 atagtcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttaaac
    18001 ggcaggctca caacctcttc cttaaacggt gttcaaaagc ttgcatctac ggcactaagc
    18061 ccaatcagat gaaaaatatg gagtgctatt tttatctgat tgttcttact gcttgatgca
    18121 gagcagcttt ttcacaacct cctacatggt gttcaaaata ttattcgcct tttgcttctt
    18181 tcactaattt tgcgaatgct tgttgttgga attcagcagc taaggctttc gtacgttgtt
    18241 cttgctctgc tttgctcatt tttggatttt cagtcattgc ttttaccata ccgtcttgaa
    18301 cataagcttt tgcttcactt tccatcgtac tttgctgttt cttcactaat tcagctaact
    18361 tggctgcttg gtccgtactt aattctaccc atgtctctgg gattttgaat gtattcactc
    18421 ggctgtcata ttcgtgattg ttatcagcaa tattgaacca gaatttatac atgtcgtttt
    18481 tgtattccca ataagtagtt ttcgtcactt tttcagcatc gccgtctgtc ttttctactt
    18541 tatttgtttc gttatccgta ccagttgctt ttggtttttc ttgtttttca tttgttcggt
    18601 aaagatacac tttttctttt ccattcccta aagctttata caaaagcata tctgctcctt
    18661 gggaatcagc actggaaaca attttttctg tttttgtttc cgttactttt tccattccaa
    18721 agtgattgga caaattagcg acgatcaaac ttagtgaagc gacgaagatt agtccgaaaa
    18781 tcaaagataa aaaggtttgc catgtttttt tagcgaacac gttcgttaaa gcaaaagcaa
    18841 gcaagctgac aatcaaaact ccaataatca tgcattatcc cccgctttca ttagaacagg
    18901 ttcttttttc ttcaataaaa aggcaataat aaagccaaga atcccaaaga tgatcgctac
    18961 gataaacgcg gcatggtaac ctgacagtgt ggcattgatt gcctgatctt tataagctaa
    19021 tggcgtatta tccaatacag atttgcttgg gatatttgct tttgtcacat tcgtcaacac
    19081 actgatcaaa atagctgtac caatcgagct tgcgatttga cggaatgtat tatttaccgc
    19141 tgtcccatga ctgatcaagt tatttggcaa tgcattcata ccagaagtcg ttaccggcat
    19201 catgaccatg gagatcccaa acatacggat cgcataaagg atcacgatat ataaaactgg
    19261 agtcgttttt gtcaagaaag caaatggtgc agttgccgct gtcaaaatga acatccctgt
    19321 gatggctaaa cgtttcgcgc cgtatttatc gaaaatcgca cccgtcacag gcatcatagc
    19381 ccccatgatc aatgcacccg gcaacaacat caagccagaa tggaatgctg attctccacg
    19441 aatattttga atatacaatg gtacgaccat ttctgcccct accatcgcca tatttgttac
    19501 accagctaaa acagctgcaa tcgtgaatgt agatgatttg aatacacgaa gttccaagaa
    19561 tggatgttcc aaatgtaatt gacgccatga gaacaaggcg atgaaaatca caccaacaac
    19621 taagaaacca attactttca agcttcccca gccgtcatct cctacactag aaaatccgta
    19681 aagtaaacta ccgaatccaa tcgttgataa aatagcggat aaaatatcaa tgctaggatt
    19741 tgacaatgga ataacacttt tcattaggaa gaatgctaaa accaagacaa gaacaacgat
    19801 tggcagtacc ataccaaaca agtcacgcca atggtagtta tcaatgatat aaccagataa
    19861 tgtagggcct aaagcaggag caagtccaat aacgatccct gacatcccca ttgctgatcc
    19921 tcttttttct ggcgggaaaa tagatagcat gatattttgc aataatggca tcgatactcc
    19981 cacaccgcaa gcttggatca aacgaccgat tagtaatgta ctaaaattag gtgctgtaaa
    20041 gcaagtaatc gttccgatca aaaagatcgt catggcagaa agatataaat ttcttgaact
    20101 gaatttattg atcagccatg ctgttattgg gatcatgatc ccgttgacta gcaagaaacc
    20161 agtcgttaac cattgcacat cagacgctga aatatcgaaa gctttcatca atgtaggaaa
    20221 agctgtcgtc aataaggtct gattcaaaac ggtacaaaat gtaccaatta ataagacaac
    20281 aactaaaagt gtccgattat atggttttcc gtaaatatct acgggttggt tgttactcat
    20341 atttctcctt ctttctgaat tttttctgaa actactagaa tactttagtc ctaaattcgc
    20401 tatttgtcaa cctacttgac acataagtta atacggatat aattaaggga agcaagagga
    20461 caaaaggagt gacaaaaatg gttggggctt cccttaaacg tttgttggaa aatgatcaat
    20521 tggttgttgg catcagtgaa ttaagtgata tcgtaggtgt gtctccacgt caattaaggt
    20581 attgggaaca aaagggattc attcgttcga tcgaagctga tgcgaattgt cctagaaaat
    20641 atcgtttacc tacagtcata aaagtagaac tgatcaaaaa atatttagat gacggattta
    20701 ctttgtcaaa agctgcagaa aaggcagaaa tccgccaaaa gaaaatgcac catgtccgta
    20761 aagttttttc aaaagcaatc aaagatatcg aattgatcga cgaacgctat acgattttac
    20821 aaatcggtaa cttcaaagaa gagaatgcta agatgtacat cattcatgac aacgagacag
    20881 acgaattaac ttacaagctc cttcctgtcg atgaaaaagc tgactacgaa cagttgttaa
    20941 aagaactcta aacgccacta atctcctaag taccaagctt tttaataagt agatgctaca
    21001 taaatacttg aaaaaaggcc tgtttgaaca taagaatatc cgaacggtga tctgcgtttc
    21061 tttcatatag aaaggaattc tccatcactg ttcggatatt tttttatttt tttcggttac
    21121 tttcttctaa tacatctgtt agccaatttg catattctgt ttcccgattg atcgcatgcg
    21181 ctaaaatcag gtagtgtccg taattttcat gtttttcttt ttcagtagga aagatcgttt
    21241 tttttcgctc taatagatga agtcgttttt cttcatgaag gtcacgctgc tgctgaataa
    21301 tttctactaa tgccggatca ttcacgtctt tgacgaaata caatttcaga acaaattcgt
    21361 ctcgattaac cggtagttca ttggtgggtg tatgaatcca ctctgccaat tgttccttcc
    21421 cttcttctgt taattcataa actttctttt ccaatttttc acctgtgatc tctacttgat
    21481 gctgaatgat tccttgctct tccatttttg caagttcagg ataaatctga ctatgttttg
    21541 cttgccaaaa ttctcctatt tcatttttga acgattgatt caattcatat cctgtcatct
    21601 tcttcttact taataaccct aacaaaatat atcgaagtgt attttttctt gccatcagtt
    21661 ttccctcctt gacaaatctt taaatccatt ctatcataaa tgagtaatcg aaaacatgta
    21721 gtttattaca tgttttaatt tataaggaga tgcttaaaca tgaaagaatt taaagaattg
    21781 gaagatttta ttggaactca ctttatctat acgtatgaca atggttggga atatgaatgg
    21841 tatgccaaaa atgaccatac tgttgattac cgtatccatg gcggaatggt caaaggccgt
    21901 tgggtaaaag accaagaagc tgatatcgtt aaattgacgg acggcgtatt caaaatcact
    21961 tggacagagc caacagggac agacgtggca ttggacttta tgccaaatga aaagaaactt
    22021 cacggaatga tctttttccc taaatgggtt gaagaacatc cagaaatcac tgttactttc
    22081 caaaacgaac atatcgcaga aatggaagca gctcgtgaaa aatatgaaac ttatccaaaa
    22141 ttacttgttc ctgaatttgc tacgatcact tatatgggtg atgcaggtat agacaatgaa
    22201 gatgttatta gcgaagaacc atacgatgga atgccagatg atatccgtag cggaaaatac
    22261 ttcgatgaaa attataaacg agtaaaataa ggttgtgaaa gagaggttgt gaaatcagcg
    22321 ttctgacctg agtattagag aaagaaacgg aaaaatagtt cctcatgttt tgccgttttt
    22381 tgagctaata ccgagggtca gctgattgaa caccgtttat caagaggttg tgaaatcagc
    22441 gttatgcttt gagtaataag aacgctagtt agaaaatagc ttttcatatt ttctaggcta
    22501 gtgggcttat taccgagaag cagccgtttg aacaccgttt atcaagaggt tgtgaaatca
    22561 gcgttttgac ctgagtatta gagaaacaaa tggaaaaata gttcctcata ttttgccgtt
    22621 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaaagat tgtaacaact
    22681 gcatattcac acagttaaat tagtgacttc aaaatgaaat aatgctccca gcaaaaaatt
    22741 aggctgggag cattatttcg ttattctgtt catcaaaaat cagcgttttg attccatttc
    22801 atcacaagtt cctcctcatt tactgcttca tctttttgct gtgtttgaat tgtaaaacct
    22861 tgtgaaagat agaaatgaat agctgattga tttttttgat agactgtcaa agtcagtgtt
    22921 ttattggttt cttttatttt tttcattagt tgcgtaccga ttctttgttg ccggtattcc
    22981 cttttcacaa agattcctgc gataaactga tcaatcaccc cagcaaaact aataatttct
    23041 tgatctattt ctgctaagta aatcactgca tctggtaaag ctttcctaac agattctact
    23101 tgattctccc aataagttga tggaatgaat tcatgagaat ccaagttagc atgcagccaa
    23161 atcgatagaa cagattccag ttctttggca gacaattttg ttgttgtgcg gatcattttt
    23221 tctctcctta cttattcatt gattttaaaa taaaaaccat ttttttgaag ctcttttaca
    23281 agttttcgcc cctcaaaaaa gtatatcttc tcttgtttta cttttctaat accaaaagaa
    23341 acagcaactt taggatcatc agctgaaacg attcgataag ttccaccagg caattgatcc
    23401 agcttgactt tcccatgatg gtcgctttgt actgaaaacg gcacaccatt tctagtaagt
    23461 gtttttttct tatgtttcaa aatgaatgaa ctaccattta ccggtttccc tgacgaatca
    23521 ctaataataa atgaaaaatc aagcactttt ttacttaatc tatactgata gattagtcgt
    23581 ccgataaaca ctaacagtat gactacagct attatcgttc ctgcaagaat aaaccattgc
    23641 cgcatggttc tttcctgatt tccttttcct tcttcttttt ttaccgattc gttataagga
    23701 atccgatgtc ctgtcactag caaacgatgc gtgttgatca tatacggcgt acatgtaagc
    23761 aaggtaacta agtcttctcc ttctgaaatc gttagaacag acgtgtcatc tggcagtacc
    23821 acttgtatat ttttaacttg ataagccaat ttctccccca aaacagtcaa aataaacgtg
    23881 tctccgtttt ctaaacgatc taattcggta aataaccgtc gattaggtaa accgctgtga
    23941 gcagagatca ctgaatgcgt cgaatttcca ccaagcggga aagaggttcc ttgcaagacg
    24001 cctgctccat tttcaaggat cctaccattg agccgatcat aaagtgaaat atttaattgg
    24061 atcttaggta tattgatact tccgatcagc cattcctcta attggctgga tgttgtactt
    24121 tcatttctgt tcttttcatt gaaaggatca gtaccaggat tgaatccttg gtcagttaat
    24181 cgttggtttt tctcctccat tttctttcgc tgcacttcaa tttcagctgc ggtccgtttt
    24241 tgtacttgtt cgatacgttt ttgatcaata aaactattta aagaatccac ataaaatgga
    24301 tataaagcga tcagtaaccc tacgacgata catattaata aaaacaaacg aaacaaaagt
    24361 cgacgagact gtttttgctt actctgcttc acatgtgtcc tcccttgcaa gtttttttgt
    24421 tctttctttt attatatggg ttcaggaaaa aaatagaaaa tgaaagccga tgaggttgtg
    24481 aaaaagctgt cctgcatcaa gtaataagta cagcccaaaa aaatagctac tcatattttt
    24541 tgttgggttg ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt
    24601 gtgaaaactg cgttatgctt tgagtaataa gagcgctagt cagaaaatag cttctcatat
    24661 tttctaggct agtgagctta ttaccgagaa gcagctgatt gaacaccgtt taccgagagg
    24721 tcatgaaaaa gctactttgt ttcgagtatt aagaaaaatt ctgtttcaaa acaaggagat
    24781 gcaacaaaac ttatgtcgca tcctctctcc ttagaacttt cacttatgtt tttcttgtaa
    24841 aattcataaa tattttcatg aattttctaa tttcttgcct acctctcgca taggatatat
    24901 gatgaattta tctaaagttt gcaaaggagt tcgacaattg aaaaaatatg gcccattttt
    24961 agcaattttt ttcattgctt tggcatttct catgcctcat ttattcacac ataaactaat
    25021 tttgggtgct gatgccccat tccattacaa tcggttctat gatgccgcgc aacaaattaa
    25081 atatggggat ttccattatt ttccttccct ttatggattc cagcaaagtg gcaggatcgt
    25141 gaatgccgtt tacggcccat ttttcgctta tttacaagga ttgatcgtgt tgcttgcagg
    25201 tagctggttt cattatcaga ttctttcaaa tttcattctt tatcttattt caggattttc
    25261 tatgctgatt ttactgagat atgtcaatgt tcgaaattgg gtaagtgtac cagtagccgt
    25321 tttattcatg agtacgtatt ctattcaata ctggtctatc aaccaaggat tcactagctg
    25381 ggggacttgt ttttatcctt tatgtatgat ccctattgct gatttcgtcc ttcataaaaa
    25441 gttccctgtt atcaaagtcg ctgtctcagt gggacttatg acacagatcc atttactgtc
    25501 ttcgatcatg ttagctttga tgtatatccc tttttatctt tattactttt ttaaccagac
    25561 tgaaaaagga aaagcactgc ttcaactgat taagtcggtc ttgctttatt ttgttctgac
    25621 agcaaatatt tgggcaggat tagctattgt ttacagtggg aataagattc ttcctccctt
    25681 tgttaacaga gaaatgagtc aaaaggcgat agatctggat ggcagttatt ggctccatta
    25741 ccctaaggcc tttcctatat tgatattatc aggttttttg ctttttattt tctttcaaca
    25801 aaagaacacc ttattccaaa aaatcgtgtt gtacacaagt cttgttttct tttttctttc
    25861 tactagcttg attccatgga cgacattgat cgaaaaaaat gtgcctttta ttagtttgat
    25921 ccagtttcca tttcgatttt tcgtgccatt tacgctattg cttctacttt accttgccct
    25981 ttcactgaac cagtggtctg aacaaaagtg gttccgtctt ttaagtatat ttggcattct
    26041 tattttttca gcacagacga tccagaactt ataccagcat ctggaaaagt gggaaaatga
    26101 aacatttgtc agtcgacata cttatctatt cgatacgccg gaagaagcaa ggaaaacatt
    26161 cttcagcaaa gatatgtcta ccagcttgtt cgttttccaa aaaaccacac ctgactatct
    26221 gccaatctat aaggagacaa aagacaataa atacgaccgg tataacgaag aaatactaga
    26281 aaacgaaaca acttttataa aaacacatcc aggcggtctt ttgacaatca agtggaaaaa
    26341 cactggggac aaaactgttc atatccctgt tatcgtctac gatcggactg tgctacagca
    26401 aaatggaaaa acattgactg attatgaagt aacagatatc gggaccccaa tcgtgaagca
    26461 gcaaaaagga atcaacgagc tcacgcttca ttatcaaacg ccaatctatt tctatttcat
    26521 tctttctctt actttgattg gctggtttac cttgctatgt ttatttattt atcatagata
    26581 caagctttta cgtgcttagg caaaagggat aaatacatcc gatcaaaaaa ctctccaaac
    26641 ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat gttatatttt
    26701 ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga ttggactatc
    26761 gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga cgaaaataaa
    26821 atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt ccacagtagc
    26881 tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc aactttctct
    26941 tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga aaaaaagtgg
    27001 tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa agccactttt
    27061 tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat catctatctt
    27121 tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa attgggagac
    27181 ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt cttcatattt
    27241 atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt gttcctttaa
    27301 aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat tctttaccgg
    27361 acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt ggttgatttc
    27421 ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt cttgcaattt
    27481 tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt tcattggttt
    27541 tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca caacctctcc
    27601 agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca ggagctattt
    27661 tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct ctccactaaa
    27721 cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat aggaggagct
    27781 atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctcctta
    27841 cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct tgatcactac
    27901 ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt cttcgttttg
    27961 gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt ggccttcttt
    28021 gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca tatggatcaa
    28081 aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga aaatacttgt
    28141 cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag caaagccgtc
    28201 acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa tcacttctcc
    28261 agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac ctgcttgtcc
    28321 atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca gcagatcttc
    28381 gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc cattgtcttt
    28441 gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta ctttttctcg
    28501 atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga tattttcttt
    28561 tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg ttccgctgtt
    28621 agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg gtgtagcaaa
    28681 gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa aagcgatcac
    28741 acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta acggtgtccg
    28801 agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat ctgccatcgc
    28861 aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag caaacataaa
    28921 catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg ctgaaaaata
    28981 cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg caaaagccat
    29041 tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag ccggcgtcca
    29101 gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat tcactaaatc
    29161 agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg cctgtgcgcc
    29221 tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg tcagcatatg
    29281 atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg gtgctagaat
    29341 cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc cagcctgtac
    29401 gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga taacaacaaa
    29461 cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc tataattata
    29521 gtatttatta taagccattg ccccgacaaa tccagcgatg atccctacga atactcccat
    29581 attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga tcttcgtacc
    29641 gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga caccaaagat
    29701 agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg ctccgcctgc
    29761 tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa gattaccaat
    29821 aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg tccaatcagg
    29881 attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg gcataactgc
    29941 taccacaacc attaaagcct ttccaaattt ttgccaaaat tcaaaactaa atatcttttt
    30001 cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc gtgcgttttc
    30061 ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa aacctataat
    30121 acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct tttttctatg
    30181 aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg aacgtataaa
    30241 acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta ttgatccatg
    30301 gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat ctttaacaag
    30361 tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact caggggacca
    30421 ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg tcggctggtg
    30481 gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact ttatcgcgat
    30541 agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag aagactttta
    30601 ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca ctacttctac
    30661 taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag aatcagctta
    30721 tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg tatcaaaatt
    30781 ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga atgagattga
    30841 tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa acaactttgg
    30901 gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa aatcagtcat
    30961 ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag aattatctca
    31021 aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag atattccaga
    31081 agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca attatatcca
    31141 agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg atgtggtcat
    31201 cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac tattctctac
    31261 ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag gagaaaaata
    31321 cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc ttgctttagc
    31381 caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc cgcaagcaat
    31441 ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca ccttcactgg
    31501 tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa atggcgcgat
    31561 tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt tgaaaaatga
    31621 aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc atttttcaaa
    31681 acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg agaacaatat
    31741 caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca cgcgagaaaa
    31801 ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac tagccaaatg
    31861 gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa tctttgtcca
    31921 gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca caagcgatct
    31981 tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca tcaagcaggc
    32041 agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag aaaagcaaaa
    32101 aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc caagtatcca
    32161 ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt accagcgaac
    32221 agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc acatcacttc
    32281 catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa ctgcccatga
    32341 tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt tccatatcaa
    32401 ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt tcgagttatt
    32461 agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag cggatcgtct
    32521 agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt gatttagacg
    32581 gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt ggtcaggaaa
    32641 tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc cgagaagact
    32701 cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag gaagaatttg
    32761 cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg atcgaaccaa
    32821 aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa aaaatcaaga
    32881 ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg ggtcttacac
    32941 cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct gcgccagata
    33001 tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc gggatcgaag
    33061 acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt ggcgtgggac
    33121 gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt cttacgatcg
    33181 attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt cgtaccagtt
    33241 ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt cgttcttctc
    33301 aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa atcagaaaat
    33361 gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt ttttggagca
    33421 actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca tagattggag
    33481 aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt ccaattttgg
    33541 gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt aaaagatgaa
    33601 ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac aaaaaacgaa
    33661 ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc gaccaaccat
    33721 gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca gttaacggtc
    33781 tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa ttggacaaaa
    33841 aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat ccttgcatgt
    33901 tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc caaaacttcc
    33961 ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc taataaaagt
    34021 atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg aaaacagatg
    34081 cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt tcatttcccc
    34141 aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac gatttatcgg
    34201 ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt aaaagatgtg
    34261 gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga tcatcctagt
    34321 atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg ttatgttcct
    34381 aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt aatcttaccc
    34441 cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat ggaagcaatc
    34501 aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa aaattttgaa
    34561 ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg ttttattcta
    34621 gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca catccctttc
    34681 actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga taatgataac
    34741 cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa gaatatcctg
    34801 tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg ttatcaaaaa
    34861 gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca tccggaggat
    34921 tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt ggtcattgac
    34981 gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt cattccagag
    35041 acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca cccttacctt
    35101 acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa gctgcgcgaa
    35161 ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg cttgatcaaa
    35221 agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact aaatacgctg
    35281 accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta acacattgtt
    35341 tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
    35401 cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
    35461 taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt ctgtcccagc
    35521 ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa ccgcctaata
    35581 ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc aacgcgctaa
    35641 ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga gaattgacgg
    35701 tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca acgtcatcaa
    35761 aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct ccaacgatca
    35821 gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt cggtccatct
    35881 tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt aacccaccta
    35941 atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc ccgcctcgag
    36001 aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc atcaagatgt
    36061 ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg agagcagctg
    36121 tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc ccaaataaaa
    36181 taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg ttttggatca
    36241 tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc gtataaaaaa
    36301 tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata tgttcgaata
    36361 gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc gagcagttat
    36421 tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg aatccaagta
    36481 aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat gaactgccga
    36541 cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa cgcacagaaa
    36601 caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc catgaagcaa
    36661 ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg attcctttga
    36721 tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa aacaacaaac
    36781 acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg atcagtactc
    36841 ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa atatttttat
    36901 tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaact attcacttgg
    36961 caggacctcc tactaaatca gcgacaaaac gtcggctgaa agacttattt ttttattttt
    37021 cttctaaact tttttctacc tcaatccccc aagaatcttt cttgaagtga ttgatgatca
    37081 atggaataac gaacatgacc actgtgatag ctgctactaa taatgcatca ctcaatgtcg
    37141 ctgtacctaa tgtagcaaat acgacaacga tgatagagaa gatcagcatg cagctgacta
    37201 cccaagctaa tccgtttccg tttttaccaa ttcggtaagg acgttcgaat tctggatgtt
    37261 tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta cacgatacaa taaataacag
    37321 ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc atccattaca ccataaagca
    37381 aagcaaataa tgaaataacg attgattgtg ttagaacgac atttctagat acgccgtatt
    37441 tgttttcttt atggaatccg aattttggtg gaaggaatcc ttcacgagct acttggatga
    37501 ttgtttttga tggtccagta acccaagctg ataattgtaa aagcacacca ataaatacca
    37561 tgaagctgaa gatatttccg atcacttctg gtaagcctag gatttgacag tcaatcaaga
    37621 taggctgcgt gatatttgct aattccattt ttccatctgg tacgacattt gctacaagca
    37681 tcgcattgat gacattcaat aaaactaatc cgatcaaagc gataaatacg ccttttgtat
    37741 aattttttgt tgcgtctttc aaacgaggca tgtaaacaga actcatttcg atcccaacaa
    37801 aaataaatgt gataccagct aagtatttca atgaatcaat atgttccaag tcagggaaag
    37861 cttttgacca tgaaaagtca cctagatagc cgtttgagac tagtccgact ttgaaggctg
    37921 ccaaaacacc taaaacgaac atgatgacta ctgggatata tacccctagc catacaccga
    37981 tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa gatcgtgatg atccagtaga
    38041 taactaaaat acaacctaaa acgaaccagt gattattgcc aagttctaca ttaccaaatg
    38101 tgtttcctaa caatggtcct aaagtagaag cgaccattac catacctggg aacatttgca
    38161 cccataatag ccatgcaacg acaaatcccc atttgctgcc cagtgctgtt tttacccata
    38221 actgaggacc gccttcttga ggaagcatcg tggaaagctc accggaaacc attgatatcg
    38281 gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat caatgtccaa ccgactgcgg
    38341 ctaacgtagg aatactccga accgttgcac acagtgccat tgtcagaccg ataaaggtcc
    38401 caagacccat tttacttact tcagatttac tcaccgttga cactctcctt gattttttgg
    38461 ataaaataac ggtgtctttg aagtagtttc agcaataaac gaaacaattc agcgaatatt
    38521 tgaggggatt ctttgagttt attgcttgaa gggtttcttc aaagacaacc tcaaaacaaa
    38581 gggatcgaag agcttaggtc tccccttgct cttcagatcc aaacatgcgt cagaaacagt
    38641 tagtattcaa gctgttttct aacatgatta ccttgcttat tttgcttcgc ttgccaataa
    38701 ttggtctgcg tagatttttt ccaatttttc ttgaagagct gctttgattt tttcagcata
    38761 ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt acgctggcac gcaatacagt
    38821 tacttttccg gcacggttcc attcttcatc tgagaatcct agttggttca caaattgtaa
    38881 tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt gaagttaaga actcattacc
    38941 gtaaatgctt cctttcacgt atgaagaata atcatatacg tcatggttca atttattcat
    39001 tgcaactaag tcatcgttgc ctttttcttt gaatacatag tctaccatat tgaaatctgg
    39061 ataagttaat ggatgaactt cgatttcttt gtctcctact ttaaatgaca agtcattcaa
    39121 gaagttgtag aaacgatgtg caccttcgat tgatgcaccc attaatttac catatcctgt
    39181 gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg gctgctgttg cccctgcttt
    39241 tgaaccttcc aagatataag ccccaagtaa tgctgggata tctgcgcctt tttcaaatac
    39301 atatgtcgca aagtaagaga taacgtctct catgcggata tctttgatga cgataccacc
    39361 ggcagaatat ggaacatagc ccattttatg tgggtcaatc gttacagatt ctgcttcttc
    39421 gattgcttta tatgcactgt gtacttcttc taagatataa tctttgtttt ctgtaaagac
    39481 attgtatttg taatgaacat cttttaaatc ttcaaatgga atgaagttgt tgtcttcgtc
    39541 taagaaaatc gcacggccgt atccgccata agcagcatct acgtgtaagt agaagtaaat
    39601 accgtctttt tctaatacgc gacgcaatgc aacgatttta tcgataccgt cgattgctcc
    39661 ttcttctgtt gatcctacaa caccgactac acctaggatt ggtgtttttt cagctgctag
    39721 accacgaacg attttttcaa gttcgttgat gtccatgcga tagttgtggt caactggcac
    39781 tggaatcact tggtctaaac cgataccgat gatgtcggca gcttttagcc atgaatagtg
    39841 tttagtttgt ggaaccaacc attttcctaa tttttctaaa tgttttccgc tacgtgctga
    39901 gtgagctttg atttcgtcga ttttttctgg gacgctgtct aacagattca tgatttcttc
    39961 tgttgacaag ttcattaatt cccaatcgct ttttccagca actagttcag gtgtcacttc
    40021 tttcattgca agcggcaatg atttgatgtt gcgggcatac caaagtcctt ctaagtttgc
    40081 taaagaacca tcagcaacga tgtggcccca gccatcttta tagctcatta attttgcaaa
    40141 ttccattcct acttcttctt ccatttggct tgttgcagga gaagattcat aggcaacgtt
    40201 attaccattc catagcatag caaaattgta cgctagtaaa gatggcatca atgtttcaga
    40261 gttcatatga ccccagtaac gtccggcatt atgccaagga acggaatggg tacgcatccg
    40321 cgctgaaatt tctgatagaa catcctttgt tttattcaca gtgtgttcaa aactagcact
    40381 gcttttttct tctggcgtaa taataggcat gtcttgaggc atgtagtttt gacgccagcc
    40441 taaatgttca tccaccaatt cattcaacaa agctttatag atttggccgt tctcagcttt
    40501 gtccccaatg aatagggcat ttaggtttag atctttcgac aatgattcac tcatagtgaa
    40561 accctcctaa aaaaatattt aaattttgtc gagccctaca ttacacctgt tcttctattc
    40621 tgtaaactaa gttttggcct gatttttaat catattttaa tatgtatcaa ttatccgaat
    40681 tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt tacaaatgta aactccctcg
    40741 caaagattgt attaacgaca accagcgttc tattttagga tgggtttcaa aattggaatg
    40801 ttgtaatagt atttcccttt ttcaaggtaa aaacttacta aattctaatc aattactttg
    40861 gccaaaaagt aatttttctt accttttcga ataaccacaa acttaccatc gaattcatcg
    40921 gatggattta tgacaaaatt taaatcggta acgcggtcac cgttaatact tatagctccg
    40981 tttgaaacat cttcacgtgc ttgacgttta gatggttcaa tttttgtgga aacaagcagt
    41041 tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg gcattttacc gaatccttgc
    41101 gcgatttctt ccgcatttag ttctttaata tttccagaga acaatgcttg cgttattttt
    41161 tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg taacttcttc tgccaaacgg
    41221 cgttgtgctt cacgtttctc aggttctgtc tctacttttt tcgctaagtc ttcgatttct
    41281 tcttgagaca agaaagtaaa gaatttcaag tatttcacta catcgcgatc atcttgattc
    41341 aaccagaatt ggtagaactc aaatggtgaa gttttcttcg gatcaagcca aacggcaccg
    41401 cctgcagttt ttccgaattt cgttccgtca gccttcagca tcaatgggat cgttaaaccg
    41461 aacgcttctg cttctggtcc ttctaatttt cggattaggt ctaatccagc tgtgatattt
    41521 ccccattggt ctgctccacc gatttgcaat tgcacatcgt atgttttatg cagatgcaaa
    41581 aagtcgatag attgcaggat ttggtaagta aactctgtaa atgagattcc tacttccaaa
    41641 cggctggcaa cgatatcttt tgctaacatc gtgttgatgt tgaagttttt accgtagtct
    41701 ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt agttgttcac aaatgtgata
    41761 tttgcatctt taccaaacaa ttttctcatt tgattggaaa gtgcgtccac gttatgttgc
    41821 acttgttcca tcgtttgcaa cacacgttct gttttgcggc cgcttgggtc gccgatcgtt
    41881 cctgttccgc cgccaatcag aatatatgga tgatggcctg ccaattggaa tcttttcatc
    41941 atcataaatg gaatcaaatg tccgatatgc atcgaatcac cagttggatc aacgccgcaa
    42001 taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc tttcttcgtt tgtttgctga
    42061 ttaatggcgt cgcgccaagt taattcatca ataatattca ttttactgac ctccgtgtag
    42121 tttttttatg aaagcaggaa cctgctagca tgtgtaatta attatttctt caggatagga
    42181 taggaaataa aaaaagtccc ttggcatata agccaaggga cgaaatctcg cggtaccacc
    42241 caatttgtat gtctagatat ccaacatacc tctcgaacat ttaacgctgt cgcgtggcaa
    42301 tcactgccaa agactcctga aatgtaattc gctttaaagc atgtactggt ttgcagcgac
    42361 caccagcttt ctgaaacatt ggctttaaca actacttcgt ttcgatcttc gtcttgttat
    42421 tattaatatg tgtctatcat agagcgcaac tttttctttg tcaacagaaa aaggatattt
    42481 tttttaaatt cctcattttt tcttttttga atccgctcta attccgaaca ttttattttc
    42541 aattattgtt aaaagaaata actttttctg tattttttct gcaatttatc gagaagtcac
    42601 tttactttgc ctcttattct ttcttcgtta caataaggaa tgggacaagt aaaaaaatga
    42661 aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat tattttagga gccacttctg
    42721 ctttaactat cgttatttta attctaagtt tgttttttgt catccgatca tctacaaaaa
    42781 aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc aaaagaaccg acagatgata
    42841 agcaacaact agttactcaa gcaaaaaatc tggctcaaag ctacgattat gaccaagcga
    42901 ttgcgttatt ggaaaaagat agtaagaacg gatctactga aacacaacag cttcttcaaa
    42961 cactaaaaaa ggagaaagaa caactgatcg actggtctga tcctactcag atctctcatg
    43021 tctttttcca cagcttgatc gtggacccgc aaaaagcctt ccactctcaa caggcacaag
    43081 gatacaaaga ttatatggtg actgttgaag aattcaaccg ttcgatcgaa caactttatc
    43141 aaaatgattt tgttttagtt aatttgaatg atttgatcca aaaagacgaa caaggaaatc
    43201 tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc attggttctt tcacaagatg
    43261 atgtcagcta ttatgaatat atggataatt ccggttttcc tagccgattg gtcgtatcaa
    43321 ataacaatga aatcaaaaat gtctataaag agaaagataa agaagaaatc ggtgactttg
    43381 acatggttcc tctgatcgat gcctttgtca agaaacatcc tgatttttct tatcgaggcg
    43441 caaaaggtac cttggccctg acaggttaca acggagtttt aggttatcga acatcaaaat
    43501 ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa agaagctaaa aaagtggcgg
    43561 aacagttgaa gaaagacggc tggagctttg cttcccatac atggggtcac ttgaatatga
    43621 ctcaagcttc actagaagat gtaaaaaaag acaatgaatt gtggcagcaa gaagttgcac
    43681 ctatcctagg aaaaacaaat attctgattt atccatttgg tgcagacatc agcgactggc
    43741 agccttattc agcagaaaat caaaaatttg cttacttgaa gagtcaaggt ttcgatattt
    43801 tctgtaatgt cgatgcttct acaccagctt ggggacaatt aggtacggat ttctatcgaa
    43861 atgctcggat caatatcgat ggcattcgtt ttcaatctga tttagacgga aaaaatccta
    43921 ttcttgatcc attcatcaat gtccatgatg tctatgacaa agaagcacgc gaatctgctt
    43981 gattttacgt catttatgaa ccaaaaaagc cgaactgaca ttttatcgtc agtttcggct
    44041 tttttatgct ttatttcttc ttcggtgcga ttcccttgat caggacttct gtccattctg
    44101 ctgtatatgg aagcagtttt gcgtatatct ccggcttaga aattccttct accattgctt
    44161 gaaaatagag gatcaagaac tcatccgaat atttcaaatc cacttttcct tctttccgcc
    44221 ctcgatggaa aagatcaagc atgatactga aactttcttg cgtatacgca tccatcgcgt
    44281 cagaaaattc ttttttttct ttttttgtat agtatgtcat caaatcgata taaaattgtt
    44341 tgttcactcg attgagataa cggattttgt tttcactcat ggcaatcagt gtttcttcaa
    44401 atggaagatt ttccgcaatc aacgctttcg cttcttgccc taaatcattc ataaatgcaa
    44461 aaaatacttc ataaatcagc ccttccttgc tgccaaaata tttaaaaatc gtcgctttac
    44521 ttacctgagc gttctcagct accttatcca ttgtcactcc agcggttcct atgtctgtgt
    44581 tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt ctcttgtgct ctcttttcaa
    44641 aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt ataatatata tagttcaaat
    44701 ttattctgag gtttattata actatctatt gagaaagtat caagctagca attccttgtt
    44761 tatgaattca ttgacagaaa tttataaaaa gaatattatg tactaaaata ataaaaaaag
    44821 ttttataaaa aggtataaaa cctttttatt aaactattta atcttgttta gtatatattt
    44881 ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa agtcacacat ttgaaaaaga
    44941 gctttggtcg tttcgaagct ttaaaagatg tctctttcac cgtacattca ggtgaagtcg
    45001 ttggatttat cggaccaaat ggttctggaa aatccacgac gattcggaca cttttaggga
    45061 ttataaaaaa agacggcgga acggcaacga tttttgggca agatgtatgg gaagatagtt
    45121 tatctatcca tgaacgtctt tcttatgttc caggcgatat ctctttgtgg ggaaatttga
    45181 ctggaggagc gatcatcgat ctcttcatga agcttcatgg aaaagggagt gctcaaaaaa
    45241 aagatgaact gattcaacgt ttctctcttg atcctaagaa aaaagcaaaa aactattcaa
    45301 aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc tgttgattcg gatctctatt
    45361 tatttgatga gcctacttct ggtttagatc ctttgatgga aacgattttc caagaagaag
    45421 ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc ttctcatatt ctcagtgaag
    45481 tcgagcgact agcggataaa gtcgtgatca tacgcgaagg aacactcgat gaagaaggaa
    45541 cactcgatga attgcgtcat ttgactcgtt cgaccatcac tatagaaaca gaagcagaga
    45601 atagtcactt agcacaattg aacggtgtcc atgactttgt gcaaaaaggt cacctcgctc
    45661 aatttgctgc tgataacgaa gctttcgatg tgattttagc tgaagcgtcc aaattaggtg
    45721 tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt attcatgaga cattatgaag
    45781 gatgaggagg aagcgaaatg actgataaat tttcacgctg gtccaccctt ttttctcaaa
    45841 accttagccg tgactggaaa aagatcctga tatggctagt cggtgtcact gctttttctg
    45901 gtggttttgt ccctgcgttt gaagaattga cttccggaca aggattagct ggattatacg
    45961 aaaccttgca aaacccggca atgattgcaa tggtcggacc aactccagct tcgaatgctg
    46021 ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt gcttttttgc agtttgtttg
    46081 ctgctgtgat ctctgtttta cacgtggtga gccaaacgag aaaaaaagaa gaacttggtc
    46141 tgattgaact tattcgttcc tttcgagtcg gacgtcaagc aaactcttta gctgtcatcg
    46201 ctgaaagtat tctgatcaat ttgcttttag caatatcgat cagctgtacg atgatttttt
    46261 tccaagcgga ttcgatcact gcagaaggtg cttggctttt taggctatct gtaggtagta
    46321 tggggatttt ggggagtgcc gttgcactgt ttgctgcaca actgatgcca agttcagcca
    46381 gttcgaatgg tctgtcttta gcgtttttag gtggacttta ccttctacga gcaataacag
    46441 atgtagctga ttcctccctt tcgatgtgga atcctttagg ctggtcttat ttgacctatc
    46501 catttacaac taacaactgg attcctttgc tttatttgtt tttgttcagt ctaatcttga
    46561 taatcagttc atttgtttta gaggaacatc gagatttgaa caccggctat atccccgaac
    46621 gtgaaggaaa accgtatgct aaacgttctc ttttatcctt gcgtggttta ttattctatc
    46681 taaataaagg gatgacaatc agttggctta tcacttttgg tttgatgggg gcggcatacg
    46741 gctctatcta cgggaatatg caacagtttt tagaaagtaa cgacttaatg aaacagatgt
    46801 ttatccaatc tggcgtctca attgaaaaaa actttacaag cacgatcatg gtcgtcttga
    46861 tttgtctagc tgctatttta ccaattgcta gcatcaatcg tttatttaca gaagaaaccc
    46921 acggacgtat gaatcagctg ttcgcaacaa aaatttctag gaaccaactt tattggacca
    46981 atatgttact agctattcta tttgctatat tggcaatcct gattagttct ggtgcactag
    47041 ggctttctgc tttggcttcg atgaaggatg cgaaagacat ggatctattg atgtttctta
    47101 aagccgggtt caatttcctg cctgccgttt tagtgtttat cggaattagt ggctttctat
    47161 tcggatggct tcctcgaatc ggtaaacttg cttatatcta cttagcttac tcctttgcac
    47221 tcaattactt tggcggaatt ttggatttac cagaatggtt ttccaaaaca gctgtcccta
    47281 gctgggttcc acggatgcca aacgattctt ttgatctttc cgtgttcctt acgttatcat
    47341 gtatcagcct tgcactgttc gtccttggtt ctattggtta taggcgaaga gacttactag
    47401 aaggataata aaaaagcatg tacaatttca tgctagcctc caaatctggg tcaaaaatcc
    47461 aacatttgga ggtcattttt ttatttacaa gtcttctaac tgctctttat caatgatcca
    47521 gacttctcga ttctttcttt ttacataacc attctcctct aataatttaa attttctaga
    47581 taaagtttcg ggggtcgtac cgataaatgc agcaagttct ttcattttca ttggcaggaa
    47641 aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga tagacagcga ctcgttcttc
    47701 tactttttcc atcatcaaaa aagttgtctg ctgctgtact tttgttaatt tctcaacgtt
    47761 gattgctaaa agtttcagac taagggcggg ataatgcagt aagagttccg agaaatcttt
    47821 ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg ccaaataaag actcatttgg
    47881 aacgcctagc aactggctct ctccctcata tccgcccggc tctattactc gtaaaagctg
    47941 ttcctttccg ctagcagata attgatacac tttcatactg ccggaggcca cgatcactaa
    48001 ttgaggttct ccattcggcg caagaatctg ttctcctttt tccacatagt gatgtttgac
    48061 aagtgcgtgt actttttctt gatcttgctg tactaagtga ttgaacaaag gaacaaagga
    48121 acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt
    48181 ctaataaaga aacataactt cccttattcc aactgataat tttcttttat catagcataa
    48241 acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt
    48301 ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt
    48361 gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc
    48421 ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt
    48481 tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat
    48541 agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt
    48601 gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc
    48661 catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac
    48721 tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa
    48781 tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga
    48841 gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt
    48901 tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga
    48961 atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct
    49021 gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa
    49081 tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa
    49141 ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac
    49201 agccaaacaa aaa
//
LOCUS       DAPBBG010000017        48706 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPBBG010000017 DAPBBG010000000
VERSION     DAPBBG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 48706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.017"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            23..646
                     /locus_tag="RD477_001127"
     CDS             23..646
                     /locus_tag="RD477_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633503.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="RD477_001128"
     CDS             1096..2145
                     /locus_tag="RD477_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7633504.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="RD477_001129"
     CDS             2142..3023
                     /locus_tag="RD477_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633505.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="RD477_001130"
     CDS             3026..3796
                     /locus_tag="RD477_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7633506.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="RD477_001131"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="RD477_001131"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDT7633507.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="RD477_001132"
     CDS             5107..6270
                     /locus_tag="RD477_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7633508.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="RD477_001133"
     CDS             6387..6797
                     /locus_tag="RD477_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7633509.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="RD477_001134"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="RD477_001134"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7633510.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="RD477_001135"
     CDS             9370..9654
                     /locus_tag="RD477_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633511.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="RD477_001136"
     CDS             9814..10050
                     /locus_tag="RD477_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7633512.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="RD477_001137"
     CDS             10047..10445
                     /locus_tag="RD477_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDT7633513.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="RD477_001138"
     CDS             complement(11112..12056)
                     /locus_tag="RD477_001138"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDT7633514.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="RD477_001139"
     CDS             complement(12352..13296)
                     /locus_tag="RD477_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633515.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="RD477_001140"
     CDS             13517..14731
                     /locus_tag="RD477_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7633516.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="RD477_001141"
     CDS             14753..16768
                     /locus_tag="RD477_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HDT7633517.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="RD477_001142"
     CDS             complement(16851..18224)
                     /locus_tag="RD477_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDT7633518.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="RD477_001143"
     CDS             complement(18250..18534)
                     /locus_tag="RD477_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7633519.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="RD477_001144"
     CDS             complement(18536..20551)
                     /locus_tag="RD477_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7633520.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="RD477_001145"
     CDS             complement(20730..21035)
                     /locus_tag="RD477_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDT7633521.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="RD477_001146"
     CDS             complement(21124..22155)
                     /locus_tag="RD477_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7633522.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="RD477_001147"
     CDS             22362..23237
                     /locus_tag="RD477_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7633523.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="RD477_001148"
     CDS             complement(23389..24231)
                     /locus_tag="RD477_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7633524.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="RD477_001149"
     CDS             complement(24323..26200)
                     /locus_tag="RD477_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDT7633525.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="RD477_001150"
     CDS             complement(26197..27618)
                     /locus_tag="RD477_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDT7633526.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="RD477_001151"
     CDS             complement(27621..31010)
                     /locus_tag="RD477_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDT7633527.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDCVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="RD477_001152"
     CDS             31267..32658
                     /locus_tag="RD477_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633528.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="RD477_001153"
     CDS             complement(32731..33378)
                     /locus_tag="RD477_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDT7633529.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="RD477_001154"
     CDS             complement(33386..34072)
                     /locus_tag="RD477_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633530.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="RD477_001155"
     CDS             complement(34078..35151)
                     /locus_tag="RD477_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633531.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="RD477_001156"
     CDS             35373..36047
                     /locus_tag="RD477_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7633532.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQPPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="RD477_001157"
     CDS             36052..37392
                     /locus_tag="RD477_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7633533.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="RD477_001158"
     CDS             complement(37541..38527)
                     /locus_tag="RD477_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDT7633534.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="RD477_001159"
     CDS             39251..40282
                     /locus_tag="RD477_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7633535.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..41923
                     /locus_tag="RD477_001160"
     CDS             40391..41923
                     /locus_tag="RD477_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDT7633536.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIISLSPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42062..42874
                     /locus_tag="RD477_001161"
     CDS             42062..42874
                     /locus_tag="RD477_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDT7633537.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42864..43349
                     /locus_tag="RD477_001162"
     CDS             42864..43349
                     /locus_tag="RD477_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7633538.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43372..43917
                     /locus_tag="RD477_001163"
     CDS             43372..43917
                     /locus_tag="RD477_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDT7633539.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43990..45633)
                     /locus_tag="RD477_001164"
     CDS             complement(43990..45633)
                     /locus_tag="RD477_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDT7633540.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45762..47990)
                     /locus_tag="RD477_001165"
     CDS             complement(45762..47990)
                     /locus_tag="RD477_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7633541.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48023..48502)
                     /locus_tag="RD477_001166"
     CDS             complement(48023..48502)
                     /locus_tag="RD477_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633542.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14819 a         8979 c         9169 g        15739 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgattta tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcacatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaaac cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca ttcgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttgat ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac aaatttcagc gtaccaccag gagtgagtga aggaatataa ttcgagtcaa
    24961 ctgaaaccgt gaatccgttt gtttgttcag tgacagaata atttgttgga tcgatttctt
    25021 tatccccatc atacaagaca tacgcatacg ttcctgaagt ttcatgatca aatgtcaaag
    25081 cggcgtcatg agtatcaatg agtttgaatg tcgtgtactt gttttgtgtt ccttctttat
    25141 cagcgatacc taaaggaata ttgacagaga tctcatattg gattttttct ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt ggacgtatca tttttgattg
    25261 tcttttctac aggagtttga ctatttgcca agatctcaaa cggcgtgttt gtttgattat
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacctcta
    25381 aatgattgac gatcaactga cctttttcaa tcgttgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatccgtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtcttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgatac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt ttttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tgctttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cgtaccggaa gacgcaccgt tttttgcgat ctctgtttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataacgcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct gcctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcggta tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaagacag caccgatgag attcttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacga atttgtagta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac aatccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga atgaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgtcacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagcc tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc accatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagcactt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 agttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg tttctcaatc
    40861 atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa ctggtatccc
    40921 taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc aaaaaacagt
    40981 tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag tgcattccag
    41041 attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc ctttagcaaa
    41101 cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg cactagagac
    41161 ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag aagctttttc
    41221 gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa tgaagacatt
    41281 tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc aaagtgaatg
    41341 cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg atgcaagaga
    41401 tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag ttgggaatgt
    41461 cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg ctttgactta
    41521 ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg atagcggaga
    41581 ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag tggatttgat
    41641 cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt tagatttttt
    41701 ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta tttctttatc
    41761 tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac ttcctcgctt
    41821 gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa tgaaagtgca
    41881 acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta aggaaagaaa
    41941 gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat aaaaatatgc
    42001 caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg aggaaaatgt
    42061 tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag gaatccaaac
    42121 agatttaaaa acttttgaga attttggtct attcggacta agcgccttga cttctatagg
    42181 ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat tgcttaaacg
    42241 acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg ggttattgaa
    42301 ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa atatcccaat
    42361 cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc aattgtatat
    42421 ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta atttggcaga
    42481 agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga tccatctcgc
    42541 taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca caaggatggc
    42601 tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat tttctcataa
    42661 aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg caataacagc
    42721 gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa aatttgttgc
    42781 ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct ggtctggcgg
    42841 tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat gctatgctga
    42901 ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct acgaatggat
    42961 ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt ccaacgggtg
    43021 gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga tatccgcagt
    43081 gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt tctttcagca
    43141 aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt atgatcatcg
    43201 gctatggtat cgccggttgg attttatatg attggccggc tggagtggct tctataccag
    43261 gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact gcaagtcttc
    43321 aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa aatgaagttg
    43381 tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt ggctgaggca
    43441 cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt cgttggagga
    43501 catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac gttaaaagag
    43561 ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt gaaccgagca
    43621 ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc tgttgttcct
    43681 gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa tgatcctgtc
    43741 gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc aatcggtatg
    43801 catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg acaagctcac
    43861 gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa ttattgaaat
    43921 gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat gaactttaac
    43981 gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta gtatgccttt
    44041 agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact gatctgcttc
    44101 ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa gatattgttc
    44161 atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat attctttata
    44221 agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct tatagaattg
    44281 caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg tatttggatt
    44341 agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag tccattgcat
    44401 gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt cttctcttgt
    44461 cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat caacttgatc
    44521 gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa taaatggcgt
    44581 accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc aaaaatcggg
    44641 acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca tatataaagc
    44701 gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt tcaatttaag
    44761 gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa attcgaatat
    44821 catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag tgacaccact
    44881 tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat cggtcaaatg
    44941 ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg cttttactga
    45001 atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta tccctttatc
    45061 caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt cccaatttaa
    45121 gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat gagaatactc
    45181 ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat ctaccccgtc
    45241 gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt cttcaaacca
    45301 ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga tccctaattc
    45361 atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct cttctatatc
    45421 ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga tcggattgat
    45481 ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga tcccattgat
    45541 gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt gatagcctac
    45601 tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta acggtccctc
    45661 ttgattataa ccgattctat tttagaagta tccttgttac cggtaactag aaacgaaaag
    45721 ctcaacgaat ctgctaagtg cgttcgctga gctttatttt atcatagata gcgggcaatc
    45781 agttgaatca tttgcttggg aaattcattc aagtcagtaa tatctacaaa acgactttca
    45841 ccgtagatcg acttgatttc ttctttgtct tggccaatag ctgcggagac aaataggatt
    45901 ccttgacgtt catattcttt caaaacagac tggatatctt cttttgcttt tttgcctgta
    45961 taatccggca acgcttttgg ctgaccgtca ctaatattaa gcaacagttt ggttgttgcg
    46021 gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag caccatcacg attgttttgc
    46081 cttggtttca tagtgactaa tttttcattt aaccattgaa atccgtcttc aaattcttta
    46141 taagaaaaca aagaagtctt ttctcttgac gaacggtctg ctgtatctcc gtaaatcaac
    46201 aatgggatat cgactttctt agaaaattcg gcaatggcaa tcgctgcttt ttttgccgct
    46261 tcgatccgat catcccgtat cattgaccca gattcatcga tccgtacagc taccgctaaa
    46321 gaaggttgct catgtggcgg gttctttttg tcaaaatttc tcaaatcacc atatgctact
    46381 ttgcttgcat tgaaccgctg accttcgtat tttccttttt ggtacgtctc tgtttgttca
    46441 ttatccagta actctaaaat ttgccggcta agtgattcta ctattggcat tacttgcatg
    46501 tgtagctcac gtgcttccgg cgtttttcct tggaaagctg gtcggtgaac gatgagacct
    46561 tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt ctttattcag ttgcttcttg
    46621 atttccttat catgtgcttc catgtcagaa gcagacattt cttgagaatc atctcctgtt
    46681 tgttgttctt tttgattttt tccttcgata tcgctactgc gttctttttg catttgagga
    46741 ggaatctcgt ttttttctat tgtttcttct tgtttctctt ctccctcatt tggttcttca
    46801 gcagacaacg atttcttttc atctaagcgc agaatttctt cagcattttc agtgatgttt
    46861 cgttcatttg cttcttcggc gctaatggtt ctttcttttg tatgctcaga ttgaggatga
    46921 ccccattttt cttttctcaa tatgtcctga agttccagta gcaatccact gtcagacaaa
    46981 gcttgttcaa gaattgccaa ttcatctgaa tcagtggata atttatacaa aacttttgga
    47041 taaatcgact ggagtaaatc ttgtccttgc ttataggtat ttgcccaata aaaataagaa
    47101 cgcattcctg ctacaccttt gatggcgtta gcttttgcag tttcatccaa caaacggata
    47161 atctctgcca tctttaagag aagttcttgt ttttggatca tagttttcgc catcgcacgt
    47221 tctgccatga cttctaaggc aggaagatcc attttttccg catgttgcat ccgatctctt
    47281 aaagattcat ttaatgggcg atttccttgg taatttcgat ttgtagtgat cacaatgacg
    47341 caatctggat ggcgacgaat gattcctgtc ggtaaattca acatcccatt tggttcgagc
    47401 gctgagttaa gtgccacaag aacagaggca tctcggatga cagtcggctc ttgaatctct
    47461 aataaatagc ctttttcaat cgctcgaacg atttcagaag ggtaatagct gtaatggatt
    47521 gcatctggat aatcagcttc gatcctttga actagctctg ctaatttttc tttagcagaa
    47581 aaaggatcaa tgccgtaatg gttctggatc agtgtcagta cagcttcaag tgtatctgtt
    47641 tggtaaatag cttctagtaa ttcttggtct tgttccttat tagaatctac tacaggtaat
    47701 aaggctccga aaacatctga tttgtccata tctgcaaaac aggtcacttt tgtataggga
    47761 agttgcagat cagcagatag tgctttggct aattgtgtct tccccgaccc ggcatctcct
    47821 tccaaaagta cgttactgat tttcatttca ggatcttgcc aattcccttt gatttcggag
    47881 gcgatccgcc attcttctgc acttgtccga tggcttttcg gtttcttcca gatcatttgc
    47941 ttttctagcc gtgaaaaggt tcggttttct gttagtgaga aattctccat gcttctcttc
    48001 cccttttgat catctctgtc tattaatatc ctagctcatc taaaagacgt tttaaaatac
    48061 ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt atcgtaatac aaaatgatgt
    48121 cttcatcgat ctttggattt ttggtatcta cttctaccgt cattcggttc ccttgtaatc
    48181 caatgatgtc tacgactgga ttttctggat cataaaattt ttcataacga taagcatctt
    48241 ggaagtataa acttccttgc gcagctaaga tcgctaaatc taaaatacgt gtgattggta
    48301 attcttccga ctgtcgtgac catttttctc cagtatgtcg ccataccttg cctgagatat
    48361 cgacttttcc acgatcattc cactgagcaa ggcccagtga aagccctttt gcatccatat
    48421 ttaatgcatt tcttccatca actttatcat agtcttctac aacgattact ggtttatgtt
    48481 ttaaatttgt tggtatttcc attattttaa ctcctcatcc ttttattttt agtaaataag
    48541 taaattagta gtttactcat ttactaaact atatgcgaca ttatagagga ttgaagtgag
    48601 gattgcaaag aaaaagagct tcattgatag aaataaaaac accatctcgt tcttttacac
    48661 gctcattagt aacatgtaga aagtccaaga tgatgttgga atattt
//
LOCUS       DAPBBG010000018        48122 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPBBG010000018 DAPBBG010000000
VERSION     DAPBBG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 48122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.018"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(37..192)
                     /locus_tag="RD477_001167"
     CDS             complement(37..192)
                     /locus_tag="RD477_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633543.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            328..1657
                     /locus_tag="RD477_001168"
                     /pseudo
     CDS             328..1657
                     /locus_tag="RD477_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1668..1877
                     /locus_tag="RD477_001169"
     CDS             1668..1877
                     /locus_tag="RD477_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633544.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            2036..2788
                     /locus_tag="RD477_001170"
     CDS             2036..2788
                     /locus_tag="RD477_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7633545.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            2785..3699
                     /gene="pfkB"
                     /locus_tag="RD477_001171"
     CDS             2785..3699
                     /gene="pfkB"
                     /locus_tag="RD477_001171"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDT7633546.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            3717..5648
                     /locus_tag="RD477_001172"
     CDS             3717..5648
                     /locus_tag="RD477_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7633547.1"
                     /translation="MNIKDLVVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(5759..7318)
                     /locus_tag="RD477_001173"
     CDS             complement(5759..7318)
                     /locus_tag="RD477_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDT7633548.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            7473..9707
                     /gene="pcrA"
                     /locus_tag="RD477_001174"
     CDS             7473..9707
                     /gene="pcrA"
                     /locus_tag="RD477_001174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDT7633549.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            9724..11760
                     /gene="ligA"
                     /locus_tag="RD477_001175"
     CDS             9724..11760
                     /gene="ligA"
                     /locus_tag="RD477_001175"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDT7633550.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            11866..12171
                     /gene="gatC"
                     /locus_tag="RD477_001176"
     CDS             11866..12171
                     /gene="gatC"
                     /locus_tag="RD477_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDT7633551.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            12171..13643
                     /gene="gatA"
                     /locus_tag="RD477_001177"
     CDS             12171..13643
                     /gene="gatA"
                     /locus_tag="RD477_001177"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDT7633552.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            13640..15070
                     /gene="gatB"
                     /locus_tag="RD477_001178"
     CDS             13640..15070
                     /gene="gatB"
                     /locus_tag="RD477_001178"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDT7633553.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            15080..16135
                     /locus_tag="RD477_001179"
     CDS             15080..16135
                     /locus_tag="RD477_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDT7633554.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEARRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRVIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            16246..17619
                     /gene="rlmD"
                     /locus_tag="RD477_001180"
     CDS             16246..17619
                     /gene="rlmD"
                     /locus_tag="RD477_001180"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDT7633555.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRTGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGVKPSVIVVDPPRKGL
                     DHRFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            17622..17750
                     /locus_tag="RD477_001181"
     CDS             17622..17750
                     /locus_tag="RD477_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDT7633556.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            18191..18379
                     /locus_tag="RD477_001182"
                     /pseudo
     CDS             18191..18379
                     /locus_tag="RD477_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            18457..19470
                     /locus_tag="RD477_001183"
     CDS             18457..19470
                     /locus_tag="RD477_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7633557.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(19537..20571)
                     /locus_tag="RD477_001184"
     CDS             complement(19537..20571)
                     /locus_tag="RD477_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633558.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            20685..22910
                     /locus_tag="RD477_001185"
     CDS             20685..22910
                     /locus_tag="RD477_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDT7633559.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALIHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHSDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLITDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22928..24811
                     /locus_tag="RD477_001186"
     CDS             22928..24811
                     /locus_tag="RD477_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDT7633560.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGTWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSTAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGYFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VYLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPEGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            24792..25766
                     /locus_tag="RD477_001187"
     CDS             24792..25766
                     /locus_tag="RD477_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDT7633561.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            26425..27447
                     /gene="aroF"
                     /locus_tag="RD477_001188"
     CDS             26425..27447
                     /gene="aroF"
                     /locus_tag="RD477_001188"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDT7633562.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGTQSLNEKTYSRMMKEVAIMKEAME
                     KIQRLD"
     gene            27480..28544
                     /gene="aroB"
                     /locus_tag="RD477_001189"
     CDS             27480..28544
                     /gene="aroB"
                     /locus_tag="RD477_001189"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDT7633563.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            28546..29712
                     /gene="aroC"
                     /locus_tag="RD477_001190"
     CDS             28546..29712
                     /gene="aroC"
                     /locus_tag="RD477_001190"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDT7633564.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRAAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSQVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSIDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            29729..30820
                     /locus_tag="RD477_001191"
     CDS             29729..30820
                     /locus_tag="RD477_001191"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDT7633565.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERTNLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            30833..32128
                     /gene="aroA"
                     /locus_tag="RD477_001192"
     CDS             30833..32128
                     /gene="aroA"
                     /locus_tag="RD477_001192"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDT7633566.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     THTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLQGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            32121..32630
                     /locus_tag="RD477_001193"
     CDS             32121..32630
                     /locus_tag="RD477_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDT7633567.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQRLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            32627..33463
                     /gene="pheA"
                     /locus_tag="RD477_001194"
     CDS             32627..33463
                     /gene="pheA"
                     /locus_tag="RD477_001194"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDT7633568.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPACLRALFRKQV
                     DLAVVPVENSLEGSVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIIGDRKITSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(33526..35262)
                     /locus_tag="RD477_001195"
     CDS             complement(33526..35262)
                     /locus_tag="RD477_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDT7633569.1"
                     /translation="MRKKIKEHELFTKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVESYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(35259..36767)
                     /locus_tag="RD477_001196"
                     /pseudo
     CDS             complement(35259..36767)
                     /locus_tag="RD477_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292017.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            37040..38326
                     /locus_tag="RD477_001197"
     CDS             37040..38326
                     /locus_tag="RD477_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7633570.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADNEEKVTVWAWDET
                     FNTKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFADLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMDNLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQVNASLKYGLELFATILKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            38463..39332
                     /locus_tag="RD477_001198"
     CDS             38463..39332
                     /locus_tag="RD477_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633571.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQWMLTDATFKKAFFNTFLYLLVQVPIMLFLALFICNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWAKVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLRLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            39329..40215
                     /locus_tag="RD477_001199"
                     /pseudo
     CDS             39329..40215
                     /locus_tag="RD477_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            40383..41066
                     /locus_tag="RD477_001200"
     CDS             40383..41066
                     /locus_tag="RD477_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDT7633572.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            41063..41836
                     /locus_tag="RD477_001201"
     CDS             41063..41836
                     /locus_tag="RD477_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDT7633573.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGFPYNYILVPLGFLMGFLVGF
                     AEPAIHVMVKQVEELSEGRIRSKVMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            41833..42231
                     /locus_tag="RD477_001202"
     CDS             41833..42231
                     /locus_tag="RD477_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDT7633574.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENKDREKSES"
     gene            42416..43087
                     /locus_tag="RD477_001203"
     CDS             42416..43087
                     /locus_tag="RD477_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633575.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHSSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            43146..43586
                     /locus_tag="RD477_001204"
     CDS             43146..43586
                     /locus_tag="RD477_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633576.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQITNKKQ
                     AKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            43598..44266
                     /locus_tag="RD477_001205"
     CDS             43598..44266
                     /locus_tag="RD477_001205"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDT7633577.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYASVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKKWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            44330..44953
                     /locus_tag="RD477_001206"
     CDS             44330..44953
                     /locus_tag="RD477_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDT7633578.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAKEFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            45009..45395
                     /locus_tag="RD477_001207"
     CDS             45009..45395
                     /locus_tag="RD477_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDT7633579.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            45608..46999
                     /locus_tag="RD477_001208"
     CDS             45608..46999
                     /locus_tag="RD477_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDT7633580.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            47211..47669
                     /gene="tnpA"
                     /locus_tag="RD477_001209"
                     /pseudo
     CDS             47211..47669
                     /gene="tnpA"
                     /locus_tag="RD477_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT        15723 a         8557 c        10392 g        13450 t
ORIGIN      
        1 aaaagtttgg ctggtaagtc tttttttgag cttaacttac ttttgtttct tgtctttttt
       61 cttttcattt tgtggctgtg gttttagaaa tcgatcgatc tcttcttttg gtgcaactgt
      121 tttttccgga tcgacagcaa atgattcgaa aagtttatct aaattatctg tacgttcaaa
      181 tttcaacccc attgtaaaca cctctttgtt tctttttact tagtctatca tgtaatcgtc
      241 caaattggtt tctaagacat aaagttttag tttttcttta tcgttggtta taattaaatt
      301 ataaaaagat tttaaagagg tgagaaaatg attggcgaag ataaattatt gatgactatc
      361 cagctcacag acttaaatga agaagaaaaa aaaacaggaa ataagcgatt gggctatttt
      421 gacacgtctg ttgatccagc caacatttat ccgctctttc acacaacaag cggatccgta
      481 tgatcttcga aagcttcaag aaaaaataat gctagcatcg gtaagagacg aaagttggtt
      541 agttgtagga aatgacgaga acgaatgtag ttttcgcttg gaagataatc aattattgat
      601 aaaaaatgta ttgtctatct ctgttttcaa agaaaagcaa tttttgattc gagacttcat
      661 tcagaaaaaa atgattgctc atggggtgtt cgcctatatg cgagcttata gcgagtttat
      721 ctatcacaat acaaaacaga tctctcaacg gctattgttt gagaaaaagg atgaaatcga
      781 acatttacct aaaatgaaac aacaaaacgg ggaagtagta gtcgattgca accaatttcc
      841 aggatacgat cttttttacc aaggtttatg tttcacttcg tgttgggaaa tgtattatag
      901 tcgctattat catcaaatca tccctaaacc gatttttctc gacatccaac aagtagaaaa
      961 agtgaaggaa ttagaaaacg aagtcatatg tatccagctt tatcgagacc cctttaactg
     1021 gcaaaagcca aacaatcaaa tgtttcaatc ctattttagg gaccagcttg ggtttgatca
     1081 tcttgcttgg gacaatggtg tcggtttatt gaaaccgcca ttcgttgaat acatttacac
     1141 tgaccatacg atccaatctg tccagtatca gaatgcacaa atgcaacctg ttcctaaaaa
     1201 gaatgcttca ttttttgtaa caaaaagtta tgatattcaa caaggagatt ataaagaaag
     1261 gcgtgttcgc ggaacactaa atgctcaggc gtattttcct tgggttgacg agaatcgctc
     1321 gcgcatgatg tgctataaag ttatcgatcc gactgttgct ttggataatg gaatcgaagc
     1381 ttattgttat tatattaggg aatatctaga agtagaggta acagatgaaa aatatcaaga
     1441 gtatctttta tccttaagaa tctatgttcc ttcagaatcc ctgtcagaat taccgttaaa
     1501 ggaaatcaaa catagactct ctgatgttac cttcaaacga ataaagaaaa agcggcgaag
     1561 tatccaattc gatgtcaaga aaggtgagca gcatctgcgt gtgcaatttt tagattaccg
     1621 agaattagaa caattgatta ctttacaaaa aatatgaggt gaagaaaatg gataaacaac
     1681 tctattatcg aataatggtt ccggcatcat tattatccgt ctgtctcttt tttgttccta
     1741 attttcctat tcgatcagtc atcggttttt tggtcatgtt tggcgggatg ggactttatt
     1801 attatcttct tcataaggaa agaaaacctg cttctgttta tatgaaaaaa acaagaagcg
     1861 aaaataaaaa acgataaaac ggcttgatga tagacaaaaa aataaaaaac cagtcttttt
     1921 gactggtttt ttattttttt ttgactgatt tataattttt atgaacgttt ttgactgatt
     1981 tatattgcga gtagaaaaaa aatatgctat tatttgtatt gaggtgatcg tgaaaatgct
     2041 tacagaggaa cgtagacaaa aaatcctaga cttgcttgaa cagcagaaaa tcgtgaaatc
     2101 acaagatctt gtaaaccttt ttaatgcttc tgaatcaacg ataagacgtg atttacaaga
     2161 gctggagaat agtgggtttc tagaaagagt tcatggcgga gctaagaaag agcaattatt
     2221 gaattttgag cagaatatga gtgaaaaatc actcaaaaac gttcacgaaa aacagcaaat
     2281 cgccaaatcg gcagctgaaa ccattaatga cgaagatgtc atttatttag acgctggctc
     2341 tactacgtta gaaatgattc cttttctgca aggaaaacag atcacagtag tgacaaattc
     2401 cgttcaacat gcggcaaatt tagttgattt ggagcttgct accattattt taggtggaac
     2461 gataaagctg gcaactaatg ctgttttagg atctaatgcg atagaacaac taaaaaattt
     2521 tcattttaat aaagcattta tgggaatgaa cggtgcccat cttgaacaag gattcacgac
     2581 tccggaccca gaagaggcag ctgttaaacg tttagccatg aaaaattccg aagagacatt
     2641 tgtattgatt gatcatacca aattcaacca gttgtccttc acctctgttg cacctttgga
     2701 agcagccacg atcataaccg agcgttgtcc attagaattc gttaatgaat tccgtgaaaa
     2761 gacaacaatt aaggaggcaa tacaatgatt tacacagtaa cacttaatcc atcaattgat
     2821 tatatcgtac gcgtagatgg gttaaaacta ggcgatttga accgaatgaa ggaagacttc
     2881 aagttacctg gaggaaaagg gatcaatgtt tcccgtattt tgaaacggat cgattctcaa
     2941 tctacagcac tagggttttt aggagggttc actggcgaat ttatttcgaa gtggcttgaa
     3001 aaagaacaaa tccagtcttt gttcacaacc gtaaaagaag atacaagaat caatatcaaa
     3061 ctaaaatcag atactgaaac tgaaattaac ggtttgggcc catctatttc agatgaagaa
     3121 atcgatgagt tgaagcaagc attaggaaca gtaggacaag gagatatcgt cgtattgtca
     3181 ggaagcgcgc cggctagcct gcgaaaagga ttttatcaag aactgattga tatcattcga
     3241 gaaaaaggtg cagatttcgt gattgataca acaggtgatg acctaaaaaa tgcacttgtt
     3301 aagaaaccat tgttgatcaa accaaacaat cacgaattag ctgagcttta caatacaacc
     3361 ttccaatctg tagaagaaat cattccgtat ggtaaaaaat tgctagctga aggggcacag
     3421 aatgtgctga tctcaatggc tggagacggt gctttgctct tcacggaaca aggaacttat
     3481 aaaacgaatg tattagttcg cccattgaaa aattcagtag gtgcagggga ttcaatgatt
     3541 gccggcttta ttggggctta cagccgtacg aaagatccaa tcgaagcctt taaatgggga
     3601 gttgcttgcg gaagcgcaac agctttttct gatgacttgg cagctcgcga attgattgat
     3661 gagttgctgc cagaagttga aatcacaaaa attgattagt ttcaaggagg ataatcatga
     3721 atatcaagga tttagtggtc aaagaagcga tgatcatgga tttgcaagcg actgataaaa
     3781 aaggcgcaat cgatgaaatg gtccaaaaaa tgtttgatgc cggccggatt tccgatatcg
     3841 aaacatataa agaaggaatt ctagcccgcg aagcacaaac ttctacaggt cttggtgatg
     3901 gaatcgccat gcctcacgcg aaaaacagtg cagttaaaga agcaacagtt ttattcgcaa
     3961 aaagccaaaa aggagtagat tatgaagcct tagacggaca gccaacttat ctgtttttca
     4021 tgatcgcagc tccagaaggc gcaaacgaca cacatttgca agcattagca gcactatctc
     4081 gattgctgat tgatgcagat ttcgtaggaa aattaaaaga agcaaataca ccagaagaag
     4141 tccaagactt gttccaaaca gctgaagagc aaaaagaagc agaagaaaaa gcagaacaag
     4201 aaacacaagc tgcagaagct tctgctcatc cagaaagaaa attcgttgtt gccgttacag
     4261 cttgtccaac agggatcgct cacacttaca tggcagaaga tgctctgaag aaaaaagcga
     4321 aagaaatggg tgtggagatc aaagtcgaaa cgaacggttc agaaggaatc aaaaaccgat
     4381 tgactgctga agacatcgcc cgtgcagaag gtgtaatcgt tgcagcagac aaaaaagtag
     4441 agatgaatcg ctttgacgga aaagaactga tcaatcgtcc tgtcagtgat ggtatccgta
     4501 aaccagaaga gctgatcaat ctagccctta gtggtactgc accagtattc catggtgatg
     4561 gaaaagaaag cagttcagaa gaaggaaatg cggatggtac tatcggacaa agaatttaca
     4621 aagatttgat gaacggcgta tcacacatgt tacctttcgt tattggtgga ggaatcgcta
     4681 ttgcgctttc tttcatgatc gaccaattta ttggcgtgcc acatgatcaa ttatcaaatc
     4741 tagggaatta caatcaagct gctagctggt tcaaccaaat cggacaagct gcatttggtt
     4801 tcatgctgcc ggtattagca ggatttattg cttcaagtat cggtgaccgc cctggtttga
     4861 tcgttggatt tgctgctggt gcattagcta atacaggcgg tgcaggattc ttaggtgcac
     4921 tgattggtgg attcttagct ggttatgtca tcgtattttt acgtaaatta ttcaagaact
     4981 taccaaaatc attggatgga attaaaacga tcctctttta tccagtgttt ggcttactga
     5041 ttactggttt cttgatgttg ctggtcaatg tccctatgaa agcaatcaat gatgcattga
     5101 acggtttctt gacaagtatg agtggttcga atgctgcttt acttggtgca ttattagctg
     5161 gaatgatggc ggcagattta ggcggtccaa tcaacaaagc cgcttatgtg tttggtacag
     5221 caactttagc tacgactgtt gcaactggcg gaagtgtcgt gatggcttct gttatggctg
     5281 gaggaatggt acctccattg gcaattttcg ttgctacacg tttgtttaag aataaattca
     5341 gcaaaacaga tcaagatgct ggtttgacga acatcgtgat gggactatcg tttgtcacgg
     5401 aaggatcgat tccatttgca gcagctgacc cgattcgtgc cattccaagt tttattattg
     5461 gttctgcttt aactggcgga ttagtcggag ctttcgggat caaattactg gctcctcatg
     5521 gcgggatttt cgttgtattc ttactgagtc acccggtcat gtatttggtg tttattgcta
     5581 tcggagcagt cgtatctggt gtgatttatg gtttattgag aaaagctcct gcagcagcag
     5641 aagcatagaa ataaaaaaga agaggctggt ttttcccagt ctcttctttt tttatagaat
     5701 catatagaat caagtagtgt agatgaatag ttgttgtaaa atagtttttc catgttcgtt
     5761 aaaatgattg cttcaaattt gtgatagaag atttagcctc gatttcctgc ttgattcttg
     5821 gcaagtttgc ttcatcagtc aagataacca aaatatctcc tacattcatg atcgtatcac
     5881 cgtgagtcaa gatttcctga tttcctctgc gtatagagat tagcagcata tttttaggcc
     5941 aattgaagtc tcgtaccatt aatccgtcta aagaactctc tgccgttact ggaaaatcaa
     6001 aagttgtcct tgcacctttt agtgaggcgg tctgagtatt tccaagtaga cgttccaata
     6061 atgattcgta aattggatta ccgccgagaa ggtcattgac gatataagca acaagagcag
     6121 ccaatcctaa aggcatcaga tgattaagag agccaaccat ttctgtcact aaaataattg
     6181 ctgtcagcgg tgcttttcca atcgctgtga aataaccagc catggcaata acgacaaaat
     6241 ccttgatgta gattggatcc atcccgaaat caaccataat gtttccataa aagctaccga
     6301 ttaaagcccc caatgtcaat atcggaagga aaatcccccc tggaagacca ccaccgtaag
     6361 aaatcatgga aaaaacgaat cgaagaacaa acaaaccgat aatcataatg attggcaagg
     6421 aagcatgtcc aaaagctaaa acgatttgat tgcctccgcc tagagtacta ggccataaat
     6481 aaccaattgg caaaatcagc aagaaaggaa cgaacccgtg aaaataagct ggtaaaggca
     6541 atcggttgta aaattttggc aaagccagca agaccttctg ataaataaaa cctaagatgc
     6601 ctagaagtac tcctaagatc actaaggaac cataatattt caacggcaaa gaaggaaggt
     6661 cacctagata taaaacaggt ctcaagccga aaaagtaaag agagacgaag ttagcagtga
     6721 ttgctgaagc aaatgatgtc agccaaacaa gtggagaaaa ggagtgatgg atttcttcta
     6781 aaacaaagag taatcctgcg ataggagcat tgaatgctgc tgcaagtccg ctacttgcac
     6841 cgctagaaat taaaatcttt tcttctgaag atggactccg aaacaattgt ccagctcctt
     6901 gtccaatgga tgcgccaagc tgtatagaag gtccttctct acctagaaaa aggcccgcac
     6961 caactgaaag tacaccgcca atgaactttt tccataaaac agagaaccag ttcattgata
     7021 attccccgcg aagctgtccc tctacttgtg gaatcccgct tcccttgata tccggttcat
     7081 ttttgaccag ataacccacg aaaaatgctg caaggatgca gcaaatagct aggccaatta
     7141 agtaaatcgg ctgttcccga ctcatttcat agatacttcg aactaaatcg cttagctttt
     7201 cgatagataa tcgaaacagg ctaacgacga ttcctgtcag cagtccaaca aatattcctt
     7261 ttagaataaa atatatctga gtcttatcta atttcttgat ttttcctttt ttttccattc
     7321 aaacgcctcg ctttctttta atttagcgcg aaatcaacga attgacaagg aaaaatccat
     7381 aaaaagaaac aaatgatttt gaactagcta gtttaatgaa agccaagaaa atgctataat
     7441 taacaagatt gataaaagaa aggatgttgt taatgtcatc caaagctgaa ttattaaacg
     7501 ggatgaaccc ccgtcagaaa gaagctgttc ttcacactga cggaccatta ctgctaatgg
     7561 caggtgcagg aagtggaaag acgagagtgc tgacacaccg cattgcctat ttgattgagg
     7621 aaaaagaagt caatccttgg aacatcctag cgattacttt taccaataag gctgcaaaag
     7681 aaatgaaaga acgtgtcaat gcgattctcg catctggagg cgaagatgtc tgggtatcga
     7741 cttttcactc gatgtgtgtg cgtattttaa gaagagatgt tgattttatc gggtataacc
     7801 ggaactttac aatcatcgat tcttctgaac agctgacctt gatgaaacgg atactaaaag
     7861 aactgaacat tgatccgaaa aaatatgatc ctcgaagtat attaggaaca atctctcaag
     7921 caaaaaacag cctgcagacc cctcaggatt ttgcaaaaat gcaaggcagt tattacgaag
     7981 agattgcggc aaaatgttat gctgcttacc aaaaagagct tcaatataat caatgtatgg
     8041 actttgacga cctgatcatg aatacgattc gattatttga agaacatcct gattcactca
     8101 catattatca aaacaaattc cattatattc atgtagatga gtaccaagat acaaatcacg
     8161 cacagtatac gctagtcaat ttattggcag gtagatttag gaacctgtgc gtcgtgggag
     8221 atgcagatca aagtatttac ggctggcgcg gtgcagatat gcagaacatc ttggattttg
     8281 aaaaagatta tccagatgct gcagtaattt tgctagaaca aaactatcgc tccacgaaaa
     8341 acatccttag cgcagcaaac caagtcattg aaaacaatag caatcgaaaa ccaaaaaact
     8401 tgtggacaga aaacaaagaa ggaaacaaga tcacttacta ccgtgcagat aatgaacgag
     8461 acgaaacccg ttttatcgta gatcggatgc aagaagagat tcgaagcaat catcggaatt
     8521 acggcgactt tgccatcttg taccggacga acgcacaatc tcgtgtaatg gaagaaactc
     8581 tgttgaaagc aaatattcct tataaaatgg ttggagggca caagttctac gatcgtaaag
     8641 aaatcaaaga tattttagcg tatttgaatg tgctggcaaa tcctcaagat tcaatcagtt
     8701 ttgagcgtat cgttaattcg cccaaacgag gcatcggacc aggatctatc gaaaaactac
     8761 gcagttttgc ttctcttcat gaatggccac tgcttgaagc agcgcaaaat gtggatctgg
     8821 caaatatcgg tggaaaagca ggacagcaat taggtgcatt tggagagatg atccaagaag
     8881 tcactcagat gatcccatac ttgacagtga cagaattgac gaaagaggtt ttggatcgta
     8941 gcggatatct agaagactta aaaatccaaa atacattaga agcacaagca cgcatcgaaa
     9001 acttggagga atttttaacg gtcacacaag aattcgacaa acaattcgaa cagcaaaatg
     9061 aagaagacgc tgatgcacca gaagaaaaac taacggtctt cttaaatgat ctggcacttg
     9121 tttccgatat tgacaattta gaagaagatg cttcccaagt gacattgatg actttgcatg
     9181 cagccaaagg attagaattc ccagttgtat tcctgattgg tctagaagag ggggtttttc
     9241 ctttatctcg agcattgatg gaagaaagcg aactggaaga agaacgccgt ctcgcatatg
     9301 tggggatcac gcgagcagaa gaagcgttgt atttaacaaa tgctttctcc agaacattat
     9361 atggacggac gcaatataac cgaccttctc gttttgtaga agaaatcgat caggagttac
     9421 tggaaatcga aggcatgcgt cctacgccta aaaaaacacc tgtattcgct aaaaaaacag
     9481 catatagcta taaacagcca gagacagcgg ttgttccctc taaaagtgct actggagggg
     9541 aaaataacag ctggaaacct ggagataaag tcaaacacaa aaaatggggt ctaggaacag
     9601 tcgtccgagt aagcgggacg tcgaaggatc tagaactaga tgtggctttc ccaagtcaag
     9661 gagtaaaacg tcttttggct gcctttgcgc caattgaaaa agcttagtag tggagggtaa
     9721 atgatggaac atgaaatgac gattgacgaa gctgctaaac gagcagaaga acttcgtaca
     9781 agactgaacc aatggtctcg cgaatactac gtagaagata aaccaacagt ggaagactat
     9841 gtttatgata aagaatacgc agaattagta gcaattgagg aacaatatcc tgatttgatc
     9901 acgtctgatt ctcccactca acgagtaggc ggaaaagtat tagaaggttt tgaaaaagtc
     9961 actcatgata ttccgcttta cagtttgaac gacgttttca gcaaagaaga gctgatagct
    10021 tttgatcaac gtgtacaaaa agcagtggga cgtgtagttg actattgctg tgaactaaaa
    10081 atagatggac tttccgtttc tttgcgttat gaagatggaa atttcgtgag aggtgcgact
    10141 cgcggggatg gaactgtagg agaaaacatc actgaaaacc taaaaactgt ccggtcggtc
    10201 ccaatcaaat taaaagaacc aatgaacatc gaagtaagag gcgaatgttt catgccaaaa
    10261 cggtcatttg tccaattgaa ccaagacaga gaagcagagg gaaaagatat ctttgcgaat
    10321 cctcggaacg ccgcagctgg ttctttgcgt cagttagatt ctaaaatcac agcgaaaaga
    10381 aatctggaca cttttttata cacagtagct gatttcggtc ctatgcaagc aaaaacccag
    10441 tatgatgcgc tagaagagct agaaaaaatt ggattccaca cgaatcggga aaaacgtcta
    10501 tgtcattcga ttgatgaagt gtggtcatac attgaagaat atcatgacaa acgtgtggat
    10561 ttgccctatg aaatcgatgg aatcgtcatc aaagtgaatg aattctcgct acaagatcaa
    10621 cttggtttta ctgtcaaagc accgagatgg gcagcagctt acaagtttcc gccagaagaa
    10681 gttgaaacgc taatcgaaaa catcgaatgg acagtcggtc gaacaggggt agtaacgcca
    10741 acagctatca tgacaccagt ccgagtagca ggaacgacag taagccgtgc cagcctgcat
    10801 aacggtgatt atattaagtt gaaggatatc cgcttgaaag atacagttct gatttataaa
    10861 gcgggagaca tcatcccaga agtttcgcaa gtcgtgttag ataaacgacc taaagacagc
    10921 gaagaatacc aattgcctac acattgtcca gtttgtggaa gtgaacttgt ccatttggat
    10981 gaagaagtag ccttacggtg catcaatcct aaatgccctg ctcaaatgaa agaaggtttg
    11041 aaccactttg tttcaagaaa tgcgatgaat atcgatgggt tggggccgcg cgtactagaa
    11101 cagatgtatg ataaaaaact agtagcagat gttgctgatc tttacaaatt gacagaagaa
    11161 gagttattga cgttggacaa aataaaagaa aaatcagcaa ataatatttt gacagcaatc
    11221 gacaacagca aagataattc ggtcgagcgg ttgattttcg ggttaggaat ccgacatgtc
    11281 ggagcgaaag cagcaaaaat tttagcagaa catttcggtg atctagaaac attgagtaaa
    11341 agtgactatg agtcgattat tgcgcttgat acgatcggtg atatcattgc cgacagtgta
    11401 gtcacttatt tcagtaacga agaagtccat gaattgatga atgaattaaa acaagcaggc
    11461 gtcaatttcg aatacaaagg gcttcgaaat gcccaattgc aagaagtcga gtctcctttt
    11521 aaagaaaaaa cagtagtcct tactggtaaa ctaacacgat ttactcgtga agaagcaaaa
    11581 gagacgatcg aaaatcttgg cgggaaagtg actggtagcg tatcgaaaaa aacggatatc
    11641 gtggtagcag gagaagatgc tggcagcaag ctgaccaaag cacaagaatt aggaatcgaa
    11701 gtttggacag aagatcaaat ggcagatgca ttggctaaaa gtcgctcagt agaagaatga
    11761 aaaatgagtt ttttcagaca aatttcaata aacaatttca gacacggcac tcttgtgtta
    11821 aaatagagag caaagttttt aactcgttta gaaagaaggg gtagtatggc gattagtgaa
    11881 gaacaagtga aacacgttgc caaattagca aaacttgcgt tcgcagatga agaacttgca
    11941 agttttaccg accaattagg taaaatcatc gatatggttg aaatgctgga agaagtagat
    12001 acagaaggtg ttccatttac gtcgaatgtg gttgaaacag taaacgtgat gcgacaagat
    12061 cgggcgcaag ctggatggaa ccgcgatgaa ttattgaaaa atgtgccgga aacagaagac
    12121 ggctttatca aagtacccgc aatcatcgat aatggggagg ctggcgcata atgacagaat
    12181 tacacaatcg ttctctagaa gaactgcatc atatgcttgt atcgaaagaa atcactgtac
    12241 aagatttgac aaaagcaaca tttgaacgaa tcaaagaaac agagcctgag gtcgaagcat
    12301 tcattacatt gaacgaagaa aaagcattgg aacaggcaaa agctttagat gaaaaaggga
    12361 ttgatgaaag caacgtcctt gctggtattc caattggtat caaggataat atcgtaacaa
    12421 aagatatctt gacaaccgct gcatctaaga tcctttacaa ttttgaacca atttatgatg
    12481 caacggtcat ggataaaatc tatcaagcag atatgatccc tgttggtaag ctaaacatgg
    12541 atgaatttgc tatgggtgga agtacagaaa cttcttattt caagaaaaca aaaaatgctt
    12601 gggacccaac aaaagtacct ggtggctctt ctggtggttc tgcagcagca gttgcagctg
    12661 gacaaattcc agtttcttta ggtagtgata ctgggggaag tatccgccaa ccagcagctt
    12721 ttaacggcat cgtagggatg aaacctactt atggccgtgt ctcacgtttt ggtttgattg
    12781 cctttgcttc atctcttgac caaatcggac caatgacccg tacggtgaaa gataatgcat
    12841 tagcattgac agctatcagc ggttatgacg aaaaagacgg tacttcttca ggcgtttcag
    12901 ttcctgattt tgcagaggga ctgacaggcg acatcaaagg aatgaagatt gctttaccta
    12961 aagaatactt aggcgaaggt gtggcccctg gcgtaaaaga agctgtattg aaagcagcag
    13021 aaacattccg ttcattaggt gctgttgtgg aagaagtcag cttgcctcat tcaaaatacg
    13081 gtgtggctgt ttattatatc attgcctcat cagaagcaag ttccaacttg caacgattcg
    13141 atggtatccg ctacggttat cgctcagaaa atgtgaaatc attagatgat gtatatgtca
    13201 actcacgttc agaaggattc ggtgaagaag taaaacgccg tatcatgtta ggtacattct
    13261 ccttaagtgc tggttattat gacgcttact tcaaaaaagc gggacaagtc cgtacattga
    13321 tcaaacgaga ttttgaaaaa gtctttgcag attatgactt gatcatcggg ccatcttcac
    13381 caacagttgc attcggaatc ggtgagaaca ttaacgatcc aatcacaatg tacatgagtg
    13441 acatcttaac gatccctgtc aacttggctg gacttccagg tatgtctgta ccggcaggtt
    13501 tatcagaagg attaccagtt ggattgcaga tcatcggtaa ttattttgat gaaaaaacga
    13561 tgtatcaagc ggcatatgcg tttgaacaag caacagaatt ccataaacaa caaccagcta
    13621 ttttaggagg gaaagacgaa tgaattttga aacagtcatt ggactagaag tccacgtcga
    13681 gttaaaaacc aactcaaaaa ttttctctcc agcgccagct cattttggtg cagaaccaaa
    13741 cagcaataca aacgtgatcg actggggtta tccaggcgtg ctgccagtaa tgaataaagc
    13801 tgcgctggaa ttcgggatga aagcagcttt ggcattaaac tgtgaaatct ctaaagatac
    13861 tcattttgac cggaaaaatt acttttaccc agataatcca aaagcatatc aaatttccca
    13921 atttgatcaa ccaatcggtc atgacggatg gatcgaaatc gaagtagaag gtaaaaagaa
    13981 aaaaatccgg atcgaacgtg tccatttaga agaagatgct ggaaaaaaca tgcatggtat
    14041 tggcggttat tcttacgttg atttgaaccg acaaggcacg cctttgatcg aaatcgtttc
    14101 tgaagctgat atgcgttcac cagaagaagc atacgcttat ctagaagcat tgcgatcaat
    14161 tatccaattt acagaagtca gcgatgtgaa aatggaagaa ggctctatgc gctgtgatgc
    14221 caatatttct cttcgccctt atggacaaga agagttcggt acgaaagcag aattgaaaaa
    14281 cttgaactct ttatctttcg tgaaaaaagg tttagctttt gaagaaaaac gtcaagcaaa
    14341 agttcttctt tcaggaggag aaatccaaca agaaacacgc cgttttgacg aaacaacgaa
    14401 taaaacagtt ttgatgcgtg tcaaagaagg gtctagtgat tatcgttact tcccagagcc
    14461 tgatatcccg aaattcgtga tcgatgacga atggattcaa aaagtaaaag ctagtttgcc
    14521 tgagatgcca gcttctcgtc gcgaacgcta tatccgtgag tttggtcttc cagagtatga
    14581 cgcaatggta ttgacactta caaaagaaat gtctgatttc tttgaagctg ttttggaaaa
    14641 tggtgcagat gccaagcaag catctaactg gttgatgggt gaagtttctg cttacttgaa
    14701 cagtgagaaa ctggaattag ctgagacaaa attgacacct gagaacttag caggaatgat
    14761 tcgtttgatc gccgatggaa cgatcagctc aaaaatcgcc aaaaaagtct tccgtgaatt
    14821 gatcacaaat ggcggagaag cccaagaagt agttgaaaaa aatggttggg tgcaattatc
    14881 tgatccgaac aaactactgc caatcattaa tgaaattttg gataacaacc aacaatctgt
    14941 cgatgatttc aaaaatggaa aagatcgtgc ggttggcttc ctagtcggtc aaatcatgaa
    15001 agcaactaaa ggacaagcaa atcctggtgt cgtgaataag ctacttaaag atgaattagc
    15061 gaaacgatag aggctgaaaa tgaaaaaagc acgcgtaatt tataacccca cctcgggaaa
    15121 agaattattg aaaaaaaatt tggcggatat cctacaggca ttagaagaag caggatttga
    15181 agccagcgcc tatgcaacta caccagaacc agattcagcc aagaatgaag cacgtagagt
    15241 cgcagaacta ggttttgatc tagtagtggc agctggtggc gatggcacaa tcaacgaagt
    15301 ggttaatgga atcgctccgc ttgaacaccg tcctaaaatg gccatcatcc cagctggtac
    15361 aacgaacgac tatgctcgag cattaaaaat tcctagagat aatattaaag cagcagcaga
    15421 agtgattaaa aagcatcaaa ccgtaaaaat ggacatcggc ctttctgata acagttactt
    15481 catcaatatc gctgcaggtg gctatttgac agaattgact tatgaagttc catcagaact
    15541 aaagagtatt tttggttatc ttgcttatct tgcaaaagga gcagagatgc tccctcgagt
    15601 aaagcccatc aaaatgcgct tgaagtatga tgaaggggaa tatatcggaa atgcttcgat
    15661 gtttttccta gggctgacga actctgttgg aggattcgaa cagatcgcgc cagatgccaa
    15721 attggatgat gggaagtttt ctttaatcat tgtgaagaca gcaaatatct ttgagatctt
    15781 gcatattgcg gcactcatgc tgaatggcgg tcgacatgtc gatgatccaa gagtaatcta
    15841 taccaaaaca agcagtttgt tcgtcgaaac agaagatgat atcaatcgac cagtaatgat
    15901 caacttagat ggtgaatatg gtggggaagc accgatgcat ttccaaaatc ttcatcagca
    15961 tattgagttc tttgccaata ccgatcagat tcctgatgaa gcaatcacag gcacagatga
    16021 agaagaatta gaggttgtta gcaaagaatt tgtaaaagaa gtcgaacgtt taacggatga
    16081 agatatcgac ggtgacggaa aaatagcaga cgaaacacct aatcaaaaga attaatcaaa
    16141 agccggtgct gaacgtagta aggtcagtgc cggttttagt tgtgtaatta gctgatttat
    16201 agtagagttc agtaggattg aaaaataggc ttaaaggagc aaaatatgac agaacagcaa
    16261 atagtaaaga aaaacgaacg atatacagtc gaaatcgtcg acttgagtta tgaaggaatg
    16321 ggggtagcaa aaatcgatgg ttatccatta tttatcgaaa atgcactccc tggagaagaa
    16381 gtagaaatac tagtagtaaa agcaggcaat aagtttggat atggaaaagt cgaaacattt
    16441 attacacaat cacctgatcg tcagcctgta gaaaacaatg cattacttcg cacaggtatc
    16501 gcacctttag cccatttgaa gtatgaacaa cagcttgtct tcaaacaaaa acaagtagag
    16561 catgtaatgg cgaagattgc taagatgcca gaagttcctg tacttccaac ggtcggaatg
    16621 gcagaaccat ttggctaccg taataaagca cagatccctg ttcgccgtat cgatggacag
    16681 ttagccaccg gtttttacaa aaagaatagc catgatctag tccctatcga agatttttat
    16741 atccaagatc ctgtcatcga tcaagcaatc aaagtagtca gagatatctt gcaacggttc
    16801 caagtacgag gatataatga agaaaaaaat gaaggacagc ttcgtcacat cattatccgt
    16861 cgtggtcatt attcccatga aatgatggtc gttttagtta ctcgtaaaga aaaattcttt
    16921 aaatccaagg aaattgctga agccattcat gaagaattgc cagaagtggt ttcggtcatc
    16981 caaaatatca atgaagaaaa aacaaatgtg attttaggag acaaagaaaa agttttatac
    17041 ggcagagact atatcgaaga tcagctatta ggaaaaacct atcgaatctc agcgaagtca
    17101 ttttatcaag taaatactca acaagcagag tatttgtatc aaacagccat cgaatttgcc
    17161 gagttggata aaaaagacat tgtgatcgat gcttattcag gaataggtac aatcggattg
    17221 tctctagctg acagagtaga taaggtatat gggatggaag ttattccaga agcaattgaa
    17281 gatgcacagt tcaatgcctt aaataataaa atcgaaaatg ttcattatga agtcggaaaa
    17341 gcagaaaaag tcatgaaaaa atggcaagaa aaaggggtca aaccatctgt gatcgtagta
    17401 gaccctccac gtaaaggatt agatcatcgt tttattgaat cagcaatcga aatgtcacca
    17461 gaacgaatcg tctatatctc ttgtaatcca gcaacttttg caagagatgc taagttattt
    17521 gcagaagcag gttacgccgt gaagaaagtt cagcctgtag atcttttccc gcaaacccat
    17581 catatcgaat tagtcgcttc attcgttaaa ggagaataaa aatggaccaa aaagaaaaaa
    17641 tgaaagaatt gatcaaaaag aaacaaggtg gaggccgatc caaacaaatg gaaacaccta
    17701 aaaatgatcg taaaaacatg agaaaaggcc ctaaaatctt taataaatag acgtgctaaa
    17761 gaaagactaa gaagcgttaa cttttgttat tgtttcttag tctttttagt tagtctacat
    17821 tattttggcg atttgccaat aaggactgtt gagttgtgca aaatcaaatc tgggtcagaa
    17881 tgaacgtcat tctgtctcag atttttcgtt atttcaattt gattaactga ttgatcaaaa
    17941 agatttttat tttcatattc tcaaatgatt caacactaaa tatacatatt tgacgcggaa
    18001 attattattc gatttgggcg gcatcagggg gttatgacga cgtagcagac ccattatttt
    18061 actcaagaaa atgtccgtgc ctactattta gaatacgata gtgaacgtgc tgggagcttt
    18121 gttcttttag agaaagtctc tgaagataaa ttagttgtct tagattttga ctgtaacgaa
    18181 aaatggcgaa atggaagagc accagcaagt gattgaccgg atctacgaag cgacacgata
    18241 tctgcctttg gatcgtttgt gattaagtcc tcaatgtgga tttgcttcaa cagaagaaga
    18301 aaatgttttg tttgaagaac aatggaaaaa atttcttttg tcaaaagcat tgcagaagaa
    18361 gtttggggaa aatcattaaa tcatctagga gagcttgata ggaaataaca ctatggtata
    18421 atcagaatgc ttaatactaa ctactgaggg agccttatga aaaagaaaat tcttaaattt
    18481 atcggatggt tcttcggaat cattcttggg atcgcgtttg caatctttct tgccttccag
    18541 ctttccccca gacctggagc attcttgatc aaccatatgt tttcaaatac tgttcaaatc
    18601 actgataaaa aaacgttcga tcaagcagaa aaaaacgtgg cgaaaaaaac agatttgacg
    18661 tatgagtcca agtataaaga gaatacttat gacatctact atccaaaaaa cagcaagggg
    18721 cctgttcctg ttctattttg ggtacatggt ggaggattcg tcggcggaga taaatcgggt
    18781 gtcaaagaat ttgcgacaaa attagtttca gacgccaaca tcgcagtggt tgcgatgaat
    18841 tatgaattag cgcctgattc agaataccct aatcaagttc ttcaagtgaa tgaactgatt
    18901 aaagatttat tagctgaaga aaaagacgac aaattgctag acatggaaca attatttttt
    18961 ggtggagata gtgcaggttc acaaatcgca ttgcaatatg cagctataca aacgaatgac
    19021 gcgtatgcta aacaaatgaa aatcaaacag ctacttccaa aagatagtct gaaaggaacc
    19081 atttcttatt gcggacctgt tgatttgaag ctaacagcac agcagcattc ggatgatcga
    19141 tttatgaagt ttttcgtcaa aaccgttgcg tggtcgttga ttggaacaaa agattggaaa
    19201 acgagtgagc aattgaaaga agctagtgtg gctgaccatg tttctaagga atttccgcca
    19261 acgtatataa ctgacggaaa tgcatactca ttccaggagc aaggtcaagc attagaaaat
    19321 cggttgacag aattaaaggt acccgtgcaa tccttgtttt atacagatac aaaaaaagag
    19381 attacccatg aataccaatt tgattacacg ctaaaagaat cgcaaaattg ttaccagcag
    19441 acattggatt ttgtgaataa gtataagtaa gaggttgtga aaaagccgtt tcgttttgag
    19501 tatgaagaaa cgaagcggct tttcacaacc tctctttcaa tacctttttg ttcttgggat
    19561 aaaactttcc ctaacaatca atcgtgcagg ataagtcaaa atgattggtt cgtcccgtat
    19621 accgtcaatt cgctccaatg ccattcgaac agctgcttgc ccaattgatt tcgcattgat
    19681 ttgaacactg gtcaagccag gggagaggtt tgcgattgtc tcgatattat caaagcttgc
    19741 tacaacaatc tcttctccaa tttggatatt tttttgtttc aatgcccgat agacaccaat
    19801 tgccattgga tcgctcccga caagaatggc agtaggaaga ggggattcat tttctaatag
    19861 attagtcgca agtacctcac cttgtttttc tgtccagcca cctagatgat aagacatata
    19921 tttttctaag tgatgatcga tcataaaatg ctgataggca cgcatcctct tttcatttat
    19981 agaaatctgc ttcccaccga attcatccat atcgacttta tatcccccga tataggctat
    20041 tcttttatga ccattttcta agaacaattc cagtacggtt ttagtcatcc gttcaagatc
    20101 tgcatacacc atatcgacct cttgctggat gtcagggtta tccacgacga ctatttgcat
    20161 gttttgtttt aacaaggcgg taatcgattc tttcgtaaca gtcccaacaa cgatcaccgc
    20221 acctaactga tctagatctt cccattgttt aggattatca gatagattat agattcgatt
    20281 catccctaaa tgcagacgtc gagcttcttc ttccatccct actcgcaaat gtttgtaata
    20341 gatatcctgt tcttcgctcg ctttgccgat cgtagagatc acaccaatcg aaggcattct
    20401 ctttttgata tatttacgag tactgatgtc ataatccaac gcagcagctg cctctactat
    20461 ccgttgccga gtagtctgag ccacatggat cgtctgatcg ttattcaaca cgcgggaaac
    20521 tgtagcagga gagacatttg cctttcgtgc gacatctcta attccaacca tttttacaca
    20581 ctcctcaaaa aaataaaaaa tttttattga caaaattgtt tagtaaacag tatacaggaa
    20641 ttataaaaaa agtaaacggt ttccgttttg ttgggagtga aatgatggat attacgcata
    20701 gaaaaacaga taaaaatttc atcttatttg atgaaaagag ccgagtcttc catttacgaa
    20761 atacatttct ttcttatctg ataaagatag aagaaagcaa tgtattggcg catatttatt
    20821 ttggcaaacc agtcaagcag tacaaagaca ataaaaatta tccccgaaga gatcgtggat
    20881 tttcaggaaa tgttccctta aatcctgacc ggtctctgtc gaaagataca ctaccgcaag
    20941 aatactcttc tcatggcagc atggactttc gaacgcccgc atcgatcatt cagcgcaaga
    21001 atggatcaga cttactggat ctgagatatg acagccatta tattacagat gggaagccag
    21061 agatcgaagg cttgcctcaa acttatgtga tggacaagtc agaggcacaa acgttggtga
    21121 tttcacttaa ggatagagaa acagcgattt atttcgattt gttctatacc atatttactg
    21181 atagagcagt gatcacacga tctgtaaaga taagaaatga aaccggagaa acaatcaaac
    21241 tagaaaaagc ggctagtttc cagctagact ttgcccatac taggcgattt gatgaagtca
    21301 ttgctttgcc aggagcacat gtcaatgaaa gacagatttc acggcaatct gttttatctg
    21361 gaacaaaagt atttgaaagc cgtcgaggaa cgagcagtca ccatatgaat aactttattg
    21421 ctttaattca tcatcatacg acagaaaata ctggagaagc gatcggactt caatttgttt
    21481 attccggcaa ccattctttc gaacttgaaa aagatcagat caatcaattg agagtcgtcg
    21541 gtgggatcaa cagccatcgt ttttcgtggg aattgaatgc aggccagtct tttcaaacac
    21601 ctgaaatgat cctttcttac tcaagtcagg gattgaacaa aatgagtcag atccaccatg
    21661 agttgctacg tgaaagaatc gcaagaggtc gtcatcaatt tgctgaacgg ccgattctgg
    21721 tcaataactg ggaagccact tactttgact tcaatagtga aaaaatcaag gcgatcattg
    21781 atgaagcaaa ggagctaggc atcgaaatgt tcgtattaga tgatggctgg tttggaaaaa
    21841 gagatgcgga caattcttca ttaggagact ggttcgagta tgaaggcaag ttgacaaatg
    21901 gattacgtga aatagcagat tatgctcata gcaaagggtt gaaatttggt ttatggtttg
    21961 aacctgaaat gatctcggtt gattctgaac tgtatcgaac acattctgat tttcttatgc
    22021 aggagccagg tagaatgcct tctgcttcca gatcacaaca tgtactggat tttacaaggt
    22081 tagatgttcg gcagacgatt gagaaacaaa tgagaaagat tttagatacg attcctcttg
    22141 actacatcaa atgggatatg aaccgttcgc tttcagatgt ctattcgatt acccttgacc
    22201 cacagagaca aggagaagta gcgcatcgtt atatgctcgg gctttatgaa ctacttgaac
    22261 atttgataac tgattatccg gaaatcttat gggaaggctg ttctggtgga ggtggacggt
    22321 ttgatgccgg gtttatttat tatatgccgc agtcatggac aagtgataac acggatgcag
    22381 tggagcgtat gaaaatccaa tatggaacaa gtcttgctta tccgatttcg tccatcacag
    22441 ctcatgtatc agctgtacca aatcatcaga ccgacagatc tacttcgttg aaaacaagag
    22501 gagaaacagc tatgagcgct gtttttggtt atgaactgga tttgacaaag ctgtctcctg
    22561 aagaaaaaaa gcaagtcaaa gaccaaatta tatcctatca aacgattcgt ccggtgatcc
    22621 agtatggcca ttattatcgt ttggcgagtc cttttgaaga aaatattgcc gcatggatgt
    22681 ttgtgtctcc taaacaagat gaagctattg tgtttttagg gaggatacta gcttcagctc
    22741 aaccagcgtt ccatgaagta tatctgatgg ggttagatga tgaggcactt tatcaggaac
    22801 agacctcgaa gcggatattt tcgggggccg aattgatgac agtcggactt tacttcccag
    22861 attttcaagg tgatttccaa acagagctgc ttcatttcaa aaagttatga gagagaagga
    22921 aaaaagtatg aaagcaaata taaaatggtt agatgatcca caagttttta gagtgaacca
    22981 atcaaatgca catagcgatc atgtttgcta tgcaacaagg gaagaagtag aaaccacatc
    23041 ctcccttaaa cagtctttaa atggaacatg gcgtttttgt tattcgccaa atccttcggt
    23101 acgtccaaaa acattttata aaaaagattt tgatgcctgt tcttttgatg aaatcactgt
    23161 tcctagtcat attgaattag caaattatga caagatccat tacatcaata cgatgtatcc
    23221 gtgggaggga catgtatttc gccgtccccc tttcacgaca agccctgaga aagaggcgga
    23281 agggatattt tcaactgcta aagacaatcc ggtgggttcg tatttgacga cttttcgatt
    23341 agaagaaggt ctaaagggaa aacaaatctc gattcatttt gctggtgtag aacaagcaat
    23401 gtttatttgg ttgaatggtt attttatcgg gtatgccgaa gattcgttca ctccgtctga
    23461 atttgattta acaccttatt tggaagaaga aaatattcta gctgtggagg tttacaaaag
    23521 aagtacagct gcattcttgg aagatcagga ctttttccga ttttttggga tcttcagaga
    23581 tgtctactta gttgctcgag gacaaaacca tttagaagat ctttggatca aacctcgatt
    23641 agatgacaaa tgtcaaaatg gcaaacttga agtagatttg tcttttgagc aagtagccaa
    23701 aaacagtcag ctgaatgtag cgatactaga tcctgagggg aaggaaatct atcaacagca
    23761 atatccgtta aaagagcaaa tgacgattcg gatcgatgga tttgaaaacg tggcactgtg
    23821 ggggcatttt cagcccaatt tatataagct tgaattggag acgatagacg aagaaaaaag
    23881 agtaacggag tatattgact attccttcgg ttttcgtaaa atcgaaatca aagataaaat
    23941 catctacttc aatggaaaac ggatgatcat caacggggta aatcgacatg agtggcatcc
    24001 aaaaaaagga cgcgccatca ctatagaaga tatggaaaaa gatttggcta tcataaaaaa
    24061 tgcaaagatc aacggcgtac ggacatctca ttaccccaac cagatcactt ggtattattt
    24121 atgcgaccaa gcaggcatcc atgtgatggc ggaaacgaac ctcgaatccc acggctcatg
    24181 gcaaaaaatg ggcgcatttg agccttctta caacgtgccc ggtgcagtac ctcagtggca
    24241 ggctgctgtt cttgatcggg caaaaacgaa tttcgaaaca ttaaaaaacc atccaagcat
    24301 tcttttttgg tctcttggaa atgaatccta tgcggaagaa aacattcgac aaatgaacga
    24361 ttacttcaaa aaagtagacc ccgatcgact cgtccattat gaagggaatt ttccaaatcc
    24421 agcattcgaa gattcgattt cagatgtaaa aagctgcatg tatgcgccac cagaagagat
    24481 cattgactac cttgaaaata aagcggaaaa accgttcatc ttatgtgaat acatgcatga
    24541 catggggaat tcgttaggtg gaatggattc ttacatgacg ttgcttgatc gttatgaaat
    24601 gtaccaagga ggctatatat gggattttaa agatcaggcg atccaagtga ctgatccaat
    24661 cactggtaga gaagtgatgc gctatggcgg tgactttgac gataaaccaa gtgactatga
    24721 attctcaggg aatggaatcg tttttgcaga tggacaagaa aaacccgcca tgcaggaggt
    24781 aagatattat tatgaaaaat acagtaaata aaagtcatat ggatacggaa aaagttgcaa
    24841 tcgtcttcgg cgactgtaca ttaggtgtca aatcggggaa tacgcattat attttttctt
    24901 atacaagagg cggactggaa tcgctcaata aaaacggaaa agaatggcta taccgggaaa
    24961 cacttccagt tttttggcga gcaacgaccg ataatgacag aggtagcgga tttggattta
    25021 aatccggtat gtggttagga gccggattgt atccaaaagt caaagaaata gaagtactgg
    25081 tggacaataa aaagatcgat ttgccgattg ctccagtaaa taatcaatat tcaagtaatg
    25141 aatacgctga aacggttgaa atcattttta cactggaaat caacacagta ccaaaaaccc
    25201 aagtgacaat ttcctaccaa atcgctggag agggtaaaat gcttgtcaat atgcattatt
    25261 atggacaaaa aggattaccg gaattacccg cattgggttt aaggttcatt atgccaacga
    25321 aagcaaaatt ttttgagtat gaaggcctat caggggaaac atatccggat cggatggctg
    25381 gtggtgtcga aggtatttat aaagtggcag gactccccgt ttccccatac cttgttcccc
    25441 aagaatgcgg catgcatatg gaaacgaaat gggtagaact aacaagaaat caaacgttgg
    25501 ataatacgga taaagatcaa gaagattttt cgttgcgatt tgaacagcag gatacgccat
    25561 ttgctttttc cgctttacca ttcaaagcag aagaactgga acaggcaaca catatagaag
    25621 aactaccgcc agaaagacga acggttctta cgatttttgc aaaagtaaga ggagtcgggg
    25681 gtatcaatag ctggggagcg gatgttgagt caccttaccg aatcagcagc gaagaggacc
    25741 atgaaatcgg ctttattatt cagtagcaat ctttagatta ttatttgaaa aacggaattt
    25801 acaagacaaa gctgctttat tctatatgtc atgaacaaga gagtaaaaaa gttctcagat
    25861 acttttgttc atgactattt tcttcaataa aaccaattgt tatttttctg aaaaacaaag
    25921 ttaactatca gaaaattaaa aaaagtttca taaaaatgat tgacagtcga tttttttatg
    25981 tctatactag gtacaattct tacatcagtt tggagcgatt ttttatgaaa tctagaaaac
    26041 ttacaaaact gaatacgtat tactttagct attttagata ggtttgtatt catgacttcg
    26101 tgggtacaaa caaaccaggt ttgtatctat gatggctaaa gatttttaaa cacgacttac
    26161 agccacgtag agaaattcat ctatagtggc tattttatat ttttgcagcg ctttttaagg
    26221 ctcgttgaag atgttcactg tagaggcaat ttcctacagg gacatctttt tttgttgatt
    26281 atctgggtca taagtaggaa agaaggagcg aaaatgagaa caacaggaca cggtcattta
    26341 gtaggaatta atgtaaaacc accttgaaaa agtagagaaa atcagccaag atagataagt
    26401 aaacaaaaga aagcgggaaa aacaatgata gtaattatga aaaaagaagc aacggaagaa
    26461 caaataaaaa aagtcgtcga tcatatcgaa aaagaaggat tcaaagtctc aatcgatcaa
    26521 ggaacagaac gagtagtgat tggactaaaa ggtgatacgc gaagtttaca agaaggagca
    26581 tttacacgct atgaaggagt agaaaatgct gtgcgtattt taaacacata caaattgact
    26641 tctcgagaat tccatcctgg caatacagtc gtggatgtag acggagtaaa aattggtgac
    26701 ggcagtttcg tgacgatggc tggtccttgt tctatcgaag gattagacca aatcagagaa
    26761 tgtgcgcgaa tggccaaagc cgggggcgca aaaattctgc gaggtggtgc cttcaaacca
    26821 cgtacttctc catatgcttt ccaaggttta gaagaagaag gattgaaata catccgtcaa
    26881 gcggctgatg aatttggaat gaaagtgatt actgaagtga tggatgaagg gcatatcgat
    26941 atggtggctg agtacagcga tatcctacag attggcgcac gtaatatgca aaattttaaa
    27001 ctgctatctg ctgtcggtaa aacaggtaaa cctgttggat tgaaacgtgg aatttccggt
    27061 acgatcaatg aatggcttaa tgctgcagaa tacattgcag tcgaagacaa atctcctgtc
    27121 atttttatcg aacgtgggat tcggacttac gaaacagcga cacgtaatac gtttgattta
    27181 agtgctgtcc cattgatgaa aaaattaact catttccctg tgattgttga tccaagtcat
    27241 ggtacaggaa tttgggaact tgtaccacca atggctcgtg cgggtgtggc aagcggagca
    27301 gatggcatga tcgtagagat ccaccctgat cctgcaaatg cttggtcaga cggtacccaa
    27361 tcgttgaatg aaaagactta ctcacgcatg atgaaagaag ttgcgatcat gaaagaagca
    27421 atggaaaaga ttcagagact cgattaattc cagcttttta aaagaaagaa gtgaaacgaa
    27481 tgcttactgt acatgttgat aaccaaaaat acgatattca tattgaaaaa ggcgcactag
    27541 aacaggctgg atcatggctt caaacgattt ggagacctca aaaagtagct gtgatcactg
    27601 atacaaacgt tgctccacta tatgcagaaa aaatcatgtt ccaattgaga caagctaatt
    27661 ttgaaccagt cctttcaatc gtaccggcgg gtgagaccag taaatcttta gaacaagcag
    27721 ctgagttata cgatttttta gcagataatg attttacccg cagcgatggg atacttgcac
    27781 ttggtggagg aatggtgggt gatctagccg cattcgtggc ctcgacgttc atgcgtggga
    27841 tccatttcgt tcaaattccg actacgctac ttgcacaagt agattctagt atcggcggga
    27901 agacagcagt caatacaaac aaagcgaaaa accttgtcgg aacatttagc cagccagctg
    27961 gtgtattgat tgatcctgag gttctaaaaa ctttgccggt acgtcgttta cgagaaggaa
    28021 tcgctgaaat tatcaaatca gcagctatag cagatgcaac gctttggaaa cagctgaacg
    28081 agctgaaaga cgaaaaagat cttctggaac atgctgttcc aatcattact gctacgttgg
    28141 aaatcaaacg aaaagtagtg gaagaagatg tctttgacca aggaaatcga cttattttaa
    28201 attttggtca tacaattggc catgcaattg aaaatacagc aggatatggg gtagtcagtc
    28261 atggagaagg agtagctatt ggaatggtag ccatcacttc tcatgcagaa aaaatcggct
    28321 tgtctccaac tggatcgaca gctgctttag aaaggatact tcaaaaattc catctgccga
    28381 ttacgtacga tctaagtcat ccagatgaat tattccacgc gataacccat gataagaaag
    28441 caagaggaag ccaattgaat atcatcttgc tagaaaagat tgggcaagca aaaatcgttc
    28501 ctgttcccac agagacattc aaagattact taggaggact gtaagatgcg ttatatgact
    28561 gcgggagaat cccatggacc agaattaaca gcgattattg aaggactacc agcaggatta
    28621 cctctgtcag aagaagaaat caatcacgaa ctcgctcgca gacaagctgg ttacggcaga
    28681 ggaggaagga tgaagatcga aaaagatcgt gttcggatca cgtctggcat ccgtcacggc
    28741 aaaacaattg gttcacctgt cactttagtg gtagaaaata aagattggaa aaattggaca
    28801 actgtcatgt ctgtagcaga agtgcctgaa aaagaccaat cactgcggcg agttgcacgt
    28861 ccgcgtccag gacacgcgga tttggtaggc ggtatgaaat accatcatcg agatcttcgg
    28921 aatgtgcttg aacggtcctc tgcaagagag acgaccatgc gtgcagcaat cggcgcaatt
    28981 gccaagaaaa ttctggaaga acttgacatc atcgtagcag gacacgtgat ttgtcttggt
    29041 ggaatccaag cagaaatacc tggaaatatc tcgattcaag aaatcaaaga aatttctgaa
    29101 gcgtcagaag tacgagttgt tgatccagca gttgagcaaa gaataaaaga tttgatcgat
    29161 cagacaaaga aaaatggaga tacgatagga ggaattgtag aagtgctcgt tggaggtgtt
    29221 ccagctggat taggaagcta cgtacaatgg gacacgaagc tagatgccaa aatcgcacaa
    29281 gctgtggtta gtatcaatgc ttttaaaggc gtagaatttg gggtcggatt cacttctggt
    29341 tcattaccag gctcacaagt gatggatgag atcctatggg aagaagaaag aggctatttc
    29401 cgacgtacca ataatctagg cggcttcgaa ggcggaatga ccaatggcga accacttgtc
    29461 attcgcgggg tcatgaaacc catccctaca ttgtacaagc cacttcaaag tattgatatc
    29521 gatacaaaag aaccttacaa agcaagtgta gaacgttcgg atagtacagc agttcctgca
    29581 gctagcgtcg ttgcggaaaa tgttgtagcc actgtggtag cgacagagct attgtcaaaa
    29641 ttcccaagtg attcgcttgt tgagttgaaa gttgcagtga aggagtaccg tgactacttg
    29701 aaacattatt aaaaaggggg caaataagat ggcacaacat gtttttatcg tggggcttgg
    29761 actgattggt gcttctatgg cactttgtat tcgtcagacc aatccagaac tgatagtagt
    29821 tggttgggat agtcagtcaa aaacccgtga gcaagcagta gaacagcaga tcgttgatta
    29881 cgttgcaagt gattttgaaa caggtgcaga acaagcagac gttatcttat tagccgttcc
    29941 cgtccgtaca accttagctt atcttgatgt ccttgaaaga accaacttgt ctgatcacgt
    30001 catcattacg gatgtcagca gtacgaagca gcaagtcgtg acatccgctg agcagaaaaa
    30061 tcttcggttt gtcggtggac atccaatggc tggttctcat aaatcaggta tacaggcagc
    30121 agatgctaaa ctatttgaaa atgcctatta catctttaca cctttggaaa agacgaaaaa
    30181 agatgttgaa aaactgcaag aactattttg cggaaccaaa gcaaaatatg taactcttac
    30241 agcaagagaa catgaccgta taacaggaat gctaagtcat tttccacata ttctggcagc
    30301 tggcttagtc aatcaggcag aacaattcaa tcaagagtat ccgcgagcca aacaactggc
    30361 agcaggaggc tttcgagata tcactaggat cgcttcctcg gatcctgtta tgtggacaga
    30421 tattttgttg agcaataagc agatattatt ggaacgattg gcggattggc agcaagaaat
    30481 ggcacaaata gcagaatgga tcatgactga gaaccaatcg gaaatctttt cttttttcaa
    30541 tcgggcaaaa gaaagtcgag accagttgcc tatccataag caaggcgcga ttcctgcctt
    30601 ttttgattta tttatcgatg tgccagatga acctggcgtg attgcagaag taactggact
    30661 gatcggaaaa gcgggagttt ctttgattaa tttgaagatc caagagacca gggaagatat
    30721 tttaggaata ctgcaaatca gttttaaaaa ccaacaggat ttgttacaag caaaaagaat
    30781 cattatgtct aataccagtt accattgctg gatcaaatag gaggctggat aaatgcaatt
    30841 attacaacaa atacatggat taagaggaac tgttaggata ccagcagaca aatcaatttc
    30901 tcatcgcagc atcatgtttg gggcaattgc ggaaggaacg acgactatac aaaatttttt
    30961 gcgcgcagaa gattgtctga gtactttaca tgccttccag caattaggcg ttgaaatcga
    31021 agaagaggaa gagatgatca agattcatgg tcgcggtagc cactcatttg tccaaccaac
    31081 cgaatcaatc gacatgggaa actccggtac gaccatacgt ttattgatgg gtattttggc
    31141 tggacagcct tttacgacta ctctgttcgg ggatgcttcg ttgtctaaac gtccaatggg
    31201 gcgagtgatg gaacctttac gcgagatggg cgctgacttg caaggaaaag agaatgagca
    31261 gtatctacca atcactgtga caggaaccaa ctctttatca cctatccgat acaatatgcc
    31321 tgtagccagt gcgcaagtca aatctgcttt gctgtttgcg gcattacaag cagaaggcac
    31381 atccgtaatc gttgaaaaag aacgttcccg taaccatacg gaagaaatga ttcgtcaatt
    31441 tggcggaagg atctcagtag aaggtaaaac aatcacggta acaggaccac aaaaattaac
    31501 tggtcaacag ataactgttc caggcgatat ttcatcggct gctttctttc tagcagcagg
    31561 gcttcttgtt ccggaaagcc aactgttatt aaaaaatgtc ggggtcaatc caacaagaac
    31621 cggtatctta gatgtgctgg aggagatggg cgcagcgatt acccatacga atcacaatga
    31681 acagaaccaa tcggctgatt taagtgtcaa gacgagtcat ttgaagaaag caacgattgg
    31741 tggagaaatc attccacgtc tgattgatga attgcccatt ctagctttag tggctactca
    31801 agcagaagga atcacgatca tcaaagacgc agaagaatta aaagtaaaag aaaccaacag
    31861 aattgatgcg gtggcagaag aattacagaa aatgggagca aagataaaag cgacagcaga
    31921 cggtctgatc atccatgggc cgacacctct gcagggcgca aaagtgtcca gcagaggcga
    31981 tcatcgaatc ggtatgatgc tgcaagtagc tgctttgata gctgatggac cttgcgaact
    32041 agaaggagct gaagctgttt caatctctta tccggcattt tttgacgatt tagcagaact
    32101 tgtgtcagga ggagaagctc atggctagca ttttgctgat tggtttcatg ggcgctggaa
    32161 aaacaacaat cggtaaaggg ttagctcaac gtttacaaaa agagtatgta gatttagata
    32221 caaagattga ggaacatatc caattgtcga ttgccgaata ttttcactat tacggtgaaa
    32281 aaagctttcg aaaagtggag tcagatattt taagaaagct gtcaaatgaa gataaaatta
    32341 tcgcgactgg tggagggatt gtccaaagtg cagaaaatcg gcgtttttta aaaacacagc
    32401 cgattgttct ttacttagag gcagaagcgg actgtctagt tgatcgtatc caacaagatg
    32461 aaacgtctat tcgaccttta gctcttggaa aaacaagaga agaaataaaa gcattattgg
    32521 cacaacgact ttcttggtat gaagaaagtg cgacacatcg aatcaacacg accaatcaaa
    32581 caccagaaaa gatcatcgat accattatag aaaggatcaa aagcatatga acgtcagcta
    32641 tctagggcca gaaagttcat tcacttatca ggcggcttgt cagctatttc ctacagaaca
    32701 attgactgcg tatgcttcca tccctgcttg tttgcgcgct ttgtttcgca aacaagtcga
    32761 tctggctgtc gttcctgtag agaattcatt ggaaggatct gttcatcaca caatcgatct
    32821 tctttccaaa catccagaag tcgaagttaa aagtgaaatc gttcttccta tcaagcagca
    32881 gcttttaggc aatccagcaa caaagattac aaaaatcctt tcacatccac aagcactagc
    32941 acaatcacaa cagtttttag aaacgcatta tcctaatgtt cctttagtag caacagaatc
    33001 aacaactgct gcagcaatgt atgtagcaga acatccgaaa gaagacgcag cagcgattgc
    33061 ttcattagaa acagcacagc atgttggatt ggagatctta gctgagaata tccaagataa
    33121 tgagttgaat cagacacgtt tttggatcat aggtgaccga aagatcacca gccagcagtc
    33181 agctccagta aaaatgagtg tcatcttgac actacctgct aatcggccag ggatgcttca
    33241 taaaatgctg gcagcttttg gctggcggga aatcaactta agtaagattg aatcccgacc
    33301 attgaaaact agcttgggag aatatttttt cgtgatcgat ttgttgttgg accgcccaat
    33361 gacattagta gagaatgcgc tccaagaaat caagatgctc ggaggagaaa gtcaaatatt
    33421 gggctgttat ccagtgctga cagtagaaga gacgcgtaga tgaaagtcaa gaatactgat
    33481 tgatttattt aacttaaaat aaaaaaatca gcgcttggct attctttatt tttgatattc
    33541 tgattgattt ttaggaagaa gcggtagaca aatcgttaca gtcgttcctg tcccaatcac
    33601 tgattggatc gttaagccat atgcatcgcc atacattagg cgtatccgct catctacatg
    33661 gctgatgccg attcctgaaa aatgatcatt tttttgtttg gtattttcag tatcgaagcc
    33721 atcgccgtta tcaatcactt cgaataaaag aagctgattt tttgttttgc cgtacaccgt
    33781 gatgaatcct tgcgttttat tgtgaaaagc atgaagatag gcgttttcga taattggttg
    33841 aaggatcata ttcggcatag ataatgactg cagttcatca ggaatcatta ttttagccga
    33901 gattctttcg ccatatcgca tactcaaaag atcgatgtaa ctttgtacgc ttttgatttc
    33961 ttccttgact ggaatcactt cttctttttt cccaatcgta cttcttagta aagtaatgaa
    34021 actgtcgatt ccttgaactg cttcatctgt ttttttctgc caaatcaaaa atttcaagct
    34081 agtcagcgta ttataaataa aatggggctg tatctgtgct tgcatagttt ggatttcaag
    34141 cgaacgtttc ttcttttcgt cttggatcgt ctgttcataa tgatgctgag cattacttag
    34201 caattgattg atcgtattcc ctagcaattg gacttcatgg cttccttgta caggaacagt
    34261 agcttttaag ccatcttcct ctagccgttt tagcgaatca atcaagcggt agatcgattt
    34321 tgtgtgtcga tccaaataca taaataccaa tgagccagca aaagcaagtg tcacgatatt
    34381 atacaaaata atcggaaaaa tcacgcccat ttggttagac aaagaatgaa gatcgacttg
    34441 actgagtaat gtacaatcct ggcggtacaa aggaagacgg gtcaactgct gtccatttga
    34501 aatcgttgtt tcacctactg ttagtttttt gaaaatcatt gatcggctcc caattgtatt
    34561 ttcttcattt gatgcgatga tcgtctgttc ttcgttcatg aaggagatct tcgatatctc
    34621 aggatttact acagattgaa aaagattcgc tagttgcttg gatgagatcg ataaaacagc
    34681 atatccgtaa acgtgattca atgtattttt cagttttttg acaaagaaga cgctgggtgc
    34741 tttcttatcg cgaagagtca atccgtggtg tgcaaaaagc atttgtgtat cagcagcacg
    34801 ttcattcact ctctgagcaa tcgcagattg aaaaaaagaa tcggcagata cggcagtgag
    34861 catgtcgttt ccgacaaaag ttcttccatt ttctccaaag atcaatacat cgtaatcgat
    34921 cattggatgg atttttgaaa gagagtcatt cattttttgg attgttctta gttgctgaag
    34981 ctgttccata tgttgggctg gatcattgga aaaatagctt tcgacagctg gattagaatc
    35041 aaaagcggct aatgtattct cgattgtatc attcaagctt tcaaattcag attggatctt
    35101 ttttagtacc acatcattcg attgattgaa cagctggata taggcatccc ttgatcgatg
    35161 ataaataaac aaagagatga cgatggcttg cagaaaaaga ccgatgaaca aaatgaacag
    35221 aagtttggta aagagttcat gctctttgat tttttttctc attttaaaaa agttccttta
    35281 cggtattgag aaggtgtcat tcctgtctct tctttaaaaa ctctggagaa ataacccagt
    35341 tcggtatagc cgactttctc acagatttcc gtcaaagtaa gccgtgtttc cgtcaaaaag
    35401 agcttggctt tactgatacg gacttttttt agatattttg aaaagttgat accaaatttt
    35461 tcagtaaaga tggctgagag atagctatat gaaaaatgat atttttcgga taaaagttgt
    35521 aaattgattt ctttctcata attttcctgg acgaactcgt agatcaaagt caagatatct
    35581 aaattgtctt tttttgcatt tgcagaaaag tgggtgagtt tctcaagtgt ttctttgaca
    35641 agaatagaaa aatcttcgaa atactcagca tcggctaata aagaaggaag ggcgatcttg
    35701 agcaaagaaa gttcttcatt cccaacatgc tcgtcagcca aagcgttgag taatgcatag
    35761 aaacgattga ttgcctcatg tttcaaataa gctggatcaa taggctgttc caatatcgtc
    35821 gtgaagaaat tctcaagttc tttgacagct aaagcgtagt ctttatgaac gatttttgtt
    35881 aaataactac ttgcttgctg ctcgtggttg gttaataacg gaagaagttc atgctgataa
    35941 gcaaggttct ttcccttatg atagaaacgt tgtcctttgg tgcaatttct gatctcttgg
    36001 aatatttcat tgatatcttg gattgcaaaa gaatcagaaa caacaaaaaa atcacctgga
    36061 aaaacttctt gacgaaactt ttgaacaaac ttcctaagtg tatctttgga atcagtattg
    36121 attacatagc cggattcttc cgaaaaggta gaaacaggat aaatagtaaa cgaatcatcg
    36181 ctatccatca atgaatgggt gatcgactat ttttgtttag agccttgttt tgaactgaat
    36241 ggccgttttt cggtatataa cagaaaaaac agggtgtgtt caaaaagaag agggtcaagc
    36301 aatggacgag tttgtccagc aatctgctgt gaaagattct cgcagatctg ctcataacga
    36361 ttggtgattg ggcttttgtt gggctttttt ttgatttttt gtgaaacggc agttaaggaa
    36421 tttagaaaaa aatcaggact caatcgaggc tttaaaagat aatcagcgac gccttcttta
    36481 aaagaatctt taaccatatc aaattcatca tagctagaga ggataaggat ctgtgtttca
    36541 ggttgttgct gttttaatag ccgcgataat tcgattccat ttaattcagg catcattaca
    36601 tctgtgacca cgatatcgat tgcatgtacc aaagaaaaat caagcgcttt caatgggctg
    36661 gaaaaagtcc ctattaattg aaacccgtgc tcagaccaat tgatcaactg tttcattcca
    36721 tctaaaatcg tctgttcatc atcgataaga attacgcgta ttggcatttt tttacccgcc
    36781 ttaagaatag tcttataatt taaaaatact tatttctagg agaaaatgca aggatatttt
    36841 tattttaaag aaacctcacc atttcgatct ttggtctaga tgctaagatt attccttttg
    36901 tcatagttga tttgtatttt tttattattt taggagaaat ttttgaagta tctgaataat
    36961 aaagaaaccg ttttcaaatt ttggtatgat gaatgtagca aacgtaaaca actaatggat
    37021 ggaaaaggag cggataaaaa tgaacatcag aaagttaatt gctggagtcg tttgtttagc
    37081 ggcagcatca ggtttcttaa caggatgcgg aagtgctagt agtacagcag acaacgaaga
    37141 aaaagtcact gtctgggcgt gggatgagac attcaatacc aaagcagtga atgaagcaaa
    37201 aaaagtttat gagaacgaag aaacagagat agaagttgtt acgatgtctc aggatgatat
    37261 cgttcagaaa ctgaacaccg ctttagcgag cgggaataca gaaggattgc ccaatatcgt
    37321 cttgattgaa gattatagga tacaaggcta tttgacttct tatccagatg cattcgctga
    37381 tttgtctgct attgtaaagg aagatcattt tgcaccgtat aaatttgcgg taaacaaagt
    37441 aggagataaa atatacggtg ttcctttcga ttcgggcgta acagcaaact tctatcgtac
    37501 ggatctgatg gcagaagcag gttatacaga agaggatatg gataatctaa aatgggacga
    37561 gtatattcaa gtagcgcgag atgtcaaaga aaagacggga aagaaaatcg ctgaagtcaa
    37621 tccttctgac cttggtcgag taaggatgat tatgcaacag gcaggagagt ggtatacatc
    37681 tgaagatgga gagacagtca cgatccaagt caatgcatct ttaaagtatg gtcttgaatt
    37741 gtttgccact attttgaaag aagatctagt cgaacaaacc tctgactgga atgctggagt
    37801 aactgcgata caatcaggag cagtagcttc tagcccaact ggcgcttggt attcttcaac
    37861 gatccaagga gcagaaaacc aatctggaaa atggaagatt gcaccaatac ctgcacttcc
    37921 agaaaatctg caaaaagcgc aagcttctaa ccttggcggc gctggctggt atgtgatcaa
    37981 aggagtagct ggagaagatt cagcgaaaga tttcctagaa gcaacgtttg cttcgaatga
    38041 agaactgatg ggcactcttg cgaaagaaat cggactagta tctacgatgc ttagcgcgaa
    38101 tgaacaagag gcttatcaag aagcttctga attttacagt ggtcaaaaga tctttgacga
    38161 tttttcaaca tggacatctg aaataccaga agtgaattac ggacatgaga cctatgcaat
    38221 tgaagcagtc gtagctgaag ctcttcatcg aatcatcaat ggagaagaga cagacaaggt
    38281 actagcagat acacaaaaac aagtagaagc acaattggct aattgatcgg tggtaaaaaa
    38341 gtgaatttct tcgctttttc atcagacaaa cgaaaagaca aaaaagatca cgtctgcctt
    38401 acagatgttg tctttcttgt cttttcatgt agataatcaa tgatagaatt ggagggaaaa
    38461 gaatgaagca gaaagattta ttcaaaaacg ggcatgcttt tttaatcttg ccagtcattt
    38521 tgatatcgat catggttttt attccgatgg ttttggcttt gatcacgtct tttcaaagtg
    38581 gtccccctgt caatatgaca tttaacggat taggtaatta tcagtggatg ctgacagatg
    38641 ctacttttaa aaaagctttt ttcaatacgt tcctgtattt actagttcaa gtaccaatca
    38701 tgctgtttct agcgcttttt atttgcaacc ttttaaatga taaaaagctg aaatataaag
    38761 ggttgtttcg aacagctatt tttcttccgt gtatcacttc gatggtcagt tattctctga
    38821 tcatgaaaag tctattttct caatctggcc ttgtaaacaa cttgttgttg ggcattggta
    38881 tgattgatac accgattggc tggctgactc atccaatttg ggcgaaagtt ctgatcattc
    38941 tttcgatcac ttggcgttgg actggataca atatgatttt tttcctttca ggcatgcaaa
    39001 atattgattc ggagatttat gaagcagcat ctattgatgg tgcttctaaa tggcagcaat
    39061 ttatcaaagt gacgatcccg aatttaaaac cgattatttt gtttacttcc attacttcga
    39121 cgatcgggac attgcggctt tttgatgaag tacaaaatat tactggcggg ggtccagcaa
    39181 atgcaacgac caccctgtct caatacatct ataatttgag ttataaattc acgcctaatt
    39241 ttggatatgc ggcagcagtt tcctttgtta ttgtttcctt tattgtgata ttgtcgataa
    39301 tccaaacgaa agtaggagga gaaagtcgat gaaaaatatg gtagaaaaag aaacaatcga
    39361 acgccccaac gtcaaaaaac aagcattaga acttcgttcg acaattgggt cagtattaaa
    39421 gtatacagca ttaagcatcc tatcatttat ttccattttc ccttttgtct ggatggtttt
    39481 gggaatgacg aatacacccg tggatatcac atcgggaaaa ataaagattg gcagtaactt
    39541 gatcatcaat tttcaaaatt tgttttccaa tgagctgaat tttactcgtt ccctttggaa
    39601 ttcagcgatg atcgctttgg tgaccactgt gtttgcattg ctgatttcgt caatggcagg
    39661 atatggattt gagatttatc aaagtaaacg aaaagaccta attttcaata ttctactgct
    39721 atcgatgatg gttccttttg cggcattgat gatccctttg tatcgaatgt tttcacaatt
    39781 gaacggaacg gttttgggaa tcaacagttt cttggttgtc atcctgccat ctgttagcac
    39841 tgcttttttg atcttcttct ttcgacaaaa tgttaaatct tttccaaaag atctagtaga
    39901 atccgctcga ttagacggtc taaaagaact aagtattttt ttagagtgta tatgccgacg
    39961 gctaaaaata cctatgccgc ggctgcaatc atcacattca tgagtagctg gaacaattat
    40021 ttatggccct tagtagcttt gcaatctcct gataaacgga cggttccgct tgttttatct
    40081 gctatgggtg cttcctatac accagactat ggaatgatca tggctggatt agtcatcgct
    40141 actttaccaa ctgccatcgt attttttgtt ttgcaaaagc aattcgttca aggaatgatc
    40201 ggctctgtaa aataaaaaaa acacaataag cgcgttcctt caaagtcatt tttccaagta
    40261 aaaagatact tttgggaagc gtgttatttt atgttgagaa aatttaaatg ataacgtttt
    40321 aagtttgtgc tcgaaaactc tttcatttta aggtaaactt aaagagtaaa ggaggaggat
    40381 acatgctaaa aaattttaaa gaagtgattg ctgctatatt gccgatgact gtcctgattg
    40441 tcattttaac gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg
    40501 gtgcggcaat catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga
    40561 tggaagtcgg agatctagta gggaaatata tgatcaaaaa gaaaagtttg actgttttgg
    40621 tcagtttggg atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt
    40681 tgggacaaca agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga
    40741 tcgtcagcgt cggcactgga gtatttttag cctttgcgct acttcgggtc gtattcaagc
    40801 tatcctatta tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca
    40861 ccagttccga atttatgccg attgcttttg attcgggcgg agtaactact ggtccagtaa
    40921 ctgttccgtt tattttggcc cttgccggag ggatgactag catgatcaaa caaaagaaaa
    40981 atgaaaatga cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga
    41041 tgattttggg ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat
    41101 cgttaccatg gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa
    41161 tatcattagt tttcgtatgc gcaaaaagcg tttctttgac attatgaagg gtttcttgat
    41221 aacgactatt gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg
    41281 acagcattta ggcagtgcta ttgcgggatt tccttacaat tatatactgg ttccccttgg
    41341 ctttttgatg gggttcctag tagggtttgc tgagccggcg attcatgtaa tggtcaaaca
    41401 ggttgaagaa ttgagtgaag gaaggatccg gtcgaaagtc atgctgatgg tgatttcgat
    41461 tgggattggc gcagcagtag ggttgtctat gtggcgtttg ctcgtcggat tctctcttta
    41521 ttatttcttg atcccaggat atgggctagc atttatttta ggacgaaaag tggacaagat
    41581 ctttttagca atggcttttg acaacggtgg agtagcaacg ggtccgatgt gttcgacatt
    41641 tattttatca atgagcgtgg cgatcgctgc acaaattgag ggaagagatc cgatcattga
    41701 cgggtttggt gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg
    41761 atatatttat aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt
    41821 gggggaaagc aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt
    41881 gacggaggaa tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga
    41941 acgatcttac atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac
    42001 atttcgccag aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta
    42061 atggatgcga ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt
    42121 attgatgtag acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga
    42181 atgcaagaac ttttagatga aaataaagat agagaaaagt ctgaatcata gacaagcgtt
    42241 tgaagaggaa ataaacataa acaagaaggc ggatgagcgt tatttccgct tttttgtttg
    42301 tatttttctc gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc
    42361 agactttctt ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa
    42421 ttttttagtt gttttaaaag atactctgat tgagcgatct acatttctat atgttcaatt
    42481 gcaggaaaat aaaacattgc tgcattcttc accagagttc catctagagg ggctaacatt
    42541 aggagaaatc tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt
    42601 ggaattacca gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt
    42661 tgaactgttt ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc
    42721 agcagaactg caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt
    42781 tagacgtcag caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct
    42841 gatcaaaacg acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt
    42901 tcctcaattg attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa
    42961 gctgtttctt ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt
    43021 gattgtgatt gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc
    43081 caattaaggc tttttctttt tggatttttt tgtcataata aatattagat gatggaggag
    43141 ataatatgat gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac
    43201 ttatgcaggg aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc
    43261 ttgtctttag tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg
    43321 tctttttcgg atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg
    43381 agaatcaacg ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataacaa
    43441 acaaaaagca agctaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc
    43501 atcaaaagca gcgtgcgcct aaaggaaaag gacgaatacg cgtgacatgc aaaacatgtg
    43561 gtataaaatt cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca
    43621 tggcgaaagc gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac
    43681 agaaaaagga aaagaacaag cagcagtagc cggtcaaaaa atgcttgaag ctggccttca
    43741 attcgatgcc gtttatgcct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact
    43801 agaagaaatg ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca
    43861 ttatggggat cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca
    43921 agtaaaaaaa tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt
    43981 tgaccgcaga tatgatcact tggacccgca tttaatccca tacggagaaa gtctggaaat
    44041 gacagtgaat cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg
    44101 gcaagattta ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga
    44161 agatgttcct gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta
    44221 tacattagca gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc
    44281 taaaagtgag agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta
    44341 tatatgggac tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg
    44401 gcaagcacta aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa
    44461 aaagtattct actagttatt tgatcaatga atcaaacttg aatgccaaag agtttaatga
    44521 attatttcac cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa
    44581 acaagtactg gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt
    44641 gacagaatca acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt
    44701 agtagggatc gatcaggact ttcctcgtaa accggatcca gcatctttaa attatttgat
    44761 cgatactttt catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat
    44821 cgaagcaggg aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg
    44881 tgagatcccg gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat
    44941 cattaaaaag tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat
    45001 aaaattatat ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga
    45061 gattgccgaa ccaagaagta gaaacagatt tgaaagtggg cttctcgcct ttaaaagaaa
    45121 cgatcgaaaa ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag
    45181 tgtttgatac atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga
    45241 aaattgaagg tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat
    45301 tcgatatcgt acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg
    45361 taaaattgaa ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga
    45421 ttaaagctat cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc
    45481 gataaaaatc gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga
    45541 agaatcattc tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag
    45601 gagaaaaatg acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata
    45661 tgtgttctat cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt
    45721 aggagttggt atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt
    45781 cgttactcaa tccatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca
    45841 aatgctagtt atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt
    45901 ttctgaaaag ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc
    45961 tattgcagca ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt
    46021 tacacaagga gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt
    46081 tgaaaactta tcgattccac agcagatttt atcctttatc cctaaaaacg tatttgctga
    46141 tttagcaggt agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg
    46201 aatcgcttat ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt
    46261 agtcgacagt ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc
    46321 ttatggagta tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt
    46381 caatttaggt aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca
    46441 ctcactgatt ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc
    46501 ggtattgagc tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga
    46561 gactcagaca aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg
    46621 tttgtctatt ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt
    46681 cgcaccagca gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat
    46741 tgtaacaatc agttcttttg gtgtagctgg agtcggtggg ggtgcaacat ttgcttcact
    46801 gatcgtttta ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgtcga
    46861 accattgatc gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat
    46921 cgtgacaagt gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt
    46981 gatcgatgtg aatgcatagt catagttaaa acatattagg aaatatacta agattatagg
    47041 gcaaaatcat cgatagatga ttttgcccta tatttttttg tgtgttttga aaaccacctg
    47101 ctatgcaggt ggttccggaa agcttctagc gaggtataaa aaaagccacc taaactgata
    47161 agatgaggtt tcccgaccac cacatcaaaa agtttaggag gtaccttttt atgaaaaagg
    47221 ctacagaaag tttatcacac actgtttgga aatgtaagta tcatatagta ttcgctccgg
    47281 aatttcgtca ccaaatcatt tatggcaatt ataaagcaag tattggagag attatacgcg
    47341 aattatgcga aagaaagggc gttgagattt tagaagcaaa tgcttgtcca gatcatatac
    47401 acatgttagt cagtatccca ccaaaaataa gtgtctctca atttttaggt tacctaaaag
    47461 gaaaaagtag tctaatgatt tttgatagac atgcagaact caaatatcgt tatggaaata
    47521 gaaagttttg gtgtagagga tattatgtgg acacagtagg acgaaacaag aaaaaaattg
    47581 cagaatatat tcgtaatcaa ttaagagaag accacgtagc aaatcagctt acattgtttg
    47641 aagaagttga tccgtttact ggagaaaaaa ccggaagaaa cactaagggc ttctttgaga
    47701 agcagcgagt aaatgtggag caagagggaa ccgattcggt aagcctttca ggcttaggcc
    47761 ggtaaaagag gctttcagtc gcagaacaaa ccacccgttc atacgggtgg ttttgattat
    47821 ttttggatta tagtgaggag tgaaagaaag ttcgaacaaa atcgtccttc ttatttacgt
    47881 atgttgagac tataagttac atgattttcg gcctaatcta atagttttgt tcaaccattt
    47941 aaacttaatt gcgtacttac ctatcctttt aagcttatca gtgaagaatc aaactctcta
    48001 tactagcttt tattttatca gataaatagc tcagaagatc atggtaggcg tataaataat
    48061 tcatgtagag aattcacttt ttatgctatc ctaaaactaa taaaaagatt atttggtctt
    48121 ac
//
LOCUS       DAPBBG010000019        47947 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPBBG010000019 DAPBBG010000000
VERSION     DAPBBG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.019"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            598..1287
                     /locus_tag="RD477_001210"
     CDS             598..1287
                     /locus_tag="RD477_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7633581.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="RD477_001211"
     CDS             1274..2482
                     /locus_tag="RD477_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7633582.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="RD477_001212"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="RD477_001212"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDT7633583.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="RD477_001213"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="RD477_001213"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDT7633584.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="RD477_001214"
     CDS             complement(6585..7280)
                     /locus_tag="RD477_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDT7633585.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="RD477_001215"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="RD477_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDT7633586.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="RD477_001216"
     CDS             complement(8479..8670)
                     /locus_tag="RD477_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDT7633587.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="RD477_001217"
     CDS             complement(8691..9581)
                     /locus_tag="RD477_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDT7633588.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="RD477_001218"
     CDS             complement(9568..10335)
                     /locus_tag="RD477_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7633589.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="RD477_001219"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="RD477_001219"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDT7633590.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="RD477_001220"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="RD477_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDT7633591.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="RD477_001221"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="RD477_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDT7633592.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="RD477_001222"
     CDS             14906..15289
                     /locus_tag="RD477_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633593.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="RD477_001223"
     CDS             complement(15457..16215)
                     /locus_tag="RD477_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDT7633594.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="RD477_001224"
     CDS             complement(16233..17051)
                     /locus_tag="RD477_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDT7633595.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="RD477_001225"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="RD477_001225"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDT7633596.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="RD477_001226"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="RD477_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDT7633597.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="RD477_001227"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="RD477_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDT7633598.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="RD477_001228"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="RD477_001228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDT7633599.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="RD477_001229"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="RD477_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDT7633600.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="RD477_001230"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="RD477_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDT7633601.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="RD477_001231"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="RD477_001231"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDT7633602.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="RD477_001232"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="RD477_001232"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDT7633603.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="RD477_001233"
     CDS             27093..27392
                     /locus_tag="RD477_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDT7633604.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="RD477_001234"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="RD477_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7633605.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /locus_tag="RD477_001235"
     CDS             27980..28216
                     /locus_tag="RD477_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDT7633606.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="RD477_001236"
     CDS             28359..30332
                     /locus_tag="RD477_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDT7633607.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="RD477_001237"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="RD477_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDT7633608.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="RD477_001238"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="RD477_001238"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDT7633609.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="RD477_001239"
     CDS             33211..34503
                     /locus_tag="RD477_001239"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDT7633610.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35239..36102
                     /locus_tag="RD477_001240"
     CDS             35239..36102
                     /locus_tag="RD477_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7633611.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36231..37853)
                     /locus_tag="RD477_001241"
     CDS             complement(36231..37853)
                     /locus_tag="RD477_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7633612.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38102..38623
                     /locus_tag="RD477_001242"
     CDS             38102..38623
                     /locus_tag="RD477_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDT7633613.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38809..40539)
                     /gene="spxB"
                     /locus_tag="RD477_001243"
     CDS             complement(38809..40539)
                     /gene="spxB"
                     /locus_tag="RD477_001243"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDT7633614.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40704..41522
                     /locus_tag="RD477_001244"
     CDS             40704..41522
                     /locus_tag="RD477_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDT7633615.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41625..41822)
                     /locus_tag="RD477_001245"
     CDS             complement(41625..41822)
                     /locus_tag="RD477_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633616.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41991..42569
                     /locus_tag="RD477_001246"
     CDS             41991..42569
                     /locus_tag="RD477_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDT7633617.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42664..42801
                     /locus_tag="RD477_001247"
     CDS             42664..42801
                     /locus_tag="RD477_001247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633618.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43614..44117)
                     /locus_tag="RD477_001248"
     CDS             complement(43614..44117)
                     /locus_tag="RD477_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7633619.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44476..45501
                     /locus_tag="RD477_001249"
     CDS             44476..45501
                     /locus_tag="RD477_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7633620.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45824..46702
                     /locus_tag="RD477_001250"
     CDS             45824..46702
                     /locus_tag="RD477_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDT7633621.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47178..47294
                     /locus_tag="RD477_001251"
     CDS             47178..47294
                     /locus_tag="RD477_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDT7633622.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT        14721 a         9024 c         9381 g        14821 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtat attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagaccag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg ttaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggcggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agactatgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg ggaaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatctgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaattcttga
    25441 aaactatatc gaacatcagc gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa
    25501 agcagaagca agggcacaca ttttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gcacagccaa aggaatacca attatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg ttagaggaat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaag atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaatagcca tcaagactaa ttgtgtaagc
    26881 tagcaagaat gaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taatcagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tggatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaacgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata tcgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    34621 gtgtcacagt ccctttttcc gaacaaacgg tgttcaaaag cttgcatcta cggcactaag
    34681 cccaatcaga tgaaaaatat ggagtgctat ttttatctga ttgttcttac tgcttgatgc
    34741 agagcagctt tttcacaacc tctccactaa acggtgttct cctgccaact cctcggtcac
    34801 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    34861 agctaaacgg caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta
    34921 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc cgtttttttc tctaatactc
    34981 aggtcaaaac gctgatttca caacctctga ttcacggtgt tccagaagta gcttctacgg
    35041 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    35101 ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc
    35161 ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac
    35221 gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac
    35281 catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa
    35341 gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa
    35401 aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt
    35461 tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc
    35521 tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag
    35581 atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct
    35641 gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat
    35701 tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt
    35761 aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg
    35821 gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc
    35881 cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat
    35941 gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag
    36001 ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc
    36061 tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg
    36121 taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat
    36181 cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat
    36241 aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca
    36301 gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata
    36361 aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc
    36421 aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta
    36481 gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca
    36541 ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag
    36601 aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga
    36661 tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc
    36721 caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt
    36781 gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat
    36841 atatcatcta aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag
    36901 tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt
    36961 tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat
    37021 tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct
    37081 tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa
    37141 tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat
    37201 actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc
    37261 aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc
    37321 aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca
    37381 ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt
    37441 aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat
    37501 tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt
    37561 tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag
    37621 tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta
    37681 acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct
    37741 gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca
    37801 tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt
    37861 cctcactttt atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa
    37921 aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa
    37981 aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt
    38041 tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa
    38101 aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact
    38161 tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt
    38221 tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga
    38281 gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg
    38341 cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt
    38401 atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag
    38461 tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt
    38521 cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt
    38581 tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata
    38641 aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat
    38701 ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga
    38761 agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt
    38821 ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg
    38881 gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg
    38941 cttgttattg atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa
    39001 ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc
    39061 aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc
    39121 ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag
    39181 ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact
    39241 taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc
    39301 attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa
    39361 taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc
    39421 agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc
    39481 ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca
    39541 gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc
    39601 accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat
    39661 ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac
    39721 aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc
    39781 agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc
    39841 gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt
    39901 tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag
    39961 caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg
    40021 ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt
    40081 atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat
    40141 gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa
    40201 agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg
    40261 gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc
    40321 gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac
    40381 ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat
    40441 aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag
    40501 aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa
    40561 tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta
    40621 attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga
    40681 tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc
    40741 aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt
    40801 ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg
    40861 tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa
    40921 atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat
    40981 atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat
    41041 tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc
    41101 ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag
    41161 ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag
    41221 gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt
    41281 acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa
    41341 cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg
    41401 tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg
    41461 cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat
    41521 aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat
    41581 ttttttagta agttatatca taatgaagtg aatggtatca tacattaata atagtttttt
    41641 ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga
    41701 actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag
    41761 ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc
    41821 atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta
    41881 aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc
    41941 tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa
    42001 atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag
    42061 aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt
    42121 ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat
    42181 ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg
    42241 cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc
    42301 cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa
    42361 aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt
    42421 acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag
    42481 tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa
    42541 gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa
    42601 tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg
    42661 cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt
    42721 tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta
    42781 ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa
    42841 agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat
    42901 ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa
    42961 gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga taggagatat
    43021 acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt gtggtttttg
    43081 gaataaaaaa ataacatatc tcattttacg cttttgttat tttatactta aaaaataacg
    43141 acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc
    43201 tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg
    43261 gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt
    43321 tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat
    43381 tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt
    43441 tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat
    43501 aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc
    43561 gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga
    43621 agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat
    43681 cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga
    43741 taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct
    43801 acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc
    43861 cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga
    43921 aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa
    43981 gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta
    44041 aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat
    44101 tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg
    44161 aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa
    44221 gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa
    44281 aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt tctaatttct
    44341 ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta
    44401 ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta
    44461 ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca agataatcct
    44521 tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat
    44581 catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa
    44641 aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt
    44701 gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct
    44761 ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc
    44821 ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta
    44881 ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt
    44941 acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga
    45001 tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt
    45061 tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt
    45121 gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg
    45181 attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag
    45241 ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga
    45301 aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt
    45361 cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt
    45421 ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt
    45481 gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat
    45541 ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat
    45601 tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat
    45661 tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt
    45721 cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt
    45781 atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat
    45841 gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt
    45901 aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga
    45961 ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa
    46021 gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc
    46081 agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt
    46141 ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta
    46201 tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg
    46261 tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg
    46321 aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag
    46381 agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca
    46441 agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga
    46501 gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga
    46561 tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag
    46621 tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg
    46681 cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca
    46741 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    46801 actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
    46861 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca
    46921 cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta acggacaaga
    46981 gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca
    47041 atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat
    47101 ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa
    47161 gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa
    47221 gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata
    47281 gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg
    47341 ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat
    47401 ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc
    47461 cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta
    47521 gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat cactttacct
    47581 tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata
    47641 atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg
    47701 caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag
    47761 ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa
    47821 aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact
    47881 attgctagaa taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt
    47941 gtgtatt
//
LOCUS       DAPBBG010000020        40017 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPBBG010000020 DAPBBG010000000
VERSION     DAPBBG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 40017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.020"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            194..450
                     /locus_tag="RD477_001252"
                     /pseudo
     CDS             194..450
                     /locus_tag="RD477_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            837..1064
                     /locus_tag="RD477_001253"
     CDS             837..1064
                     /locus_tag="RD477_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDT7633623.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1080..2246
                     /locus_tag="RD477_001254"
     CDS             1080..2246
                     /locus_tag="RD477_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDT7633624.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2481..3524
                     /gene="hrcA"
                     /locus_tag="RD477_001255"
     CDS             2481..3524
                     /gene="hrcA"
                     /locus_tag="RD477_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDT7633625.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3618..4181
                     /gene="grpE"
                     /locus_tag="RD477_001256"
     CDS             3618..4181
                     /gene="grpE"
                     /locus_tag="RD477_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDT7633626.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4232..6061
                     /gene="dnaK"
                     /locus_tag="RD477_001257"
     CDS             4232..6061
                     /gene="dnaK"
                     /locus_tag="RD477_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDT7633627.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6212..7378
                     /gene="dnaJ"
                     /locus_tag="RD477_001258"
     CDS             6212..7378
                     /gene="dnaJ"
                     /locus_tag="RD477_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDT7633628.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7746..8483)
                     /locus_tag="RD477_001259"
     CDS             complement(7746..8483)
                     /locus_tag="RD477_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7633629.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8485..9987)
                     /locus_tag="RD477_001260"
     CDS             complement(8485..9987)
                     /locus_tag="RD477_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDT7633630.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10246..12240
                     /gene="uvrB"
                     /locus_tag="RD477_001261"
     CDS             10246..12240
                     /gene="uvrB"
                     /locus_tag="RD477_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDT7633631.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12250..15066
                     /gene="uvrA"
                     /locus_tag="RD477_001262"
     CDS             12250..15066
                     /gene="uvrA"
                     /locus_tag="RD477_001262"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDT7633632.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            15329..16594
                     /locus_tag="RD477_001263"
     CDS             15329..16594
                     /locus_tag="RD477_001263"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDT7633633.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            16598..17329
                     /locus_tag="RD477_001264"
     CDS             16598..17329
                     /locus_tag="RD477_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDT7633634.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            17464..18348
                     /gene="rapZ"
                     /locus_tag="RD477_001265"
     CDS             17464..18348
                     /gene="rapZ"
                     /locus_tag="RD477_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDT7633635.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            18345..19343
                     /locus_tag="RD477_001266"
     CDS             18345..19343
                     /locus_tag="RD477_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDT7633636.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            19377..20312
                     /gene="whiA"
                     /locus_tag="RD477_001267"
     CDS             19377..20312
                     /gene="whiA"
                     /locus_tag="RD477_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDT7633637.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20880..21536
                     /locus_tag="RD477_001268"
     CDS             20880..21536
                     /locus_tag="RD477_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633638.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21715..22280
                     /locus_tag="RD477_001269"
                     /pseudo
     CDS             21715..22280
                     /locus_tag="RD477_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22979..23500)
                     /locus_tag="RD477_001270"
     CDS             complement(22979..23500)
                     /locus_tag="RD477_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDT7633639.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            23699..27379
                     /gene="nifJ"
                     /locus_tag="RD477_001271"
     CDS             23699..27379
                     /gene="nifJ"
                     /locus_tag="RD477_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDT7633640.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYVQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            27568..28455
                     /locus_tag="RD477_001272"
     CDS             27568..28455
                     /locus_tag="RD477_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDT7633641.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            28452..29621
                     /locus_tag="RD477_001273"
     CDS             28452..29621
                     /locus_tag="RD477_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633642.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            29645..31000
                     /locus_tag="RD477_001274"
     CDS             29645..31000
                     /locus_tag="RD477_001274"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDT7633643.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(31072..32877)
                     /gene="glmS"
                     /locus_tag="RD477_001275"
     CDS             complement(31072..32877)
                     /gene="glmS"
                     /locus_tag="RD477_001275"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDT7633644.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            33287..33577
                     /locus_tag="RD477_001276"
     CDS             33287..33577
                     /locus_tag="RD477_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633645.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33590..34615
                     /gene="sppA"
                     /locus_tag="RD477_001277"
     CDS             33590..34615
                     /gene="sppA"
                     /locus_tag="RD477_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDT7633646.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            34618..35277
                     /locus_tag="RD477_001278"
     CDS             34618..35277
                     /locus_tag="RD477_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDT7633647.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            35434..36366
                     /locus_tag="RD477_001279"
     CDS             35434..36366
                     /locus_tag="RD477_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633648.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            36520..37809
                     /locus_tag="RD477_001280"
     CDS             36520..37809
                     /locus_tag="RD477_001280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDT7633649.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            38056..39306
                     /gene="clpX"
                     /locus_tag="RD477_001281"
     CDS             38056..39306
                     /gene="clpX"
                     /locus_tag="RD477_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDT7633650.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            39389..39976
                     /locus_tag="RD477_001282"
     CDS             39389..39976
                     /locus_tag="RD477_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDT7633651.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT        13190 a         6911 c         8751 g        11165 t
ORIGIN      
        1 aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa aaagtagttg
       61 gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg atcatttact
      121 acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt tctaaaatca
      181 catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga ttacggttcg
      241 tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg ggattacttt
      301 atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca atcgtcaaac
      361 ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat tcatggaaga
      421 atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg cccgaaattc
      481 agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa tcaattcgtt
      541 gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac tgtttatttt
      601 tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat ttgactagat
      661 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
      721 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
      781 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
      841 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
      901 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
      961 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
     1021 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
     1081 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
     1141 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
     1201 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
     1261 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
     1321 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
     1381 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
     1441 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
     1501 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
     1561 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
     1621 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     1681 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     1741 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     1801 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     1861 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     1921 atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg
     1981 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     2041 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     2101 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     2161 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     2221 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     2281 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     2341 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     2401 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     2461 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     2521 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     2581 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     2641 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     2701 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     2761 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     2821 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     2881 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     2941 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     3001 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     3061 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     3121 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     3181 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     3241 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     3301 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     3361 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     3421 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     3481 aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     3541 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     3601 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     3661 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     3721 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat
     3781 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     3841 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     3901 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     3961 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     4021 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     4081 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     4141 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     4201 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     4261 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     4321 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     4381 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     4441 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     4501 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     4561 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     4621 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     4681 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     4741 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     4801 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     4861 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     4921 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     4981 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     5041 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     5101 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     5161 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     5221 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     5281 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     5341 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     5401 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     5461 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     5521 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     5581 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     5641 cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg tgaaagatgc
     5701 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     5761 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     5821 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     5881 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     5941 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     6001 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     6061 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     6121 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     6181 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     6241 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     6301 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     6361 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     6421 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     6481 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     6541 aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     6601 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     6661 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     6721 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     6781 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     6841 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     6901 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     6961 aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa gtgatatctt
     7021 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     7081 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     7141 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     7201 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     7261 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     7321 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     7381 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     7441 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg
     7501 tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa
     7561 ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg
     7621 gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta
     7681 agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcgaccggg catttttggt
     7741 ttttattaaa tatttaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt
     7801 tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca
     7861 cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct
     7921 tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca
     7981 gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc
     8041 acacgctgct tttgtccacc ggaaagcatt tcaggataca tatctttttt atcttctaga
     8101 ccaactgtct gcagcaattt gattgctttt ttctctgctt cttctctaga ttctttcttc
     8161 aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag
     8221 tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc
     8281 aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa
     8341 cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg
     8401 ttgatggaca cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc
     8461 tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt
     8521 tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact
     8581 ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc
     8641 ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat
     8701 gatgcgtatc gcttgcggca aaatgatttt ttgcattgtc cgattataag aaagacctaa
     8761 acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc
     8821 tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat
     8881 cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg
     8941 aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc
     9001 tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt
     9061 taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc
     9121 aagaaatgtt gattcatcag cagtggaaga ctcactggaa gtttctgaac catccgctac
     9181 atattcttta ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt
     9241 gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc
     9301 agattctttt ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac
     9361 cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc
     9421 agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc
     9481 agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt
     9541 gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga
     9601 aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc
     9661 ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt
     9721 aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc
     9781 ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc
     9841 ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt
     9901 gtcagcttgt gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa
     9961 tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat attttattat
    10021 tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg
    10081 agtttctaac attttatgtt ataaaacgta acatgcataa taagataaaa aagtggcgaa
    10141 agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt
    10201 attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat
    10261 acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg
    10321 attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg
    10381 actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca
    10441 ctaattattg cccataacaa aacattggct ggtcagctgt acggggaatt caaggaattt
    10501 ttccctaaca atgctgttga atattttgtt tcttattatg attattatca gcctgaagct
    10561 tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac
    10621 aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca
    10681 tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg
    10741 attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa
    10801 ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg
    10861 gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag
    10921 gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac
    10981 gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta
    11041 gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt
    11101 ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg
    11161 ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa
    11221 ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg
    11281 cacgtaacca tgcctcaagt cagaggaatg tataatggtg accgagcgag aaaacagatg
    11341 ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa
    11401 gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa
    11461 catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca
    11521 gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa
    11581 cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac
    11641 ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa
    11701 acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt
    11761 ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg
    11821 gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga
    11881 gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg
    11941 cgtctagcga tggatgaaac atcaagacgc cgtacgattc agcagaagta caatgaagaa
    12001 catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa
    12061 gaatcggaag atgatacaaa agaagcggta caagtctcgt atgaagaaat gacgaaagaa
    12121 gaaaaagata cgttattgat gaaattagaa aaagaaatga aaaatgcggc gaaagcattg
    12181 gattttgaaa cagccgctaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag
    12241 gaggacaaca tggctaacga taaaattacg attcacggtg cacgtgccca taacttgaaa
    12301 aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct
    12361 ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag
    12421 agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc
    12481 atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg
    12541 tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta
    12601 ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg
    12661 gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg
    12721 gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc
    12781 cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac
    12841 aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct
    12901 cgtctatttg attctttaga ggcggctctg cgattggcag acggctatgc tctggttgac
    12961 gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt
    13021 actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca
    13081 gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga
    13141 acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat
    13201 cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa
    13261 gaattaccgg aagagcatca ggatatcatc ttgaatggga cagatgatct tttccatttc
    13321 cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg
    13381 aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt
    13441 ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct
    13501 ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa
    13561 gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg
    13621 attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta
    13681 actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct
    13741 acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga
    13801 ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc
    13861 aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatctgatt
    13921 gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa
    13981 gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc
    14041 cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca
    14101 gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca
    14161 ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg
    14221 caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa
    14281 agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat
    14341 ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag
    14401 gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt
    14461 gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat
    14521 gttccttgtg aagtatgtca tggcaaacga tataactctg aaacattaga agtgcattac
    14581 aaaggaaaaa gcattgctga tattttggaa atgacagtag aagatgctgt agaattcttc
    14641 aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat
    14701 gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt
    14761 gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg
    14821 acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat
    14881 gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat
    14941 atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca
    15001 ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg
    15061 gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt
    15121 gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt
    15181 atagacaaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag
    15241 tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa
    15301 acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat
    15361 ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc
    15421 atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa
    15481 aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtgttta tcgagacgat
    15541 gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg
    15601 tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat
    15661 ctgtccatat attgattatt ctttatttga aagactgatc aataaagtaa gcttttatga
    15721 agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt
    15781 agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc
    15841 aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga
    15901 gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga
    15961 aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta
    16021 tgtagatgaa gatcatcaag ttcggatgat gtgtcttggt catccactgt tagaagatcc
    16081 tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta
    16141 tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat
    16201 gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg
    16261 tagcagtttc tttgtgacat tgaacggctt gaatctggct cgtttcgtca cagaagatcg
    16321 agtgtttaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag
    16381 attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa
    16441 aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtgt tttatgaaaa
    16501 agaccggtca atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag
    16561 attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt
    16621 aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa
    16681 agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga
    16741 tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga
    16801 tattgagaaa caaaatttat tgcagccgaa cttcattgtg ttgacttgta acaccgcaca
    16861 ctactttttt gaagaattac aagcggcaac ggatattcct atccttcata tgccaagaga
    16921 agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga
    16981 aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggat tcgaaacagt
    17041 gattcctgat actgttcttc aagaaaaaat caattatctg atctatcatg agatcaagga
    17101 atcggattat ttgaatcaag agctatatta cgaaatctta gaagaagcgg tagagcgcct
    17161 aaactgtgaa aaagttatct taggttgcac agaattgtca ttgatgcatg aatttgctga
    17221 agataaccat tatccagtga tcgatgccca atctatttta gcagatcgga cgattgaacg
    17281 agcgctagct gaaagaagca aggcattgga tacagcatcg gaaaagtaaa aaaattggat
    17341 ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac
    17401 tggtaatgat acgcccggaa tgatataatt ttactatgca caatgtgaga ggagttcgta
    17461 attatgacag ataatttgca attagtcatc ataacaggta tgagcggtgc agggaaaaca
    17521 gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga
    17581 ttgattccaa aattctggga attaatcaaa gaatcaggca aagtcacaaa aatggcttta
    17641 ggagtggatt tacgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc
    17701 gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta
    17761 gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact
    17821 gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc
    17881 attgatacga cgacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa
    17941 tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg
    18001 ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg
    18061 gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat
    18121 gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac
    18181 attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt
    18241 tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg
    18301 acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct
    18361 accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc
    18421 tgaaaagttt gcgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg
    18481 ggggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa
    18541 atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt
    18601 tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag
    18661 tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg
    18721 tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtctttaaag
    18781 acggaacgac tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc attgaccgtg
    18841 tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag
    18901 caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc
    18961 caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct
    19021 atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata
    19081 tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg
    19141 aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg
    19201 cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttgg
    19261 aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt
    19321 tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt
    19381 catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga
    19441 aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt
    19501 ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga
    19561 aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga
    19621 acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata
    19681 tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa
    19741 aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa
    19801 cgagccgtta tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg
    19861 atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt
    19921 cttatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact
    19981 caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc
    20041 tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg
    20101 aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg
    20161 aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga
    20221 ttccctcagg tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat
    20281 ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca
    20341 ggagatgcga atgtatcagt tttttttaaa agttatactt gcttttcttc cgtcaaacta
    20401 taattccatc tttactggag taacataatt tggaaaggct tctattgttt ttaatacaat
    20461 cgtataaaaa gcggggagac aactgaatga agattttagt tttgtacata aatttttgct
    20521 taatatagaa gtcctgatat agataagatc aagtatttat ctataactgg cctgattaat
    20581 taaaaaactg gaaggaactg ctcgatcatc cttgagaact gctcttctta cttctggtca
    20641 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    20701 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    20761 taatcataag ttcataaata gtaattgaga tgtatggttg gaatacgtta tacctgaact
    20821 tgaactacca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    20881 tgcgtttttt tacaactttt actatcatta tgattgcagt actttttatt tttctagata
    20941 tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca ggtctcataa
    21001 catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt tggagaactc
    21061 cagcaccacc attgccagag attacatatg gagaatttcc ttttagactt gagtatgaat
    21121 tgaatgaaga attacatgtg atagaagaca cgttaatagt tgaatttgat ggatttggaa
    21181 tgaacgaagg catagggaga tatcgtagat ggacaagtcg tttagccagc ggagaagatt
    21241 tagtgttgct gttagaagtt agtgataata agcaaatatt ttattttcca ggacctgcta
    21301 attattacat gggagatagg ttgaatggtt ataatcatac ttttcctagt gcttctttca
    21361 ttgaaaggga aagaggcatt actagaaggg atattttaca tgacaaagaa ttattagaac
    21421 aatttggtcc gcttgatcaa aatacaataa atgaagaaga attgttaaat caatataata
    21481 taaggctagt gaattgggaa atatctgaac cgattgttaa caattttggt gattagaaaa
    21541 caattacgat aaatagaaac aagccaaatc atcagcaagt aaaagctgat gatttggcct
    21601 ttcttatacg agtagtagga attcatagcg aatattatta cgatcagtag atattttgat
    21661 attaaaggag tattacgaac tagtatgtga gaatgtagag gtgatttgcg aataatgaga
    21721 aatttatcaa atagaaacaa aatattaatc attattgttg ttatagctgt gtttcatctg
    21781 ggaacgaatg cggttttaag tagaataata ttgggtccta agccaccaag acctgcgatt
    21841 acccgagggg agtttgattt tagattagaa tatgaagtag atggggaaag gattgtgata
    21901 gaagatacaa ttgttgcatt atttgatgga ttttctgcag atgcaggtag catggcatgg
    21961 tatcgcacgt ggcgtttgca tttggcgagt gacaagaggg cacaacaatt attgttacat
    22021 gagttagatg atggtcgaaa aatatattat cattttggag gagctggatt ttatatgggg
    22081 aagttagtag cagtgtaact gtaacaagga aagatttgcc aggtcttggc attcgaaact
    22141 ccatatttta tatcgagcgt gggagagata catctattgg gaacaggaaa gggttaactc
    22201 ttgaagagtt atataatgac tttggaatcc gtcttgttag ttgggaacac aatccaccaa
    22261 tagaaaatcg ctttgaataa tcgaaatcta ttaatccatt tcatcagcca gtgaatgttg
    22321 taatggaaca atttggttta gtgttcaaaa ccattgaaat actaggctgt gatcagcaaa
    22381 aatgtatctg aataaaaaag agagcaacta ttagctgaga ggagttaata gttgttagct
    22441 ttatacgaat caaagtggca aatacagatg cgtatgcatt tgtgtttgcc atcttgcgtt
    22501 tggtagacga gataggtata agctaaagaa acaacttgtt taaaataaac gacctagctc
    22561 aacaaatgac catctaatca actaacagca tacataaaag caactatttt tgaagtagaa
    22621 ttgaaatcgg gataacttaa aaagtgattg ggatactagt gagaatcacc gtacataaat
    22681 attactcgtt aagggaaatt gagaaatttt gtatgaaaat ttaagaatta gttagtatgc
    22741 gcaagtcaaa aatatgaata aattatttgg tggagataat ttgcttagct tgtcataaga
    22801 aatgagataa tctggcagat gcaagctttt ggacaccatt tattcggaat aaggaggctg
    22861 ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc gccgtttata
    22921 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctcc ttattttttt
    22981 attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca aagatttctt
    23041 tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg ttgatgacag
    23101 cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact gcaaaatccg
    23161 ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt tgtttgatct
    23221 ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg ttccctcgct
    23281 gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc ccgctttgga
    23341 ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac ggacaagcta
    23401 agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg attccttttt
    23461 gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac ctctttcgct
    23521 tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt ctaaaaatta
    23581 gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa aaaaatggca
    23641 tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat gatgaatcat
    23701 gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt atgcatttac
    23761 cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat tagtagatca
    23821 atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag tagaaatgca
    23881 atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg cactgacaac
    23941 tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca agattgctgg
    24001 ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa atgcgttgaa
    24061 tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg ctatgttagc
    24121 tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg cttctattga
    24181 aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg agattcaaaa
    24241 aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga aattagcttc
    24301 atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga accagaatcc
    24361 agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat tacctgaaat
    24421 cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg acttggtgac
    24481 ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag ttgcacagac
    24541 gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt ttttgaatat
    24601 ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc atacagtaaa
    24661 aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac ctctcttatt
    24721 agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg gtggtcgcta
    24781 cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg atgaattgaa
    24841 aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg atgtgactta
    24901 tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat tccaggccaa
    24961 gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta tcaagattat
    25021 tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga aaaaatctgg
    25081 cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag cgtatcttgt
    25141 cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct atgacttggt
    25201 caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg acgaacaatt
    25261 agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca ttagattcta
    25321 tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga tcaatacggc
    25381 aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg tgcttccgct
    25441 gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg tagaaaaaaa
    25501 catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc cagaccattg
    25561 gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttatg tacaagaaat
    25621 acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac ttgtagcaaa
    25681 tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc ggggcgtcgc
    25741 tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat gtgcctttgt
    25801 ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg aagaagcgcc
    25861 agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc gtatccaagt
    25921 ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg caaaagaaaa
    25981 agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt gggcttttgc
    26041 aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct tgtctacaca
    26101 attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg aaacacctta
    26161 tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg cgacaggctg
    26221 ttcttctatt tggggcggtg cttctcctgc ttctccttat acgacaaatg aatgtggtca
    26281 agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt atggtatgct
    26341 tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag tattgccaaa
    26401 agtatcagaa gaactacgag aattgatgga agactggttg gtacacatgt ttgagagcga
    26461 aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac gatcaattgt
    26521 ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca gccaatggat
    26581 gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc atgtacttgc
    26641 cagcggagca gacgtgaata ttcttgtttt ggataacgaa gtctattcca atactggcgg
    26701 tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta gtggaaagta
    26761 tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt atgtggcgca
    26821 aattgcttca ggtgccaatc aaatgcagac gataaaggct tttgaagaag cagaaaagta
    26881 tcctggacct tctttaatca ttgcctatac gccttgtatc acacatggtt tgcttggtgg
    26941 aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggctatt ggtcattata
    27001 tcgatataac cctgcaatgg tcgaaaaaaa caagaaccca atgactttgg actacaaaaa
    27061 acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt cctcgctaga
    27121 gaacgcaaat ccaaatcttg caaaagcttt atttgaaaag actatccaag acgctaaaac
    27181 acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga aacgacttga
    27241 accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga cggaccttga
    27301 agcagaagaa agaagagctg ccagagcaga acgacgtaaa aaaatggaac aagctacaaa
    27361 agaaaaaaag gaaaaataac agaaaaaata taatgaataa ttctctgaag tttaaaaata
    27421 tttattgcag agaaaaataa aacgcatact tgcaattttc ctgaaaatta tgcgtttttc
    27481 tcaagaaaaa tacgagcaga tcagcctcta aaattaaaac atatgatata ctagagagga
    27541 aatctcaaaa gagaagagag gctttttatg tcatttcaaa tcggggaact ttttaatttg
    27601 gactattggc agcaaatgtt ttccggtgac ttgttctctc tgaatttttt ggtgaacata
    27661 cttgatattc tggttgtctg gtatcttgta tacaaactga tccaactgct gcgaggcaca
    27721 aaagcgattc agcttctgaa aggtgtagct atttttattg ttatccgaat cgtagcggaa
    27781 atcattggtc tgcacacctt gtcttggctg atgaaccaag tcattacgta cggggtgata
    27841 gctgctatcg tgatttttca gccggaaatc cggagaggac tggaacatct tggacgcagt
    27901 tcgcttttca aacaatcaaa aagcgaacag caggaagatg agaagatgat cttgtctttt
    27961 gacaaagcca tccaatatat gtccaagcga aaaatcggtg cgctaattac tatcgaacgg
    28021 catactggat tagatgagta tattgagaca ggaattgcat tagatgcaga tatcactgga
    28081 gagctgctga tcaatatctt tattcctaat acaccgctgc atgacggtgc agttattgta
    28141 aaagaaggaa agatcgcagt agcaagcgcc tatcttcctc tatcagaaag tatgttgatt
    28201 ccaaaagaat tcgggactcg acaccgggca gctgtcggta taagcgaagt cagcgacgcg
    28261 attaccatcg ttgtctctga agaaactggg gatgtcagca ttacattaga taatgaactg
    28321 atggctggct tatcccagca agaatatttg gcaattttgc gtagagaact gattgctgaa
    28381 gagaaaacaa aaaacaagaa aaacgtgatt caatctttcc tagacggtat gactaaggga
    28441 ggggatcgca aatgatctca aaagagaaac ggacaagcat tctttacagt tttattgcat
    28501 tgctgttcag tattgcgctg tacttcaatg caaatggcca gtccgttcca aatacgcttt
    28561 ctggaaatga agcgtataat caaacagtta ctggggtgcc aatcaagatt tcttatgatt
    28621 caaagcgcta ctatatccat ggatatgaaa atgcagtgac tgtcaaactt tccagtgcaa
    28681 accgagttca gttgaatgca gaggcaaacg aagatacacg gatgttccgc gtcacagcag
    28741 atatcactaa gctgggcgaa ggaacacatg aggttccgct aaaaattcaa aatttgagca
    28801 gtgctgtcac agcaaagatc gaaccatcga ctattacagt aacggttgag aaaaaggtaa
    28861 taaaagattt cgatgtggaa acgttgattc cttcaacgtt cacaccatct ggatatgaac
    28921 ttgatgatac ttccgttagt cctaaaaaag tctctatcac tacaggtgac aaaacattgg
    28981 cagaaatcaa acgagtagta gctgatgtgg acgcttcgat ggtcacagat gatgggatta
    29041 atagtgaggt cccaatacag gcgctgaatg cagcagggga agtattaagt atcgtctctg
    29101 atccagtgca agtaacagtg agagctgatt cgatcaagcc aagcaagtcg gtacgacttt
    29161 atgggataca gcaaggaaca ccagctgccg gggtagaaag ctatgatttc agtttttcaa
    29221 aactggaagc agaagtaagc ggctctagcg atttgcttgc atcgatcggt gacagtattg
    29281 ctgtaccgat caatgtctca ggtatcaatc atcgaacaac tcgtaaaatt gaaataccag
    29341 tagaagaggg gttaagtatc aagcccgaat cagttagtgt ggagattaca cccgtgttgc
    29401 aaacgtcaag cagtagctct gcttcgacga gcagtcggac acaagaaaat acaagtacga
    29461 gtagaagtat ttcatctgct gattcaggaa cggaaccatc gacaacaacc atttcttcca
    29521 gttcggaatc tgggacaagc agctcgacca caagtacaga agaaacatca gaaacaagcg
    29581 aatcaacaca aatgagcaga acaaattcct cagaaaatta agttaaagga gtaattttac
    29641 aactatgggt aaatattttg gaacagacgg cgtacgcggt gaagccaaca aggaacttac
    29701 gccagaactt gcatttaagc tgggacggtg cggaggttat gtcctaagcc agcatgaaga
    29761 agaaggaaaa cgcccacgcg tgttagtagg gcgggataca agaatctcag gtcaaatgtt
    29821 agaagaagcg ctgatttcag gacttttatc ggtgggtatc gaagtgttcc agctaggtgt
    29881 gatctctaca ccaggtgttg cttatctgac acgtttgcaa aaagcaagcg caggggtcat
    29941 gatttctgct tctcataatc cagcacaaga taatggaatc aaatttttcg gtgcagatgg
    30001 ttttaaacta gtagatgacc aagagctaga aattgaagca ttattagatg ctgaagtgga
    30061 tgaattgcct cgtccatcag ctgaaggatt gggaactgta gaagaatttc cagaaggctt
    30121 attgaaatat tctcaattcc ttgtacaaac gatcaacggt gacctttccg gtttgaccat
    30181 ttgtgtcgat gcagcaaacg gagcaacttc aacaagtgtt aatcgtttgt ttgccgatct
    30241 tgaaacagat ttttatacca tgggcacaaa tccagacggg ttaaatatca atgatggcgt
    30301 aggctctacc catccagaac gtttagctga gatggtcgtt gaaaaaggag cagatgctgg
    30361 actagctttc gatggcgatg gcgatcgtat tattgcggtg gatgaacttg gaaacattgt
    30421 cgacggtgat aaaatcatgt ttatctgtgc taaatacttg gcagaaaaaa atcgattgaa
    30481 aaaagatacg atcgttacga ctgtcatgag taacttagga ttccacaaag cggtggaagg
    30541 aatcggtttg aaagatgtcg ttacacaagt tggtgatcgt tacgttgtag aagaaatgcg
    30601 caaaaacgat tataactttg gcggcgagca gtctgggcac atgattttct tagattttaa
    30661 cacaactgga gatgggatgc tttcaggaat tcagttactg aatatcatga aacagacagg
    30721 taagaaatta tcagagcttg cagcagaggt aacaatctat ccgcaaaaat tagtgaatat
    30781 ccgagtaagt gataaaaacg gtgcgatgga tgttccggca atcaaaaaag tgattgaaga
    30841 ggcagaagct gaaatgggat cagaaggaag aatcctagtt cgtccttccg gtacagaacc
    30901 attacttcga gtgatggcag aagcgcctac tgatgagaaa gttaattact atgtcgataa
    30961 aatcgcggca gtagtcaaag aagaaatcgg actggattaa gagatatttt cataaaaaat
    31021 aaaagaacaa ggaccagtgt cagatgtctg acactggtcc ttgttctttt attattctac
    31081 tgtcactgat ttcgctaaat tacgtggttt gtcaacgtcg aatccccgta atagcgttgc
    31141 gtaatatgca agtaattgag tcggtacgac tgtggttaga gtagttagat aagggtggac
    31201 agttggtagc accagctggt cactttcctt ttctaatccc tctgagacaa taactagtgt
    31261 tttagcgcca cggctttcga cttctttcag gtttcctcga gtatgcgcac cagtcacttc
    31321 ttcactgatg attccaatca ctggtgttcc ttcttcgatc aaagcaatcg ttccatgttt
    31381 caattcgcca gcggcaaaac cttctgcctg gatataactg atttctttca atttcaaagc
    31441 agcttcaaga gaaacgttgt agtcagcgcc acgtccaatg taaaaggcat ttcttgtatc
    31501 gctgagatat acttctgcca actcttctaa ataatctttt tgggcgatga ccgattccat
    31561 cgcattagca actaaactca attcatgagt gatatcgaat acaagagaag tttcaaattc
    31621 ttttttatca ccgatagctt tggctaataa agttagtaca gctatttgtg cagtataagc
    31681 tttcgttgat gctactgcga tttcgggtcc agcatgcaat agcagtgtaa aggatgcttc
    31741 ccgcgaaaga gtagaaccag caacatttgt aatcgtaagt gaagggtagc cgagacggtt
    31801 gatttttacc agaacttggc ggctatctgc tgtttcgcca ctttgactca agaaaataaa
    31861 gaatggtttt tttgaaagaa gaggtgtgtt atagccaaat tcactggcta aatgaacctc
    31921 aacagggatc tgtgtcaatt tttccagagt ctgtttacct gctaaccccg catggtaact
    31981 agttccgcaa gcaacgatat agattcgatc gctatcagac aatgtatcga ccagcttttg
    32041 atcaagagag acacgattgt cttctgttac gtatttttga gcgatacgac gcatgataat
    32101 aggctgttcg tcgatttctt tcagcatata aaaaggatag gtacctttct cgatatctga
    32161 agaatctact tgtgcttcga atgaagaacg ttctacgatg tcacctgcaa aattttgaat
    32221 aataactttt tctgatgtga cggtaaccat ctcttcatct tcaatctcaa cgaattcctt
    32281 cgtatgagca agcatagcca ttgcatcgct ggcaatcaca ttgaatccat cgccgagtcc
    32341 gattaatagt ggacttttat ttttagcgac gtaaataaca tcaggtgctg tccgatcaat
    32401 caaagcgaaa gcgtaagaac ctttgatgac ccgcaaagct tttaaaaacg cctcttgcgt
    32461 tgtagtatcc ttcgcgaaag tttcaattag atgagcgata atctcagtat cggtctcacc
    32521 aataaagtgg tcatttgcta aataagcaat tttaagttct tcaaagtttt cgattacgcc
    32581 attgtgaacg agcacgaagc gtccactttg ggaagtatgt ggatgggcgt tttcttcaga
    32641 aggttgcccg tgagttgccc agcgtgtatg accaatacct atatttcctg tagtatcaat
    32701 atttattttg tttttcaaat tttgaattct gccttgtgct ttgaccagat aatcagttgt
    32761 gccatctgag acaaagatac cagctgaatc ataaccacgg tattctaatt tttctagacc
    32821 gtcaatcaat cctggtgtta cattttttaa tccaatcatt ccaacaattc cgcacataaa
    32881 aaacatccta ctttctataa tgaattcgta tgaatgcttc cagctgcagg aaaatccttt
    32941 tgttttatct ttgcagatat tttttgtgga ttttctgtag agtgcagtcc ctagggccat
    33001 ccgccgaatc tttcgataaa ccctctcctc gtcatctgtt tacacagacc tggcgctatc
    33061 ctaaaaggta taactatcct cctcttttgt tttttatctg gaaacattta aaatcataac
    33121 cttttgcgaa tgttcttgtc aacaaaaaaa gaataaaaaa taaattggta tatatcaaat
    33181 gctttgtcaa atgctctttt tatgtcaaaa aataatgggt ttttatctat catctttttc
    33241 aggagttcgg tacaatagaa agtaatcgga tagaggagga gtaaaaatgg attcaaaaat
    33301 tgagatcatg acactgggaa tgttaaaaaa acaattgtcc aaattcgaag catcagctgg
    33361 tgtatcagat gatacgaaaa tatttttaga tactggctgg gatagcatcc aagaaatcgc
    33421 acctgatgcc ttggaagttg ctgaagctcg tgagtttacg gtagaagatg aattgacaaa
    33481 agagtcgttt tccgggtatg cgcgggaaga aaaagcagag cggttcgaca cttctgagca
    33541 gtcagaaact gtgatcgtca taaaaaactt gtattagaaa ggagaaagaa tgaataagag
    33601 acgttgggca gcagttctga ttgctgcagg attatttgtg gtttcgttag tttcagctag
    33661 tttgactaag cctcaggaaa acgatcaaat tgagggattg aacagttggt tatatgggga
    33721 tgaagaacta gcttcaaatg tgttggaaga gggagacagt acgcaaaaaa tcgtaaaatt
    33781 atcagtagat ggaacgattg ctgcgggagg gtcttcaggt ttatttgctt ctgaaggtta
    33841 caatcataaa aattttatgg aacaactgaa ggcgattgaa gaagatccaa cagtcaaagg
    33901 tatcttttta gaagtaaatt ctcctggtgg cggtgtctac gaaagtgcgg aaatcgctaa
    33961 gaaattggat acgattcgta aagaacatga tatcccaatg tatgtcagca tgaaaaacat
    34021 ggcagctagc ggtggctact atatttctgc tcaagcagat aaaatctttg caacagaaga
    34081 aacagtgacc ggctcaattg gtgtgatcat gtcaggattg aattattcag gacttctaga
    34141 aaaactgggt gtggaagata caacagttaa aagcggagcg ttgaaagata tgggttcagg
    34201 aacgcgacca gagacaaaag aagaagaagc ggtgcttcaa gcatatatcg acagcgcata
    34261 tagtcgtttt gtagcaatcg tcagcgaggg acggaataaa tctgaagaag aagtcaaaaa
    34321 aattgctgat ggaaggattt atgatggtgt ccaagcaaaa gaagtaggtt tagtcgatga
    34381 attgggattt cctgaagatg ctttaaaagc tatgcgaaag gaacaacagc ttgaagatgc
    34441 ggaactagta gaatattctt ccacgccatc tgggtttgct agtacatggt taggaagtaa
    34501 actagcagaa ttccagggat tgaagccttc tgagactagt cagatcttat ccgttcttga
    34561 gaatctaggg acagcggaat caccaaaagc aatgtattat tacggaggtg agtaagcatg
    34621 tcagagcttt caaatgaaaa taaggaacaa gaaaaagata agcaacagca gcctgctgta
    34681 ggacctgact ccaacttttc ccaaaaggta ctgagctctt ttgaaagaga gcctttgacg
    34741 cccgaagaaa tcaaaaagaa acaacgtaaa tggaaaaagt atacggacac aagaaatgaa
    34801 ccgccaaaag caaatgattt tcctaagtat ttctatgcag gtttttttat ccgaatgttt
    34861 gctttcttgg tcgatctttt gtgtatcgga gctattacgc gtttaacact tggtattgcc
    34921 aaaaatctag gttggcttaa tttttcagat tcttatctga gtatctacgg attcacagcc
    34981 ttacttattt atctagcgta ttttatttta ttgacgaaat tcaatcatgg tcaaacgatt
    35041 gggaaaatga tctttggtat tcgagtcatc agctttgatg aagaagaact tagctggtca
    35101 accgttttag ttagagaagg tgcttgccgg ttcattctaa aatttccttt gctgatgtta
    35161 gggtatcttc cagcaatctt taatcagaaa aagcaacatc taggcgattt tttcactaat
    35221 acaagtgttg ttacgatcaa tctgatcaaa gcattcaacc aggaagtcaa cagctgatga
    35281 gcaataaaaa gcagtaacag ttgaaagcac cttctaaaag tcagatctaa aatctagctt
    35341 ttagagggtg cttcagttat ttttaattgt catgcttttt ttgttttgct actattgaac
    35401 atgagctgaa catgagcaag aggaggacac tgcgtgaagc cgattgattt tccagaaaaa
    35461 tatgagaatt taatgcgagt agcacaacaa gcactagcaa accagcaata tcagcaggca
    35521 aaagaattat ttcaacgagc ttacgagttg aaagaaagct ttgaggctaa tagcctgctt
    35581 gttttttgtc tttacgagtt ggacgagaaa aaagaagcat tgaagcaagc actcttgcat
    35641 gaaaaacagt atcttcaaaa tgaggagttt gctgaatttt attttgactt gttaatcagt
    35701 acacaagatt ttttatatgc tagaaaattg atagcaagta cagattttta tgaagctttc
    35761 gagcagcggg tcattgaaaa aatccaattt gcagaagaat tatccggaca aatggagagg
    35821 cagaagatca aggcactcca caataaatcg gaggaacttc cttcgttgga acctgcacgt
    35881 cagcttgcgt ctattgatga gatcgaacag cttccttatc atgagtttat ccaaacagcg
    35941 agtaaactta ttgtatcgcc agaggtccat attttagcaa gggcaaaatt actggagaca
    36001 cttaggcaat tgaacgaatg ccatcctgtt ttctatttga cgattgaaga aaagttagtc
    36061 aaagtcatac caaaagatct gccaaagccg cagcagcagg caagttacca gcaattatgt
    36121 gtattttcag atcgttacgg taatgaagat gcgctgctta gttccgtgtt aaatgaagaa
    36181 ttcacgttgc aatctgcaat tgtttatcct gtctatgata catacatcga agacccgaag
    36241 cgctggtttc aactgactgt agaggcgtgt acaggaaaaa cgatgtatga aggtacagaa
    36301 gatgaaaaac aagatttttt aaaaaaaaga gaaaaaattt tgcagcaaat gatcttcttc
    36361 cattagattt tatcagtgaa aaagataaaa atgttatgaa aaacaaggat tctatccatt
    36421 caatatgttt acaatttgaa agggttagtg tactatttaa aggtatgcat gaaacgtttt
    36481 tgatgtataa aatgaatttc taaactcgga gggaattaaa tgactgcaac atttgaaaaa
    36541 aaaggcacga atgatggtgt tttaaaattt acgattgcgc aagacgaaat ccaaaaagga
    36601 ttaacaactg cgtttaataa agtgaaaaaa agcttgaacg tacctggatt ccgtaaaggg
    36661 aaagtttctc gtcaagtatt caaccgtatg tacggtgaag aagctttata tgaagatgca
    36721 ttgaatgctg tattaccaga agcttatgaa gctgctgtca aagaagctgg catcgatcct
    36781 gtttctcaac caaaaatcga tgttgaaagc atggaaaaag gtcaagactg ggtaatcact
    36841 gctgaagtaa cagtgaaacc agaagtaaaa ttaggacaat ataaagatct agttgttgaa
    36901 aaacaagacc gtgaagtaac agatgctgac gtggatgcac gtatcaaacg tgaacaagaa
    36961 gcacaagctg aattagttat caaagaagat gctgcagctg aaaatggcga tacagtagta
    37021 atcgacttcg aaggctttgt agatggcgaa gcgtttgatg gcggaaaagg cgaaaattac
    37081 tcacttgaat taggttcagg ctcattcatt ccaggttttg aagatcaatt agttggtcat
    37141 aaatcaggtg aaagtctaga tgtaaatgta actttcccag aagattatca agcagaagat
    37201 cttgctggaa aagaagcagt cttcaaagtg acaatccatg aagtaaaagc aaaagaagtg
    37261 cctgaattag atgacgaatt cgcaaaagat gttgatgacg aagtatcaac attagacgaa
    37321 ttgaaagaaa aatatcgcaa agaattagta gaaacaaaag aaaaagcagc ggacgatgca
    37381 aaagatgaag cagcgatccg tatggctgtt gaaaacgcag aaatcgttga attgccacac
    37441 gtgatggttc atgatgaagt acatcgttca atggacgaat tcttgaacaa catgcaacgt
    37501 caaggtatct ctcctgaaat gtactatcaa ttaactggtt ctacagaaga agacttgcat
    37561 aaacaattcg aaggtgaagc agaaatgcgc acaagaacaa atcttgtgat cgaagcaatt
    37621 gcagctgctg aaaatcttca agcaacagaa gaagaaatca atgcagaaat ccaagaatta
    37681 tcagaaacat acaacatgcc aatcgaacaa atcaaacgcg tgttgacaga agatatgctg
    37741 gaacatgatg tgacaatgaa aaaagcaatc gaattgatca caggtacagc gacagaagcg
    37801 aaagaataga aatcaagtaa taaatcattt ctgaagaagc tacttctaga aaattgttta
    37861 ttcgaaataa gggactgtga cacgattttg tcacagttcc ttattttttg ttaagagtaa
    37921 aaaatgcaaa ggtataggtc agttgatcct ttgttaaaca ctttttcttt ttactaaact
    37981 atgttatgat agtaaaagac tgtaaagtct ttgatagttt cttgcatact ttttgcaagt
    38041 taaggggtga agaccatgta tgacaatcca agtagcaatg agaccgtacg ctgttctttt
    38101 tgcgggaaaa cccaagaaga agtgaaaaag atcgtagctg gtccaggggt ctacatctgt
    38161 aatgaatgta tcgacctatg taaagaaatc attgatgaag aattttatga agaagctgta
    38221 cgcgaattca ctgaagtccc taaaccacta gaaattcttg aagtattgaa taactacgtg
    38281 attggacaag atcgcgcaaa acgctcgtta gctgttgccg tatacaatca ctataaacgt
    38341 gtcaatcagc aagcacaaga aaacacagat gatgttgaac tgcaaaaaag caatatctgc
    38401 ttgatcggac ctacagggtc tggtaaaaca tttttagcac aaacattagc aaaaacattg
    38461 aatgtacctt ttgcaatcgc agatgcgaca agtttgacag aagctggtta tgtgggggaa
    38521 gatgtagaaa acattttgct taaattgctt caatctgctg attacaatgt ggaacgtgca
    38581 gaaaaaggga tcatctatat tgatgagatc gataaaattg cgcgtaaaag cgaaaatgta
    38641 tctattactc gtgacgtttc cggcgaaggt gtccaacaag ccttgctgaa aattctagaa
    38701 ggaactgttg caagcgtgcc acctcaggga ggacgtaaac atccacatca agaatttatt
    38761 cagattgata cgacgaacat attgttcatc gtaggtggtg catttgatgg catcgaaacg
    38821 attgtgaaga atcgtatggg cgaaaagacg attggatttg gtacacaaaa ccaaaaactt
    38881 tctgaagacg aaagtgtcat gcagcatatc attcctgaag atttactaaa attcggattg
    38941 atccctgagt ttatcggacg attacctgtt atggcagctt tagagaaatt gacaacagat
    39001 gatctggtac ggatcttgac tgagccgaaa aatgcgctag tcaaacaata tcaaaaactt
    39061 ctttctctag ataataccga acttgaattc gaacctgaag cgttgagagc cattgcgaaa
    39121 aaagcaatcg aacgcaatac cggtgctcga ggacttcgtt ccatcattga agaaatcatg
    39181 atggatgtga tgtttgatat cccttcaaat gaaaacatcg aaaaagtgat tatcacaaaa
    39241 gaagcagctg aattgtctgg tgagccaaca gtaatctata aagattcaga gaaaaaagcg
    39301 ggttgatgaa aagaggctgc cgtaaaagtc attttggctt ttgcgacagc cgcttgcttt
    39361 attcatcacc ttgttaagga gagagaccat gaaagtccat caagctgaaa ttatcataag
    39421 tgcagtggca ccaaaacaat atccagatac acaattgcca gaaatagctc tggcaggacg
    39481 atcaaatgta gggaaatctt cttttatcaa cacattgatt gatcgaaaga atttagcccg
    39541 tacttccggt aagccaggaa agacccagac attgaatttt tatttgatcg agaacgcatt
    39601 acattttgtc gatgttccgg gatatgggta tgcaaaagta tccaaaactg agcgagcaaa
    39661 atggggaaag atgatcgaga catatttaac gactagagac cagctacgtg cggtcgtttc
    39721 tttagtagat ttgcgtcata agccttctca agaagatatc cagatgtatc aatttttgaa
    39781 gtattatgat atccctgtaa ttgttgttgc cactaaggca gataagatcc ctcgtggtaa
    39841 atggaacaag catgagagcg aaatcaaaaa agcgttggat tttgatcgca atgacacgtt
    39901 tattctcttt tcttccgtta caaaacaggg aaaagaagaa gcgtgggcag caatcgaaca
    39961 agcaattgaa tcataaaaag aataaaaaag acttaccagt caaactcttt cataaaa
//
LOCUS       DAPBBG010000021        39056 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPBBG010000021 DAPBBG010000000
VERSION     DAPBBG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 39056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.021"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(117..1253)
                     /locus_tag="RD477_001283"
     CDS             complement(117..1253)
                     /locus_tag="RD477_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7633652.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(1369..2277)
                     /locus_tag="RD477_001284"
     CDS             complement(1369..2277)
                     /locus_tag="RD477_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDT7633653.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDFYK
                     PDGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSAQG
                     TAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(2368..2796)
                     /locus_tag="RD477_001285"
     CDS             complement(2368..2796)
                     /locus_tag="RD477_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDT7633654.1"
                     /translation="MEMDTINLVEELKRKYQSANPFCICEKMGIKVQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(2801..3175)
                     /locus_tag="RD477_001286"
     CDS             complement(2801..3175)
                     /locus_tag="RD477_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7633655.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            3460..3645
                     /locus_tag="RD477_001287"
     CDS             3460..3645
                     /locus_tag="RD477_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7633656.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKNILDIR"
     gene            3658..3906
                     /locus_tag="RD477_001288"
     CDS             3658..3906
                     /locus_tag="RD477_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633657.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKKTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3937..4116
                     /locus_tag="RD477_001289"
     CDS             3937..4116
                     /locus_tag="RD477_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633658.1"
                     /translation="MGKFNRALVFSTPFIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(4159..4629)
                     /locus_tag="RD477_001290"
     CDS             complement(4159..4629)
                     /locus_tag="RD477_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633659.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            4716..5414
                     /locus_tag="RD477_001291"
     CDS             4716..5414
                     /locus_tag="RD477_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDT7633660.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDKNE"
     gene            5593..5933
                     /locus_tag="RD477_001292"
                     /pseudo
     CDS             5593..5933
                     /locus_tag="RD477_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5926..6660
                     /locus_tag="RD477_001293"
     CDS             5926..6660
                     /locus_tag="RD477_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDT7633661.1"
                     /translation="MSEGTKRNDNKLFNSLYKITVNDVVEKRNKLTYLSWAWAWAEVS
                     KICEEVDYEIYRDPETHRPYLFDEKTGYMVFTSITVNGVKRDMWLPVMDGANKAMKDE
                     PYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLGLYIF
                     AGEDMPEDVSMLEPASQRSKKLFLDALQLVANKYDKSIDEAIVALTDAASITADDSKW
                     TKRDLGILKRGVNWLEDQYREETKEK"
     gene            6666..7352
                     /locus_tag="RD477_001294"
     CDS             6666..7352
                     /locus_tag="RD477_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633662.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIRATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLNIGG"
     gene            7355..8104
                     /locus_tag="RD477_001295"
     CDS             7355..8104
                     /locus_tag="RD477_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633663.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRDLPPSKKSKAKPIRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            8116..8385
                     /locus_tag="RD477_001296"
     CDS             8116..8385
                     /locus_tag="RD477_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633664.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYEQLEEAGIEGIKNNRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCIKGLQKHLSELEQV"
     gene            8547..8858
                     /locus_tag="RD477_001297"
     CDS             8547..8858
                     /locus_tag="RD477_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633665.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKKWT
                     N"
     gene            8846..9157
                     /locus_tag="RD477_001298"
     CDS             8846..9157
                     /locus_tag="RD477_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDT7633666.1"
                     /translation="MDELITKVEQWAKDKGLNQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            9181..9399
                     /locus_tag="RD477_001299"
     CDS             9181..9399
                     /locus_tag="RD477_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633667.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            9396..9767
                     /locus_tag="RD477_001300"
     CDS             9396..9767
                     /locus_tag="RD477_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633668.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGHRNTGESYD
                     VATAHEVIGNIYENSELLEVE"
     gene            9764..10102
                     /locus_tag="RD477_001301"
     CDS             9764..10102
                     /locus_tag="RD477_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633669.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            10099..10329
                     /locus_tag="RD477_001302"
     CDS             10099..10329
                     /locus_tag="RD477_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633670.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLSDGTEIVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            10336..10539
                     /locus_tag="RD477_001303"
     CDS             10336..10539
                     /locus_tag="RD477_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDT7633671.1"
                     /translation="MNTRHRRVAKLRKQELNVLKAKFEKEYGVSVEEAYKVVSQCVAD
                     ASETIRKFGISILNDDRKWEEMR"
     gene            10536..10841
                     /locus_tag="RD477_001304"
     CDS             10536..10841
                     /locus_tag="RD477_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633672.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKIKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            10825..11118
                     /locus_tag="RD477_001305"
     CDS             10825..11118
                     /locus_tag="RD477_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633673.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEQQK"
     gene            11378..11791
                     /locus_tag="RD477_001306"
     CDS             11378..11791
                     /locus_tag="RD477_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDT7633674.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            complement(12162..12515)
                     /locus_tag="RD477_001307"
     CDS             complement(12162..12515)
                     /locus_tag="RD477_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633675.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            13062..13274
                     /locus_tag="RD477_001308"
     CDS             13062..13274
                     /locus_tag="RD477_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633676.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQSKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            13281..13508
                     /locus_tag="RD477_001309"
     CDS             13281..13508
                     /locus_tag="RD477_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633677.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            13505..13789
                     /locus_tag="RD477_001310"
     CDS             13505..13789
                     /locus_tag="RD477_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633678.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            13830..14402
                     /locus_tag="RD477_001311"
     CDS             13830..14402
                     /locus_tag="RD477_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633679.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            14403..14714
                     /locus_tag="RD477_001312"
     CDS             14403..14714
                     /locus_tag="RD477_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDT7633680.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            14705..14965
                     /locus_tag="RD477_001313"
     CDS             14705..14965
                     /locus_tag="RD477_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633681.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            15133..15687
                     /locus_tag="RD477_001314"
     CDS             15133..15687
                     /locus_tag="RD477_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7633682.1"
                     /translation="MIGLNSKLVGTRMKQKRIEKGLTQADVATKSGISSRYYGSIENG
                     KNSPSIETLNSISIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVE
                     GLIIQAARLRVLLDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAI
                     VKQLTDLLPQGTRADKKSKLLGRK"
     gene            15690..17381
                     /locus_tag="RD477_001315"
     CDS             15690..17381
                     /locus_tag="RD477_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDT7633683.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            17413..18279
                     /locus_tag="RD477_001316"
     CDS             17413..18279
                     /locus_tag="RD477_001316"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633684.1"
                     /translation="MRIKGKNISWVFYMLGILAFTFVAIKLSEIIIPNDSVVSKNLVL
                     ERAKLIISILGPALTLIVFVRTIQTQLESSKNLIKDNVSKDFYNLLNLFKSIQDKMNS
                     ELFSTIEKRIKVPINNINTNLDVRENKETTKEVQEDLKEYSASLNKVLSESVKKINEI
                     FEDNYKDIGSYLKVFHRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILIN
                     SLFTLRGTGMGIELIETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKT
                     RKKIENYLKIER"
     gene            18408..19580
                     /locus_tag="RD477_001317"
     CDS             18408..19580
                     /locus_tag="RD477_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7633685.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            19567..20271
                     /locus_tag="RD477_001318"
     CDS             19567..20271
                     /locus_tag="RD477_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDT7633686.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            20276..21436
                     /locus_tag="RD477_001319"
     CDS             20276..21436
                     /locus_tag="RD477_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDT7633687.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            21512..21790
                     /locus_tag="RD477_001320"
     CDS             21512..21790
                     /locus_tag="RD477_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDT7633688.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            21771..22142
                     /locus_tag="RD477_001321"
     CDS             21771..22142
                     /locus_tag="RD477_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDT7633689.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            22158..22562
                     /locus_tag="RD477_001322"
     CDS             22158..22562
                     /locus_tag="RD477_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633690.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            22559..22969
                     /locus_tag="RD477_001323"
     CDS             22559..22969
                     /locus_tag="RD477_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633691.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            23030..23617
                     /locus_tag="RD477_001324"
     CDS             23030..23617
                     /locus_tag="RD477_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7633692.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            23690..24157
                     /locus_tag="RD477_001325"
     CDS             23690..24157
                     /locus_tag="RD477_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDT7633693.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            24203..24511
                     /locus_tag="RD477_001326"
     CDS             24203..24511
                     /locus_tag="RD477_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633694.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            24706..31470
                     /locus_tag="RD477_001327"
     CDS             24706..31470
                     /locus_tag="RD477_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDT7633695.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLNLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLTAGTTNAKDTKLSDETKVADPIIPDSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKVANTMLQSKLSEFFTHGNFDGE
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDDDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            31474..32295
                     /locus_tag="RD477_001328"
     CDS             31474..32295
                     /locus_tag="RD477_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDT7633696.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGNREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFNIPSGSAESIATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            32304..33347
                     /locus_tag="RD477_001329"
     CDS             32304..33347
                     /locus_tag="RD477_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7633697.1"
                     /translation="MTDLIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSD
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYDT
                     VTGARSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQRTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTEDPKDSDYRFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPSI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            33347..34720
                     /locus_tag="RD477_001330"
     CDS             33347..34720
                     /locus_tag="RD477_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633698.1"
                     /translation="MGLLKLISNRISTEWKEKFNQNIDYLNDLEKKLSDQDKSTNSRI
                     DNLVLHSGGDSPNEVVDARINAEGTIYPTLYSRLLALDNLFNLNYTELKTRQDNQQGQ
                     LNQLNVSVGTLMGAYGETLDLYVAKTGSDQSGDGTEKNPFLTIQAAVNQIPLLTSSRV
                     TIWIGDGVYLEDVVIRNLKAVSITLRSRQSVTDVTSDLSVKVRSISFISSLGYQQVNG
                     IEFVDQANISGQLKCAIYSEQSSYLAVWNCRFAETTYGKSNRCLFATGGSKIATNNNY
                     YLNQNCIAEARNLADINIDLSDQGTGNDYGIIADNGTARIKVVGSKVKANRIAEVRNQ
                     GNVVTGKIIRQITNDDISDRDNITNVNGTIKREGDTVTIAIKYECNNYPSDASNTRNV
                     ILVPAGFQRDQSYPAYHPLALYRNETQPAGARAGLTQASRVVAYSGNGSSYISGTWVT
                     NDPIPII"
     gene            34736..36916
                     /locus_tag="RD477_001331"
     CDS             34736..36916
                     /locus_tag="RD477_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDT7633699.1"
                     /translation="MFKINESIIVIQAEAIRPNNTGVVFWSHDRGTAKLRMKLVRKNG
                     IPQSLPEGTTVPIRLMFKSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGIV
                     EGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILADG
                     KQEIEQKITKSETQIDAKLKDTNDKITKANQDVATINTNIDKANDRIGQTNQQISDLG
                     KLKKMYSNSIDFGNYDYSGNPNLLSKLSYDLIENQNTSAGTLSKGENSFKYNKISAET
                     EGGVELYYKRRGIANWLPSNKTLVMTVKLRAGVDYSPADGKKILIRYRFVDNGTGKIV
                     LDLPINSNSITQEWKEFSITGTTPTFGPQAYHPWIQFRAQDGILGEIEMSYDIKIEEG
                     PTATPYQPNLLVEPYNMCREYPNENIADPKVKFPIESGDHQIYQGYTEEELMIGQTYT
                     ITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQYP
                     RSTVGACQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKALTN
                     TLNLTDPQTALGIKNFKDGARIDGVDVATVKNLGSVLQSGSGLTTIQSDKQSAFDEFS
                     YEFERIGDRVFFHARVKTNSTTAVASHVNLLEIPLGFQFPVEWTIVGIPLSVVQWTTP
                     QGEISALVITDLTGKQVVKFATNRVGNHYISGEWRTADSYPETK"
     gene            36928..37374
                     /locus_tag="RD477_001332"
     CDS             36928..37374
                     /locus_tag="RD477_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633700.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGFRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            37376..37513
                     /locus_tag="RD477_001333"
     CDS             37376..37513
                     /locus_tag="RD477_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDT7633701.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            37548..37790
                     /locus_tag="RD477_001334"
     CDS             37548..37790
                     /locus_tag="RD477_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDT7633702.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            37806..38003
                     /locus_tag="RD477_001335"
     CDS             37806..38003
                     /locus_tag="RD477_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDT7633703.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            38014..39033
                     /locus_tag="RD477_001336"
     CDS             38014..39033
                     /locus_tag="RD477_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDT7633704.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT        13786 a         6137 c         8145 g        10988 t
ORIGIN      
        1 agagaaagaa cccttggtat tcaaggattc tttttttatt atctgttttt ttatattaac
       61 gatttctatt tggtacattt ttgattaata aaaaggggca aaaaaggggc aacaagttac
      121 agtttttcta tatcttcttt caatttttct tttactcctt ttgtcacatg caaataaatc
      181 ttttcagtga tatcactatt ttcgtgccca acgcgatcct gaatagcata cagaggagtt
      241 cctagttccg ctaattttga aatatgggta tgtctaaaaa tatgtgaact aagtttttta
      301 tcaatcttca tgtcagcttt atgatttctc aaataggtgt taattgctgt tagttggaaa
      361 ggagttcctt tcgtcgtttg aaatagaaat tgaccattcg gatttaattc tagaagctca
      421 ttgtaaatag ctatcgcttt ttttggtaaa tcaatttctc gcattccagc agcagttttt
      481 gtagaatcag attttttcat atcagctatt gaacgttctc tatacatcat cgtcccattt
      541 ataactactg atgcattatt attggtaatg tgtacatcat ctttacttaa tgcgattgct
      601 tctccaggtc tcattccagt caaatatagc cactggaaaa ggagagaata tcttttattg
      661 tgtgaagttg tgaaatctac tagtctttta tattcatcat cttctaaaaa cttatcttta
      721 atttttatcg tttttgactc tcgtttataa tcaataacta cttcgttgat aggatttttt
      781 tctacatagc cttttttcat agcgtatgaa aaaagaagat tcaatttgga tttaattaca
      841 ctgacgtact tatttgacaa gtcatcttta tatatcatat cttcaaaaat attatttaaa
      901 tctactgtat taataccaga aacgatgtaa gtctcaggta tcttcttctt tatagtgttt
      961 aagatattat tcgtaggata atatgtagac tccttaactt gtcttttgta aataactaac
     1021 cattcttcga caagctcatg aaatgttaga tcaggcttct gaagtgtttt ttcgtttaat
     1081 tttatatcaa tttttttatt taactctagc aatgccactt tctgagtttc tcgagattta
     1141 tttttataag taatgctcac tttttttctt tttctagttt ttgggtcaat atatcgctca
     1201 ttatatttat atactttttt tccgttttta tcagttttgg tttcaatcca cattgttttc
     1261 atctcctatt tattgctata ataggaatgg ataagtaagc caattatagc aggtttattt
     1321 ttcatcacgt ccacaaactt tggcgaggga gggggcgtgt ttttttgttt agtagctacc
     1381 tttccatgaa gtatctgcga agaacacttc aaatgttgtt ccatcatttt tcacttcaaa
     1441 atatgctgtt ccttgtgcgg atttacccgg ttggatggtt tccgaaaagt aatctctact
     1501 agctaaatca gatttaatat ccgatgaatc ataaaattct aaataacttg aacttgcatc
     1561 aaaaggtgta gtccctgtgt tttctacaag aaaatctacc tttgcgtaaa ataggccatc
     1621 tggtttatag aaatcatcac cgtaatcttt tgatacagac tggacagtta cgttaataga
     1681 gttaccattt tcagaagtaa aaggaactgc ttctcctata ctatgtgcaa ctgatgccgt
     1741 ctggggttct gaagacgaag ttgtagttgt agtagagctt gaatctttgg attttccatc
     1801 tttatctaag aaagttcgtt gattataata tacaacctgt tcagtttttg gactaaccca
     1861 aattaacatt gtttcatcat cagaatcagt tgaataagta tattggagca tctctaattt
     1921 ttttatatct tttgcactat ttccatgttc aattaattca gagtgctctt tataataatc
     1981 tccttgtgca tcctttgaaa tagatacaat taattcatcc cgtgtgatag cattgtctaa
     2041 ttctttgtaa acaaaatcat ttgtccattt acgtaacggt gaaccaagtg tctgataaac
     2101 ttgagttaaa tcatcaccaa cttttatttt atgaacgtct gatgaagtta gtttttttgt
     2161 ttcatattca gatgttgatg aaacagtatt ttctttagtg ctagcagaat taggactagt
     2221 ggagcatccg cttaatacca agcctaaaaa aagcaaccca aaaacaattt ttttcatttt
     2281 taattcctca cttctatgat atgatttttt tgtaggatct tagaaataag gtttttagtc
     2341 cgtgttgcag cacggacttt ttttacatta tataagaaag cgataagcgc tttctggaag
     2401 tccgtaaaga ttctttaatt cctcaattcg tttaggatat tgatcattgt cttctttgta
     2461 tagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt tgcttctaga
     2521 tgttcttgtt gatacatagt aactggataa gccacgatga aaaatagcat gacctaattc
     2581 gtgagcgcaa atgtagaatc tttcctcaga gtctcgcagt tcatcattta aaaagattat
     2641 cgcacgatct ctaatttctt gaaactgccc cttgggattc tcgataaaag gaacgtattg
     2701 aactttaatg cccatctttt cacaaataca aaaaggatta gcggactggt atttccgctt
     2761 caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt ttgccttttt
     2821 ctttgtcttc tttcacaata tcccagaagg ttccaataag gatatctttt acgcgttgga
     2881 cctgttcagg cgtcagtgtt tctccgccat aagacatatt gacatttgaa tctagtaatt
     2941 tatcaagttc aactacttca tcttttgttg cccagtcagg aacttggttt ctaccgagta
     3001 aataatcagt agttacatta aaataatctg ctacaagttg tagtctttta gtactaggag
     3061 ttctattttt ccattgataa atcgtgtttt taggtatatt taattcttct tccaattgag
     3121 ttatacttat gcctctttta tgagctaatt cctttattct atctagtaaa ttcattttcg
     3181 cacctcacaa gctacgaaaa cactaataaa aaaattagtt aaaggtgttg acaactaata
     3241 aaagtgttag tatactgttt tcgtaagcta aaatattagc taaaaagact tgataactaa
     3301 taaacacttc acggtcggca aactctgaaa tgtaaattac taggcatttc tgtgtcttat
     3361 ttagctatgt ctatatacta ataaaaatat tagttattgt caacgaatat tagctaattt
     3421 tttagcttac agattatttt ttagaaagga gctattttta tgtctgagaa tttagactta
     3481 aaaattcgag cggagatgag aaaaagaaga atgactttca aagaactagc tgcgcttatc
     3541 ggtatttcag gagcttattt atcagatatt ctaaacggca atcgtgatgg aaagaaagca
     3601 caacagcata tcgaaacagt gaaaaacata ttggacatcc gatagggggg ataggcgatg
     3661 acaaaactaa aaaaacaaga ttttgtaaaa aaatacaatt attctccatc tacttatcaa
     3721 cgtcgaatgt cggaactaaa aaaaacagca attttctcag cggcgtatga acgggtcaca
     3781 ggacaagaag tttggatcaa tacagaatta tacgataaat ttttatcttt caaatcctat
     3841 aacaggttac gtacaagaaa ggtaacacct aaagaattta tcgagaagca tttagttgat
     3901 ttataaaaaa cagaacattt tgagaggtga aggttaatgg gtaaattcaa cagagcacta
     3961 gtattcagca cgccattcat catctacgct ttaggtctat ggggtagcag acaagcgttg
     4021 ataggaacga ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag
     4081 tacagagcga aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag
     4141 aatgtatatt actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt
     4201 atttttgata tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt
     4261 ttggtgatta aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga
     4321 tatagcactt ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc
     4381 gactgatcct cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa
     4441 gttagcggat gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat
     4501 gattactttt gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag
     4561 ttgagggcgc attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat
     4621 tattgacatc cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt
     4681 ttaactaaat tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa
     4741 ataaagaagc tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac
     4801 atgttttaag agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg
     4861 tagaaggtaa tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag
     4921 atggtttttt cttgttggct atgggtttta caggaaagaa agctatttac ttcaaacaaa
     4981 aatacattga agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa
     5041 caattgaaga tatgatgatc taccaactag aagaaatgaa agatgttaaa aaagatgttt
     5101 ccatgcttaa agatactatg cgaattagtg ggcaacaaga gtttgaaatt aaacaaaaag
     5161 gaaatataaa agttatggaa gttctagggg gtaaagaaag ccgagcttat gaagaaatca
     5221 gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat
     5281 atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttac
     5341 cagaaactgc gatccgcatg gaaatcgatc aactgaacag acaacaaaga cttttcggtg
     5401 ataaaaatga atagagctga agcgctaaga atagggacgg taattgctaa tcgctggtgg
     5461 agacacaata aaccaatcat cctaagccaa caacatattg ataagcaaaa agcttggcaa
     5521 caaataaaaa agtgactcag ccggcaagca tagagtcaca aaacaaaata tatctaagga
     5581 gaattttagc atatgaataa agaactttcc actttagatc aatatttgac tgattctgaa
     5641 tgggcaagtc gaatatcaag gaaacaaata atcgaaaaat cagacgaaat cttttgacga
     5701 acgaagaact agcatgtgat caagatgatt tggggaattt tgtgagtatt tgggatcatg
     5761 tctatcttat tcatctatcg aagcggtcca gaaaacctga atacatctat gtcatcgaag
     5821 atggcttgat tgacgcgctg gaagaatacg gcagagataa cttgattgat atctcttatt
     5881 acggaccagg caagaagtac attgctgaaa tggaggcaga atttgatgag tgaaggaacg
     5941 aaacgcaacg ataacaaatt attcaatagt ctgtacaaga taaccgtcaa tgatgttgtc
     6001 gaaaaaagaa acaaactaac ttatctgtcc tgggcatggg cgtgggcaga agtcagcaaa
     6061 atctgcgaag aagtagacta cgaaatctat cgtgatccag aaacacatcg tccatacctc
     6121 tttgatgaaa aaacaggcta tatggttttt accagtatca cagtcaatgg agtaaagcgt
     6181 gacatgtggt taccagtcat ggatggtgca aacaaggcaa tgaaagatga gccatatacc
     6241 tacgaagtca atgattatca gtggaataac gaaacgaaga aaaaagagat tgttggaaaa
     6301 atcgaaaagc gagttgaagc agcaactatg tttgatatca acaaaacgat catgcgctgt
     6361 cttgtaaaaa atctagcaat gtttgggcta gggctatata tatttgctgg cgaagatatg
     6421 ccagaagatg tctcgatgct tgaaccagct agccaaagaa gcaaaaagct attcttagat
     6481 gctttacaac tggttgctaa caagtacgat aaatcaattg atgaagcaat tgttgcattg
     6541 actgatgcgg cttctataac cgctgatgac agtaaatgga ccaagagaga cttgggcatt
     6601 ctaaaacgag gcgttaattg gcttgaagat cagtacagag aagaaacaaa agagaagtga
     6661 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggga
     6721 aagacatacg cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
     6781 acggctacga tggtgatcga gttattgaaa ttcgttttat tgatcctaga cgtttcactg
     6841 tacagcaacg aaacttcatc tatgcgctca taggcgatat tttcatcgat acaggcatgc
     6901 caacggactt ctggaaggaa ttcttctact ttcgttttga aggtgtcaca gggcgcaaaa
     6961 taagcttgaa agacgaatcg aatacaaccg tgagtgatgc taatgtctta gcaaatatca
     7021 ttttagattt catctttgaa caccatattc ctttcaaaga aggttatgag attttaccag
     7081 cgaatcaaga gtattacttc tacaaatgca tcacaaaaag agtctgctgc atctgtggca
     7141 aaacaggagc tgatatcgat cactttgaca aagcgctagg aagacgaaag cgcaaagaag
     7201 ttgatcattc agagtacaca tttgcagcac tctgcagaat ccatcatacg gagaaacaca
     7261 aaataggtgt gattaatttc aaaaataaat atcaaatcaa agggatcaaa ttaaaccagg
     7321 aaacaatcaa aaagttaaat attggagggt aaaaatgaca gaacatcgaa gttattatgc
     7381 gattatacca gccaacgtaa ggtacgacaa aagacttaaa ccaaatacta agttgttata
     7441 cggagagata acggccttgt gtaatgaaag aggcttttgt tgggcaggca atgagtactt
     7501 tgcagattta tatggtgtga ataaagagac catatcgcga tgggtaagtg atttgattaa
     7561 gtttggatac ttgaatcggg aaatcattta caaagagggt accaatcaaa taatcaatag
     7621 gtacctacga attaatcaat accctattga cgaaaaacgc aataccccta ttgacgaaaa
     7681 agtcaaagat aataatacat ctattaataa tacatttaat aatacaaaag aatatataag
     7741 agacttaccg ccttcgaaaa aatcgaaggc taagcccatc cgtcataaat acggagagta
     7801 taaaaatgtt cttttgtcag atgagcagat ggagaaactc aaaacagaat tccctaatga
     7861 ttaccaagag cgaatcgaac gactgtcaga gtattgtgaa tcatctggta agacttataa
     7921 aaactatttg gcaactattc gaagttgggc aaggaaagaa aaaagtgaac ctaagaacgc
     7981 aagcagtgga tacaagcgca caggaagacg agagaagctt cctgaatggg caatcgacca
     8041 agaagcctat ctcaagaaaa aagcgctaga acgagctaat agacaatcaa aagcaccatt
     8101 ctaagaggtg gaaaattgaa gatcgattat ctagaactaa ttaatgaaat agcaaagtat
     8161 aaaactggtg aggaaataga aatcctgaga gacgtgtatg aacagcttga agaagctggg
     8221 atcgaaggga ttaagaataa tcgttcgagt tggagtaaac tcagatacta tttcgcactc
     8281 tatatcgata caacacaatt aagaaattta gcttatacaa aattactatt tgttgattgc
     8341 attaaaggat tgcaaaaaca tcttagtgaa cttgagcagg tgtaatcaga tggatctaaa
     8401 aacatttaca gcacagatcg aactaatgca tcaagaagct ttaagacaaa gcgcctcgta
     8461 cgaagacaag tggctcaaca cgttccatgg tggacgtgag agcgcacttg atcaagtact
     8521 caaattattg aaaggggaac gtcgggatgg ataagaaagc ggcaatgaaa cgaatcatcg
     8581 aactgacaca ttctgagaat tggcaagaag acaaagaaat agttacagaa gtccaaaagc
     8641 tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaag attgcaattt
     8701 ggcatggtga tcgaattcta gtaacaggta ctgctgaaca gttatctgaa attactggtc
     8761 tgagcaaaaa catcatctgg gatagagcta ggagcttatg gattgattca aaggggcgac
     8821 agtttaggta tgtggaggag aaaaaatgga cgaactaatc acaaaagtag agcagtgggc
     8881 taaagataag ggattgaacc aggcagatcc aaaagcacaa tttttgaaag tagccgagga
     8941 attcggggaa atcgcttcag caatggcaag aagtaatgat gagctattta aagatagcgt
     9001 aggagacgta atcgtcacgt tgattatcct ttccatgcaa aaagggacaa acgtacaaga
     9061 gtgtttagaa atggcataca acgaaatcaa agggcgcaca ggaaaaatgg tagatggtgt
     9121 attcgtgaag tcgagtgatt tggagggcag caaatgataa aaaagctcat tcaattcagc
     9181 atggatttat atgatatcga atcaggagca acactatctg tggaatcgga ccatctaatc
     9241 ataaattttg gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt actgtttcca
     9301 gaaattgttc atgatttcga agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta
     9361 atggaattga ttgaaaaata cgaggaggac agcgaatgat accgaagttt agagcgtggt
     9421 acacaccatt taaaggtaaa acaattggac aagaaatgaa atatgggcaa gcaggaaggt
     9481 tgatcactca tgctgaaatg gctccagata aatatgtgct tatgcaatcc acaggactga
     9541 aagataagaa tggcgtagaa atatttgaag gggatgtagt aattacatca aaaggagcga
     9601 ttggatatgt tgcgtatctt caacaagaag ctggctttgt agttgtacta aagaaaagtg
     9661 attatagatt aggccatagg aatactggcg aaagttacga tgtagcaact gctcatgaag
     9721 tcatcggaaa tatatacgag aatagcgagt tattggaggt agagtgatta tcttagcaac
     9781 tgactacatc gattctttaa aagatgaaaa tgggaatgtt ccagcattgc taaattgtgg
     9841 aattaaaagt aagaatagaa tcattttgtt acggatatcg gctgatttag caaaatatct
     9901 ttatctgagc aagacagcag tatattacac tgcggctaca cgttatcagt acaagggaaa
     9961 aacaatcagt caggattatt atgatcgttt aatcagtcct gacatgcatg gaaaatcaaa
    10021 atcagcgatc aagttatttg gcgcaataga gatatatgcc gatgattttc caaatttatg
    10081 gttgaaggag gaagcggaat gaaatacgaa ataccactaa gcgaagcaga tgttcagtca
    10141 attattaacg gtcgggaggt taataaaaaa ctttcagatg gtactgaaat agttatcaga
    10201 caaagttatt tgaaagatat ggcagctcct gtattaattg atcgttttaa cgtgactgat
    10261 tctgtggtag agaaccactt aaaagaattt cgatcaagta tagacgacac tttcagatta
    10321 gggagttgat tgacaatgaa caccagacac cgcagagtag caaaactaag aaaacaggaa
    10381 ctgaatgtac taaaggcaaa gtttgaaaaa gaatacggag tttcagtaga agaagcatat
    10441 aaagtagtaa gtcagtgtgt tgctgatgcg agtgagacta ttcgtaagtt tgggatttcg
    10501 atattaaatg atgatcgtaa atgggaggaa atgagatgaa gctaaaagac ggattttacg
    10561 ctagtagtca tggtatcggc ggtttgatgc tagacatgcc gacaaagaat cctaaaacac
    10621 gtaagaaacc aaaaatcaaa gtcggtgaca tggttcgctg tgaagcagaa gggttcatct
    10681 atccatttcg tggatatgta gagcatgtct ataatcactc agcaatcatt cgcattgaaa
    10741 acacgatgga atgtgacaag tggttagcga aaagcaaaga gaatttagca gtggtgagat
    10801 tggtggatat ggaggttata aacaatggaa ttaaaaattg acaaggtaac gtttgaagaa
    10861 ccagcatttc aagaaatgga tgaacgaatt gcagaattgg tcgagaaaaa acaagatgaa
    10921 ttgattgctt tgctagaaaa gaaataccaa tttccgaaat atatgaacaa aatgcaagca
    10981 gcaaaatatc ttggtgtaag ttacaacacg atgatggata agtacgtacc agctggtttg
    11041 aaaatgggta tcgttaacgg cgttattcgt atcagcaaag aagaatgtga tcgttttatg
    11101 aaagaacaac aaaaataaaa taaaaaaacg tttagcccct cagctaaacg cacaattata
    11161 atttacctga cagttaatta taacaaaatt gcggaggttg aggaatggcg gaatgaaaaa
    11221 gacaataaaa acagaagaag catatattga agtatttgac acaccagcat gctaagtcga
    11281 gaagtgtaac tcggcaaata aaaaagccgg atcgctccga ctgatgtaat aaatccgaca
    11341 agtttattat atcacataaa aggagcggtt tgacttgatg caattgttac gagaggtaga
    11401 tttcaaacag acaagatgca atgcgagaga tgtgctgaaa aactttcggc gtttggagcg
    11461 gatggcaggt cgctctttga tagacattaa gtcgccgatt attacggata tgccgaaggc
    11521 accgaagcat ggcaataagg cagaagacgc gatcattcag atgatggata tagaaacaga
    11581 gagagatgcg attctagcgg ctttgatggc gcttagtctg attagtcgtc agatactcta
    11641 ctacagcttt tgtgtgccag atagcttctc aaactacagg attagccgtg aagtgggtta
    11701 ttcagaaaga agcatacaac ggatgaagtc ggaagctctg atagagtttg cagaagcata
    11761 taaacacgga agaataattg cttataaata atttggcggt tttttggcgg aatgatggcg
    11821 gtttttagcc atttatcagt gatattatga tagtgtcgaa agattaggaa acaggatcga
    11881 caaaataaaa tgtaagggag gaaatctccc tcatcgttta attaagcttc gataggcagc
    11941 aacggaaata ttaagaataa ggatgtgaat ttcaactcct tctaaattgt tcttattatc
    12001 tatcatccgt tgctgtctat taatttatgt attggagggt aagaatatga aattatcgat
    12061 tgaaggtact tcaggagaaa taaaagaaat cctctcagct atttgtaata gccgagagat
    12121 tagcaattta atcaatatag ataatttaaa tgaatctatt gttactccaa gttcccgaga
    12181 gaaattgcag aaatctgatc agcaaaaaca actaaaaatg ggatattagt agttctctcg
    12241 cctatgatgg taacatcttt cagaaagata gatttgacta ccaaactttg ctcatcatct
    12301 ttttctatat catcaatttt ttttgagcga aattctgcaa aagaatccat catagcatca
    12361 ataagaggat ttacttttac ttcagcatca aagtctatag gagttccgca aattgttgct
    12421 cctccagttt gaataataat attattgtta gaatttttag cgaagagttc aatatattca
    12481 atcatgtctg aacggtaatc atcaattttt gccatttttt taccacctta aattatttca
    12541 gcggaccact cgctgataac taaaattata cgcttagtat ttattttcac aatattaatt
    12601 tgtcactgtg gcggaaaggt agacgctaag catgcgtgct aggtcaatgc ttcggcaacc
    12661 atgcaatgtt cgattcattg ccagtgactt aaggaaccta cggaaacaat tcatcttatc
    12721 ggatgccgat gaattggctg actagtcggg atgccactag cagttaaaag gcataaaata
    12781 ctagcgcaga cgtgcgccac tctcaggtgt aggttaggag agaaacatta attggggtta
    12841 ttaggaatac gataacctgc ttgcgacaaa gctttgtact gtcgcgttgg tcatggacag
    12901 agacggtatt ctgtttcagt attcgttagc aaccgaagaa tcggcggttt aaaaatatag
    12961 gggtgcgcaa tttcgtacgc gttttgtgca tcgtgtaggt tgctattaca tattagatca
    13021 ctcatcgagt gatcttttta ttttgaaagg ggagtaaaca aatgaacgaa aaccaattaa
    13081 gagagttgtt taaaacgaat gaagcaaaca aaacaatgga ggcgacattc tacgaaactc
    13141 aaaaaagctt agcgttaatc gcaaaacaat caaagtattt ctacgatcag cttattctgc
    13201 aaggatttaa tgaaggacag gctatggaat ttatgatgcg aaccttttct gccagtaacc
    13261 aacagaaaga gtgatacata atgagaaact actggtatat atcgctaact aatgaatatc
    13321 ctcgaaccat tgatgattgt tcagtgcgtg ttgtgcgttc tgtacaaatc aaaaagaagt
    13381 actccatcat tgaaatgacc agagaagcta caccaaatga gatcgataag tacaatcttc
    13441 gttactgtgg ccatggatat tggaaagacg aatatattca acaaaacatt gagaggtaca
    13501 tcaaatgaat gataactatg attacatcaa gttgattgaa aagataagag cagaaaaaga
    13561 tatggacgaa ctagctaact tattcatgaa catcattagt ttggtggggt taaagatgga
    13621 tgaagtagcg gcactaaatt atttcatcgc tgagcaaaca attagagcag agcacaatgc
    13681 taagttcttg aaagataggt tagatctaga tgtaaaagga ttaggcgttg aagggatatt
    13741 caaagtgcaa gaagccttag tgaatgttta tgtcgaaaag atgcaatagc attaacaagt
    13801 aattattgga tgttttcagg agggaaacta tgaaattaaa agattatatc agggaagggt
    13861 ataacattgt aactacacca aatctagctt acaaaattca agaagattat cccaatgttt
    13921 tagtgtttac tgatagagct ttagatgcca ttcctatagg gaagttatta gtagatcgtt
    13981 attatagcaa caatccagct gttcttagtg ctaagccact tcaaaacgct tatgctattg
    14041 aacgctttac ttgcgagttt actggatatg agacggtagt accaatgaca ggcgagacag
    14101 gaagaaaagt aattcaacag atcaaaaaga ggattgctga attgacgtta gatgatggaa
    14161 gtaatacgaa ccttctagaa atcaaactaa gagatactga ctcagtacca gaagtttatt
    14221 ataaaggtca acgaatggat gagatgccga taggtcttgt cgatattact tatcattggc
    14281 atacggaaga atatgaatgc caagtaccaa aaggagctaa cgacatcact attgaatatt
    14341 atgattcaca taataacaag tacttagata gaaaaatcat tgggcacaag agagatatgt
    14401 aaatgattga tgtttcaact aaacaagcaa gagctaggtt ctatggatca actgagtgga
    14461 ggaaacttag aagtcaatgc ttagagcgag atcattatga gtgccaatgg tgtaaagcag
    14521 agggtagact aacaactcaa catgattcaa ttcttgaagt ggatcatata aaggagctgg
    14581 agtatcatcc ggatttagct ctagatataa acaacctaag aaccttatgc aaagattgcc
    14641 ataacaaaag gcacaaccgt atgaaatatc gtggccaaca aaagaaaaga aagtgggatg
    14701 atgaatggtg gtaagaaaaa cagcaaacag agatgatgtc aaacgagcag aagcaatcgc
    14761 tatggttgat aaggctattg ataagttggt tctatttaag agtcagctca ttgaagaaac
    14821 aacaactgta gaggatatat ctatcaacaa tagactaata ggtaacgcta atgaagtaac
    14881 agggacattt aatattaatg tagacattga ttataccttg agtccatttg cacatattgt
    14941 agaaagcaac gagcaagacg gctgataaat cagtataaag cgaagatata tagagtgaaa
    15001 tacccccggt caaaagattt tgagaagaaa taaaattttt gggaaccggt ggatggggtt
    15061 aattctgcaa atgcaacgta tttattttac ccctcccccc tccgccgccc ctttagtaaa
    15121 tatagaagga aggtgattgg tttgaattct aagttggttg gcacgagaat gaaacaaaaa
    15181 agaattgaaa aaggattgac acaggctgat gtagcaacga aaagtggtat ttctagtagg
    15241 tattatggaa gtattgaaaa tgggaaaaat tcccctagta tcgaaacgtt aaactcaatt
    15301 tccatcgtca ttggatgcag tttacatttc ttactcaatg atcgcatgga cgacatggaa
    15361 aaacgcgtag agctagaaaa gaaacgtctg cagaagatat tcgataaaat tcctcatgac
    15421 aaacttaatt tagttgaagg gctgatcatt caggcagcaa ggttacgtgt cttgttggat
    15481 gataactgga aggacatcat tgaaaacggg gagtacgaga aatttaaaca aagcgagaac
    15541 caaattgctt atgatagaaa gcgtcctata gttgaaaatt atgataatcg agataaaact
    15601 tatcaagcca tcgtcaaaca actcaccgat ttattaccgc aaggcacaag agcggacaag
    15661 aaaagtaagt tactcggtag gaaataaaaa tgctttttaa ccagcacgtt gatcaatata
    15721 ttcgtaaatg gaaatctggt gagttaatcc ttaacgaaaa aaggatacaa cttttaaaac
    15781 taatagaaaa agaaatatta cctcgcgacg acatttatta cttcgacgag gagcaaataa
    15841 ataattatat cgagtttagt caagcatggt attttgagtt agatgaatgg gagaaattta
    15901 tttctgcatt catttttttg ttttataaag aagatgacga agttgttttt gatgaatttg
    15961 ttattaatat ggggcgtggc gggggtaaaa atggctttat ttcaacattg gcaaattatt
    16021 ttgtcagttc gcttcacggg attgattact atgatgtttc aattgtagct aatagtgaga
    16081 aacaagcaaa aagaagtttt caagaatgct atcgagtaat caataaaaaa ggaaatgaag
    16141 atttaaaaga agagtttgaa gcgtataaaa gcagtctaac cggtttagag acacagtctg
    16201 tttttgagta caaaactagt aatgcaagct ctcaagatgg cggtcgagaa ggtgcagtaa
    16261 tatacgacga ataccacgaa atggaaaaca cagatattgt agatgtgttt tctggtggac
    16321 tggggaaagt tgatcacgga cggcaatttt tcattggtac taaggggttt gttcgtgaag
    16381 gttactttga tattaaatat agagagtgtg aagatatttt aaatggtttg tcagagttca
    16441 agggtgtatt tccgtacata tgcgaactgg atgccattga agaaatggat gaccccaaaa
    16501 attggccgaa agcaaacccg gcactgcaac caccgttgaa taaacgtggg aaacgtctgt
    16561 ttaacgaagt catgaaaaag tacaaaaagt tagctaaaga accttctgga cgctcggcat
    16621 ttgttactaa acgaatgaat tttcttgaag acaatatgga aaactctgtt gcttcaagag
    16681 aagaaataat ggcaacaaat cgtccatttt ttgaactaga tacggtaccg attggatctc
    16741 tcgatttcgg tagtgttcga gattttgcta cttgcggttt gctatttaaa aatcgaaaga
    16801 aatcggaata cgcgtttaaa tcatttacgt ttgcaatgaa aaatttttgc gacgttcatt
    16861 atggatactc gttgaaagag caattggttg gcagtgaaaa acgggcacca ataaaaactt
    16921 gggaaaaaat ggggctgatg aaagttattg acgagccatc tttaaatcct aagtatattg
    16981 ttgattggtt tattcaagct agagaagaat atggagtgag agtaattgtc gtggataact
    17041 ataaggcaga tattttaggg cctatgcttg aaaaagaagg atttgaagta gttcgactaa
    17101 aaagaccatc tagtttacat ccattattag ctcctcgtat agaagatgga tttgcaaatc
    17161 acaaatttat ttttggagat aatccgttaa tgcgttggtt tactaataat gtgtacgtaa
    17221 aggaaacgca agcaggcaaa caattcttaa aaaaagagga agtcaagcgt aaaactgatg
    17281 ggttccaagc atttgtacac gcattgtaca aagctacaga attagatgaa cagattgatt
    17341 acgatgatgc ttttgatatg ttggatgaga ttgaatttta aggtatactt ttctttacaa
    17401 aggggtgctt tcatgaggat aaaaggaaaa aacatatctt gggtatttta catgttgggg
    17461 attttagctt ttacatttgt ggcaataaaa ctatcagaaa ttattattcc aaatgacagt
    17521 gtagtttcaa agaatttggt tttagaaaga gctaaactaa ttatttcaat tttaggacca
    17581 gcattaacac taattgtttt tgttcgaaca attcaaactc aattagaatc ttcaaaaaac
    17641 ttaattaaag ataacgtcag taaagatttt tataatttgc taaatttatt caaaagtata
    17701 caggataaaa tgaattctga attatttagt acaattgaaa aaagaattaa agtacctata
    17761 aataatataa atacaaattt agatgttaga gaaaataaag aaactactaa agaagtacag
    17821 gaggatttaa aagaatatag tgcatcattg aataaagttt taagcgaaag cgttaaaaaa
    17881 attaatgaaa tatttgagga taactacaaa gacataggta gttatctaaa agtattccat
    17941 aggattctta aaatactaaa taacaggtat gaatcaaaag atatttcgca tgaagaatac
    18001 aaaatgtatg ttggcatatt acgtagtcag attgaagatt acgaatttta tataattctt
    18061 ataaattctt tgtttactct tcgcggaacg ggtatgggga ttgagttaat tgaaacatca
    18121 ttctttggtg atgaactaga tattgattta aaacagcatt tcgaacatcc agagttttta
    18181 actgaaatca ttaaggataa ttttgttgaa aagaaatcgt atcgtgaatc agtaaaaaca
    18241 agaaaaaaaa tagaaaacta tttgaaaata gaaagataaa tgataactta ttatctcttt
    18301 tgagaataag atacacgtta caagatggaa ataaaattat tataagtaat ggagtatagt
    18361 cctagaaata gggcttttta ttttaccttg aaaggtggtg aaaatatgtg agtttatttg
    18421 atatctttaa gcaatcaata aagaacgaag atccatcgga ttggattcct gactttgtgt
    18481 ttggtgatga agaatctgct agagcatatt tgaaaatcat ggctaaaagt acagttcttg
    18541 attttgttgc aagaactatg tcaacgttag aagttaagtt taaaaataaa gatgggacat
    18601 cagattggga ttacattcta aatgtccgac ctaattctga tatgtcagca gctacatttt
    18661 ggcaaaaatt tttttatcgg ctcttagatg acaacgaagt gttagtgata tttacagatg
    18721 ataatcaatt gctgattgct gatgatttta gtcgaacaga gtatgccgtg tatgatgatg
    18781 tttttacaaa tgtgatggtg aaaaattttg tctttgaacg ttcatgggcc atgtctgacg
    18841 tcatttatat ggaatataat aacgaagaat tagaaaggtt tacacgaggt ttatttgaag
    18901 attacgccga gttgttcgga cggattctag aagttgcaat gcgcaataac caaattcgtg
    18961 ggtctgtctc cattgattca acaggaacgg caaatgatga aaaaggaaaa gatggtaaga
    19021 ctagatctca aagattacaa gaatatattg ataagatttg gaactctttt aaaactaact
    19081 cagttgcaat tgttcccaaa gtgaaaggct ttgagtatga ggaatacacc aataaacaag
    19141 gcgtatcgaa tcaatcacta gatgaactga ataagatgaa gtcgtcttta attgccgatg
    19201 ttgccaacgc cataggagta cctacggcgc ttatttatgg cgaaaaagct gaactagatt
    19261 caaatattaa agcttttcgt aaattgtgta ttgctccact agttaaaaaa cttcaggacg
    19321 aattggtggc aaaaataatt agtcgacaag agtacaaaaa cggggaacgt atagaagtta
    19381 aaaaagttct ccaaaaagat ctattagagt atgctactca aattgacaaa ataacatctg
    19441 gtggtgcgtt ttatattgac gaagttcgtg atgaagttga ttatgatgag ttgccaaata
    19501 atgaaggaaa aattcgtatt cgtacgaaaa actatgaaga aactgtgaag ggaggtgaga
    19561 acgacaatgc cgaaagctaa aaaagtacca tttcagttta ctaacgaaat tcagaatggt
    19621 aagcacattc tcaccttgag tggaaatgta cagaaaaaat attggcgtga tgatgatgtc
    19681 ataaacgcta aagatattcg tgactctcta gatactgtaa ccgatgatat tgtaatcaag
    19741 ttgaacagcc caggcggtga tgtgtttgaa gggattgaga tttacaatta tttaaaagat
    19801 cacccatcaa atattactgt agaagttaca gggcttgccg caagcgcagc aacgtttatt
    19861 gtcgctggtg ccaatgatgt gattatgaat gttgggactt ctttgatgat tcatgaagct
    19921 tctacatttg cttgggggaa taaacaggac atccaaaaaa cgttaaacgc attggaaacg
    19981 atcgatgatt ctatcttagc tatttattca gataagactg gccaatctgt tgataagttg
    20041 cgtgaatgga tgaatgaaga aaaatggttt acagcagatg aagctgtcaa atatggattt
    20101 gccaattctg tgaagcgtga agaaccacaa aacgaacctc aaaatattgc agcgatgatt
    20161 caagatgctg ttgccgttgc aatagcgaat ttaaaggaac ctgttactaa taaaatcgaa
    20221 caagaatcaa aacaaaaatc attaatcgca cgattgcgaa aaggagaata aagatatgtt
    20281 aaaaattaca gataaaactg cagatgcaaa aaaggcattt aatgctattt cttcgaaaga
    20341 agatgcaaca cccgaacaaa ttaatgctgc tctagaagaa tatgtaactg ccattgcaga
    20401 agatgccggc aaacaggtgc gtgctgaata tgaagaattg aaaaatgtga cggacaatcg
    20461 tgtattagag gctcgtggaa ttcctacact aacagtagaa gaaacaaagt tttataatga
    20521 agccgtaaaa tctggaggtt tcgattctga cgtagtttgg ccagaaacga ttttggaacg
    20581 tgtttttgaa aatcttcaaa acgatcatcc tattttaggc ctcattaact ttacaccaac
    20641 tgtaggtcgt gtcaaagtaa ttagagcacg tcgtaaaggt gttgctgttt ttggaccatt
    20701 gcataaagac cttgaaggcc aactagatgc tgaatttggt gcaactgaat ttgttcaact
    20761 tgcgttgacc gcatttttct taatttctaa tgatacctta gatttaggac cacgatggat
    20821 tgaccgtttt atcaatcttt ctttaagtga agcagtacgt gatgtttggg ctaaaaaaat
    20881 tattacaggt tctggtaaag atgagccaat tggattgctt aaagatctag atggtgcggt
    20941 cacaagtggt gtatatccag ataaagctag cgcagggaca ttaacttttg cgaaagagaa
    21001 gattgttctt gaacttgcag gggtgatgaa aaaattatct aaatacacat acaaagtaga
    21061 tggtgacgat gaaggtgaag tggaataccg ttctgttgct ggacgagtgt atctaattgt
    21121 taatcctgtt gattactggg atatcgtagc tagagttaca tttgcaaatt taaacaatgt
    21181 gtatgggact acgatgccat ttatcgctgt tgatcatatc atcgaatctg tagacgtacc
    21241 agaaagtaaa tgcattgcat acgttgataa agagtatgaa gctactcaat caagagctga
    21301 aaaagtctat gtatataaag aaacttttgc aatgaaacgc gcaactttgt atgctgtcga
    21361 catgcttggt aatggctatc caacaaataa tgatgcagca caagtatatg acttggactt
    21421 tacagcgggg gagtaacgtc ccctgcaatt ggtgagataa caccgacgac tgatggggca
    21481 gtcattaatc tttcgtaaat ggggttagag tatgaacact gaagaattta taaaagaata
    21541 taaggcccga tttcgtattt tccatacttc cgaagatgag gatatcggga aacagttaga
    21601 gagcgggttt gccgatataa aatctctaat cggcagtttt gatccttcca cttatgagaa
    21661 aggaaaagaa ttagtttatg aacgtactcg gtatctacgg aacgaatcac tagagtactt
    21721 ttacgacaat tttcaaacaa tgattatgga tgcatcaatt gatctggcag gtgctggata
    21781 tgccaattaa atcaaattat aagaagccta aaattgtagc tggtgatcta aatacgccag
    21841 ttactttttt tgagtttcaa cctaataagg gtccagaacc tggtgatgaa gaaaaaaagg
    21901 aactgtattt ttgtacagca ctagtttaca acccatcaat gaaagatcgt gacattcttg
    21961 gcacaaaaga aggcgttacg ataaaaatac gggatcccca tcaatcttat attcctacta
    22021 acaaacataa agtaaggata ttagattatc gatatggaga taaagtttgg gaaataatcg
    22081 atgtctcacc ggatttggaa gaaaattcaa ttgtaaagat agtattagaa ttaattactt
    22141 aaatttggtg ataaacgatg agtaacgtaa agtttgaagg cgttcaagaa acaatccaag
    22201 ctttagaaaa aaagtttgga gaaaagaaaa ctaagacatt aacaaaaaaa gcaatcaatg
    22261 ttggcgctga aaaagtagaa aaacagttgc aaacggacat gaccgttttt aaagacaaag
    22321 gttatacgat tgatgaggtt gtacgtaaga acgctacgta tagaaattac aaggcagaag
    22381 ctgaaatcgg ttggaatggt cctcatcagc gttatagaat cattcactta aatgaatggg
    22441 gatatacacg aaaaggtaag caaattaggc caagaggttt tggcgtaatc actaaatcgc
    22501 ttaaaaattc cgagccacta tattttgatg ctgttgggga ggaggttaaa aagagattat
    22561 gaaagatatg ctcaatatca tttatgaggc tctttgtttg aatgagtata ttcgttcaat
    22621 gacatataac gagaataccg agcaatatcg tattaaattt tatgagcaac ctgaaaccgc
    22681 cgataaaaca gggccaatga tcacacttcg tcctgtagat acacctaacg aagcatatca
    22741 cggaagtaac aaagagcttt ctgtagagca tatggtacag attgatgttg aatcgaaata
    22801 tagagcaaca tgcaagcaga ttcaatatga gataaaaaaa gagatgaaaa aactaggctt
    22861 tggccaaatt tctggtcaag ggttagatga atacttttct gaaacaaaac gctttgtaga
    22921 cgcgcgtcgt tacgaaggaa atacaaaaat ttacgatacc gaatattaga aattaagaca
    22981 cgaaaataac gtgtcttttt ttattaccca aaattaggag gaaaatttta tgactttagt
    23041 aggattcaaa aaaatgacaa tcgggatctt tgacagcaca gggaagattc cagcagcaaa
    23101 tttatatgta attgaaggtg aacaagacaa aggggcgact gtatctgctg aaatcagtgg
    23161 gctatcaaaa gaaccatcaa aagtttacgg atcgaacatc gcttactatg tctctcaaaa
    23221 aggaacaggc gatgtttcag ctacttttgg attgttagat ttaccaacag aagtaaatga
    23281 caaaattctt ggttacaaaa ccgatacaaa caaaatcagc ttcttgggag aagatactga
    23341 gccaccatat tgtgcaattt tgatggaatc ggaggatttg aacggcgata cagcaatgct
    23401 tgcgatgttc aaaggaaaat tcagtcgtga atctattaat cttaacacta cgacaaatga
    23461 agctttcgaa ccagaagcag aagaatatgt attttcagcg attgccaatg atgttgaagg
    23521 tgatgctaaa ggccaaacag tcgcaaaata tgttggagac gaagaagctt ctattactgc
    23581 tttgaaagag atggtatttc ctgcagcggg ggagtaacta gctccgttgt tggagcaatc
    23641 actcccacta ttaatggggt aacagttgaa ttaagttagg aggataacta tgccagatac
    23701 atttaaaatc tataaaaaaa atggtactga atttacaaaa gtcgcagagg gtgaaagtcc
    23761 actttctatt actggaattg cagcaaatac tcaagttgct aaaggagact accaagctac
    23821 gcgtgtggtt ggaggtcaag aatcagtaaa agtagatatt cctgcattta ccactcttcc
    23881 aatttcagtt actggtgtga cgttggataa aacgacagct gaagtcgaac aaggcggaac
    23941 cttaaaatta actcctactg taacaccagc aaatgcaaca gacaaaacag gaagctggag
    24001 cagttcgaat acagctgtag ctactgttac tggaggcaac gttacagtaa aaacagacgc
    24061 tgaagttggc ggaactacag aagtttcatt tactactacg gatggcggta aagtagcaaa
    24121 atgtactatt acagttattg aaaaagcagc gggctagtct tcggactagc ttttttattt
    24181 ggaaaataat ggaggaatct aaatggcaaa agtaagaatt gaattgaaag aagaatcagg
    24241 aaagaaagtc tatgaaaaca tagacacaac aggtaaagat tatcgaaaag ctttagaaac
    24301 aatcaaaaag ctaaatgaag atggagtaat gatttggaat cgtttagaca tttatttgga
    24361 ttttgctgta ggtatcttca gggatagcaa attgacagct gaacaaattc ttgatggctt
    24421 accatctgag aaggtaatga gcacattgga cgacatccta ggagaagtaa tggggattga
    24481 aagtaatcca gacccagacg caaaaaagta acacccgaag aagcagaaga aatgtactta
    24541 aacctatgca gagagttagt gaaagcggga tggtcactat ctgacataga agataattcc
    24601 tttgaaatga tgatgaaaat tgcgtgcact tcgcctaaga aagagaaaca aaaagaagtt
    24661 gatcttaaag acttcttgaa atccatgtag agaggaggta aacatatggc aaatggaaaa
    24721 cctttaggca atatgattat aaaactagac ttagacagtt cagctttttc aaaaggtcta
    24781 gccggagcta aaaacgctgt taaccatcaa atgaaagcaa tgaaatcaca gatgcaagta
    24841 atgaactctt cagggaattc tctcggtgct ttacaagcaa aatataatgg tttaggcgga
    24901 gttctacaag caaatgaaaa gcaagtagaa ttgctcacta aggcttataa ggatagtttt
    24961 gactctaatg gaaacgctac tgcttcgact gctaaatacg ctaatcaatt gaatcaagca
    25021 acagctagat cagcaagtta cgaatctcag atgaaaacaa ccgttggaca gattgctcgg
    25081 atgaaagtgg aaacggaagg gattacgggg aaactaaaag ctaattctga tgtattgatt
    25141 aattctggga aaaaaattga agcctttggt aaaggagttt ctgggatagg atctgcccta
    25201 actgttggag taacagctcc aatagtggca ggtgcaactg ctgttactag tgcagctatt
    25261 tcttgggaaa gtgcttttgc aggagtaaaa aagacaaatg atgaagttgt agattcgaat
    25321 gggaacgtag tttactccta cagtgattta gaaaaaggcc ttcgtgattt agcgactaaa
    25381 ttgccagcaa gccaccaaga aatagcgaac gttgctgaag cagcaggtca attagggatt
    25441 aagacagaaa atgtagttag tttcaccaag acaatgattg atatgggcga atctactaat
    25501 atgtcagcag aaacagctgc aacttcttta gcacgatttg ctaatataac ccaaatgtct
    25561 caaaaagact ttgataaatt aggatcagtc attgttgatc taggtaacaa ttttgctaca
    25621 accgaatcgg aaatcactga aatgggactg cggttagcgg gtgctggtaa acagattgga
    25681 atgagccaag gcgaaattct aggatttgct actgctctat cctctgttgg tgtcgaagct
    25741 gaagcaggtg ggtctgcctt ttcgaaggtc atgatccaga tgcaactagc tgtagaaaaa
    25801 ggatttggcg cttttgatca gctgaaacag atggcagaag aacaaggtgt tccgtgggtt
    25861 aatttagtaa atgctgtgag agacggtgga aaatcgttaa aagcagtatc agagcaaatg
    25921 ggattcactt catctgattt aaagaaaatg tataaagaag cggacaattc gaaaagttcg
    25981 cttgaaaatt ttgcgaatgt ggcaggtgtt acaagtgaac aattccagaa gttgttcaag
    26041 agtgatcctt cagaagcaat catcaagttc attcaaggac tgaaagattc agaaaaacat
    26101 gggacttcag caatcaaagt tctggatgac atggatataa aagaagttcg actacgcgac
    26161 agtttattga gagctgcaaa tgctagtgac atattcaaag gagcaattga aaaaggaaac
    26221 ggcgcgtgga aacaaaatac agctttaaca aatgaagcta ataaaagata cgaaacaact
    26281 gaatcaaagc taaagatgtt gaaaaacgaa gtgattgata cagcaatcga tcttggaggt
    26341 ccatttgtag acgcgttgcg tgatggtcta aatgtatcaa aaccgttgat aaaaaactta
    26401 gggaatatgg cgaaagcttt ctcagccatg agtgatgatc aacaaagaaa cattatcaaa
    26461 tgggttggat tagcagcagc agctggtcca gcgctgaagc ttttaggaac tggtacttca
    26521 gtaataggta aagttacttc aactactgga agattgacga aaagccttgt tgatttagca
    26581 gcgaaagcag cagaaaagaa ggctctggat gcctttgcat cgtcggtaac aacagctggt
    26641 gctgcagctg caaagacagc aggagctgga ggtattggcg ggttagtccc tatgatggga
    26701 aaaacagcta gtgctgcaag tactgctgcg ggcgctagcg gaataggcgc aatgacaaca
    26761 tcactaggtt tgttaggtcc tgctctactt ggtattgttg gcgttggtgg cgcactagca
    26821 gttggctatg gagcatggaa attatttggt gaagaagctt ggaattcctc gcaaagagta
    26881 aagcaatggg gaacagatgt cggtgaggaa atagataata ctcttgacgg agtacagaag
    26941 aatattgaag gtgcaaatgg tcaattcggt cttctcaaag agggttttac tgttgatgat
    27001 gcgaataata tggcggctaa tttcgcaacc gctggaacat ctttggaaac atctttaaca
    27061 ggtagaataa aggcattaga cgatgcacta aaagggctac ctcaatcagt tcaggatgcc
    27121 atgaaagaag tagctgaatc agaagaagaa accttagggc aatctctttc taaaataaaa
    27181 gacaacaatg aagaaataaa aaaaatcaga gaaagagccg caaatcagaa cagagacttg
    27241 actgtttcag aactacaaca gatacaggat ctgatgaaag gaacttctca agcgtatgtg
    27301 gatacgctcg atgtttctgc taaagacaga aaaaagatac tatcagccat gaatggtgat
    27361 gttgcacagg ctacagaaga agaagcgaag acgtggttac aatctttagc gaaacaaaaa
    27421 gctgcaacta ccgaaaatta tagacaaatg cttgaagaaa aaaaggcggc tttaaaggaa
    27481 aagggagtaa gcaaagaatt cattgatgca ttagaagatg aaatgaatga ttacatcaaa
    27541 actacaaatc aaggattcga tagccaaata gctgctataa caaataaata tccacaattg
    27601 attaacgaag tgagtttatc aaatggtcaa ttatttagca aaacggcagc tgctatggac
    27661 aatactggtc agtatacaaa agtttttctc gaaagtaatg aaaagatttt agcccaagcg
    27721 aagaaaacat cggatgaaat agcagataat gccgagaaaa atgcagaaca acttggtatg
    27781 gtagctgatg aggctacgca tgctggatca acttggaatg gattagtttt agacgaaaaa
    27841 acaggaaaag taaaaactaa cgtacaagaa gtagtgaatg aagcaacgaa ggatttcact
    27901 aaatggaatg aacttaaacc tgttgttcat gatgcgaaac taacaacgaa cgcaaaagat
    27961 gtcatttcca tagcggcagt acaaaatggt tattgggatg atatggaatg gaaagaaaaa
    28021 gaagcattat tagaagatga gtgttctaaa aatgtataca aggctttaga aaatgctggt
    28081 aaatggaatg agttagatat tccttctaaa gaagcaatcc taacttcaaa tactcctgaa
    28141 gttatgggtg aaactctgat gaatttggga ctatgggata cttacaaact tcaagtgaaa
    28201 gatttagatt tgaatgcgta taaagtatac gatgctatcg atggatcaga agaaaaatta
    28261 aatagttggg ctcaattaga taaccccaca aaagaattgt tattagataa ctcagatttt
    28321 tctacaaaac tttttaattc cgaagaaatg tttaaacgtt ggaatcagtt atcatctaat
    28381 gcaaagtttc tttcagctaa taatactaaa tttacagagg ttcttttatc atctgacaga
    28441 atgtggaatc aatgggtaaa ccttcctgat aatgagaaaa gattacttgc agataacgaa
    28501 gatttagcta ccactgtttt atcttcagaa gaaatatacg gaagatggac tcaattacca
    28561 gataatcaaa aatatatgtt agctgacaat actgacttag caaacaaaat attcgcttca
    28621 gaagaatcat ggaatgcatg gaaagcaatt ccagacacag ttaaaaactt gcttggtaat
    28681 aatattgatt tactggctaa agtgaaagac gggactgtaa gtgtagaaga atataacaaa
    28741 gttatgccac ttcttaaaac tctatatgga gataacgtta atttaaaaga ggcaattagt
    28801 ggggctaaaa gctctataga gaattacaac aaaaatattt ttcctaatga taaaactgca
    28861 gtaggacacg atgaggcttc acaagctgct aaagatgcat ttgaagcgtt caacatcttc
    28921 caaacaaaaa taccagataa aattactaaa actgtatcag ctgactttat aggtcctatg
    28981 cctaatgcta aaggaacaaa cttccatcct ggcggagcag ctatggtaaa cgatcaaaaa
    29041 gggcctactt atgaagagct tataaccttg ccaagcggag aagcttttat acctaaagga
    29101 cgtaatgtcg ttttaaactt accgagaggt tcgaaagtac tgaacgccac taaaactaaa
    29161 cgtctagtgc ctaaatatgc tgatggtata ggaaacataa cgacggtttc atctacgcta
    29221 aacttagatg ctttaatttt agctattaac gagctaacga cggtattgag aactcaattg
    29281 acggcaggaa cgactaatgc taaagatact aaactatcag atgaaactaa agttgccgat
    29341 ccgattattc cggacagtct ttcagaaaaa tcagatcagt atctaggaat tggtgctcaa
    29401 tggttaacta atctaatgaa tggttggaac tcagcggttc ctcaatatat gaatagtgaa
    29461 atggttttta ttacaaacta tctcaatgcg ctaaaacttc aaaacaatcc taattatacg
    29521 caaggagtag catggaataa gaacttactg aatggatgga atagtttaac tgggacattt
    29581 atttctacga tcaattcatt ttgtaatcaa gcaatggtca cgcttagaaa ctacaacact
    29641 cctatgtaca acaatggccg aacgtggcag caaaataatc ttaatggttg gaattcattg
    29701 tatggatcat tcatagcccg tgtaaatcag cttggtaacg attcgattaa caaccttcgt
    29761 tctaaaagtg gtggttttta caatgcaggg acattcttgt tgcaatcttt aataaacgga
    29821 atgaattcgt taggtaattc tctttctact acaatgaaca atgtcgctaa tacaatggtt
    29881 ggtggtatgg gcaaaggggt taatggcgtc atatcgggag ttaactatgt gcttaaggaa
    29941 gtcgagtcaa gtaagaatat cggaaactgg ccaattccac aatacgctaa gggaactgat
    30001 ggacatcctg gtggacttgc tatgattaat gatcaaaaag gcctagttca cgaagaatat
    30061 gttcagatgc cagatggacg tggttttatt gccaaaggta gagaccttct tgttaatttg
    30121 cctaaaggtg ctcaggtctt aaatgcttct ttaacaaaga aattaaagga acgtttaaat
    30181 attccacact acgaaaaagg tattggcaat ttagatattg ttgatttgtt agatgatgaa
    30241 aaaaggatgt tagaattctt aactagcaaa gttgactttt caggcattaa tgaaccatgg
    30301 cttgatatga ctaaatcagg aacgtcatta atgtccaaag ttgcaaatac aatgcttcaa
    30361 tcaaaattaa gtgaattctt cactcatggc aattttgatg gcgaagtaaa tgccaatggt
    30421 gtttatcaat atttagttga tgttgcacag aaagtaatgg gtaagttccc cggacttaca
    30481 gtaacttctg gatatcgagc aggagatgcc tactatcacg gaaaacggca agctatcgac
    30541 ttggcttatc caggtatttc tggagatccg agatatacag cagcagcaaa ttacgctttt
    30601 gaaaaattcc cttcaaaaat tgcgtatgtc attacgaatg gacgtgtacg tgaccgcatg
    30661 ggattatctg gaacagggtc aagcggacaa tggacgaatt ggccagatga cgatcacttc
    30721 gatcatattc atttaaacgg ttcgatgggg tctggcgata tattcaatgg aggagttgga
    30781 ggaagtggtg ttgcacgctg gcgttcttat gtgtctaaag cattgaaaat gaatggatta
    30841 ccagctacag cagcttatat aaatgcctgg atgtcccaaa tccaaacgga atctggtggt
    30901 aatgagaaag caatcggcgg caatgatggg ctcgctgaag gcaatgcaac aggattactt
    30961 caaacgaaac ccggaacttt tgcagctaac gcttttcctg gtcacggaaa tattatgaac
    31021 ggatttgata acatgttagc agcaatcaat tatgctaaga aacgttacgg agttgcaggt
    31081 atgttgcaag ttatcgggaa aggacatgga tatgccaatg gcggattgat taccaaagac
    31141 ggactatatc gagcaggaga aggaaataaa ccagaaatgg ttattccatt gacaagaaaa
    31201 acaagagcta ttgaattgat gggtcaagca ttagcttttc tttctggcga tgacaagaaa
    31261 cgctccaaca ctacaaatac agctgataat tcagcagagc ttgtaacttt gattaaacag
    31321 caacaaaaac agcataatga gttaatgatg attcttagag cgattcttgg aaaagattta
    31381 agtctcaagt cttctgacat tgggcaagct gctaatcatt atatgggctc tgatttgaat
    31441 aaacttcgat atacgaatgg aggtgtttga taattgtttt acaaattaca gttcaatcaa
    31501 aatgggaagt tgtttgatcc gcaaagaaaa gaaaaaattg tatgtaaaga aatcaaacgt
    31561 caagcaccag tgtatgaagt gaattacgaa gattttgaag ggacgaacgg taacagagaa
    31621 gctaatgcta gttttcgtcc ttttgaatta gtgctgactt ttgatatctt ttacaaaaat
    31681 aagcatgata aagaactgct attaacagag ttctatgagc taatttttgt tggttatcaa
    31741 tattacattt cctatgattt aagtccaggg aaaagattca aagtaaatcc aacaaatttt
    31801 gaaattactg aggaagagaa cgattattct acgatagaaa taacctttaa tattccatca
    31861 gggagtgctg aatccatcgc aaccacttta acggatttca atctagagga agaatggcaa
    31921 ttctcgcaag ggctagtggc ggaagattac aagtacacgc atcagactag tcacttcatt
    31981 atttacaatg ctggaagctt tgaaattgat ccacgtgaac attatctgcg gatcgcatta
    32041 gaaggagaat cagaaggaaa tgtgacgatt ttcaataaaa caactggcga tcgatttatt
    32101 tactatccat cactttctac gaatctcgga cagacattag tcttggatgg cgtgattcca
    32161 aaattgaacg gtgtaagttg tgggatcaat acgaatcatg gcctaatcaa tttggttgag
    32221 ggtgtcaatg aaatcgagat ccaaaatatt actcgcgtga aatcttcatg ggatttccgt
    32281 ttcttatata agtaggtgat tgagtgactg atttaattat tcgaaattat gaacaaacca
    32341 aagaagaaat ccttgtcggt tatgacaagg gttcttttta tgaaaactgg caacaaaatg
    32401 aaacgtggga gattggcttt accgttacca gcgattcgtt gaatcaagaa gtatttgatt
    32461 tagtcgaata cgagtcttct gttttctaca acggacagga atttgtgatc aaagaaatga
    32521 ctcgcaaagc acttggacaa ttgctgacga aacaagtagt tgcgacacat atctattaca
    32581 ccgttcaaga tggttatcag tacgatacgg taactggcgc gagatctatt agtcagttac
    32641 tcacgcatgt atttagtgca ggtagtcgcg gctttacatg ggaagtcatt gatccaaaca
    32701 agaaattcct tacagtcgaa caagaaaact ttggtaatgc gaattacttg aagctgatca
    32761 atgagattct gtctgactat aatgcagtcg tgattccgaa taataaacat ctaacttttt
    32821 atcctgccag tgagtacggc cagcggacgg aagaacagat tcgttataag tacaatacag
    32881 atgaagtttc atttgatatt gatacgtaca gtctaaaaac acaaatcaag ggttatggaa
    32941 aattaaaaga tggagcgaat actgaggatc ctaaagatag tgactataga tttactccta
    33001 tcacttacac aagtcctgaa tcacagaagt ggggaatcag gatacaagat cctgttaaag
    33061 acgaacggta taccgtatca gggaacatgc tcgagcggtt aaagacagac ttgcaagact
    33121 atccaagtat ttcgggatct gtaacgttga aatggaaaat cagtcccaac aaaggcgatc
    33181 acgtcccatt catttatgaa cctttgaata tcaatacgta cattcaagtg gtaggaatca
    33241 agacgtatcc agcgatacca aataagccac cagaaatcac attgagcaac acaaagaaaa
    33301 caatgacgtc gatactcgct gaaatggcga agaaaggagt gatttgatgg ggttattaaa
    33361 attaatcagt aaccgtatct ctacggaatg gaaagagaaa tttaatcaaa acattgacta
    33421 cctcaatgat cttgaaaaga agctatctga tcaagacaaa tcaacgaaca gtcgtataga
    33481 taatctcgtg cttcattcag gcggtgattc tcctaacgaa gtagtggatg ctcgaataaa
    33541 tgctgaaggt acgatctatc caacgcttta ttcgagatta ttagcattgg ataacctttt
    33601 caatttgaat tatacagaat taaagacaag acaagataat caacaaggtc agctaaatca
    33661 actaaatgtt tctgttggaa ctcttatggg agcatacggc gaaacgctcg atttatatgt
    33721 tgctaaaaca ggaagtgatc aaagtgggga tggtacagaa aaaaatccat ttcttacaat
    33781 tcaagctgct gtaaatcaaa taccgttatt aactagttca cgagtaacta tttggattgg
    33841 cgatggagta taccttgaag atgtcgtgat tcgcaatctc aaagcagtta gcattacttt
    33901 gcgcagtcgc caaagtgtaa cagatgtaac gtctgactta agtgttaaag ttcgctccat
    33961 ttcatttatt agctctttag gttaccaaca agttaatgga atcgagtttg tagatcaggc
    34021 gaacatttca ggacaattaa agtgtgcaat ttattctgaa cagtctagtt atttagctgt
    34081 ctggaattgt cgttttgctg aaactactta tgggaaatca aatcgttgtt tatttgccac
    34141 aggaggttca aaaattgcta caaacaataa ttactaccta aatcaaaact gtattgcaga
    34201 agctagaaat ttagctgaca ttaacattga tctaagtgat caaggaactg gaaatgacta
    34261 tgggataatt gcggataacg gaactgctag aatcaaagtt gttggttcta aggtaaaagc
    34321 gaatagaatt gctgaagtaa gaaatcaagg gaatgtcgtt acaggtaaaa tcattcgcca
    34381 aatcactaat gatgatatta gcgatcgtga caatattaca aacgttaacg gtactattaa
    34441 acgtgaagga gacacagtca caatagcaat caagtatgaa tgcaataatt atccttctga
    34501 tgcttctaac acaagaaatg ttattttggt tcccgctgga tttcaacgtg atcaaagtta
    34561 tcctgcttac catccgctgg ctttatatcg caatgaaacc caacctgctg gcgcaagggc
    34621 aggcttaaca caagctagtc gcgtagtcgc atactcagga aatggatcat cttatatttc
    34681 aggtacatgg gtaacgaatg acccaatacc aattatttaa gaaaggagga tatagatgtt
    34741 taaaattaat gaatcgatta ttgtaattca agcagaagcg atccgtccaa acaatacagg
    34801 cgttgttttt tggtcgcatg atcgaggaac agctaagctt cgaatgaagt tagttcggaa
    34861 aaacggcatc cctcaaagct tacccgaagg gacaactgtt ccgattcgct tgatgttcaa
    34921 atctgcaacg gcagaaggtg gttatggaaa acatgactat ctcgccacca ttgaagatcg
    34981 tgtaaatggt attgtgtcca ttgtgctgga agataatatt ttaggctatg ttggaattgt
    35041 cgaaggtagt gtttatattg atttcccaaa cgaccgctcg ttagatacag ctggtcgttt
    35101 tacttttgac atcaaacgca gtccaatcga tgatagtaca ccagaattgg aagattatta
    35161 tttcaatggt ttcagtcaga ctattgataa aatcgaaaaa attctagctg atggaaagca
    35221 agagattgaa cagaaaatta cgaaatctga aacgcagatt gatgcgaaat taaaagacac
    35281 aaacgacaaa atcacgaaag ccaatcaaga tgtcgcaact atcaatacga atattgataa
    35341 agcaaatgat cgtattggtc aaacgaacca gcaaataagt gatctcggaa agctgaaaaa
    35401 gatgtactcc aacagcatcg atttcgggaa ctatgattat agtgggaatc ctaatttgtt
    35461 aagtaagcta tcatacgact taattgaaaa tcaaaatact tcagctggaa cactttctaa
    35521 aggtgaaaac tcgtttaaat ataataagat atcagctgaa acggaaggtg gagtagagtt
    35581 atattataaa cgaagaggta tagctaactg gttaccctct aataaaacgc ttgtaatgac
    35641 cgttaaactt agagctggag ttgactatag tccagctgac ggaaaaaaaa tactgattag
    35701 atataggttt gttgacaatg gaactggcaa gattgtttta gacttaccta ttaacagtaa
    35761 ttcgataact caggaatgga aagagtttag tattactgga actactccaa catttggccc
    35821 gcaagcatac catccttgga tacaatttag ggctcaagat gggatacttg gggaaataga
    35881 aatgagctat gacatcaaaa tcgaagaagg tccaacagcc acaccatacc agcctaactt
    35941 attggtagaa ccttacaaca tgtgtcgcga atatcctaac gaaaatattg ccgatcctaa
    36001 agttaagttc ccaatcgaat ctggcgacca ccaaatatat caaggttaca cagaagaaga
    36061 gcttatgata ggtcaaacgt atactatcac gcttaaagga acaaaacccg caagtcaaac
    36121 ctttgtagcg tataatcatt ggactgctcg tttaggagaa ctaaagccgg ttgatgggtt
    36181 gacagacgta tggtctctaa cattcacacc aacgaatgtt gtggcgatgc ctaaactttt
    36241 ccgtgtttat cagtatccac gatcaacagt aggcgcatgc caaattgact ggctcaagat
    36301 cgaaaaaggc aacacccgaa cgcccaatat tagtcagttt aaatacttcg gtgaaggatt
    36361 gaaagacagc aacaatccca atgattacag ttgggatgtc acacctgaat atactgaaaa
    36421 agctttaact aatactttaa accttactga tccacaaaca gctctaggaa taaaaaattt
    36481 caaagatggg gcccggatcg acggagtaga tgtggcaaca gttaaaaact taggtagtgt
    36541 gttacagagt ggatctggtt tgactactat tcaatcagat aagcaaagcg catttgatga
    36601 attttcatac gaattcgaac gtattggtga tagggtattc tttcacgcac gagtaaaaac
    36661 aaactcgaca acagccgtag cttcacatgt gaatctacta gaaattcctt taggatttca
    36721 gtttcctgtt gaatggacaa ttgtagggat tccgttgtcg gttgtacagt ggactacgcc
    36781 acaaggagaa atcagcgctt tggtaatcac tgatttaact ggtaaacaag tggttaaatt
    36841 tgcaactaat cgagtaggaa atcattatat ttcaggagaa tggcgaacag ccgatagtta
    36901 tccagaaaca aagtaggagg gaaataaatg aaaaacattt ggaaatacgg gcgtactggc
    36961 ggagagtacg caggaaaagt attggacgac atgcttgtat ccgttcctta cacagatcag
    37021 ccgccacttg aaggattccg tgctgatgga gaaccgctaa cgatcgctga tcagatgttt
    37081 gatcctaaac tgaaccaatg gattgtttta gcgaacacac tggatcacaa cgatttaaac
    37141 aatctcaaag cgatgtacga ggcactggaa cacgagaacg gcgatttaaa acagctcaac
    37201 gccaaactca tgctaagcga tgtagcaatt aaacaggaaa atactgcatt gaaagaaaaa
    37261 gcggatagtt tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa
    37321 gacatttcag aaattaaaga gcaactaaat ccaactttaa agggaggtga gtagtatgtt
    37381 tagttttagc gatgtgaaaa tgatgtatga ttggggctgt tttactgacg atcaagttcg
    37441 actattcgtt ccactatgca ttacagacga agaagcagat aaaatcatta gcaaagaaga
    37501 gagcgcatct taagtgatgc gtttttttgt tggaaagttg gtggaacatg aaagaagaag
    37561 cgctccaaga cgttgtggag agattagtaa gaattgaaac aaaattagac aactacgaat
    37621 cacttagaga aaaggctgat agtgcaaaag atttagcaga taaagcctat tcagtagcac
    37681 taaacaatgc agaagacatc aaggaaatga agaacaataa taaatgggct tggggctata
    37741 tgattggctt aggcattacg atcattggct atttcttgac taaattgtaa aggaggtgag
    37801 aagaaatgat tttaccagat aagtattatc aagtcattaa atggacagtt ttaacagtat
    37861 tgccagctgc atctgcttta gtagccatgt tagggaaagc gtatggatgg aatggaacag
    37921 atatgatagt gttgactatc aatgcagtag caacattttt aggcgttatc actggtgtgt
    37981 cggcttataa tttgaaaaaa taggaggaaa caaatgaaaa agaaaattac tattactgcg
    38041 atgagcctat taacggctct ttttttattg ccaattaatg ggtttgccta tactatcaac
    38101 aatgaattta atttgggcgc aaatgaaggt agctcacaaa tagcaaataa tcagtacatt
    38161 ttactgcatg aaacggctaa tgaaacagca acaggacgca atgaagcgca gtatatgcaa
    38221 cgttcatgga ctagcgctta tactgcttat attgtgggag acggcggaat tgtttatcaa
    38281 gtcggtcaac ctggttatgt acagtacggt gctggttcgt atgctaatgc caacagtcct
    38341 gtgcagattg agttacaaca cacacatgat aaagcaacgt ttgagaaaaa ctacaaggca
    38401 tacgttgaat tggctagaga ttcagcaatg aaatatggta ttccattaac attagacacg
    38461 ccttataacc aaccaggaat caaatcacat ttatgggtaa cacaaaatat ctggggagat
    38521 catactgatc catacggtta tctttctgaa atgggcgtaa gtaaagaaaa attagcatat
    38581 gatttagctc atggatttac cgatgaaaat ccaacaactt ctgaaaacaa gcctgtcatt
    38641 gatccaacac gagctggtgc agctaatcct acactgacag atggaacgaa ttacgcccac
    38701 attgatcagt ttggagaaat cgaaaatgca aatttgcatg tagctggatg gcacattgct
    38761 aattataaat acgagtatat tttcattatg gactacaata ctggaaaaga actagctaga
    38821 gtaagagctg atgggattta tagaccagac gtaaatcaag cttataatac tttaggaaat
    38881 gttggctatc atgtatcttt caatatgcgt gatttcccta ataagaaagt atacgtcatg
    38941 atgcgggcaa cgaatgatcc agagggaaac actaaaggcg gtgcgcaaga tttccatgac
    39001 aaacgttggt atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagca
//
LOCUS       DAPBBG010000022        36567 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPBBG010000022 DAPBBG010000000
VERSION     DAPBBG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.022"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="RD477_001337"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="RD477_001337"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDT7633705.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="RD477_001338"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="RD477_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDT7633706.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="RD477_001339"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="RD477_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDT7633707.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="RD477_001340"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="RD477_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDT7633708.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="RD477_001341"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="RD477_001341"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDT7633709.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="RD477_001342"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="RD477_001342"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDT7633710.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="RD477_001343"
     CDS             complement(8052..8522)
                     /locus_tag="RD477_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDT7633711.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="RD477_001344"
     CDS             complement(8568..8993)
                     /locus_tag="RD477_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDT7633712.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="RD477_001345"
     CDS             complement(9060..9329)
                     /locus_tag="RD477_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDT7633713.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="RD477_001346"
     CDS             complement(9340..10935)
                     /locus_tag="RD477_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7633714.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="RD477_001347"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="RD477_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDT7633715.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="RD477_001348"
     CDS             complement(14488..15048)
                     /locus_tag="RD477_001348"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDT7633716.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="RD477_001349"
     CDS             15401..16375
                     /locus_tag="RD477_001349"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDT7633717.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="RD477_001350"
     CDS             16545..17945
                     /locus_tag="RD477_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDT7633718.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="RD477_001351"
     CDS             complement(18023..18745)
                     /locus_tag="RD477_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633719.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="RD477_001352"
     CDS             complement(18824..19330)
                     /locus_tag="RD477_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7633720.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="RD477_001353"
     CDS             19453..21252
                     /locus_tag="RD477_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDT7633721.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="RD477_001354"
     CDS             complement(21320..22894)
                     /locus_tag="RD477_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7633722.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="RD477_001355"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="RD477_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDT7633723.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="RD477_001356"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="RD477_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDT7633724.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="RD477_001357"
     CDS             complement(24671..25309)
                     /locus_tag="RD477_001357"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDT7633725.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="RD477_001358"
     CDS             complement(25310..25960)
                     /locus_tag="RD477_001358"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDT7633726.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="RD477_001359"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="RD477_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDT7633727.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="RD477_001360"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="RD477_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDT7633728.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="RD477_001361"
     CDS             complement(29103..29843)
                     /locus_tag="RD477_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDT7633729.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="RD477_001362"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="RD477_001362"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDT7633730.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="RD477_001363"
     CDS             complement(31214..32152)
                     /locus_tag="RD477_001363"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDT7633731.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="RD477_001364"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="RD477_001364"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDT7633732.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="RD477_001365"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="RD477_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDT7633733.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="RD477_001366"
     CDS             complement(35312..35617)
                     /locus_tag="RD477_001366"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDT7633734.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="RD477_001367"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="RD477_001367"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDT7633735.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DAPBBG010000023        35781 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPBBG010000023 DAPBBG010000000
VERSION     DAPBBG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.023"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..705)
                     /locus_tag="RD477_001368"
     CDS             complement(<1..705)
                     /locus_tag="RD477_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7633736.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="RD477_001369"
     CDS             1393..1551
                     /locus_tag="RD477_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633737.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2849
                     /locus_tag="RD477_001370"
     CDS             1623..2849
                     /locus_tag="RD477_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7633738.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="RD477_001371"
     CDS             complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="RD477_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDT7633739.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="RD477_001372"
     CDS             complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="RD477_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDT7633740.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4308..4775)
                     /locus_tag="RD477_001373"
     CDS             complement(4308..4775)
                     /locus_tag="RD477_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDT7633741.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4895..6433)
                     /locus_tag="RD477_001374"
     CDS             complement(4895..6433)
                     /locus_tag="RD477_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDT7633742.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6513..7199
                     /locus_tag="RD477_001375"
     CDS             6513..7199
                     /locus_tag="RD477_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDT7633743.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="RD477_001376"
     CDS             complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="RD477_001376"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7633744.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="RD477_001377"
     CDS             complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="RD477_001377"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDT7633745.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8685..9815)
                     /locus_tag="RD477_001378"
     CDS             complement(8685..9815)
                     /locus_tag="RD477_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7633746.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9815..11998)
                     /locus_tag="RD477_001379"
     CDS             complement(9815..11998)
                     /locus_tag="RD477_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDT7633747.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11995..13020)
                     /locus_tag="RD477_001380"
     CDS             complement(11995..13020)
                     /locus_tag="RD477_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7633748.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13065..13892)
                     /locus_tag="RD477_001381"
     CDS             complement(13065..13892)
                     /locus_tag="RD477_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDT7633749.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13889..14956)
                     /locus_tag="RD477_001382"
     CDS             complement(13889..14956)
                     /locus_tag="RD477_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDT7633750.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14982..15818)
                     /locus_tag="RD477_001383"
     CDS             complement(14982..15818)
                     /locus_tag="RD477_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7633751.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15815..16684)
                     /locus_tag="RD477_001384"
     CDS             complement(15815..16684)
                     /locus_tag="RD477_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7633752.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16754..18037)
                     /locus_tag="RD477_001385"
     CDS             complement(16754..18037)
                     /locus_tag="RD477_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDT7633753.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18051..19736)
                     /locus_tag="RD477_001386"
     CDS             complement(18051..19736)
                     /locus_tag="RD477_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDT7633754.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20011..21036
                     /locus_tag="RD477_001387"
     CDS             20011..21036
                     /locus_tag="RD477_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7633755.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="RD477_001388"
     CDS             complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="RD477_001388"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDT7633756.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="RD477_001389"
     CDS             complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="RD477_001389"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDT7633757.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29708..30676
                     /locus_tag="RD477_001390"
     CDS             29708..30676
                     /locus_tag="RD477_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7633758.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30694..31599
                     /locus_tag="RD477_001391"
     CDS             30694..31599
                     /locus_tag="RD477_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633759.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31596..32402
                     /locus_tag="RD477_001392"
     CDS             31596..32402
                     /locus_tag="RD477_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7633760.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32538..33527
                     /locus_tag="RD477_001393"
     CDS             32538..33527
                     /locus_tag="RD477_001393"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDT7633761.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33844..34368)
                     /locus_tag="RD477_001394"
     CDS             complement(33844..34368)
                     /locus_tag="RD477_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDT7633762.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34655..>35781
                     /gene="scm"
                     /locus_tag="RD477_001395"
     CDS             34655..>35781
                     /gene="scm"
                     /locus_tag="RD477_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDT7633763.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSAS"
BASE COUNT        10292 a         7556 c         6624 g        11309 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgcttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctagtgc tttaacacac tcagatattt
     2761 ctacaacgag aatatacgta aatacaccag atgtagtaga taaaactgtt tttgaagcct
     2821 ttcagagagg attgaataaa tgtgattagt atgtgttctt aaatgtgttc ttaaacaaga
     2881 aaaagacact tttcaaacaa ttgaaaagtg ccttgaaaca ttgatatata aataatatta
     2941 acgtttagag aattgagatg ctttacgagc ctttttgaga cctggttttt tacgttcgac
     3001 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     3061 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     3121 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     3181 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     3241 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     3301 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     3361 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     3421 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     3481 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     3541 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     3601 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
     3661 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     3721 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     3781 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     3841 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     3901 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     3961 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     4021 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     4081 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     4141 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     4201 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     4261 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     4321 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     4381 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     4441 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     4501 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     4561 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     4621 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     4681 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     4741 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     4801 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     4861 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     4921 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     4981 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     5041 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     5101 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     5161 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     5221 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     5281 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     5341 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     5401 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     5461 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     5521 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     5581 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     5641 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     5701 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     5761 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     5821 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     5881 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     5941 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     6001 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     6061 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     6121 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     6181 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     6241 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     6301 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     6361 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     6421 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     6481 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     6541 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     6601 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     6661 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     6721 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     6781 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     6841 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     6901 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     6961 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     7021 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     7081 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     7141 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     7201 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     7261 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     7321 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     7381 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     7441 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     7501 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     7561 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     7621 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     7681 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     7741 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     7801 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     7861 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     7921 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     7981 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     8041 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     8101 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     8161 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     8221 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     8281 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     8341 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     8401 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     8461 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     8521 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     8581 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     8641 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     8701 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     8761 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     8821 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     8881 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     8941 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     9001 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     9061 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     9121 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     9181 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     9241 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     9301 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     9361 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     9421 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     9481 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     9541 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     9601 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     9661 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     9721 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     9781 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     9841 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     9901 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     9961 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    10021 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    10081 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    10141 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    10201 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    10261 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    10321 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    10381 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    10441 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    10501 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    10561 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    10621 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    10681 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    10741 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    10801 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    10861 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    10921 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    10981 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    11041 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    11101 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    11161 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    11221 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    11281 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    11341 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    11401 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    11461 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    11521 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    11581 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    11641 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    11701 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    11761 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    11821 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    11881 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    11941 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    12001 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    12061 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    12121 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    12181 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    12241 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    12301 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    12361 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    12421 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    12481 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    12541 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    12601 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    12661 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    12721 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    12781 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    12841 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    12901 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    12961 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    13021 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    13081 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    13141 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    13201 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    13261 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    13321 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    13381 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    13441 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    13501 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    13561 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    13621 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    13681 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    13741 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    13801 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    13861 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    13921 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    13981 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    14041 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    14101 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    14161 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    14221 ctattaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    14281 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    14341 tgtaatcggc accgtgtttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    14401 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    14461 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    14521 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    14581 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    14641 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    14701 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    14761 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    14821 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    14881 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    14941 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    15001 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    15061 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    15121 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    15181 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    15241 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    15301 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    15361 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    15421 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    15481 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    15541 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    15601 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    15661 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    15721 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    15781 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    15841 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    15901 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    15961 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    16021 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    16081 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    16141 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    16201 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    16261 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    16321 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    16381 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    16441 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    16501 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    16561 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    16621 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    16681 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    16741 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    16801 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    16861 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    16921 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    16981 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    17041 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    17101 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    17161 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    17221 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    17281 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    17341 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    17401 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    17461 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    17521 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    17581 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    17641 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    17701 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    17761 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    17821 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    17881 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    17941 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    18001 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    18061 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    18121 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    18181 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    18241 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    18301 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    18361 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    18421 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    18481 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    18541 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    18601 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    18661 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    18721 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    18781 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    18841 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    18901 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    18961 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    19021 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    19081 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    19141 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    19201 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    19261 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    19321 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    19381 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    19441 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    19501 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    19561 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    19621 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    19681 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    19741 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    19801 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    19861 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    19921 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    19981 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    20041 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    20101 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    20161 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    20221 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    20281 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    20341 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    20401 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    20461 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    20521 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    20581 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    20641 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    20701 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    20761 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    20821 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    20881 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    20941 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    21001 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    21061 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    21121 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    21181 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    21241 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    21301 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    21361 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    21421 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    21481 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    21541 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    21601 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    21661 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    21721 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    21781 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    21841 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    21901 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    21961 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    22021 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    22081 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    22141 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    22201 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    22261 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    22321 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    22381 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    22441 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    22501 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    22561 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    22621 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    22681 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    22741 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    22801 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    22861 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    22921 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    22981 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    23041 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    23101 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    23161 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    23221 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    23281 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    23341 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    23401 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    23461 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    23521 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    23581 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    23641 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    23701 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    23761 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    23821 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    23881 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    23941 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    24001 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    24061 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    24121 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    24181 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    24241 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    24301 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    24361 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    24421 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    24481 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    24541 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    24601 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    24661 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    24721 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    24781 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    24841 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    24901 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    24961 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    25021 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    25081 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    25141 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    25201 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    25261 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    25321 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    25381 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    25441 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    25501 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    25561 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    25621 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    25681 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    25741 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    25801 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    25861 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    25921 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    25981 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    26041 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    26101 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    26161 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    26221 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    26281 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    26341 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    26401 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    26461 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    26521 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    26581 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    26641 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    26701 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    26761 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    26821 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    26881 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    26941 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    27001 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    27061 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    27121 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    27181 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    27241 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    27301 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    27361 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    27421 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    27481 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    27541 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    27601 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    27661 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    27721 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    27781 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    27841 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    27901 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    27961 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    28021 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    28081 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    28141 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    28201 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    28261 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    28321 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    28381 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    28441 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    28501 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    28561 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    28621 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    28681 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    28741 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    28801 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    28861 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    28921 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    28981 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    29041 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    29101 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    29161 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    29221 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    29281 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    29341 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    29401 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    29461 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    29521 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    29581 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    29641 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    29701 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    29761 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    29821 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    29881 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    29941 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    30001 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    30061 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    30121 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    30181 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    30241 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    30301 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    30361 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    30421 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    30481 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    30541 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    30601 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    30661 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    30721 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    30781 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    30841 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    30901 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    30961 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    31021 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    31081 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    31141 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    31201 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    31261 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    31321 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    31381 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    31441 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    31501 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    31561 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    31621 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    31681 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    31741 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    31801 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    31861 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    31921 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    31981 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    32041 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    32101 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    32161 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    32221 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    32281 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    32341 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    32401 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    32461 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    32521 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    32581 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    32641 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    32701 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    32761 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    32821 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    32881 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    32941 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    33001 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    33061 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    33121 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    33181 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    33241 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    33301 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    33361 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    33421 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    33481 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    33541 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    33601 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    33661 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    33721 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    33781 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    33841 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    33901 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    33961 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    34021 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    34081 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    34141 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    34201 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    34261 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    34321 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    34381 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    34441 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    34501 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    34561 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    34621 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    34681 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    34741 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    34801 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    34861 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    34921 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    34981 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    35041 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    35101 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    35161 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    35221 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    35281 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    35341 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    35401 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    35461 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    35521 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    35581 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    35641 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    35701 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga
    35761 tagcacggat acatcagctt c
//
LOCUS       DAPBBG010000024        35450 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPBBG010000024 DAPBBG010000000
VERSION     DAPBBG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.024"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="RD477_001396"
     CDS             complement(99..1229)
                     /locus_tag="RD477_001396"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDT7633764.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="RD477_001397"
     CDS             complement(1878..3080)
                     /locus_tag="RD477_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7633765.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="RD477_001398"
     CDS             complement(3140..3343)
                     /locus_tag="RD477_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDT7633766.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="RD477_001399"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="RD477_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="RD477_001400"
     CDS             4880..5692
                     /locus_tag="RD477_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633767.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="RD477_001401"
     CDS             complement(6716..6868)
                     /locus_tag="RD477_001401"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDT7633768.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="RD477_001402"
     CDS             complement(7059..7937)
                     /locus_tag="RD477_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7633769.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="RD477_001403"
     CDS             complement(7976..9094)
                     /locus_tag="RD477_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDT7633770.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="RD477_001404"
     CDS             complement(9091..11190)
                     /locus_tag="RD477_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDT7633771.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="RD477_001405"
     CDS             complement(11241..12062)
                     /locus_tag="RD477_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7633772.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="RD477_001406"
     CDS             complement(12059..12868)
                     /locus_tag="RD477_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7633773.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="RD477_001407"
     CDS             complement(12889..13383)
                     /locus_tag="RD477_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7633774.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="RD477_001408"
     CDS             complement(13395..13883)
                     /locus_tag="RD477_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7633775.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="RD477_001409"
     CDS             complement(13960..16656)
                     /locus_tag="RD477_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7633776.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="RD477_001410"
     CDS             complement(17084..18157)
                     /locus_tag="RD477_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7633777.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="RD477_001411"
     CDS             complement(18154..18981)
                     /locus_tag="RD477_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633778.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="RD477_001412"
     CDS             complement(18982..19788)
                     /locus_tag="RD477_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633779.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="RD477_001413"
     CDS             complement(19800..20885)
                     /locus_tag="RD477_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633780.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="RD477_001414"
     CDS             complement(20902..21444)
                     /locus_tag="RD477_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7633781.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="RD477_001415"
     CDS             21702..22691
                     /locus_tag="RD477_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDT7633782.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="RD477_001416"
     CDS             22688..23425
                     /locus_tag="RD477_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDT7633783.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="RD477_001417"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="RD477_001417"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDT7633784.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="RD477_001418"
     CDS             23989..24621
                     /locus_tag="RD477_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7633785.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="RD477_001419"
     CDS             24706..25353
                     /locus_tag="RD477_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7633786.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="RD477_001420"
     CDS             25358..26341
                     /locus_tag="RD477_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDT7633787.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="RD477_001421"
     CDS             complement(26744..28258)
                     /locus_tag="RD477_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDT7633788.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="RD477_001422"
     CDS             28396..29202
                     /locus_tag="RD477_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633789.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="RD477_001423"
     CDS             complement(29377..29946)
                     /locus_tag="RD477_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7633790.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="RD477_001424"
     CDS             complement(30029..32089)
                     /locus_tag="RD477_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDT7633791.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="RD477_001425"
     CDS             complement(32366..33844)
                     /locus_tag="RD477_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633792.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34142..35077)
                     /gene="murB"
                     /locus_tag="RD477_001426"
     CDS             complement(34142..35077)
                     /gene="murB"
                     /locus_tag="RD477_001426"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDT7633793.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35150..>35450)
                     /locus_tag="RD477_001427"
     CDS             complement(35150..>35450)
                     /locus_tag="RD477_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7633794.1"
                     /translation="MDLASGANIEKYQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGD
                     WTNTLVKRGFEPMVEAFFSQIRLGKSETELLKQKEVVRSHELCEEVLKDHYRHQL"
BASE COUNT        10590 a         7398 c         5962 g        11500 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
    34141 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
    34201 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
    34261 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
    34321 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
    34381 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
    34441 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
    34501 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
    34561 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
    34621 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
    34681 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
    34741 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
    34801 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
    34861 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
    34921 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
    34981 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
    35041 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
    35101 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
    35161 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
    35221 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
    35281 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
    35341 ttccatcagc atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
    35401 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc
//
LOCUS       DAPBBG010000025        34918 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPBBG010000025 DAPBBG010000000
VERSION     DAPBBG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.025"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="RD477_001428"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="RD477_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDT7633795.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="RD477_001429"
     CDS             complement(2017..2919)
                     /locus_tag="RD477_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7633796.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="RD477_001430"
     CDS             complement(2916..3608)
                     /locus_tag="RD477_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDT7633797.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="RD477_001431"
     CDS             complement(3651..4292)
                     /locus_tag="RD477_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7633798.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="RD477_001432"
     CDS             complement(4330..4644)
                     /locus_tag="RD477_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633799.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5984)
                     /locus_tag="RD477_001433"
                     /pseudo
     CDS             complement(4632..5984)
                     /locus_tag="RD477_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5981..6913)
                     /locus_tag="RD477_001434"
     CDS             complement(5981..6913)
                     /locus_tag="RD477_001434"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDT7633800.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="RD477_001435"
     CDS             complement(6910..7686)
                     /locus_tag="RD477_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDT7633801.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="RD477_001436"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="RD477_001436"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDT7633802.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /gene="carA"
                     /locus_tag="RD477_001437"
     CDS             complement(10930..12006)
                     /gene="carA"
                     /locus_tag="RD477_001437"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDT7633803.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="RD477_001438"
     CDS             complement(12011..13291)
                     /locus_tag="RD477_001438"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDT7633804.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="RD477_001439"
     CDS             complement(13310..14236)
                     /locus_tag="RD477_001439"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDT7633805.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="RD477_001440"
     CDS             complement(14249..15532)
                     /locus_tag="RD477_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDT7633806.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="RD477_001441"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="RD477_001441"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDT7633807.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="RD477_001442"
     CDS             complement(16320..17231)
                     /locus_tag="RD477_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7633808.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /locus_tag="RD477_001443"
     CDS             complement(17228..17692)
                     /locus_tag="RD477_001443"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDT7633809.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="RD477_001444"
     CDS             complement(17697..18395)
                     /locus_tag="RD477_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7633810.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="RD477_001445"
     CDS             complement(18382..18768)
                     /locus_tag="RD477_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633811.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="RD477_001446"
     CDS             complement(18915..20585)
                     /locus_tag="RD477_001446"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDT7633812.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="RD477_001447"
     CDS             20768..21001
                     /locus_tag="RD477_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDT7633813.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="RD477_001448"
     CDS             complement(21100..21531)
                     /locus_tag="RD477_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDT7633814.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="RD477_001449"
     CDS             21610..22023
                     /locus_tag="RD477_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDT7633815.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="RD477_001450"
     CDS             complement(22061..23209)
                     /locus_tag="RD477_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7633816.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="RD477_001451"
     CDS             complement(23331..23927)
                     /locus_tag="RD477_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7633817.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="RD477_001452"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="RD477_001452"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDT7633818.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="RD477_001453"
     CDS             27157..27588
                     /locus_tag="RD477_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7633819.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="RD477_001454"
     CDS             complement(27633..28982)
                     /locus_tag="RD477_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7633820.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="RD477_001455"
     CDS             complement(29052..30215)
                     /locus_tag="RD477_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDT7633821.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="RD477_001456"
     CDS             30344..30667
                     /locus_tag="RD477_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633822.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="RD477_001457"
     CDS             complement(30850..32199)
                     /locus_tag="RD477_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7633823.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="RD477_001458"
     CDS             complement(32268..33221)
                     /locus_tag="RD477_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDT7633824.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="RD477_001459"
     CDS             complement(33323..34513)
                     /locus_tag="RD477_001459"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDT7633825.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9940 a         7628 c         6115 g        11235 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaacgata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaatggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgca ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaagg
//
LOCUS       DAPBBG010000026        34216 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPBBG010000026 DAPBBG010000000
VERSION     DAPBBG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.026"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1350
                     /locus_tag="RD477_001460"
     CDS             <1..1350
                     /locus_tag="RD477_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7633826.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="RD477_001461"
     CDS             1484..2827
                     /locus_tag="RD477_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7633827.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="RD477_001462"
     CDS             2939..4390
                     /locus_tag="RD477_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDT7633828.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="RD477_001463"
     CDS             4739..5371
                     /locus_tag="RD477_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDT7633829.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5959..7398
                     /locus_tag="RD477_001464"
     CDS             5959..7398
                     /locus_tag="RD477_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7633830.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7693..7911
                     /locus_tag="RD477_001465"
                     /pseudo
     CDS             7693..7911
                     /locus_tag="RD477_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8160..8375
                     /locus_tag="RD477_001466"
     CDS             8160..8375
                     /locus_tag="RD477_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633831.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8592..8848)
                     /locus_tag="RD477_001467"
                     /pseudo
     CDS             complement(8592..8848)
                     /locus_tag="RD477_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9035..9946)
                     /locus_tag="RD477_001468"
     CDS             complement(9035..9946)
                     /locus_tag="RD477_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7633832.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9965..10969)
                     /locus_tag="RD477_001469"
     CDS             complement(9965..10969)
                     /locus_tag="RD477_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDT7633833.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10966..13089)
                     /locus_tag="RD477_001470"
     CDS             complement(10966..13089)
                     /locus_tag="RD477_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7633834.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13094..15541)
                     /locus_tag="RD477_001471"
     CDS             complement(13094..15541)
                     /locus_tag="RD477_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7633835.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15528..15917)
                     /locus_tag="RD477_001472"
     CDS             complement(15528..15917)
                     /locus_tag="RD477_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7633836.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15977..16480)
                     /locus_tag="RD477_001473"
     CDS             complement(15977..16480)
                     /locus_tag="RD477_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDT7633837.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16493..16714)
                     /locus_tag="RD477_001474"
     CDS             complement(16493..16714)
                     /locus_tag="RD477_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633838.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16719..16856)
                     /locus_tag="RD477_001475"
     CDS             complement(16719..16856)
                     /locus_tag="RD477_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDT7633839.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16853..18037)
                     /locus_tag="RD477_001476"
     CDS             complement(16853..18037)
                     /locus_tag="RD477_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7633840.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18296..18586)
                     /locus_tag="RD477_001477"
     CDS             complement(18296..18586)
                     /locus_tag="RD477_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633841.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18576..19718)
                     /gene="dcm"
                     /locus_tag="RD477_001478"
                     /pseudo
     CDS             complement(18576..19718)
                     /gene="dcm"
                     /locus_tag="RD477_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19794..19958)
                     /locus_tag="RD477_001479"
     CDS             complement(19794..19958)
                     /locus_tag="RD477_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633842.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19951..20625)
                     /locus_tag="RD477_001480"
     CDS             complement(19951..20625)
                     /locus_tag="RD477_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7633843.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20703..22043)
                     /locus_tag="RD477_001481"
     CDS             complement(20703..22043)
                     /locus_tag="RD477_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDT7633844.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22126..22461)
                     /locus_tag="RD477_001482"
     CDS             complement(22126..22461)
                     /locus_tag="RD477_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDT7633845.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22471..22845)
                     /locus_tag="RD477_001483"
     CDS             complement(22471..22845)
                     /locus_tag="RD477_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7633846.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22858..23172)
                     /locus_tag="RD477_001484"
     CDS             complement(22858..23172)
                     /locus_tag="RD477_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7633847.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23285..26512)
                     /gene="fss3"
                     /locus_tag="RD477_001485"
     CDS             complement(23285..26512)
                     /gene="fss3"
                     /locus_tag="RD477_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDT7633848.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(26929..28095)
                     /locus_tag="RD477_001486"
     CDS             complement(26929..28095)
                     /locus_tag="RD477_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7633849.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(28150..28752)
                     /locus_tag="RD477_001487"
     CDS             complement(28150..28752)
                     /locus_tag="RD477_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7633850.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            28875..29075
                     /locus_tag="RD477_001488"
     CDS             28875..29075
                     /locus_tag="RD477_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633851.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            29087..29413
                     /locus_tag="RD477_001489"
     CDS             29087..29413
                     /locus_tag="RD477_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633852.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            29566..30618
                     /locus_tag="RD477_001490"
     CDS             29566..30618
                     /locus_tag="RD477_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDT7633853.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            30694..30912
                     /locus_tag="RD477_001491"
     CDS             30694..30912
                     /locus_tag="RD477_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633854.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            30899..33577
                     /locus_tag="RD477_001492"
     CDS             30899..33577
                     /locus_tag="RD477_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633855.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            33568..33765
                     /locus_tag="RD477_001493"
     CDS             33568..33765
                     /locus_tag="RD477_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633856.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            33769..>34216
                     /gene="mobC"
                     /locus_tag="RD477_001494"
     CDS             33769..>34216
                     /gene="mobC"
                     /locus_tag="RD477_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDT7633857.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFK"
BASE COUNT        10910 a         6406 c         6128 g        10772 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttggggggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     5641 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     5701 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     5761 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     5821 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     5881 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     5941 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     6001 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     6061 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     6121 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     6181 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     6241 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     6301 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     6361 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     6421 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     6481 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     6541 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     6601 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     6661 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     6721 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     6781 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     6841 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     6901 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     6961 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     7021 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     7081 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     7141 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     7201 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     7261 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     7321 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     7381 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     7441 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     7501 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     7561 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     7621 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     7681 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     7741 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     7801 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     7861 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     7921 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     7981 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     8041 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     8101 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     8161 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     8221 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     8281 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     8341 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     8401 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     8461 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     8521 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     8581 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     8641 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     8701 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     8761 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     8821 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     8881 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     8941 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     9001 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     9061 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     9121 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     9181 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     9241 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     9301 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     9361 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     9421 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     9481 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     9541 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     9601 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     9661 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     9721 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     9781 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     9841 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     9901 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     9961 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
    10021 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
    10081 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
    10141 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
    10201 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
    10261 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
    10321 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
    10381 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
    10441 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
    10501 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
    10561 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
    10621 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
    10681 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
    10741 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
    10801 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
    10861 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
    10921 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
    10981 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
    11041 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
    11101 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
    11161 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
    11221 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
    11281 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
    11341 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
    11401 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
    11461 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
    11521 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
    11581 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
    11641 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
    11701 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
    11761 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
    11821 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
    11881 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
    11941 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
    12001 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
    12061 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
    12121 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
    12181 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
    12241 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
    12301 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
    12361 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
    12421 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
    12481 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
    12541 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
    12601 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
    12661 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
    12721 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
    12781 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
    12841 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
    12901 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    12961 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    13021 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    13081 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    13141 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    13201 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    13261 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    13321 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    13381 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    13441 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    13501 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    13561 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    13621 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    13681 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    13741 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    13801 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    13861 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    13921 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    13981 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    14041 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    14101 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    14161 taactgaacc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    14221 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    14281 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    14341 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    14401 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    14461 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    14521 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    14581 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    14641 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    14701 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    14761 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    14821 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    14881 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    14941 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    15001 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    15061 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    15121 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    15181 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    15241 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    15301 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    15361 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    15421 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    15481 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    15541 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    15601 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    15661 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    15721 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    15781 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    15841 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    15901 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    15961 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    16021 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    16081 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    16141 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    16201 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    16261 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    16321 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaaccca attcgttctt
    16381 tcacttcatc aaaatcgaca ggtggggtaa accacgcccc caccaattca ccttcgttat
    16441 atttcccgag gttcgcaata taaacacgca tctgttccat cgtgcttccc ccttacgcac
    16501 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    16561 atccgaccgc aatcaaagca atcaccaaaa agccaatcag tttggaaaac tcccgcttaa
    16621 atcctaaaaa caaaccaatg accacaattg ccagtaagac taaggattgg gcattggtta
    16681 aaaaccattg atataaattt tgtccgaaat tcatcttgtc actttccttt cttttccatt
    16741 gctcgatccg ctgctgcact tgcattcatc aagcacatca cagtaacacc aagcatactg
    16801 ccaccaaata ccaaaaaaat atcttttaca attcccatga cgattcctct tttcatataa
    16861 tcaaattttc aattgccata ctttgttgct ccaaaatctt tttatgtcgg tctgataagc
    16921 gtgcattctg taccatatcc tggatatagg ttgtccgatt caatcgatcc aactccttag
    16981 ccattttcca cgtaggggcg acttgtcggc ctagccaatt caaggttcgt tcaatggtat
    17041 acggttttgg ctcggaagtt aattgaatct ctttccgatt ccgtccaata aaacgccccc
    17101 attgttcatt caagggccag ttggttcgcc ttttaccttc tactttatcc gcaaatcgca
    17161 agtaacgatt gataatggaa aacgtggtgc tctcaatgct acgacctttt aacaaatctt
    17221 gaatcgcatg aaaagcacgg tcatttttca gtcgaatctc aaaacgattt ttcgtctcag
    17281 tttggtctaa tggaatccct aacttgatat attgttcata gtctttttca tacaaacaga
    17341 aataaacctc actcttgaga ctgccgatat ataaggtatt gcccatgccg tccttttcat
    17401 cagcctttaa aagttccccg gaacgataac ttttaaaggt acgaaacaag gagatacatt
    17461 cttccttctg acattttttc gttagctcag gtatatccaa tagccccact cgatcattga
    17521 ttgccaagtc caagcgtttc atcacgccac cggcctttag acaatcctcg aaaaagtcgt
    17581 accagctacg cttttgagcg agtaaaaaag tttcaaattg tcggcaacct cgccccttta
    17641 attctaaaag cacaccttta tcttcttcat gagacaacat caccacaata tcacccatga
    17701 catactgttc ttgataggag taaaaggcat aatcttcatg gagcatatac tcaaatttca
    17761 aatggagaat ttcttgaata atttttcgtg catccgttgt gggaaagcga atccttacat
    17821 agtcaaacag taacgtcaaa ggcaaatcgg gattaaagct ttccagttgg tcaaaaattt
    17881 tcctcatggt ttttatagat gcggttacgc caccattttc tatctgattt aagtacggcc
    17941 gactaagtcc acaagccgta gccaatttat tttgtgaaac gccgtattcg ttgcgtcggt
    18001 cttttaatag ccgataatct aaattccgtt gtgccaagtt ctcacctcca aattcaaggt
    18061 gttaactttt tcaaatgagt taacacctat acaaattact tactatcttt gatacaacaa
    18121 gcattcttaa tggttgctta cttatttttt gtgtttttgt acccccctgt tagatacggg
    18181 ggtgagacat catggtcggc taacgccgtc catgaacagg cacagcacca cggaggattt
    18241 ctgcttcgcg aatcctccgc ggtcagcgcc taacagtcag ttgtgttggt ttaggttact
    18301 catctgtaat accatttact tccaactctg ctttcatttc taacaagtat tgacgaatat
    18361 ctggttcttt tgcttggtcc gcaatccgct caaattctgc aattacttga tttatttcaa
    18421 ttcgagcatc cgtctcctca ttcattaaat atggatcgcc agcgactttg acaccattct
    18481 tgcgaatatc aaattttaac cgtcgcaatt gttcttctaa ttctgggtct gactgaatct
    18541 ttccaatcac ccaataaatt tctccaatca actcatcata aaccatgtac atccaactct
    18601 tttaatcttc tggcaatatc aaaaatgacc ggaactgtta cagagtttcc ggcttgctta
    18661 tacaactgac tatctgaatt gacttgagaa tctcgatcaa aagcccaatc gggaaagccc
    18721 tgaagtcgcc aacactctct aggtgttagc ttccgaattt gatactgatc cgtgacgatc
    18781 gcttgttggt ctcctgttag taaggtatga acactctgtt ttcccagacg acctcggcga
    18841 cttgtggaat taggaaaagc cagattcaca ctgtcttctg gcttcacctc ttgatattct
    18901 gtttcatttt ttcgaatcat gatttttgta ccaccttgtt tcgttgacaa ggtgggggcg
    18961 gttccagtaa cttcataaac tcgattacct tgttcaaact ttcccggtaa tagtcctgct
    19021 acagagattc catcccttgc tggcagtgcg attttgattc cttcatcacc tgttaaggtt
    19081 ggcgctacgc catctgaacc atagacttgt ccacccattc ctcgattgga aggattgata
    19141 tttaccaaat ttctgatgtt ttgatcagat gttgaagacg gtccaatgat aggaaatact
    19201 tgttcggtac atgcgtctcg agaatgtccg ataaggaata tccgctcccg gctttgggga
    19261 atgtaggcct ttgagttaag cacctaccac tccacgtcat accccaatcc atccagcgtt
    19321 cggaggatcg tctcgaacgt agcccctcct tcgtgattga gcaatcctcg gacgttctca
    19381 aggaataaaa gcttaggtcg gagaagagtg gtgaaccgag cgatttcaaa gaataaagta
    19441 cctcgagtat cggaaaatct ttgccgcttt cccgcaattg aaaaagcttg gcacggaaat
    19501 ccgccgcaaa ggatgtccac tggtccgagg gattgaataa actcatctga tacacttgtg
    19561 atgtcatgca tttccacctc cttacttgta tcatgaattg ctttataact acgtctagca
    19621 aatttatcaa tttcacaaaa accgatacaa tgatggctcg cctgttccat acctaaacga
    19681 aagccaccaa taccggcgaa taaatctaaa aatctcatac attacctcat tcattgcaaa
    19741 gaaaaaagcc aagaacattt tgctcttggc tcatacatta ttaatttgtc ctactatttt
    19801 ttatttaatc caactaattt ccgttgtttt tcattggcct gatcggctac atatttaatc
    19861 accgttttga actcttttgc agtagcattt tccgataaat ccgcaatgct cttattgtat
    19921 ttctttacaa ttttcgttac ttgttctttc tcagtcatgt aattgaacct ccaaatcttt
    19981 cctactatac cctagtggta aaaataattc tagattatcc gtagcataat gaacttcgga
    20041 aatattattc gtataatctt ttattatgat atgaagcaat acatctggat gtttactctt
    20101 tacttttgca atgttctctc ttgatttgaa aaaagcgtta ataaatgcct ttttcggcac
    20161 atgacgtccc tctgcaattt cacgttcttg tgtaaatttc caagcgattt tagggtcttg
    20221 ataaacatag aaaatcgtca ctcgaaaatg ttttttcaac gcccgcgaga tatttttttc
    20281 agcacttaat attgcaaaag ttgcatccag aataaaagaa tagccatgat ctaagacata
    20341 tctaaatgcc tgctctacca accaagaaga tgctttttga aagagactag agttttttcc
    20401 attataatca ggaaactggg tgcgaaagta atccgcatca ataatcacat ggttatcata
    20461 attctcagct aatccaatgg caacctctga ttttccagcc ccaggactcc ctgccataaa
    20521 aactgcgtct cgtccaactt cgggggattt ccctttgata atataagaaa taaacttatc
    20581 cttatttgct tttgcatact ctaaataaga atcattactt tccatattaa ttatcccctt
    20641 tcttcctact atataaaagt attatacgtt acttgaagta taaagtcagt attgaagatc
    20701 actcaatcat tcatcttttc taatttacta attgaatcta agaaatcata cccttttggt
    20761 acaagtggcg tataaaactc actaatcaca ctgccacctg tatccacata acctcgacct
    20821 ttgatgtgct tcataaagaa gtttttttcg acctccccaa acatcatgga atatccgagt
    20881 tcactcatac gacccaaagc cacgcgaaag ttgaattgat cgcggattcc gtctcctaaa
    20941 tatttcgcat ccggcctttg gcaagccaaa atcagaaaga aaccagattg acgacccaac
    21001 atcacgattt gtttgagctt attcaaaatc accgcacttt ctttcgtcgt taacatctcc
    21061 atataagcca cgtattcatc aaagattaaa aagttgggtg gaagtccaag ataggcataa
    21121 ttctctcctg ttttgtagtt agacatttct ttcattgcct tgctacgagc catcatacgt
    21181 tcatagaaat cttccacaca agcagaaatt tcttcctttt gtgaataaac atgaggcatc
    21241 accgtaccta aatcagctaa atctgcattt ttgggatcga ggataaacaa ttctgcatct
    21301 gacttcaata gtgcttcgat aatggtgagg aggaaatagg tcttccctcc acccgtgcca
    21361 ccagcgatta acatatgggg taaagaatca taagcccata cttgattttt catcaatcgc
    21421 agagtcccat tttctgccac tacttcgtct atcccaattc gattggcgat catatcatac
    21481 agtaaagtat actccacata ggagtctttg agttcctttt ctactaactc acaatacagt
    21541 ccgctttcta acttcttttc caattttaaa agctgatctt ggtatttccc aagtgaaatt
    21601 tgaacgcgaa tagacagtaa tccatctttc attcgataat agattttggg gaagtaactg
    21661 atagtctctc tggttcggct actattcaaa tctttgaaaa agccttcact ttgagtttgt
    21721 ttcacttcat accaatggtt ctctagaatc atccgagcta gcttttgtcg atgccacaag
    21781 cttcgccaat gatcaagaaa aaagtgaata taaagcaata tcatagctcc tgaaaaagcg
    21841 agacttccaa gcagacatac taaatctttt atagaccaaa cgatttcaat agtagaaagt
    21901 gatagcttag aaatatcaaa ttgtctaaac tcttgaaaat aaaagacgct catccaaccg
    21961 atgaacagag cgagtagcca gccaagaaca aagtgataaa ccaagtgctg atctcctgcg
    22021 cgtatgcgat gtcctttgta cattttgatg gtttccacct ccaatcgaaa aagcccaata
    22081 ggatttcatt aggcaatttc aaaaagttat gctagtcatt tactgctacg aggaggggag
    22141 ttgtttcgtt tcaaccaatt gatttaaaaa gccttcaata gcatccggca tctccacacc
    22201 aatttcttcc aaaatggatt ctgcctgccg acataagttt tcatttactt cgacaactat
    22261 aaagaaagac tcttcttctc ctaaccagtc tagtacatca gagtcttctt cactgtcgct
    22321 attttctcca agtaaacctt gatgtataat cagaaacatt ttttcatcag aggttaatgc
    22381 tttaacgagt tgttcctcag tcagctcgcc ttttccatat tgatcgagac aatctaaaat
    22441 atgttcaaca ttaatttcca taatcttcac ctatttcttt ctttcatttt ggttcgtagg
    22501 atttccttga ttcggctgcc gcttcaagac taaatcatcc gccttaatga accaattaac
    22561 ggtagttcct tctcgaaaaa cataattggc aacggtatcc acgattggat tgaccaactc
    22621 tacctctgca tcataagcaa actcgcgtaa gggtacatta gctgggatac taacttgaat
    22681 cattcgccct tgtgcagagg attttaaatt ataggttcgt tcttttacct cattaccaat
    22741 tgttccatcc tcattttgct tccgtacttc tcggcgtaat gcggaaaaac gcaacttccc
    22801 aaatgttaaa cctgaatcta cgacaatacc ttctgttaat ctcatcttct tttcctccta
    22861 tgctttcact aaatcatcaa catacaacac gtaatcagta aacccacgat tctcaatctc
    22921 atagcctttg gcaaccaaat ggggattcac ggcttttagc ggttggtctt gatcaaaatc
    22981 cttttcaccg gcttccgcag ctactaccac ctcaatatca tcggcccgtt gcttactgga
    23041 atacaaatga taactgcgac caatcaccgt agtattccgt ccatatcctt ctgttataat
    23101 ttctccttca ccggcataac ttaactttcc aaaggtttca gccatatctg gcacgacaaa
    23161 tttcaattcc atcttctttt cctcttttca ttgttagttt tggatacaaa aatagcgcga
    23221 ctactttttt aggaggcggt cgcgcacgtt ggttcttagg atttacttgt atcatctcat
    23281 caccttagtt ccggcgattc cagaaataat agcccgccgt cgcaaaaatc aaagtaaaac
    23341 caatgaacag taaaacattg gaatttttct ccccggtttg agggaaggtc ttcactgtgg
    23401 gactagattc ttgagagcca cttggaacag ttggtgtttc cggttgtttc ggcgtactta
    23461 gtatggttgg aacttctttt ggtgttaagg tttgagattt ttctttcaaa tcttcattgt
    23521 gttttccatt gatattccca tctttatcgt aattgatttc cgcaaaagta aacttggttc
    23581 cttctggata ttttccggta tctacttttt tcgcaagcac agttttggtg aattctttat
    23641 catttacttc ataatcaatc ttgccagatt tccaaatttc tttgtttgta ccatccggta
    23701 ataaagcata cagaattgtt tcgaaagctt cttttgagcc atccagtaca tcatgagtaa
    23761 tcgacacatc atcaaacata tccaccacat caccatgagt aaaggtctga gaaccgtttt
    23821 ctaggtgggc ctttgtttgg atagaaacat ggtgttccac tgtacaactg acagtttggg
    23881 cttggttatt caagctaaca tccttcgcta ctggcttatc gcctgcttca aaagcttcct
    23941 tattttcata cacatagttg aataagacaa tagttttatt taaaacttgt tcggcggtta
    24001 atttatgctg aattttccaa gttcctgttt tctctttgtt cgccaacgtt gcgcttgttt
    24061 ctgcaatcac cactggtttg gctttttcat ctttttcttg ggcagctttt gtcgcatcca
    24121 catcaatggc ttgcgctacc acataccagt cttctttgat ttcatgtaga ttgtaactca
    24181 atttatcaac tagctcggta gaatccagag aagttaattc tttgtttccg tctttccaag
    24241 tcgcaagaga agttaaacta tcttcttcct caggcaaatc atagagcatt aaacggttca
    24301 aactaacaga aaatgctttg ttcgtcagct tctcgtaagg aacggtcacc gttttaattg
    24361 gttgtttttg gtcttgttcg gtaatggtaa agacatactc actcttcaca aagtcttctt
    24421 tgttttcctt aaaagtagaa cggatttcta atggcgtaat cttttgaaaa ccttctgggg
    24481 cttctacttc ttcaagtaaa taatccccat aaggaagatt ttcaaacttg ccataaccat
    24541 caaaccctaa ttgctcgtta taagctgtgg tcgcttcatc ttcggcacct gtgatttcat
    24601 tggtcccttc caatggcgac actttaaagg tcagatcatt gaagccagtt tcggtagtgc
    24661 ccgcagctga tccagcaaat ttaaagaaat caaagccaaa gcgaatcact tgttcttttg
    24721 ccgtaacatt tcgggtaatc acggcatttt tttcgctatc atccaccttt ttgatggata
    24781 caggatactt tgtttcatca aggctatatc cttcgggcgc ttttgtttct tgccaataat
    24841 actcgttgat agctaaatgt ttaacggcaa cttggttctt ttcgtctaaa gtcaaagtga
    24901 ccgtttcatc agaacctttc gttcccttcg ctaactctgg tttaaaagcc tcactccatt
    24961 taacagcttt tccatcctta gcagtaaaca aggtgtattc agcaccctca aagactgcct
    25021 taccttgtgc tttatcccca gtatctttgt cttctttcgt caaagtggtt tcgccagtga
    25081 cttcttgatt ttgccctttt acgttactgg taacaagggc tacggtttga ttggcatact
    25141 tcaattcgac ttttacaggt ttgaacgtat tcacgaaccc atgactagct ttagtttcgg
    25201 tcacataata ggttcccaat tccaacgcat tagcaatctc ttttggtgtt tccgcatgac
    25261 cgttttcatc cgtggtcatt tcttgaacga tttcaccggt tggactgtct ttgcgaatgg
    25321 caaacgtatt accagctaag gaataattgt cattccaaag atcactccct gtttcaactc
    25381 cgcttttatc cagaatgatt tgcccttttt cccgtgcgtt ttttgaagtc acgtaaatgg
    25441 tttcgcctgc tttaatggtg gtagtcatgg gggtggtatc aatcgtataa ggcgccggta
    25501 ctgatttttc ggtaatggtt acttttgtac catgaggaat ctcatccaat gtagcaatgc
    25561 cttctttgtc agtagtcacg tcttttgcag gtaaagtttt tccaaagtct aaatggaaaa
    25621 ccgttcctgg tacaacatca cccgattctt tatcgacttt tttgatcttt aaagaaccct
    25681 ccgtttccac atcaattttg attgtgtagg tattaggttt atcaatcgcc ccggccatta
    25741 aagtctgttg gccagccttt ttataggcga caggtgttcc ggtaccagca gatttcttaa
    25801 gcgtcagcac accactttct tttgaattgg catttggggt gattatcaac tgattaccat
    25861 tcacacgata atcaatgttt gcggcatttt ccacaacctc atcgaactct gacaaattca
    25921 gattattcgt atccgtcaca gtagtcgatt gccctaatac tgttttagcc gtactattat
    25981 ggaaggttgg tttcttctga taatcgtcaa tggcttgatt tatttttgcc tcaatggctg
    26041 cgatatttac ggcactacca tctgatcgtt ttattgagtg aagggtatac ccattaactt
    26101 cttgccagat catcttctgt gccacaatat gagtatctgc atctgtacct acttttttcc
    26161 ataaaacgga aactaatttg gctttatcgg acatatctgg taacgggttt ttctcatagc
    26221 caggagtgaa ctcattgtaa atcgggactc ctggttcaat acagaaaata tcttgtttaa
    26281 agccgttgta cactgcataa tgcggaacag tgacttctga cattggacga ccatcactaa
    26341 aagttccttc aacagcatat tgatgtgcta aatctagttc tacggttacc gtttgtggat
    26401 gggtaatttc atcagcaaaa gtaatcgtag gagcaattgc tccaacaatt gcttgaacag
    26461 ccaaactcaa aatagttaat acacataatt tctttttttg ttttttatac atgaaatgtt
    26521 ctcctttcaa attaagcaca aaaaaataag atgctcgctt gaacatcttt gtttataaag
    26581 tctttttaaa atttttattg actgaataga atggatttga gaatgaatga gcctaaaaat
    26641 ttaatctaaa atacgaaata tactgccttt aaattagaac acattttaga acacaaacat
    26701 ttcaaattta atcaaatata ataaaatata tttacgggaa aacctttata aatcaaggtt
    26761 tttcaaactt attcaatgat acgaagaaaa aaccaggtct taagaaagca cgtaaagctt
    26821 cacagttttc aaaacgttaa tcgttgttat accaacgatt tcagacactt tctcattgcg
    26881 agaaggtgtc tttttttcgt tctggtaatg agaaaggtaa tgacaatatt ataaaccagt
    26941 gtaagcaatg aatttttcta acgcatttcg ttgagctttt tcagtaacat gagaataagt
    27001 gttaagggta gtttcagcat ttttatgacc caatcgatct tgtgtttctt tgatacttgc
    27061 tccaccttct agtagaagag tagcgtgagt gtgccggaat ccatgaatag taacgtgctt
    27121 aaatttatat ttcttctcta aacgtttcaa tatattattt gaatagtccg catgcaaagg
    27181 ttgattaatg tcaccgtctg catttacata ggtaaagata agttgatttt ttgtatttgt
    27241 atttatgcca aactgcaaaa gctctcgagc ttgttctttt ttccagattt ttaacatttc
    27301 aatagtttgt gcatcaattg gtaatattcg ttcggaagat atattttttg tatttttttg
    27361 aatcctgaca gtttttcttc ttgcattctt ctcttcattt cgaatagttg catagctctt
    27421 tctaacatac attagctgtt cttcaaaata aatgtcagac caatgaagag cagctagctc
    27481 tcctttacgt ataccagtag taatcaaaag ctggaagatt aaaatatgtt ttaaaggctc
    27541 ttctttattg agaatatccc aaaaagcttt tagttcttct aatgaaaagt aattttcatt
    27601 ttctttcttt tcccgctttg gtagatttgt atttgccata ggatttcggt gtaatagctc
    27661 tgcaaaaatg gcatactcaa atatttgaga gctgtaagac ttaagctgtt tgatattctt
    27721 gaattttaaa gcccattcat tgactgcatt ctgacagtga acaggagtaa tcttagaaat
    27781 ggacttattc ccgaattttg gtagtatatg caagcggaaa aggtcttttg tacgttgata
    27841 tgtcgtttct tgtacacgat tcctataaga aggcaaccat tcttcataca ctgttataaa
    27901 caagtcatta gaaggcttat gatagccggt atcttcaatc tcgacctttt ttcttgcttc
    27961 gtgtaacttg gcttcttttt cagttttaaa cccttttttt acatagtatt cttgctttcc
    28021 attttcatct ttacgaacat acaagcggac actatatcgt gcaccgcttt ttgttgtgta
    28081 cttttgtatt gacataacta atctctccaa tctgataact tggcgagttg gctgattgaa
    28141 taattagttt tattgaagtt ttgatattaa tcgagctgtt atctctcttt ttagattgtt
    28201 aacctgtact tgggttaagt tgccaaataa ctcagattct aactcttttc tttcatcgat
    28261 atttaagttt aaagctttat taataaactt cttttgtttg gcaaatagaa tcgtgtattc
    28321 aaaggagtcc tctagaatac tttttaaaaa aagaaagtcg gagtcatctt cttgaagttt
    28381 gacttcaagt aaagaagtca tggaaaagta tagattagat aattcagtaa aataatctga
    28441 gtcaagactg gcaatattct tgactatttt gtcctttttt tctaaacgag cagtttttat
    28501 tctatcaaga ttaggttgtt ctatggaatt tgtttcgtct aatcccatca aataacttac
    28561 aggaacatgg aaataatcag caaggatatt ccatgtttca gtatttctag gatttcgctc
    28621 accacggtaa tagttcgtaa gagttgtata gttaatacca gtttctgtac taatttgttt
    28681 aatggtcttt ttactggaat caatataaaa ttttattcta ttcaaagtaa cgccccctat
    28741 agtaaattcc aaactattat agcatgaaaa ccacaaattg agtaatattt taattgatat
    28801 tactcgtttt gtggtttaaa atgtgcttat taagataaaa accacaaagt gaataatttg
    28861 aaaggaggcg ctgaatgata actcttaatg atcaattcat tagaagctta agaagacata
    28921 gagccgatct tatacttact aaaaacgatg cagcaaaatt gataggtata aatcggaaaa
    28981 catatgtcaa aatcgaaaat gggagcaaag aaagtattag agcatctact tatcaaaagt
    29041 tggtaaattg gttacttaat gatttgaaaa tctaaataaa tacaacttgg cgagttggct
    29101 gattgaagaa atagaaaatg agaggagaaa aataatggat gcaggaatta cagtaatgtt
    29161 agataaacaa caaacgaatc aattgaaaaa ttatgttttt gaaatgacaa aagaggctat
    29221 tgatcaagca agaattgata ccggattaga aagaccattt ctaaaaggaa aagagatggc
    29281 taaatatttg aatgtatcat atacaacttt tttgaaattt aaaagaatgg gattgccagt
    29341 tattttatta gaaaaaatgg agttgttttc aaaagaagaa tgcaaaaaat ggatattgag
    29401 tcatcaaatt taaaaaacac ctcgtttttt aaatttgatg aagtagaaaa aattaaaaag
    29461 agtgttcccg tcagtttgca gatcggggga acactcaata ataaagttct gagaaaactt
    29521 tatttgaatc tattatagca aataaaatca gaagcaggtg tgacaatgaa tgaaaaagaa
    29581 gttgaaagct tagttgaaaa gcaaggctat tctaattctg atatatatgc agaattatac
    29641 tatcaatatc ctattttttt aaacgtaaat gaaaaatata agaaaatgaa actaagttct
    29701 aagttagttt atatgttact aaaagataga aatagacttt cattgaaaaa taacttgtat
    29761 gatgagaatc aaaaaatttt tttgatattt gaaatcaaaa atctcgtaga aatttcggga
    29821 atgtcaaaaa atacaatctt atcagcacta gatgaacttc aaaaagttaa acttcttgtg
    29881 aaaagaaaaa atggcttcaa taaaagtaaa atgcaaatgt tcccaaattt ttattatttg
    29941 ttaaagcctg ttctgtgcaa agatgatgtt tataaaatta ataagataac ccatcaagaa
    30001 aatagacaaa aaaacttgat agttcaaaat ttgaactatg gaacaaatca atattctgtt
    30061 gataattcaa aatttgaact atgcgacaca tcaatgaaaa caagagacaa aaacaatttt
    30121 gctgatagtt caaaaaatga actagaatta tataagtctt ataaagatag taaagatatt
    30181 aaagaatcac ataatgatga tcttcttgaa tcactttcaa ctaaaaataa taaattagat
    30241 gaagaattga tcgaaacatt tattgaagaa aatcttctta aacaaatatg gggagaaagt
    30301 attgtgaatt gcttaaaact ggctagcaat agtgattatc gtcaattcga caactggtac
    30361 aaaaaattca aatatgcgat tagatctgct gaaaaagaac aaaaaattca actgaaaatt
    30421 atctatgaaa tttgtaataa caagtatttt gtagatcatg ttagagaaca gctatcaatg
    30481 actttacgtg acttgatacg aagagcaaaa acagatcatc gtatcaaaca aaaagacgat
    30541 tatatctttg cttctctaaa aaataaagcg ttagaactaa ttgaattgca aatcagcgaa
    30601 ggaatagata agcaataaga atagaattga gtcaaatgaa atggaggggt atctccactt
    30661 catttgagaa ctttgaatat aggaggggtt attttgatta aaaatcgttt aatagatcaa
    30721 gtttggtgtc tagatcaaaa tacagatagt ctcgaattaa tcgagactat ttgcttcaac
    30781 actatagttc tcgatttaag agctgaaaat gataattgtg taactcatgt cataattaat
    30841 caaaaaatgg ctcagaagct ctctgagagc ctcagaaaat gggctaaggg aggtaacgat
    30901 gaaaataatt gaaaaaaaga ttgccaataa aaaaataaat tggttggttg ttaattttct
    30961 tgttctgata gctatgatta tttttacaag taatattgct catgcgagta ttttcaattg
    31021 gtttgatgac acaaaaagtc aaacagagtt tttgaataat tctctttata gaccttacct
    31081 aaaaaagtac aatggagatt tccttagtca gttcgggatt tggctcggat gggctgtggt
    31141 aaaaggcatg tttactgtga cagattcgat tcaaaatatg attccagatg tgctggattt
    31201 gttcaatttc attgaaagta cagggctaaa taatgtttat caatcagccg taaatacaat
    31261 tgtcattgga ttaatgatcc tttcattgat gtttgttggt tacaaaatga ttactggaaa
    31321 aggaacagtg gatttaaaat cggttggaat gaacgtagtg atgtctatag cattaatact
    31381 tttaatgcca accatgattt ctagtggtat tcaatttgct aaaacatttt ataaggactc
    31441 tactacgatt acaaattcag atgacggagt cgcttggtca ttgataaaac aaggagtaac
    31501 agatttagca tatattaata aaacagatca gtactcaagc attgataaaa cggaggatcg
    31561 aaataattta acacgaaaaa attttcaaca gacggatctc acacaagtgt taacggataa
    31621 agtgattgat aaattggaaa aagaaaatcc aattgcggac aatcttcgtt atgaattagt
    31681 tgaaaattct aataatgaat ttgttgctac aaagttttct gacaacttct tatcaacatt
    31741 ttctgatagc ttgaaatcag gctattaccg ataccaagca aatctatggg ggatttcaat
    31801 tgggttgaca gcgttagcaa tcgcctatgt gttcagcgcg tttgtgatca ttacagcaat
    31861 actagaactt gcatttaaac gtgtgttagg tgttcttgtt tttgcaacag atattgagac
    31921 aggacagcgt tcaaaagttg ttctatcaga tattctacaa tgttacttaa ctgttgggtt
    31981 tcaaggattt gggttatcaa tgtttgctat gtttatcaat tttctgaact caggacaagg
    32041 tatctcaaca aatattttta taaaaacaat tgcttatatc tgtgccgttt ttgttttaat
    32101 caaaggcagt ggaacagtca tgagatactt tggtgttgat attggactca aagaaggcta
    32161 tgggcaactt gcttctgctt ttggaatggg cgcaatgctg tttagaaaag gatcaaatgg
    32221 atttaatcgt gctaaaggaa gcggagaagg agaagatcga aaaccagaaa agaatttcgg
    32281 cgaaactcta tcaaaaaaag ctggcaaaac tggaagagct ttgggatatg cccatgaacg
    32341 tggattatcc ggtttagctt cagatggagc tacaatggct agtgaaagag caacaaaacc
    32401 atttaagagt atgcgtgata tagctaatga taccaagaat aagtttaaag aaggtttaga
    32461 tgatggtaca gtgtccgcta ttaataaaaa tagcaaacct atgttagcta aaaataaaga
    32521 agatgaaact ggaaaatatg ctgattccgt gccaacacgt ctttcagata gaaaagatgg
    32581 tgctaaagtt gaaaatgccg atagaattat gtctagttct gaacgtatgc gtgaagcaat
    32641 gaaaaataat gctgaatcga ataataatcc tgcaagcgca attcagcaaa aagtccaaca
    32701 agatattgaa gaacgtaaaa atgctatgca cggtcaagct aagtctgctg aagaattaat
    32761 taatcagaag cgtcatgaag cgaaatacac gcctgaagca atgaatcgcg aggaaataat
    32821 gttaaggaaa cgtgtagaag ggcggacagg cgctaatatt gacggaaaag aagcgttgac
    32881 caaagaaaga attcaagaag ctaaaaattc aaatacagga ttagaaaaat tagtcaaaga
    32941 aaatccacaa aattctacaa gtagtcatgg tggccgatca gtagatgtcc gagaaaatat
    33001 tcaaggttca ctgaatggtc atgttggacg gaatgttgat gtaagagaga atttacaaag
    33061 ttcttcaaat gctcaaggta ctaagacaat tgatgttcga gaaaatctgc aaagtagcag
    33121 tacaagtcaa ccaaaaacag tagatgttcg agaaaatgta caaaattcta caagtagtca
    33181 agggtttaaa tcagtggatg tccgagagaa tgtgcaaggt agcagtgcaa gtcaaccaaa
    33241 aacagtagat gtccgagaaa atgtacaaag ttctacaagt agtcaaggat tcaaaacagt
    33301 agatgttcgg gagaacattc aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat
    33361 caatatcgtt gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat
    33421 tattgatagt gaaatccgtg aaaatgataa gggatcaaaa tcaagacgaa gattcactta
    33481 tgaagacaat gagttattca gagatacact taatgatcct aatccacttt tcgataattt
    33541 attaagaaat aaaagggggg gattgtcatg caattaattc taaagattag tacgcatgat
    33601 ttcttagaac ttgacgaatt atgtgaatca gtaaagaacg caacaaatga aaaaatcacg
    33661 gtcactaatt atattgaact gatgattcaa gaagaattaa agatgaaaaa tgaaatagag
    33721 agcaatttca aatgttgctc tctagaaaaa aatgtgaggg gataatcaat ggatgataaa
    33781 caaaaaatgt acgaaaaaaa tcgcaaacat agtgaggcct taaattttcg tgtcgataaa
    33841 aagacttatc ttgaactgac attgattgca aaacaaaggg gaatttctag agcagacgtt
    33901 tgccgtgagc ggttaaaggg actcaaatat gaaccgccgt tgttagctga agatgatagg
    33961 gtagttttaa ccaaacagtt aaatcaaatt ggtaacaatt tgaatcaagc ggtaaaggca
    34021 ttaaatatca ttgctaaata ttttagagaa gaatctgaag ataaaaaaga aaagagccta
    34081 cattttagtt ttgatgctag ccgttacaac atttttaaag atgaacttgc gaatagcata
    34141 gcaaaaaagt tgatgattag ttatccagat gacatttttt tggagtccaa tcggagtgcc
    34201 ccctcttttt ttaaat
//
LOCUS       DAPBBG010000027        33634 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPBBG010000027 DAPBBG010000000
VERSION     DAPBBG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.027"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..582
                     /locus_tag="RD477_001495"
     CDS             <1..582
                     /locus_tag="RD477_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7633858.1"
                     /translation="FYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTLEQLKEA
                     NADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYELGEEKQF
                     GEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRVSVVIPG
                     AKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(671..1930)
                     /locus_tag="RD477_001496"
     CDS             complement(671..1930)
                     /locus_tag="RD477_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDT7633859.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(2101..2547)
                     /locus_tag="RD477_001497"
     CDS             complement(2101..2547)
                     /locus_tag="RD477_001497"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDT7633860.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(2558..4771)
                     /locus_tag="RD477_001498"
     CDS             complement(2558..4771)
                     /locus_tag="RD477_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDT7633861.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(5004..5768)
                     /locus_tag="RD477_001499"
     CDS             complement(5004..5768)
                     /locus_tag="RD477_001499"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDT7633862.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(5769..6716)
                     /gene="prmA"
                     /locus_tag="RD477_001500"
     CDS             complement(5769..6716)
                     /gene="prmA"
                     /locus_tag="RD477_001500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDT7633863.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(6729..7217)
                     /locus_tag="RD477_001501"
     CDS             complement(6729..7217)
                     /locus_tag="RD477_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDT7633864.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(7183..7854)
                     /locus_tag="RD477_001502"
     CDS             complement(7183..7854)
                     /locus_tag="RD477_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDT7633865.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            7992..9278
                     /locus_tag="RD477_001503"
     CDS             7992..9278
                     /locus_tag="RD477_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDT7633866.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            9350..9544
                     /gene="ssrS"
                     /locus_tag="RD477_001504"
     ncRNA           9350..9544
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RD477_001504"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            9622..9906
                     /locus_tag="RD477_001505"
     CDS             9622..9906
                     /locus_tag="RD477_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633867.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            9979..10458
                     /locus_tag="RD477_001506"
     CDS             9979..10458
                     /locus_tag="RD477_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7633868.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            10681..10824
                     /locus_tag="RD477_001507"
     CDS             10681..10824
                     /locus_tag="RD477_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDT7633869.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            11402..12985
                     /locus_tag="RD477_001508"
     CDS             11402..12985
                     /locus_tag="RD477_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7633870.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(13422..14606)
                     /locus_tag="RD477_001509"
     CDS             complement(13422..14606)
                     /locus_tag="RD477_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDT7633871.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14631..15638)
                     /locus_tag="RD477_001510"
     CDS             complement(14631..15638)
                     /locus_tag="RD477_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7633872.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(15810..16169)
                     /locus_tag="RD477_001511"
     CDS             complement(15810..16169)
                     /locus_tag="RD477_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633873.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(16141..16512)
                     /locus_tag="RD477_001512"
     CDS             complement(16141..16512)
                     /locus_tag="RD477_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDT7633874.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(16529..16831)
                     /locus_tag="RD477_001513"
     CDS             complement(16529..16831)
                     /locus_tag="RD477_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633875.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(16848..17318)
                     /locus_tag="RD477_001514"
     CDS             complement(16848..17318)
                     /locus_tag="RD477_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDT7633876.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(17293..17571)
                     /locus_tag="RD477_001515"
     CDS             complement(17293..17571)
                     /locus_tag="RD477_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDT7633877.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(17568..18617)
                     /locus_tag="RD477_001516"
     CDS             complement(17568..18617)
                     /locus_tag="RD477_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDT7633878.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(18574..19503)
                     /gene="tadA"
                     /locus_tag="RD477_001517"
     CDS             complement(18574..19503)
                     /gene="tadA"
                     /locus_tag="RD477_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDT7633879.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            19635..21389
                     /gene="ade"
                     /locus_tag="RD477_001518"
     CDS             19635..21389
                     /gene="ade"
                     /locus_tag="RD477_001518"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDT7633880.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(21487..22149)
                     /gene="trmB"
                     /locus_tag="RD477_001519"
     CDS             complement(21487..22149)
                     /gene="trmB"
                     /locus_tag="RD477_001519"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDT7633881.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(22219..23001)
                     /locus_tag="RD477_001520"
     CDS             complement(22219..23001)
                     /locus_tag="RD477_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDT7633882.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(23205..24416)
                     /locus_tag="RD477_001521"
     CDS             complement(23205..24416)
                     /locus_tag="RD477_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7633883.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(24409..25146)
                     /locus_tag="RD477_001522"
     CDS             complement(24409..25146)
                     /locus_tag="RD477_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7633884.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            25284..25718
                     /locus_tag="RD477_001523"
     CDS             25284..25718
                     /locus_tag="RD477_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDT7633885.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            25711..26055
                     /locus_tag="RD477_001524"
     CDS             25711..26055
                     /locus_tag="RD477_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7633886.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            26235..27245
                     /locus_tag="RD477_001525"
     CDS             26235..27245
                     /locus_tag="RD477_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7633887.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(27314..28258)
                     /locus_tag="RD477_001526"
     CDS             complement(27314..28258)
                     /locus_tag="RD477_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7633888.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(28242..30962)
                     /locus_tag="RD477_001527"
     CDS             complement(28242..30962)
                     /locus_tag="RD477_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7633889.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(30959..32179)
                     /locus_tag="RD477_001528"
     CDS             complement(30959..32179)
                     /locus_tag="RD477_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDT7633890.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(32460..33305)
                     /locus_tag="RD477_001529"
     CDS             complement(32460..33305)
                     /locus_tag="RD477_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7633891.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        10029 a         6898 c         5840 g        10867 t
ORIGIN      
        1 ttttatatcc actttccgga tgaaagcaca ccaaaaaatg aatcagttgc tacattgcac
       61 gaattaaaag aagcaggaaa aatccgagca gtcggtgtgt caaacttcac gttagaacaa
      121 ttgaaagaag cgaatgcaga tggatatgtc gatgtagtag aagataaata cagtttgatc
      181 catcggcagg ctgaaaaaga attattccct tatcttgaaa aaaacaaaat cagttttgtt
      241 ccctattttc ctcttgcttc cggtttgctg acaggaaaat atgaacttgg tgaggaaaaa
      301 caatttggtg aaggagatcc tcgaaaaaga aatccagatt tccaaggaga acgtttcaga
      361 gaaattttaa ccgctgtcga tgtacttcgc cccattgcta aaagatacca agccactcct
      421 gcgcaacttg ttttagcttg gtacatgaag aatccacgag tgtctgtagt tatacctgga
      481 gctaagcgtc cagagcaagt atctgacaat gtccaagctt tagatttgca tttatccaat
      541 gaagattatc aaaccattga tgaagcattt cgtggatttt gatcaattga ccttatcaat
      601 ttatagtaat atgaaaaaag gaagatcagc actgtttgat cttccttttt ttcgataagt
      661 ttatgctaat ctaattcttg taaagtagcg ctcggctatt cggataagtc ggaaactgat
      721 tgaatggaat cgtccaatct tgttcaggta cagagaactc aaacttattt actagctgat
      781 cggaaaacac acgtagacta tgaagagtga tccattctcc tgcacagcga tgcatttgtc
      841 gaaaatttcc cccgccttgt gcaatcatct catattcttc tttataggaa atatcttttg
      901 ctttccctac ataccgtttg atcataaagg aatctggcgc ttcaactgtt cgttcatcat
      961 ggtctgttcc atataaatct aaaataaccc aactgccttc tggtatacga taaccatcta
     1021 cttctacttc tttcagcgaa atcgcaggta gcattgggaa gaaaggataa tagcgtctca
     1081 tttcttgaat aaacggatct tgtaacgtcg aaaagtcatt ttttaattgc tgatacaaat
     1141 ctggtctact gaaaagtgca tgtcccatca atgccgccca cacagttaga gcaaccgttg
     1201 gacggatgat gttcaataat tcaacagctg ccacttccag aggtaaaagc tgaccatcta
     1261 aatctgtggc attggcaaaa gcatataatg ccacattttc ttttccagcc acaggatttt
     1321 gtcgagcttc ttcaatcaag ccttgcgccc atttttctga ttttttgcga ttttcgacac
     1381 cttttatgtg atcaatcgga gaagtaaacg ttccgctgat catagagatt tgatattctg
     1441 ctaatttttc gacctcatct ttagaaatcg ctgataaatt gatacctgcc cactcgcaaa
     1501 tagatgtaaa gaaaaccatc ttacttaaat caaatagttc aaattgacca tgctgtgctt
     1561 caagtgcttg tgtcagattc ttatctaaga tacgatgata atcttccatt ctttccggtg
     1621 tcattagatc cataaaaata tttttccgat ggtgatgagc agctccatcc aaagtctgta
     1681 ctccaccttg accaaaaagt gtcttcaata ctaattttgg catagcacct tttcgtttga
     1741 aattccttgg atcataaaat acttttgctg ctgatgatcc gtagatcgtg atagcttctt
     1801 tattgaaaat ctttgctttg acgacgggag cgtccgcttc atggcgtaac tcctccaaca
     1861 tgttgtatcc tttttgatac aattttttta aatctgtgat tttgatatct actactggta
     1921 cttctttcat cttcagcaac ctcctataaa aaataacttg tataatttaa gcttaacttt
     1981 ttttccgact gctgacaaaa gaagcgcttt ttgcagacaa aaaagccact taagttagag
     2041 tttatcagtc taacttaagc gacgtgtttc atagttttta ctagtgaaaa ccagattact
     2101 ttatttgttt ttagaatcaa taatgatcgt tacaggtcca tcattgacga gagaaaccga
     2161 catatctgct ccgaattgtc cggtagcgac tgtgatgccg cgacttctga ttccttcatt
     2221 gaatgattcg tataaaggaa tagctgtttc tggtcgtgcg gcttccacaa agcttggtcg
     2281 atttcctttt ttcgtatccg caaacagtgt gaattgagag atacttaaaa tttgtcctcc
     2341 cacatcttct acactaatat tcatttttcc ttgttcatct tcaaaaatac gcatttgtgc
     2401 aatttttttt accagatagt ccacatcttt ttgcgtatcg gtttcatgaa tgcctaataa
     2461 aacaagtagg ccgtagccaa tttttccaat ttcttgctga tcgactgaca cagcagcttt
     2521 acttacccgt tgaacaactg ctctcatatc tgtcctccta tccattcgtc cggcgtacac
     2581 tgtagacatc aggtactgct ttgattttat ccacgatact ctttaggtgg gataaattct
     2641 ggataccaac cgtcaaatgg atcgtcgcca ttttatcttt attcgtccgt gcttcaacgc
     2701 tgttgagatt tttcgttagc gcattgactg tttgaagtac atcattcaat aagcctgaac
     2761 gattatagcc gtaaatttcc aaatctgcat catactcttt tcgtgtattc gatgtatctt
     2821 cccattcgac ttcaatcaaa cgttctgcta catttggttt gtcaggctga acattcggac
     2881 aatctcgacg atgaatggat atccctctgc ctttagtgat ataaccaacg atatcatcac
     2941 caggaatcgg attacagcag cgactgatac gaattagtaa gttttctacc ccttgaatga
     3001 caacgccacc ttcatgacgt actttcattt tgtcaggttc ttttttcggc tgattcatga
     3061 tttcttctgc ttcttgcttt tgctgctcga ttttttgttc tttacgttct ttttcagtca
     3121 gacgattggc catcgtcaag gggctaactt ctccataccc tacagctgca tagagatcat
     3181 cttctgtttg ataattaaaa cgatcgagtg cttcttgcag tttgttcttc gtcaaaatat
     3241 ctttaggcgt aaatcctaga tctgtaatac atttgaccac ggattcgtgg cctttgatca
     3301 cattttcttc tcgatcttgg gctttgaaga aacgtttgat cttatttctt gctttgctag
     3361 tagcaactaa tttcaaccag tcgcgacttg ggccaaatga atttggagaa gtcatgatct
     3421 caatgatatc tccgtttttc aatttgtaat caagctgcac cattttgcca tttacttttg
     3481 caccagtggt tttgttaccg atatctgtat ggatactgta tgcaaagtcc aatggtccgg
     3541 atccttttgg taactcagta acgtctcctt ttggtgtgaa gacgtagact ttatcactaa
     3601 agatatctcc ttttacgcct tccataaatt cagaagcatc atagctttcg tcttgaagtt
     3661 cgagtatctc atggaaccag cttaattgtt tcgtcatacc atctggttct actttttcat
     3721 tttttccttc tttataggcc cagtgtgcag caaccccgaa ttcagcgatt tcatgcattt
     3781 cctgtgttcg gatttgaatt tctaccggat ttcctgccgg accgatcacg gtagtatgca
     3841 aagattggta catattagct tttggcatgg caatataatc cttaaatctg ccgggcattg
     3901 gtttccattt tgtgtggatt gctcctaata cagcataaca atccttgatt gaatctacga
     3961 tcactcgaat agctagcaag tcataaattt cgttgaactg ctttttctga tctttcattt
     4021 tacgatagat tgaataaatg tgttttggcc gtccatagat ttctgcaaaa atccccagct
     4081 cttctgtcgc tattcgaata tcttcaacag taccacttac gtatttttca cgttcttctc
     4141 tcttcgtctg catcaagtgg acgatacggt aatactgttt tggatttaga taacgaagtg
     4201 ctgtatcttc taattcccat ttgatccggc tgatccctag acgatgtgca agaggcgcat
     4261 agatttctaa agtttcctga gcgatccttc tttgcttatc ttctcgcagg tgcttcaacg
     4321 tacgcatgtt atgtagtcta tcagctaatt tgaccatgat aactcgtaaa tcttgagcca
     4381 ttgctagcag catctttcgg tggttttctg ctagctgctc ttcgtgggat ttatatttta
     4441 ttttccctaa tttggttacg ccgtccacta acatagcaac gtcatcgcca aattcttctc
     4501 tcagatcttc taaagtgatt tctgtatctt caacaacatc atgtaagaag cctgtagcca
     4561 ctgtatgggg atccattttt aaatctgcta atatacctgc gacttggata ggatgaataa
     4621 tgtagggttc gcctgatttt ctaaactgtc cgtcatgtgc agcagtcgcg tattcgcatg
     4681 ctttctcaac aaatgctaca tgttcgggac ccatgtattg actaacgagc tttatgacac
     4741 cgggtcccgt taaaatttct tcttttgtca caggaatcga tccccctttc ttttttcggt
     4801 aaaacgggaa tcgggacaaa tatctgtttg ataattgtcc caaaatccgt gcagttatac
     4861 gtttttttac ggaaatgttc gctttttaaa agctttttca aacgattttt cgtatccatt
     4921 gtataaataa ttgtttctat tatacaaatt gttgactatt ttttcaattc tttctgtcga
     4981 tactctttta ttaatgaaga acgttagctg aaagaaggat tcattaattc ccattgatag
     5041 ctagcagcac ttaataaata aagaggtgct gtttctgtac gaaggattct tggtcctaat
     5101 ccacaaagta tccctttgtg ttctacaaaa taatcgactt catcgcggga taatccacct
     5161 tctggtccaa agacagctaa gattcgacta tctggttttg tttcactaag aattttggct
     5221 gcatttgatt tttcgccttg ctttgctgat tcctcataag caaccaagac cacgtcataa
     5281 tcatctagcc gctcaaataa atcttgtttc gtttccaata actctactga tggcacgatc
     5341 tgccgatggg attgttccgc tgcctcttga gcaatctttt caagccgttg ttgtcgtttc
     5401 atacgttttt ttgcatccca tttaacgaca gaggctgatg ccgggaaacc aagaaaagct
     5461 tgcgctccta attcagtgga tttctgaacg atccaatcca gcttttcccc tttaggatag
     5521 ccactcgcta tggtaatcga acttggcagt tccttttgat tttcttcttt tttaatttct
     5581 ttcagataaa cagcctcttc agtaatctcc gtgatttctg caacaatggc tatttgatca
     5641 ttgaatgcca aaaaaacttc atcctcgggt tccatcctca taacccgtac aatgtggtga
     5701 tagttttccc cttctaaaac aaaatagtct tttgattcat atgattcttt taaaaaatac
     5761 cgctgcatct attccacctc tggttttttc aaaataattg cataccagtc tttttgctgg
     5821 aaaatttgat ccacgataaa cccttgttct aacatggcat ccagaaccat ttgcttcttc
     5881 tcttcaatga ttcctgaaac gatcagcgtg cctgtttgct tcagtaaacg ccaagcatct
     5941 gggatcatca ataagatgat atctgctaaa atgttcgcaa cgattacatc actttcaatc
     6001 tcgattcctg tcaaaagatc gttagctgat acatggacat ctttggctat ttcattcata
     6061 tccatatttt ctttcgccga acgaacggct acttcgtcta aatcaaaggc atagacatct
     6121 tttgcaccaa gatattttgc tgcaatactt agcacgcccg acccagttcc cacatctagc
     6181 aatgtttcac caccacgcaa gactgtttct agtgctgtca atgtcaagga agtcgtggga
     6241 tgagtgcctg tgccaaatgc cataccagga tctagtgtga taattttttc tagaggatct
     6301 tctgtctggt agttttccca gcttggcgca accgtcagca ttcgcgtaat acgcaccgga
     6361 tgatagtatt tcttccaggc tgttgcccag ttgctttcct ctacttcact aactgttact
     6421 ttattttttc caatagctag accaaactca ggtaattgtg tgatacgatc tttgataaaa
     6481 ggcaagattt ccggtaagaa agtcgtttcc ggaaaataag ccatgactag tgcgccttct
     6541 ttcagcgatg taaaatcatt tttatctaaa atctcaccat attgatccga ctgaaaatgt
     6601 tcaacatcta aagaatcttc aatcgcgaca ccactcgccc ctgcctccat caaaatatta
     6661 gcaactgctt cgactgcttc actttcagtt tctactttta cttcattcca tttcatatct
     6721 gtcactcctt aataacttgg gtacggtaaa taagtatctg cttcattttg ctgttttact
     6781 tctactaatt ctgctagttg ctgattatcc cagtgaagct cgaatacagc ttcgtcatcg
     6841 tcttcatcca aaaaatcaag caaatcgctt tgcccttgag ctaatacttc atttagatat
     6901 tccactaaag aatcagctac tgattttgcc aacccttttt ttccttcata agggattact
     6961 gctagataat tgcgttcatc aaaaacagat ttctgaggat taaaaagaag tacgccgtct
     7021 tcaaattcga taattggttc ttgcgaaacg atcccttctg catcatcaat ctcaaattgt
     7081 gctttatttt ctgcaaacaa gcgaacaacg atctcgatcg tatgattttt tgtatcccaa
     7141 tctaaagcta catcatagtc tgtcacttgt ttttcaatta tttcatctaa atatgttaat
     7201 agatccttct ttttcatttt ctttcctcca accccaatct ttctcgattt ggttttttct
     7261 ttgtaaagag atataaggat ttcccgacac aacaaaacgc aacggctttt ctgtccatac
     7321 acctttgttt gggataccga ttctaggcaa agcattgatc ttttttggtg tctttttttt
     7381 ctcaaaaact aagcgtaacg aactatctgc tatagactga ccataaagtt cttgaggaat
     7441 tgctaatgct tccactaatt ttcccggacc gttgctgata tctggaccag tttttccgcc
     7501 acgattttta ctcatttgat caatcattgc ggcgggttcg attgcacgta tcattactcc
     7561 ttgaggaatc ccttctggct gagtaatgat gttcaaaatc cgatgagtat gcatcgtgta
     7621 aaggtagatc gtacctggtt tttcatacat tgcccgcaca cgcggtgttc ttcttaaacc
     7681 atagctatgg gcggcctcgt cttctggacc taggtatgct tccgcgtcta caatatatcc
     7741 agctaatctt ccttctggtg tgtcatgctc caaatacatc cccaataaat cccgtgccac
     7801 ttctggcgtc gttttattcc aaaaaaattc atctatcttt tgtttatccg tcatttttct
     7861 cacctacttt atcatacaca atattagttg gataagctat gaaaacaaaa caacagaagg
     7921 ctgttctttc taggcatctt gctaaatatg atataattaa gggacgtaaa aacaagaagg
     7981 aggaaaatcc tatgcaacag ccattggctt atcgcatgag acctcgaacg atcgatgaag
     8041 tcgttggtca gcagcatcta gtcggggaag gaaaaatcat acgccgcatg gtcgatgctc
     8101 gcatgctctc ttccatgatt ctatatggac ctcctggtac tggaaaaacg agtatcgcaa
     8161 gtgcgatcgc cggctctact aattatgcat ttcgcatgct aaacgccgct acagattcaa
     8221 aaaaggatct acaagttgta gcggaagaag caaaaatgag tggtacagtg atcctcttat
     8281 tggatgaagt acaccgatta gataaaacaa aacaggattt tctgcttcct catttagaaa
     8341 gtggccgaat catcttgatc ggagcgacta ctgagaatcc ttatattacc atcaatccag
     8401 cgatccgcag ccgtacacag atctttgaag tcaaaccatt aacagaacaa gacatccagt
     8461 tggcagtcga acatgcttta agggataaag aacgcggcct tggacaacaa gcaatccagt
     8521 tagacgaaga tgctcttctt cacctttcgc gtgcaacgaa cggcgatttg cggagtgcat
     8581 tgaatgggct ggaactggct acactatcga ctccttcaga taaagaagga aggattcatc
     8641 ttacactgtc gatcattgaa gaatgtgtcc aaagaaaagc tcttacccat gacaaaaatg
     8701 gagatgctca ttatgacgtt atttcggctt ttcaaaaatc gattcgtgga agtgatgtgg
     8761 atgctgcact tcattatctc gcccgtttag ttgaggcagg tgatctggca agtatttgcc
     8821 ggcgtctgat ggttatcggt tatgaagata taggtttagg taatcctgct gctgcggctc
     8881 gtacagtgaa cgctgtatta gctgctgaaa aattgggatt gcctgaggca cgtatcccac
     8941 tagcagacgt cgtcgtcgat ctttgcttgt cgccaaaatc caattctgcc tacatggcat
     9001 tagatgcagc acttgctgat attcgagagg gcaaagctgg agatgttcct gatcatttga
     9061 gagatagcca ttacaaaggt gcgaaatcac tcaaccgtgg cgtgggttat cagtatcctc
     9121 atcattttga tcaggcttgg gtcaatcagc aatatttacc agataaactg aagaatgctc
     9181 agtattatca gccaaaagat acagggaaat atgaacaggc tctgggtcaa cagtattacc
     9241 gtataaaaga gtggaaaaag catcctccaa aaaactagac aagaaaacgg ttctgtttct
     9301 gcgagctctc tcattgctct ttttaaattc atatgctact atagaggtgg gaaatctgtg
     9361 atgttcgaat tgtcctcttt gtgtgttgac cgaacatttt tattgatatc ttgggacccg
     9421 tcttgtgtca ctgctggtaa catgccttat catttggtag ttcgaagcta cgaccatggg
     9481 acccacctgc ttgaattcgc gggatcaata ctacaggaca aacaacggca ttaacggaga
     9541 ttaccatctt ttaaagaata aagcaatttg ctcttggcaa attgcttttt ctatttgttg
     9601 cttattttgg aggatttgtt catgattagg attttaatga ttatcgccac tttagtatta
     9661 ttattcgtta gctattattt atttaataaa caggatatct tttttgtatt gatcaacaaa
     9721 aatgataaaa atcaaggatt cctacaattt tacggagctg catatgctgt tttaggcgta
     9781 atgggtattt taacagcttt tttcaatcaa cgctttattg ccttggtctt tttactgatc
     9841 gtaattattg tttctgccac cttcagtatc cgatttgcaa aaaaaattgc tgaaccaaag
     9901 cagtaaataa ttctcttttt ccctaaagtg ttctacaata aaagagtaca atacgattga
     9961 cttgaggagt gaatgcttat gttacaacaa tacaaaaaaa tcatggttgc cgtagatggt
    10021 tctgatgaag ctgaattagc ttttaaaaag gcggtcaatg tagctatccg aaataacgga
    10081 gaacttctat tagcacatgt cattgacaca cgttctttcc aaaccgtttc atcctttgat
    10141 ggaatgcttg cagaacaagc tactgaaatg gcaaagcaaa cattggcaga ttatgaaagc
    10201 aatgcgaaga aagctggctt gaataatgta acctctgtcg tagaatatgg ctcaccaaaa
    10261 caaattattg ccagagaaat cccagaagat aatcaagtag atttgattat gttaggagcc
    10321 acaggattga atgctgtaga acgtttattt attggttctg tttctgaata tgttattaga
    10381 aacgcagctt gcgatgtgct tgttgtccga actgatttag aaaaccaatt gccagctaaa
    10441 gaccacacca aagcttaaca cgtagacgac ctgccaacta tcggcaggtt tttttgcaca
    10501 aaaaaccgtg ataaaaacaa aagacgcggc aagcactcat cggtctttat aatgtttaaa
    10561 ataaagggaa aacagtcaat gagttcaagt aaaataaaaa aaagtgaaca ccagggcgat
    10621 tagtcgccct ggtgtggttt aaaacgacct aagcatgcca aagcttattg gttcgtcaat
    10681 gtggaagttt ccgatgaatc tgatacgaaa aaatatgaag tatcgatggc ggataccgat
    10741 tttatcgcca aactaaaatt ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca
    10801 ttcggtaaat cattcaagaa ttgatgaaag gctaccgctt gccattgcaa ccacaacgat
    10861 actcctgaac gcgatgttgg tgagatttcc aaagagaaga gctgtttaat gcaacggtat
    10921 taagcaaatg gcaaaaggaa gtcatgtaga caaagttttt tatcaaacac catatgattt
    10981 cgcctgaacg ctggtttagc tcagaataca atgatgtggt ccacgagaca ttaccctcag
    11041 tcagataaaa aaagctctct gacagaatta cacatcgata aatattaagt aaatgtcagc
    11101 gctagttgaa ctttctgaca ctttcaggta cactggaaaa gacaactaca cgaaggaggg
    11161 aaaatactat gatgcgcatg actaaaataa caagtaatga aaattaccaa cgataccata
    11221 tcgcatttag ccatgcaatt ggttttacag ttgctgttga cgggacaagt ttgtccaaaa
    11281 cagttgaatt cacgtaaaat caatggccat ctcaaggtat ttttctcatg ataatctgag
    11341 tttaaataac acaggccatg gataaccatg gcctttttga gtggtctcaa ggagagagat
    11401 catgatcata caagcaattt ctattaaaaa aacaatcggc gggaatccat tatttgagga
    11461 tttatcctgt caaattgaag ctggtgaaaa aattgcctta gtcggtatca acggaactgg
    11521 caaatcaacc tttttacaaa tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag
    11581 tcgcaaaaaa ggcttaaaga tcgggacggt cgaacaagaa cttacagttt ccgaagcgac
    11641 cgtcaaccac tacttgctcc attctgccgc tgagatacag gacctcaaac agcaaatgag
    11701 tcgctacgaa gcattgatga cagaaccaga tacaaactta gagaagtgtc tggcccgtta
    11761 cggtgagctg cagcatcgct ttgaagaact aggcggttac gtattagaag accggattgc
    11821 agcgatcttg caaggcttag ggattccaca ccgtcaggaa gcacggttaa cagaacttag
    11881 cggaggcgaa cgagttaaag tcgctttagc caaaatttta gcagccgatg ccgatttact
    11941 tttactcgat gaaccgacca atcatttaga tcttaacagt attcgctggt tggaaaacta
    12001 tttacagaat acaaaaaaat cggttctggt cgtttctcat gatcggcaat ttctcgatca
    12061 agtcaccaca aaaacatggg aactggaaga aggtgcattg attgagtatc ccggaaacta
    12121 cagccgtttt cgagtcttga aagaagctcg cttggctgag ctgacaaaaa attacgaact
    12181 gcagcaaaaa gaagtccagc ggttgaaagt catgattcgt cgttttcgtc agtgggcaca
    12241 tgaaggagac aatgagtcct tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa
    12301 actgacactc gtcaaaccgc cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg
    12361 tggaaaatct ggcaaagaag ttttcatcat tcaaaacttg caccagcagt acgctgatca
    12421 agtactattc aaagacagca gttttgcagt ctatcgtgga gatcatttag ccatcatcgg
    12481 cgacaatggt gcaggaaaat caaccttact caaattatta ctcggtgagg aaaagcctta
    12541 cggtggcacc atcaaattag gtagtagctt gcaaatcggt tatttgccgc agcagctgca
    12601 gtttgccgat cccgatagcc gtttgctggc ttacgccatc acattgactg gaaacgaaga
    12661 agtcgctcga cggcaattag ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat
    12721 caaagattta tccggtggtg aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa
    12781 aatcaacgtc ctgattttgg atgaaccaac caatcacctc gacagctatg ctcgagaaga
    12841 aattgaagaa atgctgcaag actatcaagg aacgctccta gcagtcagcc atgaccgcta
    12901 ctttttgcag caacacttcc aagaagcact cgttattgat caggagcaga tcagccgcca
    12961 gccgctcggc caactgattg actaggacgt tttttcgcaa caaaaaactt gattttcata
    13021 cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat caagttttta ttattcgatc
    13081 aatgaatgtt tcggatcggt tttctagcta aacataaagc gaagaataaa gtcgacagcc
    13141 cacgaggctg atctaaaaat gttttttctt tttacatatg taatgtccct tataaagatg
    13201 aatgaaaata aaatattggg attttcctga aaatcaataa ataaaatgcc agtcttttcc
    13261 cttttgacca cttttccaag caaaattccc taagaagatc ctgtaaaaaa gaagggaaat
    13321 tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct
    13381 cttgcatgcg tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc
    13441 acatcacggg caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat
    13501 tcttttgtag agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca
    13561 caaccaaacc aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg
    13621 ataccagcag taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg
    13681 tattttcgga tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag
    13741 ttcttttcaa gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc
    13801 ttgttcaaaa cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg
    13861 agaacagctg gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg
    13921 aatcccatag aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt
    13981 cctaagtggc aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca
    14041 cgttccgaca cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct
    14101 tcataataag acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa
    14161 gatgtatcga atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc
    14221 cccattaagt ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc
    14281 attactttct catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg
    14341 tgccctactc cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct
    14401 aacagcattt ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga
    14461 ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct
    14521 ttcgcaacta cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca
    14581 gcattgattg caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca
    14641 gatgatttcc agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat
    14701 gaagcaagtt tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt
    14761 aagatcaaaa tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc
    14821 attccttgaa gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt
    14881 tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt
    14941 gattgctcta gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac
    15001 tcttttgctt tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg
    15061 acaggatcta ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt
    15121 gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt
    15181 ccttctgttt ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc
    15241 attcctgccg caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca
    15301 ataagaaaaa caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa
    15361 tgttctgtct ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc
    15421 tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt
    15481 gtcggcactc catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg
    15541 acataggctt ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg
    15601 ttttgattaa acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat
    15661 attataacaa ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt
    15721 ttcactgttt tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca
    15781 gaaaagaggg cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt
    15841 acttcttctg tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt
    15901 tgtacccact gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg
    15961 gcaagcgtag actgttccga taaagccatc tgcttcatta tttttgctat ataataattt
    16021 ttgattcgta tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa
    16081 aacgtacata aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt
    16141 ctactgatcg ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata
    16201 aaaagaaatt gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg
    16261 ttgatgtccg ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata
    16321 ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct
    16381 cttgagttct tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg
    16441 caaaaattca cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact
    16501 gctcaatatc atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt
    16561 tttcaatact gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat
    16621 tatcgacaat ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat
    16681 acatccgctg cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac
    16741 ttttcttttc tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag
    16801 aagcgatcat aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt
    16861 tttgtatagc gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc
    16921 caataaaatt gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata
    16981 gaagcatggc gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta
    17041 tctggattcg gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt
    17101 tggatctgat tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg
    17161 aaaaattgat agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa
    17221 aggatcgttg taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct
    17281 aaaaccttac cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc
    17341 attcaacaat tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt
    17401 ttgtgtgtct accaactgga tgatcgcttc acgactttct tggtctacat gattcctata
    17461 acgcgacaaa ttagggacga acaacaacac taatacactg atgatcagta agacgattag
    17521 catttcgatc aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc
    17581 tcctctatgc ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc
    17641 gctacgccca aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa
    17701 agctgttttc ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc
    17761 tctccctgta aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc
    17821 tcagccaatg acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc
    17881 agactttctt gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca
    17941 tgaaaaagct ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt
    18001 ccaatcaata attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga
    18061 cgatacctta ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa
    18121 tgaaagactt gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata
    18181 agctgaggca aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat
    18241 agcatcggat atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac
    18301 tgtttggcaa tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc
    18361 tgtaccagtt gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct
    18421 tcctttagct gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt
    18481 aataaataaa gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt
    18541 gcaaactggt tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt
    18601 ttcctgagct tcttcataac tttcctccca tgattttttt actgattctt cctccataaa
    18661 ttgataactc cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat
    18721 gacaccagct aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac
    18781 caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc
    18841 tgctcttacc gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc
    18901 tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt
    18961 gatctgtagc tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac
    19021 ttgcaatttt tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgg
    19081 tccgctgaaa aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg
    19141 agaactaaag caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact
    19201 ctctcgttga aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa
    19261 agaataagta aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa
    19321 atacttaaat cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt
    19381 tcgttttttt ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac
    19441 gtcttgggca tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc
    19501 cattattctc ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat
    19561 gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt atacttataa aaatttatct
    19621 tgggaggcac tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa
    19681 agcctgctga cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca
    19741 tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa
    19801 aaagcgtaca taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc
    19861 atatcgaaag ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag
    19921 ttactactgt agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg
    19981 agtttatgat agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt
    20041 gtgtacctgt aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc
    20101 cttttttaca aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag
    20161 caaataatga aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa
    20221 ttgacgggca tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta
    20281 ttcggacgga tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta
    20341 tgtatttaat ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga
    20401 tcacacctga aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt
    20461 tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc
    20521 cattacaagc cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac
    20581 taggtgcgat tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc
    20641 tgccaattca tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag
    20701 accatttatt tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc
    20761 aaccattaaa tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg
    20821 aaattatccc gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg
    20881 aacgattcat tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc
    20941 atcaaacggg aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga
    21001 tagcgacaac gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg
    21061 atatgctgta tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca
    21121 aacagcagga aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt
    21181 tttcgatcgt ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag
    21241 ctaaaacctt tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat
    21301 tgaagatcac cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg
    21361 gaaaaaccga tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc
    21421 tgtaatgttc attttctggg atttatttat ggatattaat gtttttatga aagcagaaag
    21481 ttagtttcac agacttttct ttttggtctt atcttgaaat tgagcttcta ctcgataaat
    21541 acgttgacct tttgaagaaa atttttcttc atactccgtc ataatattgc cttcaaattg
    21601 actttgatgt aaatctaacc atacctgttt gaggatcatg ccatattgag aaaaacttgc
    21661 cagagaatac tcaaacaatc cttgattatc tgttttgaaa tggatttctc catttggacg
    21721 aagaatctgt tcatctacag caagaaaagt cttatatgtc aatcgccgtt tttcatgacg
    21781 tttttttggc catggatcgg aaaaattcag atagatttga tctacttcgc tgtcagcgaa
    21841 atactctgtc aacgctgatc catccacatg tagaagtttc aggttaggaa gcggttgttc
    21901 gatcaacttg tctagtgcaa tcgaaacaac actgacctgc atttctactc caatataatt
    21961 gatttcagga tgggcttttg ccattccagt gatgaactgg cctttcccca taccaatctc
    22021 aatgtggatt ggatgatcgt tttcaaacaa ttcattccat tttcccttcc acaatgttgg
    22081 atcactaatt acaaaattcg ggtgagccgc taacatttcc gctgctcccg gacgatttcg
    22141 gacacgcatt atttatctcc attcctttcc tctaattaaa aaggtctggc caaatctccg
    22201 cagagtttgg gccaaacctc agccactgaa aattcgtttt aattgtaaaa tttcttcgtt
    22261 catccgtcga tgatcttcac gtttgcaata tctggtgatt tcttggagca aattcatggc
    22321 cgcataccaa tgaaattttt caagcatttc gtcagtcggg cgtatgccat aactgatcag
    22381 ccactgattc cagctagaca gtggcacata atttcctaaa atcgttgcaa tatccaccgc
    22441 agggtctgca aacatgattg aatcccaatc gactagatat aaataatttt tacatactaa
    22501 ccaattacga tggtttacat ctccgtgtac tactgataag ttttccttat ctattgacgg
    22561 gatctgctca cgaagaaaac gatggacaat ttgcagaaat tgatttttct ggagttcttc
    22621 cggtaattct ttttcatatt tttgcagcat cttctccggc gtcatgacac gaccaccgat
    22681 acgggtcagc atggttttaa gagaatttga atgatgtaaa tggtacaaaa catcaatcac
    22741 gtcgtttctt tgaccaatct cattaggagt cagcaagcga ccttccagcc actcttgtgc
    22801 tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca agttttggtg taattccttc
    22861 ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta ataaagactt tgtcctcatc
    22921 acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg
    22981 gtctaactga aactccatca tgcattctcc cgtcctctta ctctttgttt tgcagtactt
    23041 attattctag tcgcctgaac actacacgtc aagcacatat acgttctttt tcatttaatt
    23101 gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa
    23161 cgctctattt ttcattctca tttttaccct acacagcttt gttttcagtc tgacatcttc
    23221 ttcaaacgag ttggtacata tagtttaaca aagagcatag taacaatcaa ataagccgct
    23281 agcggaaccc atcgctgctg cccatctaat acgactgcag caatcaaacc aaaaataacc
    23341 gacgcgataa ttaataccca tgtgagtaga tactgaatcg cctgcttctt ctgattctta
    23401 tggattggat aaagctgaac gagaaccata tagcggaact ggttcgctaa aggtaacagt
    23461 tggaaaccga tcaaatataa aaataagaaa ccgataatca gactaaaata aaagtcgact
    23521 actccggcaa tcaataaccc acctataatt actaatcgga aataaagacc actaaaatca
    23581 gaacctcttg ccaatcttct tgcaaacaag taaagataag tgttttttga ttctctttta
    23641 atatgagtca atataggatc caaatagcgt cttcttttga tctttgccgt aatttcagga
    23701 acatctgtga ataaattgat aaattgatag atctgatgca acctatgttc ttctacatgc
    23761 accattttct cccaatccaa tcctttttct tgttgcttcc acaaaaacca gtaatataga
    23821 cttgcctgta ctaatgcacc tgctattcct gcccatatta gccaataaaa tgatagaaac
    23881 aaaaagatca gagaagaaaa aaaccagcct aatttccaat aacgaataac ttgttgatct
    23941 agtcgaaaca attctgctct cttgatttgc agatgactga atttcaaact ccataatatg
    24001 atcagataga cgaaaaacat cgaaaaggat tgctccgtac taaccaccac taatggcatg
    24061 gccaatccgc atgccaatat caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct
    24121 cgttccaaat aggtttgcat ttctttttct ttaggaagta aaaaaacaat atctgctgcc
    24181 tctgcaaaag tagccatctt acccacgaat aaacagagca tccaaaatac agagattatg
    24241 agccctccaa ttgcaaacgg tggctgcagg gttttcaacc aattagaata atataatccc
    24301 acaccgccaa tcaagaaaag acaaactaaa acaaaatgat cgttcaatac ataacgcata
    24361 tatttcatca ttttcttttg atgtcttgct aaacgttttt gaaaaaaact actcatgtgc
    24421 ctgctccttt gttaaagcaa tatagatttc gtccaaagaa gaatccggca gctgaaattc
    24481 ttttctcaac atctcaagag aaccgtctgc tcgtatctct ccgttatgta agaccacaaa
    24541 acgatcacaa tatttttctg cagtagctaa aatatgcgta gacatcaaaa taccggctcc
    24601 ttcttttttc atttctacca tcaattcaag caatgcattg atagctagtg gatccaagcc
    24661 taaaaatggt tcgtcgatga tatataaaga aggctcgatc aaaaaggcgc ataagatcat
    24721 cactttctgt ttcattccct ttgaaaaatt agctgggaac cagtcaagac gattagataa
    24781 ccgaaaagtc tccaacaact tttctgctct tttcattgct atatccatag gtatatcgta
    24841 ggccatcgca gtgacttcga tatgttctct caatgtcaat tcttcatata aagaaggagt
    24901 ctctggaata tacccaattt gtttgcgata ttcttccgta tttttcttta aggatagatg
    24961 attgatttct atcgttcctt ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt
    25021 acttttccct gctccgttca gtccaatcaa ccctactatc tctccaggct gtacatcgaa
    25081 actgatatct tttaacacag ggagatgtcc gtacccccct gtgacatgat cgataattaa
    25141 actcattctt tttcctccgc catttatttt tatttattat accacgaaga aaatgacggt
    25201 attttgtttt tttccttaca atagctttaa cttatggtaa agttaagctg aagtatttat
    25261 tctttataga aaggatgttt tatatggaaa actgtatttt ttgcaaaatc atcaatcaag
    25321 aaatcccaag ctataaaata tatgaagacg acaaggtcta tgctttttta gacatctctc
    25381 aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca tgtagcagat atttttgaat
    25441 atgatcccga acttgcagga gaggtttttt cgcgtgtgcc taagattgct caagcattgg
    25501 aaaaagcttt tccagaaatg aaaggtctta atatcttgaa taacaaccgt gaactagcct
    25561 atcaatctgt ttttcattca catatccatt tagtccctcg ctacagcaaa gaggatgatt
    25621 tttctattca ctttgtcaat catcaagata gttatggatc ggaagaacta aaagctattc
    25681 aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa actttttcaa aggactgtta
    25741 tttggagctg ctgctggcac tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa
    25801 acaagaaaga aactgatcga agaattggaa gaagcaacaa ctctgacaaa tgatttaaat
    25861 aacagcctga atcattttaa aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg
    25921 attcctgctt tccaagaagc aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa
    25981 ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa aagctgatct gccagagtat
    26041 cctgaacaaa tataataatg attattttct caacagattt aactatataa gtgagaaaag
    26101 tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt gtttgaaact accacgacaa
    26161 cgtttttaaa ggaaatggga tatggtatag ttaatatata aaaataataa ctaattacag
    26221 gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc agctactagt gcgttggctg
    26281 ttttaactct agcagcttgt tcaggcgata ctaataaaga tatcgcaacg atgaaaggcg
    26341 gaacgatcac tgtatctgat ttctatgatg aagcaaaact tgaatcatcg aaccaatcct
    26401 tagtacaacg gatgatcatt tataaagtat tcaataacaa atatggcgat aaagtaaccg
    26461 ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc attaggtgat acttttgaat
    26521 cacaattaga agctgctgga tatacgaaag atacttataa agaatatatt cgtaacaatt
    26581 tagcttttga agctggcttg aaagctcacg tagatattac agatgacgat ttgaaaactg
    26641 cttggaaatc tttccatcca gaagtagaag cacaaatcat caaattatca agtgaagatg
    26701 aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga tttctctaaa ttagccaaag
    26761 acaaatcaac tgatacagag acaaaagaag atggcggtaa agttaaattc gactcaacga
    26821 caacaactat tcctgcagaa gtaaaagaag cagctttcaa attgaaagac ggtgagatct
    26881 ccgatgttat tactacaaca aatcctactt cttatgcgac agaatactac gttgtcaaaa
    26941 tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata caaagatcaa ctaaaagata
    27001 tcgctactga aactaaacta agtgacaatg cattcacaac taaagtaatc ggtgaagaat
    27061 taaaagatgc aaatgtaaaa atcaaagatg atgcctttga aaatgtgttg agtgcattca
    27121 caacaacttc atcaagtacg aaagactcaa gtgaaactac agcttcaaca aagagttctg
    27181 acacaaaatc aactgattca acaaaagaat cttcaactga agaaacaact gattcatcaa
    27241 aataaaagaa aacaaaaaaa gcgaagactt atgtctcgct ttttttgttt tgaatgaaaa
    27301 aaataaattt ttgctataaa tcttttggta catagaaact tcggttgtcc ataccgaaaa
    27361 tccgatcggt gtattttcct ggttctgttt gacgaatcga agtcaacatc atctgcatcg
    27421 aagcatcgat attgtcgatt tgatgtaata tttctgcttc cattattctt ggacgtacag
    27481 gtgagccata ttctaataag ccatggtgtg ctaaaaccat atgacgtaat acgagaacat
    27541 cttcgtcagc ttcgctgatt ttcaacgcaa gacaggcttt agttatttct tcgtcaatca
    27601 ataccagatg accgatcaaa tttcctgcaa gcgtatattc tgtcgacata ggaccggaaa
    27661 gttcaatcac ctttcctaaa tcatgcaaaa tgataccagc atacaacaat gctcgattca
    27721 gctcaggata ttcatcggcg attgatttcc ctaagtgaag cattgtcaaa gtatgataag
    27781 ctaatccact ggcaaatgca tgatggtttc tttttgctgc cggaaactca aaaaattgtt
    27841 tttgatattt attaagtaga tagcgaacaa tacgattcca atgcgcattg gtgatctcaa
    27901 acaacaattg attgatttct tcttccattt cttctttctt caaaggggct ctttccatgt
    27961 ataaactagg ctctcctggc tcatctaccc ttgttaagcg catatgaaga atctttactt
    28021 gagggtttcc ttgataaacc tctcttttac cgtttagaaa aacgactgtg ccggctgtaa
    28081 atttattgat ttcctcctcc gaagcatccc agtatttacc atcgatagta cctgaagtat
    28141 cttggaaagt aaaagcaata aatttttttc cgtttttagc gattcttaca tctgcacttt
    28201 tgatcaggac aaattgttca aactgctcat cgattactaa atcacgaatt tttttcatta
    28261 tttgctcctt ctaattgttt tacgatttgg ttcatctcat ggtaatagct taccatttct
    28321 ttatcagaag aaaaacaaat tatttgatac ttttctccaa atttagctaa aagttcagct
    28381 aattgatact ttcgctgatg atcataatga agccagccat catcaatgat gatcgggcat
    28441 atcgaacggt tagcttctaa tgagaggtaa gcaaatctca tcgccataat cagctgatct
    28501 ttcgtacctg tagataactc atagatggga aattctgtat atccgtctgt taaagtcagt
    28561 gtatcatttt tcagtaatat cttctgataa tttccttttg ttaaaatacc taaataatca
    28621 cctgctgctc ttaacaactg gggtaattgt ctttcactca tctctgttga aagatcacct
    28681 aagaatgtcc cggcgatttc aagtgctccc cattcctcca gtaattcctg taattttgct
    28741 ttttgccggc tagcttgttg ataaagttcg tccaatgtgc cgtctctttg caactcttct
    28801 acttgaagtt ggagtcgttg ccgattttca cttgcttctt tttcttcttg ctggagttgg
    28861 gattgctttt ctttcagttg gcgcagtctt acagataatt tctcttttgt cagctcttca
    28921 ggaaatagtg gagtaagcat ttccgataat tcattgtatc ttttcatctg ctgttcggct
    28981 tgtttggctt tttgtaaaaa gacaggtact tccgaaggat aagataagcc tgcttgagtc
    29041 aattgttcgc tgtattcttg tatccatact ttttgtttct ttttcaattg attgatttct
    29101 tgcttcaata aagtattttg ctgatagctt ctggcaaatt tcatctgttc catctcttgt
    29161 ataaaacggg aaagcaatac cattttttct gaaattgtct tatcttgtat cggtaaccat
    29221 tctttaacaa actcaaattt ttggtcgatt tgtcgtagtt tttcttgtat ttcctgttct
    29281 cttttatgtt tccgctcaaa tacgccatat aatttctgat aatctatgac ttcctgtcca
    29341 tattctttca tgagtttgag cgtatccatc tttcctaagt gccgatgatc cgcaaaacga
    29401 tatacttctt gctgaagaat ctgtttttct tctccagttc tcttttgttt ttcttctgct
    29461 tcagctaatt gagctaaggc tagatcaagt tgtcctagtt tttcttgcca cattcctttt
    29521 atctcagaga aatcattttt agttttaatt tttggttgcc ttttgtagaa gaaatagcca
    29581 atcatgcctg caccaattag taaaaggatc actttcaagg gcattggtac aaatatacct
    29641 gcaatgatta ttgctccgcc agccatcagc atatacggtt tagatgaaga gactggtgct
    29701 atctctgact gattttgtaa taatgctgga taggtctctt ccaagtcatt cacttctgct
    29761 tcaatcatct cttgctgttc tttagctatt tggagacgca cagaaatctg gctttctaga
    29821 tcagcaatcc ttctttcttc agccagaaga tgcttccatt cttcttcata taaaaattct
    29881 tccggtaacg gatttttctg ccaatgccac ctttgttcca aaatcttcaa ttctgaattt
    29941 gtttctctag tctcaatttg taatctttgc cattcttcgt ttaattgttc cgctgtaaat
    30001 tgaagttgct ggagatcttt tagttcctgc tcctgctcta aataaaaata atatctggct
    30061 gactgctgat ccatcccgct atgcttttcc agtgttgctt ctaattcttc caaacgctca
    30121 tttgtttgct gataccgttt ggagaaagac tccagatcta aaagaagttg ctcatcagga
    30181 agacctgtag tgctgttttt tttgagagat tgccattctt catataaaga aaaattaagt
    30241 tcttgctgtc tggctaatga aaattgtttg ttcgcctctt ttaattgtgc caataactgc
    30301 tgctgttgcg actcgtattc ctgttcttgt tggatcaact tgccaaaata agcttcctgt
    30361 tcttgttttt gatgaatttt ttcttgcagc tctttccatt catttaattt ttgattgatt
    30421 agtaattttt ggcctttttt cttgaataat tgttgtgctt tctgataata ttcttgccgc
    30481 ttttggaata cttgagaact gcccgtgatg cctaaagaaa tcagcgcatc atgtagctct
    30541 ttttctctca aatgatctag ttccatcaac tgctcttgct ggaaagtaaa tacctgctgg
    30601 aataactctt tcgtcaaagg tgcgatcagt ttttctagta actcatcact tcccacttga
    30661 tcccccagcc acaccttcga ttttccgcga ttttgctgct tgtagcgctc gacagcaaat
    30721 tcaccatata caggatgttt aagccatatt ttgccgccat aggctgagcc gtcttttggt
    30781 gtataatcct tttttttacg gccttttgaa gggaaaccaa ataatattgc ttgaataaat
    30841 tgataaatcg ttgattttcc tgcttcgttt cccccataga tcagctgatt gcctgaataa
    30901 aagtcaaaac ttttttgacg ccattttcca aaagcagcaa tctcgatcct taaaatcttc
    30961 attcctgatc ctcctcaaat aaaaaatctt gattaagcag ctgcttggct ttttcaagtg
    31021 tttcttcttg atattccggc agttcgctca atattctagc cgcttcttga tgactatata
    31081 cttcatttaa aatttgttgg aaatcttgag gtgtctgata gtattgaaat aactgttcca
    31141 tcaatttagc agaagcagca agtggggtct tcgtataaaa agtattctct atgagagaaa
    31201 tacgccaaat aaagaaatca tttatcctat ctgagaattc ctctaacaaa tagtctaata
    31261 gctctcctga ttgtatccgt tccaaaacat ctgtattgag atggtcataa tcttttaatt
    31321 ttatctcaat taaactaaat ccatcctcta cttttgaaag ctgctgacga atgtgaacaa
    31381 tgacctcttt tgtcgttctt gctttcctca atgatatttc ctttgtcttc caatagacct
    31441 ctgctacttt aatcggatgt atttggcaac ttcctttact taattcaact agtagaatag
    31501 atgtagaagc ttgttccttt ttagtatgac cttgaggcgc acctggataa acaattgttt
    31561 gcttttcatt taagaccgtt ggcacatgga tatgccctaa agcccaataa tcgtatccct
    31621 tttcttgcat ctttgagggt tggaaaggag catagtttcc tttttgagag acgccaggtt
    31681 ccccgtggta caacccaata tggtaatctg tgagttctct agaaggaaat tccatcactt
    31741 tgtctttttg aatccacggt tgacgataac taaatccact gagagagaca gtttctccac
    31801 tttttatcgt tatttttttt gtctctaccg tttcggatgt aaataaatgg acatttttag
    31861 gaaaggagaa ccaataccgc tctttttgat agaaatcatg atttccgaat attataaata
    31921 caggtatatg gttcttttct aaccgtttca tctgttctga aaaatgtttc tgtattttta
    31981 atgaaggacg attttgatga aaattatctc ctgcaagtaa aacaaaatca acgttattta
    32041 ttattgcctg ttcaatgata tttgataaaa cggtcagatt cgtcttcaat agtttttcct
    32101 gtacagtttt atcaagagtt gtcagtcctt caaatgaccg atccatatgt aaatctgccg
    32161 catggataaa tttcaccatt aaaaatttcc tccttttttt cttttatcat accattttca
    32221 atgttttttt tcatcaataa ccgaaaattt gttcgtatta tgcaaagaaa ttacgataaa
    32281 tggccgactt caaaaaataa gtttattcga aaaaaagatt gtgacatgat ttttgtcaca
    32341 atcctaaata tctatgttat attcgcttcg tttgacactg aagaagtact atttgtactg
    32401 gaatttatgc tccatttttc cgtatttaga attctatgtt ctatactacc ttaattagtt
    32461 tatatggtaa gaattctttc aaatgcttga gatactccgt ctttatcatt tgaaacagtg
    32521 acataatcag cttttttcat gatttcttga tgactatttc ccattgcaaa actaatatct
    32581 gcactttcaa acatagataa gtcatttaaa ctatctccga tcgcgattct caaaggtttc
    32641 tttaaattgt agattgattc caaacgatca aacaccacac ctttagatac attttgaggt
    32701 acgatttcaa tatttacagg tgccgattca gtaacagaca catttagcat ctttttcaaa
    32761 tggttaaata tattagcttt caattgacga ttaggaataa aaataaaaaa tttgtaaatg
    32821 ctgatagcat ccttttctat aatttcctca atattattat aagggattga acaaatagat
    32881 ttttcatact caagctcata gtttagctct ttgatagaaa attcactccc tacctcgtga
    32941 aaaatttttt tcattttatt tttataattc tgagattcat aacttccgag atttgtatat
    33001 attttggttg gaaatttcga taggacagaa tcaatttttt ttacatcatt tttatcgaag
    33061 gaagactcaa atatcttttt ttctccatca aatccaacag aaccatttaa ccctactgct
    33121 ggtatcctta aaccagattt tttgagaata ttattaatgt cttctacttc tcttccagaa
    33181 caaatgaatg gaaaacagtc ttccggtaaa tgtttcattg ttcttgcatt tttttgacta
    33241 atgctctggt cagaattcaa aagagtaccg tctaaatcta taccaattag tttcatttca
    33301 tccatgtctc ctatacgttg attataaatt cactgcttat tgtaagtcta aatttctcac
    33361 aaaacatata ctaattatac ctaatttaag taagtacaaa catcatttta agaataggaa
    33421 aggacattta tatactattc tctgtatttt tgatttataa ataaatatga attgaatcca
    33481 aatcagctat attactattt tataataacc aactataaaa aacgaagtta ggtgaaaaac
    33541 taacttcgat attcataaaa gactctatta gttcaactta gcaaaagatt taaacaaaaa
    33601 aagatgaact atttctagtt catctttttg cctt
//
LOCUS       DAPBBG010000028        33614 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPBBG010000028 DAPBBG010000000
VERSION     DAPBBG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.028"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            144..542
                     /locus_tag="RD477_001530"
     CDS             144..542
                     /locus_tag="RD477_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDT7633892.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="RD477_001531"
     CDS             526..1221
                     /locus_tag="RD477_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDT7633893.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGTDYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="RD477_001532"
     CDS             complement(1815..2702)
                     /locus_tag="RD477_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDT7633894.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="RD477_001533"
     CDS             2887..3726
                     /locus_tag="RD477_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7633895.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4815
                     /gene="sgrA"
                     /locus_tag="RD477_001534"
     CDS             3922..4815
                     /gene="sgrA"
                     /locus_tag="RD477_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDT7633896.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            5113..5469
                     /locus_tag="RD477_001535"
     CDS             5113..5469
                     /locus_tag="RD477_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633897.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5472..6629
                     /locus_tag="RD477_001536"
     CDS             5472..6629
                     /locus_tag="RD477_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633898.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6655..7095)
                     /locus_tag="RD477_001537"
     CDS             complement(6655..7095)
                     /locus_tag="RD477_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDT7633899.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7255..7761
                     /locus_tag="RD477_001538"
     CDS             7255..7761
                     /locus_tag="RD477_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7633900.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7929..9842
                     /locus_tag="RD477_001539"
     CDS             7929..9842
                     /locus_tag="RD477_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDT7633901.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9847..10848
                     /locus_tag="RD477_001540"
     CDS             9847..10848
                     /locus_tag="RD477_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7633902.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11050..11256)
                     /locus_tag="RD477_001541"
     CDS             complement(11050..11256)
                     /locus_tag="RD477_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDT7633903.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11902..12750)
                     /locus_tag="RD477_001542"
     CDS             complement(11902..12750)
                     /locus_tag="RD477_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7633904.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12749..13816
                     /gene="gnd"
                     /locus_tag="RD477_001543"
     CDS             12749..13816
                     /gene="gnd"
                     /locus_tag="RD477_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDT7633905.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13818..14819
                     /locus_tag="RD477_001544"
     CDS             13818..14819
                     /locus_tag="RD477_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDT7633906.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14816..15100
                     /locus_tag="RD477_001545"
     CDS             14816..15100
                     /locus_tag="RD477_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDT7633907.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15133..15930
                     /locus_tag="RD477_001546"
     CDS             15133..15930
                     /locus_tag="RD477_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7633908.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15944..16777
                     /locus_tag="RD477_001547"
     CDS             15944..16777
                     /locus_tag="RD477_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7633909.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16795..17652
                     /locus_tag="RD477_001548"
     CDS             16795..17652
                     /locus_tag="RD477_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7633910.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17674..18183
                     /locus_tag="RD477_001549"
     CDS             17674..18183
                     /locus_tag="RD477_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7633911.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18220..18621
                     /locus_tag="RD477_001550"
     CDS             18220..18621
                     /locus_tag="RD477_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633912.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18733..19086
                     /locus_tag="RD477_001551"
     CDS             18733..19086
                     /locus_tag="RD477_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633913.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19120..19563
                     /locus_tag="RD477_001552"
     CDS             19120..19563
                     /locus_tag="RD477_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7633914.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19580..20665
                     /gene="uxuA"
                     /locus_tag="RD477_001553"
     CDS             19580..20665
                     /gene="uxuA"
                     /locus_tag="RD477_001553"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDT7633915.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20658..21371
                     /locus_tag="RD477_001554"
     CDS             20658..21371
                     /locus_tag="RD477_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7633916.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21578..21727)
                     /locus_tag="RD477_001555"
     CDS             complement(21578..21727)
                     /locus_tag="RD477_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633917.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22123..22323
                     /locus_tag="RD477_001556"
     CDS             22123..22323
                     /locus_tag="RD477_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7633918.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            23019..23453
                     /locus_tag="RD477_001557"
     CDS             23019..23453
                     /locus_tag="RD477_001557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633919.1"
                     /translation="MIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGND
                     HSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAV
                     LLNHVRKCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(23514..23879)
                     /locus_tag="RD477_001558"
     CDS             complement(23514..23879)
                     /locus_tag="RD477_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633920.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(23955..24200)
                     /locus_tag="RD477_001559"
     CDS             complement(23955..24200)
                     /locus_tag="RD477_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7633921.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(24270..24524)
                     /locus_tag="RD477_001560"
     CDS             complement(24270..24524)
                     /locus_tag="RD477_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633922.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(24688..25011)
                     /locus_tag="RD477_001561"
     CDS             complement(24688..25011)
                     /locus_tag="RD477_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633923.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            25198..25271
                     /locus_tag="RD477_001562"
     tRNA            25198..25271
                     /locus_tag="RD477_001562"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25232..25234,aa:Ile,seq:cat)
     gene            complement(25656..26153)
                     /locus_tag="RD477_001563"
     CDS             complement(25656..26153)
                     /locus_tag="RD477_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7633924.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(26193..27002)
                     /locus_tag="RD477_001564"
     CDS             complement(26193..27002)
                     /locus_tag="RD477_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7633925.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            27336..28193
                     /locus_tag="RD477_001565"
     CDS             27336..28193
                     /locus_tag="RD477_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDT7633926.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            28186..29088
                     /locus_tag="RD477_001566"
     CDS             28186..29088
                     /locus_tag="RD477_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDT7633927.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            29121..29471
                     /locus_tag="RD477_001567"
     CDS             29121..29471
                     /locus_tag="RD477_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7633928.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            29520..30443
                     /locus_tag="RD477_001568"
     CDS             29520..30443
                     /locus_tag="RD477_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7633929.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            30521..31414
                     /locus_tag="RD477_001569"
     CDS             30521..31414
                     /locus_tag="RD477_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7633930.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            31452..32015
                     /gene="amaP"
                     /locus_tag="RD477_001570"
     CDS             31452..32015
                     /gene="amaP"
                     /locus_tag="RD477_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDT7633931.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            32029..32217
                     /locus_tag="RD477_001571"
     CDS             32029..32217
                     /locus_tag="RD477_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDT7633932.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            32230..33132
                     /locus_tag="RD477_001572"
     CDS             32230..33132
                     /locus_tag="RD477_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7633933.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            33164..33406
                     /locus_tag="RD477_001573"
     CDS             33164..33406
                     /locus_tag="RD477_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7633934.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT        11679 a         5347 c         6754 g         9834 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 acagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttcccctt catttgcttc tttttttgct
     2221 ttatctgctt cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa tgaacttgat
     4561 ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa cctaaaaagt
     4621 gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa agcgaaagaa
     4681 acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa caagctaatt
     4741 acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg ttctctcttc
     4801 catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac gatagatttt
     4861 tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc tttgccagat
     4921 ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca aatggaaaat
     4981 aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa aaatcagtaa
     5041 cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa tagataaaaa
     5101 ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt atatcaattt
     5161 ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat cgctttagat
     5221 ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc gttttgcgaa
     5281 aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa agtcgagttt
     5341 tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac gattggttat
     5401 attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct tagtcaaaaa
     5461 attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact attttgaaaa
     5521 actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa gaataccgta
     5581 tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat tctgatatat
     5641 tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat gcgccatata
     5701 cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg caacgaatga
     5761 aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg catccattga
     5821 taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca aacttctggt
     5881 cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt ggtgtaatga
     5941 acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa agttggggct
     6001 ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt tttacaactt
     6061 cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca agggtttttg
     6121 ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat tttcagcata
     6181 tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct cgcagaattc
     6241 cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac tgtatgttag
     6301 tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa tcaaagtatg
     6361 gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa cgcccaataa
     6421 aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat cctatcgaca
     6481 aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa cctacaaaaa
     6541 atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa aatagatcca
     6601 tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc aaaattaaga
     6661 cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca agagttcgtt
     6721 gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt cgctaaatcc
     6781 agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag aaggggatgt
     6841 acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa caagattttt
     6901 ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca attttagtaa
     6961 gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga taaacggtat
     7021 atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag aacctgtata
     7081 caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat aacatgaaga
     7141 aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac gtataatgga
     7201 actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac aagtatgtca
     7261 aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt tgattcttcg
     7321 ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa aacatacgaa
     7381 acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt gccacaaaca
     7441 aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt tgtgagaaaa
     7501 acccatgcga attcatttta taagactaat ttgagtgttt tattaactga attggactgt
     7561 cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac tgtgaagttt
     7621 gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac ttatgataat
     7681 ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga taaacgattt
     7741 ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat ggatactggt
     7801 ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt tttatgggat
     7861 aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc ctagaaacat
     7921 aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg gtcatcttat
     7981 ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt tcttccgaat
     8041 cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa acgaaatcga
     8101 gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt actagcacag
     8161 atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca gctacaaaaa
     8221 tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg acagaaaatg
     8281 atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac ttcggtaaac
     8341 agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta atagagttat
     8401 tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc aaacatctga
     8461 ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg atgtcgatgc
     8521 ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat ccaaatacaa
     8581 aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat gtatatgcat
     8641 attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt cgagggaatt
     8701 ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg tataatttat
     8761 tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc tatggacaat
     8821 atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg ccatctcaaa
     8881 agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta ttagcgaaag
     8941 atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca attgatgaac
     9001 aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc ataaaaaatc
     9061 agttagacat cattggggat caagagcatg aagatttatt tatgggcctt ttgaaacaat
     9121 actattttga tcaggagatg catttggata caaaagctgc tctacctacg attggaatgt
     9181 cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta cctgtgtcta
     9241 acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta gtagaaggag
     9301 cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat gtaacggact
     9361 ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa aacaataaaa
     9421 gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa aattatatga
     9481 cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg actgaaggga
     9541 tttttaatga gacgatacca actattattt tctattacaa aaaaatccca gtagctccac
     9601 caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa cctcgagagg
     9661 atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa aaaaagacag
     9721 atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt ctcatttatt
     9781 caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga aagagagggt
     9841 aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat cgtagttatt
     9901 ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt gcttgttaga
     9961 aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga tttccagcaa
    10021 aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta caaaaataat
    10081 aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct tttctggatg
    10141 catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt ggaatatcta
    10201 tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc ggaagcaaaa
    10261 catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa agagataaaa
    10321 gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc acaaattagt
    10381 ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca tcttgatccg
    10441 actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt ggatccttct
    10501 gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg tggatgggct
    10561 tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc cttggtcaga
    10621 catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa tacgtttacg
    10681 aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt cacaaaagta
    10741 gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt cagcaataaa
    10801 caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag tcttatgatg
    10861 caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt caataaacaa
    10921 gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat ggcaaggggc
    10981 accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct tttacgtaaa
    11041 ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc cgaagcctgc
    11101 ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt ccactaatat
    11161 tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa tcaatgcact
    11221 agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct tttttataat
    11281 tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat gtgaataaaa
    11341 tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat gattcagcaa
    11401 tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa tatgggaata
    11461 taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga tgtatcctaa
    11521 acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa atgataacct
    11581 aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa atttttttta
    11641 attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat tttttactac
    11701 tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa tacaaccttc
    11761 tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg taccaccctt
    11821 gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt ttatggattt
    11881 ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc gaactttagc
    11941 gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata gagagtccaa
    12001 aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg tttcttcaga
    12061 aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt ggctagtaat
    12121 aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg tttcttttga
    12181 ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag aagcttccat
    12241 caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca aaaatttatg
    12301 ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga cacgtttaga
    12361 ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact tttttgtgtc
    12421 atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct cagtatctgt
    12481 tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga tcaacatggc
    12541 cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa aaaaagtaga
    12601 gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta ctttgcttgg
    12661 attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt tgtacattgt
    12721 tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata ataagattat
    12781 cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa atcaaacaaa
    12841 aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg gtataagaaa
    12901 tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc
    12961 tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt
    13021 tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac
    13081 ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat
    13141 gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc
    13201 ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt
    13261 ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg
    13321 aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag
    13381 aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg
    13441 aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga
    13501 gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg
    13561 ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg
    13621 gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc
    13681 tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga
    13741 tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt
    13801 tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg
    13861 gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta
    13921 ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca
    13981 ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc
    14041 tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac
    14101 accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta gcagaaaata
    14161 atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg
    14221 acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg
    14281 gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc
    14341 gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg
    14401 caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga
    14461 atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa
    14521 atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg
    14581 gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac
    14641 gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct
    14701 acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca
    14761 gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat
    14821 gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga
    14881 aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt
    14941 cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga
    15001 aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg
    15061 aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa
    15121 ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct
    15181 actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga
    15241 caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc
    15301 caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca
    15361 aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg
    15421 aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa
    15481 gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt
    15541 atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag aaatgcggca
    15601 gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat
    15661 ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa
    15721 atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg
    15781 ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa
    15841 gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg
    15901 gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt
    15961 agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg
    16021 aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat
    16081 catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg
    16141 ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt
    16201 accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt
    16261 cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa
    16321 atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct
    16381 ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt
    16441 aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat
    16501 tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat
    16561 cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa
    16621 atctttagat attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt
    16681 tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc
    16741 ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct
    16801 gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa
    16861 gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg
    16921 ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct
    16981 ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc
    17041 ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa
    17101 gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg
    17161 gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca
    17221 atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc
    17281 tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc
    17341 cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga aatgctatca
    17401 gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca
    17461 ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg
    17521 ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga
    17581 ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc
    17641 ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg
    17701 gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat
    17761 tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa
    17821 aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc
    17881 tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa
    17941 acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt
    18001 gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca
    18061 gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa
    18121 gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca
    18181 taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt
    18241 cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa
    18301 cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc
    18361 attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa
    18421 gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc
    18481 atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt
    18541 ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta
    18601 ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag ctactttttc
    18661 ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca
    18721 tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg
    18781 tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa
    18841 aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct acagttttcg
    18901 ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa
    18961 aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa
    19021 acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt
    19081 cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt
    19141 acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg
    19201 agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag
    19261 gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta
    19321 attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag
    19381 aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat
    19441 catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa
    19501 gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt
    19561 tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc
    19621 gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct
    19681 gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt
    19741 tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa
    19801 ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt
    19861 gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa
    19921 aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat
    19981 cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt
    20041 caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta
    20101 tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat
    20161 tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga
    20221 actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc
    20281 agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga
    20341 aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag
    20401 aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg
    20461 ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat
    20521 acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta
    20581 tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa
    20641 accactggga gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt
    20701 tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt
    20761 atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag
    20821 aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca
    20881 atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag
    20941 aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata
    21001 cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa
    21061 tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt
    21121 gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga
    21181 tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt
    21241 tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata
    21301 aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt
    21361 tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg
    21421 agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga
    21481 acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga
    21541 gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt
    21601 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
    21661 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
    21721 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
    21781 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa
    21841 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
    21901 tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc
    21961 attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg
    22021 taatttgagc gatatatcta catagataaa actccttttg cctatcaaat atttattgca
    22081 acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta
    22141 aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta
    22201 tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca
    22261 gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct
    22321 taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa
    22381 aattcattgg tcgttgaatg ggctgaatag tagtgtataa aacaattttg tggagcgtga
    22441 agcttttatg gtagaggaaa atacgaataa agtgatgttg ataaaaagat tcctatcttg
    22501 aatatgaatc gagatgtctt gaaaatgaat gagtttgacc aatcattatc ctatcgaaat
    22561 ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa tatgttctag
    22621 gaagagggcg gcacatctaa atttttaagt aacggacagt tcaaatatca agcattgaaa
    22681 gaggctatcc tactgtttga aggaatacga atatataaaa acgacaaaga ggaaaaagta
    22741 gttcattaaa tcccttcaag tgatgaaaga atgagatcta ataaaacctg atctttcatc
    22801 tgttttttta ataacgtaat aattttttta ttttttgtgc tttcgccgtt attgtagagt
    22861 gatttagcat tcttattttt aaaaataact gtgttatata ttaaaaaagg cataatagtt
    22921 ataagctttt ttaaagaata tttagaaatt attaattttc gtctgaaaac aaaaaaagaa
    22981 agcgttatta tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
    23041 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
    23101 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
    23161 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
    23221 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
    23281 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
    23341 ggaaatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
    23401 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
    23461 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
    23521 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
    23581 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
    23641 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
    23701 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
    23761 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
    23821 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
    23881 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
    23941 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
    24001 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
    24061 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
    24121 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
    24181 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
    24241 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
    24301 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
    24361 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
    24421 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
    24481 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
    24541 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
    24601 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
    24661 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
    24721 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
    24781 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
    24841 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
    24901 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
    24961 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
    25021 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
    25081 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
    25141 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta
    25201 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
    25261 tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
    25321 gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat gcaagctttt
    25381 gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat tagcttaaca
    25441 aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag ggtcagctga
    25501 ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt cataaaaaag
    25561 ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg ctttttttat
    25621 tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat agagacgttg
    25681 aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac taacattgga
    25741 tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt agagagtaca
    25801 cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt gctttcaaat
    25861 aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata ctttttaatc
    25921 actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc tcgttcttct
    25981 tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc attattttgg
    26041 cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc tggaatataa
    26101 aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa cattttcttt
    26161 cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa aatataagca
    26221 acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac ttgtcgctgt
    26281 gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc gttttcttga
    26341 tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag aaaaacaagt
    26401 gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc agagaatact
    26461 gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc ttcactctta
    26521 tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt taaaatttga
    26581 tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt gactagattt
    26641 gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat ggatattttc
    26701 gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt tgaacgtctg
    26761 agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt ggaagaaatc
    26821 aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct gttccttccg
    26881 gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg gatattttct
    26941 tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc
    27001 attttatctt cctactttct ttttacatat ctattttcct gttaccttaa gattaaatga
    27061 gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa aaaacattct
    27121 taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata aaaagccatg
    27181 aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt aaaatattca
    27241 atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta gatgtagaac
    27301 aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac ggcgtagttc
    27361 ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg atcgcaactg
    27421 cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac gatggtctat
    27481 cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt aatgcaagta
    27541 tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt gaccatatca
    27601 caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag gattataaga
    27661 aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa ctatatgatg
    27721 agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg aaagctttgg
    27781 aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg gtgattgaca
    27841 ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc agtgaaaatg
    27901 gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat gaagattggg
    27961 aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt catccagcac
    28021 caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg atcgttcact
    28081 ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc aatttatact
    28141 taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa taaaaaagaa
    28201 atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc attatttgtc
    28261 tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat tgacgatgat
    28321 atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg aatgaactca
    28381 gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat cagcgatcgt
    28441 attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg cacagaagtt
    28501 gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc taaattatgg
    28561 cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt ccatcaacga
    28621 ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg tttgatgctg
    28681 atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga tttcatcgaa
    28741 cataatccag aaaaattacg attccatgac caagatgcat taaatgctat cttacatgac
    28801 cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc aaaagcaaaa
    28861 aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt
    28921 atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa
    28981 tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt ccctaaatat
    29041 ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata gaagacaaca
    29101 actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta gagacgttaa
    29161 ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat tccgatccat
    29221 ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt
    29281 cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc tatacatcta
    29341 aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca
    29401 gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta aagaactgct
    29461 tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga tactactttg
    29521 tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg ctcgtacttg
    29581 atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt cctgatgata
    29641 ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt tatgagacga
    29701 aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat aatcgtatcg
    29761 aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg
    29821 atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt
    29881 ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg aaagaactaa
    29941 gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg
    30001 aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa
    30061 gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag
    30121 acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg
    30181 agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg atggacaata
    30241 atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt
    30301 tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag
    30361 gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat
    30421 accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt
    30481 gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat
    30541 cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc
    30601 tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa
    30661 tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc
    30721 agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga
    30781 aaaagatgca gaggaagtag caaaatacgt gaaagatgca ggacgtaaga ttgtcttact
    30841 accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga
    30901 acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat
    30961 tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt
    31021 tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac
    31081 ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc
    31141 aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa
    31201 tggtgtagca cctggtccaa tctggacacc attacaacta gacgaaggac aaccttctga
    31261 ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt
    31321 agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta
    31381 cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag
    31441 gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc
    31501 tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag
    31561 agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg
    31621 ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa
    31681 caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa
    31741 ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa
    31801 atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt
    31861 cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca
    31921 tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag
    31981 ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta
    32041 ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta
    32101 tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc
    32161 gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag
    32221 gaaacatcca tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca
    32281 gtgtctgcac atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag
    32341 aacaaaacag caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga
    32401 gtagaagaga agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa
    32461 tctaaagttc cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa
    32521 catccttctg accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa
    32581 ggaaaacttg gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat
    32641 tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca
    32701 attgaacaag tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga
    32761 aaacttgtga atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa
    32821 gtagcagttg atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa
    32881 gaaatgcaag aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa
    32941 gtcaacgcta acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg
    33001 gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca
    33061 gataaagtaa aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca
    33121 cgggttcagt aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc
    33181 acttatcgtc ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc
    33241 gatggggatt attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt
    33301 attaggttct tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc
    33361 catcatttta gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa
    33421 agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt
    33481 ctcatatttt tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta
    33541 tacggaataa gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt
    33601 cccaattaga tttt
//
LOCUS       DAPBBG010000029        33087 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPBBG010000029 DAPBBG010000000
VERSION     DAPBBG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.029"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            253..483
                     /locus_tag="RD477_001574"
     CDS             253..483
                     /locus_tag="RD477_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7633935.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL"
     gene            483..824
                     /locus_tag="RD477_001575"
     CDS             483..824
                     /locus_tag="RD477_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7633936.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQLKSLDFKERRLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            1519..3600
                     /locus_tag="RD477_001576"
     CDS             1519..3600
                     /locus_tag="RD477_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7633937.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAERLAAEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFSLKLENDKQVRLETEIKEVKDGFLNRRERIFDLVLAIAYS
                     RNPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFFHYFPLAEIKKIEEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDYMITEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHIPFHLKEEALFENLYKHMAA
                     LIIRIKHAIQVMNPLKENIRTNYFPIHRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYQSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVDLVFSTFQLNYRNKPLLVIDPILTETNILVIEDFLKRNEELQRLKPNSHESTQLFT
                     QIIGVMEESGGRVDRIIYGKLEKLFEINNLEINKREIQPMLKDILTDSNIMIKEKAAT
                     WEETIERAAKPLVKENIVEERYVEAMIRAVNEYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NVEDFKQLLFQ"
     gene            3636..3938
                     /locus_tag="RD477_001577"
     CDS             3636..3938
                     /locus_tag="RD477_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7633938.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFSVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            3964..5253
                     /locus_tag="RD477_001578"
     CDS             3964..5253
                     /locus_tag="RD477_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7633939.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEEIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPILKAF"
     gene            5272..5679
                     /locus_tag="RD477_001579"
     CDS             5272..5679
                     /locus_tag="RD477_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDT7633940.1"
                     /translation="MFGFFKKKENEPKKRSIKEEKILSPEVTEKIQSEITQLNQEIST
                     TQEKPKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDVMRQIAKQVTIKGNK"
     gene            5702..6571
                     /locus_tag="RD477_001580"
     CDS             5702..6571
                     /locus_tag="RD477_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDT7633941.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKGTFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            6573..7193
                     /locus_tag="RD477_001581"
     CDS             6573..7193
                     /locus_tag="RD477_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7633942.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SVGFNNLEERAKQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKTRLQELAERY
                     GYQVMTIRLTADFEVLWQRRYKRDREPDRHLSYIMDHYHYGDILEDRSKGTNHITKEA
                     FKKIITDRKYDEFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            7390..7995
                     /locus_tag="RD477_001582"
     CDS             7390..7995
                     /locus_tag="RD477_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7633943.1"
                     /translation="MNNKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTVIEEGHPTPKVDVRAYIRKDGKILLIEDKRTREWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVESTQLQAVFDTNKQKDIPQLFQYYKLVFACTIHDEDAKFI
                     ENNETSDMGFFSIEELPKLSEKRTTKKQLLILENKNQLYCD"
     gene            8131..8583
                     /locus_tag="RD477_001583"
     CDS             8131..8583
                     /locus_tag="RD477_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7633944.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLKKVCKNVSEDWNFVKKGNKRIY"
     gene            8620..9116
                     /locus_tag="RD477_001584"
                     /pseudo
     CDS             8620..9116
                     /locus_tag="RD477_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9134..9844
                     /locus_tag="RD477_001585"
     CDS             9134..9844
                     /locus_tag="RD477_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633945.1"
                     /translation="MKKVIVDANFWEVFPEATIEILSVSGIDNHVTEENEEMYHQLLN
                     SAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKISGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVSIVRHTLTKDESEAEL"
     gene            9986..10408
                     /locus_tag="RD477_001586"
     CDS             9986..10408
                     /locus_tag="RD477_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDT7633946.1"
                     /translation="MESVYRQERKSTRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGIFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMDEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            10428..10652
                     /locus_tag="RD477_001587"
     CDS             10428..10652
                     /locus_tag="RD477_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633947.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRARNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKVKSHY"
     gene            10896..12209
                     /locus_tag="RD477_001588"
     CDS             10896..12209
                     /locus_tag="RD477_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDT7633948.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALSGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAMLLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGKLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            12537..14174
                     /locus_tag="RD477_001589"
     CDS             12537..14174
                     /locus_tag="RD477_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDT7633949.1"
                     /translation="MERKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDVIWLSPVYESPNDDNGYDISDYQAIMAEFGTMEEMEELIEEAKKRNIRIVMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGEEPNGLRSIFSGPAWQLDEQTGQYFL
                     HLYSKRQPDLNWKNKQVRQAIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSDPKRKELSMIFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDLYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDISEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEETPEHVFAYSRIYEDQRWLVVCNMSSEKQVFTVPRE
                     ALRIIVSNYPLKQLPDGKVELRPYEAFVMEVNKEKLI"
     gene            14322..14756
                     /locus_tag="RD477_001590"
     CDS             14322..14756
                     /locus_tag="RD477_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDT7633950.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRLLNKLADEAFRKTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FTKEVDGSWEELHQSYKAVLGNEASKELAEIMNEATDKLQKK"
     gene            14859..15950
                     /locus_tag="RD477_001591"
     CDS             14859..15950
                     /locus_tag="RD477_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDT7633951.1"
                     /translation="MSQFDQSIILPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIVQIF
                     HGGRMTSRATLSGEQPVSASAVAAERPDAETPRALSENEIKETIQAFGEATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIDELLTTVFSTVEKYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRETKLDYLHVSLNAYDRRARSDKYSDQSI
                     LEAVHDTIQGKIPLIGVGGIRTRKDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            15980..16657
                     /locus_tag="RD477_001592"
     CDS             15980..16657
                     /locus_tag="RD477_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDT7633952.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYKDEDFRNRALGNTLRPDDVDAKDYAAVYYAGGH
                     GTMWDFPEATDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGADYQSGPAFSEVVQIDGRLLTGQNPQSAHQLGEV
                     VVEALKH"
     gene            17002..18297
                     /locus_tag="RD477_001593"
     CDS             17002..18297
                     /locus_tag="RD477_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7633953.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLIPTSEIYLIEKQSKLGWIPSGF
                     NLIVKGKVTSEKQLSWITKEELIDRYRIHVYTEKTVIGLEEKKVVLAGGDKLDFDRLI
                     LATGSNQNFRNVPAEARFIHPVKKIQNIAALQQKIMDAKKIAIIGAGQAGIEIAEGLA
                     SGKTQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGISLFLEEQVVSLSENETAVVET
                     ERRRENYDLVLLANHSRPNNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSATKDQGTYRPVGNQWFGYFLGSVGMTEE
                     ESIFYPQKIETGYAEYKLAAINQSKVKVKFLFDSEKKILGAQLQSRKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPNMVWSKVANTAYED"
     gene            18287..19747
                     /locus_tag="RD477_001594"
     CDS             18287..19747
                     /locus_tag="RD477_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7633954.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKTSF
                     DQYLEDIELIGRMMGKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLGRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYETLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLEKLQETAMQQLPRKSTEDSALSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLNRELRGVSGIEIESYEEGKNYDLVITF
                     CQRNKQQNEYYLSEFASPYDIIRLKRRIEMLKKEKN"
     gene            19884..21380
                     /gene="glpK"
                     /locus_tag="RD477_001595"
     CDS             19884..21380
                     /gene="glpK"
                     /locus_tag="RD477_001595"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDT7633955.1"
                     /translation="MTEPTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQDILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEAAA
                     KRSTNEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            21394..23217
                     /gene="glpO"
                     /locus_tag="RD477_001596"
     CDS             21394..23217
                     /gene="glpO"
                     /locus_tag="RD477_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDT7633956.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQAAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQGIAPHIPKPDPM
                     LLPIYEDEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYEGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LADIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVRELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMTPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAEYFGWTEEEKVQQTKRLEALIAESDLRELKGEK"
     gene            23218..23934
                     /locus_tag="RD477_001597"
     CDS             23218..23934
                     /locus_tag="RD477_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDT7633957.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            24240..26168
                     /locus_tag="RD477_001598"
     CDS             24240..26168
                     /locus_tag="RD477_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDT7633958.1"
                     /translation="MNNEMISQLQFDQIKKEIQARAIGNYSKKRISKMTTSSNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLVLSPADLIEYADFLRSSRMI
                     TKFFDKNQYQAPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQII
                     EKEIQSKLLKFLRHPKNKEMIQEAIIVQKGECYTIPIKASYKNKVDGTIIDESNKGTT
                     VFIEPTVVSKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEAIDLLIETMTVLDIIF
                     ARAKFSREINGITPKINKSEHIVIKQGRHPFLPDHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            26294..26848
                     /locus_tag="RD477_001599"
     CDS             26294..26848
                     /locus_tag="RD477_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDT7633959.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(27578..27892)
                     /locus_tag="RD477_001600"
     CDS             complement(27578..27892)
                     /locus_tag="RD477_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDT7633960.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(27906..28166)
                     /locus_tag="RD477_001601"
     CDS             complement(27906..28166)
                     /locus_tag="RD477_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDT7633961.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            28336..28650
                     /locus_tag="RD477_001602"
     CDS             28336..28650
                     /locus_tag="RD477_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7633962.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            28652..30307
                     /locus_tag="RD477_001603"
     CDS             28652..30307
                     /locus_tag="RD477_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7633963.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            30390..32525
                     /locus_tag="RD477_001604"
     CDS             30390..32525
                     /locus_tag="RD477_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7633964.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            32700..33065
                     /locus_tag="RD477_001605"
     CDS             32700..33065
                     /locus_tag="RD477_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633965.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
BASE COUNT        11112 a         5608 c         7131 g         9236 t
ORIGIN      
        1 agatgaacta gcaagtttga ttgcagactg tagacaaagg tatcccttgc ctctttacga
       61 agagtgtagg ttgttacttt tgtctatttt tgtatagaaa tagaaaacat ttgtctccat
      121 ttccaagctg ctaatttttc aaagtgcacc atttctcaaa atgcttttga tcatttctcc
      181 atctagttta caaagggtac ttttgcgtta cttagtgttg tgtagtatac ttttactgaa
      241 gaggtgatga tgatgcttgt tacaaaatct agattacaag gaagttctgt agtggttact
      301 ttaccttctg ataacggaaa gaaaccttca gaaaaccaag aatatatcgt tgtatattct
      361 gacgatggga caattacgtt agttccaaaa attgaagatc catttagtgg tggagaagag
      421 gccgaatatt atgaaaaaga cgaatgggaa gatttaactc ctgaagggag agaaaggtta
      481 tagtgtcaga agaaagaatt tatattccta aaaaaggtga tattgtgtgg attgattttg
      541 atccttcgat aggaaaagaa attcaaaaaa gacgaccagg actagtcgtc tctcgttatg
      601 agtttaatcg taaaacaatg ttttcggtca tatgtccgat tacttccacg ataaagaatt
      661 taccgacacg ttactcatta ccagaagagc tagatataaa aggacaagta cttatttctc
      721 aattaaaatc acttgatttt aaggaacgaa gactcaaaaa aatagaaaac ctgccattgc
      781 aagacatggc taaaattgat cagattatac aatatatgtt ttaagcgttg taactaaact
      841 ctacaatatt tttatatgct aaacttaaca aaattttttg acatttaaaa agaaaagtga
      901 caataaaaaa tagcatctaa atagcaatga cccccaaatt taactcttgg ggggacattt
      961 caaaagggat aagaaaacaa acattctcgt cgtatctgac tagtgaaaaa gatggcaggc
     1021 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaatg gagctgtgac
     1081 aaaaattttg tcgcagctcc tttttctgaa taaacggtgt tccagagata gcctatcggc
     1141 aataaggcac gattttgtaa aatatgggaa gctatttttc aaaatcgctt cttatcgctc
     1201 aaggctgaac ctctccgtca caacctttcc actaaatggt attcaaaagc ttacatttac
     1261 ggtaataagc tcaaccaaac gaaaaatagg aagcactatt tttgtttggc tgttcttctt
     1321 acttgatgca ggacagcttt ttcatgacct cttttttgta ataataggcg tatgtctttt
     1381 tcgttgagaa gttgaaaaag catacggctt attttttgtt tgctatgctt actttctttc
     1441 cacttcaagt ggcagaaata tctacttttt attatgtaag cggaaccata tacttggaat
     1501 cagaaggaga tcatttctat aaaaacaaga acaattgaaa tacttcacag aatcatgaca
     1561 gcccaatatc cagtaaaagc ggaacgtcta gcagctgaat tcaatgtcag tcagcggacg
     1621 atccgtcagg aagttcttga agcgaatatc tggttgagta gtaaaaagct tccggaaatc
     1681 agaacaattc gcaacaaagg tttttcgttg aaactggaga atgacaagca agttcgatta
     1741 gaaactgaaa taaaagaggt gaaagacggc tttttgaatc ggcgggaacg tatttttgat
     1801 ttagttttag ccattgctta cagccgaaac ccgatccatc taaacaaaaa agaagaagaa
     1861 tttcaaatct ctaaaagtac gttagatgaa gatatgagac gattacgagc aacactggtc
     1921 agttatggaa tcgagatcgt cagttatgga aagcagggac tggtttataa aggttcagaa
     1981 cgatctatcc gtacgatgat ctatgatttt atcaatcaga atctggggat gaccgacttc
     2041 tttatgaatc aagatgcaca actaacaccg gctcaaaaaa tctttttcca ttattttcct
     2101 ttggcggaaa tcaaaaaaat agaggagatc tattccaata agttgttaaa acaagaggat
     2161 gatatttata aaaagcaaat tctgcttttt acattgatct ggatccaacg aatcaggaaa
     2221 catgaattca tctctgccat taattggaaa aatacagaaa taaaagacag cagcttctct
     2281 gcatttattg actacatgat cacggaattt caattggaca atgttccaca agtagaaaga
     2341 aattatcttc gtttcactat cgagacgttc aatgctagag atatctccaa ttcgattgag
     2401 tgggtacaag ctcaactact aacgatccaa ttgatccaat ttgtagagaa cgagacacac
     2461 ataccttttc atctaaaaga agaagcttta ttcgaaaatc tctataaaca catggctgca
     2521 ctgatcataa gaatcaagca tgctattcaa gtcatgaatc cattgaaaga aaacatccgt
     2581 acaaattatt ttccaatcca ccgcgcagtc actcattttg ttcctacgat cgaagaagta
     2641 atcggcggca agatactgga ggacgagata gcttttcttg tcatccactt ttcgacgatt
     2701 gccagtgtga tcaagcgaga cctgaattat gtttatcaat cagttgttgt ctgtaatcac
     2761 ggcatggcta ccgggaatct gttagcagaa aatctgaaag aaaaatttcc tcaaatcgag
     2821 attactgcag ttttaagttc aaaagaaatc gaattagtgg ataaactgga tgtcgatctg
     2881 gttttcagca cctttcaatt gaattatcga aataagccgc ttttagttat cgatccaatt
     2941 ttgactgaga cgaatatttt agttatagaa gactttttga aaagaaatga agaactgcag
     3001 cggttaaaac ctaacagtca tgaatcaacg cagttattca ctcaaataat cggtgtcatg
     3061 gaagaaagtg gtggccgagt agatcgcatc atctatggca agttggaaaa attatttgaa
     3121 ataaacaatc tagaaatcaa caaaagggag atccagccta tgttaaaaga tattttgacg
     3181 gacagcaata tcatgatcaa agaaaaagcc gctacatggg aggaaacgat tgaacgagct
     3241 gctaaaccat tggtcaaaga aaacattgtt gaagaacgct atgtcgaagc gatgattcgc
     3301 gcagtgaatg aatacgggcc atatatcgtg attggcaaac acatggcgct agcccatgct
     3361 cgaccagaag atggggtcaa taagttagga gtcagcgtag ctacgattga gcaaccaatc
     3421 gatttcggta atcctgaaat ggatccagta aagattattt tttgtttagc ggcagtagat
     3481 tcctactcgc atctgaacat catgaaagaa ctgatcgaac tgatcaacaa tgaagagaag
     3541 ttggatcgat tgatcgagtg cacaaatgtt gaagatttca aacaactgct ttttcagtag
     3601 ttgagaaata ataaatgata cgggggagaa caaagatgaa caaattaaac attttattcg
     3661 tatgcggcgc aggtttagga agtagttttg cagcacagat gtctgctgag gatgttttga
     3721 acaaattagg agtagaagcc aaactggacc attgtgatat ttcttctgca gtgtccatga
     3781 aaccagatgt gatcatcaca gcagaaaatt ttcgctcgca gttcgaaaaa ttctcagtcg
     3841 atcctaaaac agcgatgatc tatttgaaaa atattgtctc gaaaaatgaa attgaagaaa
     3901 aaatcacccc agttctacaa gataagggag ttatctaaat aaaaaagaaa agaggataaa
     3961 aacatgggcg ttatcaattt tatcatcgaa aatattttga ctcaggcatc gatcaccatt
     4021 gcattgattg ctatgttggg tctactgctg cagaagaaat cagcaggaca agtcatttct
     4081 ggcacgttaa agacactcct aggattccaa gtgttgagtg ccggttcgag tatcatcgtt
     4141 ggaagtttaa cttatttcgg gaaaattttt actgaaggat ttcatatgca aggaatcatt
     4201 ccttctattg agtctatcaa tggtcaagca atgaatgatt taggtttagg tagagatatt
     4261 gcactgacgt tcttagcgat ttttgtattc aatattattt tggctcgttt cacaaaatgg
     4321 aagtacattt ttctaactgg tcaagccatt ttatggatgg cgacgatgac gacggtcttt
     4381 ggttatttcg ctggtctgcg agggatcgta ttgatcttag tcggtggttt tatcggagct
     4441 tgtttcgcca ttgctatgcc agcagtagcg caaccaatta tacgtaagat tacaggatcg
     4501 aatgatattg ctttagggca tttttgtacg attggatact tatttgaagc tggcgtggct
     4561 aaactttttg gtgagaaagg tgaaaataaa aaatcgattg aagagatcaa attgcccacg
     4621 catttcgagt ttctgcaaga tacgtatttg tctgtcatgg tcgtgatggt cccactatat
     4681 atcatcactg ttttgtttgc aggtgaacca ttcgcttctg aattatcagg agatcaaaac
     4741 tatatcatgt ttgccttttt acaagccatc caattcgtag tgggtgtgta tgtattgcta
     4801 gctggcgtac gtctactgtt aggtgaaatc gtgccggcat ttcgagggat agctatgaaa
     4861 ttagttcctg atgcgattcc tgccttagat tgtccagttt tctttcccta tagcccaaat
     4921 gctgttattt taggtttcat tacgacaaca atcggtacga ttattgccat gttcactttg
     4981 ccgatgttcg gcttagcgat gattttacca ggtatgctaa cgaatttctt tgccggagga
     5041 acagcgggga tatttggtaa cgcagttggc ggacgtcgag gagcgatcat tggcggtatt
     5101 gctcatggct tctttattac attattgcct gctttgctag taacgatctt taattcgatg
     5161 ggattcatca atgctacagc aaccgatgta gatacagtag ccgccgcact cttatacgct
     5221 tggatcttaa gcccaatttt gaaagcattt taattgaaag tagggaaaat gatgttcgga
     5281 ttttttaaga aaaaagaaaa tgaaccaaaa aaaagatcga taaaagagga aaaaatcctc
     5341 tcgccagaag tgaccgaaaa gattcaatca gagattacac aattaaacca agagatttca
     5401 acgacccaag agaagcccaa attagcggaa ctttacgaac aagttggact taaattttct
     5461 gaattgtatg agaacgatca agccatccaa tatttagaaa agagcttgga aaataaacag
     5521 acgatcggag atgggtataa aaaactgatg agcctctata atcagaagcg tgcagatgct
     5581 gctcgtgcgg gtgatgatca agggatcgat tactacatgg gcaaaatgga tgtgatgcga
     5641 caaattgcaa aacaagtaac gatcaaagga aacaaataaa aagataaggt ggcaataaaa
     5701 aatgtataca acattgaaag aagtaacaaa aacagcagaa gagttgaatt ttacagttgg
     5761 agcttttaat acgcataacc tagaaatgct tcctgccatg ctaggcgcag caaaagaaat
     5821 gggttcgcca atcattattc aaacaagtgt cgacaccgca aaatatatcg gtatgcatgt
     5881 gttagtcaat gtaatgacag ctattgcaga agaagaaatg gttgatgcag tcttgcactt
     5941 agaccacgcc cgagactttg atgatatcaa agaagcaatc gatagcggat atacttctgt
     6001 catgtatgac gggtcccatc tgcctttcaa agaaaatatc ttgaagacga aagcagtggt
     6061 agaatatgcc catgcacacg gtgtttccgt agaaggagaa cttgggacga tcggcggtac
     6121 agaagaagga atccatgtcg ctgaaaatga caaagtatat acaagaccgg aagacgcagt
     6181 gaaatttgta gaagctacag gtgtggatgc attagctatt gctatcggta cgaatcatgg
     6241 acaattcaaa tcaaaaacag aagtgaacat tcccttgctg aaagaaatcg atgcagtagt
     6301 cgatgtcccc ctcgttatcc atggcggaac aggtgtgaaa gaagaagatt atcccgaatt
     6361 gatcaataac gggatccgca aattcaatgt tggaacggag cttttagtca actggacaaa
     6421 agtggctaaa ggaaccttta gtgaaacaga aatcaacaag tctcttcgcc ataatgtgat
     6481 tccagcaaat gaagcagtaa aagaaattgt caagcataag attggactgt ttatgaactt
     6541 ggaaagtcca attaacttag gaaacaaatg agatgaaaaa gtatttcatt cttctagctg
     6601 gtagtcctgg tacaggaaaa acatatctta tgaataaact acgggaacga tttccagata
     6661 tgtatgccat cacgctcgat gaaatcaagg aatattatgc agaatccgtc ggttttaaca
     6721 acttggaaga acgggcgaaa caagaaagga cgaaagttta tcctttctat tacaaagcac
     6781 ttgatctgta tatggaagca ggaaagaaag tgattgtgtc agagtatcct tttagtgata
     6841 agcaaaaaac tcgactccaa gaattagcag aaagatacgg atatcaggta atgacgattc
     6901 gcctaacagc agactttgaa gtgttatggc aacgtcggta caaacgtgat cgtgaaccag
     6961 atcgtcattt gagctatatc atggatcatt atcactatgg cgacatattg gaagatcgat
     7021 cgaaagggac gaatcatatt acgaaagaag cattcaaaaa aatcatcact gaccgaaaat
     7081 atgacgaatt tcagttagga acactatatg aatttgatgt aacagactat actcaagtag
     7141 attacactcc actattggat aaattagaaa aagaaatcaa tgacaataag taacgaagaa
     7201 atggtttgat agtgacaggt aataaaaaag acatttggtc cccagaggag ccaaatgtct
     7261 ttttgttcta aaatgttttt tcatattctt ttatttattg ccgatttcct tgtgtttatt
     7321 tgccgtaatt aggtcagtct tctatacttg attacatagt catacatttg ctgaaaagaa
     7381 gggaagagaa tgaataacaa gttggcccaa tacaaaagac tgctcgcact ttctcaagct
     7441 ggtctgtatt atggaaaaga tgtattcgat aaagaacgat ttgaagagat tcggacgatc
     7501 tcattggaat tgatcagtga aataggaaaa gaaactttcg aagaaataaa agcacttaca
     7561 gttattgaag aaggtcatcc cacaccaaaa gtcgatgttc gtgcctatat cagaaaggac
     7621 ggcaagatcc tactaatcga agataaacgg acgagagaat ggtcactgcc aggcggattt
     7681 gccgaaatag gtttgtcccc tgaagaaaac gtgcggaaag aagtctatga agaaacgggt
     7741 ttaacagtag aatctactca gctgcaagcg gtttttgata cgaataaaca aaaagatatt
     7801 ccgcagcttt ttcagtatta taagcttgtt tttgcttgta cgatccacga cgaagatgca
     7861 aaatttattg agaacaatga aacttccgac atgggatttt ttagtataga agagctgcca
     7921 aagctatcag aaaaacgaac aacaaagaag cagttgctta ttttagaaaa caaaaatcag
     7981 ctctattgcg attgagtttt ttcgtaaaag aagaaagctg tttactctgc gagttctcta
     8041 aaaaagaact cgttctttta ttaaaaaata gttgcatttg caacaaaaag aggtaaacta
     8101 gtcttacaaa caaaataaag gtggggtgaa atggctgaaa ttcttcgaga cattggaatg
     8161 attgcccgag cgttggattc tatcagcaat atcgaattca aagaagttga tttaacaaga
     8221 gggcagtatc tttatttagt acgtatttgc gaaaatccag gaatcatcca agaaaaacta
     8281 gcagaaatga tcaaagtaga tcgtacaacg actgcacgag caatcaagaa attagaatcc
     8341 aatgggatga tcgaacgttt ggaagataaa gaaaataaaa aaatcaagaa actttatccg
     8401 actaaaaaag gagcagaaat ctatcctttc atcatccgag aaaacaacta ctctaatgca
     8461 gtcgcattaa atggtctgtc tgacgaggaa gcaaaacaat tggaatatct tttaaagaaa
     8521 gtatgcaaga atgttagcga agattggaat tttgtcaaaa aaggtaataa aaggatctat
     8581 tgaaaggaag aagccgtgaa taaatacgaa attcgagaaa tgacgccttc agatgtagac
     8641 ttactacaac aaatcagtat cgaaacattt acagatacat ttggaaaaga taattcgccg
     8701 gagcatttag ctgcttactt gagacaagct tatgcgccag aaaaacttac agaagagcta
     8761 aaagagaaag aaactgattt ttactagtaa gtatggacca agagcctgcg ggttacctga
     8821 aactaaatat aggaacgagt caatctgagg aaatggatga tcatttttta gaaatcgagc
     8881 gaatctatgt acgaaaaaaa tttaaacatc aaggtattgg tacattttta ctagatacag
     8941 ctatcaaaaa agcaaaagaa aagcaaaaaa cagccatttg gctcggtgtg tgggaacaca
     9001 acgatgctgc gctcgctttc tatcgcaaaa gaggatttac caagtacagt gaacatgttt
     9061 tttatatggg agatgatccc caaacagact atctattcat caagcaatta ttctaatcaa
     9121 gaggtgtgaa catatgaaaa aagtaatagt ggatgccaat ttttgggaag tatttcctga
     9181 agcaaccatc gaaatcttat ccgtgagtgg tatcgataat cacgtaacag aagaaaatga
     9241 agaaatgtat catcaattat tgaatagcgc ggcaaaagaa gcaagaaatt accttacgga
     9301 agaaacattc agccaaaacg aagtcattgc tcagtggcgt caggctttta ccacattcaa
     9361 gactaaaaaa ggtgcacgtt cttctattga agctttactg aaacgtgcaa atcaagaacg
     9421 ggaatttttc ccaatcaatc cgcttgtaga tatttataat agtatttcct tgtcttttgg
     9481 cgtaccttgc ggcggagaag atatcgaaaa gatttcagga aatctgcatc taggaaaagc
     9541 ccaaggtggc gaatcattcc tgcctttagg tgcagattcc gatgctccgg cacttccgga
     9601 agaaatcatc tactatgatg aacaaggagc aatctgtcgc tgtctcaact ggcgtgaagc
     9661 ccaaagaacg atgctgacgg aagaaacgca agcagcggtg ctggtcattg aatccatcaa
     9721 tgctgaacaa gcggcacgcg gcgcacaggc aatcgaagcg ttgaaggaaa aaatcgatca
     9781 aacatttggc gtgtccattg tcagacacac actaaccaaa gatgaatcag aagcagaatt
     9841 ataaaagaat cgaaaaaaga agagcggcat tcattgccgt tctttttttg taaaaaaagt
     9901 ttgtggagga aagcaagtct agaaactatt gctttatttt tcttgaatag gatcaaacac
     9961 aaggtgttcg cgctttcgtg atataatgga atcagtttat agacaggaaa gaaaatcaac
    10021 tagaaagaag ggtcaaacga tgatacaaaa aacacgtgtg atgctttatg tcaatgacgt
    10081 agagatgatt tgccgattct ttgtagaaaa aattggtgca gaaatttcag aaacgatcga
    10141 attgccagaa gaatttaaga gtattgtgct gagtatctca aaagaattag aattaggtat
    10201 atttccaaaa gtatttgtgc agaagttttc accagaagta ttgggaccgc caccttcttt
    10261 agtctttttt acggatgaat ttgaaactat ttacgagaac atggacgaac ctggagaaat
    10321 cacagacaac aatggcgtct tgactttcaa tttttcggac cctgaaggca attattttgt
    10381 tataggaaaa gctgagtcgt ctgattgaat aaaaaggagg aaaaacagtg gatctaatgg
    10441 gaaggctaga aggttatttg aagtatcggc gagcaaaaca tagtgagaag cttcgagcaa
    10501 ggaatctcaa atcagggaaa aagaagacaa aaggctgggg aaaaatggtc gatacaggtt
    10561 ttggcggact aaataaccta aatcctgtcg atacaaaaga attcatcaaa gaagagcaga
    10621 aacgaaatgg taaagtcaaa tcacactatt gattttgata ctagaatgaa gaaacttttt
    10681 taacacaata aagaaattct tttgatccaa agtgaatgtt catggacttt gagataaagg
    10741 gatttgaaca gatggaacgt tcggattttg cttaaaatcc gaattttttg tttacttatg
    10801 ttttgtttta gtatatgata aaacacgttg ttttattaat caatcgaaat tttaacggac
    10861 tttatttata atgttcggat ttagggggat ggaagatgaa ggaaaaaata ctttcctact
    10921 tcgaaataga aaaattgaat accacggtaa aacgtgaggt actggcagga tttacgacat
    10981 ttatttcaat ggcctatatc ttatttgtaa atccaactgt attaggcgct tctggaatgg
    11041 atgaaggagc ggtgttcact gcgactgcat tagccagtgc aatcggctgt attttgatgg
    11101 gggtattagc ccgttatcca attggaacgg cacctgctct tgggatcaat gctttctttg
    11161 cttattctgt ttgtttaggg atggggatcc catgggaaac agcattgtcc ggcgtattcg
    11221 ttgcttcttt gatctttatt ttgattacaa tcttcaaact gcgggaaatg atcattgatg
    11281 cgatcccagc agatttgaaa tatgcgattt ctggtggtat cggtctattt attgctttcc
    11341 ttggtctgag cgaaggtggg atcatcgttt cgaatgaatc tacattagta ggattaggat
    11401 cactcaacgt tggttctact tggttgacga tttttggatt agtcgtcacg gctatgttgc
    11461 ttgttcgacg tgtaccaggt ggcattttca tcgggatggc agctacaacg attttaggtc
    11521 tggtcacagg tttgattcca atgccttctg aaattgttgc tgcagcacca agtttgaaac
    11581 caacattttt agttgcgtta aaacatgtag gggacatcaa ttctcttcaa atgtgggtcg
    11641 tagtactaac attcttgctt gtgactttct ttgatacagc aggaacttta gttggattag
    11701 caaatcaagc aggctttatg aaagacaaca agatgcctcg tgtagggaaa gctttagctt
    11761 ctgattcaac agctatgctt gcgggctcct tattaggtac ttctcctgtt ggtgcattcg
    11821 tagaatcatc agctgggatt gctgttggag gacgctcagg aatcacagct attactacag
    11881 ggattttctt cttgttaggt ttgttctttt ctccattact gtcagttgtt acttcacaag
    11941 taactgcacc agcattgatc atggtaggtg ttttgatggc tcaatcatta cgtcaaatcg
    12001 aatggggaaa attagaaatt gcgatccctt ccttcttgat cttgattggc atgcctttaa
    12061 cttatagtat ttcagatggg attgcattag gttttatttt ctatccaatt acgatgttag
    12121 cggctaaaag aggaaaagaa gtatctccaa tcatgtatgg tctgttcttt gtattcgtgg
    12181 gattcatgtg gatattgaat gtgaaataaa gaggttgtga aatcagcgtt ctggcctgag
    12241 tattagagaa aaaaacgaaa aaatagttcc tcctattttg ccgtttttgg agctaatacc
    12301 gagggacagc tgagtgaaca ccgtttataa agaggttgtg aaatcagcgt tcttccctgc
    12361 atcaatcaat cctttcaaaa aacagttgag gaaatttcct cagctgtttt tttgttttat
    12421 tctgttaccg ataacaaggt gcaatgacta tttgaaagcg ttacaatttt tggtagaaga
    12481 aactaaacta gatgattaag cgaaaaatag aaacatccat ttataggagg aaaatcatgg
    12541 aacgaaaatg gtggcaccat tcagttgttt atcaaatcta tccaagaagt tttcaagatt
    12601 caaatcaaga tgggattgga gacttgcgag ggattatttc cagattggat tatctggcat
    12661 atttgggcat tgatgtgatt tggctttcgc cggtgtacga gtctccaaat gatgacaatg
    12721 gctatgatat cagcgattat caagcaatca tggcagaatt cggtacgatg gaagaaatgg
    12781 aggaactgat tgaagaagca aagaaaagaa acatccgtat cgttatggat ctggtggtga
    12841 atcatacttc tgatgaacac ccgtggttca ttgaggcacg taaaaataaa gaaaatcctt
    12901 atcgtgacta ctatatttgg cgtgatccag tcaatggaga ggagcctaat ggattgcgct
    12961 cgatattcag tggacctgct tggcagttgg atgagcaaac agggcaatat tttctgcact
    13021 tgtatagcaa gcgtcaacct gatttgaatt ggaaaaacaa acaggttcgc caagcgattt
    13081 atcaaatgat gaatttctgg atcgacaaag gtattggtgg ctttcgtatg gacgtgatcg
    13141 atatgattgg caaagaaccg gataaagaaa tcacaagtaa tggacctaaa ctgcatgagt
    13201 atcttcagga aatgaatcaa gcaacttttg gcgacaaaaa tctgatgacc gttggcgaaa
    13261 catggggagc aactaccgag attgctaaac tttattccga tcctaaaaga aaggaattat
    13321 ccatgatctt ccaattcgaa catataggat tagatcagca agaaggcaaa gacaaatggg
    13381 atttgaaacc tttaagtatc tctgaactga aaaaagtatt atcgaaatgg caaacttctt
    13441 tgggtgatga aggatggaat agtttatttt ggaataatca tgatctgcct cgaatcgtct
    13501 cacgttgggg aaatgacgat ttgtatcgaa aagaaagtgc caagatgttt gctatcttgc
    13561 ttcatatgat gaaaggcact ccttatattt atcaaggaga agaattaggc atgacgaatt
    13621 gcccaattga tgatatctcg gaagcaaaag atattgagac aatcaatatg tataacgaaa
    13681 ggatttcttc tggatttaca aaagaggaaa tcttggaatc aatcaatgcg aaaggaagag
    13741 acaatgctag gacgcctatg caatggaacg cccgtgaaca tgctgggttt actactggaa
    13801 ttccatggct tcgagtcaac ccaaattata aagaaatcaa tgcagaagaa gcgttagcag
    13861 atcaagattc tgttttttat atgtacaaga aattgatcga tttgcgtaga aaacatgaca
    13921 tcatcgtctg gggagactat gaattagtag aagagacgcc agagcatgtc tttgcctatt
    13981 ctcgcatata tgaagaccag cgatggctcg ttgtttgcaa tatgtccagt gaaaaacagg
    14041 tgttcactgt tccacgtgaa gctttaagga ttattgtgtc aaattatcct ctgaaacagt
    14101 taccagacgg caaagtcgag ttacgaccat atgaagcatt tgtgatggaa gtaaataaag
    14161 aaaagcttat ttgattgaaa cgtattaccg agaggttgtg acacattttt tgtcacaact
    14221 tctttttctc aaaactaaac agaggaggcg gtcattactc cgtgtttttt gacaaaaaat
    14281 aaaaatcata ctatgatatg tacgccaatg gaggggatga aatggaaaag ccaattgaaa
    14341 aagcgttgga tgaatgtctt ttctttagcg tgaaaaagct agatcgccta ttgaataaat
    14401 tagcggatga agcgtttaga aaaacaggtc tggcaccaac ttatggattt attttattga
    14461 tattaaaaga aaaagatggt ataccgcaaa aagatattgc acaaatgctc tattctgcgc
    14521 cttcaacaat cgctcgtttt gttgaaaaat tagaatataa aggatacgtc aaaacagtta
    14581 gcgaagggcg cttgtcatta gtttatctta cagaacaagg acgccaattt actaaggaag
    14641 tcgatggtag ctgggaagaa ctccatcagt cgtacaaagc agtcttggga aatgaagcaa
    14701 gtaaagaatt agccgagata atgaatgaag caacagataa gttacaaaaa aaataaaaat
    14761 ttttttaagc aaattagttg catgtgcaac taaccgagtg gtttaatgat aaatgattct
    14821 cgataggtta atcagagcga attatgggag gaatgacaat gtcacaattt gatcaatcaa
    14881 ttattttacc ttcaggagtt caattgaaga accgattgat gctgtcgcct atgacgacac
    14941 aactcagctt cttcaatgga gtgatcaccg aagatgagat cacttattat aaacaacgtt
    15001 ctaaaggact tggtgctgtt attactggtg ctgcaaatgt ccaagatatc ggaaaaggct
    15061 ggccaggcga actgagcatc gctcacgatg aaatgattcc tcggttaagt gaactagcta
    15121 aagcaatcca aggagaaggt gcaaaagcga ttgttcagat tttccacggt ggacgaatga
    15181 ctagtcgagc tactctatcc ggagaacagc ctgtatctgc aagtgcagtt gcagcggaac
    15241 gacctgatgc agaaacaccg cgagcattaa gtgaaaatga aatcaaagaa acaatccaag
    15301 catttggcga agccacacgc agagcgattg aatcaggatt tgatggtatc gagttgcatg
    15361 gagcaaatac gtatttgatc caacaatttt tctctccgca ttcgaataga cgagaggatc
    15421 aatggggcgg tagcttagaa aatcgctatc gttttattga cgaattatta acaacggttt
    15481 tctcaacggt tgaaaaatat gcaaaaaaac cattcatcgt agggtatcgt ttctcgccag
    15541 aagaatatga aacaccaggt atccgttttg aagacacgat ctggcttctt gaacgattac
    15601 gtgagacaaa attagattat ctgcatgttt cattaaatgc gtatgatcga agagcccgtt
    15661 ctgataaata cagcgatcag tcgattcttg aagctgtcca tgacacgatc caaggaaaga
    15721 tccctttaat tggtgtcgga ggcatccgta ctcgcaaaga cgtagagcaa gtactgaaat
    15781 atgcagaact tgcagcaatt ggacagcaaa tgatcgtcga tcctgactgg gcagagaaat
    15841 tattggaaaa ccgtgaggat gaatttgtaa cgaaaccttt tgaagaagca tataaagagc
    15901 tttatttgcc aagtccatta tataatttct tgaaagcacg ttatcaataa ctcaattttt
    15961 atagaaagag gatggaaaaa tgaaagtatt gatcgtatta acaaatgtag aaaagtacga
    16021 aaagatcgag cgtcctacag gtttgtggtt aagtgaactg actcattttt atgatgtatt
    16081 agtaaaaaat gggatcgatg cagattttgt cagtcctaaa ggcggatatg tacctttaga
    16141 accgcaaagt gttttgaata tggatgaaat cgattgggcc tattataaag atgaagattt
    16201 cagaaatcgg gccttgggaa ataccttacg tcctgatgat gtggatgcaa aagattatgc
    16261 agcagtttat tatgctggtg gccacggaac aatgtgggat tttccagaag caacagatct
    16321 tgggaaagtt gcttcacaaa tctatcgaaa tgaaggactg gtctcagctg tttgtcacgg
    16381 tgttgtcgga ttgttgccag ttacagatga atctggaaag tctattttag ctggacgcac
    16441 agtgactgga ttcagtaatg aagaagagga agccaatggt acaacgaatg aagtaccatt
    16501 cttagcagaa gatgctttga aagaaaaagg agctgactat cagtcaggtc cagctttttc
    16561 agaagtcgtt caaatcgatg gacgtcttct tacagggcaa aaccctcaat ctgctcatca
    16621 attgggcgaa gtagtggttg aagcattaaa gcattaaaaa ttgtcagcat aaaaatgttt
    16681 tgcctattct aactattaca ttacacacgc taaaacacgt cttttcgttc ttttctgttt
    16741 cctatttgga cagaggtacg caaaaggcgg gttttttgcc tttctaagag tacatttttt
    16801 gaaacatctc ttcagaaatg atactcttca attaggtcaa ctaatttatt actgagaagc
    16861 agacctagtc acacggtagg atggacagag tgttttctgc tacgctgcta tggatcatag
    16921 gaatgagtaa acatggttta agaacattgt gatttcagcc catgttggca gtcgcacact
    16981 taataagaaa gaggaataca gatgaacatt gtaattgtcg gcgggtcgtt tgcagggatt
    17041 catgcggcta tttatcttcg ccaattgata ccaacaagtg aaatatattt gattgaaaag
    17101 caatcgaagt tagggtggat tcccagcggg ttcaatctga tagtaaaagg aaaggtcacc
    17161 agtgaaaaac aactttcttg gatcaccaaa gaagagctga tcgaccgcta tcgtatccat
    17221 gtttacacag aaaagacggt aataggattg gaagaaaaga aagtcgtttt agcaggcgga
    17281 gacaaactgg attttgatcg tttgattttg gcgactggtt caaatcaaaa ttttcgaaat
    17341 gttcctgcag aagcaaggtt cattcaccca gttaaaaaga tacaaaacat agctgctctt
    17401 cagcaaaaga tcatggatgc aaaaaaaatt gccatcattg gagcaggtca agcagggatc
    17461 gaaatagctg aaggcttagc atcaggaaaa acacaaatcc atctttatga aagccgaaag
    17521 tcgattcttt ttcgttacct agaccctgaa atgacggagc cgttagtaaa ggaaatgaaa
    17581 aatcaaggga tatcgctttt cttagaagag caagtcgtat ctttgtccga aaatgaaacc
    17641 gctgtagtag agacggaaag aagaagggaa aactatgatc ttgttttgct ggctaaccat
    17701 tctcgcccca acaatcagat gtgggaagaa caattaacat tgaatgatga tggaacgatc
    17761 tgggtagatg actatttgca aacgtctcaa aaagacgtct atgcaattgg cgatgcaatc
    17821 caagtcactt ttcgcccaac aaatgaaaaa atgtacgttt ctctagtaaa caacgctgtc
    17881 cgtacagcgc gaaatgtcag caagacaatt agtggttctg cgacaaagga tcagggaacc
    17941 tatcgaccag tcggcaatca gtggtttggc tatttcttag gaagcgtagg tatgacagaa
    18001 gaagaaagca ttttttatcc ccaaaaaatt gaaacagggt acgcggaata caagcttgca
    18061 gcaataaacc agagcaaagt caaagtcaaa ttcctatttg attctgaaaa aaagattctt
    18121 ggagcacagc tccaaagcag aaaagaaatg ttccatcttc ttgaccaact gacaatcgca
    18181 gtagaagaag gctggacgtt cgaacagtta gaagaacatg agctgttttt ccagccagaa
    18241 tatcgtgtac ctaatatggt gtggtcaaag gtggcgaata ctgcctatga agattgaagc
    18301 attattagaa agaaaagagc agttacagat cctgattttg cgaaaccttg ttctgcaagg
    18361 gggaacagcc tctatcaatg gattgagaga gcatgtccaa ctgtcaaaaa cctcttttga
    18421 tcaatatttg gaagatattg aattgattgg acggatgatg ggaaaaaaag tagaagtaca
    18481 gcgaaacgag tatcaggcgc tgcttgtgtt ggacgaagat gtcagtttgg aaaaaattct
    18541 tttgttttta ttacaggaaa gcttgaagtt taaaatgctt gtctatttat tagaacacca
    18601 gcaagtatcc atcgttcgat tagcgacagc gtttaatatc agtgagtctt ctgtttttcg
    18661 aaagatcaaa gaattgaacc agctcttaga ggaatttgga ttacaaatca aaaacggaca
    18721 attatacgga gaagagctac agatccgata tttttattat gaattattcc aatatatccc
    18781 ggaagatcaa cgaccgcttt ttttgcaaaa tacaccagaa aaaagaccat tcattctagg
    18841 attgggccgc gtattagaaa cgacgtttac ggcatcagca gaagcacaga ttgcttgctg
    18901 gctcgggatc acgaaaaaaa gactattgaa tgaaaaaagt acgtacgaaa ccttgaaaga
    18961 aaagaaatta ctctatcagt cggaccggct ttatcaagca atcgatccaa tcatcgtgat
    19021 gcatcttagt cgaacagcag cagaactgaa tgtatacgaa acaatgatgt tttacagctt
    19081 ttttgtttct ttttcgatag tcgacgaaga aatattttat caatatgatt tgacacggag
    19141 caaaaagtta cccaccgctg ttttggatac ttatattcgg gaaacgatgc tatggcatta
    19201 tcgtcctcgt cgtttgaaaa tcaaagagga aaaagcagta ggctaccaaa tttcacaaat
    19261 caacaatgaa tggtttttct ttgttggaaa gatcgaagtc tacgagcgtg atcggctttt
    19321 ggagcagcag caaaaaatgc ttgggcattc cttaacacag ttattggaga aactacagga
    19381 aacagcgatg cagcaattgc caaggaaaag caccgaagac agtgcactta gttatctgat
    19441 gatccagtat gccaatgttc ttttgatgat cgatttttat attgcgaaac cagtagtgat
    19501 agggatcgat ttagaaagtt tgcctattta ccgtattgct ttccaacagt atctgaacag
    19561 agaactgaga ggagtcagcg gtattgagat tgaaagttat gaagaaggaa aaaactatga
    19621 tttggtcata acgttttgtc agagaaacaa gcaacagaat gaatattatc tatcagaatt
    19681 tgcgtcgcct tatgacatta tccgattgaa aagaagaata gaaatgttga aaaaagaaaa
    19741 gaattgatca aaaactagca tcataataaa aaactgtcaa aaaaaagaaa aaaagaataa
    19801 aattttgaca gtttcttttt ttaaatgagt agtatgataa aagcagttaa agaaagcgtt
    19861 tgcagataat ggagggaatc acaatgacag aaccaacgta tatcatggcg attgaccaag
    19921 gtacaactag ctcacgagca attatctttg acaagaaagg aagacatatc ggcagttctc
    19981 aaaaagagtt cacgcaatat ttcccgcaag aaagctgggt tgaacatgat gcaaatgaaa
    20041 tctggaattc tgtacagtca gttattgccg gagctttcat cgaatcaggg atcaaaccaa
    20101 accagattgc tgggattggc ataacgaacc agcgtgaaac aacagtaatc tgggaaaaag
    20161 atacaggacg tccaatctat catgcagttg tttggcaatc cagacaatca gctggaattg
    20221 cagataactt aaaaaatgaa ggatatcaag aatttttcca cgaaaaaaca ggactagtga
    20281 tcgatgccta tttctcagca acgaagattc gctggattct tgatcatgta gaaggtgcgc
    20341 aagaacgcgc tgaaaaaggc gagttaatgt ttggaacgat tgattcgtgg ttagtatgga
    20401 aactgaccga tggacaagca catgttactg attactcgaa tgccagtcgg acgatgttgt
    20461 tcaatatcca tcagctagat tgggatcagg atattttgga tttattgaat attcctcgag
    20521 tgatgttacc aaaagttact tctaactcag aagtttatgg ttatacgcaa ggttatcatt
    20581 tttatggaag cgaagcacca atttcaggga tggcaggaga ccagcaagct gcattattcg
    20641 gacaaatggc attcgaacca ggtatggtca agaatactta tggtacaggt tcatttattg
    20701 tcatgaatac cggtgaaaaa ccgcaattat ccaaaaataa tttattgacg acaattggtt
    20761 acggtataaa tggaaaggtt tactatgctt tagaaggaag tatttttgtg gctggttctt
    20821 cgattcaatg gctgagagat ggtcttcaaa tgttgcaaaa ggctagtgat tcggaagccg
    20881 cggcaaaacg ctccacaaac gaagatgagg tctatgttgt tcctgcattt gttggactgg
    20941 gtgctcctta ttgggatcag gcagcacgag gggctatgtt tggtttgaca cgtggaacga
    21001 caaaagaaga tatcattaaa gctactttgc agtcgattgc ctatcaagtg agagatatca
    21061 tcgatacgat gcaagatgac actggaatca agattcctgt attgaaagtg gatggcggtg
    21121 cagcaaataa tgaatatctg atgcaattcc aaacagatat cttgaatgta ccgatccaac
    21181 gagcagaaaa cttggaaaca acagcactag gggcagcatt tttagcagga ttagctgtcg
    21241 gctattggaa agatacagac gagattcgtg aattttatga agctggcaag ctatttgaag
    21301 ttcaaatgga ggaagagcgc cgagaaaaac tttacaatgg ctggaaaaaa gcagtaaaag
    21361 ctacccaagc atttgaataa ggaggaaatc catatgtttt caaacaagac aagacaagat
    21421 agcattcaaa aaatgcagca agaagaattg gatctgttga tcatcggtgg cggaatcact
    21481 ggtgccggtg tggcagtcca ggcagcagca tcaggaatca aaacaggatt gatcgaaatg
    21541 caagattttg cagaagggac gtcctctcgc tcgaccaaac ttgtgcatgg cggtattcgt
    21601 tatctgaaaa catttgatgt ggaagtagta gctgacacag ttggtgaacg tgcagtcgta
    21661 caaggtattg ccccacacat cccaaaacca gatccaatgc ttttaccaat ctatgaagat
    21721 gaaggagcaa caaccttcaa tatgttctct gtcaaagtag caatggacct ttacgacaaa
    21781 ctggcaaatg tgacaggaac taaatatgag aactataccc tgacaccaga agaagtattg
    21841 gaaagagaac catttttgaa aaaagaaggg ctaaaaggtg caggtgttta tctggatttc
    21901 cgcaacaatg atgcccgttt agtgatcgat aatatcaaaa aggctgcaga agatggggct
    21961 tatctagtaa gtaaaatgaa agcggttggc tttttatatg agggcgatca aatcgttggt
    22021 gtcaaagctc gtgatctgct gacagatgaa gtgatagaga tcaaagcaaa attagtgatc
    22081 aatacgagtg gtccttgggt agataaagta cggaacttga attttacgcg tccagtctct
    22141 cctaaaatgc gtccaaccaa aggaatccat ttagtcgtag atgcgaaaaa actgcctgta
    22201 ccgcaaccca catacttcga tacaggaaaa caagatgggc ggatggtttt tgctatccca
    22261 agagaaaaca aaacttactt tggtacgaca gatacggatt accaaggaga ctttacggat
    22321 ccaaaagtca cacaagaaga cgtggattat ctattggatg taatcaacca tcgctatcca
    22381 gaagcaaata tcacattggc agatatcgaa gcaagctggg cagggcttcg tccactattg
    22441 attggtaatt ctggttctga ttataatggc ggagataacg gatcgatttc agacaagagc
    22501 ttcaataaag tggttgatac agtaagtgaa tataaggaaa ataaagtttc tcgtgctgaa
    22561 gtagaagatg tgttgaacca tttggaaaac agccgcgacg aaaaagcacc ttctacgatt
    22621 tccagaggta gttctttaga aagagaacca gatggcttgt tgactttatc aggtgggaaa
    22681 atcactgatt accgtaagat ggcagaagga gctttacgat tgattcgtca gctgttaaaa
    22741 gaagaatacg gaatagagac gaaagaaatc gactctaaaa aatatcagat ttcaggtgga
    22801 aacttcgatc caacgaaatt agaagaaaca gtgagggaat tagcaaaaga aggagtagca
    22861 gccggtttag aggaagaaga tgctacttat atcgctgatt tttacgggac taatgctcga
    22921 cgtatctttg aattagcaaa agaaatgaca ccttatcctg gtttgagtct cgctgagtca
    22981 gctcggttac gttatggctt agaagaagaa atggttttag ccccaggtga ttatctcatt
    23041 cgtcggacga atcatctgtt gtttgaacga gaccagctgg atgagatcaa gcaacctgtg
    23101 atcgatgcaa ttgctgagta ttttgggtgg acagaagaag agaaggtgca acagactaaa
    23161 cgtttagaag cattgatcgc agaatcagat ctgcgggaac taaaggggga gaaataaatg
    23221 aacagtgata tgactcaaat catgggggag tttattggga cattgatcct agttttgctt
    23281 ggagatggtg tgtgtgcggc tgtgaatcta aacaaaagta aagcacaagc ttctggctgg
    23341 attgtcattg cgtttggttg gggactcgct gtaacaatgg ctgtctatat ttctggattc
    23401 atgggcccag ctcatttgaa tcctgcagtc tctctagcta tggcgatgac aggggcaatt
    23461 agttggaatc ttgtcgttcc tttcatcatt gcgcaagttt taggagcttt tgcaggggcc
    23521 attcttgttt ggctttctta tttgccacat tggaatgcaa cgaaagatga aagtgcgatt
    23581 ttgggtacgt ttgcaacagg tcctgctata cggaactatc cagcaaatgt aatcacagaa
    23641 ttaattggta cttttgtatt agttcttggg ttgctagctt ttggacaaaa cgaatttgca
    23701 cctggaacga acgtattcgc tgtaggtgga ttgatcttag ccatcggtct ttcattagga
    23761 ggaccaacag gatacgcaat caaccctgct cgggactttg gtcctcgatt agcacatgcg
    23821 gtattgccaa tagctaataa aggaacttca gattgggcat attcatgggt accaatcgca
    23881 ggtccaatga tcggagcaat tatcgcggta ggtgtctatt cattaatggt gtaaatctat
    23941 aaaacatact aaggtttgac aatcaaaagg cgtcttgcta tagttaagtg gaaaataaat
    24001 aagacctaga agaatgggga atgttttgtg agtgtagctg gtatctaatc agtaagtcga
    24061 atagccaata aacaaggttt atttggtgtg cttatgaaac ggtacctgtc aggaaaacaa
    24121 tatctttgta ttctaataag gataggacat gactttttta agctgtgttc tatcctttta
    24181 tttatctcta ggcccttatt tattttggga caggtatctc ttcaagaaaa ggagagagta
    24241 tgaacaacga aatgattagt caattacaat ttgatcagat caaaaaggaa atccaagcaa
    24301 gagcaatcgg aaactatagc aaaaaacgaa tcagtaagat gacgacctcg tcaaacctac
    24361 aaaccgtctt ggatcgtcaa gaagaaacaa gagaagcacg cttgatctta gaaagtagtc
    24421 agcatgttcc atttatggga ttgccgagga ttgatgcact gacagaacaa gtcaaaaaag
    24481 gattggttct gtcgcccgct gatttgatcg aatatgcgga ttttctaaga agcagtcgga
    24541 tgatcacaaa gttttttgat aagaatcaat accaggcacc tttgttattt gcttatagca
    24601 agcatcttcc tgatttgata aatgtagaag agttgattga ccaaaaaata aaaaataata
    24661 aagtcagtga cgatgcttca cgtaatttgc gtaaagtacg caaacaatta caaataatag
    24721 aaaaagaaat ccagtcaaaa ttgctgaaat tcttgcgtca tccgaaaaac aaagaaatga
    24781 tccaagaagc aataatcgtt caaaaaggtg aatgctatac gattcctatc aaagccagct
    24841 ataaaaataa agtagatggg acgatcatcg atgaatcgaa caaaggaacg acggttttta
    24901 ttgaaccgac tgtggtttct aaactgaatg aacactatca gttgttaaaa gctgaagaaa
    24961 tcagtgaaga atatcaagtt ctggctgcat tgactggtgc tattgcagaa aatgaagaag
    25021 caatcgattt attgatcgaa acgatgacgg tattagacat catttttgct cgggcaaaat
    25081 tcagtcgaga aatcaatggg atcacgccta aaatcaataa atcagaacat atcgtcatca
    25141 aacaaggcag acatccattt ttacctgatc atgcggtacc attggatgtt gaaatcggta
    25201 aagactatcg aggcttgatt attaccggag caaatgccgg aggcaaaaca gtcgtgttga
    25261 agacagtcgg tttattgact ttgatggcga tgttcggtat gcaagttccg gcaaaagaag
    25321 ggacagagct tgctgtcttt gatgagattt tcgtcgatgt cggtgaccac caaaatttag
    25381 aaaatgcgtt gagtacattt tctggtcata tgcaaaacat cgctgctatt ttgaaaaaga
    25441 taaaaaggaa tacgttggta cttttagatg agattggcag tggaacagag ccaaatgaag
    25501 gggctgcact agcgatcgca atcatggaat ctatgtatga gcaaggggca ttgatcattg
    25561 ccacgaccca ttatggagaa atcaagaaat tcgccggaga tcatgaagat tttgtgccgg
    25621 ctgctatggc tttcgatcgg gaagcacttc gtcctaaata tcagttgcgg gttggagaaa
    25681 caggagaaag ccaagctttg tggattgctc ataaaatgga tatgtcgatg aaattgatcc
    25741 atcaagcaga gcgttacttg gcggaaaaag cctatcggac acagaaaaaa gaatttcttc
    25801 ctaagacagc agttacaaat aggaaggaga ataaaaaaga acgccagctc tttgcaaaag
    25861 gtgatcggat atttgtcaat gaataccaaa aagaagcgtt agtttatgaa gatataggtg
    25921 aagatacgat tgatgtatat ttagacaaaa aaataatcca cgtgcctcgt cagcgtgttc
    25981 gtctggtgcg ttcagcagaa gatctgtacc cgacaggtta cgatttagac agtctgttta
    26041 tcgattataa aacacgtaaa cggcagcgtg acctagagcg aggatcaaaa aaagctcata
    26101 aagtactagt aaaagaaatg cgcaaacgac aggaagaaag aagagtaaac gatgaaaaca
    26161 gcaagtgatt atctcaaatt agctcataat atcagtggct gtaaagaaaa ataaaaacag
    26221 tgaaatcatt ggttttattc cccgtgtaag ctaaaataaa aagaaaagag tgctgaggag
    26281 gtagggcgat aaaatgaaat taagcacaga ctggcgtaaa gaaatacaaa cgattccgaa
    26341 tcttttatcg atattccgta tttttttgct gccgatttat ctttattttg tgctgagaca
    26401 gtcattttat atagcaggag cggtcattgt tgtctcaggt ttgtctgatt atctagacgg
    26461 ggtcattgct cggagatata atcaggtaac agacttaggg aaggtgcttg atccgtttgc
    26521 agataaattg acacaacttt ttctgatttt gtctatggca tggtatcgac cgtggctttg
    26581 gctgttgttt ggtctattct tgataaaaga aggctttatg tttgtagctg gattaatcgg
    26641 gctatctaaa aatatcaaat taagtggagc gaagtggtat ggcaaagtag cgactgctgt
    26701 catctatgtt ggcatgatcc ttttgctgct tttcccagaa ttaccaacgc tgtgggtgag
    26761 agtcattttt gccgtcatca cttacggtct tttacaatct tttgtcttgt atgctgtcga
    26821 gtaccgaaag atgtttcaaa gaaaataaaa aaggggtgtg acctatgtcg cattcctttt
    26881 tttgataaat agactcccag tagctacttc tcgataataa gccaaccaaa ccaaaaacag
    26941 gagaagttgt ttttgaaatt atttcttgtg attcggagtt aaatggtagg ctcacaacct
    27001 cttggtaaac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat
    27061 gagcagctat ttttccgttt gtttctctaa tactcaggtc aaaccgctga ttttacaacc
    27121 tcttccttaa acggtattca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
    27181 tgtagaaatc cattttcaca aaatttttct taatactcga aactaagcga ctttttcaca
    27241 atctcttcct taaacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa
    27301 aatatgagga gctattttcc cgtttttttc tctaatactt aggtcaaacc gctgatttca
    27361 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
    27421 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcgtt
    27481 ttcacaacct ttgattcacg gttttccaga agtagcttct tcgaaaaata agccgaaatt
    27541 tcttgaagct gatcacttct gtcgcaacct ctactgacta aggcttgata taagcataac
    27601 cttgggattg cagttcaact aaatcaagga cacctgctgg tacaacgatt acttgttcag
    27661 gtaaagaact ttctgaaata tgattagcac gcatagcatt ggcgcaagcg tgaaaaacga
    27721 ctccttgcaa atctaagaat tctgcattgg cagagtctag ataaccggta atcgcgatgc
    27781 catttacaga tacgacaatc gtcgtttctg gagaagcttt tatcaggttt tttacgtttt
    27841 tccctgtttc tgaccatttt tctaattcat caatatggaa aacgactttc attgaaatca
    27901 ttcctttact ttttaataat catcttgatc gcttgttcta gtttagccgc ttgttcttct
    27961 ggattactat caggtttctc aaagcaattt ttcagattat ctgcaacgat catgcccatt
    28021 acgcgatcaa tactagaacg aacagcgctt aattgagtga tgacatccat acattctttt
    28081 tcgtcttcga tcattttttg gataccacga atttgtccct ctgtacgttt caaacggttc
    28141 aggatatttt tattatcaat ctccataaca cattctcctc tatttttata attagataat
    28201 accccttatc ggtattttat gcaaatataa aaagtagtga aaaaaatgaa gagtcattct
    28261 ggttgacata ttaataaagc tcctgtttaa tataccccat gaggtattta taaaaatgta
    28321 aatgaggtgc agaaaatgaa tttgctattt caatcaataa aatcgataac agtcccagaa
    28381 ttaaaggaaa agcttttgga aaatccagcg ttacttgatg ttagaacacc tgcagaatac
    28441 cggggcgggc atataaaagg agctaaaaat gttccattac aaagtataaa cagatatgat
    28501 ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag ggatgaggag taaacaagca
    28561 gctaaagaat taaagaaatc tggttatgat gtagtgaatg tacgtggtgg catgaatcaa
    28621 tggtttgatc gaacagtagg agggaaataa gatgaaaatc gttattgtcg gaggtgtagc
    28681 aggtggaatg tctgctgcta cacggcttcg ccgattaatg gaagatgcag aaatcgttgt
    28741 ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc ttgccttatt atctttcggg
    28801 agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca gaatcattat ccgctcgttt
    28861 ttgtttagat gtgcgtccaa atcatgaagt gacagccatc tttcccgaaa acaaaacggt
    28921 agaagtcgta catgagggtc aaaaacacat tgaacagtac gatgcattgg ttttatctcc
    28981 tggtgcaaaa ccagttgttc catcgattcc agggataaca gaagccgaca atgttttttc
    29041 tattagaaat gtaccagata tcgataaagt gatgcatgca ttagaaaaac agccaaagcg
    29101 tgccgtgatc gttggtgcag gattcatcgg attggaaatg gcagaaaacc taaaaagaag
    29161 aggtttagaa gtcatggtga tcgaacaagc accacatatt cttccaacac tggatgaaga
    29221 aatggcagct tttatagaaa aagaattgtc tcatcaagga gtagaagtga ttacttctca
    29281 tgctgtcgct ggatttgaag accacgggaa acgattgcga ctggatgatg ggcgtaccat
    29341 ccctgctgat ttagttattt tatccattgg tgttcgtcct gataaccagc tagcagtgac
    29401 cgctggaatc gaattaggca tacgcggggg tatcctagta gacgaacgtt atcaaacgaa
    29461 tattcctgat atttatgcgg tgggggatgc tatcgttgta aaacagcaaa tcactggaaa
    29521 agatgcactt atttctcttg cttcaccagc caatcgtcaa ggtagacaag ttgcggacac
    29581 gatttccgga atttctcgaa gaaatcaagg cggtattgga acagcaatta tacgaacgtt
    29641 tggaatgact gccgcatcca ccggtttaag tgaaagaaca gccaaagaaa acgaactgtc
    29701 ttttgaagtc attcatgtat caggaaaaga tcatgcaagc tattatccag aagcaacaga
    29761 tattttactg aagttgatct tccatccaga gactggcgag atttatggtg cacaaggtgt
    29821 tggggcaaaa ggtgtggata aacggatcga tattttagca acagcaatca aagggcattt
    29881 gacgatcttc gatttaccgg aattagaatt gacgtatgca ccgccatttg gctcagccaa
    29941 agatccagta aacatgctag gatatgcagc aatgaacatt gcagaagggc ttagtgaaac
    30001 cgtacaatgg catgaattgc cgacagaact agcaaaagga aaaattttat tagatgtgcg
    30061 aacagcagaa gaattggaaa agggcaaatt caaggaagcc aaacatatcc ctttgaatga
    30121 acttcgggac cgattaaatg aattagacag ccaacaagaa tatatcgtca gctgtcatag
    30181 cggattacgt agctatctag cggaaagaat cttgaagcag tctggctacc acgtaaaaaa
    30241 ccttgatggt gcattttctt tatatcaaac tgtccgacct gaagaactga tatatcctaa
    30301 caaatgatac tcgttaaaaa gcttgcattc gactcttaaa agaggtaaac taatcggttc
    30361 caccacttta aaaaaagagg agaatgtgta tggtcaacac acaaaaacag ttagaagaag
    30421 catatctgaa gaacgtgtat caaaagctaa ttgataaaaa aaacgaactg cgggaatcga
    30481 tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga aatggctggc gagcttcgat
    30541 tgaattttga cagttactta gataacctgg atactttttc gatgattgaa atgaaaaata
    30601 gagaaatcga tcagatgaat atcaaacaga aaaacgcaga aaatcagcta gcaaagatcg
    30661 aacgtttatt gaaaagtccc tactttggaa aagtcgttgt agactttctg gataatgagc
    30721 caaaagaatc cttttatatc ggtacagcaa atttcacaga tgatgcagat gaaaatcttg
    30781 tttatgattg gcgttcacca attgccgaac ttttttacaa taacatgatt ggtccatctt
    30841 cttatacggt tcgtcaaaat aaaatcgata cggttattaa agaaagacga caatttcttt
    30901 tggaaagaga tcgtttggtc aagttttttg atacgtctgt agctattcaa gatgacgtat
    30961 tattagaagt cttaggccaa gatgagacca atgaaatgaa ggctatcact gctacgatcc
    31021 aatcggaaca aaacacaatc attcgggatg tgaaaagtca aaatatcttg gtaaatggcg
    31081 tagcaggaag tggtaaaacc tctgctatca tgcagcgtat tgcgtatctg ctctatttat
    31141 atcgagatac gatgacatcc gatgacttgc tgattctatc acctaatcat cggtttatcg
    31201 actatatttc gaatgtccta ccatcattag gtgaaagaaa tccactgaat ctaaccatca
    31261 tacagctggt ttcacagctt tcagcagagg aaatagaagg tgaagaggct tatttcaaac
    31321 gtatcacggg agaaaatgta tcggagcaga cagaacgttt gcgtagcaaa aagtttatcg
    31381 ataatctaaa acaatcagat cctttgtttt tagatcatcc gaattttatc cgtggactta
    31441 ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga aaaaatatat gagaaagttc
    31501 ctgctcatcc taaattgatc gataggcttc aagcaacgaa aaaggcactg atgagcgaat
    31561 ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt gcagaatcag gtactctcgt
    31621 tgacagaaga taggcagttg gaattattcg gtaaattgat ttctgatgat tcggaacaaa
    31681 gtattgtagc ttatgcaagg aaacttttac aaaaaaaata tcgaaaaata accagacaaa
    31741 tcgaagaaat ggcatgggtg gatgagcatc agttgtttga aagaatttat gaaaaacgat
    31801 atggctcagc ttatgcttgg caacccacac gtactgttga tgaagcagtc attatattag
    31861 ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc atttcgctat ctattgattg
    31921 atgaagtaca agattatacc ctggcacaat taggtctatt gatcgaatta tttccaaaaa
    31981 ctcactttac tttagtaggt gacgagaatc aggcaatctt taattcttcc actacttttg
    32041 ctgatattat gcgttgtttc gatgactacc atctgccgat ccatcggtat gatcttagaa
    32101 atagttaccg ttctagcgga gcaatcacag agcttttcaa aacctatgct gtggatcaag
    32161 agaagatcga catcgtatcg atccgaccgc aaggaaaaga acccgaatac cgctcttttc
    32221 gatcctctga agaattgttg aaaatactag ctgagatttt ggtttctcta aaaggagaaa
    32281 aagcggcagt tatcacccaa acagaagagg aaacccaaac gctacaagaa tcgattaaaa
    32341 aaaattcata tggattagaa aactgccaaa tcattccttt gagtctagca aaaggactgg
    32401 aatttgatca tgtaatctta tatccatttg aaaacgatgg agatgagcaa agaagacgca
    32461 gacaaatgta cacggcgata tctagaggaa tgaaaagcat tgtcgtattg gaaagagcaa
    32521 cataattaag agataaaaaa taaaaagaac aactgataaa aagaacaaaa tatttttcat
    32581 ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa tttaggaaca ctttatgtga
    32641 aaagaaatga aatgtatggt aaactcctac gtcgtaacaa aaaaacgtga ggtgaggata
    32701 tgaaaaaagt aacaagagaa gaagttgcat caattttggt gaaagataag tatttttcaa
    32761 aaggtaatta ctggttaaag atttggcaaa cgttagtggc tcttttagga tggatgatcg
    32821 ttgttcttcc ttttatatgg gtctttttcc cattaacgag accagaaaga gccaaggatt
    32881 tttccttgta cgctttttta tcagaaaaga aaatgctgta tttcttaatc ggcttttttg
    32941 tattgatttt tttcagtttc ctgattacct catttgtatt gactaggtgg aacaatcgaa
    33001 atctatacaa aaaagtaaat aaatcttttg aatacgccgt ttgtataatt aagcgagaca
    33061 aataaaaaag ccatttatgt tacactc
//
LOCUS       DAPBBG010000030        31723 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPBBG010000030 DAPBBG010000000
VERSION     DAPBBG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.030"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="RD477_001606"
     CDS             6..305
                     /locus_tag="RD477_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7633966.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="RD477_001607"
     CDS             378..1739
                     /locus_tag="RD477_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDT7633967.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="RD477_001608"
     CDS             1761..2021
                     /locus_tag="RD477_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633968.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="RD477_001609"
     CDS             2035..2787
                     /locus_tag="RD477_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDT7633969.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="RD477_001610"
     CDS             complement(2881..3486)
                     /locus_tag="RD477_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7633970.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="RD477_001611"
     CDS             complement(3539..4513)
                     /locus_tag="RD477_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDT7633971.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="RD477_001612"
     CDS             complement(4891..5610)
                     /locus_tag="RD477_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7633972.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="RD477_001613"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="RD477_001613"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDT7633973.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="RD477_001614"
     CDS             7161..8558
                     /locus_tag="RD477_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7633974.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="RD477_001615"
     CDS             8639..9079
                     /locus_tag="RD477_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7633975.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="RD477_001616"
     CDS             9227..9553
                     /locus_tag="RD477_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7633976.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="RD477_001617"
     CDS             9554..10303
                     /locus_tag="RD477_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633977.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="RD477_001618"
     CDS             complement(10383..10838)
                     /locus_tag="RD477_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDT7633978.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="RD477_001619"
     CDS             complement(11265..11540)
                     /locus_tag="RD477_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633979.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="RD477_001620"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="RD477_001620"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDT7633980.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="RD477_001621"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="RD477_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDT7633981.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="RD477_001622"
     CDS             14770..17436
                     /locus_tag="RD477_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7633982.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="RD477_001623"
     CDS             17800..18552
                     /locus_tag="RD477_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7633983.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="RD477_001624"
     CDS             complement(18574..19728)
                     /locus_tag="RD477_001624"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDT7633984.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="RD477_001625"
     CDS             19913..22354
                     /locus_tag="RD477_001625"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDT7633985.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="RD477_001626"
     CDS             complement(22684..23388)
                     /locus_tag="RD477_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633986.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="RD477_001627"
     CDS             complement(23575..23820)
                     /locus_tag="RD477_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633987.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="RD477_001628"
     CDS             complement(23837..24688)
                     /locus_tag="RD477_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7633988.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="RD477_001629"
     CDS             24854..25858
                     /locus_tag="RD477_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDT7633989.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="RD477_001630"
     CDS             complement(26099..26455)
                     /locus_tag="RD477_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7633990.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="RD477_001631"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="RD477_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDT7633991.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="RD477_001632"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="RD477_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDT7633992.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31576)
                     /locus_tag="RD477_001633"
     CDS             complement(30815..31576)
                     /locus_tag="RD477_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7633993.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
BASE COUNT        10199 a         5650 c         6433 g         9441 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaagatac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatctg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtc
//
LOCUS       DAPBBG010000031        31456 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPBBG010000031 DAPBBG010000000
VERSION     DAPBBG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.031"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(192..377)
                     /locus_tag="RD477_001634"
     CDS             complement(192..377)
                     /locus_tag="RD477_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633994.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1227..1568)
                     /locus_tag="RD477_001635"
     CDS             complement(1227..1568)
                     /locus_tag="RD477_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7633995.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1568..1798)
                     /locus_tag="RD477_001636"
     CDS             complement(1568..1798)
                     /locus_tag="RD477_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7633996.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2094..2432
                     /locus_tag="RD477_001637"
     CDS             2094..2432
                     /locus_tag="RD477_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7633997.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2637..3212)
                     /locus_tag="RD477_001638"
     CDS             complement(2637..3212)
                     /locus_tag="RD477_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDT7633998.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(3577..4158)
                     /locus_tag="RD477_001639"
     CDS             complement(3577..4158)
                     /locus_tag="RD477_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7633999.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDAEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4341..4967)
                     /locus_tag="RD477_001640"
     CDS             complement(4341..4967)
                     /locus_tag="RD477_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7634000.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4989..6317)
                     /locus_tag="RD477_001641"
     CDS             complement(4989..6317)
                     /locus_tag="RD477_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7634001.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERFFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6475..6876)
                     /locus_tag="RD477_001642"
     CDS             complement(6475..6876)
                     /locus_tag="RD477_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDT7634002.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAEMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6962..7168)
                     /locus_tag="RD477_001643"
     CDS             complement(6962..7168)
                     /locus_tag="RD477_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634003.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7263..8765)
                     /locus_tag="RD477_001644"
     CDS             complement(7263..8765)
                     /locus_tag="RD477_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDT7634004.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTNPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTRYAQDLVTKIKKGMGTQDDKPLKGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8783..9238)
                     /gene="argR"
                     /locus_tag="RD477_001645"
     CDS             complement(8783..9238)
                     /gene="argR"
                     /locus_tag="RD477_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDT7634005.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9336..9953)
                     /locus_tag="RD477_001646"
     CDS             complement(9336..9953)
                     /locus_tag="RD477_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDT7634006.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYDEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10174..11547
                     /locus_tag="RD477_001647"
     CDS             10174..11547
                     /locus_tag="RD477_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634007.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEKLQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11622..12734
                     /locus_tag="RD477_001648"
     CDS             11622..12734
                     /locus_tag="RD477_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDT7634008.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTSHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12806..14443)
                     /locus_tag="RD477_001649"
     CDS             complement(12806..14443)
                     /locus_tag="RD477_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDT7634009.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVQGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14611..15219)
                     /locus_tag="RD477_001650"
     CDS             complement(14611..15219)
                     /locus_tag="RD477_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7634010.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPVAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DATYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15604..16755)
                     /gene="queG"
                     /locus_tag="RD477_001651"
     CDS             complement(15604..16755)
                     /gene="queG"
                     /locus_tag="RD477_001651"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDT7634011.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16774..17526)
                     /locus_tag="RD477_001652"
     CDS             complement(16774..17526)
                     /locus_tag="RD477_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634012.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIHFVQLKKEKLYYGLYSR"
     gene            complement(17666..18616)
                     /locus_tag="RD477_001653"
     CDS             complement(17666..18616)
                     /locus_tag="RD477_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDT7634013.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            complement(18617..19456)
                     /locus_tag="RD477_001654"
     CDS             complement(18617..19456)
                     /locus_tag="RD477_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7634014.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19453..20199)
                     /locus_tag="RD477_001655"
     CDS             complement(19453..20199)
                     /locus_tag="RD477_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7634015.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20437..21807
                     /locus_tag="RD477_001656"
     CDS             20437..21807
                     /locus_tag="RD477_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7634016.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21862..22758)
                     /locus_tag="RD477_001657"
     CDS             complement(21862..22758)
                     /locus_tag="RD477_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7634017.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22872..23042)
                     /locus_tag="RD477_001658"
     CDS             complement(22872..23042)
                     /locus_tag="RD477_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634018.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23039..23716)
                     /locus_tag="RD477_001659"
     CDS             complement(23039..23716)
                     /locus_tag="RD477_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDT7634019.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23807..24712)
                     /locus_tag="RD477_001660"
     CDS             complement(23807..24712)
                     /locus_tag="RD477_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7634020.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24887..26341
                     /locus_tag="RD477_001661"
     CDS             24887..26341
                     /locus_tag="RD477_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634021.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            26357..27388
                     /locus_tag="RD477_001662"
     CDS             26357..27388
                     /locus_tag="RD477_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7634022.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27395..28582
                     /locus_tag="RD477_001663"
     CDS             27395..28582
                     /locus_tag="RD477_001663"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDT7634023.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28738..30120
                     /locus_tag="RD477_001664"
     CDS             28738..30120
                     /locus_tag="RD477_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDT7634024.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30092..30478
                     /locus_tag="RD477_001665"
     CDS             30092..30478
                     /locus_tag="RD477_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634025.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30678..31346)
                     /locus_tag="RD477_001666"
     CDS             complement(30678..31346)
                     /locus_tag="RD477_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDT7634026.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8907 a         6360 c         5678 g        10511 t
ORIGIN      
        1 taacagctga aaagatagga cttactctta gtaaagtatc tgaactatta atttttaaac
       61 tatcattttt atcctcaact acatacatat ctgttgcaac ttcactttct gtctcatcca
      121 gataaatgat taaggaatcg ggaattgcaa tatcttcagc tgacgctgga atagttgagc
      181 caaattagtc atcatatctt tattataaaa taaccaacag caaaaaatac aatgaaaaac
      241 aaacttggaa ctaatgtatc attaataaat gttgaaaaat tcagcaactc attactgtat
      301 gattttagca gcccaaccaa catataccct ataaaagcta ataagatggt taggaatcct
      361 ttagaatttt tactcattca taaacacttc cttaaatagc ttttattata gaaggtattg
      421 taaataataa tattgacaaa aagtgactca aaataaaaaa aaccttacat tatactggaa
      481 tgaactgtat tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa
      541 gtaattttat taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta
      601 cttttacatt cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg
      661 cacattttcg agcattacaa gtattggtgt cgttagaaca actaaataga aactagcggt
      721 agatttttca gaatctatgc taatttctgt acggatgttc cagtgatggc aaatttctct
      781 tttcattgtt tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa
      841 attctttcct agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta
      901 aaatgacatt actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt
      961 ctttttcttc tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt
     1021 gaaaaaacaa gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt
     1081 cgtttaaaat gctatatatt ctttagttta agatacggaa attaatccca tcgaccacac
     1141 tagtagaatg aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa
     1201 aatattatag agtttactta caatgcttaa aacatatact gtataatctg atcaatttta
     1261 gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga
     1321 gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa
     1381 cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta
     1441 cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct
     1501 actgagggat caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct
     1561 tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat
     1621 aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg
     1681 ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat
     1741 cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta acaagcatta
     1801 tcatcactcc cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg
     1861 taaactatct agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa
     1921 attgtagcca gttcattttt tctttaaata aaaaaataat ccaagaaaca ttgaacctat
     1981 ggatccaaaa gctacccaaa tactatagct aactccccgc taccttcaat cttgcagata
     2041 aaacgctttg gaaaaaaatc gagaaaatcg caaagacgac aggccatcct atcatgcctg
     2101 attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca
     2161 cgaaactagc aggtgaagaa gtttcaactg ttcggattaa agaatgcccg attcaaatag
     2221 aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg
     2281 tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat
     2341 ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga
     2401 attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa
     2461 aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga
     2521 ccctatagat gcagtacatg cagcttatga gcatttcttc tgttctcttt gtttttggga
     2581 tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat
     2641 accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata
     2701 tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt
     2761 ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc
     2821 tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat
     2881 ttgtcttttt cttcattcca ctcataaaag ccactcattg ggaaaacaca acgattttgc
     2941 aaaaatggtt gatgaaaggt ctttttttct tctactgtct ctgcacgggc attgatcatc
     3001 aattgtcctt ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt
     3061 tcttcctctt ttcttaacgt cacaacaaga tttgacggaa aaacttctcc agctgctaat
     3121 tctgctgcct gctccggttg tttcttctgg gcttctcgat aataattctg aaggagctct
     3181 gactctagat caaaaaaata acgaccacac atcctaatcc ctcccaacct ttttttctat
     3241 catacactgc aacaagaagt tcgtaccaaa aaaacgcttt caatttgtgt agaactaaaa
     3301 aaattgtgac aagtgacaaa ctctccagtg aatggtattc tcctgccaac tccttggtca
     3361 caagtcacaa tattcaacaa acatgagaag ctattttttg aaattgttcc ttgtgacccg
     3421 aagtgaaacg gcaggcttac gacctctggg taaacggtgt tccagaagta gcttcttcga
     3481 aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaatgc cttcttattt
     3541 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
     3601 caatctttct ttgatcaatt ggaaattctg tctaagtagt gatagatctt gtgtgacttt
     3661 caacaataag atactgccct ggatttcagc aatcaacgct ttggcttgac ggtctgcctc
     3721 tgcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
     3781 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
     3841 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
     3901 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
     3961 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
     4021 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
     4081 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
     4141 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
     4201 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
     4261 cagccctcat tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
     4321 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
     4381 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg accaggagca
     4441 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
     4501 gaatcgctac acaagtttcc gttcattcca ccgagaatta ctgtgtcgat tccatttttt
     4561 ctcaattgaa gcgctaagtc gttgctttct ggtccaaaga ttttatgagg agagctgatg
     4621 atcgtatttt catccaaata tggtttcaat tcttcgatga aatcagctcc tgaaccttct
     4681 tcaactgatt catattgact tttcctagca aacatctgct gttcaagcat catgtgttct
     4741 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
     4801 agtttgtatc ctttttcttt tgcttgtttc accatttcga tcatgttttc gatcgtattg
     4861 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
     4921 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
     4981 ttcgttattt attttaatac atcatttaaa gcttcactca ttgttggatg agaatagatt
     5041 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
     5101 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
     5161 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
     5221 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
     5281 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
     5341 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
     5401 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
     5461 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
     5521 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
     5581 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
     5641 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
     5701 aaaatcattt gcgcgatatc ttcatcttca cgaggaagaa attcaggatg ggcatcaaga
     5761 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
     5821 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
     5881 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
     5941 ttgatgaaaa accgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
     6001 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
     6061 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
     6121 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
     6181 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattcg
     6241 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
     6301 ttttgatatt tttccatcct ttttccctcg cttctttttt acctaccgat tggtacaaaa
     6361 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
     6421 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
     6481 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
     6541 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa
     6601 tactacaccg atctgatagc caccatcatc taaattataa agagagacac gtgcttttac
     6661 tgtggactct gctgtcatct ctgcttcttt aatcatgtgc tgtactgcac cattgaaaca
     6721 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
     6781 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
     6841 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
     6901 tttgctactt ggctataact atagtgtatg acaagtaagg cgctttctac aaaaattctg
     6961 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
     7021 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
     7081 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
     7141 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
     7201 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
     7261 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
     7321 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
     7381 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
     7441 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
     7501 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
     7561 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
     7621 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
     7681 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
     7741 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
     7801 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
     7861 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
     7921 atcaaattat ttcgattgat cactgttccg gaagcactga caactgcaga acgatccaca
     7981 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
     8041 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
     8101 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
     8161 ccaccagcac catttccagc atccacgatg attttccaac cttttagtgg tttgtcatct
     8221 tgtgttccca tccctttttt gatttttgtg actaaatctt gcgcataacg tgtcaaaagg
     8281 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
     8341 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
     8401 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
     8461 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
     8521 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
     8581 tgaccaatcc caatcgtcca ttttttattt ggattggttg acttcttctt tttctttatc
     8641 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
     8701 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgtagcgc tttcatatct
     8761 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
     8821 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
     8881 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
     8941 cagtcaatac gatgaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
     9001 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
     9061 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
     9121 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
     9181 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
     9241 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
     9301 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
     9361 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
     9421 tgttctttag aagctctttc atagccttct tttgcgataa atgcttctaa tttctcaaac
     9481 gttgctggtt cttcgtcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
     9541 atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
     9601 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
     9661 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
     9721 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
     9781 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
     9841 ttcgggtcgc cgatccctct gatgctaaag aatggttttt taggaacctc tagtatttgc
     9901 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
     9961 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
    10021 tcgtgttgac actatttttc agtttgtgct agaataagtt aagtaaataa atcgtacaga
    10081 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
    10141 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
    10201 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
    10261 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
    10321 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
    10381 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
    10441 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
    10501 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
    10561 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
    10621 attggtccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
    10681 tttcttgtgc ccgttttgat tctttctttg attatcggct ggtttgctat tcccgagatg
    10741 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
    10801 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
    10861 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccgctg
    10921 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
    10981 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
    11041 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
    11101 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
    11161 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
    11221 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
    11281 aatatgatga ccaccggaat gaatgcactg aatgaaaaac tacaaggaga cgggaacgca
    11341 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
    11401 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
    11461 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
    11521 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg taggtgttgc atctgccgtt
    11581 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
    11641 tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg
    11701 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
    11761 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
    11821 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
    11881 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
    11941 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacaa acatctcaag
    12001 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
    12061 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
    12121 aggcaaaagt cacttcgcat aagaagagcg attcttggct catcaaggga ccgtttggag
    12181 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
    12241 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
    12301 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
    12361 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
    12421 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
    12481 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
    12541 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
    12601 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
    12661 taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc tatattacga
    12721 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
    12781 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
    12841 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
    12901 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
    12961 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
    13021 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
    13081 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
    13141 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
    13201 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
    13261 ttgcaccaaa agacgctgct aaaaccgggt cattgatctt gataattggc ggaagcgaat
    13321 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
    13381 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
    13441 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
    13501 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    13561 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    13621 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    13681 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    13741 caataaaacc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    13801 ctccaaagta acgtaatcct tgcacagccg ctgtattatg gagagcatca aacaaaatga
    13861 gtccattggg aaatgtttca tgtgtccact cgggatcaca aaagatataa agatctccct
    13921 catcaaagga aacagacttt tgataagtct ttaagatctt cgaattcgct ggctgaaaat
    13981 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    14041 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgtttgcgac
    14101 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    14161 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    14221 tcctgacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    14281 tcgttcctgg actccctttt gctagttgat aagctgtctt cctgtcaaaa acttcatgaa
    14341 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    14401 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    14461 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    14521 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    14581 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    14641 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    14701 attggcagct gaatagtcgc cgtttaagta tgtagcatct aattgataac ggccgatgta
    14761 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    14821 agagcttgaa gttggcgctg ttgcagcaac tggggcagca ggagctactt caactgtttc
    14881 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    14941 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    15001 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    15061 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    15121 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    15181 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    15241 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    15301 atcatgacat ttaatgacaa aatgacgaat tatttaatat ttgtaacatt taaacaaata
    15361 tttttttgat acaaaaccat aaaatccttg ttgtataagc aaagtaatcg tttttctatg
    15421 tttttttatt tttgaatgaa aaacgcagat tctgtaacat ttactgcaat aatccataaa
    15481 aaaaaagcaa aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca
    15541 aaaaaaccag taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt
    15601 tttttatttt aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac
    15661 ttcttctgtt tctttagcta atatttcatc taatacatca agccattttt ccggttcttt
    15721 tgttcctaga cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt
    15781 taaacattct gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa
    15841 tgcgtttctc tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg
    15901 tttgaagtct ttattagaca tcgaaagcat tggctttaat tttggatata cttcgtctac
    15961 ttttggttcc atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa
    16021 ctggcagata tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat
    16081 catccctttt gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc
    16141 aagcaatgct tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc
    16201 tcctggttta tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga
    16261 accgaactct tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg
    16321 tgctacagcg acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc
    16381 ttctttttgc catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc
    16441 tctgagtata tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc
    16501 atccctaggc acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga
    16561 tttcggctga tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa
    16621 gccagaagta tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg
    16681 ttccgccgtg gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc
    16741 ttcttttaat gtcatggtct tcctcgctta ctgttaacgg ctgtataacc cataatatag
    16801 tttttctttt ttcagctgga caaaatggat ctgctgatcg gcaatcttct tttcttccat
    16861 ccattttttt cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt
    16921 atctaatgca ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct
    16981 tacctcttcc catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag
    17041 atttttttgc tgtgtgtacg tgaatgtttg cgggattttc ccaggcctgg caatttctac
    17101 tagacgatta taaaacatat tcattaattt gctgttggca ataaattgac ttttataata
    17161 ggccgactgg atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca
    17221 tgctttgacg gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg
    17281 agaaaggaac acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa
    17341 caaccatttg cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat
    17401 gaataattta gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc
    17461 ctgctggctg atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa
    17521 attcatagcc acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta
    17581 tctgtacgcc gaaaaacagc ataatttttt tcatatctct tataaaaaat gcaaggcctg
    17641 ctcctcaagc tagaccttgc acttttcagc tttcttttat ttgtgacaaa ccttcatgga
    17701 tcgtttcaat attccatttc atcatctgat agtaagaatc tccttgttct ccttcttttg
    17761 ctatggaatc agtgaacagt ttatcataaa ttttcaaacc cgtttctttt gctacccgtt
    17821 ccatgcttct tgagtctaca ctggtttcca cgaataaaac cggtacttct tttgctctta
    17881 tctgatcaat gatcgctttc atttgatcag gcgtaccttg actttctgta ttgatctccc
    17941 atatataagc tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg
    18001 ttactaataa ttttttgttc tcttcgatag aagcaaaact ttccttcgct tgtgtatcta
    18061 attcttttag tttttccaca tatgcttctg catttttttt atagagtgtc gcattttctg
    18121 catctttttc agagaatata cgtgcgattt cctccacata ttttattccg ttagataggt
    18181 ctagccaagc atggggatct gcttttgtat gttcttcacc gctagttaaa taaagaggtt
    18241 ctacattttt gctaactgca aaataatctt ttccttcttc ttttttagcc gtttccatca
    18301 agttatcgaa ccagctgtta cctgtttcaa gattcaaacc gttgtataaa ataacatccg
    18361 catcgcttgc ctttttgatg tcttctggta gtacttcata ttcatgcgga tctgttccga
    18421 ctggtacgat actgtggata tctatttgat ctgttccgac ctcttttgcc atgtccgcca
    18481 agatcgaatt ggtagccacg actgacaact tttctttact tccttcatcc gcttggtcag
    18541 aagcctgctt tccgcaaccg gctaatacca agaaactaac tgccaagatt aaaaagagtg
    18601 atttttttgt tttcatctat ctgcttcctt tctgaaataa aattcctttt gttggagaaa
    18661 ataaaaaagc aaggataaaa aatgcggtac atgctaacac gatcgtagct cctgatggcc
    18721 aattgtaact ataactgaaa aacacaccga caactgcact tagcataccg attcctgcag
    18781 ctgtaatgat cattttcagt aaatggttcg ttagcaagta agccgtggcg gctggtgtga
    18841 tcaacatggc aatcaccaag attgttccta ctgtctgtaa actgactaca gccactaatg
    18901 tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt cagcccataa gcttttgcca
    18961 ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca aatatcaaga
    19021 cgattccact aacgagtgct gtcagcaaca aatcgctttc cctaacagct aagacattcc
    19081 caaataaaat atgatacaaa tctgtcgcac tctgggcata tgaaatcaag ataatcccta
    19141 atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc atttttcaat cggctccgct
    19201 gagtcacgaa accaatcact gctgctgtta gcatcccaaa agcagtggct ccaaagatgt
    19261 aactaaaacc aaacatataa gaaatggcta cacctggaag aactgcgtga gagatagcgt
    19321 ctcccatcaa agacatccct cgtaaaataa taaaagaacc gatgactcca gaagcaagtc
    19381 ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg ataattcatt aatccatcaa
    19441 taaaggattg gatcattttt tctctcctcc ttttactaaa tgtctgatga tctcaccata
    19501 ggccaattga aggttcttcg atgtaaagac ttcttgtact ggaccggaag cgatcagctg
    19561 tttatttaaa agaataacct catcgaaata ttcttctact ttatgaagat catgatggac
    19621 gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc
    19681 acttaacgca tcaatcccta cgaatggttc atctaaaaag atccattctg ctccttgggc
    19741 tagagctctt gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc tgatctgtct
    19801 tttggcatat tcttccatcc ctactttttt caaagcttgt cttgcacgtt ctttttcttt
    19861 tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa
    19921 tacatcaatt ggaaaggaaa gatccaattc acttcgctgt tccacgtagg ctattttttt
    19981 acggactgag tttaccggct gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa
    20041 ctctagcaaa gatttcataa atgttgattt tcctgcaccg tttggtccga taataccggt
    20101 aattttttgt ggatttatcg tcaagttgat agttttcaaa acagttttcc cttgataagc
    20161 ggctgaaagg tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt
    20221 ttacctaact ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat
    20281 gatgaaaaag tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca
    20341 tcaagagttc aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt
    20401 ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga
    20461 agtcttagtg cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg
    20521 tttatgggtg cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg
    20581 attgcaggat tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat
    20641 ccttcgactg gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac
    20701 ttgaccgctt ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct
    20761 gtaggatctt atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt
    20821 gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa
    20881 ttttggtttt cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg
    20941 gtaatcttat ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa
    21001 aatggtggat tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt
    21061 gcttcctatc aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa
    21121 aagacgatca ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt
    21181 gctattttta tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct
    21241 tttgtacaaa ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc
    21301 gtgattacag cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc
    21361 tatacacttg ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt
    21421 gcgccttttt atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat
    21481 tatcttctac caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca
    21541 agtattttgc ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa
    21601 aagaagcacg cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg
    21661 aattatgtga cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa
    21721 acgagaatct cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt
    21781 gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt
    21841 cgttcttttt tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata
    21901 attctccatg ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc
    21961 ggctatttga tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc
    22021 gctgttggta ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc
    22081 agaagcttct accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt
    22141 tgactctgct tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg
    22201 attcgtataa ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt
    22261 ttctgctgcc tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag
    22321 ctcacgtgct ttttggttag caactgctga taaaccagct attccctctt gatttccctg
    22381 aatttctgtc tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata
    22441 atcatttacg attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt
    22501 ttgtgtctct tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt
    22561 cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag
    22621 acccgcaact cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat
    22681 ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca
    22741 ctcattttca tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac
    22801 gcttcatatt cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt
    22861 ataagaaaat ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc
    22921 tgcattgttc gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg
    22981 tcttgatatt ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc
    23041 ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg
    23101 cctcagtcag tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct
    23161 gattttcctg tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt
    23221 ccaatgagag atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat
    23281 acataaattg tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc
    23341 gctgatacgg cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt
    23401 ctttttccgc cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg
    23461 ctgctcgttc caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt
    23521 aaaacggaaa ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta
    23581 tatctatttt tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag
    23641 cagaagcttc tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga
    23701 tgataagtgt acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac
    23761 cgccacaagt ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag
    23821 aaactgcggt ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat
    23881 tttcgttttt catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca
    23941 ttcggctaaa tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt
    24001 catccgtcag agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct
    24061 tgatactggt acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta
    24121 aatgctgggc gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga
    24181 ctgtttcaac agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat
    24241 cttttaaaag gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca
    24301 aggtttcttc cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag
    24361 aagagaccat cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag
    24421 cactcccacc aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa
    24481 acgtcgagtc tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat
    24541 ttttcacatg ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa
    24601 tagcaggacc aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca
    24661 attctttttc tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc
    24721 ctccttactc ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta
    24781 aaaaggaagc gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa
    24841 ccggtttact tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat
    24901 gatccaacgt gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt
    24961 aagcacttat tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac
    25021 gaatgcaaaa atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat
    25081 tatttttgat cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt
    25141 caaaccatgg ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac
    25201 caatttcttt ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt
    25261 tttcatctta ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc
    25321 agctttgagt acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc
    25381 ttctctaggt gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta
    25441 tcttgttact cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc
    25501 tgctgttatt tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac
    25561 tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca
    25621 aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgtagc
    25681 aacaactgcc gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg
    25741 gatcgttggt ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct
    25801 tacacgcgtc atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc
    25861 ttactttttc tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt
    25921 gttctacttc cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt
    25981 tgaatatggt caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc
    26041 tttactagat aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc
    26101 ttctgggatg actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt
    26161 caaagcttat gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc
    26221 ttggaagatt acattgacag agaaaaaaca tgcacaaatc gttgaggaat tggaaaacaa
    26281 attggctcca gctgatacaa tggaagatga acttcacgat gcagtagttc cttcccatta
    26341 agaaaggatc ttgaaaatga agattacaga aaaagatttt ggccaaggtt atcacttgat
    26401 aaccttggag aacaaaaaca agctagcact ttctatctct gatttaggtg ctagaatcgt
    26461 cagtttaaaa tcaaatgatc gagaattagt tttgggattt gacaccgctg aagaatatat
    26521 cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg
    26581 ccgcttttct ctcaatggaa aaacttacca attagccaca gatccaaaaa ctggacataa
    26641 tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg
    26701 cgaaaatgaa gcttctgtca tttttactac tacaagtccc gacggagaac atggttttcc
    26761 aggtacaatg gatgtggaaa tccgctatac tttgacaaaa gataatatct ggcgagtgac
    26821 ctctcgcggc accagtgacc aagatacttt attcaatccg acaaatcatg tttacttcaa
    26881 cttgacggga gatgcttctc agtcaatcga tcaacatgag ctttggctca atagcgaagc
    26941 ttatgctcct ttgcgaacag atagtattcc tataggggta aaagaaaatg ctgcaggaag
    27001 tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg atttggatca
    27061 aaaaaatctt gttgacggaa tcgatcatcc atttttctta aaagaaaccg gcttgggtaa
    27121 agaagccgct cgcttgacta gccccgataa aagaatccaa gtaggtattg ctacagatgc
    27181 ttctagtgtg gttattttca ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg
    27241 aaaactggca catcacggtg gcatcacatt tgagactcaa acagctccag gtgcagagcg
    27301 cttctcagca tttggttcga ttcatttaaa agccggatca gtatttgaaa cagtgacaga
    27361 attcaaaatc aaaacaagaa aggaataact catcatggaa atcaaaagaa gcttaaacac
    27421 aaaatttgcc gaaacattcg gacctaaaaa aactacacaa tacttctcac caggacgcat
    27481 caatttgatt ggtgaacaca cagattacaa tggaggccat gtcttccccg cttccatcac
    27541 ttatggaacg caaggtgtag cagcacctcg agacgataat aaaatattag tttattccac
    27601 aaactttgac gatatcggtg tcattagttt tactttagat gagctagaat atgataaaaa
    27661 agcaaactgg gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag ctggacactc
    27721 aatcgatcat gggtttgagt tgttagtgga aggaacaatc cctaatggtg caggactttc
    27781 cagtagcgct tcattagaat tattagtagg tgttgtatta gaagatctgt ttgatttaaa
    27841 aacagatcgc ttggaactag tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt
    27901 caattcaggt atcatggatc agtttgccat cggcttcggt gagctagatc atgcaatctt
    27961 attagataca aatacgctga aatacgaaat ggttccagtg aaactagatg gttatgcaat
    28021 cgtcatcatg aatacgaata agcggcggga attggcagat tcaaaataca atgaacgccg
    28081 tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact ttgcctatct cttctttagg
    28141 agacttagat gaagaagaat ttgaatcaac tattgaccaa atcggcgatg atacattgat
    28201 acgcagagct cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt
    28261 agaagctggg aacttagaag catttggtca actgttaaat gattctcacc actctcttcg
    28321 ctatgattat gaagtaactg ggatcgaatt agatacacta gtagatgctg ctcaaaaaca
    28381 agaaggtgtc ttaggtgcac gaatgacagg agctggattt ggtggctgtg cgattgctct
    28441 tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt
    28501 tgtcggctat gctccggaat tttatgttgc acacattgga aatgggacaa caaagatcga
    28561 tccagaagct gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa caaattgata
    28621 aaaataacct ctcagcaatt tccaaatgtg agccttttgc tcgtatttga aaattgctgt
    28681 tttttattga cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg
    28741 tgggcgaatc aaaatccggt atttgtttat gaaaaagaac cttcaagaga tattttatgt
    28801 attgactgca aatcttttta cgcctccgta gagtgcgttg aaaaggatct tgatcccctt
    28861 acaactaaat tagtagtcat gtcctatccg tccgattcga cagaaacacg gggtagcgga
    28921 ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt
    28981 gccagagatc ttcccttccc ctatccaagt gatttatata ttgttgcccc aaggatggcc
    29041 tattatatgg aaaagaacag agaaatcaat tctatctata aaaaatatgc agacgaagcg
    29101 aaccatcatg tctattccgt cgatgaaagc ttccttgatg taacaaatag catggcactc
    29161 tttggtaaaa caaatgcgta cgacatggct aaactgatcc ataaagatgt gtttgagcag
    29221 accggaatct acacgactat aggaattggc gataacccgc tacttgctaa actagcctta
    29281 gacaatggct caaaagacgg aaagcaaatg tttgctgaat ggcgttatga agatgtacca
    29341 gaaaagttat ggaaaatctc agatatcact gacttttggg gaatcggcaa acgaacagca
    29401 aaacgtctga aacaaatggg tatacattct gtttatgatt tagctcacgc ggattattat
    29461 cacttgaaaa gcaagctagg ggtcatcggt actcagcttt atgcccatgc ttggggcatc
    29521 gatcgaagtt ttttgggaca agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa
    29581 atcctggata gagattatac aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc
    29641 gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa cacaagtagt cagcttatgg
    29701 gtaggatact ctttaggcta tcttgatcca aatggcaaga ccgggtttca ccagcaaatg
    29761 aaaatcgaag cgacaaatgc tagcagagat ctagcagatg ctttattgac cattttccac
    29821 cagcattacc actaccagga tattcggcgt gtaggcgtga actgttccga cctcgtatac
    29881 gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa
    29941 gttgattttg ttgtcgatac gattagaaaa aaatacggct ttcaatcaat catccatgcg
    30001 catagtctct tgcaaggagg acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca
    30061 ggtggtatga gtggattaga aggtggaaaa gatgagacgg acagcaaaaa aattcattga
    30121 ctataacgag tatcgagatc ggccatttgg attaaaatgg gggactgctt ttgcgatgga
    30181 agaactagtc aacggtatca aaaaaaatga agaagaagca cgcaaacagc ctcttcctaa
    30241 acaacaaatg acattgcaag aaatcgatag tatactgaca gaagcctttt tatctcgtaa
    30301 agcagtaatc attcagctca atttaaaaga tgaatttgga agactcttgg atgaggtcga
    30361 agggcacttc attggtgaag cctatgaaga ttactttatg attgaccatc agccgatatt
    30421 gtgggaagac gtacggtata ttgagttaaa ggaaacaaaa aaatggtttg tgacatgaga
    30481 ggttgtgaca gaagtgatca acttcaaaaa ataagaaagg atttttcgaa aattgcttct
    30541 taaatttttg tggaattttg gcttattttt cgaagaagca acttctggaa caacgtatat
    30601 tagatatcag aggttgtgaa aaagtcgctt agttttgagt aacaagccaa ggcgctgatt
    30661 tcacaacctc tgtctatcta tccatctatt cggtaagatt catgcatgac gtcttcttta
    30721 tttagtatgt agagcccttt ttcttctgac cattcaacat aaatcagctg ttttacatct
    30781 gcatagcctt ctttcttcaa gttctccata agccatgctt cagtcaattg attggactga
    30841 agcactttat gctgaatttg cccttcatct acaagcagga tcgataaagc tttgtcttca
    30901 tctgattttt tcaatacact catttgaccg gcattctcta ggataacatg tttggcattt
    30961 tcaagagaaa agcattcttg ctgtcttaag attgctcgca actgctccat ctcgatatgg
    31021 ttatttgtca actctgtcat gttgatgacg ccgtctttga ttagaacaga tggctcgcct
    31081 ttgacccaac gattgacctt ttcatttttt tggacgatac gttcgatacc ataaatcatt
    31141 actgcccaaa gcgcaatggc aaataggaca tgccctacat ggacattgtc atcataggtt
    31201 gattcttcta agatcccacc taaaacaagc gtatagacta aatcaaacgg tgtgatttct
    31261 gacatggatt tcttcccaag caaacgtacc actaataata aaccgatcaa cccgaacacc
    31321 agtttcattg taattgaaaa aatcattttt tctcccccta actatctgtt aattaaatta
    31381 tatatgcttt ctcacgaaca cgcatggaat atacaaggaa gaaatgtatc cttttactaa
    31441 aaaaaggact gtgaca
//
LOCUS       DAPBBG010000032        31160 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPBBG010000032 DAPBBG010000000
VERSION     DAPBBG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.032"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(189..2261)
                     /locus_tag="RD477_001667"
     CDS             complement(189..2261)
                     /locus_tag="RD477_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7634027.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2194..2571)
                     /locus_tag="RD477_001668"
     CDS             complement(2194..2571)
                     /locus_tag="RD477_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDT7634028.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2700..3278)
                     /locus_tag="RD477_001669"
     CDS             complement(2700..3278)
                     /locus_tag="RD477_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7634029.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3410..4702
                     /locus_tag="RD477_001670"
     CDS             3410..4702
                     /locus_tag="RD477_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7634030.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4762..5880)
                     /locus_tag="RD477_001671"
     CDS             complement(4762..5880)
                     /locus_tag="RD477_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDT7634031.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5894..7315)
                     /gene="arcD"
                     /locus_tag="RD477_001672"
     CDS             complement(5894..7315)
                     /gene="arcD"
                     /locus_tag="RD477_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDT7634032.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7561..8682)
                     /gene="mnmA"
                     /locus_tag="RD477_001673"
     CDS             complement(7561..8682)
                     /gene="mnmA"
                     /locus_tag="RD477_001673"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDT7634033.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9086..9685
                     /locus_tag="RD477_001674"
     CDS             9086..9685
                     /locus_tag="RD477_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDT7634034.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9823..11109)
                     /gene="efmA"
                     /locus_tag="RD477_001675"
     CDS             complement(9823..11109)
                     /gene="efmA"
                     /locus_tag="RD477_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDT7634035.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11380..11724)
                     /locus_tag="RD477_001676"
     CDS             complement(11380..11724)
                     /locus_tag="RD477_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDT7634036.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11861..13006)
                     /locus_tag="RD477_001677"
     CDS             complement(11861..13006)
                     /locus_tag="RD477_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7634037.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13186..14160)
                     /locus_tag="RD477_001678"
     CDS             complement(13186..14160)
                     /locus_tag="RD477_001678"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDT7634038.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14240..15115)
                     /locus_tag="RD477_001679"
     CDS             complement(14240..15115)
                     /locus_tag="RD477_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDT7634039.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15245..17152
                     /locus_tag="RD477_001680"
     CDS             15245..17152
                     /locus_tag="RD477_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDT7634040.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17693..20971)
                     /locus_tag="RD477_001681"
     CDS             complement(17693..20971)
                     /locus_tag="RD477_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDT7634041.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21242..21787)
                     /locus_tag="RD477_001682"
     CDS             complement(21242..21787)
                     /locus_tag="RD477_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7634042.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21799..22491)
                     /locus_tag="RD477_001683"
     CDS             complement(21799..22491)
                     /locus_tag="RD477_001683"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDT7634043.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22510..22794)
                     /locus_tag="RD477_001684"
     CDS             complement(22510..22794)
                     /locus_tag="RD477_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634044.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22807..23370)
                     /locus_tag="RD477_001685"
     CDS             complement(22807..23370)
                     /locus_tag="RD477_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7634045.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23598..24260
                     /locus_tag="RD477_001686"
     CDS             23598..24260
                     /locus_tag="RD477_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDT7634046.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24384..25580
                     /locus_tag="RD477_001687"
     CDS             24384..25580
                     /locus_tag="RD477_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDT7634047.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25681..27057)
                     /locus_tag="RD477_001688"
     CDS             complement(25681..27057)
                     /locus_tag="RD477_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7634048.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(27094..27960)
                     /locus_tag="RD477_001689"
     CDS             complement(27094..27960)
                     /locus_tag="RD477_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDT7634049.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(28049..29074)
                     /locus_tag="RD477_001690"
     CDS             complement(28049..29074)
                     /locus_tag="RD477_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7634050.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(29092..29766)
                     /locus_tag="RD477_001691"
     CDS             complement(29092..29766)
                     /locus_tag="RD477_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634051.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29947..30843)
                     /locus_tag="RD477_001692"
     CDS             complement(29947..30843)
                     /locus_tag="RD477_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7634052.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(30917..31042)
                     /locus_tag="RD477_001693"
     CDS             complement(30917..31042)
                     /locus_tag="RD477_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7634053.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         9006 a         6661 c         5574 g         9919 t
ORIGIN      
        1 tttttttaaa tgaaaaagga ctgtgacaaa agtcttgtca cagtcctttt ttccgaataa
       61 atggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaag
      121 caattttcgt aaattccttc ttatttcttg aagctgacca cttctatcac aacctctttc
      181 atatcttatt acttcttcat tgctgctttt agtgctgctg ctaatgggct ttcttctggt
      241 tcttctgctt gtgcatattt tttcagcaag cgtcgttctt catgtttggt cattttcttc
      301 ttctgctctt ttttatcaag caatttttct gtcgttccat cgtatttaca acggaaataa
      361 gcaccgtttt taccttcgat gatttccatt ttccggtgac attgcgggca tcgatgatta
      421 gagacttttg gatcttttct tctgcggtag ttgcaatctg aattggtgca gacatagatt
      481 ttgccgtcac gtgtgttttt ctcgcgcaga ttggccccac attctgggca tttcttttgg
      541 gtaatcgaga aatcttgata ttcttgtttg cttgatttga tctcattcac caagcgtttt
      601 gtttcttttt cgattccatt caagaaggtt cctgcttgtt tctttcctgc agcaatagct
      661 tcaaggtctt tttcccagct ttctgtcaaa tctggtgtta ctaaagaggg atttaccaat
      721 actaataatt gcttaccttt aggagatact tgcagtcctt gaggggtacg ttccatcagt
      781 tcagatttga ttagtttctc gataatctct gcacgcgtag ctggggttcc cagactgtat
      841 ttttccattt gaccaagcaa gcttgcttct gacaatggtt taggcggtgc agatagttct
      901 tttttgatgg cgaaatcggg attgattctc attccttttt cccatttgac tgtttccgtc
      961 tcagtttcag tttcgccttt ccatcctgct tggacgacac gattttgccg aaaaatgaat
     1021 cgttctttac caaattcaac cgtaactttt gtttggctga ctttatgggg ttgagcaaac
     1081 agtcctaaaa aacgagagac aatcatttga tagatttttc gctcttcatt acttaatttt
     1141 tcataacgtg ctctgttttc agttggcaaa agtgcatggt gatcggtcac tttactgtct
     1201 tggaatactt ttgtctgttg gacgatggct ccgtttttca aatactgctt gacctcagga
     1261 gcaaagtcgc tgactgcttg caatcgttct ttcatcgtac ttttcatgtc atttgtcaga
     1321 tatttgctgt ctgtacgcgg ataagtaacg atcttatggg tctcatacaa actttgtaca
     1381 agacttaatg ttttctttgc agaatagcca tagcgttgat tggcttctct ttggatctct
     1441 gttaaatcat aaggcaatgg cgcagattcc gtttttaatt tttcttggac ttctgtgaca
     1501 gttcctttgc ttgtactgat caattgaaca agtttttgtg cttcttcttg tgtttttaaa
     1561 gcaaattgat ttttttgaag cattttggct tgttcttttc cagcttgcaa cgtgatcgtg
     1621 aaatacgttt gtggacggaa ctgctcgatt ttcttttctt ggtctctcac taatgctagt
     1681 gtcggtgtct gcacacggcc tgctgataaa ttatccgcgt acttcacagt taaggctctg
     1741 gtcacattca atcctaccag ccagtccgct tttgcccggg caagagcaga ttcatacaat
     1801 gcatcatatt ctttcgcagg tcttagttgc ttgaatccag ttttgattgc tttatctgtt
     1861 tgagaagaga tccataaccg cttcactggt ttattgaaat gcacatactc taagatccag
     1921 cgagcaacaa gttccccttc tcttccggca tctgtcgcaa tgactgcttc tgagacatct
     1981 tttcgctgag ccagctgctt gattgctttt aattgatgtc ctgttttagg aagtggtttg
     2041 attcctagtt ttttagggat cattggcaac gtgtccattt gccagctctg ccattcctta
     2101 ttcagatctt ccggcatttt caatcctaat aaatgaccaa gggcccaagt aacgatcacc
     2161 ttgggtcctt cataataatt tttgtgcttt tgattagcac caagcacctt gcttaggtct
     2221 ttcgcaacgc ttggtttttc cgctagaatt agttgtttca taaaattcct ttctgctttc
     2281 tactgtgatg ggctgatgat ttttcaacaa cgcaagtccc ttgtattgct gtaaatcatc
     2341 gtcacactct tcacaccagc gctcatctcc gtcattccaa aaggcgctga gaaagttact
     2401 ttttgcattg atatattcat aattttgatg gatttcgttc cattgttttt cgtacgcttg
     2461 tcgtgcttct tcgaaagact cgaatgtttc ttcttcttca atatcttctt cccagtcttc
     2521 aaaaaaccac caaggctcat tgtccccgaa catggtaatt acttgataca tactcgtgct
     2581 ccttctttca gcttttctag actcatctta gtcacttttc aagaaagttg caagaatttt
     2641 ctcttataaa agggtcattt aatgaacatc ttaaaattgt tacaaatgaa tctgattgtt
     2701 taaggcaaag tagcgataaa gttattgatc atccaaaaat agctgtcttc tggatcgatt
     2761 gttggttctt ttgtaaagta tcctaaaaaa cgtaaggaaa gataaccgtg gatcatacta
     2821 cgaagttccc tgcttcgatg aactttttct tctttcgtta attgaaaagc ctcaaatatc
     2881 tgcaaaatga tctgattcgt ttcatggatt ccatctaata aaacagcttc attggtatgc
     2941 ggtacactga tcagtaactc atacactgct tgatttgaaa aagcaaagga tcgataagtt
     3001 tctgcataga tctgcagtgc ttcctgacca ctttttccca caagagcccg tcgcagctgg
     3061 tcattaagtt cctgcagaag tttcgctgtt agcgctgttt tgacatcacg aatatttttg
     3121 aaatggttat acagcgaggg agccttgata tccaagcgtt tggctaaatg ctgaaaggtt
     3181 atttgctgaa catccatctc ttctgctagt tctcgaaagc agtcgatgat tttctcttga
     3241 gacagatttc tttttttcat ttccatgttt cctctcataa aataaatcct tcactctttc
     3301 agtttaccac gaaaaagaaa gagaaaaaag agactttcat tgataaacta catattgtag
     3361 tttataataa agagctgtag atgaaaattt tcagaaagaa ggagtctaat tggctgcaag
     3421 aataaaggaa aaattattta ggaaaagaga aacttgggaa caaaacttgt ttgtcttatg
     3481 gtttggaaca tttatggctg gtatcggatt tagtttagtc atgcctttta tgtcacttta
     3541 catagatacg ttaggtaatt atagtacgtc acaattgaac ttttggagcg gattgacttt
     3601 ttcatctact ttcttggtga ctacgcttat ctctccttgg tggggaaaat tagccgacca
     3661 aaaaggaaga aaattgatgc tgttgcgtgc ttcattagga atggcaatcg tcatttcttt
     3721 gatgggttgc gtgactagtg tgtatcaatt agtcgcattg cgtctgcttc aagggatttt
     3781 ttctggttat atcagtaatg ccactgccct tgtcgcaact gggacgccaa aagaaaaaag
     3841 tggtcaagtt cttggaacct tagcaactgg ttctgttaca ggaacattac ttggaccgct
     3901 tcttggcggg atctctgctt cagcgtttgg ctatcgccct acctttttca ttacagggat
     3961 catcttattt ttcgtcttct tgttgagtct gtttttcgtc catgaaaaat ttgtcccagt
     4021 gaaaaaagaa aacatggttt ctgccaaaca gattttcaaa gacttgaaat atccacacgt
     4081 cgtcatcggt atgttcgtca caacgatgat catccaagca tccaacaatt ccattagtcc
     4141 aatcatcagt ctgtacattc gccaattgct gcatggacat gggaatgtta cgttgatcag
     4201 cggaatcatt gcttcgatac ctgggattgc cactttgata gccgctccaa gatttggtcg
     4261 cttaggtgac aagattggga gtgaaagaat acttgcgatt ggtttgggtt ttgcgattct
     4321 ggtttatatc ccaatggcat tcgtcacgaa tgtttggcaa ttagctgcct tacgtttttt
     4381 gattggtatc tctgatgctt gtctgctccc tgctgtccaa gcattgatca caaaatattc
     4441 gcctcatcaa gctgctggac ggatcttcag ttataaccaa tctttccaag caactggtaa
     4501 cgtagtaggg cctatgatcg gctccagtgt ttccagtgca tttggttatc gcggcgtctt
     4561 tctttcgact tctcttcttg ttttggtcaa cttcttgctt gtacgacaca atacacaaga
     4621 gatccatcag caagaaactg cagaagaaaa agcttcagga gtctccatag cttcagggca
     4681 ctctccttct catagacaat aagcgtaaat gaaaaacact gcttgaacag ttgtcacaga
     4741 ctgtccttgc agtgtttttg tttaggcctg ttttttcaag aactcagata agaggaataa
     4801 tcccttttcc aaggtttctt ttttacaaca gtaacctagt cgggcatgct tcggtaaatc
     4861 aaaggcactt cctggtacta atagtactcc tgtttcttct aataaattga tacaaaatgt
     4921 ctggtcatct tcttcgatgg ctaatttaat gaaagaagta gagacgtaat tgggagcgac
     4981 aagttcaact ttcggttcat tggctaccca ttgttttaaa atcacaaggt tctctgtaat
     5041 gatctttttg tttcgcgcca atattttttc tttattcttc aatgcatgta cagcaagatc
     5101 atctgacagg actccgccgc aaatcatcgt gtagtctcga tactttcgaa aaacttctgc
     5161 caactcaggt ccagtagctg tccagccaat acgaatacct ggaatggagt aggttttgga
     5221 tagggaattg gttgctatac ctttttcata gacatctacg atcgacaaga attcaccttt
     5281 atccgttaaa ggcgcatata cttcatctat caatacatat gcatctactg tacgagcaat
     5341 ttctgcgatt ttttctaaag ttttccgatc tagcaatgtg cctgtaggat tgtttgcact
     5401 attcaaacag atgatttttg tttcaggttt gactaaggct tctagctgtt ctaaatcaaa
     5461 ctgccaatct tcttcttcct tcaaatgaac aaagtcaaca gcggcaccta aagatttggg
     5521 gatgtcatat aattgctgat aagtaggaag catcgaaaca acatggtctc ctgcttccac
     5581 taacgcgtat agagctaaaa gattagcacc agtcgctcca ttcgtttgta agatgttttc
     5641 cggatcgacg gtttgataaa gagaagcgac ttcttgtttg aaaacatctg atccttcgat
     5701 ccagccatag tccaatggtt cgtttttgcg tttttcgaaa aatttactta cgctagtccc
     5761 atctaagcca acgagttcgt ctaatgtcaa tgcttcgatc gagctttgtg caatatcata
     5821 taccgctttc ttttcccatt tatttagcca ttcttctaca ccgaaatttg cgattttcat
     5881 gtttctttcc cccttatatc ttgatccagc ctacagcaag caacccgatt gaaagcagtg
     5941 cggccacaca aatcagccca atcattactt tttcttttcc cgtaatttga tgtttggttt
     6001 cacgacaagc gatccagtag aagcaaaaac caggaagata ggcaatacta cataaaagga
     6061 cataagaaaa tcctgctaaa atgatgccca tcaattcaaa aactacagca atcccaccga
     6121 tcactacttg ttttttgatt ccttctttta aagaaaactg gacttgatag atcccgacaa
     6181 gtagataaca aatcaaaatt gccgctgtac ataaagaata ggcaaattga taggcataat
     6241 cagtaactaa gaaagtcaat aagaatagat tagtcaagat tgcagtaaca actaacgaat
     6301 aagttggggc atttttttta ttcgtttttc cccaaccttt cggcaacaga ccgtctcgag
     6361 ccatcaacat cgttgtttct gctgggagca tcgtccaaga caaccaagaa ccaatgatcg
     6421 aaataatcaa gccgatattg atcagcgctg ctccccaatt tcccacgatt ccttttaaaa
     6481 tatttgccat agcaggttgt gaaatagttg ctaactcttc ttgagataag acgccgtatg
     6541 gcaagatcga agcaaggata tagataaaca ataaacaaag cagaccaatg atcgatgctt
     6601 gctgggcttc tgaacgtttc ttcgcgcggt tggctaaaac acttgcgcct tcgatcccta
     6661 caaagaccca catcataacc atcaaacaac ctttaatctg caaccagatc gttccttgct
     6721 gccctgttcc attcactaga ttatcagata cgttacccca aaaatcagca gtgaatatat
     6781 tcactttaaa tgcgataaat acaaatatta aaaataggaa taatggcact aatttacaga
     6841 tcgtcacgat tgtattgatg acgctcgctc cttcaactcc attgtttact aataaagtca
     6901 gaccccaaat aaaaatactt gcaaataaaa tagaaggaac attttgtcca cctttgaata
     6961 tcgggaaaaa ttctcccatg gcgctcatca gcatcgttgc aaaagcaaca tttcccagcc
     7021 acgcagacaa ccagtaaccc caaccactga tgaagccacc taatttacca aatgaagctt
     7081 ccgcgtaacc gaaaatacca ccatctagat ctggacgctt ttctcctaaa tgattcagcg
     7141 ataataccaa agctaaaata ccaatcccaa cgattatcca tgaaaggatc gctggccctg
     7201 gagctgcaga actagccaga tcacttgtga tcccaaacac accgcttccg attgacgaac
     7261 tgacaaccag agcaaccagt ccccatttgc taatacccgt cgatttcttt tccatttcca
     7321 tcaccctcac ttataaaatt gaacaaatca cttgcaaaag attcccttct attttgcaac
     7381 tgctcttgct taacaaaaag caactttatt catattattg gatattatga actttttaca
     7441 caagtatttc aagtataaaa tgaataaaaa ttaattttat tgcaaaaaaa cagctatgct
     7501 atccaaaaga aaaggtgttt cttttgttag ccatagctgt tttgattcat ttaatatcgc
     7561 ttaaacgtat tgtaaaactt ttgtttcttg atatgcgtgg tcgatcaaac caccacccag
     7621 acattccatt ccatcataaa atacaactgc ttgtccagga gtgattgcac gtacaggttc
     7681 gtcgaatacg acttctgcac gattatcatc caataagcga actgtcacag gaacgtcttg
     7741 ctgacgataa cggaatttcg ctgtacactt gaattctttt ggcattggtt cattcgtcgt
     7801 aaagtggatt tcacttgcat ccaagcttgt ggcatacaat gcaggatgat ggaacccttg
     7861 ccccacatat aatgtgtttg tcgctagatc ttttcctaca acaaaccagg gttcttgcga
     7921 atctcctccg ccaccgatcc ctagtccttg acgttgacca atcgtataat acatcaagcc
     7981 agcatgttgt cctttgactt caccatcaag agtgaccata tttccttttt ttgctggcag
     8041 atagttgctc aaaaattgtt tgaagttctt ttcaccaata aagcagattc ccgttgaatc
     8101 ttttttctta gcagtcgcaa ggcctgcgcg ttcagcaatt gcccgtactt ctgatttttc
     8161 catgccgcct agagggaaca tcgtttttga aagctgttct tgtgataatt ggctaaggaa
     8221 gtaagtttga tctttattat tgtctactcc acgaagcatg tggaccacgc cgttttcatc
     8281 gcgagtgact tgcgcataat gtccagtagc tacataatcg gcgccaagat ccattgcata
     8341 atccaagaat gctttgaatt tgatttcttt gttgcacatc acgtctgggt taggtgtacg
     8401 tcctgcgcgg tattcggcta ggaaatattc aaatacacga tcccaatatt ctttttcaaa
     8461 attcacagag taataaggaa tcccgatttg agcagctact ttcgctacat ccttataatc
     8521 ttccgtggcc gtgcatacac cgttttcgtc cgtgtcatcc caatttttca tgaatattcc
     8581 gatcacgtcg tatccttgtt cctttaacaa aagtgctgtt acagatgagt cgacaccgcc
     8641 gctcatccct acgacaacgc gtgttttgct gttgtctgtc atgatatcac catcatttca
     8701 attagattct gaaacatcta cgagttgaag gtcgcaactt ttcagtattt tacattatac
     8761 gtcatatttc tgaaaatttc atcccttaat tatcaagtcg taaaaatact gttctactgg
     8821 acctattcag tacacaaaca tgatagtcaa ccaaaaaatc actcatcttt tgagcattcg
     8881 atataaaaaa ttggcatact cataaaaagg gccagccttt cggcaataag gaataatttt
     8941 ataaattgtg aaaaactatt tttcaaaatt gcttcttatc gcttaaagct gcacgtctca
     9001 tttacaacct tttcttttta gatgttatga gttgctcaga taagtgattt tgtttactct
     9061 aagaaaaaag ggaggcaaaa gatacatgaa ttactttatc agtgatatgc actttttcca
     9121 tgaacgctta ttaggaaaaa acgactttgc tccgcggctc tttgcaaatg tggatgaaat
     9181 gaaccaacag ttgatcacta gctggaatgc agtaataaaa gaaaccgacc atgtttatca
     9241 tctaggcgac cttgctatgc atccgcaata tgaaaaagca aatgcagaaa ttttggaact
     9301 ggtcactcaa ctgcaaggaa caatccactt gatcaaaggg aaccatgaca gtcgcgcttt
     9361 tttccgttat ttagaaaaaa atgatcctag gcttactgac ggaagtccca aattttattt
     9421 ttatgatgta ggtgccatcg ttaaattcga ccatcaacaa tattacttga ctcattatcc
     9481 gatgttgctc gggcctaacg acactatccg caatcttcat ggacatatcc atcattacag
     9541 cgtacctatc gagaacgatg tcaatgtagg cgtagacgca ccggaaagag aactcttaaa
     9601 ggaaaaacta ccatttggca ctccgttatc tgagcaacaa atcaaagaaa tctatgaagc
     9661 aaagaggaag tacgataggt cgtgaaaaaa ctgccctata tcaagtatta aacaaataaa
     9721 gataattact catatttttc atttgagtag gcttattgcc acaggaacat taggatcaga
     9781 aatggagttg taacaagact tctgtcacaa ctccattcca tactattcgt ctttttgctc
     9841 atggtgttct tttaccagtt ttttctgtaa ttctttatca taatttcggg cagctgacgt
     9901 taaaaataaa acgattccgc ataataaaat cccgattccg gaaaacaaga acattttttc
     9961 tacccctaaa cgatcagcta atggtcctgc aaataataat ccaattggac ctgccaggtt
    10021 catcaaagag ttgaagttcc ctaagacacg acctaaaaca ttcgattcat aactctgctg
    10081 gatcattgcc atcagcagtg tattgaaata tggcgttgca caacctgctc ctgcattcaa
    10141 gataagaaag tacaagaacc cttgactatc gtttggaacc aatccgcttg caccgatcgt
    10201 caggcccata atgatgtagg ctataataat aggtttcatt cggtctttcc atgttcctat
    10261 aaaactgatc agtgcaccgc ctaagagcat tccgactgcg tagactactt ccactgctcc
    10321 cgcctgtcca acggaaccac caaagtaatc tagcgtcatc aatggataca gactgattgc
    10381 tggcatgaac aacagcatca caaatgcacc attgatcgtt atgtaccata accctttatt
    10441 atccatcaat tgctgcagcc caaattttgc atctttaaaa atagtaagcg gaacttccaa
    10501 tatttcagga gaaacttttg ggatcttcac aaaaatcaat aagccgacac caaaaactgc
    10561 ccccaataca tctaataaaa taaggtaatt gacaggaacg actgaaaaca aagccgcacc
    10621 tagagctggc gcaatgatga aattcgctga ttggaccatt cctaactgtc cattcgtcct
    10681 tgttatgtga gaagatggaa cgattgtcgg cataatcgac tggatcgttg gcatttggaa
    10741 tgtttgtgcg accgaacgca taaacaaaga cacaaaaact agccaaatcg ggaaggattc
    10801 tgaaatcgtt ccgactactg ctaaaataag cgcgaaaatc gctacaataa tatccgtgac
    10861 aatcaaaaga gcttttttat cccagcgatc cactagcggt cctacaaatg ggcttagtaa
    10921 aaccatggga atcattccta gtaaagtagc aaaactcaaa attgtcgccg aaccggtttc
    10981 tcttgtcaaa taccagatga tcgcatattg gacgaccata ctagtgattc ctgacaaaaa
    11041 ctgtcccgat aaaaagaaca aataatttct cttccaattc gttaaaacga ccgactgttc
    11101 attttccata aacatccctc ttttcttttt cattttgtat agcgtttgca caacaatcat
    11161 tatacgggat tcttttttca aaaaatacct ttttccctaa ataacaatcg tttcaattta
    11221 aatttcttta cttagaaaca ataaatcagc aaaatattaa aaataacaac atgtgttaaa
    11281 tttaaaaata aaaaagacaa ctaatttgcg tcgtccttac aaagttagtc atctaacaat
    11341 aggccgtttc aaaactagtt gtctttttat atccacagat cattcgactt tttcgaataa
    11401 cccacggcta atcatcccat ccatcagaaa ataaaattta tcttggctca ttttctgttt
    11461 tggatctttg aaaatattga ctgctttcaa tccatttgct gtagtgatcg acattttcaa
    11521 tgtttgaata tcttttgata cagtgatttt taatttgaat ccttctttgc tttgcggcgt
    11581 ggcatctaat ggtcggatca attgataatt gcctccgttt gtttctgtaa atccattatc
    11641 gcgcaatgta tatctttttg catcaggatg caaacgatag ctaattggtg cgcccagtac
    11701 cgttgctgtt gtttcaaatg ccatgatttt cacctcgata aaattagttt ctctttatta
    11761 taaaagttta tcctgatttt tactaggttt ttcagttaag ttccctaaaa aatgaaggcg
    11821 ttctctcaga catctgttca tataagaatg acttcactag ttattttttg acagcacgaa
    11881 ctaattgatc aaggaaatac tggatgtcgt cttcggtatt tcccaaacca aagctgatac
    11941 ggatggattc ttttagtatc ggtgtatctt ctccatacat tgcttctaat acgtgtgaag
    12001 gctcgatatt tcctgctgta caagctgaac cagtagaaat agcgacccca ttaagatcaa
    12061 gcttcatcag tagcaaatca tttgcttcgc caattaagcg aagattcaac acatgaggga
    12121 gtttattcgt taaatcacca tttacttgat aagggatttc gtttgcttct aatgtatcaa
    12181 gaatcaattg ctggaatcct ttatatttgt tgttacgtgc ttcacgttct tcaggtgaca
    12241 gcagttcaac tgcttttgcc ataccccaga tccctgctaa attttctgta cctgcacgcc
    12301 gcttttcttc ctgctccccg cctaaaagca atgcagggat atttactccg tctcgtttat
    12361 ataagaaacc gacgcctttt ggcccattga ttttatgagc agagatactt aataaatcga
    12421 ttcctaattc ttctggatgg atcgtttggt ttccgtacgc ttgtaccgca tctgtatgga
    12481 aataagctgg atgatccgct aaaagttcac cgatttcttt gattggcatc aaatttccga
    12541 tttcattgtt cccgtacatc acagagacca agatcgtatc ttctcgtaac gcttttttga
    12601 cctcttcgat cgacagattc cctttttcat ctacaggcag atatgtaacg tcaaacccct
    12661 ttgtctctaa atatttcatg gtattaagaa cagcaggatg ttcgatagct gtagtgatta
    12721 gatgtctgcc ttctttttga cgcgagaacg ctgtttctaa gatagctgta ttatcccctt
    12781 ctgttccccc actgttaaag atgatctcgt gaggttttgc ttgcagactg tctgcaatga
    12841 tttgtcttgc tgtttccaat ttttcatgcg cgtggcgacc aaatccatgt atgcttgaag
    12901 gatttccaaa cgtatcttgc atgattgccg tcatcgcttc aattacttgt ggatgcatgg
    12961 gggtagttgc cgcatgatcc agataaatag ttttcacttc attcatccct tccaaattat
    13021 tgctaggcac attctagcat aaaatagcga acaaaccaaa gaagagctca tttttgtcta
    13081 gaggacgttt catgggcata gaaaaaagga gctgtgacaa aagtcttgtc gcagctcctt
    13141 gaagctgctc acttctgtca caacttcttt ccctcttttt tttgtttatt cgaattcttt
    13201 ttcttccaga cggaataacg gactcatcgg tgtattttca tggatgcgtt tgaccgcttc
    13261 acccattaat tctgaacatg taacgattgt taagttttct ggatgacgat tttcttctgt
    13321 gaaaacagaa tcagtgatac aaatatcttt gattggtgca ttgtctaaaa tacttttcgc
    13381 atgatcggac aataatccat gtgacgcaca ggcatatact tcttttgcgc cgttttcttt
    13441 taatacattt gctgctcgtg caagcgttgc tcctgtgttc aagatatcat ctaccatgat
    13501 acatgtcttt ccttgaacat caccgatcac ataaccaccc tcagttgaag catcatctgc
    13561 atgatctacg atggctaatg tgctgttcaa atattctgaa agactacgtg cacgctgaac
    13621 accgctgttt tttggcgaaa caactacgat gtcatctccc gtcagaccta attgacggta
    13681 ataatgagca aacaacggca tcgtaaataa attatctaca ggaatatcaa agaatccttg
    13741 tacttgaacc gtgtgcagat ctaaagtcag tactcgagtt gcccctgctt cttcgatcaa
    13801 gttagcgatc aatttcgcag tgatcggttc atgaggtttc gccgtgcggt cttgcctagc
    13861 atacccatag taaggcaata cgacattgat cgttttcgca cttgctcgct tcattgcatc
    13921 gatcatgatc agtagttcca tatagtaatc attgacaggt gcattcgttg attgaacgat
    13981 atacacatga tcccctcgaa tactttcttc gatattgatg gaaatctcgc catcactaaa
    14041 ttgcttcacg acgcttttac ctaattctgt tccgaacact ttcgcgattt tttcagctaa
    14101 tggacggttg ccattcaaac taaaaatctt caacgtgtca tcctgatact tattactcat
    14161 gtagtcctcc ccaaaacttc tctattcttc gatagtttaa catatttttc caagattttc
    14221 tgttaatttc agcaaattct taaggaagtg aaactgttcc gttactccaa ctggcaggaa
    14281 tgactttatg ataatcgccc cagttgtcgt gtactttgta ataacgttct cctgtttcag
    14341 tttcaaaata agcgtacgct gtcacccaat gattgccata tgtactgcca agtactttca
    14401 aaataccaat catcacaggt ttgccttcac gtatacgttt tgtagctcgc tgccatgaac
    14461 ctagaagagt tgctcgtgca cgctcatgat tgccattctt ctttaaaaaa ctagaaatcc
    14521 ctgtactaac ctgaaaagga atcgtaggaa gtcctagtgg ttggatctgg ctacgcaatg
    14581 ccttcgtcaa aaccaacgaa tcagccgtat ttttcttgcg aatctcattt ggaagcatcc
    14641 accccttacg atagtcttga taataggcaa ggagaactgc tgccgcgtaa gtaccacaaa
    14701 gatagcctga gggggtagcc catttcttat acctcaaaaa acgttcttct ggcaatccca
    14761 cccagcaatt tgcgagttcc tttcctgacc ttttttcttc gatcaaaacg ggaatatctt
    14821 cttttctgtt gatccatcta gataactcga ttggctcttc attgatcagc ttccattttt
    14881 tacaagaaag accctcttga taagtatatt ttgctggaga atattctacc ggatgtcctg
    14941 attgatcgat aatcacataa ctttgatctt caaaaaccag agctaaaaaa agcaatttac
    15001 tttttcttga ctgcagctgc tgagtgctgg ctaatttttt aattttttta tctactttga
    15061 aatataaagg tttatccact acttgattca gttgggatcg cctccttttt ttcattataa
    15121 gtgaaggatt gaaaaaaaca aatcagtttg attgttcgaa ttttcaattt tttatgttct
    15181 atttgttaat tccttcattt tgtaagataa tgggcacacg aaacaaaaaa ggagcgatcg
    15241 atgaatgaac aaagaacgat tgaaacaaga tttctatgaa gcagtcaatg aagaatggca
    15301 aaaaacagca aaaatccctg cagataaacc agctacaggc ggtttccaag atttagttga
    15361 cgggatcgac caacttttga tgggagaaac agacgatctg cttgcccatc cagaaaaagt
    15421 gtcttcagaa aaaatgaaac atttccttgc atattatcaa ctggcaaacg attatgaccg
    15481 aagaaatgag ttgcatgctc aaccgcttca acctataatc gaaaaagttg aatcattgca
    15541 gtctttcgac gaattgaata agcaatttcc agaatggctg ttggatacat taccattgcc
    15601 atttaatctg gatgtagatg cagatatgaa gaatgcgcaa acgaacgcct tctttgctta
    15661 tccgccttct ttattccttc cagataagac gtattatgca tcagaccatc ccaacggtgc
    15721 acaactaata ggcgttttct ttgatatggc tgttcagtta ttagagcttg ccggcaaaga
    15781 aaaaagtcag gcagaagcaa tcacggaaca agcagtgcaa tttgataaat tgattgctcc
    15841 tcatgtaaaa agtgcagaag aaaacgcaga ttacagcaaa atgtataatc cgcaatcttt
    15901 tgatacattt attaaataca cagataaact ggacttgaaa aaacttgtca cgggtttgat
    15961 tgggacaact ccagataaag tgatcgtaac tgatccagtg tacttcgatc atttgaatga
    16021 catcctgaca gaagctcatt ttccgttatt caaaagctgg atgatcatta aaacagtacg
    16081 ttctcttagt ggttatctat cagaagaatt ccgccaagtc agcggaatct attctcgcgt
    16141 attgtctgga acagacgaag caatgcctca gaaaaaagca gcgtattacc ttgcctctgg
    16201 acaattcgat caagtagttg gcgattacta cggaaaaaaa tattttggcg aagctgctaa
    16261 aaaagacgta gaacaaatgg tgaaaaaaat gatcgccgtt tataaaaaac gtctagaaga
    16321 aaatgactgg cttggggatg caacaaaaga aaaagccgtc ataaaattaa ataacttagg
    16381 tatccaagta ggctaccccg ataagatccc agcattattc gaccagttca aaacagtacc
    16441 tgcagatcag ggtggtactt tgctttcaaa tactcttgtt ttcagcaaac tcacattaaa
    16501 agatcgtttc agcaaatgga acaaaccagt ggatcgttca gaatgggaaa tgagtgctga
    16561 cacggtcaat gcttattatc atccatttcg gaatgtgatc gtctttccgg cagcgatcct
    16621 tcaagcacca ttttacagtc tagaacaatc aagctctgct aattatggtg gaatcggagc
    16681 tgttatcgct catgagattt cccatgcatt tgataataac ggcgctttat ttgacgaata
    16741 tggcaacttg aacaactggt ggacagaaga agatcttaca caatttcaaa acaaagcaca
    16801 agcaatgatc gaagaattcg atggtctgcc ttttgctgac ggaaaagtaa acggaaaatt
    16861 aaccgtttcg gaaaatattg ctgatgcagg cggtctaagc tgtgcattgg aagcagcaaa
    16921 aacggaatca gatctttcta tcgaggaatt ctttgtcaat tgggcaacga tttggcgtac
    16981 aaaagcaaaa aaagaatacc aacaactttt actgcaaatc gatgtccatg cccctgctaa
    17041 actccgagca aacgtgcaac caaaaaacct tgacgaattc tatgaaacat ttggtatcac
    17101 ggaacaagac gctatgtatc tagcgccaga aaaaagagtg cgaatctggt aagaggttgt
    17161 gccagaagtg gtcagcttca agaaataaga aggaatttgg gaaaatcgct tctcaaattt
    17221 ttgtgacatt tcggcttatt tttcgaagaa gttacttctg gaataccatg tattagaggt
    17281 tgtgaaaaag ctgcttagtt tcaagtagta agaaaaattt tgtgaaaatg gctttccaca
    17341 tttttgcata aatttgactt actaccgaga aactggcttt tgaacaccgt ttatcgagag
    17401 gttgtgaaat cagcgtcctg acctgagtat tagagaaaaa aacggaaaaa tagttcctca
    17461 tattttgccg tttgttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag
    17521 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa agcagcaagg
    17581 agaacctcat ctataaaaag atagaaggaa gtgagacatc tattggtctc atttccttct
    17641 atttttactt tcctcagtca attgttttac atcataaaag gagaaattcc acttattttt
    17701 ttcgctgttt catgacataa acaactacac cgattaacaa aattcctcca gctaatgcta
    17761 gcaacgggtt attaaattct cccgtttttg ggagtccttg agtgccattt ttttcacttt
    17821 cttttgctgt cctatccgga tgagaaggtt ttccatttgg ttttccattt ggtgattcat
    17881 ttcctggatt ttctctttct ggtttgtcat ttcccggggg cacatcggca gtttctcgca
    17941 cagtgatcgc tttttgctgg gtaacagatt ctgtatgagc agaccatttt tccccatcaa
    18001 atatttccga acgataagtg acttctagcg tatattctcc ttttgaaggt gctgaaaaaa
    18061 tagcggtatg attagaaaat tgagcacttg actgtgtttc aacgatttgg atcttttctg
    18121 ggaaataacg catatcttca aagacaggcg ttccttgatt tggtgtgaaa gttgacacaa
    18181 aaactgtaaa aggcttcagt tcaatgatat ttccttctgc attctccgcc acaccatttg
    18241 cttcttgtat aataatcgga ttattttgtt catttggttt tgtctgattt ggttcttgta
    18301 cgattgcata ctctggatat tccgaagacg gtgaggtttc tccgtcaaaa atagcaccta
    18361 cagataatgg aagaccatcc actctttcct tttctgtttg acgattcccc ggtttaatct
    18421 gcatcagtcc atgaacatgg atacttccat tttcatagcg cgtaacaggc tgctgacctt
    18481 ctttcgaaca tgtatttaga gactcgatcc aatcttttga tatggtatgt ccaagagtat
    18541 tttctgattg tttctctcgt tcattccaat agtagttcaa cgagtggtag atttgatctt
    18601 cgttttgatt caatgggcgg atctgctctc tcatctcaag attctctgtt cggaaagata
    18661 agataccaga agcatggtag tctgtgtttc ctgctgaaga ggtataaaga gccacattat
    18721 atgaaccgtt attaaaactt gtactgctat tgacgataat atctgttccg ctattggaat
    18781 cgattccttt cgcaagattt tcgaaagaca aactattgat caattcatga gggcccgtta
    18841 aagaagaacc gcccattttg aatccattac cgttaccttc tccctcaatt cctggtaccc
    18901 agccgtttcg ataagcaact gagtttttga tgattacttt tccgatcgaa cctgtttcat
    18961 ttttagcaaa aaggtcccaa ccgtcatctg cattatgata cgcgatacaa ccgtcaaata
    19021 catttccttc tcctattgtc agtttggccg caaatccatc ggcatcttca aatcctggat
    19081 cggcattttc aaaagatgta cagttcttga ctaagttatg cgcgggccat gcttcgaaag
    19141 gatcatttga gctaccgctg atttggattc ctgtatttcc attccgataa gcatgaatct
    19201 cttcgatcaa attatgattt ccagaaatct gtaatccttt ttgcatagct gcactatttg
    19261 taatgtcaaa ccctcgtaaa ccccagtagt gactgaacag tgtcatccca tttccagttc
    19321 ctttaaaatc aagtacagga cgcttgtttt ctggattttt ctctgcaatc aactgaatcg
    19381 ggttttctct tgtcccgtta acgccttttg gaatcgtcaa tcctctttcg aaggaataag
    19441 ttccaggcgc catgacgatt gtctgcccag catatgcgta tctcaatgct tgtgtcagag
    19501 ataatggatt ttgtctgctt ccgtttcctg catctgaccc ttctggtgtt acataaactg
    19561 tcgaatattt aggtttacgg tagtccactg tatgaagaat ctcttttgtt tcataactag
    19621 tcatctctac gtattcttct ggcggatagt ttttatctgg cgtaaaactg aaagtaaatt
    19681 cgttcgttcc ttccatcagt tttgtctgat aatcaaattc ccgattcgca gtgatagcca
    19741 agttttttgc gttcttaagc acctctcctg ttgctttatt tttaattgtc aataatccgt
    19801 cagcatttgc tctaaaggac atagtatgat cttggtttcc tgaatgatta ctagaggtca
    19861 cagtgtaaat cggatcaatc aagcgagtag gtctttcttg cccaggagga tctgtcgcag
    19921 gatctgagta agcaacttcg attccttcta ccttgatcgt catattcctt gctgcagcaa
    19981 atcctgcata aataaccgat tcatctgaag cgtataattt ttcccagtca tacatgatgt
    20041 cagaagccat ttcttctcct gtttcaggat cgtaataata tgcctctaaa ccagtattcg
    20101 tttttttcat ctctacgtcc agtttagtaa tcgagttcgt ggccggaaac agattcccat
    20161 gaccgtttcc gaatatatta taagaacctg tttctttttc caagaaccct gctgaagtct
    20221 ctaaaggtgt tgtttccact gagacactcc caggagctgg cgcagcttgc ggatcttcag
    20281 atgatattcc tgtgattgcc cgtgtcccca atccaagtcg catcgtatat ttgctgccgc
    20341 tcgtgttgac cacttcttgt ctatccgagt cccagacata ttcgatccga ctgcctagta
    20401 tggcaaatga atttgaataa aatgcctgcc caaacaaatg attttttggt actgcgtctc
    20461 ttaccattaa agcaaatcct tcttgggcat tcgtataggt ccaagattct atttcgatcg
    20521 tcgcccgcat cctaaagttg atgtctttgg ggattgcttg ataataatag gtcattccat
    20581 ctgatccgct tgactggaat tttccgccgt tattcgtgga agagagccgc acgccgtctt
    20641 ctaatggttc aatggcatac ctactagcac ttgtgctttc tccgaaaaca acccctttcc
    20701 actcttcatt tgcttcttca gattgctttt ttgtcttttc attttctgat tctttcatca
    20761 taggatcatt tccttcttcc gtatccatat cttcactagt tcttggtatt tcctccattg
    20821 ttgacatccc ttcaatgttc acgtgtgcat tttcttcgtt cacagactgt tgttcgttag
    20881 cagcaacttc aacgacagaa attaaaaccg gggtactgag cataaggaga ttcatcatta
    20941 gcgcactgta ttttatacgt ttttttaaca agtgtattcc tcccgtaaca aattagaaag
    21001 cgtttgctta aagtattacc taatttattt ttactgtcaa taaaaaacaa aattataata
    21061 agtgtatttt ataaaaagaa aaaatataac ttaagttgtt gataataaag agatttatta
    21121 acggaaaaag aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt
    21181 ttgttttatg ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt
    21241 tttataaaat ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa
    21301 cgggatcgaa acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg
    21361 catcgactgc tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg
    21421 tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc
    21481 gtttgtctat ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag
    21541 agccgtacaa ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct
    21601 ccggatgaaa ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt
    21661 gatcaataaa cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc
    21721 cagtagtcag ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg
    21781 cgttcatctt agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga
    21841 tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt
    21901 gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac
    21961 agcggctcct tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat
    22021 tttatctggc gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa
    22081 attcgttgct tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata
    22141 tagaggcatt cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa
    22201 ataagctact ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc
    22261 agctgaacct gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt
    22321 aatagatgct aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa
    22381 tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc
    22441 tctcaaaatt tttacttctt cttccattgc gccgataata ccgattttca tctttttaat
    22501 tccttctttc tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat
    22561 aataagagat ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct
    22621 tgttttcgtg attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt
    22681 ttcttcttgc tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg
    22741 tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg
    22801 atccctctac tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc
    22861 atcacaaatc aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa
    22921 ctccagtact tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc
    22981 atgataaata tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg
    23041 gctcagcgac ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc
    23101 gggattttgt ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc
    23161 taaaggtttt cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat
    23221 cccgacaccg ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg
    23281 aacttcgtct acagcaacat caataatttt tccgctatag atttctttgc gactgattgt
    23341 tttttcttca aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac
    23401 tgcttttttt tcatttttca atttttctca aaaactcctt attagtttac catagataaa
    23461 ccttagattt tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc
    23521 cgtatgctaa aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat
    23581 gacatggagg gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc
    23641 tatttctctt tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg
    23701 tagttttagt tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa
    23761 ttcctgcact gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg
    23821 aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa
    23881 tgaatatgca gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag
    23941 cagcgaaagc tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc
    24001 agtttcttta tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca
    24061 atgaccatga gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca
    24121 tcgaccagct gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag
    24181 atgatatcga cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg
    24241 aaccagaaca aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga
    24301 tgtctggctg tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca
    24361 gattcaacaa ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac
    24421 cagttaacga tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc
    24481 tgaccacttc acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt
    24541 taattgacaa actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg
    24601 atgctaatga tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg
    24661 aattctcaca atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt
    24721 ccttaactga actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag
    24781 aagggaacat cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag
    24841 caaaatatca aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa
    24901 aagacggtct gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact
    24961 actttgatgc actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa
    25021 caacgatcat tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc
    25081 agattgccaa tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc
    25141 gcttagctag acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa
    25201 atcaggcctt ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa
    25261 accaagtcgt gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc
    25321 aagtctctga aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag
    25381 ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca
    25441 ctcaaaatga tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag
    25501 aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac
    25561 tgttggattt aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca
    25621 gtttattaca actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt
    25681 ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat
    25741 aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat
    25801 acaagtcaag atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc
    25861 ttttggtttg ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata
    25921 ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa
    25981 gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc
    26041 gatttctgtc ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc
    26101 aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat
    26161 caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac
    26221 tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac
    26281 agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac
    26341 ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc
    26401 accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac
    26461 gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc
    26521 attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac
    26581 tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa
    26641 tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc
    26701 ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca
    26761 ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac
    26821 tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac
    26881 aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa
    26941 tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc
    27001 ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga
    27061 agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag
    27121 gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt
    27181 ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga
    27241 aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc
    27301 taagccccat aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt
    27361 acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga
    27421 catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga
    27481 gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt
    27541 tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat
    27601 aataccgtat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat
    27661 cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta
    27721 cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc
    27781 gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa
    27841 cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc
    27901 acttactaaa ccaatactac cccatgccaa catagggact aatgctatta aaatttccat
    27961 aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca
    28021 agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg
    28081 ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac
    28141 ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc
    28201 taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg
    28261 ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgtgcttcc ttttctatgc
    28321 tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga
    28381 agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca acaatctcgt
    28441 agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc
    28501 cttttagcct atattcttgg atgaggtagt cccgcgaaaa actgtctagt cttgcataat
    28561 acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact
    28621 tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca
    28681 ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg
    28741 ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt
    28801 tttcttcttc tgttcgtcca ccgctcaaat agttttgcaa aggggaagtt ttttgtatgt
    28861 cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca
    28921 gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg
    28981 gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg
    29041 tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac
    29101 gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt
    29161 tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc
    29221 ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat
    29281 aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact
    29341 tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt
    29401 gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa
    29461 aggttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt
    29521 attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca
    29581 ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct
    29641 ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt
    29701 ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt
    29761 tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg
    29821 aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata
    29881 tgcgatttaa tcgcttttgt cttttcaata cagcttaaat ttattatgtt attcttaaca
    29941 attggtctat gatctgttat ttttccttag ttgtttttgt cttttaaatt tccaataaca
    30001 ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata
    30061 aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg
    30121 ccttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt
    30181 gactagatcc tgcttttctc tgattgctaa tgtatttgtt tttgttggaa ggacaacaat
    30241 tttctcaacg acttcataag cgagcatccg accatacact tcaagataga atcggtcatc
    30301 ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt
    30361 aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc
    30421 tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt
    30481 cggaatatag attgctccga tcatatgttg ttcgtagtat tcttttcctg gatttcggac
    30541 atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt
    30601 atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc
    30661 tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct
    30721 gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc
    30781 cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattacctg ttctcttttt
    30841 cattttcttc accttcttat ttaaaaaaac tgcagcaatc ggttttataa aggttgctgc
    30901 agcttatttt tgttgcttat acttcagact tcattttaaa tttacggtac cagctgtacg
    30961 ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag
    31021 caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta
    31081 cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct
    31141 cacctgttgg aagtacataa
//
LOCUS       DAPBBG010000033        31114 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPBBG010000033 DAPBBG010000000
VERSION     DAPBBG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.033"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(334..696)
                     /locus_tag="RD477_001694"
     CDS             complement(334..696)
                     /locus_tag="RD477_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634054.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="RD477_001695"
     CDS             complement(770..1150)
                     /locus_tag="RD477_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDT7634055.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="RD477_001696"
     CDS             1214..2407
                     /locus_tag="RD477_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7634056.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="RD477_001697"
     CDS             complement(2484..3068)
                     /locus_tag="RD477_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7634057.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="RD477_001698"
     CDS             3269..4267
                     /locus_tag="RD477_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7634058.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="RD477_001699"
     CDS             4379..4888
                     /locus_tag="RD477_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDT7634059.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="RD477_001700"
     CDS             complement(4918..5577)
                     /locus_tag="RD477_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDT7634060.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="RD477_001701"
     CDS             complement(5579..6343)
                     /locus_tag="RD477_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDT7634061.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="RD477_001702"
     CDS             complement(6351..7028)
                     /locus_tag="RD477_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDT7634062.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="RD477_001703"
     CDS             complement(7065..8450)
                     /locus_tag="RD477_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7634063.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="RD477_001704"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RD477_001704"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="RD477_001705"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="RD477_001705"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDT7634064.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="RD477_001706"
     CDS             9389..10246
                     /locus_tag="RD477_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634065.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="RD477_001707"
     CDS             complement(10327..10662)
                     /locus_tag="RD477_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634066.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="RD477_001708"
     CDS             complement(10729..12099)
                     /locus_tag="RD477_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDT7634067.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="RD477_001709"
     CDS             complement(12177..12713)
                     /locus_tag="RD477_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7634068.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13022..14503
                     /locus_tag="RD477_001710"
     CDS             13022..14503
                     /locus_tag="RD477_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634069.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14606..15313)
                     /locus_tag="RD477_001711"
     CDS             complement(14606..15313)
                     /locus_tag="RD477_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDT7634070.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15392..15730)
                     /locus_tag="RD477_001712"
     CDS             complement(15392..15730)
                     /locus_tag="RD477_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDT7634071.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15841..16803)
                     /locus_tag="RD477_001713"
     CDS             complement(15841..16803)
                     /locus_tag="RD477_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDT7634072.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16818..18149)
                     /locus_tag="RD477_001714"
     CDS             complement(16818..18149)
                     /locus_tag="RD477_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7634073.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18157..18837)
                     /locus_tag="RD477_001715"
     CDS             complement(18157..18837)
                     /locus_tag="RD477_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDT7634074.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19056..19535)
                     /locus_tag="RD477_001716"
     CDS             complement(19056..19535)
                     /locus_tag="RD477_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDT7634075.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19662..21341)
                     /locus_tag="RD477_001717"
     CDS             complement(19662..21341)
                     /locus_tag="RD477_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDT7634076.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21889..23391
                     /locus_tag="RD477_001718"
     CDS             21889..23391
                     /locus_tag="RD477_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634077.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23404..24066
                     /locus_tag="RD477_001719"
     CDS             23404..24066
                     /locus_tag="RD477_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634078.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24167..24370)
                     /locus_tag="RD477_001720"
     CDS             complement(24167..24370)
                     /locus_tag="RD477_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDT7634079.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24481..25665)
                     /gene="iadA"
                     /locus_tag="RD477_001721"
     CDS             complement(24481..25665)
                     /gene="iadA"
                     /locus_tag="RD477_001721"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDT7634080.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25822..27561)
                     /gene="yfcC"
                     /locus_tag="RD477_001722"
     CDS             complement(25822..27561)
                     /gene="yfcC"
                     /locus_tag="RD477_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDT7634081.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28334..28948)
                     /locus_tag="RD477_001723"
     CDS             complement(28334..28948)
                     /locus_tag="RD477_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7634082.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29554..29931)
                     /locus_tag="RD477_001724"
     CDS             complement(29554..29931)
                     /locus_tag="RD477_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634083.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30231..31112)
                     /gene="galU"
                     /locus_tag="RD477_001725"
     CDS             complement(30231..31112)
                     /gene="galU"
                     /locus_tag="RD477_001725"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDT7634084.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         9285 a         6465 c         5190 g        10174 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgatg aatttattta
    13201 ttctaatcat aaaggagaga tccaaaccaa agaaaccgat caaactaaaa agttagctgt
    13261 tttttctaag ttaaaattat gctacctaaa agaatccaat cgttttaact atttgatgtt
    13321 actaatcaat tatccacaaa ctgctgtacc aaaaaaatat ttacttgaac aactttatat
    13381 atcgccctcc tatctcgaaa aactgacaaa acagctcaat cattccttaa aaaaattcca
    13441 tttacggatc ctatctgtaa aaaattgctg tactcttgaa ggaaatgagt tatctatccg
    13501 tctctatctt tacttttttc tttcagatac atttggaggt aacgattggc cattttcctc
    13561 acttgaatta aatgcactaa aaatgaacag tacagatgag caaatagaaa aaatttcaaa
    13621 acaagaaaaa aatatctact tgctgttaac tttattcata ttacgaaaca aacaacaggc
    13681 atattttcca aaaacttctt ctgaaatcac tgaattgatg gacttattgc agcatacaca
    13741 tgattattca gaatacttta cttctttttc agtgagtcgc ccttgtgaag agacagaaac
    13801 agagagagcc tactttaatt attttttcta ttgttttttt ccaaatcaaa tctcttttcg
    13861 acaaaaacag gagattggtt ttttattttc cacaagcaaa aaccttattt gtcgacaagt
    13921 cggtcaattg atgacttact taactcaaca gtttcctctc aaccatttag aaaataaaat
    13981 accaatttat ttttattttc ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc
    14041 cagcagtagt ttttctacac tttgctttcc agaagccacg tccaatcatt ctagttcttt
    14101 tatacaggaa aagaaattaa gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag
    14161 aaaccaaatc agtcaatacc ttagtcaatt gatccaacac ttactacaaa cagaaacgaa
    14221 agagcagtta ctcatccatt tgcagataac caaacagttt acaggaaatt atcatcttca
    14281 aagccatttg cagcagttat ttcgcaaaga tgctgttaaa attaccgcat attcagaaca
    14341 tgctgatttg atcattacag atcagcatga gaaaatttca gatgaaacgg agactttcta
    14401 tctggataca acgcacaata cagtgttatt tggcaagtta ataaaagcgg ttcatgaact
    14461 gtatatgaaa aaaggttatg agaacaaaac aaagaggatg tgacaaaatt cttgtcacat
    14521 cctcttattc caaataaatg gtattcaatc agctaactct cggtattagc tcagatcaaa
    14581 gcgctgattt cacaacttct ttattttaat ctcttcggtt gccgccgaac aataagatca
    14641 agcgcaacag ctgcaggaat gtcgaaagcg ctgctgcaac ataagtcagt gctgctgcaa
    14701 ataatacgtg tcttgccatc ggtacttctt cttccgtcag tagtccgcct tcactcaaaa
    14761 tttggattgc tcgccgagac gcgttaaatt cgactggtaa tgtcaccaat tggaaaagta
    14821 atgctaaaga aaaagcgaaa atcccgatat tgattaacgt agagttccag ctaagcaata
    14881 cacctaccat aatcaagggg aacgaaatca tcgaaccaaa gttagctgct ggaacaagtg
    14941 ctgcacgaat cttcaatggg acataacccg aagcatcttg taccgcatgt ccgcattcat
    15001 gagctgctac acctatcgca gcaactgaag tagactgtgc agttgcttga gacaaactca
    15061 acatcttatt acctgaattg tagttatctg tgagatctcc ggcaatttgt tgaacaccga
    15121 cattatttat ctgttgactt tgtaatatat attgtgctgc ttgtgtacca gtcacattgt
    15181 ttttgcttct cacttgatcg tattttcgga aagtactatt gacgtacgct gacgcaattc
    15241 cggaaataac taatccaaga atgaccaaaa gaaatgtcgg atctaaaaaa ccatacattc
    15301 cataaccata cattttcgtt tcactccttt tcgtttactt tattttatca ttagtagtag
    15361 gaaaagaatg tgaaaagacc ttgaatattc tttaattctt tttcacaaat tccgatttca
    15421 gtttcatcgg tccaaatcct tctaccttgc aatcgatatt atgatcgcct tctactaaac
    15481 ggatgttctt gacttttgtt ccttgtttaa tggcacctga cgcacctttt actttcaagt
    15541 ctttaatcac tgtcacgcta tcgccctcta acagacggtt tccatttgca tctaatacca
    15601 ctaatccgtc ttctatgaca gtttcttgtt cttctttgtt ccattcgttc ccgcattctg
    15661 ggcaaatcaa caagcctcga tcttcatatg tgtactctga gccacattct gggcaatttg
    15721 gtaatgtcat cttctttctc ctttaaaatc tttagtgagc actattgtaa cggaaaaaga
    15781 agatcttgaa aagatacatt tgtgattttt actacaaaag gcacgttttc gaacgaagta
    15841 tcattctgtt tttctgctct tatacgattg gcatatatct tggatctctt tatagatttc
    15901 ctctacttct gctaaatctt ttgcattcgc accacttgct aatggatggc ctcctccatg
    15961 atggcgttta gccaattcat tgatgatcgg ccccttagaa cgtaaacgta cgcggtaata
    16021 ccctgaaggc tgctgaacaa aaatccccca agccagcact tcgtcaatca ctccaggaag
    16081 tgaaacgatc gaagctgtct ctgaatcatc gattccatat ttatcaagaa tttcttgcgg
    16141 aaggattatt cttcctgcac cattttcatc cacctggata ttttgataaa gataaccgga
    16201 aagtttagcg actttcatcg gcatttgttc aatctctctg ttcaaagcac ttgcatcaaa
    16261 tggataattt ctcaatatcg aagcaatctc taatgtacgg gacgtcgtgg agggatacaa
    16321 aaagcgccct gtatctccga cgattcctgc ataaagtaac cgtgcagcct taacgttcat
    16381 ctttaaagat tcaggaaaca tcaaagcaaa ttctgcaatc atctcactac agctacttgc
    16441 ttttgtattt acccacacaa gatctccata agggtcatca ttgggatgat ggtcgatttt
    16501 gatcagtttt tctgccaact gatatctttg atcactgatt ctgggtgaat tagctgtatc
    16561 agtcacgatg actagcgctc cttggtagaa gacatcttca acttcttgca tttctgctaa
    16621 aaagcgaagc ccttcaatcg tttcacctac ttgacgtatt tctttttctg ggaaactttc
    16681 ccgtaatagt tcggctaatc ctacttgtga tcctaatgca tcaggatcag ggcgcaaatg
    16741 cctgtggata atgattttat cgtatttttt tattgtatct actatttctt gctgtactgt
    16801 catttcactc ctgctttcta agaacgttcc attacttgac aaacaacgat tgctttggaa
    16861 acgatggtgt tttcaataaa cacttcgata tccagtttag cggaacgtct gccgatttcc
    16921 aatacacgtg gacgaatgtc taattcgctt tccaattgga tcagccgcaa ataatgtagg
    16981 ttcacttgtt caatcaagat atttcgtttt tggttcatca ccattgtttt ttgtgcgaca
    17041 tcagaaatga tttcgctaag tacgccaaaa gaaatggttc caacgccatt gaccatttgt
    17101 ggtgctacta cgaatttgaa cttagggttt tccaaacgat ttccttcacg gtcagtatca
    17161 atcgtatgga tatttccaga tatctgatcc gaaatcgtat ctgcaatctg cggttgtcgc
    17221 tggacaagct gcatcgcttt cattacatct tgccgggtaa tcaagccttc caatgtcaag
    17281 tcatcggaaa cgaccggcat gacttctagc ccatcccaga tcatttgatg gctaacagag
    17341 gcaatactca ttgttttctt tacactgatt ggttctttcg tcatcacccg atcgatgatc
    17401 tggtttggcg atttccctaa aacatctttt gccgtaacaa tccccatcaa acgatgatgg
    17461 cggttgacga ctggaaaacg cgaatgctgc gtttcttctg acaaacgctg ataatcagaa
    17521 atcgtatctg tctgctgcaa ataatgagtc ttttcagctg gcatatagat atcactgaca
    17581 agcatgatat cttttttgat caattggtcg cttaacgctc ggttgatcat ggttgcgact
    17641 gtaaatgtat cataagaggt gcgtaagatc ggcatttcaa gatcatcagc tagctttgca
    17701 atctcagcag tcgtattgaa tcctcctgtg atcaatactg cggcgccatc ttccaatgct
    17761 aatttttgga cttccgcccg gttgccgacg atcattagtg atcctggcgt aatatagcgt
    17821 tccatggcat gtttagtcat cgcgccgatg acgaatttat tcaagacctt gtctaatcct
    17881 gccgccccgc ctaaaacgtc cccttcaatg atcctgacga cctcgccaaa tgtcaaacgt
    17941 tcaatatgtt tttttaattt gcgttcgata cggatggtcc ctactcgttg aattgttgaa
    18001 actagtccga cattttctgc atctttgatt gcacggtacg ctgttccttc actgacattc
    18061 aattcttttg cgatgcttct tacagaaatt cgattgccaa caggtaaact ttcaatatat
    18121 tctaaaatca aatcatgttt tgtcgccatt ttctgcctac acttccagcg tttccgagac
    18181 gctcattatt tttccttcac tatcttccag taactctaca aaagattcgg gatcttgttc
    18241 aatgacaggg aaagtattgt aatggattgg aactactttc ttggcacgaa ttgtttgcgc
    18301 agcctttgct gcatcttctg gccccatcgt atagttatcg ccaatcggca agaaagcgac
    18361 atcaatatcg aattcttctg caagcaatcc tagatcactg aaataagcag tatcccctgc
    18421 atgataaatc gtttttcctt ctgcttgaat aataattccg ctaggctcgc ccatatactg
    18481 aacgataccg tctacttcat agcttgaact atgctgtgca tgtacgaatt ttaccacgcc
    18541 gaatggaaac gcatgttttc caccgatatt cataccgaat gaatctactc ctctgctgtt
    18601 cgcataatca gcaatctcaa caatactgat aattgtggct tgattttctt ttgcaatagg
    18661 aatcatatct cctatgtggt cactatgacc atgagtaatc agcaaccaat ctgtctttac
    18721 ttcttctgca gttaagtctg tcaatggatt tcctgtaata aacgggtcaa tcatcacgcg
    18781 ttggccattt tccatttcaa ttgttaaaca ggaatgtcca tgatacgtaa ttttcatatt
    18841 ttacacctcg cgttttctct gtatcttttt tattaaaaac gatttcatca cttgcacatc
    18901 aatgaaaaaa tatagctgct aaatatattt tcattggatt gatagtacag tatgtacaaa
    18961 gcaattgtaa cagataatac aggatgcacc aagtaccaag cagatataat ctcaagtttg
    19021 aggtctctgc tctgcttagc ttctcttctc gtcccttaac taattaaatc agcgtaaata
    19081 ggatggacga tcttttctaa atcggctact tttatccgaa ggcttctccc gatttcaccg
    19141 cttgacacaa ttacttcttc tttttctttt aaagaactgt ctgctaaaaa acaataatca
    19201 ggatgtgcga gttttattcc aataggggtg tttgctccgt ggggatatcc tgtatgcatt
    19261 aatacaaaat ccattccagg aaaaccaact ttacgatctt tcgtcacttg tctcgttttt
    19321 ttgtaatcta agtgatcgtt caaaggaatc aatgcgatga tcaccccagt tttgtttcct
    19381 ttcactacga gagttttaca tatcaaagct ggatcaatcc ctcttttttg tagttcttct
    19441 gtgaaagatt cttctaccac atgactaaaa tcaagtggtt gatagctgat cttttgttct
    19501 tctaaataac gttcgacttt tgtttgactc atcattgatt cactcccttt attctatcgt
    19561 ttattataaa gcgagggatg ctgtattcaa aaattatttg tttttcgcaa aaaaagagca
    19621 agacgagcac tctatagaga ggttcatctt actctttttg tttatttttt atttcgacgt
    19681 tgagtagctt ccataatgac tggcaagatc actgggcggc gttttgtttg ttcaaataag
    19741 taacggccta attgatctcg gatgtcttgc tttaatttgc tccactcaaa atcattgctt
    19801 tctaaatgtt tctcaacgat tgtttcaatc atattgctgc tttctttcat cagatcacgg
    19861 ctagctttta cgtaaacgaa tccccgtgaa gtaatttgag gttttgaaat gattttcttt
    19921 tctcgccgac tgatagtgac aaccgctacg aaaatcccgt cttctgacaa gatttttcga
    19981 tcacgtaaaa caatgtttcc gatatcacct atgcctaatc catcaatcat tacattttct
    20041 gcacttgtag ttcctgcaac actcatacgc ttgttcttat attccagcac gtctccgcgt
    20101 ccagtaatat agatattttt gtatggcatc cctactgcat gagccaaatc tgcatgagca
    20161 gctaattcac gatactctcc ttgaactggc acaaaatatg ttggtttcat caaattgatc
    20221 attaattgca agtcattagg attggcatgg ccagaaacgc gcatattctc agagatcaac
    20281 ttcactgttc caccagctcg ataaatgata tcttccgttt tcgccacgat ggtttccatc
    20341 gcgatgcttg gcgtagtagt gatataaacc aaatctcctt cttcaatctt gacattacgg
    20401 tgtgtaccgt ttgccatttt ttgcagagac ttgatgggtt cacccattcg gccagtttct
    20461 aaaatgatca gttcttctgg atcatagttt ttcatgtctt tcaacgtaac aagcagatct
    20521 tcacttggta attgaagttt ttctaaacgc atcgctgtgc gaataattcg ttcgaaatct
    20581 tgaccagtca agacaacttt tcgttgtgct ttttctgctg catccaatac ttgttgcact
    20641 cgttgcaaat tacttgctac acaagcgacg atgatacgcc cttcccaata tttgatcgta
    20701 tcaaatactt catctgctat ttgggcttca gaagctacag gctgaggatt ttctgcattt
    20761 gccgattcac ttagtaaggc taagactcct tctttgccga tttccgccaa tcgtgcataa
    20821 tctgtttgat acattgggat agccgtttgg tcaaatttaa aatctcccgt ataaacgata
    20881 ctaccttctt ttgttttcag attgatcccc actgaatctg gtatcgtatg agtggtacgg
    20941 aaaaagctca ctactgtttc accaaagtct atttctgtat gctcatctac cacatggaaa
    21001 tccttaaatc ctttggctgc ttcattacga ctgacattca attttgctaa ctcgatcgtc
    21061 aactctgtcc cgaatactgg tacagaaatt tttgacaaca agtaaggaag tgccccgatc
    21121 gcatctgcat gtccatgtgt taaaaagaca ccagctacac gatcaatgtt ttcttctaaa
    21181 tacgtaaaat cagggattac gacgtcgatc cccaacaact cattttcagg atattttagc
    21241 ccacaatcta aaataaaaat atcctcgccc acttctgcga catacatgtt ttttccattt
    21301 tcacgaacgc cgcctaatgg gataatttta atggtactca caaacttcac ctcttcttat
    21361 gacatccaag tttaaagatg tcgattttct tctttatcta aatatatgag ccattccaat
    21421 gaagcatatg caaaatcact atttctcaac ttatggagaa tcagcaggaa aattaccagc
    21481 acatccgcgt tctgacagtt ggatgggcat tttattttgg atatacttca ttaacaaaat
    21541 ggatcttttg cgaaacgcta tagccagaat acttatataa gtattcagct gttccccatt
    21601 attatacaac aaataaacta aatcgctagt atcagcaatt gttagaacaa ctatgcacgt
    21661 aaaaatccct ctgtagcatc taggatttct tttgctattc ctctttcatt tcttcttcgt
    21721 cggtttgaaa catacttttt tcattttacc acattttttg caataactat agtgactttt
    21781 tatatataca gaacctcatg ttgatggtgt gcaaacagca taagatttaa gctagcttta
    21841 agataggttg tctattatca aatgcatcta ctaggaggga tgcaaaatat gaattttatg
    21901 aaacgtgcat ggaaaagtac aaaagtaaaa tggggacgtt cattgttatt gtttgccgtg
    21961 ttcacagcca ttcttatttt tgtgctagct ggcctcacga ttcgaagcgc tgcagaagaa
    22021 gcagcgaatc aagcacaaaa agaagttgga gcaacagtga ctttaagtgc caatcgagaa
    22081 gcttcattta aaaaacagga acaaacttct tcaagcggtt ctaccactga agaacgtcca
    22141 gatccaggca gtttttctct tacaccagtt tccttaacgg acgcggaaaa aatagctgaa
    22201 ttagataatg taaagagtta ttcctttgaa agttccgcct cagctatggc aaaagatgga
    22261 ataactgcca tcactagttc tgattcaagt gaagacagtt cagaaactac tgatgcaggc
    22321 ggtgaacctg ctggaggaat gggcgggaat ccgcaaatga ctcaagcaga ctttcaaatc
    22381 agtggtgtgt cagcaactgc acaaaatagt aatttttctg atggaacagc aaaaatcacg
    22441 gatggtgaag gaatagatga cagcgacaaa ggcacaaaca atgtagtcat tgattctact
    22501 ctagcagaaa caaatgacct ggcagtcgga gatactttca caatcacgag tactgaagat
    22561 gaagatacca cttatgaaat gacgatcaaa ggaatttatg aatccagcga aacaagcggc
    22621 agtatgggta tgaaattcaa cttcatgaat ccgtcgaata cactttacac ttcttacact
    22681 tttgcaaatg aactaaatgg gacatctgac gataacacga tagattctgc tatttacact
    22741 ctatctgatc cagataaaat ggatgagttt ctcaaagaag ctgagaagct gatcgacaca
    22801 gatacattta gcttacaatc aaatgattcc atgtatcagt caatgttgga acctttaaac
    22861 aacgtggcaa gtttttctaa aaatgttgtt ttactagtgg ctgtagccgg tattatcata
    22921 cttactctga tcattatgat taccattcgg gagcgtcggc atgagttagg cgtcttgcta
    22981 tctttaggag aatccagaag taaaatcatt ctccaattgt ttaccgaagt ggctatttgt
    23041 atgattcttg cattaggagt tgcttcgctt agcggaaacg tcgtcgcgaa tgccgtggga
    23101 cagcagctac ttgaccaaca gacagaaacc acgacacaaa accagcaagc tcctggcggt
    23161 ggacaaatgc caggtaacag acaagaacaa ggcggtcaac gcgatggtca acaaggaacc
    23221 acgaataatc catttgctgt ttctgatcaa gtcagcgaat tggaaatcaa tgtcaaacct
    23281 gcacaattgg gactattagc cggagtagca tttggaatca gtctgttttc tgtcttttta
    23341 gcttctatag ggatattaag attaaaccca agaaaaatcc tattaaacta aaggaggaat
    23401 ttgatgttac ggacagaaca attgggattt tggtatcaag atccgcaaga tgcgctttat
    23461 aaagatgtag aactgaattt cgaagctgga aaaatgtacg ctattttagg agcaagtggt
    23521 tctggaaaaa cgacttttct ttcgcttatt tctggactag atgcaccaaa agaaggaaag
    23581 gtatactatc aagatcgttc actgaacaaa atcgggttaa gaagttatcg taaaaatgat
    23641 gtctcgatta ttttccaagc ttacaacttg cttccttatt tatcggctct agacaacgtc
    23701 atagcagcaa tggaaatcac taagaccaag caagagaatt atcgacaagt tgctttagat
    23761 cgactggctc gtgtaggaat tgatgaaata ttagcaaaaa aacaggtgac caaactttcg
    23821 ggaggtcagc agcagcgagt cgctatcgtt cgtgctatct gctgtgaaca tcaactaatc
    23881 gtagcggatg agcctactgg aaatctagat gaaacaactt cacaagatat cgtcaaactt
    23941 ttccaagaaa tcgcccatga acaaaatcgc tgtattatca tcgtgactca tgagattgaa
    24001 gtagcaaaaa gctgtgatga agtctttgaa ctaaaaaata aaaaatttca aaaaatcgaa
    24061 gtttaaaaaa caggataatg atcaaataaa aaatccgaac catatctgtc tcgcagatat
    24121 ggttcggatt ttttatttgc ttgattcaat tagtaataag tggtttttat tttttttcgt
    24181 cttttcgaga aaacttgtct ttgacttcgt cgcctaaatt tcgagctttt tctttgatgt
    24241 cagcacctag tgattgtgct ttcccttctg tttctttgct cttgtcgtct gttgctttgc
    24301 cgtatgcttc ttttccttca ccgactactt tatctttcat atctttagaa cgtcctttta
    24361 aatctgccat tgtgcttcct cctcttagtt tgtttattga tctaccttca gggtaacatc
    24421 aacaacagat acagacaaac agaacgctcg ttttctaaaa aagatagtta gagctgttga
    24481 ttaaaaactt ttttcgaaag tcccccatac tttgacttct ttttcttgga ccattatttc
    24541 tcctttagcg ataactgtat caatatcaag cgtttcttta tccaagatac acagatctgc
    24601 atcagcacca atttcgattt ttcctttttt atccaatttc aatatacgtg ctggattact
    24661 ggttatagct cgtaaggcga tttccaaagg gatactttct ttttgcacag cttctttgat
    24721 ccctactagc aatgccttgg cacttcctac tcctaaccct aggaaatgat tgttttcatc
    24781 aaaataaggc aagcttcctt gtccatctga ggtcatcgtg aaattatcta aagaaacatc
    24841 ttcctcgatc aatcgtttca gtgctttgct gaagcgaact tcaccatctg tcttctccca
    24901 aaaatcagga tcttcgctag cagtgaagtc aatcgtgcca ccacgtttgg caaatgccac
    24961 gcaagcttca aataattctg gtgtgcgatt tgcatgagta ggcaaaaatt gtgtgatcgg
    25021 aatttctgtt tcttctacga ttcgtgtcaa tagctctagc tttctttttc ctccgcctaa
    25081 gtggacattg atgatcccag cttttccgga taacatgcct cctacacgag catctgctac
    25141 tgcacgagcg aactcttcaa aacttggctg agaagaacgg tgatcggata ctgcgatttc
    25201 tccgattccg ataattttat caattgtcaa aaagtctttg atgatagaat ctgttactgt
    25261 ttttaccggc aatcggtacg aaccttcgta tacataagtg gagattcctt ctgcttccag
    25321 acctttcgct ttgctgatca acgcagtcat atctctacct tctccgtctg ttcccaaaca
    25381 accgacaact gtcgttacac cagctgtagt caattggctc aatgtgactt ctggcgttcg
    25441 attttgataa ccgccttctc ctcctccacc taaaatatgg aaatgacagt caatgaaacc
    25501 cggggttagt acttttccct cacctgaaag ttcttctact tccactcctt cgtagccgcc
    25561 ttctagctgt tcttctacag ccaaaatctt cccaccggca atcagtacat ccatttttcc
    25621 taagtaatct ggtgcaaaca cttcaatttg tcgaatgatt ttcatacatt tttcctcttt
    25681 ctttaagtat aactgatgat aggatcttaa aaaataatcc aaacgatagg atctttactc
    25741 ctttgatttt accgtaaaat gacggttctt ggagtcctta tctactgttt ggattacttt
    25801 tttatttaca ggtctccagt actactgcag acctattgca atggcaatca tgatgaaaat
    25861 agttcctaaa acaaacagga atccttgcat cttgatttgg aatcttgccc attttgccca
    25921 ttcgatccgt gcaacaccta gcacaccaat caagctggct gatacgggtg taaaagcgtc
    25981 cataaaacct gaaccaagct gataagcaag aactgctact tgtctggata cacctactaa
    26041 atctgccagt ggagccatga ttggcatagt caaagctgct tgtcctgaat ttgatgtaac
    26101 aaccaagttg aacaaacttt ggaagatgta catcgcccaa gcaccaatga cagctggaac
    26161 accggtcaac gccgtcccga tcccatgcaa aatcgtattc aaagctgaag gaacagatgc
    26221 atcagatcct cccaagacaa gaagaatccc tttggccata cctactacga ttgctgtacc
    26281 agctaaatca gctgcaccag attggaaaga agaagcgatg tcattgactt tcattccatc
    26341 taatttgaaa agaacagcaa tgattccagc aactaatccc atcacaaaga attgagaagc
    26401 gatttctgga atgtaatagc ctttttgagt cacgccccaa acgatccaga caaggacaac
    26461 taacatctct aataagatca atttatgccc aagcatgaat tttttatttt catcagatgt
    26521 ctttttgata tgagaacgga aataatcatc tgatttgtaa gtcaacgaac tttctggttt
    26581 tttacggatt ttttctgcat atatcatcat ataaccagcg gctaatgcag taacaactac
    26641 ccacataatc aatcggaatg tcgcaccaga caacacgggg attccagcaa tcccttgagc
    26701 caccgctacg ctaaacgggc tcatccaaga ggtcgcattt ccgacttgag atgcgacata
    26761 agtcacggtt actgctacga tcgagtcata tcctaacgca atgacaaatg gaaccatcac
    26821 cattgagaat ggaattacct cttctgccat cccgaatgta gcgccaccga atgaaaaagc
    26881 aaagaataat agcggtaaag ctaaacgctc cagtccctta gttttactga taaacgcata
    26941 gatacctgcg tcgatcgctc cggtacgcat aataatacca aaagcacctc caacaacaag
    27001 gatcaatgca gcgatcccta cagcagatcc atacttatct ccagatacta gtccttcaaa
    27061 aacaaaattt aagaaaccaa agccgccaaa atcatctgtt ccccatattt tggctgtttt
    27121 atgcaatttt ttagaagtat catagatatt ttcgccgtac tgatcgtaaa gcacatcatc
    27181 tgtcaaggag atttcatcta gtttttcctg cgtaagattt ttttctccgt cagcaatgac
    27241 tttttccaat cctttttccg gaacatccag tttttcacgt tctgctggat gatcttgcag
    27301 ttcttctaat tgatcaaaca catacgactt atccagctcg taagcataac ggaaggaatc
    27361 ttgacgtaaa acagtacgag tacttgtttc gccatttgca tctttgtatt caatatcttg
    27421 cgtgctgaac tttcctgctg gaacgacaaa agtcagtatc catgcaaaga tgactacacc
    27481 aaaaataatg acatacgtat ggggtgtcga taatttagtg aagtcgcgtt tcggttttcc
    27541 tgaagaattt gttttcttca aatttatttc ttcctttctt ttttcagtaa ttttgatgaa
    27601 taaaaaatgg ataatcaaat gaattataca gtaaaaatga catttccaca tgaataattt
    27661 attagaaaat cacttttttc tattttgttt tttttctcct atgtatgatt atttttttct
    27721 ctttccattt atattaagta ttttatttct ctgtttttcc ttaaaattct ttttttattt
    27781 ttagattaga gaaaatctcg tcctgttttt ttcattttac aatcattgaa aaattgaatt
    27841 tcattgcaaa aaaaataata aacacttata aatatccatt atctatcctt caatttttgt
    27901 gtattttatt tctattagaa aaaggtatcc tgttggacaa ttttgttata tcaggtaaac
    27961 ttcattgcaa taatgataac aaatgtacta taattacttt gtaaagagtt ttggggaaga
    28021 ctctttcatc aacgtgtatt aagagacaag aaagaatcca tatggattct ttcttgtctc
    28081 tttttccact actttttatt tttctgacac tacatattct tataactcat ttctcataac
    28141 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
    28201 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
    28261 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
    28321 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
    28381 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
    28441 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
    28501 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
    28561 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
    28621 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
    28681 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
    28741 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
    28801 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
    28861 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
    28921 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
    28981 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
    29041 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
    29101 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
    29161 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
    29221 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
    29281 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
    29341 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
    29401 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
    29461 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
    29521 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
    29581 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
    29641 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
    29701 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
    29761 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
    29821 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
    29881 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
    29941 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
    30001 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
    30061 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
    30121 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
    30181 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
    30241 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
    30301 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
    30361 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
    30421 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
    30481 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
    30541 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
    30601 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
    30661 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
    30721 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
    30781 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
    30841 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
    30901 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
    30961 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
    31021 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
    31081 gcggcaggaa tgactgcttt tttgaccttc actt
//
LOCUS       DAPBBG010000034        29056 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPBBG010000034 DAPBBG010000000
VERSION     DAPBBG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.034"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..225
                     /locus_tag="RD477_001726"
     CDS             <1..225
                     /locus_tag="RD477_001726"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDT7634085.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            207..512
                     /gene="kduI"
                     /locus_tag="RD477_001727"
                     /pseudo
     CDS             207..512
                     /gene="kduI"
                     /locus_tag="RD477_001727"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            532..1335
                     /locus_tag="RD477_001728"
     CDS             532..1335
                     /locus_tag="RD477_001728"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDT7634086.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1584..2648)
                     /locus_tag="RD477_001729"
     CDS             complement(1584..2648)
                     /locus_tag="RD477_001729"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDT7634087.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2645..3730)
                     /locus_tag="RD477_001730"
     CDS             complement(2645..3730)
                     /locus_tag="RD477_001730"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDT7634088.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="RD477_001731"
     CDS             complement(3743..4720)
                     /gene="mvaD"
                     /locus_tag="RD477_001731"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDT7634089.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="RD477_001732"
     CDS             complement(4713..5657)
                     /gene="mvk"
                     /locus_tag="RD477_001732"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDT7634090.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5973..6632
                     /locus_tag="RD477_001733"
     CDS             5973..6632
                     /locus_tag="RD477_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7634091.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6717..7622
                     /locus_tag="RD477_001734"
     CDS             6717..7622
                     /locus_tag="RD477_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7634092.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7624..8256)
                     /locus_tag="RD477_001735"
     CDS             complement(7624..8256)
                     /locus_tag="RD477_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDT7634093.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8407..8532
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8576..9100
                     /gene="infC"
                     /locus_tag="RD477_001736"
     CDS             8576..9100
                     /gene="infC"
                     /locus_tag="RD477_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDT7634094.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9172..9372
                     /gene="rpmI"
                     /locus_tag="RD477_001737"
     CDS             9172..9372
                     /gene="rpmI"
                     /locus_tag="RD477_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDT7634095.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9425..9784
                     /gene="rplT"
                     /locus_tag="RD477_001738"
     CDS             9425..9784
                     /gene="rplT"
                     /locus_tag="RD477_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDT7634096.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10136..11374
                     /locus_tag="RD477_001739"
     CDS             10136..11374
                     /locus_tag="RD477_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDT7634097.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11422..13551
                     /locus_tag="RD477_001740"
     CDS             11422..13551
                     /locus_tag="RD477_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDT7634098.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13526..14215
                     /locus_tag="RD477_001741"
     CDS             13526..14215
                     /locus_tag="RD477_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634099.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14623..15309)
                     /locus_tag="RD477_001742"
     CDS             complement(14623..15309)
                     /locus_tag="RD477_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7634100.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15445..15639)
                     /locus_tag="RD477_001743"
     CDS             complement(15445..15639)
                     /locus_tag="RD477_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634101.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15689..16129)
                     /locus_tag="RD477_001744"
     CDS             complement(15689..16129)
                     /locus_tag="RD477_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDT7634102.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16133..16978)
                     /locus_tag="RD477_001745"
     CDS             complement(16133..16978)
                     /locus_tag="RD477_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDT7634103.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17127..18545
                     /gene="pepV"
                     /locus_tag="RD477_001746"
     CDS             17127..18545
                     /gene="pepV"
                     /locus_tag="RD477_001746"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDT7634104.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18621..19823)
                     /locus_tag="RD477_001747"
     CDS             complement(18621..19823)
                     /locus_tag="RD477_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634105.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19810..21597)
                     /locus_tag="RD477_001748"
     CDS             complement(19810..21597)
                     /locus_tag="RD477_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634106.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21610..22563)
                     /locus_tag="RD477_001749"
     CDS             complement(21610..22563)
                     /locus_tag="RD477_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7634107.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22748..23098)
                     /locus_tag="RD477_001750"
     CDS             complement(22748..23098)
                     /locus_tag="RD477_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDT7634108.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23298..24377
                     /gene="pepA"
                     /locus_tag="RD477_001751"
     CDS             23298..24377
                     /gene="pepA"
                     /locus_tag="RD477_001751"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDT7634109.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24520..24840
                     /locus_tag="RD477_001752"
     CDS             24520..24840
                     /locus_tag="RD477_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDT7634110.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24862..25326
                     /locus_tag="RD477_001753"
     CDS             24862..25326
                     /locus_tag="RD477_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7634111.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25522..26127
                     /locus_tag="RD477_001754"
     CDS             25522..26127
                     /locus_tag="RD477_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDT7634112.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26166..27455)
                     /locus_tag="RD477_001755"
     CDS             complement(26166..27455)
                     /locus_tag="RD477_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDT7634113.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27883..28362
                     /gene="rlmH"
                     /locus_tag="RD477_001756"
     CDS             27883..28362
                     /gene="rlmH"
                     /locus_tag="RD477_001756"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDT7634114.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28565..28843
                     /locus_tag="RD477_001757"
                     /pseudo
     CDS             28565..28843
                     /locus_tag="RD477_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(28862..>29056)
                     /locus_tag="RD477_001758"
     CDS             complement(28862..>29056)
                     /locus_tag="RD477_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7634115.1"
                     /translation="LLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYS
                     VLLSRAQRRFEGTLKYSLGY"
BASE COUNT         9194 a         5415 c         5888 g         8559 t
ORIGIN      
        1 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
       61 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
      121 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
      181 gcttcagcga tagctgtcat ttataaatgg gttatacgat tttagaacca ggaagtgctt
      241 ggaatacgat gccttgtcat acacatgaac gtcgtatgga agcttatgtc tattttgatt
      301 atgccaatga agatacacgt gtgttccata tgatgggcaa accagatgaa acaaaacatt
      361 tagtcgtaga caatgaacaa gccattattt cgccaagctg gtcgatccat tcaggtgtag
      421 gtacaagcaa ttactcattc atctgggcaa tgtgcggaga aaatatcacc tatacagaca
      481 tggatatggt gcaaatggat caattaaaat aagcataagg gagagaaaat gatggaattt
      541 agtatggata tgtttcgttt agatggaaaa gtagcattag tcaccggtgc tgtctatggt
      601 attggatttg agattgcacg ctctttagct acagcaggag caaaaatcgt atttaataac
      661 ttgaatcaag aatcagttga tgaaggtata gcgcattata aagaagcagg catcgatgca
      721 aaaggttatg tctgcgatgt gaccgatgaa gaagcggttc aagcgatggt tcaacagatc
      781 aaagaagatg taggttcagt tgatattcta gtcaacaatg cagggatcat caaacggaca
      841 ccgatgatcg aaatggatgc ggcagacttc cgccaagtaa tcgacgtgga tttaaatgcc
      901 ccattcatcg tttcaaaagc agttattcgg gatatgatcg aaaaaggcgg cggtaaaatc
      961 atcaatatct gctccatgat gagtgaatta ggacgcgaaa cggtcagtgc ttatgctgcg
     1021 gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt ctgaatacgg tcaatacaat
     1081 atccaatgta atggaattgg accaggttat attgctacac cgcaaactgc tccattacgc
     1141 gaaacacaag aaaacggtga acgtcatcca ttcgatcaat ttatcgttgg acgtacgcca
     1201 gcagcacgct ggggcgagcc aatcgatttg gctggaccat ctgtcttctt ggcttctagc
     1261 gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg gaggcatact tgcctatatc
     1321 ggaaaacaac cttaaaagag ctttagtaca ccgtttagtg gagaggttgt gaaatcagcg
     1381 gcttgaacga ataccgaagg acagctgatt aaacatcatt tattcgaaaa aagaggctgg
     1441 gacagaagcg tttaactcca agaaataaga aggaattcac gaaaattgct tttcaaattt
     1501 ttgtgaattt cagcttattt ccgaaggagt tgcttttgct tccgccgttt atacgtgttt
     1561 agagcgtggg ataaaagtgt tctttacttt tgtcccacgc tcttttctgt gcgtcttcca
     1621 tacttcacta agtcgatccc tcttgcttcg caccaatcct tcactggtcc ggaaaagatc
     1681 agtgattgtt ggtgtagggc cgcggtattc gttgcgccga ccatagtata aagcagacga
     1741 agctcttctt gccattgctt catcagtata atagtttctt ctacgccgtg attcatcaga
     1801 tgcgtcagta cggttcctga agtacccact gcatttgcgc cgagacaaag ggctttaaat
     1861 atgtcatagg cattgcggat acctccggag gctaggattt ccatagaagt gtctgcttca
     1921 tttgcttcaa gcaatgaaga gactgtcgat tgcccccagt cagctaaata actcagttcc
     1981 cgcttggagc gacgggcgtt ttcgatctgg gtgaagcttg ttccgcttcg accgctgata
     2041 tcaactgtat gaactcctaa agcagctaga tcattgagcg tttctcttgt catgccaaaa
     2101 ccgacttctt ttacgatgag ggggacgtca atggctgttt gagtctcttg aataagtact
     2161 ttccaattgg taaaatcacg gtctccctca ggcataacca actcttgagg tgcattcaaa
     2221 tgaatctgta aggcatctgc gtgaaacaat cggatagctt cttgggctct ttcgacagaa
     2281 gtccctgcac cgatgttggc aatgattttc ccgtgaggat attcttgtcg cataatcgta
     2341 taagtatcgg ctaaagaagg gtctttcaag gcagcgctga ctgaaccagt ggcaatcatc
     2401 aaatctgctt ctcttgcgac gattgcaaga tcttggttga ttttttttgt tttttcgctt
     2461 ccgccagtca tcgcattgat ataaaatggg ctgcttagtg taaacccaac tgcctgtgta
     2521 cttagatcga catcagaaac tgctatttgc ggcaaagggt tatggatgat ccgtacgaag
     2581 tcaaattcgt ttttttgttt atcatgaaag gctttagcta gtgaaacatg ttcgtctttt
     2641 cgattcatcg gttttccttt ctttgatcgc tatagacatg taacggcagt ggagtgattt
     2701 ctgctttttc ccatgcactc attaaaggaa ggatgccaga tttctggtca acaattacga
     2761 ttccgcaatc gccaccacct gcaccagaag attttgcggc tccttcatac tgttcagcta
     2821 aattacacaa tttgttcaaa gcaggcgttt cgatgacgac cccagtgatt gcagataaat
     2881 catgcagtaa ttgtcggttt tttcgaatca tctgttggat caacgttaca ttattttctt
     2941 taaacccttt gatcatttcg ttgacacatt ctgtactgtt ttttaagaaa agctgataag
     3001 ccaccatttt gtcttctctc gaacggtgaa cttgatcgac caaatcagaa gtagaggcag
     3061 ggctacccgt ccaaccaatc aataaacgta aatcttcagg agcaatcaat ggctcaatgg
     3121 atagacctgg ccaatccagg gcaagtaact cactgataga atgctgagtt tcttgttctt
     3181 ggagccaagg atggtcgaag gttgagaaag cgatccagcc accataacag ctagcagcga
     3241 tgtcgccgca ggaaccatta tcttgaactg ctaaattggc tagtgctgca atcttgaaaa
     3301 tctccaattg agataaattc aacgcgtaaa aaacattcaa ggctttgacc gttgcaacgg
     3361 tcacggcacc gctagaaccc aatccatatt tccggccatt tgaactgtct aactcacttg
     3421 ttactttcag atcataaaag gataaaagaa tgtttttttc ttgtgcatac ttttcagtca
     3481 aacgaatagc agcaaggata taatgaaaag gattttcccg aatatctaaa actaattctc
     3541 cgttgcgtct tgtccaacgt acaggcatcc cactatattg tgcagattgg atacttccga
     3601 cttttcgtgc ggattctaca gtgactgtta cgaattgatc gactgcagcg ataactgctg
     3661 gatgtcctgt ttcaacaact gcatattctc cggcaatata gagtttgcct ggtgcagata
     3721 cttcaatcat aaaaatctcc ctttattcaa taatcgcaat tcctggacca gcatgggcag
     3781 taattacttg ttctttgcta aataatttgg taaatgtttc ttgtacctca gataaatggc
     3841 tgttttccac taaaactttg acattagggc cagcatccat cgtaaagtag catggaatgc
     3901 ctttttttct caattgccgc accgcatcca tcgcttttaa gctgtcagga gaccagtaag
     3961 tgaatggagg ctgggcagct aaagtcgttc catgcatttt tagtccgttt ctttccatcg
     4021 tttctccgag aagttggaaa tcttttgttt tgattgcttg tttcaattga tatagatccc
     4081 cttcaacgga atctaaccag ccttgataaa aactggatgt ttcgactgtt cgacgcatcc
     4141 catttctgct ggacacttct tttttctggt cgttgatcaa aacgaaaacc attgctaaat
     4201 cgtcttcgaa agaatcggaa gggactggct tagcgtaaga acttaagtcg tcatggcctt
     4261 tttcccattc aacgaaacca ccgaaaatac ttcggcaagc tgaaccagac ccgcgtcgag
     4321 caaatcttga aagagagaga tcgtctaatc caagcttaag agcagtattg caagctcctg
     4381 ctaaagcagc tagcccgctg gcagaggagg ctaatccagc tgcggtagga acgaaattct
     4441 gactgattac tgaagctttt agtgagaggc cggcttcttt acgaaaaagg tcaagaaact
     4501 gactgacttt ttttgttgct ttttcgtctt gtaaggtgcc atctagataa aattcatcta
     4561 ccatatagct atcagaaaag atgacttctg tctctgtata aaatgcatcc aatgttaacg
     4621 aaaggctgtt gttcattgga aggatgagtt cttcattttt cttaccccaa tatttgatta
     4681 gagcaatgtt ggtatatgcg cgtgctttgc ctttaaacat aggtatgtac tcctaatcct
     4741 tgtatccaag tttcagcagc acccgcatct tcaagtgctt gactgatttc ttgggctttt
     4801 gtttttgttt gtgctaatgc aatcatacag ccaccgcgtc caccgccagt taatttggca
     4861 cccaatgccc cggtatcttt tgctgtttgg atcaatagat taagaaaatc attgctgatt
     4921 gtcagctttt ccagaagtga ctgtgattca tccattgtct gtgccaacgt ttctggtgaa
     4981 ttctcaatga ttgcttgttt tgcttgcttc gtcaagtatc ctaatttttg gatcatttgt
     5041 ccagtttcat gaggctgggt ttcaaagagg tgagcgacat ctttgacggc ttctcttgtt
     5101 tgtcctttga ttcctgtatc agcgacaatc aaaaaagcat cgatgttcaa agaaaagtag
     5161 tcaaaaggat gcccttttgt aaaataaatc ggctgcaagc tgctagtagc ggctgcatcg
     5221 attccactag gattaccgtg ggcgattttt tccgaaagct ggacattttc taatagtatt
     5281 tcacgagaca aaggaaatgc taagtaatca taaaaagcac gagtgactgc tgtggcgact
     5341 gcagcgcttg atcccattcc acgttcagca ggaatcgtgc tttcaatggt cagttgcaag
     5401 tttttttgga tgtcatgctg tttttttaag tgaatgaata attttttgat attttttaat
     5461 gcatgaggag cttcggctaa atttcctgaa tagtaggaag aaaccagttg atcgtccatt
     5521 gcatccagct cttcaaggaa tgcggtgact tttgttgcat agaaaggaaa ggcaatcgct
     5581 ggttctccat aaacaaccgc gtgctcgccc atcaatatga tctttccgct tgattctcct
     5641 tggccatagt ttgccataat ctaactcctt ctcacgtaaa agtgattttt aagcaaaaaa
     5701 taaagcgccc aaaacgagtt gcgctgcttc acgaattttt ttgtccacta agggtctcaa
     5761 aaaacattcc tcctttattt tactaaaacc atctcagaag taaaactaag aatacgtttt
     5821 ctaaagaaaa aacacttttt tcttaagaat tttttgactt tttttcttct ttgactagaa
     5881 aaaatacaat tgacactact agatattggg ggtatattta aaaaaggttg gagttaaaca
     5941 caatatttag tgatgaagtg agctggaggt aaatgagcgt gatcgtttta gcaggtacaa
     6001 ttggtgccgg aaaatcaagt ttaacagaaa tgatggctga acactttgat agtcaggcat
     6061 tttatgaatc gattgatgat aatgaagtct tgcccttatt ttatgcaaac cctgaacagt
     6121 atgctttttt gctgcaaatt tattttttga ataaacgttt tgcaagcatc aaacaagcaa
     6181 tgaaagatga caataatgta ttggaccgtt cgatttatga agattcactg cttttccatc
     6241 taaatgcaga tctaggaaga gcaacagaaa cagaagttcg tgtgtatgat gaactgctgg
     6301 aaaacatgat ggaagaactt ccctatgctg cccataaaaa acatccggat ttattagttc
     6361 atattcgtgt atcttttgat acgatgttag agcgtatcga aaaacgtggt cgttcgtatg
     6421 aacaattgtc ctttgaccca agtctatacg attattacaa agaattgaac agacgttatg
     6481 atcaatggta tgaagaatac aaagaaagtc caaaaatcca aatcgatgga gaccgctata
     6541 attttgtgga agatcctcaa gcaaaagaag aagtcttgaa aatgatcgaa gaaaaattag
     6601 cagagattcg ccaaaccaaa gtatccattt gatttacagt tgctcaaata aagggtaaac
     6661 ctttatttga gcgacttttt tctttatact ggaaacaaat gtcaggagga tagagtatgc
     6721 gggaaaaaca ctattttaat caagaccgta atttttggat caatgtacag caaaacgatg
     6781 tgctagctgt taaaaactta aaagaagaat atggcattag tgacgagatg ctgacttatt
     6841 ctctagataa aaatgaacgg gcgcgcgtag agtacgattc ttttgatgaa gcgttactgc
     6901 ttgtatcaaa tgttccgcac cagcaaaaag tcgataatca ttatgaaact agtcctatcg
     6961 cttttatttt aaaagaagac ggtttgttta catttaccac gccgaatacc gagtacgtga
     7021 ttcgactaat tcgatcgctt ttggaacgga tgccagacat gagtgtatac agtttattgt
     7081 ttcgtacatt gtttttgatt tctgattcct tttttccttt aatcgaggaa gtgaatagtg
     7141 aacgtcagcg gcttaatctg aaattaagag aaaaaacaac gaacaagaat ctgctgcaac
     7201 tttcagattt agagatcggt ttggtttact tagttacagg aacaaaacaa aatgtagtac
     7261 ttttagaaca aatcaaggca ctggctattt atcgcaagtt gtcagaaaag gaaaaagaag
     7321 agttggacga tgcactgatc gaagcacgac aagcagttga aatgacgaac ttagcttcgc
     7381 agattttaga ccagctctct ggcacttata ataatttgct gaataacaac ttgaatgaca
     7441 cgatgaaatt tttgacggtc tggtcgctga tcttgaccgt tccaactatt gtgacaggat
     7501 tcttcggaat gaatctccag ttacccttta cccattccgt tttcggatgg gaaatcgcct
     7561 tgatcatcag tttggtctta tctatttgga tgcttattgc actgtggcgg cggattcgtt
     7621 gatctagtct ttgatctgca aaccaagggc ttgggcgatc acaagtgctt gctcttgatt
     7681 gacgattagt cccgccaaag aatcttgaga taaagcgatt gttgtgaaga aattagtcgt
     7741 aaagtctaat cccttcagag aagttcgaaa ccagttcgaa ccggttaact gattgtcaga
     7801 caaaaaaagg tttttccaag tgacctcata aaattcgctg tcttctaatt gacaatgatc
     7861 gaaggagaca gctttcaagt ttgtactact aaaggaagcc atcgaagcaa tacaatcagt
     7921 gaaagtacag tctcgaagat aactttcggc aaaattcgta cctgttaatt tacattgatg
     7981 aaacacgact tggtggaaac tgccaccaat ccactccaga ttggaaaaat cacatttttc
     8041 aaaaatgaca ttactgcatt caaaatgacg taaacctgtt ttttgcatcg taagtttttg
     8101 gatatgactc tctcttaata ccatattttt ggcatcgaga tagctttggt tacctccgtt
     8161 gatcagaaga ccagagaaga tctcctcatc ttctaaataa aactctccct ttgtaagaag
     8221 aggaagagaa ggtggaatag gttcgatttt tttcatgtgg gttcacctcg tttttcttta
     8281 gtatagcaaa gaagcgggaa tcactgctag aaagcctgct aatctaatca ttttcagaaa
     8341 aggaagacaa actgtatgaa aaacattgac aaaaagatta gtttttgcta tactataatg
     8401 gttgagaaat aaaagcagaa gcacccgctt ctcgccttag ttgataatgt cactgggcta
     8461 gatagttttc actggttcta ttaggtgaag tacgcgcggg ttctgtaaga gaactcgttt
     8521 ttttattgcc gtttattcgg taagcttttc tttctctcat aatatttgga ggtgaatgac
     8581 catagcaaag gatatgatgg ttaacgacgg cattcgtgca cgagagttac gattgatcgg
     8641 ttcagatggt gagcaattag gagttaagac aaaagcagaa gcattgaata ttgcagaaca
     8701 agcaaacttg gatcttgtgt tagttgcccc tggtgcgaaa ccaccagttg cgcgaatcat
     8761 ggactacggt aaataccgtt tcgaacaaca aaagaaagac cgcgaagcgc gtaaaaaaca
     8821 aaaagtgatc aatgtaaaag aagttcgctt aagtcctacg atcgacttga atgacttcaa
     8881 tacaaaactt cgtaatgcac gtaagttctt ggaaaagggc gataaagtga aagcttctat
     8941 ccgtttcaaa ggccgtgcca ttacccataa agaaattggt caaaaagtct tgaatcgctt
     9001 agctgaagaa actgcggata ttgcaacagt ggaacaaaaa gcgaaaatgg acggacgcag
     9061 catgttcttg acgctggcac cgaaaaacga cagtaagtaa tttgaatgac taactggctg
     9121 ttggacagcc gatagtcgga taacgaaaga ttttgaggag gaaatatagt catgccaaaa
     9181 caaaaaacac accgcggatc agcaaaacgt ttcaaacgta ctggtaaagg cggattgaaa
     9241 cgcttccgcg cgtttacaag tcaccgtttc cacggcaaaa caaaaaaaca acgccgtcaa
     9301 ttgcgtaaag caagcatggt ttctagtggc gacttcaaac gtattcgcca acaactagca
     9361 aaaatgaaat aagaagtaca aataacagaa tctgaagatt tatttttatt aggaggaatt
     9421 atacatggca cgtgttaaag gtggaacagt aactcgtaaa cgtcgtaaaa gagtgctaaa
     9481 attagcaaaa ggttactacg gttcaaaaca cactttattt aaaacagcaa aagaacaagt
     9541 aatgaattca tacaactacg catatcgcga tcgtcgtcaa aagaaacgcg acttccgtaa
     9601 attgtggatt gctcgtatca acgcagcagc tcgtatgaat ggcttgagct actcaaaatt
     9661 aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt aaaatgttag ctgacttggc
     9721 tgttaacgat gcagcagcat ttactgcatt ggctgaccaa gcaaaagaag ctttggctaa
     9781 ataagctaaa caattataaa gaggaaagaa gataacgaaa agttatgttc tttcctcttt
     9841 ttttatttac aatcctccat caaatgaaac aggacaataa cgtataagtc taaaataaca
     9901 taattaaggt tttttaattg aaaactgaac atttatggtc atcctttaat gaaagcgcta
     9961 actacataag ttagaatgag tttgactgat atattagttt gcttatcaga aaaacaaaat
    10021 ggacggagga ataacatgag tgatattatt attggtattt tgttagttat ctcattcttt
    10081 ttcatggtct ggtactgtgt aaaaggctac aacttgatgg tcggcttttg tcatcatggc
    10141 tactgtatgg atgggattag cgctagtcgg aaacacgttt tcaccaaact cggcgatgga
    10201 agggcaagga gtaattgatg tgttgacgca tatctatacg acggggccag ctgagtacgc
    10261 caaatcgatc ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc tagtagatag
    10321 cggaatcgca gctaccctga ttcgaaaggt agttgaactt ggaggggaca agccaagaat
    10381 cacgatgagc ttactatgta ttgtaaccgc tgttatattc atgtcgatga caggaatcgg
    10441 accagtgatt tctattgcag ttattgtgtt gccgatcttg atgtcattag gtatttcagt
    10501 accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg ctaatatcgt
    10561 caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg aaagctatac
    10621 ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag ttgtggtact
    10681 aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag caaacgtacc
    10741 agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac cagttttagg
    10801 ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta tctgggcatt
    10861 gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt ttgcgaaatt
    10921 atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga ccttagcgat
    10981 gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg gagattggat
    11041 accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac taggattttt
    11101 ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg tgttagctgt
    11161 taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa cgattgctcc
    11221 tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata caaaagtaac
    11281 ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg cgatcttatg
    11341 cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat aaaaacgatt
    11401 agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga tgttggcggt
    11461 acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg aaaatcttca
    11521 gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca gtcttttcaa
    11581 aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc acattctacc
    11641 acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt aatcgggatg
    11701 gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa cgatatcgat
    11761 ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa aaacttaaat
    11821 gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga ggtcttggtt
    11881 tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta tgaagcagca
    11941 tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata tggattgaca
    12001 agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct agatacggca
    12061 acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat gattatgcgc
    12121 ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt aaccatgctt
    12181 tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc taatggtgtt
    12241 tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg acgtccagca
    12301 atcgattatt caattgtagg tggccaccca acatatatct cttcattaga tgttcgggtt
    12361 ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat cgatgtcggt
    12421 ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac agaaaaaatc
    12481 aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga ttacgtcaag
    12541 gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc caatgttctt
    12601 ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg aaaagccata
    12661 caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca gatcatggaa
    12721 aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta tcacttggag
    12781 aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat tacttacttt
    12841 agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat ttcttctatt
    12901 ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc gccaagtaaa
    12961 gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc aggcgctaca
    13021 ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt aacggctatc
    13081 gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac aacagaagaa
    13141 gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg cttattggaa
    13201 aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc tggaagtctt
    13261 cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc atgtatgaaa
    13321 acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat ggcagtttat
    13381 caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag agtctctgat
    13441 tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt atctactatg
    13501 gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata agttagaaga
    13561 gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc agtttcggcg
    13621 agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac aacaatcaat
    13681 cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg ccgatgtagg
    13741 agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc aaataaacga
    13801 acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga cggagccgtt
    13861 agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc aagctttgat
    13921 tattggacat gaactatttc accacatcga agcttctgtt aagggaattt atacgcaaag
    13981 cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca ttcgtgcatt
    14041 gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt tttcgcctta
    14101 tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag ggatcttaga
    14161 agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca aataaagaga
    14221 ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc tcctgtcaac
    14281 tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg aaattgttcc
    14341 ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt tcaaaagctt
    14401 gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt tacttggctg
    14461 ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg tgtgcaatca
    14521 gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat tttgccgttt
    14581 atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga tgggtctgtc
    14641 ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct cctcgataat
    14701 catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat caatcattga
    14761 ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg aaatcgtcaa
    14821 acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat cccaaatttc
    14881 tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat caaaaaaagc
    14941 ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt ctagtttagc
    15001 ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt ccaacttttc
    15061 ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat aagtgccgga
    15121 ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt ctcgaatcgg
    15181 tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac ttccaggtgc
    15241 taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt gcaaattatt
    15301 gataaccatt taaccttcct ttctatgcag atataatcta ttataacata aatcgcttac
    15361 aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca tgcctattag
    15421 agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg gtttgacacg
    15481 accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa tgagcagatt
    15541 gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt gcattacttt
    15601 aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta ctcgacacaa
    15661 tacaagaaaa atgcttagac ggtatttatt aagaatttac atcatccaaa gaagcgtacg
    15721 cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat cgttgcgaat
    15781 cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg tcttcagttc
    15841 ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata gttgggtgga
    15901 tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact tccagaacgg
    15961 ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt accaataagg
    16021 ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc cccatttgat
    16081 ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg actcatgcct
    16141 cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct tcgctgattt
    16201 gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa ctatgaagat
    16261 aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat cctgtgatca
    16321 tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct aaaggattgg
    16381 tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg agcacagcac
    16441 cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc ggtagagaac
    16501 tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga tagggtagca
    16561 aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc cattgttctt
    16621 ttgattgttt ttctagtact tgtttgaaac gttccaaact cttagaagaa gtacccaatc
    16681 cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg tcagtatccg
    16741 tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga tttttttcat
    16801 cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca ctcataatga
    16861 ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat gttcctttat
    16921 gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt atcagcatcg
    16981 aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca gataagaaaa
    17041 ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat gaacacgaag
    17101 acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa gtagaagcac
    17161 gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc gagcgcgatg
    17221 attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca ttgaaacata
    17281 tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac tatgcagggc
    17341 atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg gatgtcgttc
    17401 ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat ggaaagatct
    17461 ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat gcgatgaaga
    17521 tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc ggcagcgatg
    17581 aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa ccagattttg
    17641 gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat gtttctttag
    17701 ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt gtttccggac
    17761 ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca agtgcagatg
    17821 cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca gtaagcggaa
    17881 cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc gcacatggtg
    17941 caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta gataactatg
    18001 aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc catgaagatt
    18061 tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa ttgacaatga
    18121 atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc atcaatatga
    18181 atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc gaacaaacag
    18241 tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca cattacgtac
    18301 caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac actggagaaa
    18361 aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa cgtggcgttg
    18421 cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac gaatttatga
    18481 gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat cgtttggcga
    18541 aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat gtttaaaaat
    18601 gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt ctcaggaggg
    18661 atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt acgatacgta
    18721 aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata cggcgttaca
    18781 gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg tttacgataa
    18841 gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac ttttggatac
    18901 atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt gtcgtttcct
    18961 tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt caatatgatc
    19021 gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca aaagataccg
    19081 gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat taaatactta
    19141 tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg tgttgtcaac
    19201 aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa ttgttggttg
    19261 gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt caaaatccaa
    19321 atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc aagcgaaatc
    19381 aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct cgtcaatcca
    19441 atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact catccctagc
    19501 agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc cgccatagac
    19561 atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat catcaaatga
    19621 tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga aggatacatt
    19681 ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga gtgctgcgga
    19741 atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac aaaagcaaaa
    19801 aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt tttctctttt
    19861 atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa caaacacata
    19921 ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa gcataccggg
    19981 aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg cactgccgat
    20041 agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga tttttttcat
    20101 tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcataccat aattattcgt
    20161 gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc agaacccgaa
    20221 cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat ttcttgccca
    20281 tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat ttttatgcca
    20341 cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg ggagtagctg
    20401 attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag ttctagaaat
    20461 ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat aacgagccat
    20521 caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt gaaagaatgc
    20581 ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc cggttactac
    20641 ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta cgtaaatctg
    20701 attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta aggaaaataa
    20761 aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga ttggaaaaac
    20821 cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga tgtttctttt
    20881 taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt gaaaaatcga
    20941 aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa atagcttgac
    21001 cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca tagattgggc
    21061 gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca gataaggaag
    21121 ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag agaaagcctg
    21181 tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa tcgattgtaa
    21241 aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa tacttccatc
    21301 agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct gtctgccaat
    21361 agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg caactttgtt
    21421 ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa ccatggctaa
    21481 accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa aagcaaaacc
    21541 tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct tatgcataaa
    21601 aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt ataaattcaa
    21661 ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg ttactaatga
    21721 aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc gtgtaataaa
    21781 gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa ataaaaagaa
    21841 gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca ttttgatgat
    21901 catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc taccttcagg
    21961 acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta aagcatgaat
    22021 cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt tgacaaaaat
    22081 tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat ataagtaagt
    22141 aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat tttcatcaaa
    22201 ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag aacgtaattt
    22261 ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta ccatcaaata
    22321 ataatcggca tcaatatcaa aaaacatttg acggattaca ttcccttttc cttgacgtgg
    22381 ttctttcttt acgattgctc cgccttgaac ggccaattcg tatgttcgat cggtcgaatt
    22441 attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt caataacagt
    22501 tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt cacgtgattc
    22561 catttgtcac tctccttata taataaaact aatcttattt caataatacc gtataataac
    22621 tacaaatact agctattcca agaggaaata gccctatttt tataaaaatc acccactata
    22681 aaagaaggat aggatacctc tcttttagat agtgggtgcg tattcgttat ttcatttttt
    22741 tgacgtatta atctattgcg cccttttttc ttgaaatgtc taatacggtt cctgtatgag
    22801 catctgctaa aaattcataa taaacagttt cgccatcttc tatccgggaa agcccacccc
    22861 gataagcact cgaccgaacg gcgaatttcc gaataggttc tttttcaaaa gagatccaag
    22921 caccttcgat tgcgccttct tttaaaaaag atgttttgat attttcaagc acttggtctg
    22981 ctgataacac ttttttgcga tgataccaaa tcgttgaagc taccccactc aataatccta
    23041 gaccgatccc aacggctaac ccgcctttaa aatagcttaa ttcgctttct tcatacatgt
    23101 ggatatacct ccttcttctc acttgctact ctttgtgttt attttaccac actctcatat
    23161 tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata cgatcggtct
    23221 gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt ttagtctgaa
    23281 atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag ttaacagaat
    23341 tacaaggaac aagcggtttt gaacaagacg ttcgcgccta tatggaaagt catatggaac
    23401 cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttgggctg cgccaccatg
    23461 aagaagcgga tgcacctcgt gtaatggtcg cagcacatat ggatgaagtc ggattcatgc
    23521 tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcgga tggaaccctt
    23581 acgttgtttc tgctcaacgt tttacactta aaacctctac tggtaagaat tatccatgta
    23641 tttcttcttc cgttccccca catttattac gaggaacaag cggacaaaaa tcagtagaag
    23701 taacagatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg agttacggtg
    23761 ttcttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc aacgggaaga
    23821 atatcatcag caaatcgtgg gacaaccgct atggctgtac aatggtatta gaagctttag
    23881 aagcgttaca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat gtccaagaag
    23941 aagtaggctt gcgtggttct aaagcttctg tcaataaatt caagccagat ctgttctttg
    24001 cagtagactg ctctgcagca gatgacacag tgacgaaaaa tggcacattc ggacatttag
    24061 gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct cgtttgcgtg
    24121 agtacttgct agatatcgct gaaacaaaca acatccctta tcaatacttt gtttcaaaag
    24181 gcggtacaga tgcaggtgct gctcatacac aaaatgaagg aatcccaagt acagtgattg
    24241 gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc gattttgaag
    24301 ctgcacgaga aatgttgatt caaacattga aaggtttaga taaatcaaca gtgaacacga
    24361 tcgtatacgg gaaataacgc gatagtgaaa ctataaaatc tggcaaaagc aaccttttgt
    24421 cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag gtgtataatt
    24481 taattgaaaa atacataaac agaaagtaga ggaaaaagaa tgattatccc aaatagttta
    24541 gaagaattag caggctacgt agaaactgga aaaagtgtat ttttctttac tgccggctgg
    24601 tgcggagact gccgttttat caaaccgcaa atgcctgaaa tcgaaaatga cttttctgat
    24661 tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga atggaacatt
    24721 tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg tctagtgaat
    24781 aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaat aaaaggctga
    24841 catctgttag gaggggtcaa gatgaatcaa gaatataaga acattttagt aggtattgac
    24901 ggtagtgaac aagcacttgc tgctttcaaa aaagcgttag aagtggctcg ccgtaataat
    24961 gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataattttat gggctatgct
    25021 cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaagcaaat gatcgaagaa
    25081 tgtaaagaat atggaaaatc tgttgattac acaaatatag aaggaattat tgcatacggc
    25141 tctgttcgtg aatccatggc agtcaaattg ccaaaaaaat atgcaatcga tctaatcatg
    25201 gttgggcagt ctggactaaa tgcagtcgaa cgctttatga caggaagtgt agccagctac
    25261 gtcatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga acagccaagt
    25321 aattaagcaa gtggaacttg tataaccttt ttaatcaaac agtaaaggga ctgtgacaaa
    25381 agtcttgtca cagtcccttg ttacgaataa atggtgttcc agaagaagct tcttcggaaa
    25441 taaagcatga aaaaaatctc atgcagtgaa tgcaattttt tgttaagata aatctcagca
    25501 aaggataatg gaggaaagaa catgattttt gcttataaca aagcacacgt cggagacact
    25561 ttgatggtta tcatagccga cgataaagga acagaaaaca ctgtcgaacg taaatggaat
    25621 gttgctcgga tcaaagatga acaaggtcaa gttgttgctt ggaacttttt ccatatctcc
    25681 gatcatctaa caattgaagg gaacggccaa gtaagcttaa cagatgaaga attagcgaaa
    25741 ttgaatcgat tgattaaaga agcaggattc gaagaacagc ttgaagctga tcatgaaccg
    25801 aagatcgttg tcgggtatgt aaaaacatgc gtcccgcatc ctgactctga ccatttatcg
    25861 attactgaga cagaagtgga caatgggaca gtattgcaaa ttgtttgcgg tgcaccaaat
    25921 atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag ccatgatgcc agatggaatg
    25981 atgatctggc caggaacttt gcgaggggta gccagctatg gaatgatttg ttctgctcgt
    26041 gaattgcatt tgccaaatgc tccagaaaaa aaaggaatct tagtattgcc tgaggatgcc
    26101 gtagtcggag aagtatttcc aaaataaaga ggttgtgaca agtctttgtc acaacctctt
    26161 ttttattaat tttcactatt ttgattttgt tttaatgccg attgatcgat cgtcaattca
    26221 acagtagttg ttttttcttt cgagccttca tagtaagtga ttttgacaga atctcccact
    26281 tttttcttat acaaagcaga ttgaagttct acaccagaag aaacttcttt gtcatcgatc
    26341 ttagtaatga cgtcgtattg tttcaaccca gctttttctg ccggtgtagc tgtttggaca
    26401 tttgtcacga tgacaccgtt agttacagat gaaggaatct tcaagatttg ttcttgttgc
    26461 tgagtagaaa cggcagataa gtcgaccatt gtgataccta gagctggacg tgtcactttt
    26521 ccgtcttttt ccagttggtt gatgatattc actacatcgt tacttgggat ggcaaatccc
    26581 attccttcta cgcttacatt agaagtcgat tcagaagtac ttgcgatctt acttgagttg
    26641 ataccgataa cttgtccttc gatattcact aaaggaccac cagaatttcc cgggttgatc
    26701 gctgcatctg tttggatcgc attgatattg actgtttcgt tcgattcatt cgtacttgtt
    26761 acttgtcggt tcaaggaaga aatgattcct gaagtgacag aattcgcata ttcagatcct
    26821 aatggggaac caatcgcaat cgcaggttca ccaaccttca aagcacttga gtccccaaaa
    26881 gaggcaaccg tttcgacttt gtctgaattg atcttcaaca ccgctaaatc tgaataggca
    26941 tctgttccaa ctaattcagc ttttaccttc gtaccgtctt tcatcatgac ttccaaacct
    27001 tgctgtccat ctactacgtg attgtttgtg actacataag cagtattccc gtcttttttg
    27061 tagattacgc cgcttccttc actataagct tccaaattac tatcatctga gctgctactt
    27121 tcttcttgct gcccgaataa tccgccaaaa ccgcttgatt ggttttgact ttgtaagttg
    27181 ataacagaaa cgacagcgtc ttgaacttta tctaccgctt tggtgatatc agaatctaca
    27241 ttgactttga cattttccac aactgtttca ccggcagaat tttgattgcc gcttgtagca
    27301 gtagaactat tgttgccagt gcccatcgct gcataaaaaa tgccagctgt cagtagtcct
    27361 ccaacgatcc ctccaacaag ccccagaccc aacttacgcc agaggctatt atttttattt
    27421 tttttcatct ttggtgtcac attttttcta tccatgtgtt cctcctccaa ttacattcat
    27481 ttatctactg ttagtatagt cttatttgtt gactttagtc tactttgaga ctttgaactt
    27541 tttatgaaaa actttaggaa aaatcattgt tttcagatga aaatatttga ttttcttttt
    27601 ccacagatat tgtgtaaaac tacgcggata ggattggaaa aataagggat aaaacatatc
    27661 cacagcctgt gtataaagtg gataaattct tataaaacgt atgtttgcct aatgaatgtg
    27721 atgaaataag gtaaaacaac gctgttttct gtggataaac ctgtggatgt tgtgcataac
    27781 tgttggggaa atctttttaa caaaaatagt caaaataaac atatagacga aaaaattgat
    27841 tataataaac aaagtgagcg tgaagatcaa aggagagaaa acatgaatat caaaattatt
    27901 tcagtaggaa aattaaaaga aaaatacttg atccaaggta tcaatgagta tgtcaaacgg
    27961 ctgaatgcct atgcgaaaat cgaattgatc gaagttcctg acgaaaaagc cccggagaat
    28021 ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag gagaacggat cttagcgaaa
    28081 atcaaagaac aagaatacgt ctatggctta gcaattgaag ggaaaaatcc cacaagcgaa
    28141 tcttttgcta aacaaatcga ccaattaggt atccaaggaa aaagccacct tgtttttgtt
    28201 atcggcggtt ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc tcagatctct
    28261 tttggcaaaa tgacgtatcc acatcaattg atgcgcctga tcctcgtcga gcaaatctac
    28321 cgtgccttcc ggatcaacgc gggggctcca taccacaaat aatgatattt tttaagagaa
    28381 aaaattatcc ctattttaaa taatagcaat aggtagtggt agggagaaat ttataaataa
    28441 cttttttatt tgctttggta gccaagtaag ttgcaaaaga aaaatagata ctgttaaaaa
    28501 gataatgtag acatagaaac aaatacgtaa aattttagtt agaataatgg caaggagaaa
    28561 aaacatgata gatgaaaaaa agtgtttaaa aaatctattg gaggatatgg atgatattga
    28621 agaaaaacta tctaaatgga ctaggaaaac aaatatattt cagatactta agctatctaa
    28681 aaatgaaata agacatagta attttttagc ttttttgttt gctccaaatg aaacccacaa
    28741 tttatctgat gagtttttta aaatgttttt gaaaaggtat atagattcta atgatgatac
    28801 caaagttgct atcaattatt ttgatgcttt acttaatagc tacccgtggt gctagttaat
    28861 tttaatatcc taaagaatat ttcaaagttc cttcaaaacg tcgttgtgct cgacttaata
    28921 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
    28981 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtctca actgtttttc
    29041 tttgttttcc aagtaa
//
LOCUS       DAPBBG010000035        28583 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPBBG010000035 DAPBBG010000000
VERSION     DAPBBG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.035"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..160)
                     /locus_tag="RD477_001759"
     CDS             complement(<1..160)
                     /locus_tag="RD477_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634116.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLD"
     gene            complement(183..989)
                     /locus_tag="RD477_001760"
     CDS             complement(183..989)
                     /locus_tag="RD477_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634117.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRITKENI"
     gene            complement(1000..1644)
                     /locus_tag="RD477_001761"
     CDS             complement(1000..1644)
                     /locus_tag="RD477_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634118.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(1660..2865)
                     /locus_tag="RD477_001762"
     CDS             complement(1660..2865)
                     /locus_tag="RD477_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDT7634119.1"
                     /translation="MENQNESLSKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            complement(2865..4889)
                     /locus_tag="RD477_001763"
     CDS             complement(2865..4889)
                     /locus_tag="RD477_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDT7634120.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFVKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(5052..5645)
                     /locus_tag="RD477_001764"
     CDS             complement(5052..5645)
                     /locus_tag="RD477_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634121.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(5646..5978)
                     /locus_tag="RD477_001765"
     CDS             complement(5646..5978)
                     /locus_tag="RD477_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634122.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(6030..8549)
                     /locus_tag="RD477_001766"
     CDS             complement(6030..8549)
                     /locus_tag="RD477_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634123.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKS"
     gene            complement(8546..11158)
                     /locus_tag="RD477_001767"
     CDS             complement(8546..11158)
                     /locus_tag="RD477_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDT7634124.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            complement(11158..13212)
                     /locus_tag="RD477_001768"
     CDS             complement(11158..13212)
                     /locus_tag="RD477_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634125.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            complement(13284..13586)
                     /locus_tag="RD477_001769"
     CDS             complement(13284..13586)
                     /locus_tag="RD477_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634126.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            complement(13604..13846)
                     /locus_tag="RD477_001770"
     CDS             complement(13604..13846)
                     /locus_tag="RD477_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDT7634127.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(13843..14310)
                     /locus_tag="RD477_001771"
     CDS             complement(13843..14310)
                     /locus_tag="RD477_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634128.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            14497..14709
                     /locus_tag="RD477_001772"
     CDS             14497..14709
                     /locus_tag="RD477_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634129.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            14823..16880
                     /locus_tag="RD477_001773"
     CDS             14823..16880
                     /locus_tag="RD477_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDT7634130.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(16918..19017)
                     /locus_tag="RD477_001774"
     CDS             complement(16918..19017)
                     /locus_tag="RD477_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7634131.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(19318..20091)
                     /locus_tag="RD477_001775"
     CDS             complement(19318..20091)
                     /locus_tag="RD477_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634132.1"
                     /translation="MRNRKWLLVCAVALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTI
                     DDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLY
                     PKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(20107..20850)
                     /locus_tag="RD477_001776"
     CDS             complement(20107..20850)
                     /locus_tag="RD477_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7634133.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIEEVQ
                     KDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEENRKQ
                     KLSWKRLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(20863..22839)
                     /locus_tag="RD477_001777"
     CDS             complement(20863..22839)
                     /locus_tag="RD477_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7634134.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVINGTGAEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDIQLTYNMFVNDSAAIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(22892..23563)
                     /locus_tag="RD477_001778"
     CDS             complement(22892..23563)
                     /locus_tag="RD477_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDT7634135.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(23649..23888)
                     /locus_tag="RD477_001779"
     CDS             complement(23649..23888)
                     /locus_tag="RD477_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634136.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            complement(23955..24194)
                     /locus_tag="RD477_001780"
     CDS             complement(23955..24194)
                     /locus_tag="RD477_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634137.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(24308..24649)
                     /locus_tag="RD477_001781"
     CDS             complement(24308..24649)
                     /locus_tag="RD477_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDT7634138.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(25268..25600)
                     /locus_tag="RD477_001782"
     CDS             complement(25268..25600)
                     /locus_tag="RD477_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDT7634139.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(26447..27487)
                     /locus_tag="RD477_001783"
     CDS             complement(26447..27487)
                     /locus_tag="RD477_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDT7634140.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT         8236 a         5676 c         4349 g        10322 t
ORIGIN      
        1 tatctaaact ctttctacca ttcttcaact cagatattgt tgtgttagat aaattcactt
       61 cttgacatat ttctttagct tttacaactt tgagataggc tttaatcaat actttctgct
      121 cagcgaaagt atctttgccg accaagttcg ctcgcttcat ttctttcact ccacctgttc
      181 tactaaatat tttcttttgt aatacgatca attttttctt gaagtttctg aatgatctct
      241 tcttgatctt tcatttgcgt tttcgatgtt tcaatttctt gctggtaact attaatctta
      301 ttttcagtat tttcctgatc gtctgcgact tgataaggca attgatcttt tagtttcttc
      361 actaattctt cattcaaatc aatggttgct tggaaggatt caatttgttt ttgtgctgct
      421 ttttgttctt tcttgtatcc tttcactttg taattgatcg catgttgttc ataatcttca
      481 tctttcactt gatctttaac cttatcttcg tgaacgtaat aaatcagatc ttgtggttct
      541 tgcatatcca cttttggatc aattttttca ggaataatgg ttaaacgcat ggcgatatat
      601 tcttctggta catgtgctaa cttcactaca aaataatcat cagacacctt gatcactttg
      661 gcttttattg cttgataatc ccctttagct gtctttactt ctacgcgata attaagatta
      721 gccagttctt ttaataagct ttgatcttca tcgtcgatcg caaattcaaa aactaattgc
      781 tggttcgctc ctttttcttt tgaaaccatc gtcagttcct tcgtgtttaa gaatgcgttc
      841 gtgcttacct catctggtat gtctaccttc ttatgcgtat aaatcggact agcaagaatt
      901 aaaaagccga gaatcataaa gattcccagc catctaagat aacttttccg ttttttgaaa
      961 cgttgtcttg ctcctttttt cttactcatt ttctccctcc taacgagtga aataactaaa
     1021 gtctgtgact ttccattgac catctacttg tttttcagtg acagttaatc caccttttat
     1081 tttttgtggt ttgacatctt taaattgagg attgccgatt gtataatcaa caaatacgac
     1141 aaaattttgt tcctctttta cactgcttgt ttgagcataa gtttgcacgt cacctaatgt
     1201 aaaggagtaa cttccgtaat catcatgttg gtgatcatcg tctgcaggac ttaatttgtc
     1261 gatcacttct tgggttgcga catcttttag tttttccgtt aattcatttc tacttaatga
     1321 tttatcaatg gccaattgac cgaactcttc tgccgtcttt ctttgtgctt cagttacttc
     1381 ctcttttgct tttgtgccat cttgttgctt cagctgatcg atttcttggt ttttctcatt
     1441 gatggtttga tttaacttgt cgatcttcgc ctgtgcctca gaggaatttt ttttgagctt
     1501 ctcagacgcg tttttagcca cttcttcaat tttaaaggta tttgtctctt gttcttcttt
     1561 cagctgccaa aacaacgcga tattgccgat taaactaacg ctaattgcag ctgttaagac
     1621 gatttttaaa gttttatttt ccatattttt tctcctgatc tattttttgc tgctattcaa
     1681 agatggtaat ggatcaatcc atgtaccatc gtctgtgaat gcgtgtcctt gtgcttgacg
     1741 ccaatccatt cgagtgaaac ctaaatgcaa gtgtgctgta tctcgtatcc cgattacttg
     1801 accaactttc acttgatcgc caaccttcac acgagaattt cctgtactat tcgcaaattc
     1861 ttgatatacc atattcaaac cgtcgtaatt aatcacgatc acacatgcac ctaaacctga
     1921 aataccagga ttgccaacgt acacgacttt tcctccatgc accgcgtgaa tttcacttcc
     1981 tggatgatcg actgaaccaa agtctaatcc atcatggaag ccattcggac ggaattcacc
     2041 gcctggattt gttccaaata attgaccacc actgaaggaa cttttatcta aactgctgcc
     2101 agggaatgga ttcgtccatt caccagtgat cgtgccgttg ttattgccac cttcacccca
     2161 atgtttgatg gcgaattcat catattcggt taatttatta gaagtgataa tggacataca
     2221 acctgtataa tacggactat cttcagtaat tcctgcagcg gcataaccac ctcgtcctaa
     2281 gatcaataag cttgcgccag ggcttttatt tcctacacat gcattaaaat tggattgtgt
     2341 ggctaccata aagccgtaat aggagaaggc tgcttttgag ttgggaaact tcatgtacca
     2401 gccaccttca ataccaccga ttcctcgcgc agttccttta ggaaacagac aaccatcaaa
     2461 ccaagtaatc ccaaaatagt tgagatcatt tcttgcggac gccgacgtac caaacgctga
     2521 ttcataaccg tattgcgaaa ggatcacact tggtaataat ttatatttca ctgctgcacc
     2581 tacagcatat tgcgcaatct catcagaaaa agggcctctt cctttgattt ctttataaat
     2641 cggtaagcct tccgaatatt ctcctgtaaa agcttcgcca ccactacttg aatctgaatc
     2701 agaattatca atcgaacccg ccgcaagtat tagaaaaagg gtcacgaaca cagtaatcaa
     2761 aatagtaatc aaacctgcag aagtaccaaa caaccacaaa aataagcgtc gctttgcacg
     2821 acgcttaaca tactgcttgc ttaaactttc attttggttt tccattattt tcctccatca
     2881 aacaatttta attgacgttt gttcggacta aaatgaacac gaatggtttc ttttgagcca
     2941 aaattgatca cagcgtctcc ttgattttgt tgaggaatgg attctaattc tgagtttgtc
     3001 attgatttac caactaatcc ttctatatga cgtaaaatag attcatccat acgcataaag
     3061 atcttcgtag aagaaaattc aaagactttc ttcagtttcg agaagttatc agaagacata
     3121 ttttctggca ccatttcttg tggggattgt gtagctaaaa cagtcattgc ttctacttta
     3181 cgcatttctc gttgaaagtt tgtgacataa tttaccgttt caataaaatc tggattaatc
     3241 gcattatggc actcatcaat catcgccaag aagcatttga agtcttgttc ttccatttgt
     3301 ttttctttaa ttaaacgtcg ttgtcttctt ccttgaatca atgcctgatt ccaaatcaac
     3361 gtcatagcca tgtacatttg acattgttgt acccctcgat ctaagcctgt aatagagtca
     3421 atgtcaaaaa agaccaactg ttgttggtca aggtgtcgaa tcgtactatg accatcaaaa
     3481 atcatgccat acgtctcaca taaggattct acgatggatt ccatttcttc ttttcgattc
     3541 tttttctctt gggaagcacc tggtaattcc agttgtccta aaaagtctaa aaattctttg
     3601 aacgtcggat aagcttctgg atctttacct gtgacttgcg tatcagtatt tgaacgcgaa
     3661 tttgaggaag taaagccgcg tggtactgtt ccatactctt cataaaaacg atttaagtag
     3721 gtcttaaatt ctgctttcat cgttcctttt agctgaggac tcactaactg tacctgataa
     3781 actactttgt ctaagtgcgt ttgataggac gctgcttcat tgatcttcaa atctttgact
     3841 gatccatcgt catataacct ggatgcttca tcatacgtcc gtgtggcaaa tatctctaaa
     3901 ggattgatca tcccgtcaga tccgtctaaa cgcaccataa taccgttata ttcctttaag
     3961 aaaggaataa agtcttttcc cttttcaaag ccccaaataa agtctcctgc agctaaatgg
     4021 gcttccgcga tcattttaag aacggtcgat ttaccagctc ccatcattcc tagaagcaag
     4081 gtattagtag acaaccgtac actattttta tacgtggcat tccagacaaa tgggccgcct
     4141 gtcatggttt ccccatattg tgtccctcct ggatctttta gcgtttggtg gtggaacggg
     4201 aaacctccgc caatgtttaa cgatggtagt gattgccctt tgatatttcc gaaccattca
     4261 gcttgttcat cgaacatggt aaacatcgat tgccaatctc gtttggtttt atgcagataa
     4321 gtcaccgcac gataatccat ccctttgaga ttttcaatca gttcctgcgc tcgttcatct
     4381 acttcttgaa tcgtgtttcc gtaaacatgt actctcactt tgacttgttt agaaatttcc
     4441 ccattagaag tcaattttcg ggcatattcc gctagttcca ggtattcttc cgcactttta
     4501 tttcggtcag tcgcttttcg ttcattatct gatcgatcat ttaattcatc taatgcacgg
     4561 ttgatttgat caatcacttt ttgcttttca tctgtgctaa catctagtgt ggccactgta
     4621 tgaggattgt tcatgagatt ggccaaccaa aaaagagtag gattttttgt gtagtgatag
     4681 actgtcacac atcgtgaata accatttcca taggtcacat actcttcatc gaaatttaat
     4741 ccaccttgtg gcatcacttg ttttacaaaa tctaaatcat accctttgga ttttaaaatt
     4801 tctatttccc gatttcctag ttgttttaat ggatcaagta ctttttgatc attttgtggt
     4861 gaaaaagctg ttttaatcgc ttctaacatg tacagtcacc tcttcttgtt tgattcttta
     4921 cttggttaaa agattgccct tttcccaact tacccaggct taaagtgtga ggaaattttc
     4981 tagtgatctc ttccttcgga acatcatttt ctaaccatat caacggatat ttcattctct
     5041 cttctccatt cctactcatc attcatattg ttcattcgat aaagaatctt gatcaattct
     5101 tttttgtaga gtggttttaa ccctaaaatg ccactagatc gcattaactg acgatttctg
     5161 gtttgtaaat ccttgattgt ctttccttca atgacgaaaa agaagcttaa gtcaggacgt
     5221 tcttgttctg ctcgtttcaa ttcctggatt tcctctacta atttcttttc atattttctt
     5281 aatcgctgta gatttcttcc taaggattgt tccatacgat tcttatctaa taattgccgc
     5341 ttttgcacga tttgttccgt tagatccgtc tctgtggtca aactaactaa cttaattgtt
     5401 ggcacatacg tacgcatgac ggtggtcaac tgattcattc gttctagttt ctcttgttcg
     5461 tttaatcctt ccacgtctgt agaaactaca cgataataac gttgaaaatg atcttcttta
     5521 gtaactaaga ctccctcttc tgtacaggat ttataaggaa ataaatccaa gatagatcgt
     5581 tcttctggtt ttccaaactg caaaatggat ttcttatcaa ctgaattttg tcctttgact
     5641 cgcatttatt catcctcctt atacgtacca tgataatttc ggttaggatc taacgcatga
     5701 tagtggttct tatccgtaaa gaaaatagat tcgataatcg caaaaatttg tcgttgacct
     5761 gggttattat agggtcgcca aacaatcact agagctatcg caatccataa gccataattc
     5821 aagagtgcaa aaaagagtgg taagtggaca agattattga ctattttcgc ccaaaaaaag
     5881 caaccaccaa gcgttaatag atctaaaaag aaaaaggcga aaaacttcca ttccccacta
     5941 aatcttggag gatttttcat gttaatatat ttatccataa atgatttctc cttttcatac
     6001 atagaaaaaa ggcctaactc gtaggccttt tatgatttga tatccttcaa taattgttct
     6061 ttcaattcat tgttcctgtt tttagtttct cttgttacat ttatttttgt tggtgcagta
     6121 aatgccacat ttgctggctt gtaacctgta tgaacgtttt gaataggttt tggtgcagct
     6181 tctcttgtca caacattttg aacggacgca ttccctgtct caccactttc agataaattg
     6241 taagaaacag ttgtcagatt tccattttca ggcaaacgac ttggtttcat ttgaacagta
     6301 cctgcaggtg attgatgaac aaaactttga tcattatcct tgaattgatt caaacgacta
     6361 tctttcattg gtaaattttg tgccttaggt aaaccaattt tcgttgattt ttcagttcca
     6421 acttcgattc cattatcatt tggcagaacg atggaatcct catccgtttg tacttcttga
     6481 tttcgttttg ataaatctga aatgcgtttt tcattttcgg agttaacaga tctataccga
     6541 tattgttctg gatcagtttt tggtaactgt ccctcatttt ctgctttgtc ttcttgattt
     6601 acttttgata aatctgaaat tcgcttctca ttttcaaggt cactgttagg tttatagcga
     6661 tattgttctg gatcagtttt tggtaactgt ccattttttc cttcttctga atttctttct
     6721 tctatggaat ttttctcttc aatcgaattt tcattagagt gcaacgcttc ttggtgatct
     6781 ttgactgaag gaatcactat accttcttga ttttcttttt cggttgttgt attagctttt
     6841 actgctgcat taacttgtcc ttcaccatac gcatctttta gtccttcttt gacttctgaa
     6901 gctttttctt tttgtttttc tagtgcttcg gatggattct tcacagcttc agccaattct
     6961 ttagctctcg tgccgtctaa tttctcttct aaggtgtctt tggctttttc tgtaagatct
     7021 tcagctgttc cttttaaccc acgttcattg acgtaaccaa gaccttctgc ggctttagaa
     7081 acaccatttt tcgctttccc gaacatttcg gaaactgttt tcttactatt aggtacagtt
     7141 gtagctttat cgtctgattg ttcttccccg ttatcagggg taacatcttt tcgatggcca
     7201 aatccatttt tacgaatatc atttaaatgg cttaaaccac tcttaccagc acttgcaagg
     7261 tttttagctc cgctacccac atttttcgca atatttccta aggcaaacat gctaagaaga
     7321 gaattactgc cgtttttcag actcgtatca acaccgaaaa tcttcgtaac cgattgagag
     7381 cctttgaata gcgcaatagt tagcacaatt aaagcaacaa catacaagaa tccattgatc
     7441 tttcctgccg catgcaaatc accgatacct gaaagcatta atgtatagat tcgtaattct
     7501 aaaatcgtaa acgccaataa taagaaggac tgagaaatat cttctatcgc acttttcatc
     7561 cgttgtcctg tttcaaagtc cgttgatgcg gcaatcacta aatagaattt cttaaatacc
     7621 agctcaataa tgcaagtaac aatcgtaaat aatatgaata aataggccac cgccaaagaa
     7681 gattcaccag ctaaaataat tcctggttta ctaatgtatc gacgataacc cgaagtgtat
     7741 acatattttg ctaacccttc atcactaatc tccacaggga tttctttccc attatcatct
     7801 agttccaatc ggtattgtaa tgcctttaac cgatcctttt gtaaatctga caatccactt
     7861 ttatcgttat ccgccatctc agcggcttga tcaggagtaa taatcccatt gatattcgct
     7921 gttttaaaat ttttcgctgt taaagcattt cttttatctg ttggtaggga ttctacttta
     7981 ttcgggtcag ttgctagtaa cgtactgaca tcgatcacat tattttgaat aatttgaaaa
     8041 gcaggtgatc ccgttttatc tgctttattt gctgcagtga cgtctccgta aaagctttta
     8101 ctgatattta acccttcatc gattaaagaa ctaccaccaa aaatcaagat catggccata
     8161 aataaattaa ccaagatatt tttgactttt ggtgggtgtt tactcataaa aaatttcaag
     8221 cctaaataaa ttaatgtgac gacacaaaaa agagccaata ccttactaat caaactttga
     8281 aatagtgcat ttaccccagc ggcgttcaat aagtctttta aatcaaagct ttgataaatg
     8341 gttttttcta gtaaagtatt gagattatat aatcctttaa tcaatcccca cagaaaagtg
     8401 ttccaaatcc aactaaatac atcttgatac tgcagataat taccataatc ttgtaagact
     8461 ttcgttacgg ttttggggtt tgcatcccct tctccgaata agcctgacca ccagtcggct
     8521 atgccagaaa aaattcctcc aatgatcatt ctttcaccac cttttcagat tccaataaca
     8581 accgtttccc aatcatttca gaaatttctt tatttttgat caacttttca acatattctt
     8641 gaagtcgatc tgctgcttgt tcttcgtaaa attgaattaa caatttttta gccgtctcat
     8701 tctcaagaga ttgaacgatt ttataaaaca tattttcttc gtttttttct tctaattgat
     8761 catgcagtcc atgatagaga tttctagcat attcatctgg taggaaataa tcataatcaa
     8821 tttgtaaagc cttaagatcc aagtccgcat gtgtacaatg tatgtctata tcattgatat
     8881 cccacgatgg attgaaatct tcaatatatt gataagcata aggaagattc gtttccccat
     8941 gattaaaaat aggatacggc cgtattttat gacctttcaa atcttgtcgt tttaaaggag
     9001 cgagtacaat cgtttcacct tctaaaaaag tagaaattct ttcaggagtt aagatacgat
     9061 ctgaatcaac attgcgattg atatgagtat tcatacctaa tgaattgatg gtactgttct
     9121 cttgttcaac cgtcttattc cccacttttt tagaaaattc ttcgatggta ttatcatctg
     9181 tcgacataat ataaatatgt gtctgacaat tttctttgat aatcgctgcg ttttgttctc
     9241 catacttcgc ataaagttgc tgataagatt gaataaacag atcccacaac atgtttcggc
     9301 cagctgtgac cgtcatgatt ccttccatat tatccattgg tggcatattc ccaaattcat
     9361 ctaaaataaa atgaatcctg cggtgacatt tatctccttt agtcttaaca cattgcgtag
     9421 ataattcttt atataactga ctaagaaaaa tactaacaat gatatggtta gatggatcat
     9481 aatcaggtgt aagcataaaa atagccattg gtttatcggt atagtgcatc cgaatagact
     9541 ctactaataa gttacttttc tcctcatcta atgttagaac ggcttctttt tctaattctg
     9601 gcaaatgggt ttctcctcgt tttttccgat aaacgatatt accctcttcg tctttcaata
     9661 ctctttgaga aaattcatac caagccacat attcttttcc ttgatgatcg tattttaaac
     9721 aaacttgatc acctgcaact aaatgacaat caaaattgat ttcacaaaat cctttatagc
     9781 ctactttgac acgttcttca tgaaatttct ttcccttatg ataaaattct actacgactc
     9841 gttgatttaa aagactttca tgtaatttca aatctaagta ttttggaaac ccaaccgatt
     9901 tataatcaat cgttgattga gaagtcatct ttgcaatttt cgttagagta aatggattca
     9961 atcccgtgat tgccgttgat aaaatatttc ccttcgcttt atcagatcca attttcgttg
    10021 ccccatattg tttttttgca atgtttccag gtggcaactg attcataaat tcatctaagg
    10081 cattcaactg tttgaattca ttgtctggat ctggataata attaaaaccg ccaatctcag
    10141 aaagaagctc aacgagatta ttcggtgtca atttggagta atctcctgtt tgatcagctt
    10201 gttctaacaa cgcaaaaata agcgcattga cagctttctg cgcgttttcg ttaaagtatt
    10261 tgttatctga agcgttggca tcataataaa tcgtatttgt gaaagtattg gttaactgtg
    10321 ttgcagtatc tatgtctcct cgtttccagt atttcacgat gagtgccaat ggattatcac
    10381 tcattgattg cagtggatcg gtgatattaa aggcatatac atcataccct ctagcttcta
    10441 gttcatcttt actaccaaca tataattctg tttttggatc attaacgacc attgaacttt
    10501 gtttttctgc acgagataaa ttgtcaatgg tggctaacac ttcaatctga cctttccctg
    10561 atcgagaggt accaacaata cacgtattaa cagtatctgt ttgaatgtac caatattttt
    10621 tgtaatgtgt gattggaata cctccataac ctggaaaagt agtttttgtt tcaggtattt
    10681 gcggataaat ctgttggatt tcttttaagg tcattaatcg attatcccct ttttgtccat
    10741 ctgctagagg acgaaaccac atgtccattt tcaatcccat aagtacagct ggaaccacac
    10801 aaactgtcga aattgctaaa cgtgtaccca aactagttgt aggagtaaat ggattccaaa
    10861 aaccagctga atcaaaatga aagacctgtg gcatttcctg taattgaaac agatttattc
    10921 cttgtaagct tcccttaaca atttccaatc ggttgaaaac ttctggtact cttgttacta
    10981 aatactctaa agggaggtat acaagtccac tataaaaaat ctttttcgaa atgcgtttgt
    11041 catgtttttc tggtagctct tctcctgagg gagtatagcg atagaaatga gaaagatacc
    11101 tattcttttc taaattgaat cgtttgtctt ttcgcttcgt tgtttctcta acacgcatta
    11161 tttcacctca tttgattctg aactttcgga agtgtttgat tgtacagttg aatcgcttga
    11221 tccttcttta gccttactag aatcactctc tttgctattt tccatgcttg actctgttgt
    11281 tgactgattg gtacgattca ataattcttc taattgttgt tcatactctt ttcttgcttg
    11341 ttcctgggct tcttccttga ttgacgccgt tgtggctaca gaatcagcct gtgaagattg
    11401 attgatatga ttaaaagcaa atactgttcc tccacctaga agcaagctta aacttgcaat
    11461 acatcccacc atccatccgt taaaggaacg ctggggtgaa ggagttaaat ttgcttggtt
    11521 gttcactggc gtttgaacag cagctactgg ttgaggttgt gctagataat tcaactgtgc
    11581 ttgacgcaat gtatccattt gttcttgacg ttccttacga gcagctgctt ctttttcctt
    11641 ttcttgctct aaccgttgtt tttctatcgc cattttttgt tccgcaagat cctgttcccg
    11701 ttttcttatt tctaattgtt cacgctcttt ttctttttgc cgttgttttt cttcttgttc
    11761 tttcaattct tgttgttgaa tttcatactc ttttttccat tttggaattt gtacttgtaa
    11821 ttcttgctgg atctgttcgt gtttttccat aagaacttca gtcgttgttt gatatagctt
    11881 attcagttgt tcatttaatt ctttgctaat ctttagctta tctgtacgta aaccgcggta
    11941 gaactcttct tttcgttctt taatacgttc ttttaacaag ttttcttttt ctttcgataa
    12001 cgcttcttga ttcgtcttag taaaattgtt gatcttttct acacacaagg aataagcatt
    12061 gtcgcgtttc ttttcgtacc gctctttgat tttttgtact tcttcttcaa tcactcgctg
    12121 ttcttcttta tttagttcct cttttacttt ctcccaatta gacgactgtt ccgtcgcata
    12181 ctcattaatt tcttgatctg tttcttgttg acgttcttca aaggtggcta ataaatcttc
    12241 ttgtgcaagc tcatgccaat ttgttgattg gacagactct aactgttcta aaagtgtgcc
    12301 tttaccctgt gagattaatt cttctttact ctctaaaaat gcttgatagg tttggggtaa
    12361 ttgatatttc gaataaagct catttaaaaa taatttgttt ttttctttca tccattcatg
    12421 ctctggcgga tattgatcca aaatataatc aaatacagtt gccttttgta tctctgtttc
    12481 gtttttttct acttcttcac tgataagtgg ttcaatatat tcctgagaat ccccttcagt
    12541 ccatgttgac tcttcattag cttcaactgg atcaacttct acttcgtccg tctcttcatc
    12601 ctccattgtt tcttcaaagg gatctacctc caattgaccc atcacatcgt tctcttcctc
    12661 aaatatcgat ttctgagtcg gaatatcagc ttgttcgtca acatagactg tctctttttc
    12721 ttttgactcc ttctttggaa atattggtcg ttcccatggt acttcttcat ttacttcgac
    12781 ttcactcact tgattcactt ttctttggtc gctctctaac tcttcttcta aaacttgtaa
    12841 aagtttttcg tttcgataag actcttccac actcatagtt tcatccgtat ctttaaacac
    12901 atcaaggcgg attaaatcca tcaatgtagg ataaaaacct tctgaaattt gataatccag
    12961 tgggccaaaa acgtattctg tgccagcctt ttctattttt ccttctttat catgtaacgt
    13021 gagagtcaat ttaacagaag attgattctc ttgttctaac acttgtccta acacatgtag
    13081 ctgttcttcc gtttcaatga cagaatattt aattgttttc ggtaaaaagg aagtacgctt
    13141 tcccactttc ttcgttgctt ttcttattaa ctctttatcc aattttgcat ttcctgaaat
    13201 cgtaaaaaac atgaatattc tcctttcata aaaaaaggag taaaaagtag ccacgtactt
    13261 tttactcctt ctacttgttt tttttatagt ccttgtgggg tttgtccaaa cattgagaac
    13321 attgaaacta caatagaagc ggcagctaaa ataactccta cccccaacaa tacataacct
    13381 acgtgtgtct tcccgttttc agctcctctt cgcccaaaga agaagaaaat acctccaaga
    13441 gcaatggaag ccactgccgc aacagcagca aaaatcatca gattattttt caaactagtt
    13501 gcggcagttt gtaatgctct aaaaatatca accgcagcta ctactttcgg ttgtgttaaa
    13561 aaattcattg taattgattc aaacataaaa ttgttcgctc cttttagtgt ttaataattt
    13621 catcgaaaac aagcttttca cgttcacgtt gatagatata tccttctaag tccttgtctt
    13681 ttccccgtaa taggtcaaat tgtgcttttt caaggacttc ttgtttccac tgtgtttcag
    13741 atacaccgcg acgttctaag attttagacg cggctttttc aaatggcgtt ttcgcttttg
    13801 ccactatgat tcctccttta gggtaaataa aaagactagc aatcactcgc tagtcttttg
    13861 taatttgtat ttatttgatt gtgatccatc atgaatcaat ttttctttcg ttcgcttttc
    13921 atccttacta gatccaagaa aaacaatttt ttttacgcca tctttctcgt aaggaacaac
    13981 gataaataaa gaatcgggtt tgtttataaa ttttctgata cctgcagaat ctactatttt
    14041 gtatctttct aacccttcat agtctttttg tctcttaact gttatttgta caggatctgg
    14101 atgccataca ctttcagaat attcccaggt agtatcatct ataattttta aattatgaat
    14161 aagaccacct acttcttctt tccaaacagt tcctggttgc aataaactat tatcttcttt
    14221 agcttccgat ttcttattac ttgaacatcc acttaaaata ataaaaaata ttgcaaacat
    14281 agagacaaac catagttgct ttttcttcat tttattcctt ctttcgttgt ttatttttag
    14341 tatatcaaat aacgaaagta gcaacaaacc tcatttggat acacggagaa cgaatactca
    14401 taaaaatcca cctttctata gaaaattttt ctcttgatat tatgatttat tacaaaaaag
    14461 agctataata aagaaaaaac aagaaggagc attgaaatgt acgctgataa aaagaaaatc
    14521 gaatggttgc tgtttgatag tggtaaatca gttcgttcta tatccataga atctggcgta
    14581 cccttaacta cagtcagtga ccttgccaac aaacgctcta gtatagacaa catgagattc
    14641 ataaatgcta tgaaattaaa taaattggct gaagaaatcg ttgatattaa tacttcaact
    14701 aacaaataaa ataagaaaag ccaaaattat atcaggcttt tcttattttt tattgactat
    14761 atcggttagc cgatatataa taatcatgta aacattttta attaaattta ggagttgaaa
    14821 aaatgaaatt atttaatcca aaaactgttt tagctattgg aacatttgct gcctcactta
    14881 gtcttgctgg atctgttgac gctgcttcaa ctaacgtcat gtatcgcctt tacaatccaa
    14941 ataatcatga acatttttac acctctagca aaaaagaacg tgaccatcta gtaaagataa
    15001 aatggggaaa ttatgaaggc cccgcctggg aagcaccgac tagcggtggc catcttgtct
    15061 atcgtttgta caataaaggt cttcgtgatc accattacac agctagttgg gatgaagtga
    15121 aatggttgac taaaaattac ggctggactt atgaagggcc tgcctggcgt tctgccgaaa
    15181 aaaataataa accgatctac cgccttttcc ttcctggtgt aaaatctggc tcacatcact
    15241 acactgcaag ttggaaagaa gtacaatggt taaccaaaga ctatggctgg aaatacgaag
    15301 gtatcggttg gtatggcgct gacacctcta aacccaaagt gaataaatca gctttagaag
    15361 ctctttatac gagcgttaag ggaactaaaa aaggtgacta taccgacaat acctggaacg
    15421 cattccaaac cgctttaaac aatgctaaga aagtgttaaa tgactcaaaa gcgactcaaa
    15481 aacaagtgga tagtgccaaa aataaattag attctgctta caaaggatta aagaaaaaac
    15541 cagttcctac tgtgaataaa gctgaattac aagctttata caaccgagta aaatcaactg
    15601 ctaaaggaga ttttacagaa gaatcttgga ataattttca gactgcttta tccaatgcta
    15661 aaaaagtact agacgattct aaggcttctc aagcccaagt aaataatgct aaaaatagtc
    15721 ttgatgctgc atttaaaggg ttgcaacaca aacctgaaac accaaaagaa tatacggtta
    15781 ctattaacca tattgacaat caaacaggtc aagtgattgc tacagatcaa gcaaaagtaa
    15841 cttctggttc tacttacact gctaaggcaa aagcatttaa atatagcgaa ggcgctaaag
    15901 ataattttag ctacaaagtg attggtaatg atactcaaag caaaacaatc acaggtaata
    15961 caacaatcac attcaattat aatcaagtgc atcaaatcaa tttgttgtgc aataacaact
    16021 acaaaaacga aggacgagtt aatattttga aagaacagat agtcaatgtc actcacggtg
    16081 acagtgtcac tttaactgct ccttctattc aaggatatgt tttagacgat cgggtagaac
    16141 caacaaattc agtaacctta aacaatgtga ctgaaagtca aaattatatt ttcaactaca
    16201 ctcgtaaatt taatgtgact gttaatcatg taaatgctga taccaacgct gttctctcta
    16261 gtgaatcaaa aacagtatac gaaggggata gctttgctac tccttggaaa aacatgactg
    16321 atcaaaatta tttcttgtgc agaaatgacg atcctaccgt taccgtggat aaaaacggcc
    16381 aacgatccat taataacgtc gatcaaaata aaacaatcac attcaaatat aaaaagattt
    16441 cattagacga attacgtaac tttatgcgtc aaaaagaact tgcttggtta aatgattatc
    16501 gccaacaaaa tggtgttgcc ccaatgcaat tcaatgatat tgttcaacag gctgcagata
    16561 ttcgagctaa agaattacaa gtcagcttca gtcactatcg ccctggtggt ggtactttcc
    16621 aagatttatt ggaaagctta ggctgctacg gtgctaaggg agaaaacata agtcaaactg
    16681 ggctgtatat tgatgacctt ttatcagacg atggtgctat cgatgccatg gtaggatgga
    16741 aaaatagccc tgcccataat gcaaatttac tttataataa ccaaagtatt ctcggattag
    16801 gtcataattt tgtagttgac tctgctggta gaattggcgt agataatgta tttattagtg
    16861 cgaatccaat cttcaaataa aagaaaacaa ggctgattct cagccttgtt tttatattta
    16921 tttgagtttt aaaactacat agcccaatga tgctaaagta agaaataata tacttccaat
    16981 aacagttagc actaaatgat tcgatccagt ttttggcaca atgatctgag gtaagttagg
    17041 ttttacccca atcgttacta cattagttgc tttctcaata ctttctactg aaacttttgc
    17101 ttggttgtcg atatccaatt cttcattcaa aggagtttta gctgtaaata tcagttgaat
    17161 ttctcgtcca tctaaaagat ttttacttaa gaaatcagcg gaagcttccc acttcagcgt
    17221 tcttgaattg ctatcatagg ttagtgtacc ttctgaagta atatcttttc ctgtactcat
    17281 atctatcact cgcgcagaac cttctttata cgtcaaacga tcttccactg gatcttgaat
    17341 actaaattgc ttgtaatgat ctgaggcaat gtctacacct tttgtatgcc atttttgtgt
    17401 gactagatat tgtacttcag atcctgcatt taaaggagct gtaggcgttc cttttttacc
    17461 atcatttcga ccatcaacat ctttcccatc ttgttccact tttttatttg gtttaagaga
    17521 gaaatagttt tctactgtat ttgttttttg tgttcctttg ttcggatcat tgttaaacgt
    17581 tacagtagcc acatttggtg caattccttt tgcatcattt tttgctctca tatggagctg
    17641 caaaatataa tccctaccgt aatttgcagc gttagctaaa aagctgtcag aagcagtata
    17701 agtgactgta ttacctgata cagttaactt tcctcctgta tcagcttttt tccaagtacc
    17761 attcgcttca acataccaca atgcgccatc atagtttttc ccatccgata ccatatcaaa
    17821 atttttgctg tcaaaggtat cagtgatttt aaatgatttg tatttgttca ttgtgttgac
    17881 tgttaatgca tcaaactttt ggtgaatatg atagtaaaca tctgatcctt gtggaatcac
    17941 ttttttgtca atattgtgtg ctgcctcttc agtattagct ttgttaaaca cttcttttgt
    18001 aggcttcgat ggttgtggtg tccatagcaa tgcagtatca aagctaaaca tcatactatt
    18061 cgtatttgcg ccaatcatga atgttgggga agcgccttga agcgtatacg ttacaccact
    18121 tctgtgataa gtatcagctc ctacaacatc ggtgaaatga tcattcgagc tataagcctt
    18181 ttctgcacgt cctcccacta cttttgaagt taaattgttt gttgataacg caggagcttt
    18241 gtattctgca catgctgtat ctgttgtgac aaaataaggt aagctaccca ttttttcata
    18301 cccaacaaat tctctcatat caatattacg ttcaccgaca gattcattat tgtcattata
    18361 ccaagacaca cgttgctgat actgtccatt taaactgtaa atggatagaa atgtatcgtt
    18421 taaatcaata ccagctgctc tcatcgatct catttttgat tgaatttctg gctgtggttc
    18481 actcaaagct acttttgttt ttgttcctgc atacttaaat tctagttttg cattcatgac
    18541 attgattgac tgcatgaaaa atcctcgata aatattttta tcaaagagca aataattata
    18601 atactttttg ccattaattg ttttagctaa tccgtcttgg ttaaccccag cgtgcgctac
    18661 aaaattagaa aaagtcacgt cacaatcaat ttcttttcct agataagtac ctactttttg
    18721 ataatgggca acgatttttg acgtactttt caaatctttg acttttggcc aagcaagtac
    18781 tttttgtttt ttagttgaat actcatctag tatcttaaat ccattgatcc cagttacttc
    18841 gatatttctc aatgaatcac tatcatgata aagcaccaga ttattatttt ctttgatttg
    18901 actcggatca actttttcta aggcctggac tgcagttccc ctcgcaaaaa gaagaaggaa
    18961 caaaaacaac actaaagtaa ttactcttcc cgtagtgcaa tgaattttat tttccataat
    19021 gccctccatc taatattttt ttaaacgatc gtaagctttt cattcttttt ttcctaatac
    19081 ccacgttacc aatcctgtaa gaagaaccaa actaaccata aaaattggat tgatcaagag
    19141 ctttttaatc acctctaatg gcatggaagc acattgaatg tttagaaaat gaatgaacaa
    19201 tcgttgatat cttggataaa ttgataattg agaaaaaatt aaaccgcaac acaatattgt
    19261 tgcggctaaa aaggatagat taaaaactaa tattcttatt attttcatca tttcatccta
    19321 attatcaaaa aataattgag caaaaaattc ccaacaattt tcaaaaaagt tcttctttac
    19381 accagttgac acaacttttt taatttcttc tttcggctct gatttctctg atttagggta
    19441 taaccaaaca tccgtcaaaa attcaccttt ttcatttgaa acaggtgtca ataaaacaat
    19501 atcttcactg tttgaaatgt tttcagggct tgattcatta acgatatagt atgcttgttt
    19561 tttgtttgca ggtacttcaa tggaaaattc aatcacgcct tcctgatcat caatcgtttt
    19621 ggtttctcct tcagccactt ttttcaactg gtcgtatgaa agtttttttg ctcgatcttt
    19681 caattttgac tcaattattt ctacagattg atttttctca ttatcatctt gtgacatttc
    19741 ttctaagata ttcgtgatat cgtagacaat aaacttcgcg ccatttactc cttcttgttt
    19801 ttcaagcttt tgcccctggt tgtctaattg ttctgaagaa ataatctttt gaacgtgaat
    19861 ggtctgtttg actatttgat cgtcttcagc aattttcggt tcttctgaag aatccactgt
    19921 tgacgaatcg acggtttcat cgctgctttg aatcgtgctg ctatctgacg tttggattgt
    19981 ttgttctgtg ctatccgtgc tttcttgact gtctgcaaac gttaactgtg tgtaggcagt
    20041 gaaagaaaaa agagcgactg cacaaacaag tagccatttt ctatttctca tgttggttac
    20101 cctcctttat tttttctttt tataaacaaa tagcgaacca atgatcatga ctgaaagaaa
    20161 aacagtactt cctaaaagcc gtttccaaga taatttttgt ttcctatttt cttcatcttt
    20221 taattctgat tttgtaaagg ttactcgttt cccatacact aggaaacggt gtgtgttgat
    20281 tcctttaggc gtacatgtca ttagcgttac tcgatcttca ttgggatttg gttctaagta
    20341 agtgacaaag tttctttgta gttcgtcttt ttgcacttcc tcaatccgat cgatttgata
    20401 agcgtgttgc tcaccatcaa ctttgatata aaacttttct ccttttttta ctgaaggtaa
    20461 atcatcaaaa agattatctt tataaagtcc actatgacct gcgattaaag gatttttacc
    20521 gtttcctcct gtaatatcac cagttccttc cgtaatacct acaccatttt ctaaaatttt
    20581 gtcggacgtt ccgtcataaa ttggtagttt taaagaaata cttggaattg taagaacacc
    20641 aacaggatcg cctaactcta ttttcgcaac atccaaagaa gaatctgttt tttcaagatt
    20701 ttcaatggct aaaattcttt gctgttgttg aaatttcgct acatttgttt ccacagatgg
    20761 aattaaatag actgctactg ccgacagcaa ccaaaaaaat atcatcagtt tgtgcatgac
    20821 tcttcttttt gggggcttac gaataatcat tagctgatcc ttctaggctt tcttcttaaa
    20881 gtaaacaact gcagaaagtg cccctgctcc tagcaacaag caagagatca atacatacgt
    20941 ttggaaacca ttactacctg ttaatggaat agctgctttt tgattatttt taacagtgat
    21001 tcgattttcc tctttaaatg agtttttgtt tactttaaat gcaatatctt ctgttaattt
    21061 ttggtaacca gttggtgctt ttgtttcttc cagtgaatat tcaccttcag ttaaaccagt
    21121 gatttcaaag tgaccttctt tatctgattt taggatcgct gcatttgcta catcgtctac
    21181 ccatactgct tctttgacac cgtctttatc cgtatcaact aatccaccat aatattttcc
    21241 ttctgcgttt ttaacagcaa attctgcgcc ttcaagcggt gttgctttga ttcctgttcc
    21301 aaatagtccg ccatcagctt ttaagaattt tgcgccataa gtcacagcgt cggctttcgt
    21361 tttttcatgg tgttcttcct gattgaatgg attgttgcta taaacgaaat ccacgctatt
    21421 tgttaatgga tttgcgattg cagcactatc attcacaaac atgttataag tcagttgaat
    21481 atcttgtccg tgtaaacttg cgacagtatc acttacttgg ccattattca cgataaaatc
    21541 gatcacaaag ccattttctg aattttggat cttataatct gtattttctt ttaataccac
    21601 tgatccatct ttatttgtta ctttgatatt tgctttagca gcgacataag aaagtccttt
    21661 acctgctgta tctccccact taaatacgct gtaaggagaa gtttcactct tcacaccatt
    21721 tactgtaatc ccatctgttc cacctgcaat atctgtaggg atatgaatcg ttccttgata
    21781 attcactgcg tcaccaattt ggaaagaatg ttcggcccct gttccattga ttactgtctt
    21841 atcgattaca ggagctttgt aatttacata ggaagctttt aagtcacttg tcacgccatc
    21901 attcgtaatt tcgaaggtta atttattgtg tgcatcgttg cgagcgtctg cgcctaataa
    21961 atattgatct gctgttggtt ctttatctga tcccactaca acttctgaag gaacttccac
    22021 aaaatagtat tttcctaacg tcaaatcagt agcagtcagc tttccttgat catccgatac
    22081 aagatctcct aattttgttc cttttccagg ttctcccttg tataactcaa attttgcccc
    22141 ttttaaaggt gtttgtttgc tttgagctgt gccatcgtca ccaaatttcg ttaattcaaa
    22201 actcaattta gaaacaatat ttttaggata taagtgaatg tcttttaaaa aacctgaacc
    22261 tgtattatca gtcattggcg caatcacaac cattggtttt gctttttgtg tgactaatcc
    22321 agcagcggat ttactttcat aaaccacata tacattccct ttggaattga cataggctgg
    22381 ttgatcagca aaagtgatct caccattttc atctacagct gaagtaattt tgttggttgc
    22441 attcttgacg tactcactgt tagatccctt gtcttctacg tctttcacga tttcgtccac
    22501 tttcgcgtta gttagatcag aatcttccct cggtaatact tgatccgtaa tatttcctaa
    22561 cgtaaactca acatcaccat aatctgcctt gttgtatttc gttacacctg taggatattg
    22621 atcgtgcgta taaccatcgt ttttgatccc gtcaatatca acattcaatt gcgttccttg
    22681 atcgtacatt aatttatgaa ccgtgatatt gaccttctct ggtggcgttt tttgcgtgac
    22741 atcttctgcg tgtacttgat acccctgaaa gcagcttgca atgattccta acattccaac
    22801 aagcattcca gcttttttta cataccgatt tattgccatt ttttactctc ctcatttcat
    22861 atttcaacga ctcgcgttga ttaaaacctt gttattgtag ctctccctgt accacgtatc
    22921 tttcttttcc ttgtgacgta taaacacaag taaacaacgt taataagggt ttctctcctt
    22981 tctttggtaa agacaagaaa gagttctctt caggaacgcc atcgattaat ttaaagtact
    23041 caggtacgat aaatttacgc gttaccttgt aacgatagac gccatcgtta ctaactaagt
    23101 ccatcacctc ccctaatgac aacttttgaa tatcagaaaa taaaacacct ggcatttcca
    23161 tgttatgacc tgctaggaca tagtttcctt tgcccatttc ggcatcttca taaaaataag
    23221 tagccgctcc aagactcaaa gtgtattgat ttgccccttt aaaaattggc aatgctaaac
    23281 caagtgcagg tatctgaatt tgtgcaaagg cttgtttatt gacacccatt tcgattgctt
    23341 ctttttgtat ggacaacatt tgccccaaag acatatcatt attgacgttc gttgcatcct
    23401 ccttataact ctttgcatgt tcattgattt gatctacggg catctttttt tctatatttt
    23461 gcttaaacac ctttccttca tgtttttggt agctaacata gccaaacaca ctgcaaaaga
    23521 cgattaaagc gactcctaaa aaaccaaata accattttct catccagcta ccccctttca
    23581 gacaaaacaa aaaatctttt tctcatttcc atagaaaaag ttttaaaaat gaattctatt
    23641 aatgaatgtc atgagacttc taacaatagt tgacaattag aacttttttt cacataagtt
    23701 cgtctcaact gttcaaagtt tttcattgaa cctaacaaac aagctttctc ttttcctgtt
    23761 atctgcatat cattgatata ttgcatgaca gataatcttt taaggtgttt ttgtttcatc
    23821 gtttttagca acttttcttc catttctttc gacacttttt ttctgttttc gatttcttca
    23881 taattcatga gttttcctac cttccttgtt ttttgcaaaa ataaaaaagc ctatgatcac
    23941 atacgctcta cacttcagtc ttgggttatc tttttctttc gtttaattac tggccgtgct
    24001 ggtttcaaat ccttgattcc attatgggtt ctccaataat cgaattcttt tcgtaatctt
    24061 tctttttcca tatctgttcc tttagacagg gatttgaata catctttagg tgaataacca
    24121 cgagctaaca gctgtacata ccttcctttt gtcaaagtat ccatatccat cgcctctaaa
    24181 actggtcgcg ccatctaatt ttcctcctaa ataaattcct tactcaatgc ctaaggtaat
    24241 actacctttt tattaatcgt cgtgactcct tctaagcctt tatctgtaaa atttattcct
    24301 ccagttttta ttcttcttta tccgctacaa attcaatgat ttcaacgctg acaatataat
    24361 cactcaaata tgaccaagta tcttcaacag ataattcgtc tccattatca ttcgtaagcg
    24421 tcattttaaa ccattcatta ccttcatcat cttcaggtag caagccgaat ccaattggat
    24481 tatcagtcaa tttttgatag tataaatcat cctcgtctaa tgctaaaaca tctaatcctt
    24541 tacaatttcc gcctcttcta aacgtcacaa atcctttgta atcccaacgt tggaaagaaa
    24601 ccttaatagt atgtgtgccc aatcgtatat tcggatcata atttttcatt tgtcattcct
    24661 ccattacgta aatttcattt tctctatgtt ttatatcaat tttttcactt tattcaggac
    24721 tcgttcatat tttatcgttt ttcgtttact taaaaataca taaaaaatat cgactcgttt
    24781 atggcgtccg aaacgagaaa ccctagtaga aatcggtaaa tgctcgattg atccaatgac
    24841 caacgtcctc gcaagtatct acatgtactg cagcacacga cacaatttct tgtgcgtcct
    24901 ccccattacg agaagaccta ctccttattc catgagtttg cgtcaaagcc tagatccctt
    24961 agtagctgct catgattaat ctaggcgcta ttctaacttg tctaacttct aaattccctc
    25021 ttgtttactc tgtgcatatt atttacacaa attcatttca ccactatcca aacaagggca
    25081 tttactcgat tgaccaattt tttacgttac caatcgtaaa aacctacccc gtcacccccc
    25141 gaaaggaatt gttgatagat caagtctact ccttattttt ctagtaattc taagttcttt
    25201 tagaagtctc gacaatattt tcaagcaagc gaaaaacgat aaaatacaag ttatgagaat
    25261 gatcaattca gctgattaaa cgccccatcc cttttgcggt acaacgcaag actccatttt
    25321 ttcttcgacc aatcagtaca ccgttttggt cataaacatg tttgattgtt ggatatagtt
    25381 cttttaaagg gataggatct tttaaaatta tttgcttttg tttaccagcg taataaatca
    25441 tttcctctga gactttgtct ttctggatta tatatttctt acactgcaca acggttttac
    25501 tttggccagt tgatagtttc ttcgtgattt tctctgtagc ggtacaccgt ttaccgtagt
    25561 gacaaatatc ttcgagactc aaaattcctg tatatgacat aaaataatcc acccacttac
    25621 tttaaatatt tttaataagt ttttcttgtt atttttcaaa aatagagaat tgattttcgt
    25681 tcatgacaaa caaaaaagag ggaacgcatc caaacatgcg ttccctctaa tttcagcgtt
    25741 cgtcatacgg caaagagcca cacggacatt tttataacat tatgcttagg catagattca
    25801 tcgtaatatc acgtattttt aaccttaaca cagccaaaaa aagtggtatc acttttgaat
    25861 atctgttata ctaaatccgt tgcgggtaat accgtaacac tgaaaggtaa acgcattaag
    25921 ggattaaagg ttgtcttaat gccacctgtg gacgtcgacg aaggttacca gccttccgag
    25981 tctcacaggg cctttctttt ttttgtattt tcaattgtcc aaatttatcg tccaaaagtg
    26041 cagaacgaaa attaattcat cacaagcgta gcagacaatt aaatttaaaa caactaaatt
    26101 tatacaaaaa aatgtaagcg aataatattc gtattgcctg aaaagaagta atatcaaggt
    26161 ttttagcttt tttaaaaaat gaaattacct ctcttcgcgt attattataa ctaaaaaaca
    26221 gcaacatgca aacgtttgtt ctcttttttg tgttggaaat taattattaa ctattaacac
    26281 ttatgacgta aaaagctacg tttgtgattt ttatcataat attttaactc ataaattcta
    26341 ttaaaatagc tatttctttg taacatttgt aatataatta gaataataaa aaatttttaa
    26401 ttttaatgct actccatgaa gcaacaatca atttgtcagt gaaatttcac tctaaccagt
    26461 ctttcgtaat agtttttggg aagctttcag aattcaaacg tttttcactt gatactttag
    26521 cagctattgg tttttcgata aaccaatttt ttacatagac aaaaatcagg ttctggatat
    26581 tttcaacttt ttcggttttt aatttttgat aggcttttaa cagtgtcgtg tataattctt
    26641 gttctgcatt gacataataa ctatcgagtt cttcaaaaat tattgaaata ccactttctg
    26701 tttcggcttt tttctttgca ttatgaatag tctggacagt tttttgagct tcactgtaac
    26761 ttgaagaaaa tgtttttatt aaatttaata ctcgttcagg gataaacgta gcaatgctgt
    26821 cgtttgtcat aattgtctca aaattgtcta acaacaattg atcttgtagg cggtcttttt
    26881 cagtgtctat attgtgtcta ttagtgtcta atatattatt attaagttct ggtatgattt
    26941 tcataccctc tgatttatca acgttttggc acggtatgat tttcataccc tcttgagccc
    27001 caagggattc agcgtctgat ttttcgtcga gggtgggtat gattttcata ccctctgatt
    27061 tatcaacgtt ttccctattc tctctatcaa atttttcgag taagtagata tcattttctg
    27121 agacttttaa ttctgctaga tatagtctat taggattatt tttccctaac tgtttgttaa
    27181 atcccatctg cttttgtaat aataaaccaa aggattccaa ctcttttttg attgctatcg
    27241 ctttttgttt ttggcagttt aatacgctac atagttcatt tgtcgtataa gtaaaataaa
    27301 cattaccttc ttcatcgatt tgccgtttgc tgattgcgat ctctaatctt gcttttaaaa
    27361 gcatataggc aatcttagtt gaatctttca tttttttgta ttcgtcagaa acaataaaga
    27421 cttttggaaa ttgataaaat aattcgttgt aaactctatc cgccttaaaa aaattaaaat
    27481 cagtcattgt agatcctcta ctttcacgtt gttttattta tgtaaaatta cctactcttt
    27541 tcgaaaatag caaagataaa taaaaaatgc aaacgcatta aaactgtttt ttaattaaat
    27601 actacctaaa atcagaaata aaatgaatta tgtactacct ttaaagtcat taagaactac
    27661 tttaaataaa tattatttat aattaaatac taccagaaga acgtatgaat aaaatttagt
    27721 tttaaaatta aagataatta agtactacca gtgtcaaaac aaaaaataaa ttaagaacta
    27781 ccttatgcat ataaaacata ctaccattac tagtaatata tactacctta tttcagtagt
    27841 atatattata ttcttcttat aagacaaggc tttaggctag aaaacaatag attatatact
    27901 actaaaaaat acaataagta ctaccaatgt cagatatata taataattat gtactaccat
    27961 taaaattaac ttatttaata agatactact gtgttaatct gaaaaaaatt actttaaagt
    28021 agttacatac tacctgttaa attagatact accattaata cttaaattag atactaccta
    28081 tatttatatt aagtactacc gttctaggaa taagataata aaaaaaaact accaaattgc
    28141 aattaaatac tacttaaaat gtaaaaaata agaactaccc aaattaggta gttcttaata
    28201 agagaatatc agataaattt gtacaagtca taaaaaaatt aatcttaaat ttttaaatat
    28261 atatacctgt taattataaa ctactttatt taggtagtac ataattaaca gataaaacta
    28321 catgcttgta gtttgtctag ctaatgagtt gcatatgatt tatataaagg tgtgcagcat
    28381 aacttagttg aagttaagag aatagaataa atattttttg ttttgctaat aattaaacac
    28441 tacctaaata tggtagtgtt taattatata atattcattt tgttacaaat aatcgtatta
    28501 taaaagcata acaattggga taactatttt ttagattatt taagtactac ttaaattagg
    28561 tagtacttaa attttggttt aag
//
LOCUS       DAPBBG010000036        28552 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPBBG010000036 DAPBBG010000000
VERSION     DAPBBG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.036"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..718
                     /locus_tag="RD477_001784"
     CDS             <1..718
                     /locus_tag="RD477_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDT7634141.1"
                     /translation="KIHPNVKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLM
                     EWIEPGKGDQRDLAAALANLHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRL
                     ESQISLAEETNRWNVQRQEKYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGH
                     PVFVDPAVSYGNREQDIAMSQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLA
                     HLNMFGESYGSQVDQLLENF"
     gene            734..1471
                     /locus_tag="RD477_001785"
     CDS             734..1471
                     /locus_tag="RD477_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDT7634142.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1626..2594)
                     /locus_tag="RD477_001786"
     CDS             complement(1626..2594)
                     /locus_tag="RD477_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDT7634143.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            2826..3659
                     /locus_tag="RD477_001787"
     CDS             2826..3659
                     /locus_tag="RD477_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDT7634144.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3693..4532
                     /locus_tag="RD477_001788"
     CDS             3693..4532
                     /locus_tag="RD477_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7634145.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4558..5118
                     /locus_tag="RD477_001789"
     CDS             4558..5118
                     /locus_tag="RD477_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDT7634146.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5317..7038
                     /locus_tag="RD477_001790"
     CDS             5317..7038
                     /locus_tag="RD477_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDT7634147.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7203..8345
                     /locus_tag="RD477_001791"
     CDS             7203..8345
                     /locus_tag="RD477_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7634148.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8361..9572
                     /gene="thiI"
                     /locus_tag="RD477_001792"
     CDS             8361..9572
                     /gene="thiI"
                     /locus_tag="RD477_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDT7634149.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            10145..10792
                     /locus_tag="RD477_001793"
     CDS             10145..10792
                     /locus_tag="RD477_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDT7634150.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    10921..11137
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11185..13830
                     /locus_tag="RD477_001794"
     CDS             11185..13830
                     /locus_tag="RD477_001794"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDT7634151.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            14140..15453
                     /locus_tag="RD477_001795"
     CDS             14140..15453
                     /locus_tag="RD477_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDT7634152.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            15443..16096
                     /locus_tag="RD477_001796"
     CDS             15443..16096
                     /locus_tag="RD477_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7634153.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            16151..16843
                     /gene="radC"
                     /locus_tag="RD477_001797"
     CDS             16151..16843
                     /gene="radC"
                     /locus_tag="RD477_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDT7634154.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            16993..17193
                     /locus_tag="RD477_001798"
     CDS             16993..17193
                     /locus_tag="RD477_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7634155.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(17447..19126)
                     /locus_tag="RD477_001799"
     CDS             complement(17447..19126)
                     /locus_tag="RD477_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDT7634156.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(19128..19340)
                     /locus_tag="RD477_001800"
     CDS             complement(19128..19340)
                     /locus_tag="RD477_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDT7634157.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            19733..21463
                     /gene="efrA"
                     /locus_tag="RD477_001801"
     CDS             19733..21463
                     /gene="efrA"
                     /locus_tag="RD477_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDT7634158.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            21460..23220
                     /gene="efrB"
                     /locus_tag="RD477_001802"
     CDS             21460..23220
                     /gene="efrB"
                     /locus_tag="RD477_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDT7634159.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            23315..23512
                     /locus_tag="RD477_001803"
     CDS             23315..23512
                     /locus_tag="RD477_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634160.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            23665..24150
                     /locus_tag="RD477_001804"
     CDS             23665..24150
                     /locus_tag="RD477_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDT7634161.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(24254..24847)
                     /gene="clpP"
                     /locus_tag="RD477_001805"
     CDS             complement(24254..24847)
                     /gene="clpP"
                     /locus_tag="RD477_001805"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDT7634162.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            25027..25554
                     /locus_tag="RD477_001806"
     CDS             25027..25554
                     /locus_tag="RD477_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634163.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            25645..26382
                     /locus_tag="RD477_001807"
     CDS             25645..26382
                     /locus_tag="RD477_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDT7634164.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            26386..27303
                     /gene="miaA"
                     /locus_tag="RD477_001808"
     CDS             26386..27303
                     /gene="miaA"
                     /locus_tag="RD477_001808"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDT7634165.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            27305..28534
                     /gene="hflX"
                     /locus_tag="RD477_001809"
     CDS             27305..28534
                     /gene="hflX"
                     /locus_tag="RD477_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDT7634166.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         9421 a         4908 c         6127 g         8096 t
ORIGIN      
        1 aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca gaagtagacg gattaaaaga
       61 actgtcagca ttcgtacgtg taccggatac gtatatgcta ggtgaaacaa gtgaaggcgc
      121 ttatttgtta atggaatgga tcgagccggg aaaaggggat caacgagatc ttgctgcagc
      181 tttagctaat ctccatcagc aaacagcgcc acaatttggt tttcgtaaag ataactattt
      241 gggtacactt gttcagaaaa attcttttga agaagattgg tggacttttt tctttaaaga
      301 tcggcttgaa tcccagattt ctttggcaga agaaacgaat cgatggaatg tacagcgtca
      361 agaaaagtat ctgcgattta aagaacgtgt attgaaatcg gttgaaccaa aaaagatcac
      421 tccacgcctc ttgcacggag acctgtggag tggtaatgtg ttctttgacc agcaagggca
      481 tccagttttt gtcgacccag ctgtttctta tgggaatcgg gaacaagata ttgcaatgag
      541 ccagttattc ggtggtttcc gaccggaatt tttagatgct tatcaaacta tatttccatt
      601 agaaaaggga tggaaagacc gactgccgat ttaccagtta tattatcttt tggcacattt
      661 gaatatgttt ggagaaagtt acggttccca agtagatcaa ttactagaaa atttttaaga
      721 ggagggcgtg tttttggcaa aaaaatatac caaaaagcat gaagtttcat attatgagtg
      781 tgacatcaac caaacgatga catttccttc gatgttagga gtagttatca aaacgtctga
      841 agatcaaagc gaccagttgg gaagaggcag tgaattcgtc aatagctttg gacttacttg
      901 ggtcatcacg aattattcca tggagattaa tcgtctccct agagtcggtg aagtaatctc
      961 agtaacgact caagccatgg agtacaataa gtttttctgt tatcggaact tctggatcca
     1021 tgatgaagat gggaaagaat tagtcaaaat agaatctgtt ttcgtattga tggattttgt
     1081 gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct ccttttgaga gtgagaagat
     1141 caagaaaatc aagcgtcagg aaaaaattga aaagattgat tcgggcttca tgttgccata
     1201 tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt aataatgcga tgtattttaa
     1261 ttggatcatt gacgtattag gatatgactt tttgactacg catgttccag aaaaagtaat
     1321 tattcgattt gataaagaag tagagtacgg gaatgagatc gaaagccatt ttgaacaaat
     1381 ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga atgcaagaac agctgtattg
     1441 tgaagcaaat attatatgga aaaataaata aaaaagaggt tgtgacagaa gggggcaact
     1501 tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt
     1561 agttttcgaa gaagctattt ttgtcacagc ctctcttttt aagtttagct attagataat
     1621 gatgtttagt tttggataaa cgaaatcaat tcttcacccg acaaaccata gtttacagca
     1681 atcaatagtt ttaaacgtgc tttttgacta ttaatactgt tacagaaaat aatccccatt
     1741 ttttttagtt cttttccccc gccttcataa tcataaacag gctctgcgat tccattgaaa
     1801 caacgagaaa caagtactac agggattttt tggacaagaa gcttttccaa agcagctaag
     1861 gcttgtggag ggagattccc cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta
     1921 tgggcgatgg cttccaataa gtccccttgc atcccagcat atgctttaac gatcgggata
     1981 gtcccatcta cagcggaaat gtccagtcgc tttgtttcta aaagttcctg caagaataaa
     2041 attcgattct tagttactaa gccgatggga cccaatgtag gtgttcggaa agtagcaaca
     2101 tttgtggtat gggtctttgt cacgtagcgt gcagaatgga tctcatcgtt catgaccacc
     2161 aaaaccccct tatctagcgc ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta
     2221 taaaggccgt cacttccaag ttcattactt gaacgcattg ccccagtcaa aacaattggt
     2281 aatttgttgc caatcgttgt atcaagaaaa aaagcagttt cttctaatgt atctgtaccg
     2341 tgtgtgatca ccactccgga tatacctgat gcataagcgg acaagatacg ttcttttaat
     2401 accaacatgt gagaaggcat gatgtgtggt gagggaagat tgaagatatc ttcaactaat
     2461 aattctacat gcttggggat tgtgatttct gcatctaata atgggtttgc cacatcagga
     2521 ctaacaccgc ccgtgagcat gttttctttc atggcgatcg ttccgccggt gtgtagtaca
     2581 agtatttttt tcattttaca gacttcctta cgaataattt tgcatagccg ttaaatctta
     2641 ttgtatacta tataaaagag ttttgctatc agtgaagaat aacgaatagc ctaacttatc
     2701 ataaaaaagc tgaccttatt aaaatggaaa agactcaacc ttgacctcct aatacaatgg
     2761 agtatctggt tgagtttctt ttacaagaaa gcagccaagg aaatcaatca cgaaggtggc
     2821 cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca tgacaaatta actggtaaaa
     2881 agaataacac tctttatgaa tatgcagggt ttctaccctt agtggaatta gatactgcct
     2941 attatggaat tccccgaaag tcctcagttg agaattggat aagtgaagta cctgaatcat
     3001 ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca aggagactgg aagccttatt
     3061 atcagaccga agaagagatg attgcgcatt ttttagatgt tatgtcgcca atggtagatg
     3121 ctggaaagat gttttgcttt ttagtccaat tcccctcatc attcaaatgt acgaaagaaa
     3181 atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg ttatcctgtt tctatagaat
     3241 tgcgggatta ttcttggtac agcgatgaat ttcttgaacg tacaagaaac tttatgtcta
     3301 gagccgcttt ttctttagcc atcatcgatg agccacagtt gctgaataca acgattcctt
     3361 ttgatccatt tgtcaccaat ccgaactttg ctctttttcg attccatggt aggaatgcgc
     3421 aaggatggca agcaaatgat aaagagtggc gtaaaaaaag aacgttatat cgttattcag
     3481 aaaaagaact gcaagaacta gcacatgaag tcaaagtagt tgattctaag acaaaagagg
     3541 taggtgtgat ttttaataat aattctggtg gtgatgcggc tgaaaatgca ttagcactgc
     3601 aaaaaatatt aaatatcaag ccagatggac tcaatccaac ccaaatggat ctgttttaag
     3661 ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa agctattttt tttgatattg
     3721 acggaacatt ggtcaccaat cgttcaaaag cgttggaatc aacaaaacaa gcaattgaat
     3781 atgctcggaa aaacggcatt ttatgcggtg tagcaacggg gcgcagccca gtaaagatca
     3841 aagaaatcat tgatgaattg gaattagata tgtatgtcgt gtataatggt caactggttt
     3901 ttactgcaga tcgcacgatt atcgaccacc cttttgaaca aaaagtattg gaacatattg
     3961 ttgaatttgc agatgagaac catcgacaaa tcgtttttgg tgcgagaaat cggttagatg
     4021 gatcaacgac gatgctatta ggacaatcga tttttatcaa gcgactagtc agctttttac
     4081 cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca ggtttttagc cctcaccgac
     4141 aaaaggatcg ttatgaaacg ttagagatat taaaagaacc aatttatcaa tgtatattgc
     4201 tcagtccgga gtcagaacag aaaaagttag aacatcgctt tcccgagtgt acgtttcagc
     4261 gttcaaatac gtatactgta gatatcatcc caaaaggtgg ttcaaaatta ctaggcatcc
     4321 aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat tatggcattc ggcgatcatt
     4381 acaatgatat cgagatgctg aaaggtgtag gaattggagt ggccatgggg aatgctcaga
     4441 tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa tgaagaagat gggatatacg
     4501 aaggactgaa acacttcggt ctgatttatt agtcaatgaa agaaggaaaa aatcagtatg
     4561 gaagcagaga caccttttca aaaagtagaa acattccaca aaacttttga tccaagaaga
     4621 ccagagatac ccacggcttt tacagaaaat gaagctcata cacgtgctgc gttcaaaata
     4681 gaagaacttg ttgaatttgt tgcagctact tcacctaata aagaacaact gcatgaagct
     4741 gttatgctgc tgcacgaagc attagataaa gcagaagaaa aaatacttgg taaaacacag
     4801 tggggaagtg atccgctggt tgaacaagta gatgcactga cagatatcct ttactttact
     4861 tatggttctt tttcattact aggaatagtg cctgatcgaa tattcgaaat cgtccatcag
     4921 gcaaatatgg ggaaattatt tccagatgga aaacctcatt atgaccctgt aacaaataaa
     4981 gtgttgaaac ctgaaaactg gcagatggaa tatgctccag aacctaaaat cgcggcagaa
     5041 atccaacgtc agattcaaga agcgattgct aagaagaaat ccgctgatat aggcatgtgc
     5101 acaaatgaaa aagagtgatc agagtttcac tatttttgtt atctttatac gatggaaaaa
     5161 caaaagattt tcgtttaagt tagtgaatgc atgaaaatag tatgctataa tctagaatag
     5221 atgtcttttg cacgagagac gtattatttg gatttatgcc aagcagaacg ccatctgttg
     5281 cataaaagat gcatactgaa aacggaggaa ccataaatgg gaaataatgt tatcatcgga
     5341 atcgtcattg caatcattat agtcgccatt gtcgtctttt tggttagtta cttgatgaag
     5401 aaaaaaaacc aagatcgatt ggatgcctta gaaaaacgca aggaagaatt atttgacttg
     5461 ccagtgattg aagaagtcga tgatgttaaa aaaatgcatc tggttgggca aagccaaaat
     5521 acctttcgtg aatggagtca aaaatggaat gacatatcta cttcctcgtt cgctgaacta
     5581 gaaagtcaaa ttttcgaagt ggaaaacctc aatgaaagtt ttcggttctt aaaaggaaaa
     5641 aaagcagttg ttgaagcaga agctacaatg gaccaaatgg agaaacaagt gacggaaatc
     5701 cgtgaaggtc taaaggaatt gcgggaaacg gaagaacgca actcactagc tgttcagcag
     5761 gcacttgacg tctatgaaga actgaaagaa aaagtcagag aagacgagca tgaatacgga
     5821 ccagctcttt ttgaattgca aaaacagatc aaagtgatcg aaaaagaatt tacgcagttt
     5881 gtgactttaa acacttctgg agaccctgtt gaagcccgcg aggttttgga taaagcagaa
     5941 aaacatacgt atgaagtgga agctctgatg aaaaaaattc caccgctgta tgaggagctc
     6001 cataccactt tccctgaaca acttgaagaa atcagtgata cttacgagaa attgatggaa
     6061 gaacaatatg tattcccaga agaagatctt gctgaagata tcgcaaaagt aagtcgaaga
     6121 attgaaaatt cattagccaa tcttgaaaaa acagaagttg aaacggtcga atttgaaaac
     6181 cgtgaaacag ctgatttgat tgattcgtta tatgatatct tagaacggga aatggaagct
     6241 cagagatatg taaaaactaa ccaatcaacg attgcagagt atatcaaaca tacaacaaag
     6301 aataaccgtc aattattgat tgagctggat cacacatctc aaagctatac attgaaccac
     6361 aatgaaattg gccgtgtacg aggtttccaa accgaagtgg aagaaatgga acgtcaaaat
     6421 gaacagatga ttccacagat ccgtcaacac gaaattcctt attcagaagt gcgcacgttc
     6481 tataaaacag tcttcaaagt cttagaagat atcgaaaagc aacaagtaga gatcgacgac
     6541 tcattacatg aattgagaaa aggcgaaaaa gaagcgcaag aaaagatcga tacattcgaa
     6601 ttcaaattgc gcagtttgaa acgttttgta gaaaaacaac gtttgcctgg cttgccgaat
     6661 gattacctag aattcttttt tgttgctact gaccgtattg aagaactaag tgttgtcttg
     6721 aataaaattc gagtaaacat ggaagaggtc aatcgtttag ttgctctatg tgaagaagat
     6781 ctagaattat tagataagaa gacacatgat ttagtagatg cagcagcatt aacggagcaa
     6841 atgctccaat atgcaaatcg ttatcgtcat acacacgaag aagtgagaga agcaattgat
     6901 aaaagctact atttattcaa taaagaatat cactatcaag aagcattaga cgaaatcggt
     6961 acagcattag agcgagtaga acctggtgct ttcaaacgca tcgaagattt ttattttaat
     7021 catccagact tagtataaag caaaaaagtt agtaaaccag caagagcaag acggattatt
     7081 gtcttgttct tgtctttttt tgtatatttg aaaattgaaa caagtgaaaa tttcacctgc
     7141 tcttgaaagc accttggaca accgttataa tagaaaagaa tttcaaaaaa gaggggtttc
     7201 ttatgatata ttttgataac agtgcaacaa ccccaatctt tccgcaaagc ttagatgcat
     7261 atgtaaaaac cagtcagcgg attattggga atccatcaag cttgcatgat ttaggcaatc
     7321 aagctaaccg gttgttgcag caagcaagaa agcagatcgc tgatttgatc catgtagaac
     7381 cagaagaaat ctattttact tcaggcggaa cagaaggaga taactgggta ttaaaaggaa
     7441 cgatgatcga aaaaagagaa tatgggaatc atatgatcat ttcaggagtc gaacatccgg
     7501 ccgtcagcga gacagcagaa caattaaaag aattaggaat cgaagtaagt atcgccccag
     7561 tggataaacg cggatttgta cgtgtcgacg aattgaaaga attaataaga aaagagacag
     7621 tattagtctc tgttatggct gtgaataatg aagtaggatc gattcagccc atccaggaaa
     7681 tcagtgatct tttggatgat tttcctaaaa ttcatttcca tgtagatgct gttcaggcga
     7741 ttggaaaagt accattctcg caatggttga cagaaagagt tgattttgca acattttcag
     7801 ctcataaatt ccatggccca cggggaactg gtttcattta ttggaaaaag ggtcgtcgtt
     7861 tagcaccatt actgacaggt ggagggcaag aaagaaatca gcgaagtggg acagaaaatg
     7921 ttccaggtat tgtggctatg gttaaagctt tacgtttatc attagagata aaggaacaaa
     7981 aacctgagca gacacaacgc ttaaaagtag ctctaatcaa tgcgttgaag acgtatgaga
     8041 aagtgacgat tttttcagaa ggaactgact atgcaccgca tattttatgt tttgctctaa
     8101 aaggaattcg cggcgaagtt ctcgtccatg ccttagaaga aaaacaaata tttgtttcta
     8161 ctactagtgc ctgctcctca cgtaaacata tggcaagcag tacattgcac gcaatgcatg
     8221 taccaaatga tctggctaca tctgctgttc gaatcagctt agatgaaaac aattcgcttg
     8281 cagaagtcga gcaatttatg attattttca agcagcttta taagaaattt tctgcaatca
     8341 attaaaagag gagtgttctt gtgaaataca gtgaaattat ggtccgttat ggggaacttt
     8401 cgacaaaagg aaagaatcga aaaagcttta tcatgcaact agcacaaaat gtcagacaag
     8461 ctttgaaaga ctttccagaa atcaaaatcc atgctgatcg ggaccggatg catcttttat
     8521 tgaatggcgc agacgggcag ctagttattc caaaactagc aaaaatattt gggatccaaa
     8581 atttctctcc aagtattcgt gtagaaaaag atgtcgaagt tttgaaacaa gctgttcaag
     8641 acatcatgaa agaaatttat actgggaaag aaacatttaa aattatcgct aaaagaagcg
     8701 atcaccaatt tgaattgaca agtaatgaat tgaatcaaac attaggtaat gctgtttttg
     8761 atattttccc gcatatccaa gttcaaatgc gtcaaccaga tattccatta cgtgtagaga
     8821 ttcggcgtga tggcgcttat ctatcgtatg aaacgatcaa gggagctgga ggattgcccg
     8881 taggaacaag cggaaaagga atgcttatgc tttctggggg gattgattct cctgtagcag
     8941 ggtactttgc catgaagcgg ggagtggaaa ttgaagctgt tcattttgct agtccgccgt
     9001 atacaagtga acaagcactg caaaaagcaa aagatctaac agccaaacta gcaccttatg
     9061 tgggaacgat ccattttatc gaagtgccat ttaccgagat tcaagaagaa atcaaaaagt
     9121 cagtgccaca aggatattgg atgacaatta ctcgtcgaat gatgttgcgt ttgaccgatg
     9181 aaattcgccg tattcgtcat ggtttagtca ttttgaatgg tgaatcatta ggtcaagtgg
     9241 cttcacaaac tttgcaaagt atggtggcta tcaatgaagt aacgaatacg ccaatcattc
     9301 ggcctgtagc gacaatggat aaacttgaaa tcattgaagt agcagaacag atcgatacat
     9361 ttaatctttc tatccagcct tttgaagatt gctgtacgat ttttgctcct ccacagccaa
     9421 aaacacgccc tcaactggaa aaggtccatc aatatgaaga gcgtttagca atagatgaaa
     9481 tgatccaacg agcactagct ggcttgaaga tggaagagat acaagcagaa tcgtctaatg
     9541 aaaaaactga agaattttca gaattgctat aagaggttat gaaaatagtg ttttgaccag
     9601 agtattagag aaacaaaacg aaaaaatagc tccatgtatt ttaccgtttt tgaactaatg
     9661 tcgaagatta gctgattgaa taccatttat caagaagtta tggaaaagcc ggttcttttt
     9721 tactaactga aaaataaaat tttatttaga gatcaagcta cagttgataa atgatataaa
     9781 aaagagaatt tcccatgaaa tagcgaggtt gtgacagaag tgggcagctt caagaaataa
     9841 gaaggaattt acgaaaattg cttttcaaat ttttgtgaaa tttcggctta tttttcgaag
     9901 aagttacttc tggaacaccg tgtatcagag gttgcgaaaa agctgccctg catcaagtaa
     9961 taagaacagc caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc
    10021 agatgcaagc ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa
    10081 ccttgctccc gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt
    10141 cgttatgcga gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata
    10201 tttacgctac ttgaagatgc tggatgactc aggtataagt agaatcaaat caaatgagtt
    10261 cagcgaaatc acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga
    10321 gctaggcaga agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat
    10381 ccttaatact aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc
    10441 attgaaacac aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa
    10501 tgatccagca ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt
    10561 cgcggagata gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata
    10621 ttcacaatct gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc
    10681 gcctgacaga gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga
    10741 gttgcagacg cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt
    10801 gagtaggctt attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact
    10861 aaatgaagtg gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga
    10921 agcataacgc tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg
    10981 ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag
    11041 tagagataga cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc
    11101 aatttaggtg gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt
    11161 tttagtttaa aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat
    11221 ccgcaagaag ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg
    11281 agcggagata aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga
    11341 aaacttcatt tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag
    11401 agaatgcaag gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca
    11461 caagcaaaag tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt
    11521 gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa
    11581 cagtgggcta aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat
    11641 ggattatctg aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat
    11701 cgtggtgaat atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa
    11761 gttattcata aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga
    11821 tcaggcgtag tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt
    11881 gctgttcatc cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct
    11941 ttagtcaata aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca
    12001 ggtgtagtaa aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat
    12061 gatcttccac gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa
    12121 tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc
    12181 ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt
    12241 gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa
    12301 aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat
    12361 caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg
    12421 tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg
    12481 gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt
    12541 agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat
    12601 aagcgatact tccctactag cactttagta accggttatg acatcatttt cttctgggta
    12661 agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg
    12721 attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga
    12781 atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg
    12841 aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg
    12901 aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg
    12961 accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact
    13021 cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa
    13081 gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg
    13141 agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta
    13201 gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa
    13261 gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt
    13321 gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg
    13381 attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg
    13441 ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat
    13501 atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat
    13561 ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc
    13621 aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta
    13681 aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta
    13741 gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa
    13801 cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta
    13861 gctattacta aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct
    13921 tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact
    13981 cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga
    14041 taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt
    14101 tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata
    14161 gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct
    14221 ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca
    14281 aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca
    14341 gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc
    14401 aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa
    14461 caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg
    14521 gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc
    14581 ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga
    14641 cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga
    14701 atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc
    14761 atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa
    14821 ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata
    14881 ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga
    14941 gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa
    15001 gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac
    15061 gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag
    15121 aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact
    15181 aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg
    15241 acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag
    15301 agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca
    15361 aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta
    15421 ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt
    15481 gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg
    15541 ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg
    15601 ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga
    15661 tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg
    15721 tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag
    15781 taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga
    15841 gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt
    15901 agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa
    15961 tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc
    16021 accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc
    16081 tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg
    16141 agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga
    16201 acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact
    16261 gcgtaccggt caacacccct atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt
    16321 tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat
    16381 aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac
    16441 ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc agcaattgat
    16501 tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa
    16561 tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc
    16621 tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa
    16681 tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca
    16741 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
    16801 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
    16861 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
    16921 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
    16981 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
    17041 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
    17101 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
    17161 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
    17221 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
    17281 ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa
    17341 gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt ttttcatttg
    17401 ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat tcttcatctg
    17461 gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat aagaaaggct
    17521 gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt ccattcaaac
    17581 attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct cctgattctc
    17641 gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct tttttcttga
    17701 tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga cggtcacgta
    17761 aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct ccagccacaa
    17821 aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca ttttccatga
    17881 tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt actttcaaca
    17941 tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga agcattaatt
    18001 tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt ccgtgaatca
    18061 cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt gtattaccag
    18121 gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt tgacggtgag
    18181 taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct gtacatagga
    18241 tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat gtgccgtctg
    18301 gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg cttcgtccga
    18361 aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc aaacggaaga
    18421 tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa
    18481 tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca tttgtactat
    18541 cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc aagtttgctg
    18601 gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata tttccagaag
    18661 gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg aaaaagctga
    18721 cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc tctgcatctc
    18781 gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga ggaccagcat
    18841 agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc
    18901 cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag ctgtagtcgg
    18961 gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg gcatcaatca
    19021 aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca cctaatccac
    19081 caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta aaactccact
    19141 aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag aagttgaacg
    19201 aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac atcgcttttt
    19261 gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga
    19321 taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag tgatttagct
    19381 agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt tcatatgctt
    19441 tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta tctgttcgga
    19501 tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt tattctagca
    19561 taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa tgtttatcat
    19621 cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag gctcgttgtg
    19681 ctatcatttt tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga aagtgaaact
    19741 aatgtggcat tataccatgc gttataagaa attcttgttt ttcaatttca tatgcgtatt
    19801 tggttttatt ttgattgaat taggacttcc tacgatttta gcacgtatga tcgatgtagg
    19861 aatccgcaat gatgattttg attatgtgaa gcaacaaggg atatttatga tcgttattac
    19921 tgtaatcggt attattttga atatcttgtt aggttatttt ggttcgagga ttacgactaa
    19981 tatcgtagca gatattcgag atgatttgtt cgaaaagatc caaagttatt cgcatcagga
    20041 atacgaaaat ctaggcgtat cttcattgat cactcgtaca acaaacgatg cttatcagat
    20101 catgttgttc ctgcaaaata ttttacgtat cggttttatg gcaccaatga tgttcattgt
    20161 cagtttgtac atggtcatgc gtacaagtgt gacactcagt ttgtatgtag taggtgcatt
    20221 acctttacta ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa
    20281 gcaacaaaaa aacttagata aaatcaacag tatcttgcgg gaaaatcttt caggattgcg
    20341 tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga
    20401 gaattatgct tctagttcaa aaagcttatt ccgtttgatg gctgttgcac aaccgggttt
    20461 ttatttctta tttaatatcg taatggtatt gatcatctgg agcggggcag tgcaaatcga
    20521 tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc gagtatatat tccatgcctt
    20581 gttctcattc atgttatttg ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc
    20641 tggacgtatc caagaagccc tagatgccaa accttcgatt gtagaagatc caaatggtat
    20701 atctgataca aaaactaaag ggtatatcga atttcaaaat gtaacatttg cttatccagg
    20761 ccattcgcaa gagccagtga tacgaaatgt cagttttaca gcctctccag gagagaccgt
    20821 tgcttttatt ggaagtaccg gtagcggaaa atctacgttg atccaattga tccctcgctt
    20881 ttatgatgtg agtgaaggaa agatcttgat cgatggagta gacgtgagag attatcggct
    20941 aagcaagttg cgagataaga ttgggtttat tccgcaaaaa gcgctgctat ttactggaac
    21001 gattgcagat aatttgcgat acgggaaaga agatgctacg caagaagaaa tggaacgtgc
    21061 agctgagatt gctcaagctt cagaattcat ttctcgaaaa ccagatggat ttaatgaaca
    21121 tttatcagaa ggtggttcca atttttcagg tggacaaaaa caacgattgg caatagccag
    21181 agccatcata cgccgtccgg aaatctatat ttttgatgac agcttttctg cattagatta
    21241 tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt
    21301 gatcgtagca caacgtgtgg gaacgatcat gcatgcagat aagatcatcg tattaaatga
    21361 aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta
    21421 tgatattgcc gcttctcaat tatcaaagga ggaattagca tgaaaaaaac cttttcttcc
    21481 ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga cttttctttt agtgatccta
    21541 tttacagttc tgacagtggc tttcaacgtg gctttgccat atgttacagg acttcctact
    21601 acagaaatca gccgtaatat tgccaatggc gaatcaatca atttttctta tatattcaaa
    21661 tgtctcattt ggatcacagt tgttggaata gggtattgta tttcgcaatt actttctggt
    21721 gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga catagaagaa
    21781 aaaatcaatc gtcttccggt ttcatatttc gataaaaacc agcaaggaaa tattttgtct
    21841 cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac aacaagcttt tattggtatc
    21901 gtaaatgcga tattgggaat tacgttggct gcagcaatga tgttttatat ccagccgatg
    21961 atggcgctga tctctatgat catgattcca acatcgatct ggatctcaaa acgagtgatc
    22021 aatgcttcgc agaaatattt tcaaagcatg cagaattctc ttggtgagct aaacggctac
    22081 gtccaagaaa atatgacagg ttttagtgta ttgaaagtat atggacgcga aaaagaaaca
    22141 ttggaaggat tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg tgcagcattc
    22201 atttctggct tgatgatgcc tctagttcaa ttaactgcct atgctactta tattggtatg
    22261 gctgttctag gaagctttta tgttattact ggggcgatcg ttgtcggtca actacaagca
    22321 ttcatccaat atatttggca aatcagtcag ccaatgggga acgtgacaca attgtcttca
    22381 gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga tgagccagaa
    22441 gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga ttttaggaga tgtgacattc
    22501 gatcatgtcg attttgcata tgatccgaag aaaccattaa tccaagattt gaattttgaa
    22561 gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa gacgacattg
    22621 atcaatctat tgatgcgttt ttatgacgta gacaaaggag caataaggat tgatggaatc
    22681 gacaccaaat caatgagtcg cagtgatgtg cgttcgctat ttggtatggt cttgcaagat
    22741 gcatggcttt atcaaggaac gattgcagat aatatccgct tcggaaagct tgatgcgaca
    22801 gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg
    22861 ccagatggat atgatatggc catcaattct gagggagaca atgtgtcttt aggacaaaaa
    22921 cagttgctga cgattgcccg agcagtggtc tccgatccta aaatcttgat tttagatgaa
    22981 gcaacaagtt cagtagacac ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg
    23041 atggagggac ggacaagttt cgtcatcgct caccgtttat cgacaatccg tgatgcagat
    23101 ctgattctag tgatggatca aggtcagatc attgaaaaag gaacgcatga gagtttattg
    23161 gcacaaggtg gattttatga aaaactgtat aacagccaat ttgctgagga agcagaataa
    23221 gtcatataag aataaaacag tttaaaactt attttttgtt ctctattctt gataatggta
    23281 aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa cagtttttaa atgaaaaaat
    23341 tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga agccttttga
    23401 ttcagctgat ttttttgaaa atgagtttca aatcgaacgc ttggcagcac aaatagaagt
    23461 gtacgaatta attttaaaaa aagtaataga agaaaatata gatgtacaat aaaaacaggt
    23521 aaaacgtcag ttaccagata ctgtaaatag acatctggcc tgagcaggtt ttacctgttt
    23581 tttttcttat gaaataaata tatgctatga tacctttatt caataggatt agtttctaca
    23641 aaaaatgaaa ggagaaaaat aaatatggaa cggtttatag catcaaatgc cacagtgatt
    23701 ggtgatgtga cattgtcaga agatgtgacg atctggtatc aagctgtttt acgcggggac
    23761 agtaattgga taaaaatagg acaaagaacg aatatccaag atggtacaat catccatgtc
    23821 gatcatgatg cacctgtaga tattgcggag aatgtgacgg taggacatca gtgcatgcta
    23881 catggctgta caatcgaaaa aggggcgctg atcgggatgg ggaccacgat attgaatcat
    23941 gcggttattg gtgagaatag tttgattggt gctggatcat tagtaacaga aggaaaagtt
    24001 attcctccta atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa
    24061 gaagaaatac aaaaaaacaa agaaaacatc cagcattatg tcgagatagg tcggatgcat
    24121 gcgcaaggaa aattcaagga gcaaagatag gcagtagcat ccataaaata aaaaaggatg
    24181 gtcagtttga gttgaccatc cttttttatt tggtttagat aagagagctt aaaaaagctt
    24241 cctttgaaac agattaattt aatgaagaac tgttttccat gacttcatcg atcaatccat
    24301 atgcttttgc ttgttcagct gtcatatagt tgtcacgatc tgtatctttt tcaatcactt
    24361 caagcggttg tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa
    24421 tatggcgtgc cgcaatttcg atttcagttg cttgcccttg agcgccgcca agaggttgat
    24481 ggatcatgat ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca
    24541 agaagcttcc cattgaagca gccataccca taacaattgt ttggacatct gctttgacaa
    24601 agttcattgt gtcgtaaata gccatacctg ctgtaactga accaccagga gaattgatat
    24661 aaagatagat atctttttca gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg
    24721 aattagcaag atcatctgtt acttggccgc ttaacatgat gatacggtct ttcaataaac
    24781 gtgagtagat atcgtaagct ctttcacctc gtgaagattg ttcaatgact gtaggaatta
    24841 aattcataat agagttcctc cttgatttcg tttagaaata gtgtaccata atttagtttc
    24901 taatacctaa aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa aacttaggca
    24961 aattagtcgc ataaacccgc tttatggtat gataagcaga gaaacaaaac aaggaggaac
    25021 aacagattga tagttggtat tactggcctt atcggagtgc tgacaggtgc tgtagttgtc
    25081 tatatcggca tgatactgca aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa
    25141 ttggaattga aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa gatcaacgag
    25201 atcttgcaaa aacggacaac gctgatggaa gaatatgtca gctttgatgc atttgatgat
    25261 tgttatatca cgattgacga ttttgcgtat ctccaatcat tttcagcgca aaatagtttt
    25321 tatctgccaa attatatttt agacgagcta tttaagaata tctcccatcg aaaagtgatt
    25381 ttatctccag atgaaacaat gaagattggt ggatatgctt ataaaggcgg acgcgtgatt
    25441 ttagagaatt ttacggatag tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag
    25501 actatgacgc gccagccgat tggtttcttc tcgaaagatg atgtttggag ctgatatttt
    25561 gaagcattgc cgttgcacat gagagcgtgg taatgctttc tttttttctt aatgatacaa
    25621 tagaactgaa cggagtgaag aaagatgtta caaatctatg cccatcgcgg aagcagcggc
    25681 acccacccag aaaatacgct tcctgcattt gcagaagctg tacgagtagg tgcagatggt
    25741 atcgaactag atgtccatct aagcaaagat ggttatttga tcgttatgca cgacgaagaa
    25801 gtggaccgga cgactaacgg taaagggctg attcgagaaa aaacattgga agaactcaaa
    25861 aaattgaatg cagggggcaa attcagcaaa gaatttccag cagctaaagt tcctgaattg
    25921 agagaagtgt taggactgct cctccaaaaa aattaccgag ggatgctaat gattgaaatt
    25981 aaaactgatc agtatgaata tcccggtatc gaagaaaaat tagccgaaac attaaaaagt
    26041 agagattggc cttttcaaca tgcatattgc agctttcatt tggattcttt ggaaaaaatc
    26101 gcattactgg aaccggagat acagctggac ttattgatga gtacttctga taaaaagatc
    26161 caactagcag cagaaacccc ttatatagaa ggagtacatc caaaaaatac ttggttgttc
    26221 gaacatccag tccaggcagc tgattttcca aaagcaattc gtgcatggac gattaacgat
    26281 gaagaagaaa tggccaaatg tcaagaactc aaaatagatg gaataataac tgattttccg
    26341 gagaaagctc ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga aaaagtatta
    26401 gtgatcgtcg gtccgacagc agtcggtaaa acagcgctaa gtgtggaact cgcacaacta
    26461 tttggaggag agatcatcag tggtgattca ttgcaaatct ataaaaatct ggatatcggt
    26521 acagcaaaaa tcaaagaaga agagatgaat aatgttccgc atcatttgat tgatgtgatc
    26581 gaacctactc aacgttactc agccgctgat tttcagcatt caggtcgaca attgattacc
    26641 gagattgcga accgagggca cctacctatc atagctggtg gtacgggtct ctatatccaa
    26701 tcccttctat atgattatca gttaggagcg acagaagaag cgaatcctga gattagaaaa
    26761 aagtatgaaa cgttagctga aacaattggt tcccaagcat tatggaatca gttgaaagaa
    26821 aaagacccgg cagctgctga gaaaatccac tggaacaatc aacgcaaagt gataagagct
    26881 ctggaagttt tagagacgac aggacatagt atcacggcac ctaaagaaga gccaaagcaa
    26941 ttgtatgatt acctcatgat tggattagat acgaagcgta gtattcttta tgaacgaatc
    27001 aatcaaagag tcgatttgat gctagcagaa ggtttagtag cagaagcgcg taaggtgtat
    27061 gagcttggag aagtccaggc cagtcaaggt attggatata aagaattttt cccttatttt
    27121 gaagggaaag aaacattgga agaagcaaca gaacaagtca aactacattc gagaagatac
    27181 gcgaaacgtc agttgacgtg gtttcgtaac cgtttggatg cacagtggtt tgatcttata
    27241 agtgagccaa atcaaaaaag tcagattgag caaacaatca aaaaatggct ggaggcggaa
    27301 taaaatggaa gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt
    27361 ttcagagtca atggaagagc tagttcagct gacgaatacc gcatcaggag aagtcgtatt
    27421 cacaattaca caaaagagac cacaagtgga tcggcaaacg attatcggta aagggaaatt
    27481 acaagaatta gttcaacaag cggatgcgca tgaagcggat ttgatcatct ttaatcatga
    27541 gctaactccg agacagagcc aactgattac cgatgctgtt ggtcttccgg taattgatcg
    27601 cgtgcagctt atcttagata tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca
    27661 ggttgaactt gctcagttag aatacctgct gcctcgttta gtagggcaag ggaaaacatt
    27721 atctcgtctt ggcggtggga tcggaacaag agggccaggt gaaacaaagt tagaaacaga
    27781 ccgccgacat atacggaata aaatttttgc tgttaagaaa gaattgaaag aagtagaagc
    27841 tcatcgagag cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga ttggacttat
    27901 tggctacacg aatgccggga agtcaactat cttgaatcta ttgacacaag cggatactta
    27961 ttcgaaagat caattgtttg caacgttaga tccattgact aagagatggc gttttgctga
    28021 aggatttgag attactgtaa cggataccgt cgggttcatc caagacctgc ctacgcaact
    28081 gatcgatgct ttccattcta cattagaaga aagtcaaaat atggatcttt tgctacatgt
    28141 agtcgatgct agttctccag atcgtatttt acaggagcag actgtcttaa aattgatgga
    28201 tgaactgaat atgaaagaaa tgccgatact gactgtttac aataaggcag accagatcga
    28261 cccagcgatg ttcacgccta cgctattccc aaatgtattg atttctactc agacgaaaga
    28321 aggtaagatg gcgttgatcg aagcaatcaa acgtcagctg atggaactaa tgacccctta
    28381 tacactttct gtgccaagca acgaaggaca gaaattaagc gagttgagaa gacaaacaat
    28441 ggttttggac gaagcgtaca tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa
    28501 aaccaattca aaatggttaa gaaaaaacca ataaaatttt atttttttaa ca
//
LOCUS       DAPBBG010000037        26812 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPBBG010000037 DAPBBG010000000
VERSION     DAPBBG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.037"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..411
                     /locus_tag="RD477_001810"
     CDS             <1..411
                     /locus_tag="RD477_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7634167.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="RD477_001811"
     CDS             509..697
                     /locus_tag="RD477_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDT7634168.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="RD477_001812"
     CDS             complement(755..1108)
                     /locus_tag="RD477_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634169.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="RD477_001813"
     CDS             1639..2061
                     /locus_tag="RD477_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7634170.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="RD477_001814"
     CDS             2058..2288
                     /locus_tag="RD477_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634171.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="RD477_001815"
     CDS             2786..2986
                     /locus_tag="RD477_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7634172.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="RD477_001816"
     CDS             3013..4206
                     /locus_tag="RD477_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7634173.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="RD477_001817"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="RD477_001817"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDT7634174.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="RD477_001818"
     CDS             complement(5898..6821)
                     /locus_tag="RD477_001818"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDT7634175.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="RD477_001819"
     CDS             7017..7622
                     /locus_tag="RD477_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7634176.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="RD477_001820"
     CDS             7930..8946
                     /locus_tag="RD477_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7634177.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="RD477_001821"
     CDS             9087..10388
                     /locus_tag="RD477_001821"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDT7634178.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="RD477_001822"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="RD477_001822"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDT7634179.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="RD477_001823"
     CDS             11117..11515
                     /locus_tag="RD477_001823"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDT7634180.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="RD477_001824"
     CDS             11581..12663
                     /locus_tag="RD477_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7634181.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="RD477_001825"
     CDS             12875..14437
                     /locus_tag="RD477_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634182.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="RD477_001826"
     CDS             14430..15563
                     /locus_tag="RD477_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634183.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="RD477_001827"
     CDS             15563..16519
                     /locus_tag="RD477_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634184.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="RD477_001828"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="RD477_001828"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDT7634185.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="RD477_001829"
     CDS             17808..18626
                     /locus_tag="RD477_001829"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7634186.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="RD477_001830"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="RD477_001830"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7634187.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="RD477_001831"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="RD477_001831"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDT7634188.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="RD477_001832"
     CDS             complement(20506..21087)
                     /locus_tag="RD477_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7634189.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="RD477_001833"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="RD477_001833"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDT7634190.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="RD477_001834"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="RD477_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDT7634191.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="RD477_001835"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="RD477_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDT7634192.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="RD477_001836"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="RD477_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7634193.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="RD477_001837"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="RD477_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDT7634194.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8434 a         4951 c         5684 g         7743 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc tt
//
LOCUS       DAPBBG010000038        26153 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPBBG010000038 DAPBBG010000000
VERSION     DAPBBG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.038"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            133..3660
                     /locus_tag="RD477_001838"
     CDS             133..3660
                     /locus_tag="RD477_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDT7634195.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="RD477_001839"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="RD477_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDT7634196.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="RD477_001840"
     CDS             7448..7789
                     /locus_tag="RD477_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7634197.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="RD477_001841"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="RD477_001841"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDT7634198.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="RD477_001842"
     CDS             9146..11566
                     /locus_tag="RD477_001842"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDT7634199.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD477_001843"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD477_001843"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDT7634200.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="RD477_001844"
     CDS             13000..13737
                     /locus_tag="RD477_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7634201.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="RD477_001845"
     CDS             13751..14566
                     /locus_tag="RD477_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDT7634202.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="RD477_001846"
     CDS             14579..15226
                     /locus_tag="RD477_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7634203.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="RD477_001847"
     CDS             15242..15898
                     /locus_tag="RD477_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7634204.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="RD477_001848"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="RD477_001848"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDT7634205.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="RD477_001849"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="RD477_001849"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDT7634206.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="RD477_001850"
     CDS             18240..18758
                     /locus_tag="RD477_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7634207.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="RD477_001851"
     CDS             18865..19365
                     /locus_tag="RD477_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7634208.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="RD477_001852"
     CDS             complement(19645..20565)
                     /locus_tag="RD477_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDT7634209.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="RD477_001853"
     CDS             20730..21743
                     /locus_tag="RD477_001853"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDT7634210.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="RD477_001854"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="RD477_001854"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDT7634211.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="RD477_001855"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="RD477_001855"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDT7634212.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="RD477_001856"
     CDS             complement(23383..24144)
                     /locus_tag="RD477_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7634213.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="RD477_001857"
     CDS             24333..25427
                     /locus_tag="RD477_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7634214.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>26153)
                     /locus_tag="RD477_001858"
     CDS             complement(25513..>26153)
                     /locus_tag="RD477_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDT7634215.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8644 a         4531 c         5508 g         7470 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag
    25861 ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga
    25921 taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc
    25981 ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga
    26041 gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt
    26101 catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtg
//
LOCUS       DAPBBG010000039        25961 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPBBG010000039 DAPBBG010000000
VERSION     DAPBBG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.039"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1235)
                     /locus_tag="RD477_001859"
     CDS             complement(<1..1235)
                     /locus_tag="RD477_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDT7634216.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="RD477_001860"
     CDS             complement(1228..2820)
                     /locus_tag="RD477_001860"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDT7634217.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3330..4055)
                     /locus_tag="RD477_001861"
     CDS             complement(3330..4055)
                     /locus_tag="RD477_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634218.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4209..4535)
                     /locus_tag="RD477_001862"
     CDS             complement(4209..4535)
                     /locus_tag="RD477_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634219.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4572..4937)
                     /locus_tag="RD477_001863"
     CDS             complement(4572..4937)
                     /locus_tag="RD477_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDT7634220.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4974..5189)
                     /locus_tag="RD477_001864"
     CDS             complement(4974..5189)
                     /locus_tag="RD477_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7634221.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5318..5653
                     /locus_tag="RD477_001865"
     CDS             5318..5653
                     /locus_tag="RD477_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7634222.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5729..7726)
                     /locus_tag="RD477_001866"
     CDS             complement(5729..7726)
                     /locus_tag="RD477_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634223.1"
                     /translation="METLMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(7824..8099)
                     /locus_tag="RD477_001867"
     CDS             complement(7824..8099)
                     /locus_tag="RD477_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634224.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8332..9066
                     /locus_tag="RD477_001868"
     CDS             8332..9066
                     /locus_tag="RD477_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7634225.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9105..10247
                     /locus_tag="RD477_001869"
     CDS             9105..10247
                     /locus_tag="RD477_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7634226.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(10342..11733)
                     /gene="sufB"
                     /locus_tag="RD477_001870"
     CDS             complement(10342..11733)
                     /gene="sufB"
                     /locus_tag="RD477_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDT7634227.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11738..12208)
                     /locus_tag="RD477_001871"
     CDS             complement(11738..12208)
                     /locus_tag="RD477_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDT7634228.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(12195..13430)
                     /locus_tag="RD477_001872"
     CDS             complement(12195..13430)
                     /locus_tag="RD477_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7634229.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(13427..14713)
                     /gene="sufD"
                     /locus_tag="RD477_001873"
     CDS             complement(13427..14713)
                     /gene="sufD"
                     /locus_tag="RD477_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDT7634230.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(14726..15499)
                     /gene="sufC"
                     /locus_tag="RD477_001874"
     CDS             complement(14726..15499)
                     /gene="sufC"
                     /locus_tag="RD477_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDT7634231.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(15696..16544)
                     /locus_tag="RD477_001875"
     CDS             complement(15696..16544)
                     /locus_tag="RD477_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDT7634232.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(16568..17254)
                     /locus_tag="RD477_001876"
     CDS             complement(16568..17254)
                     /locus_tag="RD477_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634233.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(17247..18281)
                     /locus_tag="RD477_001877"
     CDS             complement(17247..18281)
                     /locus_tag="RD477_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7634234.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(18344..18600)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18676..19014)
                     /locus_tag="RD477_001878"
     CDS             complement(18676..19014)
                     /locus_tag="RD477_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDT7634235.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(19016..19372)
                     /locus_tag="RD477_001879"
     CDS             complement(19016..19372)
                     /locus_tag="RD477_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDT7634236.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(19378..20565)
                     /locus_tag="RD477_001880"
     CDS             complement(19378..20565)
                     /locus_tag="RD477_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7634237.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(20756..21601)
                     /locus_tag="RD477_001881"
     CDS             complement(20756..21601)
                     /locus_tag="RD477_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7634238.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            21835..22800
                     /locus_tag="RD477_001882"
     CDS             21835..22800
                     /locus_tag="RD477_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDT7634239.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            23177..24220
                     /locus_tag="RD477_001883"
     CDS             23177..24220
                     /locus_tag="RD477_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDT7634240.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24282..24746)
                     /locus_tag="RD477_001884"
     CDS             complement(24282..24746)
                     /locus_tag="RD477_001884"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDT7634241.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            24992..25939
                     /locus_tag="RD477_001885"
     CDS             24992..25939
                     /locus_tag="RD477_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7634242.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         7452 a         5321 c         4478 g         8710 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
     2941 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
     3001 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
     3061 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
     3121 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
     3181 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
     3241 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
     3301 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
     3361 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
     3421 aaccgaagtg gaatatcttt accaactttc tctgtcactg cattactgat acctgttaga
     3481 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
     3541 gtgattcctt tatcttcatt tacaagtttc caagtaggtg gtatagctgt gtatgtttct
     3601 acaaatgaat taagtttttc attaagtgac ttctctttta tatttccatc taaaaactct
     3661 gtatatatgg cttgagctat actaaaacaa agctggcctt gttctaataa ttttacatca
     3721 ttagttagta aagctgtgtt cacgttatca atcgcttcaa gaagagtatc tttggtgtcc
     3781 ctaacagctt taggtgtgcc ttctggaatt tccctacttg atacctcgtc cattttatct
     3841 tccataaacc ccttaaaatc atatctttct cccaaatcca tagaagtttt tgagaaagct
     3901 ttcatttctg agtctatttc ttcaataata tttaacatgt ctgatacata ttgtttaggc
     3961 tgattcgttt ttaatctata tgtggtaaat atctcaattc ctccaattat caataaaatt
     4021 gccaaacaaa tccataataa ttttttcttt ttcatttttc ctcctataca ctgctgttat
     4081 aacagtgact aatgtaaaga ttttcccagc aatcatcaga ctattattta cagggaatca
     4141 aaaaaactcc ttagtaagct tacaccttta ggagttcctg tttgaacggt atgcataata
     4201 catttaaatc aatttaacat taacacctca gtatatggta tctctacata tacacggtct
     4261 ttttttggat agtactcgac atttatattg atagcacttc cattgtctaa cgtagcttta
     4321 gcccggtacg tgttatcacc attatcatga gtgatcgtca catcatcaca tgtccgattt
     4381 aagttatact gttcttgtaa aatctgagtt accaatggta cagatgactt ctctagtcca
     4441 gatgaacccg tcattcttga aaaaattata cctataatag ccaatacaac gatacctata
     4501 atgacaaact tcttattcat atttacttta ttcaactttt tatctcctat ctccaaaatg
     4561 attttctctc atcagtaact cattgacatc cccaatagta caccaaagat ggctccagct
     4621 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tctctcgata taagtaaccc
     4681 ataataacag caacagcacc aaataccaca ggaataaatg ctaatgatac taccatagaa
     4741 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
     4801 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
     4861 gtttcttgta atgctccgca aaactggcaa tacttacttt catttgttat ttctttacca
     4921 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagctaactg
     4981 tttactttat taaatacatc agaaaacagc tcatttattg gtacacttaa tatctcagaa
     5041 agcattttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
     5101 tgctgtgtaa cattcatttt tttggctaac tcagtttggg aaagtccttt ttcttttctg
     5161 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
     5221 aatatgatgt atttcaactc ataaaatata taaatacttc aaatgtcatt acaaatacaa
     5281 caacttgttg tactctactg agaagggggg tgtattaatg aaacagaagc aatatgcaaa
     5341 ggtcattaga gaaaagcgaa aagcactcgg gtggacacaa aaagaattag ctaaaaaaat
     5401 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagagc ctaacctcga
     5461 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     5521 tgtcgtagat tatgaggaag agtttttggc tctctatcgt tctcttagca ctgaagatgc
     5581 agttcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     5641 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     5701 ataagattaa gagctatgga taacctgtct agtttaatac aattgggtct tttctcttcc
     5761 ttactctccg tccatcatct cttgttacac ttgtaaattg atacagagga actatcccag
     5821 ctcctgataa tatcaattct tttattgttt catctatccc tgcccctttc gctaaatata
     5881 ttaaaaaact actacttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     5941 caagagtgaa tccttgttta acaaagtagc ctctgttttt tattaaagca tttttcacgc
     6001 tgttatcccc aacattacgt aggcgttcac aaacagtgtt aataagttct ttctctgtca
     6061 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     6121 attgtttttc cttgctgagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     6181 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaattt
     6241 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     6301 cgtctaatcg atagacattg cttctcttat gattaaagtt tcctaacgct ttctcttcct
     6361 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     6421 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     6481 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     6541 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     6601 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     6661 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     6721 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     6781 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     6841 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     6901 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     6961 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     7021 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     7081 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     7141 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     7201 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     7261 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     7321 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     7381 tatgaactaa attgatagat aaaacctttt ctacgcttaa gtatctacta tcatctttac
     7441 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     7501 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     7561 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     7621 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     7681 tttcttgatc tacataaatt ggaatattaa tcattaatgt ttccatttgt gtttcctcct
     7741 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     7801 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     7861 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     7921 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     7981 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     8041 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
     8101 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
     8161 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
     8221 catcgacata cacataatac ctcgataaaa aaggggtgtc aacatctttg taaaatttat
     8281 ttacaacacc cctttttttg aggtatacta attttaggag gtgtggtcaa tatgataaaa
     8341 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
     8401 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
     8461 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
     8521 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
     8581 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
     8641 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
     8701 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
     8761 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
     8821 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
     8881 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
     8941 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
     9001 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
     9061 atctagtggt aagtactcct tgtaaactaa gaggagatga tttaatggca aaaatatcaa
     9121 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
     9181 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
     9241 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
     9301 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
     9361 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
     9421 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
     9481 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
     9541 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
     9601 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
     9661 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
     9721 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
     9781 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
     9841 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
     9901 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
     9961 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    10021 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    10081 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    10141 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    10201 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    10261 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg
    10321 gttctagctc ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg
    10381 attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat
    10441 gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg
    10501 ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat
    10561 ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc
    10621 gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac
    10681 ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt
    10741 gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag
    10801 catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt
    10861 tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt
    10921 ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc
    10981 atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg
    11041 tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg
    11101 ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac
    11161 tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc
    11221 tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg
    11281 gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc
    11341 tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc
    11401 tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc
    11461 ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc
    11521 gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga
    11581 tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat
    11641 cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc
    11701 gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat
    11761 gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg
    11821 tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta
    11881 attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat
    11941 gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg
    12001 tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat
    12061 cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg
    12121 agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt
    12181 ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct
    12241 tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc
    12301 gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct
    12361 accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat
    12421 gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt
    12481 gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc
    12541 ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt
    12601 ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca
    12661 aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta
    12721 ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa
    12781 tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact
    12841 acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca
    12901 cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc
    12961 gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca
    13021 cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag
    13081 agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc
    13141 aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac
    13201 gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg
    13261 acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact
    13321 tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg
    13381 tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact
    13441 tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga
    13501 cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac
    13561 tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag
    13621 ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa
    13681 gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc
    13741 cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc
    13801 cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg
    13861 agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa
    13921 agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc
    13981 gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg
    14041 ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg
    14101 attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt
    14161 catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga
    14221 ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc
    14281 tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt
    14341 tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa
    14401 aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt
    14461 gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac
    14521 tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga
    14581 tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc
    14641 aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt
    14701 ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt
    14761 ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg
    14821 tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata
    14881 atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc
    14941 ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg
    15001 caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag
    15061 aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt
    15121 tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg
    15181 cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt
    15241 gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt
    15301 tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg
    15361 cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt
    15421 tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt
    15481 ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta
    15541 gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat
    15601 tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat
    15661 tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt
    15721 ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact
    15781 tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc
    15841 acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg
    15901 ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa
    15961 gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata
    16021 tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct
    16081 tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca
    16141 ccatctttta attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg
    16201 tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa
    16261 ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat
    16321 acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc
    16381 acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg
    16441 tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca
    16501 gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta catcccccta
    16561 tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa
    16621 atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt
    16681 agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag
    16741 taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt
    16801 caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt
    16861 ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg
    16921 ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg
    16981 ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat
    17041 tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa
    17101 tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta
    17161 atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa
    17221 aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct
    17281 tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca
    17341 gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca
    17401 tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta
    17461 aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct
    17521 gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc
    17581 ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca
    17641 gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc
    17701 aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg
    17761 tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt
    17821 gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt
    17881 ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt
    17941 acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg
    18001 ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt
    18061 aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca
    18121 ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata
    18181 tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca
    18241 ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct
    18301 tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt
    18361 gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac
    18421 ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt
    18481 gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt
    18541 cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc
    18601 gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca
    18661 agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa
    18721 gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc
    18781 attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc
    18841 ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc
    18901 aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt
    18961 tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata
    19021 tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta
    19081 agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag
    19141 ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt
    19201 tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct
    19261 tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg tctaaccacg
    19321 ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta
    19381 cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat
    19441 accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca atgggatccc
    19501 tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat
    19561 catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg
    19621 aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac cgataaagcc
    19681 gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca catagatatt
    19741 gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc
    19801 cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc
    19861 aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt cagtcgtaac
    19921 caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg
    19981 ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt
    20041 tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt
    20101 cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt
    20161 tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt
    20221 aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata
    20281 gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag gagtcagttt
    20341 ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata
    20401 ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag
    20461 tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc
    20521 ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc
    20581 ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa gtctttccat
    20641 agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa
    20701 agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc
    20761 agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt
    20821 cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaatatc
    20881 gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg
    20941 agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct
    21001 tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt
    21061 ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt
    21121 ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat
    21181 cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt tcgatctcaa
    21241 aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa agatagacag
    21301 caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca ttgagcaatt
    21361 tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg
    21421 cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt
    21481 cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg
    21541 ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat aacttaaaca
    21601 tggcaccact tccataactt tttatgataa ttactataaa aactatttga tttaaatata
    21661 caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt
    21721 agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat
    21781 gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag
    21841 aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt
    21901 ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct
    21961 atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa
    22021 acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt
    22081 atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg
    22141 tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa
    22201 gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt tggatttggg
    22261 gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt cttttggatt
    22321 tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga
    22381 aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc
    22441 ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc
    22501 gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt
    22561 cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata
    22621 tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta
    22681 aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta
    22741 cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa
    22801 taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac
    22861 aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg caagcttttg
    22921 aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata agaaggaatt
    22981 tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag aagctacttc
    23041 tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga cctcttgttc
    23101 ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt atttattgac
    23161 gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa aggattcagc
    23221 ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct tcagcaggct
    23281 tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat tgggtatttt
    23341 attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag
    23401 caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc aaacgattgc
    23461 tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg
    23521 tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga tgatggctgg
    23581 aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca tccatgcgaa
    23641 agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg tgatgatgtt
    23701 gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa aaagtgcact
    23761 tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct tacttgacaa
    23821 ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc tcgttgttgt
    23881 tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg aaacgccaac
    23941 tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt ttgtgatctt
    24001 ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg cacattttat
    24061 cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag actttgcaaa
    24121 attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc ttctccaaac
    24181 agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg aaaaaacagt
    24241 tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg cagatatcct
    24301 tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc catatatcct
    24361 gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg caatggattg
    24421 gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc aacggctact
    24481 tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca gacattttgc
    24541 tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct ttttcccgca
    24601 aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc tgattcgctt
    24661 tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc taaccataaa
    24721 aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac tggaacttcc
    24781 tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta aaagtttgat
    24841 aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac ctgttctttt
    24901 acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa tgtaaaccat
    24961 atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg tcccatgttt
    25021 taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca aaggtgccca
    25081 tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa
    25141 gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa attttgggaa
    25201 agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga ccgtgatgga
    25261 tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga tcactacttc
    25321 tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag
    25381 tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag aaatggtcga
    25441 tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc tagaattaac
    25501 agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa cagaatatat
    25561 ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct ggaaattatt
    25621 caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt
    25681 tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta ttttgcgggc
    25741 attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct tcctgttcat
    25801 tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga aaatcttctt
    25861 agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga agccaaatga
    25921 attgaagcct caccaataat atacgtcaaa tgaagtaaac a
//
LOCUS       DAPBBG010000040        24245 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPBBG010000040 DAPBBG010000000
VERSION     DAPBBG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.040"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="RD477_001886"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="RD477_001886"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDT7634243.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="RD477_001887"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="RD477_001887"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDT7634244.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="RD477_001888"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="RD477_001888"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDT7634245.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="RD477_001889"
     CDS             complement(3376..4683)
                     /locus_tag="RD477_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDT7634246.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="RD477_001890"
     CDS             complement(4686..5267)
                     /locus_tag="RD477_001890"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDT7634247.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="RD477_001891"
     CDS             complement(5839..7131)
                     /locus_tag="RD477_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7634248.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="RD477_001892"
     CDS             complement(7128..7751)
                     /locus_tag="RD477_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634249.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="RD477_001893"
     CDS             complement(7754..8590)
                     /locus_tag="RD477_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7634250.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="RD477_001894"
     CDS             complement(8607..9494)
                     /locus_tag="RD477_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7634251.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="RD477_001895"
     CDS             complement(9510..10793)
                     /locus_tag="RD477_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7634252.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="RD477_001896"
     CDS             complement(11476..12159)
                     /locus_tag="RD477_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDT7634253.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="RD477_001897"
     CDS             complement(12177..12794)
                     /locus_tag="RD477_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7634254.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="RD477_001898"
     CDS             complement(12804..13628)
                     /locus_tag="RD477_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDT7634255.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="RD477_001899"
     CDS             complement(13621..14181)
                     /locus_tag="RD477_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634256.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="RD477_001900"
     CDS             14355..15311
                     /locus_tag="RD477_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7634257.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="RD477_001901"
     CDS             complement(15616..16572)
                     /locus_tag="RD477_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDT7634258.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="RD477_001902"
     CDS             16676..17278
                     /locus_tag="RD477_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7634259.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="RD477_001903"
     CDS             complement(17366..18865)
                     /locus_tag="RD477_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDT7634260.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="RD477_001904"
     CDS             complement(18881..19405)
                     /locus_tag="RD477_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDT7634261.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            complement(19491..19676)
                     /locus_tag="RD477_001905"
     CDS             complement(19491..19676)
                     /locus_tag="RD477_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634262.1"
                     /translation="MAETIKETNVEKQTTRMIVFWSFNGFFLKKGNIGYLERIYCENF
                     NMKTKFNKLSTINTTFL"
     gene            19702..20451
                     /locus_tag="RD477_001906"
     CDS             19702..20451
                     /locus_tag="RD477_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDT7634263.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20442..21227
                     /locus_tag="RD477_001907"
     CDS             20442..21227
                     /locus_tag="RD477_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDT7634264.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21196..21912
                     /locus_tag="RD477_001908"
     CDS             21196..21912
                     /locus_tag="RD477_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7634265.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22014..23048)
                     /locus_tag="RD477_001909"
     CDS             complement(22014..23048)
                     /locus_tag="RD477_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDT7634266.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23052..24110)
                     /locus_tag="RD477_001910"
     CDS             complement(23052..24110)
                     /locus_tag="RD477_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDT7634267.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7183 a         5222 c         4223 g         7617 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcag ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat tttgttttca tgttaaaatt ttcacaataa
    19561 attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac
    19621 acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg
    19681 atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt
    19741 gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca
    19801 catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt
    19861 gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct
    19921 ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa
    19981 gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt
    20041 attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga
    20101 caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt
    20161 tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa
    20221 cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct
    20281 tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag
    20341 cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta
    20401 aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag
    20461 aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa
    20521 ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat
    20581 atataaactc catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg
    20641 gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa
    20701 attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt
    20761 ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg
    20821 attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag
    20881 ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg
    20941 tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg
    21001 acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga
    21061 agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg
    21121 gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa
    21181 aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca
    21241 gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga
    21301 aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat
    21361 gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat
    21421 gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc
    21481 cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg
    21541 atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa
    21601 ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat
    21661 ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa
    21721 gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc
    21781 aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg
    21841 gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca
    21901 gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca
    21961 actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt
    22021 aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca
    22081 cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct
    22141 acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat agcagggtct
    22201 ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca
    22261 tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct
    22321 tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat
    22381 ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata
    22441 tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct
    22501 gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc
    22561 gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta
    22621 aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta
    22681 tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac
    22741 gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg
    22801 actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg
    22861 gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg
    22921 atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat
    22981 ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct catactttct
    23041 tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat
    23101 acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt
    23161 gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc
    23221 atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa
    23281 actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac
    23341 catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt
    23401 gctagccacg ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac
    23461 ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta
    23521 actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg
    23581 ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt caagccactc
    23641 atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa
    23701 tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta
    23761 gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa
    23821 aaaggttttg aaactagaat aagaataata taaataacta atccccaaat caagacattg
    23881 attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct
    23941 ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc
    24001 cccttttttc tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg
    24061 acaatcgaca tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat ctttctcctc
    24121 ctaattatgt tcatttattc acaataaaaa aagcttttat tttgctaata cgtattaaca
    24181 tttttaattt tatctttaca ttgttactta ttgaacaata ggaaattaaa gaaacttttg
    24241 aaact
//
LOCUS       DAPBBG010000041        23828 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPBBG010000041 DAPBBG010000000
VERSION     DAPBBG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.041"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(53..787)
                     /locus_tag="RD477_001911"
     CDS             complement(53..787)
                     /locus_tag="RD477_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDT7634268.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="RD477_001912"
     CDS             1114..1524
                     /locus_tag="RD477_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDT7634269.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="RD477_001913"
     CDS             1517..2218
                     /locus_tag="RD477_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDT7634270.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="RD477_001914"
     CDS             2218..3804
                     /locus_tag="RD477_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634271.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="RD477_001915"
     CDS             3823..4044
                     /locus_tag="RD477_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDT7634272.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="RD477_001916"
     CDS             4041..4802
                     /locus_tag="RD477_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDT7634273.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="RD477_001917"
     CDS             5182..5691
                     /locus_tag="RD477_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDT7634274.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="RD477_001918"
     CDS             complement(5766..6305)
                     /locus_tag="RD477_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDT7634275.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="RD477_001919"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="RD477_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDT7634276.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="RD477_001920"
     CDS             7318..8169
                     /locus_tag="RD477_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDT7634277.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="RD477_001921"
     CDS             8197..8832
                     /locus_tag="RD477_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDT7634278.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="RD477_001922"
     CDS             8852..9625
                     /locus_tag="RD477_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7634279.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="RD477_001923"
     CDS             10033..10830
                     /locus_tag="RD477_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDT7634280.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="RD477_001924"
     CDS             10808..11221
                     /locus_tag="RD477_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7634281.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="RD477_001925"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="RD477_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDT7634282.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="RD477_001926"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="RD477_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDT7634283.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="RD477_001927"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="RD477_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDT7634284.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="RD477_001928"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="RD477_001928"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDT7634285.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="RD477_001929"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="RD477_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDT7634286.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="RD477_001930"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="RD477_001930"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDT7634287.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="RD477_001931"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="RD477_001931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDT7634288.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="RD477_001932"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="RD477_001932"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDT7634289.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="RD477_001933"
     CDS             21905..22483
                     /locus_tag="RD477_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7634290.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="RD477_001934"
     CDS             22535..23017
                     /locus_tag="RD477_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDT7634291.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="RD477_001935"
     CDS             23134..23508
                     /locus_tag="RD477_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634292.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DAPBBG010000042        23753 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPBBG010000042 DAPBBG010000000
VERSION     DAPBBG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.042"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(90..395)
                     /locus_tag="RD477_001936"
     CDS             complement(90..395)
                     /locus_tag="RD477_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDT7634293.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(420..851)
                     /gene="ruvX"
                     /locus_tag="RD477_001937"
     CDS             complement(420..851)
                     /gene="ruvX"
                     /locus_tag="RD477_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDT7634294.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(848..1114)
                     /locus_tag="RD477_001938"
     CDS             complement(848..1114)
                     /locus_tag="RD477_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDT7634295.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1369..2094)
                     /locus_tag="RD477_001939"
     CDS             complement(1369..2094)
                     /locus_tag="RD477_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDT7634296.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2455..2525)
                     /locus_tag="RD477_001940"
     tRNA            complement(2455..2525)
                     /locus_tag="RD477_001940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2491..2493),aa:Gly,seq:ccc)
     gene            2642..3394
                     /locus_tag="RD477_001941"
     CDS             2642..3394
                     /locus_tag="RD477_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDT7634297.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3410..3889
                     /locus_tag="RD477_001942"
     CDS             3410..3889
                     /locus_tag="RD477_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDT7634298.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4140..5720)
                     /locus_tag="RD477_001943"
     CDS             complement(4140..5720)
                     /locus_tag="RD477_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDT7634299.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5713..6456)
                     /locus_tag="RD477_001944"
     CDS             complement(5713..6456)
                     /locus_tag="RD477_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634300.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6632..7408)
                     /locus_tag="RD477_001945"
     CDS             complement(6632..7408)
                     /locus_tag="RD477_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634301.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7656..8492)
                     /locus_tag="RD477_001946"
     CDS             complement(7656..8492)
                     /locus_tag="RD477_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDT7634302.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8503..9432)
                     /locus_tag="RD477_001947"
     CDS             complement(8503..9432)
                     /locus_tag="RD477_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDT7634303.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9624..9977
                     /locus_tag="RD477_001948"
     CDS             9624..9977
                     /locus_tag="RD477_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDT7634304.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10051..10707)
                     /locus_tag="RD477_001949"
     CDS             complement(10051..10707)
                     /locus_tag="RD477_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDT7634305.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10933..12867
                     /locus_tag="RD477_001950"
     CDS             10933..12867
                     /locus_tag="RD477_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7634306.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12953..14176)
                     /locus_tag="RD477_001951"
     CDS             complement(12953..14176)
                     /locus_tag="RD477_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7634307.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14170..16068)
                     /locus_tag="RD477_001952"
     CDS             complement(14170..16068)
                     /locus_tag="RD477_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDT7634308.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16062..17180)
                     /locus_tag="RD477_001953"
     CDS             complement(16062..17180)
                     /locus_tag="RD477_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7634309.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17263..18096)
                     /locus_tag="RD477_001954"
     CDS             complement(17263..18096)
                     /locus_tag="RD477_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7634310.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18089..19006)
                     /locus_tag="RD477_001955"
     CDS             complement(18089..19006)
                     /locus_tag="RD477_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7634311.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19233..20357)
                     /locus_tag="RD477_001956"
     CDS             complement(19233..20357)
                     /locus_tag="RD477_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7634312.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20540..21448
                     /locus_tag="RD477_001957"
     CDS             20540..21448
                     /locus_tag="RD477_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7634313.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21589..23202
                     /locus_tag="RD477_001958"
     CDS             21589..23202
                     /locus_tag="RD477_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7634314.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7409 a         4646 c         3963 g         7735 t
ORIGIN      
        1 tctagcggaa cgtattcaat ctcgtattca aaacaaaaca caaaaaagct gaacccttat
       61 ctaccaaggg ttcagccatc attttatgtc tattcttctt cttcagccat gaatgtattg
      121 aatacttctt caatcatgtc ccattctgcg tctgtttcga tttgttctaa ctcgccttct
      181 gttccatctt cgttttcgat gtatgcataa gcttgtaatt ctacatcttc atcttctgaa
      241 acaccagcag gatataaaag aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt
      301 agtaggattt catataatgt ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt
      361 ccttcatggt cgtgggaatg gttatgttct gtcataagat tacctctttt cttttattat
      421 taaaataatt gcttacctga gccgtctaaa taattttgta agatcatcac agctgctaat
      481 ttatcgatga ctttttttcg tttggctctt gaagtatttg cctgctctac tagcatacgt
      541 tctgcctgaa cagtggttaa tcgttcatct tgataatcaa ctggcaagga gaatagttct
      601 ttgatctttt ctccgtatga catggaagct tctgcccttg gtccgatgga attgttcata
      661 tttttaggta atccaactac aaaccggtct acttcatatt gtgctactaa ttccttcaac
      721 cgatcaaagc caaactcttc ttctgcttca ttgatccgaa tgatctctac tccttgagct
      781 gtccagccaa aggggtcact gatagcaaca cctactgtct tagaaccgac atccaagccc
      841 attactctca taaattcact ccatgatttg agaggtagtc cttgacaatt acttccatga
      901 tctcatcgcg ttcgtgacgg cgaatcaaat ttcgagcatc acgataacga ggaatgtagg
      961 ccgggtctcc agaaagcaag tagccgacaa tctgattgat tggattgtag cctttttctt
     1021 ccaaagcccg ataaacaatc gctaatgttt cgctcacttc ttttttgcga ctatcgtcaa
     1081 aaccaaaacg tacggtttca tctgtaaaac ccatgattct acacctcttt cccgcaaggt
     1141 aattccagct taccttctac aaccttattt tactcgaaga aagggaaaac tacaaacaaa
     1201 tattccaaac ttccgaataa attaagaaac actatttgta tgtttttttc cttttttctg
     1261 tagctttatc aaaagtactc ttttcataaa gtatacgcta tatttctttg aatgtatgtt
     1321 tttacacaat tttttcataa tattcataaa aaattcgaat agctgctact aattttttgt
     1381 ttttgttgct tgtcctgtct cataattgat cgtgtagcca tcttgaacat tgtagataaa
     1441 gacattgaat tcaatttttt tgtcttcaat gctttctgct tccaattgca ctcccctggc
     1501 taccagttca ttcccctcaa aatgaggagt gacacggtag cgtacatgat gatttgtttc
     1561 tttgatatat gccgcaatat cattttcgtg agcaagcatg tatggagcat tcaacgaacg
     1621 tgtccctgtc atcaagtttt taatgttgtt attttgacca gtcagctgga aaccaattaa
     1681 atggctgcga ttatataacc attgcccgtc acttaatttc ttttgtttcc aacctgtcgg
     1741 gtcaatatac aaaggctctc tcttttcagt agggaacaaa tcttttccaa gcatagcatt
     1801 ggcttgtcct actctgttca atttatcaat atttgaatag ctttcccatg ttcctttgga
     1861 aaggctaaga tcctcttttg taaattccgg ctgattccca ttgatttcta tttcttgatg
     1921 ttttccatca tattctggca catccgctac ttgagaagct tgtggtgtgg aatcaaaaat
     1981 agttgtaatc tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac cgaatgcccc
     2041 tgcaatcaca gcagcgacga tccccacgat cgccttggtt gtttttttgc tcatttcttt
     2101 actcctttac taacagctcc ctaaatctta acatatgttc ccctataaat aaatactaga
     2161 aagaaataga aacagcaaaa aataaaacct gttttaagat ggcttaaaaa tacaaaaaag
     2221 agacggattc ctcgtctcta atcagtcacc actctatctg taaggtattt attattattc
     2281 aaaaattttc ttcatactca agacaataca ctgatttcac aaccgcttgg taaacggtgc
     2341 gcaaaagctt gcatctgcgg taataagtcc atccaagtta aaaacataag aagttgtttt
     2401 tgcttggctg ttcttattac ttgttgcaga gcagcttttt cacaacctat tttaagcggg
     2461 tgacgggaat cgaacccgcg acatcagctt gggaagctgg gattttacca ctaaactaca
     2521 cccgcaatac aaaaacagta tacgcctctt ttattccttc ctcaagtttt tttattcttt
     2581 acgcttctgg tatgttttgt agtaaaataa aactaatcaa tcaattattg gagaagatgc
     2641 tatgacggga aaagaattag atctgttgct ggacacttgt ttactcgctg ggaaaatcat
     2701 gatggaaagc aatgcggaga tgtatcgggt agaagatacg atgagtcgaa ttgctttagc
     2761 ttctggaaat ttccgtttgg tcagctatgt cactcaaacc ggtctcttta ttggtcttga
     2821 tcgtacctcg accatccgaa tggaacaaat taccaaccgt tcgattaatt tagaaaaagt
     2881 agtaaaagta aatgatcttt cacgtaaata tgttgcttca gagctaacat tagaagaact
     2941 ttactgcgaa ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct ggttgcaaat
     3001 cgttagcgcg gcagtgatca gtggaacaat tatggtgtta tttggcggaa agatgaccga
     3061 tttacctgtg actttagtca ttggaggatt gggatacgca gcttatctat atagtttgaa
     3121 attttttaga atcaaatttt tatcggaatt tttatcttct ttattgattg gcatagccgc
     3181 tatctacagc gtacgttttg gcttaggaag caatcaggat ctaatcatta tcggatgtgt
     3241 gatgccttta gttcctggag ttcaaattac gaacgctatc cgtgatttgc tagctggaca
     3301 ttatgtctca ggagtttctc gcggaacaga agcgatgatg actgctacga tgatcggttt
     3361 tgcgattgct tttattttcc agctttttta ttaggaaagg aacacaagta tggggacgat
     3421 atttattcag ttttctttta gttttttagc gactgcggca tttgccgtta ttacaaatgt
     3481 acctaggcgt tcattgattt gttgtggtct ttctggtgct tttggctgga tgatctactg
     3541 gatctgtgtt caaataggcg gatctgctgc ttttggttca ttgcttggtt ctttaggagt
     3601 agcattcatc agcgatcttt tctctaaacg tttgaaaatg cccgtaacga ttttcaatat
     3661 tcccgggatg gttcctctag tccctggtgg tttagcttat caagcggttc gaaatctcgt
     3721 tactgaatcc tatcgagaag cggctcatta tgcggtccaa gcaattatga ttgctggagc
     3781 aatcgcttta ggtcttgtat tatcagaagt ctttaaccat aacatccgta atttcaaagt
     3841 aaagcgtgac gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa cgaagtgtaa
     3901 caaagaaaag gttgtgaaag agacgttcag ccttgagcga tgacaaaata tctcacaaac
     3961 attgcgacaa gtaaccgcag gagcaataag aagtaattat gagtattaaa aaaagttttg
     4021 taaaaatagc ttctcacatt tttacataaa attgacttaa tactgagaaa ctgacttttg
     4081 aacaccctct atctgtaaaa aaggggctgc gacatgattt ttgtcacggc ccctcttctt
     4141 tattcaaatt ttttccaaaa cgttcgttga taataaacga tccatagagc ggaaagtatt
     4201 agaactaaac cgatcacagg aatattcact aaccaaaatg gataaaacaa agccaacatt
     4261 ccgtataaga cgataagaat aatactgatg atcaatgaac ccatcatagt tgcgactaaa
     4321 cccaaacttc ctgaacctct gttaaataat tggttaatat tggtccagtt cagcagcagc
     4381 aaccgataat ctctagcgaa ataacgtagt gacaataagt agcagccgaa taaagaacca
     4441 aatagcaagc tagggataaa aaacaaaggc agctgaaaag acagtccgct taataatgcg
     4501 atcccaccag ttaaaaaact ctgcaatatc catccgaatc tgaacttttc ttttaaatat
     4561 tggttcatag agattggcaa agaacggata aacagaaaat tttcttgatc caaagaaata
     4621 agattgctga taaaagaagt ctgattaact gttaagaaag ccaatgcgat ccctaccagg
     4681 aaaacaacac cgattaactt attatctaaa tcagttaatt ttatttcgcc tcctagggca
     4741 aaagcaagaa taaaaatgat tggagttaac aaggaagaag aaagaacttg cataatcaaa
     4801 ttaggttctt ttactaattg agcattgtaa ctaaataaaa gctgatgcac attttggttc
     4861 tttttatggg ttctttttat tttgaactca ctgtttgaat cagtcagttg ctctgtaagc
     4921 tttggtacta tcagctgttt tacagcaagg aaaaatccca gagccgtgat agctagtatt
     4981 ccccaaccca ataaaccttg ttgagaaagt ggctgactgg cagcatcata gaatggcaat
     5041 aatatcgaaa tcggatgccg atctgttaac tcattttcaa tagtccccgt ttgatgattc
     5101 atccacatga ttccagcaaa tgcgatcagc atcgagctgc caagcaagat actagaaact
     5161 gtttttctat gttttttaaa cactgctgtt cgagccaaac caaaaacaat caaactagcc
     5221 agacaaaaaa gaataatcaa aaatagagaa aacaaaagga ttgagataat gatagtaagc
     5281 aaaatcccta gatcagctcg ccatccggtt aaaataaaca tgactagaag cggaaaaaca
     5341 aaaggaatga tggtcaaagc aactaccaaa atctttgctg aaaaaacctg ttcttgtcca
     5401 aaaggcagag gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt ataaatgact
     5461 gatatacttt gagttaaacc taacatacca aaaagtgcca cgtaataagt aaagaaacct
     5521 ggcaatttaa caaagttcat agcaaacata gcaaatccgt aaatcagtaa aaaaatcagc
     5581 cctgaaaaaa gattttgaga caaaagatag atagttaatc gctttccctt ttttcctttc
     5641 tctcttgctt tattcgtgag ctgtggatta acatataaaa gattaactcg aattaattct
     5701 aataattgtc gcttatacat acggtccatc tcctgtcgct tgccttccag ccattttcaa
     5761 ataaatcttt tctagcgttt catcaggtga gccattcaat aattcactaa ctgccccctg
     5821 ataaatcaat tcacctttct tcaaaatagc cagtttatcg cataactgtt gtgcggtatc
     5881 caatacatgt gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct tcatcatttc
     5941 tttcaaatca aatgctgctt gaggatcaag tccttgcaaa ggttcatcca atacccaaat
     6001 atctggatct ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc catgtgaaaa
     6061 acttgcgatt gtttcatttt gatgactata catatcaaaa agcgctgtca attctgctaa
     6121 acgcttttct tttttttgtg tgtctaattc ataggcagct gaaatcaagt cccaatattc
     6181 tccagccgtc aattgtaaaa aaatatctgg ggaatcaggc acataaccga ttttttgttt
     6241 gatactcaat cgattttccg tcaatttcat tccatctact aaaatcgttc ccgatgatgg
     6301 ttcaataata ctaactaaac ttttaatcgt agttgatttt ccggcaccat tatgtcctaa
     6361 aaatccaaaa atttcccctt gtcttatcga gagagacaag tcttttaatg cttgtttttt
     6421 cccgtaactt ttacttacat tgatgaattc aatcatttct gtactccttt tgtttgttat
     6481 tactttttat tgtacccttc gttattggtg gagtaaatgt caaaaagagt gcataacaaa
     6541 aaaggataga aacgactagg ctgctacttg ggttgttctc ttctacagga aaacactctt
     6601 ctaacaggta agatatgagt ctttgttttc tttaaaatga ttcattttct atcttttctt
     6661 gcataaaatt ctttagttta tcaatacttc tctttcccat ctctagttgt tctccatttg
     6721 caaatgttac tatttcgttt actctgctaa tagatgtaat gttttcagta ttaatgatat
     6781 aagcttttaa atctttacat aaatactcat attcgagtga ttttttatat tttttaattg
     6841 tgccgtttac gagaatctct tcatctttca atactaggcc tattttattt ttataacctg
     6901 gcatgctcat tagataaagg atttcatcgt aatggatata gtgatcgcta ttaccataac
     6961 ggacagttgt ccaccttttt ttcgtagcat gttgaacagg atagttttct ttttctttca
     7021 ataagtaact ttggatgctt tttttatttt tttcataagt gaattctttt atcaaataac
     7081 cactaggcgt aatctttcga ttaataacag ttattgcttt attcggatga gacgttagat
     7141 aaacaatttt acacttttca ttcttttctc taagcatttc gcctagttcg ataccgctat
     7201 aataggcgtt caaatcgatg tctaggaaaa aaatatctga ggaatagatt tcattatttt
     7261 taatgaattc aacaaattgt ataacgttca atccctgaac gatctcaaat ttccaaggac
     7321 ttaataaaat tctggattct tccaaaattt ctactagcat tttttcaaaa accaaactat
     7381 catccaaaat aaatatacgc gttaccataa aaagtacctc cactcgatat tttactatca
     7441 aaatcatttc atacaacttt tcaacataca tgttaggaat aaattgtaat tctcttcaca
     7501 atcttttcca acacctttga aaccgatatc aaatactttc aggatgctgc ttctaaaata
     7561 aatatcaaac aaatgaatac atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt
     7621 cagctgtctg agctatgttt atttattttg ttcttttaaa aagaacgata cacatactga
     7681 tttttcggag cagagacttt ctcagcgttt gtaacaacta cagatgggat ttcacgcttg
     7741 aatggttgat aatagtctga ttggatggtt ccttctattt tataccattc atcattttta
     7801 accgttactc cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa
     7861 tggataatgc cgaatcggaa caagaagata tctgtattgc tatcgttcgg tgtttggtaa
     7921 acaaatcctt catagctgat ttttttgcca atgaattcgc tagggtaatt ataaattagc
     7981 tccataactt ctaaatagtt ttcatctgtg atcttgatag tttgcttatc cccatacttt
     8041 tctttcaact cattcatttg gtctaagtag tctgatttgt tgaaataaat actggtatcc
     8101 ggctttaagt attgtgtctg catctccgga tcccctactg attctttact tactgggaaa
     8161 ttaaatcctt tggcttctac gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc
     8221 acgattactg gcaaagctag cagtacataa gcgattccac gttgataagg ttttgacaac
     8281 ccatggtcat gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt gacccacaaa
     8341 tacagctgaa ctaaagccaa aagagcagat aaaatcattg aaagaatagc taagtagcga
     8401 taatggacat tgatatattg atcgagtttg ccagaaactt gcaagtacat catcaattcg
     8461 aaatacccag ataaaatcaa aaaccgcagc atattttccc tcctaaataa ataatgtgaa
     8521 tccaataacg ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata
     8581 tcgtttcatc atcagtagat ttttgatatc gaccattggg ccaaagacca aaaaggcagc
     8641 tacaggtgct gtgccgaaca gacttaacaa agaagatcct ataaacgcat ctgcttctga
     8701 gcaaagagac atcgtaaagg ccaataacag catgatgata atggctaaaa tctttgttga
     8761 gccgagcgtt agcataattc tagttggtaa ataagtctgc atcgcagctg cgattaatcc
     8821 gccaatgatc aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg
     8881 cccagtttga tggatcagct gattttttgg ttcattcttc tcactttgtt cttcttcatg
     8941 gacatgatga tggtgatttt ctgctacttt agacgtgatt ttttctttca atacagcgtc
     9001 tttttgaaaa taggcaagcc agacaccaat cacaagggca acaaaaaagc tgcctatcac
     9061 ccgccagctt gccatctgcc aagaattgcc aaatgcgata aaagtggaaa aaatcacgat
     9121 tggattgatg atcggagcag tcagcataaa agcaaacgct gtgtgaactg gtacgccttt
     9181 tttaacaaat tgatgaacga tcggtacgat gccgcattca catgaaggaa agaaaaatcc
     9241 taagatcgaa ccagtaaaaa tagagaagat acgattttca ggaagccatc tcttcacacg
     9301 ttctggggtc aaaaatactt gtaaggcgcc tgagataaga cagcctaata atacgaaagg
     9361 taatgcttca ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg aatttggaat
     9421 aaaagaaaac atgagtttcc tcctttttat ttctagttca ttcgctttgc aaatatacgc
     9481 ttttattcaa aaaaaacaag ttgctctgta aatttttaca caactcttat tttttaccaa
     9541 agcacttcaa ttgacttttt ctgttattcc cattaaatta aggtctcata catatcactt
     9601 ctatcataaa agggagaata aaaatgaaac aaaccactaa cttgactgaa gttcaagaca
     9661 tactgcaaca gtcatccgtt gccgtcattt atctttcgat gcctaattgc agtgtttgtc
     9721 acgctgttcg tccacgacta gaggaacttc tcacacatta tgatattccg gcacttcatt
     9781 tggatgcatt tgaaacacca gaagtagcta gtaaatttga agtgctgact gcgccagttg
     9841 tcctgatttt tcatcaagga aaagaagtct ttcggcaagc acggttcatc gactttaaaa
     9901 aaatccgtta tctactagat gaactgacag cagaagatga ttccctttct tatgaagaaa
     9961 tcttccgaac aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt
    10021 tgccttcatt tgttcaatcg ctttttattt ttattctgct gtattttcgg aatgcgcatt
    10081 tgcgtcagaa tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat caacattttg
    10141 gatcaataca tctgacggag cattcaatcg agcagcggta aagttcaaga ttcctttgat
    10201 tcctgcttcc gttaactgat tgactacttc ttgtgctctt tcagcgggta tcgttaaaat
    10261 tgctacttcg atttgttgga tcttgatttg ttctgacata tcttccattg gataaacggg
    10321 gataccgtct acgatgcgcc caacgatttc ctcattcaca tcaaaagcac aactgatacg
    10381 tatgctatta ctttgatgga atttgtattt tagtaaagca cttcccaagt tccctacacc
    10441 gatcagcgca acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag
    10501 atcttctaca tcataaccat acccacgttt tcctaattcg ccaaaataag aaaaatctcg
    10561 acggatcgtt gcactatcta cttgaacggc ttcacttaat tcagttgaag acactttttt
    10621 ctttccagca ttatttaaaa tccttaaata gcgataatac aaaggcagtc ttttagctgt
    10681 cgcctttgga ataatatgat ctttcacgaa atgacctcca cgtattgtct aattacatct
    10741 ttgatttctc catcatagca gtatttatag gaaaaaatca attccattgc tcagatattg
    10801 tgaaatttta ttctttattc atttatttat catttctgca aatctcgcag tataatggaa
    10861 tttatggtag actactaatt gaaaaatagt tttttatttg aggagaattc acaaagtaga
    10921 aaagaggatt ccatgatatt attacaagcc aatcagattg ctcgccattt tggtgcagaa
    10981 gtcttatttg aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc
    11041 agaaatggcg ctggaaaatc gactttatta aagatcattg ctggaattga agcacctgat
    11101 gaaggaaaga tcgctaaaaa taaaacagcg actctcggat acctcgctca ggacacagga
    11161 cttgattcca atgaaacagt ttggaacgag atgctgaaag cttttgaaca agttagacaa
    11221 atggaagagc ggatgcgcga gctagagatt ttcattagcg aaggtacgcc agacacacct
    11281 gcttatagtt cttatttaaa agaatacgac cggctgcaac acgatttttc agatcgaaac
    11341 ggctatggtt atgaaaatga aattcgttcc gtattgcatg ggtttaaatt cgattcttca
    11401 ttttatgatc agaaaatcga tactttatca ggcggacagc gcacacgttt agctttagct
    11461 cgcatgcttt tgcaacaccc tgacattttg attctagacg aaccaacgaa ccatttagat
    11521 atcgagacac tttcctggct tgagaattat ctgcaaagct atactggtgc attattgatt
    11581 gtttcccatg accgttattt tttagataaa gtagtcaatg aagtctatga attaagcaga
    11641 agaaaaatgc gccattacaa aggtaactat tcgaaatatc tagatttgaa agcagaacag
    11701 ttagcaagtg aatggaaggc ttatgaaaag cagcaaacag aaatccataa attagaagac
    11761 ttcgttgctc ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag
    11821 acattagaaa aaatggaccg gctggatcgt cctcaagggg atgaaaaaca agcaaacttt
    11881 ctattcagta tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc
    11941 ggatatgata atgtcacctt atctgaacca atcaatctag atatccgtcg tcaagaagcg
    12001 attgctctag taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc aatcattcac
    12061 cagatccctt ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat cggctattac
    12121 gatcaaggtc aagctgatct gcatacaaac aagacgatcc tagcagaatt gtgggatgag
    12181 catcctacca ctccagaaaa agagatccga agtgctttag gtgctttctt attttctggc
    12241 gaagatgtag aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg ggttgcttta
    12301 gccaagttag cgatgaacaa agaaaatttt ttgattttgg atgaaccaac aaaccattta
    12361 gatattgaca acaaagaagt attagaaaat gcattgatcg attatgacgg cacactgctt
    12421 tttgtttccc atgaccgata tttcatcaat cggatcgcga caaaagtcgt cgaactctct
    12481 gaaaatggca gcaaagtcta tctaggagac tatgattatt atttagagaa aaagcaggaa
    12541 gaagaagaaa tcgccgaatt gttggcacaa gatgagccag aatctgctcc tgctcccacg
    12601 aacaaatctg attattacca aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga
    12661 aaaattactc aggttgaaga agaaatggcg cggatcgatg aatggatcga tcagttggaa
    12721 caagaaatgg tcgcgccaga aaacctggaa gatcacgtaa aactcaatga gctgaaccaa
    12781 gatttagaag ctgctagaca agatcaagaa acaaaattga cggaatggga agaactaagc
    12841 ttggaattag aagaatatga aaattaacga aaaaaccaaa acagaaacca ttcatgctgt
    12901 tttggttttt ctttttttat tttatcaaac aaaaattcca tatttacctt tttcaccaat
    12961 aagaagacca attactagtc attctcacta aagcttctaa ataatagtaa tcaccccaaa
    13021 tacaaggtgt atttacccca ttatttttgg gcttattata aacagactgt gttaaaaagc
    13081 catcttcttc ttccgttgta gcatatcttt ctttcaattg tcgcataatt ttccaaacag
    13141 cagactggta taagttcttt tctgttttgt ctaatagacc tgattctatt aattctaata
    13201 atccacaaac catgatcacc atactagatg tatctttttc ttctctgcta gaatcatcaa
    13261 agatcaaatc ccaataacaa atggtatctt ctggcaaccg attcataaaa taattggcta
    13321 atattttgct taaatcaaaa tatttttgtt ctttcaaata acgataactt aatgcaaaac
    13381 cgtaaatccc ccatgcttgg cctctagccc aacaggaatt atcactataa ccttgatgcg
    13441 tacttccata tcttggtttt ccatctttga catccatata aaatgtatga taagttgaac
    13501 catcttctct tactaaataa tctgctgcat tttgaatatg agcctgagca gcttttttat
    13561 atttttgatt tccagtttct tcactcgccc aatacaataa tggaagattc atgcaacaat
    13621 ccatgatcat tctaccttgt tgtgaaggat cactaggatc tccccaagct tgtataactc
    13681 ctgccttctc ccaaaaacgc tgaagtaatg tttctgcagc atcaattgaa gcttccttca
    13741 tttttcgatt tcccgttatt ttataatctg ctacggttga taaagaatac aaaaaaccaa
    13801 gatcgtgatg attagtgata atttgacagt caatcctgtg gataaaatct atatctgctt
    13861 tttcagctgt ttgttgaaat aaaggatcac ccgtcttctc aaataacaac caattaattc
    13921 ctgtccaaaa tccttctgtc caaccaatat tgtctcttaa tggataacgt ccttttatag
    13981 gacactcgct tgggaattga tactgactgt tactgatacg tttgattaac ctctccgtgt
    14041 gtctgcgaca acgctcgatc tcattattcc attctttttc cattaattct ttataacatt
    14101 catttatcca tttttctgga tgtattatct cttcaaacat cactttatca agcatttccc
    14161 caccagcctt tagcataatt ttagtctttt tttattgttt ttattttgga tagttagccc
    14221 ggttgtccca tcaaaatcaa ttttgggacg attgttccat acttctaaac tataatcccg
    14281 atcttgatga gcaaccactc ctgttccgag ccaatgggtt ccttttccaa atgtaccctc
    14341 taatccagga ataaaagtgt tttcccatgt catcaagttt gtattgggca aacccataat
    14401 agctgtggat tttcgtttaa tagtatcgtc acttaaacag ataatacctg aaaagccagc
    14461 ttcactcacc ctatagtttt ccttgtcttc ttctatgatt attccttctt tctcaggaga
    14521 acattcaata gcaaaccctg tctcatatag aacatattct gtatctaatt ctactttgtg
    14581 tacgcgaatc tgccagcctt caaaaggaat aagccaagtt ctgatttttg cctgtaaagt
    14641 aggttcccat gtagaggcaa gtacctcttt cttacataca atgtctcgta cattatttcg
    14701 aacgatgaac tgtccatagg ttttgatgct ctcattctta aaactggtcg tgatatatgt
    14761 gtccgctcgc tgaatcccta gcattgaatc cattgccatt tctgctttca attgattcgc
    14821 tcgcggtatc gaaaaaccaa attgactaga ataagcaaat tttaaatatt tttcattggt
    14881 caatgtatgg aaatttggcc ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc
    14941 atgttgtatg agcaaattgg cttctgaaag aagtttagtt ccttcacgaa taggcatagg
    15001 catttcttta gccgtccaat actcatggtc ttcaggtagc gccaacaata aaaaaatctt
    15061 attcagccat aatggagata acgtactatt atagggttca gtcaatgtca attgagggta
    15121 ggtataacca atattcagga tacctttttc atcaaatatt tctttttgca tccaccatct
    15181 tatattccga ttgactaacc ccttgatttc acctaattta aaaggcaaaa gatcggccca
    15241 caacatagct gaccaaaagc ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg
    15301 cacatttgca ccatcttccg caaaaaaatg aatatagtct ctagaaaaat caatagctcg
    15361 gttcttatat atctggctaa tgggcttttc tggttctagt acgctataaa tcaaaccata
    15421 ataatgaaat gcaaaaggat tataataatc ttggcgacct cttgaactgt ctcgatacca
    15481 gccattttga agataacaat tttctatatt atcaaatgct tctttcagtg ctgattcttt
    15541 aactggataa ccaaattttt ggataattgt tccgacaatt atcttaaaaa attgccaatt
    15601 accatctgca cattcatatt caaaaatttg cgagaaccaa tgaataatct ggcttttttc
    15661 tcctgtatct aattttttcc ataataattg ttcatgatga tataacgcta ataacattgc
    15721 gggcatttct accatacgct ggtctctttc atgtatttct ccccaataat cgggatggga
    15781 aggatctgtt ccagctttta ttcctgatag aattaatttc aaccatcgtt catctaattg
    15841 gtcaattgcg ggccccgctc cccataaaac ccgagagaat ccttccattc ctgcaatttt
    15901 atttccatat cctacccctt gatgtatata atcaatttgt gcgccacttg ctcgataaaa
    15961 cggctggagt ggttgattta tctttttaaa taagtcaaag taatcctgtc tggtccttaa
    16021 tagtggtact ggatgtttca ttgtattttc tcctcttttt actaccaaat agctaattcc
    16081 gactgttcta atcgaagtaa gccttcaata aaaaaataat cagaatagat cagtggcaca
    16141 ttataagtca gttctctatg gtatgcttct gctccgcctc caatgatccc atcttcatcc
    16201 atattccaat ttgcatagtg cttttctgtc ttttggagta tattgatagc agcttcatga
    16261 tacatttttg atcgtacaga ggaaacatat cctgctaatt ccagaagacc gcatgcagcg
    16321 catactccag cactggtatc aatattcgaa tcttttacag caaaatcaat gagaggaata
    16381 taaccagttt tttcgatttt ctgaataaaa tttactgcta tcttttctgc tatagctaga
    16441 tagtacggat tttttgtgta acgataactt aacgcgaatc catataaagc ccatgcattt
    16501 cctctgctcc aacaagaatt ctcattgtat ccttgcccgc caagtaaccg aatatatttc
    16561 cctgattctg catcaaattc tgcgatatgc ccggttgatc catctttcct cactagatac
    16621 ttagctgttg tatctgcatg ctgccgagca attttagaga acctcggatc accagtttcc
    16681 ctcgaagccc aatacagtaa tggtaaattc atcatactat caataatcat ccaaccaggt
    16741 ttatggtcat tccatgctac taaatagttc cctgaaatat tatagcgtcc agcaagtacc
    16801 gtagctgcat gtaaccctag ttgtcgacta tgtttgtttt gagactgttg ataatgtgca
    16861 atagccgatg gtaagaataa aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg
    16921 gcagattcta ataattgttc ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc
    16981 ttgtgaaaga gctgccataa aataccactc caaaaaccat tggtccacca gtcgattcct
    17041 ctgtccttta ttacgtctgt atattttccg ttgtaagaaa tatatggaat tttattcccc
    17101 attctgtcta attccgcatc tatcttttca gaagctacat tcaaaatttt tcttgaccat
    17161 tccttcgtct gttcattcat ggatatcccg ccattctttt aatttttatg aatgtggagt
    17221 tgctcctaga acgaactaga tggttaacat tcgttatttt ttttacttat ttgcagctcc
    17281 aatgtcattc tccttaaatt tagactgcgc ccacacataa atcaagacca ttgggatcac
    17341 tgaagttgct aaaattagca taattgcgcc ataattaatt ggctcacctg tactagaaac
    17401 cgtcatattt ttgataatta agggaaccgt atatttatct ttatctgaaa gcatgactaa
    17461 tggaaataga tattgattcc aacttcccat aaagataagt aaaccactag taataatcgt
    17521 aggtgataaa atcggtacag cgattttcgt aaaaatcacc cactcattgg caccatcgat
    17581 tctggcagcc tccatcagtg ctgtaggaaa ttgactgcta tattgtcgca tcataaacac
    17641 tgaagttgta ctggcaatcg aagggatgat cacacctgca taagtattcg ataatcctaa
    17701 acgactcata atgtaaaata aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag
    17761 cataactgta ttgaacaaca gtttattccc cataaaacga tactttgcta atgcataacc
    17821 cgccattgta gaactggctg tagctaaaac tgtactgatt acagcaataa caatcgaatt
    17881 ccaaataatc aagggataat tatatctcgc catgatggct tgccagtttt ttaatagcat
    17941 atccccaaac ttgaaattga ttccgccact taaaatttct tgattacttt gcgttgaagc
    18001 tacaatcata tataaatatg gcatcaaaaa tattaaggca ccaaacacaa taatgacgta
    18061 cgtaaaaatt ctgctttgta tcttttgttt agtcattttt atcacctccg aatttaaatt
    18121 gaatagccga taatataaca gatacaaaca ccatcgtcca ggcaatagca gaggcttttc
    18181 caaaatcaac catttcaaaa cttgttttat ataaattgac tcctacgacc aaagctgaat
    18241 tttctggtcc attggaagtc ccaaagaata tatttgggat attgaaatta cccaagccat
    18301 tgatagtggc taaaactgtg gccattaaaa tgattggctt tagcatgggg atcgttaata
    18361 aaaagaattg ccgaaatttc gatgctccat ctatttcagc actttcgtat atctctttag
    18421 aaatcccttg aagaccagct aaaaataaaa tagtgttata tcctacacta agccaaatca
    18481 tcgtgatgat cattgatatt cgtatccaaa aaggatttgt taaccactgt acaccgttaa
    18541 ttcctagcgc attcaaaaat ccattgaata acccagtcgg attgaaaaaa gtcatgaata
    18601 cggaagctac agccaccgtt gatgtaatgg ttggaatata atagacagtt ctaaaaaatc
    18661 cttttccttt taactgtgct gaattcaata aaacagcaaa gtaaatagct aatagcatcg
    18721 ttattggaat tgatcctgcc atgataatca aaacattcgt gtaagattta ataaacaatg
    18781 gatctcggaa gatagctaga taattgtcaa atccaacaaa ttcaaatgag tagccaattt
    18841 tcataaaact aaaagcaaac gagataatga tgggaatcaa aatcgtcaca ataaagaaaa
    18901 taacaaaagg tgtgaccaat ccataagcac tttttttatg agacgtatct gttgcttttt
    18961 tcttgctgtt ctttattgtc ttgcttggta acgaagattg catcatcgtt aagtcctcct
    19021 aatatttttt aatcaccagt taattttacg ccgtacttat gagcaaaatt ttctgcttct
    19081 tttagatatg aattttcaaa atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa
    19141 taagacgtag catctgaata agtggctggt aaaacagtat ttgatgtatt ttcattgatt
    19201 ttattcaata gttcatagta tttttgtcct ccttagtagt caaacttttt ctcagctgct
    19261 tctgtttgat aggccaatga gttagatgta gccacgccca gctccagtgc gttggctaag
    19321 cattcttcat ctgtcagcat aaatgtaaga aattgttgtg ccacttgtgg atttttggca
    19381 tctttaggga cataaaaact tgatcctcct gatactgcac cttttcctgg attttctttt
    19441 gaaaaaggaa tcagtggagc catgcgccat ttgtcagatg catcaggaaa attcaaagac
    19501 atattcgtag cataccagcc gccagcaaca aacattgcag attcttgaaa tgctttctca
    19561 gcatcttgtg caccgtagaa atgtacaata tctgagtcga tcatttcttt gattaacttt
    19621 gcagcatgtt tcgcttcttt agtattcaga cggatatttc cttcttcatc aagcaaagat
    19681 tgattctgct gggctagcat gttgataaac aattcttggt ccccagattc tggcaaggca
    19741 attccaaaac taccagtttt ttcttttaat gcttttgcca cctggatata ttcttcccaa
    19801 gaactgattt cttcaaaatc tacacctatt tcagcaaatt tttttggttg ataataacaa
    19861 agtgctgggg taaaatcact agcccaagga ataatcgttt cattactcgt ttgttgacgc
    19921 aaaatattca atttttgatc ataaaactga tcgccatatt tgtcataaaa tccatattca
    19981 tcagcactat aaaaagcatc ttgaaatttt tccagcacac ctgctgctga agtgtctgcc
    20041 atggagataa tatccggcat atcttcgtgt gttaccaatt ttggggtgat catttgtact
    20101 gttgctgttc ccgaagctac attcacaatt tttaagtcaa tatttgctcc agttttgcgg
    20161 ttatagattt ttgcagcatt ttcaacatag tttgtcagtt tgccgtttgc gacatatact
    20221 tctaatgtag tggctttttt tatcattgga tctgcgatgt ttgctgttac ttcttcgtta
    20281 gtttttccac atgcacttaa gctgaataat agggtaatgt ttagcaaagc tatcaccaat
    20341 ttgtatcttt ttttcatgtc tatcccttct ttcttttcaa gttcttatat aaaacgcttt
    20401 cacttatgta tattaacgga attagtaaca caaatattta tataaaaaaa tattttactt
    20461 gtcgttttgg cttttcattt ttgttataat agtcattaat tataaataga aagcttttta
    20521 tataaaaaga gaaaggaaag tggttctttt gttaacaaac aaccaatcct ccaatcttgt
    20581 caaaaaaaca ctaacctatt ccatagatac ccatcttcat ttatctgctg ctccctattt
    20641 tcgactacta ataaatgaaa gcgctaattt atatgctgaa tttgcacata aatctataga
    20701 actagataat ccttcccttt tattcattga taaacagcaa gaagtccaaa tcttttctac
    20761 tcaaaaggaa acacaaactt ttatcattat ccagttttat tttgatgagc aattattagc
    20821 acatattacg ggacaatctt tttctataga tcaagaaata acatctatca ttacttttct
    20881 gaaaaatgaa cagttcaaaa ttgattatct aaaacatagt aagccagcta tttcctatga
    20941 tcaggaaatc aattcttttt tcatactatg ccaacagatg agcattcatt tgctttcctt
    21001 attagttact tattgtctgc aaaaaattca attgtcttcc ttaccaaggc attatcaaca
    21061 ggaacttgct ttattgacaa taccttcaaa tgagaaagtc attcctaaaa aaaagcatat
    21121 tctttattca gatgaattag ttgatccaat catacgatat ttagaagaac atctttcaga
    21181 aaattttttg cttgaacaat tagcccaaat tttttttatc agtccagcca gcttaaaaaa
    21241 acattttaga aaagtgaatg gaaaaagtgt tattagctat tttaaagaac taaaaatgga
    21301 aaaagcaaaa cgtctcgtgc tagaaaatga attaacctat acagaaattg cagatatatt
    21361 aggatttcat tcgatccatc atttttcttc agcttttaaa aaatatacag ggttatcccc
    21421 ttcaaagtat tatctatcct taaaatagaa aaaaattttt aggttttatc cacaaattat
    21481 ttctctatat tttctgtcta ttcaaaataa acaataattt tccggcacaa ttatttgctg
    21541 ttttttacca taacatagta cgatcaacgt agaaaaggag atgtttttat gaaagttgtt
    21601 gtcattggcg gtgtagctgg cggtccttca tttgccactc gtttccgtcg attgaatgaa
    21661 gcgcacgaaa tcattatcta tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg
    21721 ccttattatt taggaggtgt gatcactgac cgtgactcgc tgatcgaacg tacaccagaa
    21781 atattgaaaa caaaaaacaa catcgatgtc tttactaaac acgaagtaac agcaatcgat
    21841 ccttctacta agcgattaac agttaaagac ctatccacaa atgaagaaat aaaaacagat
    21901 tacgataagt tgatcatctc ttctggtgct agaccagatt atccggatat tcccggagtt
    21961 tttgaagcag aaaacggctt tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg
    22021 ttccttgaag aaaaaaatcc acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg
    22081 gaattagctg agaatttcaa gcatcgcggc ttagacgtga ctttaatcga tcaattgcca
    22141 caagtcgctt tcccttatga tccagaaatt gctaatttag tttatgacaa attgctgaaa
    22201 gaaggattag ccgttcattt agaaacaaga gttactgaga tccgtgataa aggtcgagaa
    22261 atagtattat cagatggctc cgtcctttct gctgatatgc taatttttgc tgttggtgtt
    22321 tctccgaaga atgaagtggt gaaagcaacc ggcatacaat tatcagatac aggtcagatc
    22381 attgttgatg accagttaca aaccaatctg ccggacatct atgcgattgg cgatattatc
    22441 gaaacgacta gtgtagtgac tggtcagccg atccaaagta tgctttccag tgcggccaat
    22501 cgtcaaggac atatgctggc agatatttta aatggtacgc ctatgcgcta tcgcggatat
    22561 attggtgcag gtgtcgcaaa aatctttgat catacagcaa gttatgcggg aatgacagaa
    22621 catgaattaa aagcatcagg cataacaaat tataaaactg tttttatcac tccctttgac
    22681 catgcctatt tctatccaga agctacaaga ttaaatctaa agctgatttt tgatgcagat
    22741 agcggtcgta ttttaggtgg acaagcattt ggagaaaaag gtgtcgataa acggatggga
    22801 gaactttctg tagcaatcac cggaaacttg acagtctttg atttgcctga tttggagttg
    22861 ccttactctc caccgtattc tactacccgt gacccgttga atatagctgg ttatgtcgca
    22921 atcaatcaaa tgacgaatat cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat
    22981 ttgaaagaag cgttcttttt agatatacgt gaacctaata aagcaccttc tggcagtatc
    23041 tcggcaacaa aaaatattcc aatgaatgag ttacgagatc gaatcaatga aatcccaaaa
    23101 gataaaaaag tttacattac tttcagaaga ggattgaata cttatacttc tgcccgaatc
    23161 ttggcaggtt tgggtatcaa agcgattttg attgaagaat aaaaaaacga ccgcggtggg
    23221 tagccgcggt ctaggagtta tttttacttg aggagtaaaa atgaaaaagt atatagggtt
    23281 gttggttggt atgtatatat aatagaagat aaatgtgaag aaattgtgca ttatttcttc
    23341 acatttatta ggttatagtt taagaaagtt taaacattgc gcaatttaat tattttagct
    23401 gtttcttttc ataaaaaaac atgttgtgac tctgagacaa atcatagtcc atagtgatgt
    23461 ctctagagta actacgccta atattacgag gcatcatatt aatctatcag cactagtacc
    23521 taaactagca tccctttcga atattttttc ttatataata aagtttgctt tcactcctct
    23581 aatctcacca aatcgactgg cttgcgttgt gtgatactag gaactgattg gtttagaacg
    23641 acttttctat tgtaatcata tttttcgcca tcctcgttgt cttctaatcc ttcaatgaat
    23701 gatccctaat gtgtgtatta aataatttaa tttttttgtt cctatttatt taa
//
LOCUS       DAPBBG010000043        23573 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPBBG010000043 DAPBBG010000000
VERSION     DAPBBG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.043"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(380..2566)
                     /locus_tag="RD477_001959"
     CDS             complement(380..2566)
                     /locus_tag="RD477_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDT7634315.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2784..3467
                     /locus_tag="RD477_001960"
     CDS             2784..3467
                     /locus_tag="RD477_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDT7634316.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3548..4267)
                     /locus_tag="RD477_001961"
     CDS             complement(3548..4267)
                     /locus_tag="RD477_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7634317.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4579..4785)
                     /locus_tag="RD477_001962"
     CDS             complement(4579..4785)
                     /locus_tag="RD477_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634318.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            5029..5631
                     /locus_tag="RD477_001963"
     CDS             5029..5631
                     /locus_tag="RD477_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDT7634319.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5772..7052
                     /locus_tag="RD477_001964"
     CDS             5772..7052
                     /locus_tag="RD477_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7634320.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            7065..7793
                     /locus_tag="RD477_001965"
     CDS             7065..7793
                     /locus_tag="RD477_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634321.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7862..9385)
                     /locus_tag="RD477_001966"
     CDS             complement(7862..9385)
                     /locus_tag="RD477_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDT7634322.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9637..10818
                     /locus_tag="RD477_001967"
     CDS             9637..10818
                     /locus_tag="RD477_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7634323.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10878..12506)
                     /gene="treC"
                     /locus_tag="RD477_001968"
     CDS             complement(10878..12506)
                     /gene="treC"
                     /locus_tag="RD477_001968"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDT7634324.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12903..15614)
                     /locus_tag="RD477_001969"
     CDS             complement(12903..15614)
                     /locus_tag="RD477_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634325.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15624..16325)
                     /locus_tag="RD477_001970"
     CDS             complement(15624..16325)
                     /locus_tag="RD477_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634326.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16511..17113
                     /locus_tag="RD477_001971"
     CDS             16511..17113
                     /locus_tag="RD477_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7634327.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17194..18207
                     /locus_tag="RD477_001972"
     CDS             17194..18207
                     /locus_tag="RD477_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7634328.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18316..19233
                     /gene="rbsK"
                     /locus_tag="RD477_001973"
     CDS             18316..19233
                     /gene="rbsK"
                     /locus_tag="RD477_001973"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDT7634329.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19261..19656
                     /gene="rbsD"
                     /locus_tag="RD477_001974"
     CDS             19261..19656
                     /gene="rbsD"
                     /locus_tag="RD477_001974"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDT7634330.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19656..21029
                     /locus_tag="RD477_001975"
     CDS             19656..21029
                     /locus_tag="RD477_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7634331.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21386..23530
                     /locus_tag="RD477_001976"
     CDS             21386..23530
                     /locus_tag="RD477_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7634332.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         6872 a         4611 c         4230 g         7860 t
ORIGIN      
        1 gtataggtca tttgtactca ctccttataa ttctttggtc ggaactattt tgagtgtagc
       61 acaaatgact tttttagttg tctagcttaa ttttacaatc ggcgaaatat aaaaaaaaat
      121 caaaataact gattatttac gtttgaataa tgagaatttt tctatatatt ctctattttg
      181 ataatagtat tctcattttc aaaataaata agggaggtgt gacaaaattc ttgtcacacc
      241 tcccttttcc gaataaactg tgttccagaa atagcttctt cgaaaataag ctgaaatttc
      301 acaaaaattt gagaagcaat tttcgtacat tccttcttct ttcttgaaac tgaccacttc
      361 tgtcacaccc tctcttctat cactttcctt ttttcacacg actcccggtt tctttgataa
      421 atctcgatac tttcaatttt cttgaatttt tcatttgagg tacacataga tacagacgat
      481 ttcttgctct agtcatcgca acataaaata gtcttcgttc ttcttctatc gtcgtcactg
      541 gttctgcttt tatttcatta aagtcgatta ctttcttttc tgtcgctccg ttataaggca
      601 aacttccttc gttacaacca atcaaaaaga catttcggta actaagacct ttacttttgt
      661 gaatgctctg gatacatagt gcatcaagtt ttccttgtct gtaagcaatc aagcgggttt
      721 ttaattcttt agctgtcgct tgggtttctt gcgataaggc ttggagtctt ctgaaagtag
      781 catgctgctt tacttcttct cgtatccatt cttgatcact ctggtcgatt tccttagcta
      841 tacgtggcag atgaagatag ctttgaacaa gcgaatagat ttcttgatgc tggtctgttt
      901 tacgcaacgc ttcctcaaac tctcggattt ctgcagattt cgattttaaa ttggggctta
      961 tcgacaaaat ataatctgca acggctatat tttgatattt aggagaaaag tattgatcgc
     1021 attcactttt tttcaggtag cagtctgcca tcagctgccg gagcaattgc tggtaggcct
     1081 gatttttctt ttgcagattc agtgctgttt gccaatgaac aattttttga tagaaatgtt
     1141 ggaaagactt gataccaaag ataccttcat atggactatc tgcttccagt acaaaaggaa
     1201 tattcgtttt cagcagagat tggacaagct gctgcgtttg tgaagctgat cggtataata
     1261 tgattgattc cttatatttc ccattttttt gaataaatgt ctgaatttgt ttgattagcc
     1321 actgactttc tgctttagta tcagaaaaca aataggcttc cataacacct gcttcttttt
     1381 gtgctggaat catcgccttt ggtatccgat catttttatt atagctgatc aatctatttg
     1441 ctccttggac aatagctgcc cgagaacgat aattagttaa taagaaaaga gttttggtag
     1501 gatattcttt ttcaaagttc ttgatgtatg ctggctctga tcctctgaat ccatagatac
     1561 tctgatcatc atctccaaca acaatcaact tgatcctgtt ttcctttgtc atccgtttca
     1621 cgatttccca ttgaagtgga ttagtatctt gaaattcatc aatgaataga taatcatatt
     1681 tttggccgat ataatgctgg atctcctcat tctccaaagc gcttttcaga ttttggagta
     1741 catcattgaa ctctagcaat tgctggcttc ttttttgttc ttggagttcc gtgagcaatc
     1801 catacagtcg ttctgatatc ggctgtcgtg cattcttatc aatggaatca tatttttctt
     1861 taactaactg aaccatttct tctactgaaa ggttcatatt ctgccaatag tctaaccgag
     1921 aagcaatcgt tttttctcca tattgatcaa atatataaag attatattta cgtatcagct
     1981 gttgcaaaac agcattcact ttttgtgcag tatcctttga aaagtctcct tgtattcctt
     2041 gcattgctac ttcaggaaat tttcgacaaa tttcattgta tatcctaaaa aagatactat
     2101 ggaatgttcc gatatggaca gcggtttttc tctggctgaa attatctact cgttttttta
     2161 gttcatttgc agcgttcctt gtaaaagtga tcattagtat gcgttcttcg tctacttgtt
     2221 tttgcgtcat cagataaaca gcttttgcac agattgtttg cgttttgccg ctcccagctc
     2281 ctgctatgat tcgaagatgt ttcttcatat catatgtgat agctgctttt tgttcttgat
     2341 tgaagcgatt ataggaacgt ttctccatca ttttaaagaa agagctgttt ccttcacttt
     2401 cttttacttg tacttcttcc tcaataagtc cgggtgcttc gccataagcc gcttcatagg
     2461 tagcaatgat ttcttcttgt agccgaaaga cttgttgaag ctttttatac ttgttctccc
     2521 agcgtttcaa ttctttttgt aatcggagaa tttctttttc attcaactga ttgatttcct
     2581 tcccatcttt cttctttgaa tttgtttatt tcaagttggt tatttaaagt ttcgttgtct
     2641 attttttctt tatactaaca gacctttcta aaaatgaaaa tgggaagcga tatgatttta
     2701 gttttctgaa aatttttcct ttttttcaat cgtttatgct aagataagga agacaaaaat
     2761 attcataaaa ggtgatgaaa aaaatgcaga cttcctttca cgaggggcgt acaataggac
     2821 ttttgctgac atttatcgga ggagccatgg acagttatac ttatatccaa tatggtgctt
     2881 ttgcctctgc tcaaactggc aatatcattc tatcgatcat ccaagcattt gacggagaat
     2941 ggctaagtgt tggtaaaaaa gtattgtcta cccttttctt tttcttaggg attcttctaa
     3001 caaagtatct aattgattat tttagaaaaa aagaaaaaca tttctggcgc ttgtttgttc
     3061 tatattatga agcagtgatt tttttctttg ttagtttgac acccgtccat gagcatcccg
     3121 cattagtaac gattttgatt gcttttactg catcgatcca gtgggtagct tttgataaaa
     3181 tcaacgggcg tgcctacact aatttattta caactggaaa tttaaaaggg gtagcaacga
     3241 acttatatga ttattgtatt tctaaagaaa aagcagattt tgatcgattt ttccactatc
     3301 taagtgtcgt tcttgccttt atcgcaggag ctattgtcct tgttttctgt catcatctgt
     3361 ttggcgccca ttcgattttg atcgtttcca ttttattttt gatcatggcc ctcactcaga
     3421 catttcattt atggcaattt tatcatacga atcggttcac tatttgatct caatgaatca
     3481 aaaaaactaa gcaagatgct gctcacagag aaatatctgc gagtgagcaa tttgcttagt
     3541 ttttatatta tttagttgta aagattccct ttgctttcgg gttatttgta gtgtagcgga
     3601 tcgactgggc atctgcctct tctgtcattt tctttgcccg catcttgatt tcttgtacga
     3661 tttgttctcc acatgccgga tcggctacga aatcatattt gtccccatcg atcactattt
     3721 tgggacatat atcaaactgt tcataccact cttcgtatcg tcgattgagt tctttatagt
     3781 aggaatagag ttcaggatct tgttctagct gttcatattc ccggcctctt tttttgattc
     3841 tcgctaacat agtttcaaag gatacacgaa tataaaccag tagatctggt ttcttttgtg
     3901 gcgaaatttc ttctaactcc ttcagcatcg tttctaaaag accttcatac tgctttactt
     3961 ctacttcaga aacacgccct aaatctgcat tcaaatgaaa cagcatacta tcttcgtaga
     4021 tggagcggtc tagtacgttg tcatcatggc tgaaagcatc tttgattgcc aaaaagcgtt
     4081 tgttcaaaaa gaaaatctgt aataaaaaag tatacttttc tggattagaa taaaatagcg
     4141 gcaatacttc attatcttcc acgttctcat aaaaaggttt tgtttctaaa tcttgggcaa
     4201 gcatttcagt tagtgtgctt ttccctgcac cgatcgttcc tgcaacaatg atcacaatta
     4261 tcggcatcct tcctgtaata aaatctgttc ttcattttac tacttcattt atagaaataa
     4321 aagccttgac tttatctgct ccttaaaagc tacttctcta aaagtagcca aaagtaactc
     4381 ctattcatga aaataaaggc tgtgacaaaa gtcttgtcgc agcccttttt tcaaataaac
     4441 agtattctcc tgccaacacc tcggtcacaa ggcaatattc ataaaacatg aaaagctgtt
     4501 ttttaacatt attccttgta actcagagct aaacgggaga ctcacaacct ctttatgctc
     4561 tcaacatttt atctgttttt attcttctat tgtacttgat ttgcgtacaa ctaactgcca
     4621 gtgcagatta tgagcggtat cattgtcttc tactgcgcat ttcatgacac gtatcatcac
     4681 actattttca tagatttttt ctatttttcc ttttattggt gattcaaata aatcagattt
     4741 aaatgcatat gtttcgccga cttcaaacaa ttgttcattt tccataagaa tccctccatc
     4801 accttttata aggtcagtaa ttttttaaca taaatcactc gaaaaagcac aaacttgtca
     4861 taaaaagtac ttttcgtatc aaaactgtca ctttttttgg taaggttctt ggaaaacgaa
     4921 gattttttta ggagatcatg acggttttga tgtcaaaacc actttttatg aaacaaaaga
     4981 cgttttttat ttttttagca taaaatttgt ggtggaggta cgtatgagat gaaagataaa
     5041 gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga agatatggtg
     5101 tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa actagcggtc
     5161 gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac ctttctttct
     5221 tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt cgtttgtctg
     5281 ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct gcaattgact
     5341 atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact gcttcgctat
     5401 ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc attattaaag
     5461 aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc agcttcttac
     5521 ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct tcctattaca
     5581 aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta aacaattaac
     5641 taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg aattcaaaat
     5701 ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa aatcaatggg
     5761 agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac acaatttgta
     5821 tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc attctgattt
     5881 cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct ctaattgcta
     5941 taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa ttggtttacg
     6001 cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc atcggcgatt
     6061 tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat tttcacattg
     6121 tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg ttagagactt
     6181 ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata ttatttacat
     6241 actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc ttagctttga
     6301 atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt tattattgga
     6361 aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg atgcaacagg
     6421 attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa catgattacc
     6481 aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa ggattacggt
     6541 cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt ccggccaatc
     6601 taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca ttaaaactca
     6661 tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca atcgaactgg
     6721 atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac aatgcgattg
     6781 aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag aagaatcatt
     6841 ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac cagttgaaac
     6901 aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg tttatcttgg
     6961 atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa aaccagcgtt
     7021 tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt cccatttata
     7081 tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaaa aatagaatca
     7141 tgattgaaaa cttagacagt tatgtccata tttctacctc tgatccggaa gaacttctca
     7201 atgcagccaa gatcagaact tcttcttttg gtatttactt tttagatatc gaactagaag
     7261 acagtgaaat ggatggtgta gcgatcggta aattgatccg tgaattagat cctttaggaa
     7321 aaataatcta tgtgacttcc catacggaaa tgtcgatgaa aatcttaaaa agcaacatcg
     7381 aaccgactga ttatatcgtc aaagaagatc tttatgattt aaaagaaaat gtcgagcata
     7441 tcctgagcaa aattttcgaa gattttcaaa tgtcccaaat ccaaaaagat atcttcaaaa
     7501 tagagtttaa tgatgagatc aaatttttac cgatcaaaga catccaatat ttttctactt
     7561 cgctaggagc gccacataaa ctggaagtcc atttgactca tgcccaatta caattctatg
     7621 agaagatcaa agaaatcgag aaaatgcatc ggaattttat ccgttgtcat aaatcctatg
     7681 ttatcaatac acaaaatgtg cggtcgatta ataaaaaaac cagagaagtc acgttggcaa
     7741 acggcgagac catcccagcc tctatccgtg gattaaagaa attgatttca taaaaagaac
     7801 cgaatagttt ggaccgacta aatgaccagc cgatccaaac ccttcggttc ttttttgcct
     7861 atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct ggataaaatg
     7921 ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct gcatataaat
     7981 gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata tatctaaaag
     8041 cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt cctgcctcgt
     8101 aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt ccatccttaa
     8161 acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt tgatagccct
     8221 tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt tctttatatt
     8281 caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc caagttcctg
     8341 cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg ttgtagacgc
     8401 tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct gtcgtttttt
     8461 tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt tctatttgtt
     8521 cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct tcgtttgcta
     8581 acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg attccatgtt
     8641 ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca ctagaagaaa
     8701 gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg aatgcagcat
     8761 gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa agctgatgga
     8821 gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc tgtttttctg
     8881 ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct aaacttggag
     8941 ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt tcatggtcat
     9001 gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct tttatcgtat
     9061 ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc atgttttcat
     9121 cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca gaaggttcat
     9181 aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca tccccgacga
     9241 ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat ttttctttac
     9301 tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa accagcatgg
     9361 ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc tttcatttga
     9421 atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag gatttaattt
     9481 atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag aaagtttata
     9541 tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat acccgctttt
     9601 gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac agatacaacg
     9661 aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg aatagttatc
     9721 ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa gcctgtcgga
     9781 atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta ttatcttttg
     9841 gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt cattatcgtt
     9901 tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc ttttcttgaa
     9961 gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt tcaagagagt
    10021 ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt tttcgcttct
    10081 attgacgacg tgacgtctga tatagctgga tttattggaa attattggga aagtggcgca
    10141 caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt atttactggt
    10201 ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc agttttagcg
    10261 attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc taatcaacaa
    10321 ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc agtctactgg
    10381 gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc tgctgggttg
    10441 ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt gatcattgca
    10501 ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc cgtacaactt
    10561 ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat ccatccgatt
    10621 acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg cgttattttt
    10681 ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg taaattcaaa
    10741 caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc attttctgaa
    10801 gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc gtaggaacga
    10861 cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg ttttagctcc
    10921 agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc ttctgtgaat
    10981 ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac aagcagctgc
    11041 tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc ataagcatga
    11101 taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt cttgtaatag
    11161 gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc tagcttgcct
    11221 ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc tgaccattgc
    11281 atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc ttctttttct
    11341 gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc aatatagtcg
    11401 gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata aatatagggc
    11461 gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt tcgatactga
    11521 tcgatagcta caaatcgatt gagcgcacgt ggctgatcat gattattaaa aaatagagca
    11581 ttccagccgt taccctcact catcttttct ccccatgtat gaaatagtcg ctttaattct
    11641 gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac tttcaaatga
    11701 tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa gatacaattt
    11761 tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt cccaaacgtt
    11821 gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata ttcaggtttg
    11881 ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt gataacatcg
    11941 aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa tagttcctct
    12001 cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa gtgcaaataa
    12061 tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga tacccaatcc
    12121 gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc tagtgctttt
    12181 ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat gatttctatc
    12241 cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc aaagatagga
    12301 tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct tggataaata
    12361 gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt ttctttgatt
    12421 ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg atagatttgg
    12481 taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga tttattacct
    12541 accacaactt atatatacaa gttatcattt attattataa ctgaaccgaa agcgtttgta
    12601 aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag agatcagaca
    12661 tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc tataaatggt
    12721 gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg aggagctaat
    12781 tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct ctgattcatg
    12841 gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca caacctctgt
    12901 ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt gaaatgggta
    12961 acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac ataagcccac
    13021 catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa gatgaagtca
    13081 gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa gatgatattt
    13141 tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt tattgtagat
    13201 aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac gataaaagcc
    13261 agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc cattgattct
    13321 tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc
    13381 agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg cagcaaaaat
    13441 gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga catataaata
    13501 aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc tttttgatct
    13561 tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc tactttttgc
    13621 gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc agttaaatgg
    13681 ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact ttgcgaagca
    13741 tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc atttactttt
    13801 tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga tgaatccaat
    13861 gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac agaacttaga
    13921 gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat tccgataatc
    13981 gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt ttgattaaag
    14041 ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt
    14101 gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa aacgatcatt
    14161 gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca
    14221 taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa aatgatcctt
    14281 ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga ggctagactt
    14341 gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc ctttaatgtc
    14401 ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg
    14461 gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg atcagacaga
    14521 tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta tgatggttta
    14581 gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga ggccagttca
    14641 cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg ttgagataat
    14701 gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc tgactgttgc
    14761 gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca
    14821 tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt cagctcttct
    14881 aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt ttgctccatt
    14941 ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa atcaagatat
    15001 aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa ataatcgacg
    15061 ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa tggagaacgg
    15121 acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat ctgataaata
    15181 tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg gtttggtttt
    15241 tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt gttataagaa
    15301 aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt tcctgtttct
    15361 acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa gccaccagta
    15421 gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat cgttttttgc
    15481 atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat gacaataagg
    15541 atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc tttaagatat
    15601 gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga aggatgatca
    15661 tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc agcgatgggc
    15721 gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct gctagcgttt
    15781 tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca
    15841 cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg
    15901 cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac ttgcctcaat
    15961 acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac attttctttt
    16021 gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc tgttcgtcgg
    16081 taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat aatgatctga
    16141 ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt tcctgctcca
    16201 cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat atcgtcattt
    16261 gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac ttcgacatac
    16321 ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat cgaatgcgtt
    16381 ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg aatcctttaa
    16441 tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt tatttgaaag
    16501 aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa aaactgcttt
    16561 tgtcgcgatc gacatgcaaa aagggatcgt aaataacggt acactctctc cacacactgc
    16621 acaatctatt ctgacaaaca atgatttgtt agcaaaaaca ttgaaacgta ccgctgcctt
    16681 gaaagtttta gtgaatgtag acatcactac tttctcttat ctaagtcatc agacagatgc
    16741 tggaggaata tctgctttgt ctgctccggc agaattcact gacttgttgt tgaaggattc
    16801 cttgaaagat acaaacaata tgttgactat cacaaaatac aatccaagtg ccttttttgg
    16861 tacttctctt gatcttcaac ttcgccggcg tggaatcgaa actatcgttt taagcggtgt
    16921 agcgacaaca aacggtgtat atgctactgc attagatgcc ttccaacatg ggtatcatgt
    16981 tgtacttgct gaagatgctt gcagtgatcg tgacaaagaa tcccatcaat tgttcataaa
    17041 aaagatattt ccgaaaactg cacgagtacg ctcaacgaaa caaataatcg aagctatcca
    17101 acaaagcaaa taagtactgg cacacgtgat tttcatttac ttctttttga tttcatggga
    17161 aaataaccac aggatttaga taggagtatg aacatggtga aaaaaaagat cacaatcaaa
    17221 gaagtcgcta aactttccgg cgtttcaatc acaactgtct ccttgatatt aaatggaaat
    17281 acggagaaat tcagtccgaa aactgttcag aaagttttag ctgccaaaga agaattaggc
    17341 tatcagcctg actattttgc tagacaaatg attaccaagg aaacgaaaac aattggtgta
    17401 ctcgttcctg atatcacgaa tccattcttc agtacactga tgcgaggaat tgaggatatc
    17461 ttgtataagc agaatttcgt cacgatatta tgtaatgctg attctgatca tcaaaaagaa
    17521 atcgaatatc tagccgagct cacacgtcga ggtgtggatg gattcattat cgcgacttct
    17581 gctgtctcta cagatgcaat caacgaaaat ttgaaaaaac aaggccggcc cttcattgtt
    17641 ttggatcaga aaaaatctga aggatttagt gatgccgtac gaacagacga ttttcgcggt
    17701 ggttacctag cgggcatgca cttgcttagt ctaggtcatc aaacgatcgc ccttgtctat
    17761 cctgaaaatc cgccggaaaa tgtccacgct cggattgagg ggtttaaatc tgctcttgat
    17821 gtttaccaaa tctctcacga ccaactgatc ctcttaccta cacagttctc aaaacaaggt
    17881 ggctaccaaa tcacagcaga acttcttgat tctgctgcaa ctggtgtgtt tgctttaaat
    17941 gatgaattag cttttggatt atatcgcgga ttagaggaat ctggaaaaag cattcctgaa
    18001 gactacagca ttattggcta tgataatata gatatgtgcg agtatatcaa gccgaaactg
    18061 acaacgatcg cacaaccgat cttcgaattg ggacaaacat ctgccaaatt attgttagat
    18121 cggatccaat tcccagaaaa agaatgggaa gaaaaaagat tacctgtacg attagaaaaa
    18181 agattttcaa ctgcaccctt gaaatagctt tcaaggtgtg ctatattttt atcgaactat
    18241 taaaacgttt tactaaaacg ttttaaaaaa taatttttcc catcaaaata aatgagatag
    18301 ttggaggttt gtaaaatgaa cacggttaca gtaattggaa gtattaatat ggacactacc
    18361 cttcgtttaa ccaacatgcc caagccaggc gaaacaatgc atgcgcatga gatttttcac
    18421 gctggcggag gtaaaggtgc caatcaagca gtggcagcca aacgttcagg cgcacggaca
    18481 tcttttattg gaggcgttgg ggctgacagc gaaggacaac agttattaga tctactgaca
    18541 aaagaaaaca tcgatacatc tgggatagct gaaatccaag gcgccacaac cggacaagca
    18601 atgattatgg tagacgcagc aggagaaaat agtatcttga tccatgcagg tgcaaataat
    18661 gctttccatg aacaagaagt cctaaaaaac aagcaactga ttacaaacag tgactttatt
    18721 attgctcagt ttgaaagtag cttagatgcg acgatcctag ctttttctat tgcaaaagac
    18781 gcaggaaaaa caacgatcct gaatcctgca ccagctcgtg aaacgatccc tactgaacta
    18841 cttgaaaaaa cagatatcat tataccaaat gaaacagaaa cagaaatcat tacaggaatc
    18901 agagtgacag atcataacag tttggtcgca gcagccgaaa aactccatga attaggtatt
    18961 ggaacagtaa tcattacact aggcagtgcc ggcgctttct atcatacaga aaaagaacac
    19021 ggtatcgtcc ctgcttttaa agtggatgcc gttgacacaa cagccgcagg tgatacattt
    19081 attggcgcat taagcagcac acttcagcca gaccttagta acttgaaaga agcgatcctt
    19141 tatggaaatc tagcctcttc tgttgctgtg cagtcttatg gtgcgcaacc ttctatccct
    19201 taccgcgaag ctttagaaca atcagcagtc taaaattaac aatgagagga agaaacaaac
    19261 atgaaaaaag gaaaagtaat caattcagat atttcacgag taatcgcaca aatggggcac
    19321 tttgataaat taagtatcgg ggatgcaggc atgcctgtcc cctttggtac tgaaaaaata
    19381 gatttggctg tctcaaacgg tcttccaagc tttttatctg ttttagaaaa cgttttagaa
    19441 gaacttgagg tacaatcgat tgaattggcg gaagaaataa agacaatgaa cccggaaata
    19501 ttggaaaaaa tcaaattatt gttaccagat acaccgatta gctttatccc acatgaagaa
    19561 atgaaaaaag agttgaataa ctgtcatgca tttattcgta caggcgaaat gacaccttac
    19621 gccaatatta tattagtgag tggagtggtc ttctaatgga aacgacaatg aatcctgaac
    19681 agatggtact gtccgagcag attcatatgc tgattgaaca gttgggagga aaagacaata
    19741 ttgattctgt ccttccatgt gcgactcggc ttcgtatcaa attgaaaaaa cagccagtag
    19801 ccaatgcaga aaaattaaaa cagcaaatcc cttccattaa gtatttcatt ttactcgata
    19861 gatatgcaga aataatcatg gatcagggat tggctcctgc ttacaaggaa ttaacgcgtc
    19921 aaatcgatca ttcagctcct aaagaaaagc taaatccgat cgaaaaacta tttggtttga
    19981 tatcggaaat ctttacgccg atcattccgc tgtttgccgg ttctggtata ttaaaaggtc
    20041 tggttgtttt agcgactacg attggctggc ttagtgaaac aagcggaact tatcatattt
    20101 tgtctgctgc gtcgaatgcc gtcttttatt ttctgccaat ctttcttgct ttttctgccg
    20161 ccaaaacgtt taaagtgaat ggatatattg cggctgtcat tgctgcagca ctgattgaac
    20221 cgaatctgac ctctttactt tcagacagtg gcaaagcagc tactgatttt ctggggattc
    20281 ctgtagtact gatgcagtat acgtctactg tcatgccggc gattttaggt attttctttt
    20341 attcttttgt tgaaaaattc ctcaaaaaat atattcgcga aaatatgcaa ttagtatttg
    20401 tacctttact ttcattagtt atcacggttc ctttgacgtt aatggtattc gggcctgcgg
    20461 gtgtttacct tggcgaaggt ttagctgccg caattacttg gatgatggat attaacggac
    20521 cgatcagcgg cgcaatcatc gctggtggtt ggaatatttt ggtaatattt ggtatccaat
    20581 gggctgtaaa ccctgtcatg atcagtaata tctctgctta tgggttcgac cgaatcgttc
    20641 cgttgacagg agcagccaat tttggaatgg ctggtgcagc tttaggtgta tttctacgaa
    20701 gtaaacgttc aaaaactcgt tcgatctccg gttctgcttt tgcttcgatt ttacttgcgg
    20761 gagtgaccga accaactgtt tacggaattg cgatcccatt gaaaaaacca tttgttgctg
    20821 cttgcattgg tgcagctgct ggtggagctg taatgggatt tgcccaagtg aaagcgatcg
    20881 cttttgtatt tggtagtctg accacattgc ctgcattcat ttccggtact tttttctggt
    20941 acttagcagg actggctgtt agtctagtcg tagcaatgat tactactctc gttagcgggt
    21001 ttgatgaaga cctaatgtct tatgaatagt taatgaaaaa gactgaagta tgagatgttt
    21061 atcacatatt tcagtctttt tctttttagg atcattattt ttataaattt ttgtgatact
    21121 ttccaaactt tggtataatt tacatatgca taaccatttt tatccccact tgtttatccc
    21181 caaacaaagg tggtctatgc aataaaatga acgtcaatga aaaacgaatg aatgctcgtt
    21241 gcttatttac aaattgtttt atttagaata cacagacaga tttatgaaaa gaaaaagctt
    21301 ttcgacacga ttatctgttc caaaaaatgt ctaatgaatg ctgaataaga cagaaaatca
    21361 atgaaaggaa aaggactagt ttattatgaa aaataaaaaa acgatacgtt tgctagctgc
    21421 tacttggctg attgctccgt ctgttttgtc gaccaatacc gtatttgccg tagataatca
    21481 aacagctgat caaacagtca caacagatac gactacatcc agctcagcta ctagtgaaac
    21541 aacggattct tttacagaag acccaactgg aacaactaac tcaacaacag gatcgaccga
    21601 gccaactgaa attgatccgg ctgatccatc agaaaatgaa aagccagccg agacatattc
    21661 ccttacaatt gctccttctt tgattggtaa gatccaactg actgcttcga cagcggattc
    21721 ccctgaacag caacctttgg atattacgga aagtggaacg gtagaaggtt tattagcgga
    21781 aacgaaggta acttacacta ttacgccagc tgaaggtgaa caaattgctt cagtcgctgt
    21841 taatggtgcc aaagaagtaa attatgatgc aaagaccttt acagtaggta cgaacaattt
    21901 atctattact gcagaatttg aacctgtacc ggatacttca acagagaaac caactgatcc
    21961 tagtgaagaa gtccctagta caccagatcc ggaaacgcca aacaaaccag atgtgggtaa
    22021 taacaacaat aataatggtt caaataattc aaacaataat aacaataatt cgaacagcgg
    22081 gaattcttcg aatcaaacaa acccatctgg atcgaataac aatggaaata ctcagagaga
    22141 accaaaccat ccaaacaatg gttcatcaaa tggcggaacg aacaatgctt catctcgtcc
    22201 aaatacagca aatcaaagcg gtatagagaa tccatcttct gcttcttctg attttgttgt
    22261 aaaatcacca attgatgctg tattgcctac aaatacaacg aatgtccaac aagcaatcgt
    22321 tcgagaagca ttcaagcatc ttggaaaacc atatgtatgg ggagcaaaag gtccaaatac
    22381 ttttgactgt tctggtttga cttattacgt atatatgaaa gcaaccggtc attatatcgg
    22441 tggctggaca ggcgaacagc aatatgcagg tacacagatt ccagtaagtc aggcccaacc
    22501 tggtgatctc gtcttctggg gaccttcttc tggtgttacc caccacgtag gtatctatat
    22561 tgggaacgga caatttatcc atgcgcctca accaggagat aaagttcgag taacttctat
    22621 ttcagatttc acgcctgatt ttgctgtccg agtaaactta gcaggtttac ctgctgcttc
    22681 aggttcgctg acaggcggct catctatttt agatggatta gatggctcgt tccatttcag
    22741 tcaaaaccaa acaactgatc agttcttgaa aaagattgca gatgatgcac aggaaatcgg
    22801 gcaaaaagaa ggaatctacg catcagtcat gatggctcaa gctatccttg aaagtggatc
    22861 tggaaacagc ttattatcaa gcgaaccaaa tcataacctt tttggtatca aaggatcata
    22921 taaaggaagc agcgtgacct tcaatacact tgaacaagat agttccggac aaagctacca
    22981 aatccgcgca caattccgta aatatccttc ttacaaggaa agtttggaag actatgccga
    23041 cttgatcaaa aatggactga ctggcaatcc tgatttctac aaaccaactt ggaagtcgga
    23101 aacaaaagac tacaaagaag caaccaaata tttggaagga cgttatgcta cagatcgcca
    23161 atattcacaa aaattgaacg cgatcatcga agcgtacgac ctgacaaaat atgatgaacc
    23221 gaaaaaagaa gcagcaacaa ctgaagaagc aaacgctgaa atcctcaata tgtctaagcg
    23281 attcgatgtg ccaatcaaat ggacaacaga tggcgtgcca atcacaggat tagaattttc
    23341 aacgataaaa cgcgtcaaca acgcttcttc catcttccgt gtcgcttcaa tctgggattt
    23401 gtggaacaat tttacggcaa gacagatacc agaaacaact actcgtacag ttaaagctgc
    23461 aactgttcct gtactaacct tgttaagttt agacaaatca ttctggcttg atctgccttt
    23521 aaagaaatag aataaattaa aaaggggatg tgacactgtc acactccctt ttt
//
LOCUS       DAPBBG010000044        23571 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPBBG010000044 DAPBBG010000000
VERSION     DAPBBG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.044"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD477_001977"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD477_001977"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDT7634333.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="RD477_001978"
     CDS             complement(1172..1867)
                     /locus_tag="RD477_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDT7634334.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="RD477_001979"
     CDS             complement(1880..2596)
                     /locus_tag="RD477_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDT7634335.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="RD477_001980"
     CDS             2926..3531
                     /locus_tag="RD477_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDT7634336.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="RD477_001981"
     CDS             3856..4179
                     /locus_tag="RD477_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDT7634337.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="RD477_001982"
     CDS             4361..5731
                     /locus_tag="RD477_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDT7634338.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="RD477_001983"
     CDS             complement(5861..7162)
                     /locus_tag="RD477_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDT7634339.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="RD477_001984"
     CDS             complement(7392..9350)
                     /locus_tag="RD477_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDT7634340.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="RD477_001985"
     CDS             complement(9527..9793)
                     /locus_tag="RD477_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7634341.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="RD477_001986"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="RD477_001986"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDT7634342.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="RD477_001987"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="RD477_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDT7634343.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="RD477_001988"
     CDS             13137..13700
                     /locus_tag="RD477_001988"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDT7634344.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="RD477_001989"
     CDS             complement(13953..14474)
                     /locus_tag="RD477_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDT7634345.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="RD477_001990"
     CDS             complement(14477..15319)
                     /locus_tag="RD477_001990"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDT7634346.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="RD477_001991"
     CDS             complement(15312..16037)
                     /locus_tag="RD477_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDT7634347.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="RD477_001992"
     CDS             16213..16509
                     /locus_tag="RD477_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634348.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="RD477_001993"
     CDS             17077..18669
                     /locus_tag="RD477_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7634349.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="RD477_001994"
     CDS             18682..19422
                     /locus_tag="RD477_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7634350.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="RD477_001995"
     CDS             19590..20939
                     /locus_tag="RD477_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7634351.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="RD477_001996"
     CDS             complement(21067..21657)
                     /locus_tag="RD477_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDT7634352.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="RD477_001997"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="RD477_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDT7634353.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DAPBBG010000045        22758 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPBBG010000045 DAPBBG010000000
VERSION     DAPBBG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.045"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..370)
                     /locus_tag="RD477_001998"
     CDS             complement(<1..370)
                     /locus_tag="RD477_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7634354.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIF"
     gene            764..2056
                     /locus_tag="RD477_001999"
     CDS             764..2056
                     /locus_tag="RD477_001999"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDT7634355.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            2071..3840
                     /gene="aspS"
                     /locus_tag="RD477_002000"
     CDS             2071..3840
                     /gene="aspS"
                     /locus_tag="RD477_002000"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDT7634356.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            3949..4824
                     /locus_tag="RD477_002001"
     CDS             3949..4824
                     /locus_tag="RD477_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7634357.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            5090..6973
                     /locus_tag="RD477_002002"
     CDS             5090..6973
                     /locus_tag="RD477_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDT7634358.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(7048..7332)
                     /locus_tag="RD477_002003"
     CDS             complement(7048..7332)
                     /locus_tag="RD477_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDT7634359.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(7322..8056)
                     /locus_tag="RD477_002004"
     CDS             complement(7322..8056)
                     /locus_tag="RD477_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDT7634360.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            8185..8808
                     /locus_tag="RD477_002005"
     CDS             8185..8808
                     /locus_tag="RD477_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDT7634361.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            9000..10121
                     /locus_tag="RD477_002006"
     CDS             9000..10121
                     /locus_tag="RD477_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDT7634362.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            10128..13256
                     /locus_tag="RD477_002007"
     CDS             10128..13256
                     /locus_tag="RD477_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDT7634363.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            13275..16469
                     /locus_tag="RD477_002008"
     CDS             13275..16469
                     /locus_tag="RD477_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDT7634364.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(16615..17322)
                     /locus_tag="RD477_002009"
     CDS             complement(16615..17322)
                     /locus_tag="RD477_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDT7634365.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            18087..18485
                     /gene="spxA"
                     /locus_tag="RD477_002010"
     CDS             18087..18485
                     /gene="spxA"
                     /locus_tag="RD477_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDT7634366.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            18817..19470
                     /locus_tag="RD477_002011"
     CDS             18817..19470
                     /locus_tag="RD477_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDT7634367.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            19696..20694
                     /locus_tag="RD477_002012"
     CDS             19696..20694
                     /locus_tag="RD477_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDT7634368.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            20870..22678
                     /gene="pepF"
                     /locus_tag="RD477_002013"
     CDS             20870..22678
                     /gene="pepF"
                     /locus_tag="RD477_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDT7634369.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         7691 a         3888 c         4788 g         6391 t
ORIGIN      
        1 aaaaaatgtc gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga
       61 gaaactcagg agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag
      121 cgatgacgac acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt
      181 cggaacggcc gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag
      241 ctgtacgaac tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc
      301 caacggcatt tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta
      361 tttctgccat agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga
      421 actaaaacgt ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt
      481 agtaggttat aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac
      541 aagagaggat catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg
      601 agatggacga gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat
      661 ctttcaaaat taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag
      721 ggtgattttt ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc
      781 aaaaggaaca aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac
      841 agcacgttta ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca
      901 ctatgaagtg atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta
      961 tgatttttat gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat
     1021 cgttcgttct tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac
     1081 tttttatatg ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt
     1141 ccatcaaatc ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat
     1201 ggtgatggca cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa
     1261 ttcattaggt gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt
     1321 accttttgaa tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg
     1381 cgtattggac agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt
     1441 agattatcta agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc
     1501 attggctgtt ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca
     1561 tacgattttt gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc
     1621 aggcggacgt tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg
     1681 atttgcaatg ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc
     1741 tgcatttaat gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc
     1801 attgaaagtg gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa
     1861 ccgtaaagca aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt
     1921 gatcggtgaa gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa
     1981 agaaaaaagt tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat
     2041 gactttattc gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta
     2101 gtttccgaac aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga
     2161 gaccttgggg gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc
     2221 tttaatcctg aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac
     2281 gtgatcgaag tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg
     2341 gccactggtg aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc
     2401 ccgccatttc aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt
     2461 tatttggatc tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca
     2521 aaagcgattc gtcattattt agatgataat gactttctcg atatcgaaac accttaccta
     2581 gggaaatcta caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga
     2641 catttctatg cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga
     2701 ttcgaccgtt attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt
     2761 cagcctgaat ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa
     2821 acttacacag agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca
     2881 ttgccatttc ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg
     2941 gatacacgtt ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc
     3001 aaagtcttcc aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc
     3061 gctgcagatc gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc
     3121 ggagctaagg ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct
     3181 aaattcatgg gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac
     3241 ttgctgatgt ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg
     3301 acacgtttag gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta
     3361 actgattggc cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat
     3421 ccattcacga tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc
     3481 tatgcagaag cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt
     3541 attcatactc gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa
     3601 atggaagaac aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc
     3661 ggtattgcat taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt
     3721 gaagtcattg cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt
     3781 atggtttcgc ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag
     3841 ccgataaaaa ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt
     3901 gaaaaacact ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca
     3961 ttcgaaaatt gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg
     4021 ttgatcgcat cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt
     4081 attactggtc tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta
     4141 ccggtatcaa caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa
     4201 atcgggaaga agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc
     4261 catattttac cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg
     4321 gctgtgatgg gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta
     4381 gatatcgtga gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc
     4441 tacttcaatg cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt
     4501 tacagtacgt tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa
     4561 aagaagatgc aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa
     4621 aactgtatgc gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat
     4681 aagcagacag tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca
     4741 atgaaagctt ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt
     4801 cgtttctatg aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta
     4861 gacagacgtt cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact
     4921 gcttttttat ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta
     4981 tgggatgata gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa
     5041 tacccgtata ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca
     5101 aaaaaggtta aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt
     5161 ggtgggtgtt atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt
     5221 tccgattttt ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga
     5281 acaaaatgga aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc
     5341 ggcacttgtt tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct
     5401 gttaaacaat cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca
     5461 aatcaataac ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat
     5521 ctggtcttta gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt
     5581 gatggtattt gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct
     5641 ctccgccatc ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta
     5701 cggtaccgac actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc
     5761 aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg
     5821 aggaatctcg cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt
     5881 catctattac ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac
     5941 agcacacccc ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg
     6001 caaacgaagc tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa
     6061 ggtgactgat attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct
     6121 aatcatcagt gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg
     6181 tcgtacattc caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc
     6241 atgggttgtt ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc
     6301 gaaaaacgct gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa
     6361 attagcagaa gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat
     6421 ccttgataag tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac
     6481 ggcatttggt gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa
     6541 agtagtggta gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa
     6601 attaaaggaa caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc
     6661 attctcggaa gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg
     6721 ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca
     6781 gatgacgaaa aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca
     6841 tagtgactgg ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat
     6901 tcacttagtc agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa
     6961 cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt
     7021 tttttatatt taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg
     7081 tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt
     7141 tgttgcttcg cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt
     7201 ttgaagacga gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag
     7261 atcggttgta tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg
     7321 ttcatttgcc atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag
     7381 tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta
     7441 acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa
     7501 ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag
     7561 taaagcgtcc atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg
     7621 tatgaatctc atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca
     7681 gccctttgaa ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag
     7741 ataagctgtc tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg
     7801 cgatactacg tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg
     7861 cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa
     7921 tcttcccttt tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag
     7981 aaaacacttt ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt
     8041 cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta
     8101 tttttattaa aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac
     8161 cataagaagg gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta
     8221 ctattagcct tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat
     8281 gagaattaca tactagttgc tccccatcgt acttggtggg acccattata tctggcggta
     8341 gctgcagcac ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt
     8401 ctgcgattta tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca
     8461 agcgtcatta aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt
     8521 cccagtggga caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg
     8581 tctaaagcga aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc
     8641 aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa
     8701 acggataaag aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg
     8761 gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt
     8821 atgcgtgggc ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac
     8881 gagggagaga cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg
     8941 tgtcatgtaa tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa
     9001 tgcgtttttt acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga
     9061 ttgaagacca gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag
     9121 aggcaatcgt gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag
     9181 aattatacaa tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta
     9241 tatcaggaaa tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt
     9301 cgaactattt tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg
     9361 tacaattttt ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg
     9421 acaatatccg aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat
     9481 ttgattcaga aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat
     9541 ctgattcgga aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta
     9601 caccgtttga ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata
     9661 atgctcgcta tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag
     9721 gcgtatggat cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc
     9781 tgcatgatat tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc
     9841 agtccgtcaa tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga
     9901 atatgatgaa ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg
     9961 gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat
    10021 tagcagaagc attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat
    10081 ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa
    10141 aattacaact taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga
    10201 cagaagcacc gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg
    10261 atgggatcac cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga
    10321 tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc
    10381 agcagatgag ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa
    10441 acggaacaaa aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag
    10501 aaataaaaca aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc
    10561 tcgatgcgaa acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt
    10621 tggtttcatc aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt
    10681 tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc
    10741 atttagagca agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac
    10801 cagcagcagg gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa
    10861 aacaaatcac ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag
    10921 aaaattccta ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa
    10981 taaacaagca aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga
    11041 ttgggtatta tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa
    11101 aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga
    11161 ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa
    11221 aaatcaaaga agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat
    11281 ccttacgtag taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat
    11341 tgaatgccga gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc
    11401 aagaaaattt actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa
    11461 aatatctaga tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc
    11521 tcgcacagat aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc
    11581 tattgaaaga acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa
    11641 ttgctcgtct tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg
    11701 aacaccccat acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag
    11761 aacaacaact ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa
    11821 gtgaagcaca tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg
    11881 caacgaatat acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat
    11941 ttcctgagat aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag
    12001 atagacgaaa aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag
    12061 cagaaaagct cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc
    12121 agctccagca actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata
    12181 gtaactgttt agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc
    12241 aactagctgt tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga
    12301 cacagcttga tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac
    12361 aagaaagaat agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct
    12421 gtgcatttgc acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc
    12481 atgaagagca attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg
    12541 agacattccc agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg
    12601 aaataaagca agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct
    12661 acgatgaatg tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat
    12721 tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt
    12781 atgtgcttca ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt
    12841 taacgaaaaa tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa
    12901 ccggattaga aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc
    12961 tgtctggcgg tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga
    13021 tccagacaca agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt
    13081 cattagatga agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag
    13141 gaagaatgat cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa
    13201 taagaattga tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag
    13261 ggagattggt aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat
    13321 acttgcaaga aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg
    13381 cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag
    13441 attactgcca atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg
    13501 aactttattt acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag
    13561 aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa
    13621 cacaaaacaa tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg
    13681 agactaatca gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat
    13741 tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca
    13801 cagaaaaaga aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt
    13861 ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat
    13921 tattaggtac gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc
    13981 cagtagaaca agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc
    14041 agataaatga acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat
    14101 tcaaagtgga aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt
    14161 tctttatgca ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc
    14221 aacaaacggt ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag
    14281 tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga
    14341 tcggcgacgt agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat
    14401 ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg
    14461 gcgaagtcat ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg
    14521 tgttacgtga cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc
    14581 agaaaaatga aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt
    14641 tccgcacaga actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc
    14701 gagagttgta tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt
    14761 catggctcga tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc
    14821 tgcaaagctt gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt
    14881 ttgattcaga agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac
    14941 gtacagaaga aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat
    15001 tggaaaaaag caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc
    15061 atccagaaag ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc
    15121 aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag
    15181 ccgatgaaat gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag
    15241 aagaacggaa aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc
    15301 aggcagaggt gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa
    15361 aagaacgtga agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct
    15421 taagacagga tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg
    15481 cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac
    15541 ctcagcggtt tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat
    15601 tgtttgcaac gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg
    15661 cagaagaaat tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt
    15721 tgaaagactt gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa
    15781 aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg
    15841 aagcattcaa gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt
    15901 gctgcgatcc tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag
    15961 taaaagatct actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat
    16021 ttacagggat gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt
    16081 atttaagagg cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg
    16141 gcgaaaaaga tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg
    16201 gagcagatac ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg
    16261 ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg
    16321 aaggaacgat tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa
    16381 aagtgatcca aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga
    16441 ttttaagtgt aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa
    16501 gaaagccttt ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga
    16561 ttccatttta tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac
    16621 aaagattgtc ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat
    16681 tggagtctgt tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact
    16741 ttttgcacct agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag
    16801 catatgaaat tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt
    16861 tgcttgagaa atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag
    16921 tttttttgtc tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc
    16981 cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc
    17041 ctcgtaaatt ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc
    17101 ttttagtgat tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat
    17161 tacttttggg aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc
    17221 tgcatctgaa acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc
    17281 atacatcgta tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg
    17341 gttttcattg attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa
    17401 gctgataaat aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta
    17461 tatttcagtt tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta
    17521 atcattatgg tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag
    17581 atttattttt ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat
    17641 ttatttgttt atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata
    17701 acagagaaaa taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac
    17761 tattaaagaa ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga
    17821 taaaataata gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt
    17881 gaaaactcta atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac
    17941 cttagataac tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg
    18001 attatgtata atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt
    18061 cttatcacga aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt
    18121 cctgcagaaa ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata
    18181 ttttttcaga accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg
    18241 gaacagaaga aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag
    18301 atgatctgcc gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc
    18361 gtccaatcat gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc
    18421 ggttcctacc gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat
    18481 tatagaagga taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt
    18541 tttgttttta caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat
    18601 tgcacgaaaa atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc
    18661 aaaaatgtta tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa
    18721 attcgcttaa cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc
    18781 tatattaaac attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa
    18841 aatacgatcc gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg
    18901 gatttacttg gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt
    18961 gatatcgaag aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga
    19021 gacggattag aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa
    19081 gattctactg ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc
    19141 gatccggagg aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt
    19201 gcttttgaag attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct
    19261 tggactgatt tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa
    19321 aatctcaacc aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag
    19381 agcgatcgta caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct
    19441 atcgagcgaa cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa
    19501 aattgtgtcg cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca
    19561 agcccaacca aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat
    19621 gcagaatagc tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag
    19681 ttcagaggga gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa
    19741 catgcagaaa aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa
    19801 aagggaaaga aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt
    19861 agtgaaagag aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga
    19921 cagtcagtag aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat
    19981 attttgttgg cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt
    20041 attgaaagaa cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat
    20101 tcttttcttg gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga
    20161 aaaagaggag tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat
    20221 catattacag agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct
    20281 agtagggacc ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa
    20341 atagaaaaag tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa
    20401 aagaatacag tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta
    20461 tcatcgtgga tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt
    20521 tatcgaatgt tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg
    20581 ctttgccaaa taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg
    20641 gtctatcagc agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta
    20701 taaaaatagg ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga
    20761 tggaagcaga cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact
    20821 atgatctatt ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa
    20881 gcagttgcca aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt
    20941 ttcaagcgac caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc
    21001 tgaaaaacac aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt
    21061 cgtattgagg gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga
    21121 tcaagacact ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc
    21181 gaaagttagt gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca
    21241 gatttggcaa tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca
    21301 aatagtagat aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc
    21361 aggtgaaatc tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt
    21421 ttttccaacg attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg
    21481 tcagttgcta gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag
    21541 tgtttacgaa caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca
    21601 taattttaaa gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa
    21661 tcatattcct gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt
    21721 gttacatcga tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta
    21781 tgatctttat acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa
    21841 agaaaaagct ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa
    21901 agcattctct gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta
    21961 ttcttcggga agctatgaca caaatccata tattttattg aattggcatg atacgctgga
    22021 tcagctattt acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc
    22081 gaaccagcct tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac
    22141 gaatgaaaat atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc
    22201 ttatgtactc aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt
    22261 cgctgaattc gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga
    22321 atacctaagt gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga
    22381 agacccagaa attaaattcg aatggtcacg gataccgcat ttttattata attactatgt
    22441 tttccaatac tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca
    22501 ggaaccagag gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc
    22561 tgtggaagta atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc
    22621 aatgtcgatg tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg
    22681 ctttctattt ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg
    22741 gagtcgacgt attttttt
//
LOCUS       DAPBBG010000046        22504 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPBBG010000046 DAPBBG010000000
VERSION     DAPBBG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.046"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(80..1456)
                     /locus_tag="RD477_002014"
     CDS             complement(80..1456)
                     /locus_tag="RD477_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDT7634370.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1584..2702)
                     /locus_tag="RD477_002015"
     CDS             complement(1584..2702)
                     /locus_tag="RD477_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7634371.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2844..3124
                     /locus_tag="RD477_002016"
                     /pseudo
     CDS             2844..3124
                     /locus_tag="RD477_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3202..3735)
                     /locus_tag="RD477_002017"
     CDS             complement(3202..3735)
                     /locus_tag="RD477_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDT7634372.1"
                     /translation="MRVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3829..5007)
                     /gene="mutY"
                     /locus_tag="RD477_002018"
     CDS             complement(3829..5007)
                     /gene="mutY"
                     /locus_tag="RD477_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDT7634373.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5000..5797)
                     /gene="recX"
                     /locus_tag="RD477_002019"
     CDS             complement(5000..5797)
                     /gene="recX"
                     /locus_tag="RD477_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDT7634374.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            5904..7286
                     /gene="rlmD"
                     /locus_tag="RD477_002020"
     CDS             5904..7286
                     /gene="rlmD"
                     /locus_tag="RD477_002020"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDT7634375.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7460..8038)
                     /locus_tag="RD477_002021"
     CDS             complement(7460..8038)
                     /locus_tag="RD477_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDT7634376.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8360..8830
                     /locus_tag="RD477_002022"
     CDS             8360..8830
                     /locus_tag="RD477_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDT7634377.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(8938..10164)
                     /locus_tag="RD477_002023"
     CDS             complement(8938..10164)
                     /locus_tag="RD477_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7634378.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYIPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(10918..11118)
                     /locus_tag="RD477_002024"
     CDS             complement(10918..11118)
                     /locus_tag="RD477_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7634379.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(11252..11860)
                     /locus_tag="RD477_002025"
     CDS             complement(11252..11860)
                     /locus_tag="RD477_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDT7634380.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(11945..12415)
                     /locus_tag="RD477_002026"
     CDS             complement(11945..12415)
                     /locus_tag="RD477_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDT7634381.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(12463..13377)
                     /locus_tag="RD477_002027"
     CDS             complement(12463..13377)
                     /locus_tag="RD477_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7634382.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            13523..14326
                     /locus_tag="RD477_002028"
     CDS             13523..14326
                     /locus_tag="RD477_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDT7634383.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            14504..15451
                     /locus_tag="RD477_002029"
     CDS             14504..15451
                     /locus_tag="RD477_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDT7634384.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(15519..15812)
                     /locus_tag="RD477_002030"
     CDS             complement(15519..15812)
                     /locus_tag="RD477_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDT7634385.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(15840..16505)
                     /locus_tag="RD477_002031"
     CDS             complement(15840..16505)
                     /locus_tag="RD477_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDT7634386.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(16645..17277)
                     /locus_tag="RD477_002032"
     CDS             complement(16645..17277)
                     /locus_tag="RD477_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634387.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(17284..18351)
                     /locus_tag="RD477_002033"
     CDS             complement(17284..18351)
                     /locus_tag="RD477_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7634388.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(18348..19079)
                     /locus_tag="RD477_002034"
     CDS             complement(18348..19079)
                     /locus_tag="RD477_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDT7634389.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(19247..19726)
                     /gene="greA"
                     /locus_tag="RD477_002035"
     CDS             complement(19247..19726)
                     /gene="greA"
                     /locus_tag="RD477_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDT7634390.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(19862..21037)
                     /gene="mltG"
                     /locus_tag="RD477_002036"
     CDS             complement(19862..21037)
                     /gene="mltG"
                     /locus_tag="RD477_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDT7634391.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            21202..22362
                     /locus_tag="RD477_002037"
     CDS             21202..22362
                     /locus_tag="RD477_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDT7634392.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         6788 a         4554 c         3614 g         7548 t
ORIGIN      
        1 tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag caacacgtct
       61 ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct tttctgaagt
      121 atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa atgacacgga
      181 ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta tcgacatcaa
      241 acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa taaccagcca
      301 tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca
      361 gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga ttctcaactt
      421 catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct agtgttgcta
      481 acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc gtctttttca
      541 tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc tcctgcatga
      601 ttttcttgat atctaaatta tcaaaaccga atttatgggc agcttttagt aaattctttg
      661 tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt cttgaatagt
      721 tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca atcataaatg
      781 catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa aaaacccctt
      841 ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata taacgcattt
      901 catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata cgcgccaata
      961 aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca atcaagcgta
     1021 tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata cgtttagggt
     1081 acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg atatttgcaa
     1141 tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat aaagtattag
     1201 ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt gtcgatagga
     1261 aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct ccttcatcag
     1321 ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa gctgctaaaa
     1381 aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc gactgactct
     1441 cagggtcagc attcataatc gcattctctc cttataaaat taatttttag tttacgatag
     1501 aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt tctttgcaat
     1561 aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt gttttcattt
     1621 tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca gattgcgtaa
     1681 aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac tagaagaacg
     1741 ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg ataaatgata
     1801 ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc ttttaccgga
     1861 tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc taaatatgga
     1921 attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac accaattacc
     1981 atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg tccgctaatg
     2041 taattagcca atgttttatt cagttgcccc aataactcca caatttggtt tcttcttttt
     2101 tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa catatagaac
     2161 aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc aacgatcgaa
     2221 cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta agaaatgtct
     2281 aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc ggctacctct
     2341 tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt
     2401 tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc aactaaaagg
     2461 agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa cagcttgaca
     2521 ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa taacgtggaa
     2581 aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat gatcaatgca
     2641 gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag tttttcaaac
     2701 aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa attcttttat
     2761 tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc cagtatacta
     2821 tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca atgagcaata
     2881 tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag ggtatgcctt
     2941 tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg tactcagata
     3001 aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa atgaaattac
     3061 agcggatggc gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt
     3121 ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg
     3181 gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat
     3241 atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt
     3301 cacattttct tttaggatga agtcaatatc gttcggataa ttcatcattt tgctgtgcat
     3361 ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa
     3421 gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca
     3481 ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg
     3541 aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa
     3601 tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg
     3661 ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc
     3721 ttttggaact ctcatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt
     3781 tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt
     3841 cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac
     3901 gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg
     3961 ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa
     4021 aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa
     4081 tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt
     4141 ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt
     4201 ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg
     4261 attttgaaga gccgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac
     4321 aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt
     4381 ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt
     4441 gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct
     4501 gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac
     4561 gttgccgtca accgcgggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt
     4621 atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc
     4681 aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc
     4741 aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt
     4801 aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg
     4861 aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt
     4921 tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc
     4981 tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga
     5041 tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt
     5101 taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat
     5161 attctatttc ttgatctgct tcttgcggaa gatcagccat cgcttgttgg atcacttctt
     5221 gagaaaaacc tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt
     5281 ttccgcgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt
     5341 caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca
     5401 tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac
     5461 tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat
     5521 ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc
     5581 gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct
     5641 tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta
     5701 ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag
     5761 ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat
     5821 tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag
     5881 aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc
     5941 ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga
     6001 tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa
     6061 ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag
     6121 tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc
     6181 aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag
     6241 caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata
     6301 gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt
     6361 tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc
     6421 gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct
     6481 atgatgaacg gacaaacagt ggaatcttcc gcacgatcat ggttagaatc ggtgttaaaa
     6541 ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa
     6601 tgatctgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg
     6661 cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca
     6721 ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca
     6781 atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta
     6841 atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac
     6901 aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa
     6961 atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc
     7021 tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta
     7081 cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt
     7141 atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca
     7201 agattgaata tttacagtct gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg
     7261 tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc
     7321 aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac cgtacttgtg
     7381 accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg
     7441 tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg
     7501 tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa
     7561 gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga
     7621 tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt
     7681 agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc
     7741 ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc
     7801 cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac
     7861 tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc
     7921 ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa
     7981 aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt
     8041 agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt
     8101 atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa
     8161 cagaagtaga cgagaaaagt acaaatagaa aacatgagaa ccaaatcata tgatcttttt
     8221 caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat catcaatttt
     8281 tttcttactt cacaaactaa atacaggtct tgctgtgagc cttcatggta aactactatt
     8341 atagaaagga gttagtaaaa tgaagatgat aaaagatatt cgtttttggg tttgtgttat
     8401 aggaattgtc atattaggct ttttatcagg gttgcttagc ggaaatcccg gagaatatta
     8461 ctattctttg caacttccac catttgcacc acctagctgg atatttgggc cgatgtggac
     8521 cttactgtat atattgatgg gtatttctct ttatttgtta cttaatcaca acaacaaaaa
     8581 acagcgaaac aatcttgtcg gtctttttgt gattcagttc atttttaatt ttatatggtc
     8641 tgctctattt tttaatttaa gaaatatttt cattgcagca atcgatatta cacttttagt
     8701 tattttcttg tcagtgctta tgtatcagtt gtggctacat catcgcctgg caatgtggct
     8761 catgattcct tattatttat gggtactatt cgcaactcta ttgaattatt caatttattt
     8821 tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa agtgccacct
     8881 aagttagatt ttttgtctaa tttaggtggc actctttatt aaccaaacta tttttttcta
     8941 gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat tttgttcctg
     9001 tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact gacttgtatc
     9061 ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt tttctgaata
     9121 ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg aatcgccatc
     9181 ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag gtaatgtatc
     9241 gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa gatcattttt
     9301 tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca actggtcttg
     9361 tttgattgtt tcaaacgcag ggatgtagtt cgtcgctaca tcaagcatgt catcccaatc
     9421 taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat tgcttacgcc
     9481 atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac gacgttgtcg
     9541 accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac ctttttctcc
     9601 attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt caattttatt
     9661 attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca aattgatcgt
     9721 tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg ccatttcgac
     9781 accaccaaat gcataggcat gatttaattt atctaccgtt ccataaccta cgatattcgt
     9841 ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat taactgttac
     9901 taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg tatctaaacc
     9961 taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag aacgtttgga
    10021 ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt ctcccataaa
    10081 tctgattccg tatgctgata atccggcaat cagcaaaaca aataccccta acaaggttaa
    10141 tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta aaatattgtc
    10201 tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt taaaaaacat
    10261 ttttgtgtaa cgattactat tttttaaaat ttctcaaaat tcttaactat cataccatac
    10321 cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttacg
    10381 tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga
    10441 tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga
    10501 tttcacgaaa atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt
    10561 atttccagct cactgcatgg ctcttaaaat gactcgaact agtgatttca ttaccatcat
    10621 cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga
    10681 attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc
    10741 caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa
    10801 ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc taaaaacttc
    10861 ataaatagac aaaaagacac gactctctaa gaagtcgtgt ctttattcaa caaattatta
    10921 taatttagtc acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg tgaattctac
    10981 ttcttgacct tcttctaggc tcttgaagcc gtctccttga attgctgaga aatgaacaaa
    11041 tacgtcatct ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt tgaaccattt
    11101 aaccgttccg tgttccataa atgtatagcc tccaaaaaag ttcttgcaag taaccttgca
    11161 agaacattat acccaattta gaaaacgcta gcaagctttc gctcttttat tttccaaatt
    11221 atttttaaga ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa cgtttgttta
    11281 cttcatccca attaataaca ttccagaatg ctgcaatata atctggtcgg acatttttat
    11341 atttcaaata ataagcatgt tcccaaacgt ccaaacctag aattggtgtc tttccttcca
    11401 tcaatggaga atcttgattt gcagtagagg taatagctaa ttttccattt tccattacaa
    11461 gccaagccca tccagaacca aatcgtccag cggctgcttt tttgaattct tctttaaaag
    11521 aagaaaaatc accaaaagct tcattaatcg cttcttttat ttctcctgta ggttcgccac
    11581 cagcatttgg tgccatgatt tcccagaaaa atgaatggtt agcatgtccg ccaccattgt
    11641 tacgtacagc tgtcttgata tctgttggaa tagcgtccat atcagataat aattcttcta
    11701 ttgttttttc gcctaattct gggtatttct caattgctgc atttaaattc gtcacataag
    11761 tattgtgatg cttatcatga tgcagatgca tcgtttcttc gtcaatataa ggttccaatg
    11821 cgtcatatgc ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc ctcctcaatg
    11881 ttttctttat aatgtctaca ttacaaagag taacaaagag aaggaaattc ttcaattgtt
    11941 atgcctagtc gtctttttca ctttgaccta cttcttcttc aaaaacctca gtttctttaa
    12001 aaataaacaa cttgctgaaa atatagttcg ctaggataac aataatttga gtgatgattt
    12061 tcgccacata ctcattgctg ttcataacat tgatcaacaa taccattgcc cccatgtcca
    12121 atccgtaaga aaggatacgg tagaagaaga acttaccgca ttcaataagt gcatgtgtaa
    12181 aattcggtga ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca aacaatacag
    12241 aacaaaacca cgaaatagaa ttggataagg ccatacttaa atcaaaatat tggcgtgcga
    12301 gagcaaaact aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga tagactagta
    12361 tctcccaata tccttttgct tctaaatatc cttttaactg tcgatactgc ttcatgagcc
    12421 aacctacctt attaaaaatt cttatttatc atactctata tttcaataac tttcatcttt
    12481 atttaaaaaa attaattttt tttgatcctt tgcctttgtc tctgcttgga tcgtcacatg
    12541 tgtaatacca tattttaatt ttaataaaga aattatacga ggatatatct tttcaatctc
    12601 gcttagtagc tgatcttcaa aatcaatatg tccgtcgaaa ataattatct tttcgctata
    12661 ctgccaaata tggacatgat gaacattctt taccccatcg attgcttcaa tttcttgttc
    12721 cattttttca tagtcaatag caggtgcact ttgtatcaaa atagcggcag cttttttgat
    12781 caccattatc gcttctatca atatatatat cgaaatgatc agtgtaataa ctggatcgat
    12841 ccagtagata tcgaaaaaac ggatcaaaat tgctccgata atcacactta ccgaagacag
    12901 tgtatcactt aacaaatgca aataactaga ttttatattc aaactttcat ctgcttcttg
    12961 tcgtaataat aatactgaga acaaattcgc gagcaatcca ataactgcta ctgttagcat
    13021 caaatgacta tttatctttt ctggtgaagt gaaccgcttg aatgcttcaa cggttaaaaa
    13081 caaggataat atgatcaata tgaatgagtt gatgaaagca ctcaatattt ctaaacgctg
    13141 gtagccatac gtacgttttt tatttgcttc ttttcgggcc attttttgtg ccccgtaaga
    13201 aaaaataatt gccaatgtat cactaaggtt atggaaagca tctgaaacaa gagacaaact
    13261 ccctgacata atccctccaa taaattctga taaagtgatc accgcattca aaaccactac
    13321 tccaaatagt ttttttgatg atctctgtgg ctgtgtaggc actattcctt cctccatttt
    13381 atttatattg gatagtcttt agacaagaat aacatttttt tgatatttca ccaatcattc
    13441 agatgtttcc ctaaagcact tgtatttcac ttgatacttc gctagaattg caagtgatga
    13501 cactactaga tgaggagtac ttatgacaac aaaacaatta attatcagtt cctttatacg
    13561 attcaaagac ctaaaggagg cgagaaatgt cccattttgg aaagccattg tctatctttt
    13621 agttttgagt ataattatgg ctttgccaat ctcttttcaa gtttttcaag tcttaaataa
    13681 cattcaattg gatgggcaaa agattgctac tcgcattcct gattttacaa tccaaaacgg
    13741 gcagcttcaa accgaagaaa aagaaggatt catttaccaa acaaactcca ttatcttcac
    13801 atttgatcca gagggaaaac gaacagaaca ggacatctct actgatttga tggggaattt
    13861 tgttagtgtc gggatgttaa aaaacaaact tatcatagct cttcctaata caggcacgac
    13921 ttctgctttg ttgaacaata accaattaga acttccctac accaatgaat ctttaaaaaa
    13981 cttaacaggt aagcagctcc gttctttttt aagtgaagca tctataccac tttggataaa
    14041 agtcctgaca tttatatttt ctatttatcc tagttttctg aatctgctga tcactttgct
    14101 atttaccaac attgctgctt ttctctatgc acgctttcga ctaacaaaag ctacttttct
    14161 tgattgttta aaaacattga tctattcaat ttcattgcct actataattg caacaatctt
    14221 gatggtcttt cttccttctt ttgacactag cgccttcatt gcttttgcag gaatctttat
    14281 ttttgctcaa gctgtcaaag gatgggcaaa aatatcgatc aattaagaaa taaagacaac
    14341 tcgttatttc tacttcaata gaccctttta gaggttgtga catttttttg tcacagcccc
    14401 ttttttattt gaaaaatatt tctttctcaa aaaacaagca aggcgcttca aagttcttct
    14461 tgtatgcgct acacttagag taaagaaagg cgggaaatta actatgtcag aaagaatcat
    14521 ttatgcagat cgtccatttc gtgatgaatt tatcgaagaa atccatacta tagcgcctgg
    14581 ttatatcttt aaaacagaat tacaaccaga ggatctacaa aatgtagaga tcagtttggg
    14641 ctggaactca gactaccaag aaaaactatt agcctcttct accttgaaat gggtccaatc
    14701 tatctcagct ggtgttgatc agctacccct tgagactttt tcaaagaaac atattttact
    14761 ttcaaatggt agtggcatcc acagtgaatc gattacggag catgtcttag gaatcatcct
    14821 tgggtattct cgtggtcttt ttcaagcaca aaaggcacag agtgacatga aatggttagg
    14881 ttcatcggtc cattaccaat ctatccaagg gaaaaaaata ctaatcattg gaactggcca
    14941 tattggaaaa atgattgcaa aaaaatgcaa agcattagac atgaactgct atggaatcaa
    15001 tacatccggt catccagcag aaggaatgga aaagactttt tctttaaatc aactaaagga
    15061 agttcttcct gaagtagatt tcgtagtcaa tatcttacct ctaacggagg aaacaactgg
    15121 gctctttgat cagcatatgt ttgaacactt tgatccaaaa tctgtattca tcaatgttgg
    15181 ccgtggtgct tctgttaaga cacaagatct ggtgcaggct ttaaataacc agcaactttc
    15241 ctttgcagca ttagacgttt ttgaggaaga gcctcttcca caagatcatc ctctttggaa
    15301 aatggacaat gtactgatta cttcccacat tgcaggtttg acacctgatt ttcaaaagaa
    15361 attaatggct atttttttaa ccaatctgaa aagctacttt gccacccatg agcttcaaac
    15421 caatcaagta aaattgtctg caggatatta atagctgaaa acaatatttt caaaagaaaa
    15481 aagagaaggt gacaaaaatt gtgtcaccct ctcttttttt attgtttttt aaagtgatag
    15541 gttggctcct ccaggttggg atcataatca acaagtaaat catatccttt aaagaaccat
    15601 tcatctgctt cctcgataaa aaaaagtgtc ccatccattt ccactttctt tacaggatgt
    15661 tctggctgtt caactgacat tccaatcgaa aaaccatcat gaacctctgt tttgccgtag
    15721 acttttccaa aaaaacgtat tccgtaagtt tctggcagat caatttctga gtcaaaccat
    15781 ttttgtgctt ttggcgttac cgttaatttc atcccaacaa cctactttct cttttctttc
    15841 tattgattca tcttatcgtc tagaatatcc acatcgcctg tctctccaat aaccagtaga
    15901 ttatcatttt cacgaacata ttcatctgct gcaggagaag cgataaccgt ttgatctact
    15961 cttcgtatgg cgactactgt taagtcaaaa cgttgcctga aatttaattc agctaatgtt
    16021 ttgttgaaaa atttaggatt ggagactcgg atttctgcta aagaatattt atctgacagc
    16081 tctaagtagt ctagaatatt ctttgatact aagttatgtg caatccgtat ccccatgtct
    16141 cgctcagggt gtacgacacg atctgctccg atcttctcta gtacacgggc atggtactca
    16201 ttttgggctt ttgctaaaat attcggtacg cccatttcct ttaccatcag agtaactaag
    16261 atgctggctt ggatatcttc tccaatagcg acaattacat gatcgaaatt tcttatacct
    16321 aatgaacgaa gtgtcatctc atcctgtgca tttgccacaa cagcatgggt ggctatattc
    16381 atatattcat tgactcgatc ttcattactg tcaatagcca gtacttcttg tccagcttcg
    16441 atcagtgtac ggcagatgct tccgccaaat cgtccaaggc ctacaattgc aaaattttgt
    16501 ttcaccattt tcctccttta gtatcttctt ttctcagtat aacaaagcct ttccaaaaac
    16561 aaaagcaagc atcattattg taaaaaagcc aagaaaaata atagttattt tctcagcttt
    16621 tgattatcct ctttatcatc gtttttattt caccaaacca tgtttaaacg catagatagc
    16681 tgcttgagtt cggtcttcta cctctaactt agctaatata ttcgacacat gcgttttgac
    16741 tgttttcagt gtgataaaca attcatccgc gatttcctga ttacttttcc cttcagaaat
    16801 cagcatcaaa atttcatttt ctcgattcgt taattcttca tgcaatactg gatcatttcg
    16861 ccgactcatt ttttccatca tttttgttgt cacttcaggt tctagtactc gctcgccgcg
    16921 ttgcgtagcc cttatagcat cagctatttc atgtgctgta gacgtcttca acaaatatcc
    16981 tgctgctcct gcttcaattg caggataaac tttttcatca tcaataaaac ttgtgacgat
    17041 aatgattttg gcttttggcc aatcttttaa gattgctttt gttgattcga tgccatccat
    17101 ttcttccatg actagatcca ttaaaatgac atccggtcga agtgccatcg ccttttcata
    17161 gccttgacgt ccattttcag cttctccaat cacttcaatg tcttcttgga tagataaata
    17221 agaagagacg cctaagcgta ccatttcatg gtcatctact aataaaactt ttatcatctt
    17281 tatctatgcc tccttcatca aagggacctt ggtctccacg ctcgtacctt gccctttaaa
    17341 actaatgatt tttactgttc caccaatacc agctactcgt tctttgatat tgttcaatcc
    17401 ataacttccc gttttggttt cattcatatt aaatcctgtc ccgtcgtcaa tgatacgtaa
    17461 taaaagattg ttatctattt tatgcaaata gacttctaat tcattagcct tagcatgtct
    17521 taatgtgttt gataacaatt cttgaacgat tcggaacaag tgatcctcaa tggaactagt
    17581 taattttaca tcttctacct cccattttag cgaaatctgg attttattct gcaattcctt
    17641 caataactgc tcaatgcctt gttttaaact tttttcttct aaattgactg gacgcagatg
    17701 aagaagcagc gcacgcatct cagactggga agcgttgatg atctctgcca ccattttcaa
    17761 ctgcttacgg aacatttctg gtgtctcact tttttctgct tgctccgtca atgccgacat
    17821 catcatcatc gctgcaaata attgctgtga aactgaatca tgaagctcac gagctaatcg
    17881 atgcctttct aattttaaaa tctcttcttt ggtttgtcca tctacatatt gaggacggct
    17941 agtcataatc tgtaattcac tggatacttc gatcattttt tcttttattt tagtaatttc
    18001 catatcaata tctttgatgt acagatcatc cgacgcgctt gggatcggct gatgcaaaag
    18061 tttggattca taattgccag aagaaagtgc acgaagcttt tcttcgattt tcccgtattt
    18121 tgccttttga ataagtgata acaatagaaa tacgatcaat cctacaccta atgaaattgc
    18181 catcaaatgg aagataagcg gaacgtataa taaacgcgca cgcagcaatt ctccccacca
    18241 atgactttgg ttactggcat aaaaataagt gaaaagtgaa aatagaataa taaggaaagc
    18301 cgcaatcccc gaataaacag ctagcattgc tttggatatt cttcccatca tattcgtatc
    18361 acctcgacat ctccaaccaa agtattggta atgatcttca atctgcgagg attttcatca
    18421 taatcactgc tgcagatttt gatctgttcg ttttttaagg aaaactgctc ttcttcaaac
    18481 aagacatttc ctactaatgt cgcatgctct aaacgaatag ccactcccag gggtactaag
    18541 atccgtgttc tgcctatccc ttttcttaca atcacaatat tatcatcttt tggcaaaagt
    18601 gtattgccca aatcgatgat agtatcacca gaaatgatgg caatgttgat atcatcccat
    18661 tcatagacgt cgtttccaat acgttggtta ccaaataatt gctgtctttt acgctcatta
    18721 ttatgtgttt ttaattgctc tgtttggacc atcatgatct gtttttttcg ccaaaaagca
    18781 ttttttgtca aatctattcc tgaaatctct acacctttca gcccgataaa taaaacggcc
    18841 aatacaacca tcatccataa agcaggactg ttgactaagc taaaaaaaat gaccaagctc
    18901 cctaagatca attgaaaatt ttgaaattta gtacgaggat atttgcgcat agctagatat
    18961 atattgaaaa taccgaatat tactaaaagc aaaagtccag tattgtttac gatttgccaa
    19021 acagcaaata acagcagcag tgcttcgact accacaaaaa aacgccaaga actattcatc
    19081 gctttttctc cctcctgaca cattctcatt tcatcatacc tatttttttg actgttggct
    19141 ataggacttt agatgtaaga tcgttcagtg aaacctgcaa tactcaaaga gaaaagatct
    19201 agagtgcgtc atcagtaaga tgaactctct agatctttca gtcagactac gcttgttcta
    19261 ctttgacgat tttaactaac atatcgccac ctggtgtagc aatggttact tcatcatttt
    19321 tacgtttgcc aatcaatgct tgcgcgatag gagaatcatt ggaaatcttt ccagaaaacg
    19381 ggtctgcttc agcacttccg acaatcgtgt actcttcttc atctccatca ggcaactcga
    19441 tgaacgtaac tgttttaccg atcgatacct catctgcatc tacgccgtca ttatcgatga
    19501 tttgtgcaaa acggatcata ttttcaattg ttgtgattct tccttcaaca aatgcttgtt
    19561 catctttagc tgattcgtat tcggagtttt cggaaagatc tccgaaaccg cgagcgattt
    19621 tgattcgttc aatgatttct ccgcgtttaa ctgtttttag ttcttctaat tctttttcta
    19681 atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt aacgtctcct
    19741 tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa aaaagattac
    19801 cacgcttttc ttaaaatgta aatgtttttc taaaaatgat caatgactag cccctgtttt
    19861 tctaagaatt atttacatat ttttgcacta gttccatatg ttcttcatac gttttagcga
    19921 aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt tctgctggtt
    19981 ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggaccaggc ccatagccgg
    20041 tattggtata caaattgtat ggtgaatcta cagctgtatc ttcatacgtc accaattctt
    20101 tatgttctcc taatgcatat aaaacagaga tatctgactg aagtggcata tttgccttga
    20161 ttcggttaaa gaagacttgt gcaatatttt tacgatcatt ttcttttacg ccttcttttt
    20221 caactagaga agccaaagtt aaaacttctt gtacagtcaa atttttctgt ttgatagctg
    20281 aataatagtt ttgcatgacc gtatttgttt tgttcaccat ctcaatcaca agatccttaa
    20341 gcgaagtact cgtgtagtaa tcataagtag ctgggaataa gtagccttcc aaacggtatt
    20401 ttgtgttctc agctttagag gcactttcca acaattctgg gaatttttga tacaactcgt
    20461 taaagaaagt ctcatcattt actaaatcta aaaattcttt cttgtcttta ccagttacct
    20521 tcgctacccg ctcagcaatc tgatcgatgt cgtagccttc tggaatggca attttcccat
    20581 ctgcaacttt tgttggctca gaagtgccgc cttcttgcag ctgtttgcca atctcgtcaa
    20641 gagtcatatt aggcgctaac tgataatagc cagcttgaaa gccagttaaa tttttaaatt
    20701 ttgtataata attgaaaaca atcccgcttt taataatatt gtccttctcc aaaatctcac
    20761 caatctgctt gttagaagag ccactcggga tctccacttg gactaattgg tcatctgatt
    20821 tatctaaagg cttcaacccg ctatctacat aacgatacac ggtaaatcca agaaatccgc
    20881 caataattaa taatgtaaga gcaatcacta aaatgatctt cctcacaatc ttatcttctt
    20941 ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta ctggcttctt
    21001 ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac aactcctcct tactatcatc
    21061 ttcgtaactc tgtttattat acatgaaatt tctactgccg tgaattattt taccaaatat
    21121 tatgtcaaaa ttgtaagtgg caaatactct ttttgaggat tacaatgaaa aatagttaaa
    21181 aatggataga aaggaattaa catgtcaaaa ggaaaacatc gaaaaaaaca aaaaatttct
    21241 atttcttttc ttgtaatttt attattgtct gtcagcttct tttcaacagg catcattata
    21301 ggaggtcaat taacaaaaat acctcattcc caaacttata aagcggagcc aacacaactt
    21361 acttccaatt taacgcccga tgagcagagg cttgctagca aaattgatca attactacaa
    21421 aaatcagatt atatcggttc gatttatgtt cgcaaaaata atcgcgtact attagaaaaa
    21481 ggatatggat atgcaaatct gcgcgtaaaa gaatcgaatt caccttctat ttactatcaa
    21541 atcggttcga ttcaaaaagc aatgactgct ttactgatac taaaacaaat ccaagctggt
    21601 aagttaactt ttgacacaaa gttagcagaa ttttatcctc agattcctgg aagtcagcaa
    21661 atcacaatca aaaatatgct ttatatgcgc tctggtcttc atcgaactgc ttctcccgac
    21721 aaacctatgt cggatagtga aatcattcag ttcgcccttc atcatttgaa acttactgat
    21781 tatgatacgt atcattatga accattgaat tatacattgc ttacagggat attgatcaaa
    21841 ctaacgaatc agccttatga aactcttcta aaaaaagaaa tcattcgtcc actccatcta
    21901 gaacatacag atttttatga caatgtaaaa aattcgcctc aacatgctgt atcttaccag
    21961 atgtctgcaa ctgacgatta ttcaaaagca ttgatcgagc ctgaaaccga catccgaaat
    22021 gaattaggta caggaaacat aagtatgtct gtttatgact tgaacaaatt ctttaccagt
    22081 gttctttctg gcggtatcat accaaaagaa ctacttttct cattgtggca aaataatggt
    22141 gataaacatc cttacagcgg cggtgtttac agtggaaacg attatatcct tgctcagggg
    22201 aatatcaatc gattccatgc agtagtggcg atgaaaaaag acactaaaga tgcaatcgtc
    22261 atggaaagta atatccaatc ggataagaac atcaaattgc cggccactga tttaagaaat
    22321 cagatttatg aactgatgga aggtgttcac ttatcaaatt aaattttgac ataatattag
    22381 ttgtcatctc tgtatagtca atttagttag tactctatct gaaaagtaga aataaattaa
    22441 caaatattat ctgtccttat tatttgttaa tttatttctt attttactta aataattttt
    22501 ggta
//
LOCUS       DAPBBG010000047        21918 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPBBG010000047 DAPBBG010000000
VERSION     DAPBBG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.047"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            140..460
                     /locus_tag="RD477_002038"
     CDS             140..460
                     /locus_tag="RD477_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634393.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="RD477_002039"
     CDS             912..1112
                     /locus_tag="RD477_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7634394.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="RD477_002040"
     CDS             1259..4048
                     /locus_tag="RD477_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDT7634395.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="RD477_002041"
     CDS             4095..4622
                     /locus_tag="RD477_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDT7634396.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="RD477_002042"
     CDS             4643..5833
                     /locus_tag="RD477_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7634397.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="RD477_002043"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="RD477_002043"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDT7634398.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8337..9011
                     /locus_tag="RD477_002044"
     CDS             8337..9011
                     /locus_tag="RD477_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7634399.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECVNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9008..9349
                     /locus_tag="RD477_002045"
     CDS             9008..9349
                     /locus_tag="RD477_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634400.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLI
                     AVTKEKQIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTKEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9379..9738
                     /locus_tag="RD477_002046"
     CDS             9379..9738
                     /locus_tag="RD477_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDT7634401.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10262..12343
                     /gene="cadA"
                     /locus_tag="RD477_002047"
     CDS             10262..12343
                     /gene="cadA"
                     /locus_tag="RD477_002047"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDT7634402.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12654..14120
                     /locus_tag="RD477_002048"
     CDS             12654..14120
                     /locus_tag="RD477_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7634403.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14614..15153
                     /locus_tag="RD477_002049"
     CDS             14614..15153
                     /locus_tag="RD477_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HDT7634404.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITGFPLLPLDLLWHTTDYSIQA
                     KKWFIQQQQSFMETNHSWIIEGNYTSTLGKRIKEADKIIWLQVPRCRAIYRVILRSLK
                     RKINKKTRPDMPETFSERFDKEYLEFLSFIWHFEETHEPEIQQLIQENHAQNKLIILK
                     TRGEKQEFLTKLKIESKNN"
     gene            15227..15667
                     /locus_tag="RD477_002050"
     CDS             15227..15667
                     /locus_tag="RD477_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDT7634405.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15680..15889
                     /gene="copZ"
                     /locus_tag="RD477_002051"
     CDS             15680..15889
                     /gene="copZ"
                     /locus_tag="RD477_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDT7634406.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15889..18075
                     /locus_tag="RD477_002052"
     CDS             15889..18075
                     /locus_tag="RD477_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDT7634407.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDALFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18095..20266
                     /gene="copB"
                     /locus_tag="RD477_002053"
     CDS             18095..20266
                     /gene="copB"
                     /locus_tag="RD477_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDT7634408.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(20330..21918)
                     /locus_tag="RD477_002054"
                     /pseudo
     CDS             complement(20330..21918)
                     /locus_tag="RD477_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         7228 a         3836 c         4731 g         6123 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cctatttgac caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagaggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggagatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggata tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 atgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gccaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgagcag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggccgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccaa tcaactagca atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 cactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gccggaagaa gttttccaaa cagctaaaaa
     6061 tctgcaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gccgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctacg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat cgatccgccg atcttgactg gttccgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgattgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagct atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacagat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 ccttgaacga acagtcactt ggattgctgg tatcgaccat gtgcgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagtgcctt
     7441 gacctgagta ttagagaaac aaaacggaaa aatagcttct catattttac cgttttttta
     7501 gctaatatcg agggtcagct gattgaacac cgtttatatc aagaggttgt gcgcctgccg
     7561 ttcagctccg agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt
     7621 gtgacttgtg gccggagagc aacgtttatt taaaaaaaag aactgcgaac tctcgcagtt
     7681 ctttttttaa aatacgggat aatggaagtt tccagcagac aagagaagaa agcaaaagaa
     7741 attgctattt gattcacaag gttctataac gaaatcgcaa tcaatcaaac atttacaaag
     7801 aaggtaattc aaatggaaat ctgatttgat gatgcttctg ctgttgaaat aaagcagcat
     7861 ctagcacctg aaaacaaact aatagtaaca ttcgaagatg gagtaggtcc gtattttcag
     7921 catgcaataa tccatatgta agttcaattc acccttgaaa tcatcggacc agataaagtt
     7981 atgaccaatc tatcccaagc aatctagaag agatattagt aaagggacat ttactagaag
     8041 tcttagatga gcctataaat atatgttttg atggcgtcat cagtggtctg attgacggaa
     8101 gcttgggtat gatcgatctc tctggaccaa acaacgaagg aatcaagggg aatccggcac
     8161 gttaagaaaa aaatagcata aaaaactaga cacttgatga gcgttcattc aattgtttga
     8221 agggactagg ctggtgtcat tttttataca tatattttct ctttcatctt gatagggctt
     8281 atgggataat ataaggaaga aaatcatcgt tttattcaaa aaggagtttt tgacaaatga
     8341 aaaaacgtat attatttatc ggtgatagca ttacagacgc aggaagagac cgagcagaag
     8401 caactgatct aggtaaaggt tatccacttt tgattgccgg aaaactaatc tcccaatatc
     8461 caacgatgga atttgaatgc gtgaaccgtg ggataggagg agacaaaatt gcagatctgc
     8521 aagtacgctg gcaagaagat tgtttagctt tacaacctga ctatgtatcg attttgatcg
     8581 gaatcaatga tacctggcat aatactagcg atcaggctat attcggaact gagaaatctg
     8641 ctcagcaatt tgaatccgtt tatcgaaaat tactaaatga acttcaaaaa gcgggaatct
     8701 gcaacatcat cttgatggag ccgtttgtac ttcctataac cgacgaccgt catggctggc
     8761 gtaaagactt agaccctaaa atccaaattg ttcgtaaact tgctaaggaa tatggcgcat
     8821 tgctggttcc tttagatggt atattcaatg ctttagggat tgcttatggt ttttcgaaat
     8881 atacaagaga agatggggtc cacccaacga ttggcggaca tgagatcatt gcacaaagct
     8941 ggatagatgc aattgccgca gtcgattttt ttagtggaga taaaaatgag cgaaagaaaa
     9001 gtgaaaaatg atggaaataa ggtgggcagt aaaagaagat ttacctcaaa tcaatcagct
     9061 gattactgat catccagaat cattcgtaaa agcaaaaacg gcagctgacc taaatgataa
     9121 gaataaacta ttgctcattg ctgtaacaaa agaaaaacag attatcggat ttgcgcaagc
     9181 ggacgtgcaa cagtcagaag aacgattgga tcagcgacca tcaggaaaac taatccaaca
     9241 cacattagta acaaaagaaa atacgaaaga aatccaaaaa aaatttgcag aatacttgat
     9301 gctggaagct aaaaaaagaa gaataaaatc aatttttgcc cagttttaat caaaaacagt
     9361 aatagaagga gaaatacaat gagttgttta ggaaatgtca tctggtttat attcggcgga
     9421 ctaatcggtg gtcttagctg gttagcagcg gggatacttt ggtgtatcac gatcataggt
     9481 atcccaatcg gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc ttttggcaaa
     9541 gaagtaattt atcaccagtc tggcatgtca ttgatcgtta atatcatctg gctgatcgtt
     9601 tcaggcctac caattgctct cgcacatgtc gtcagcggca tccttctatg tatcacgatc
     9661 atagggatac cttttgccaa gcaatctttc aaacttgctc gattggcgtt gatgccattc
     9721 ggtgcagaaa ttgtataaaa gagaataata aaggttagat aacgcataga gtcaggtggg
     9781 atacatttat ttcacctgac tctatgcgtt ttcctgtata agttcttcca tgtgactttt
     9841 acgtctttta tttatatggg attactgttt atggaaagtt gaaataattt tcattaaaga
     9901 actctttcta tatttttgaa agcgtagtgg agcttcgaat atctgggaag gacattttgc
     9961 agggattgaa aaaagatatt gtcctatcga aaaattaatg aatttagaca aattgccagc
    10021 aacaggcttc aaacttagtt gctatcctgt caagatcaag aaggcaagtg cgggttggat
    10081 aagagcagtg gcaatgatcg aaaagaaaaa gaaagaataa gccaaaacat ctgtatggaa
    10141 gaaattttcc gatgcagatg tttttcattt tttctttcgg ataccttgac atatgaataa
    10201 ccattcattt ataattagtt atgaagagat attcatatgt tgaggcgagg aggtcattaa
    10261 aatgaaaagt tacagattag aaggtttaga ttgtgcgaac tgtgcaatga aaatcgaaaa
    10321 aggagttcag aaaatcaatg gagtcaagga agcaacggta aacttcactt ctggaaaact
    10381 aacgattgac gcagaggaag atcatttggc tacgattgaa caagaaacaa aaaaagtagt
    10441 gaaggaatta gaaccagatg tcaaagtaac agaaatagat aaggaaaaag tatctgaaca
    10501 tggaaatgaa aaagagagaa atacgctgtt ccggatttta ttttcccttg caggtatcgc
    10561 tttattgcta ttatttgatt ttaatgaacc aattcgcttg atagggtatt tattgattta
    10621 tctgctgata ggatatgatg tagtgaagaa ggcagtgatg aatatcgtca aaggaaaaat
    10681 ctttgatgag aattttctta tgtctgttgc tacgattggc gctatgttga tcggtgaata
    10741 tccagaagcc gtagcagtga tgctatttta tcaaatcggc gagtatttcc aaggattggc
    10801 agtcagtcat tcaagaaaat cgattcgcga attgatggcg atccgtccag aaacagctca
    10861 cgtgcagacg gcggaaggat tgatgaccgt gaaccctgaa gacgtgcaga tcggtcagtt
    10921 tgtacttgtg aagcctggtg aacgtgtacc gttagatgga acgatcatcg aaggagaatc
    10981 attagtcgat acgtcagcac ttacaggaga atcagtccct aaatctgtat atgttggtga
    11041 aacagtatta agcggattta tcaataaaaa taaaccattt ttagttcaag tagaaaaatc
    11101 ttatgaaaat tctactatca gcaaattatt agaattagta gaaaatgcca gcagtaaaaa
    11161 agcaccagct gaaaatttca ttactaaatt cgcgcgctat tacacaccag tcgttgtagg
    11221 attggccgtt ttattagctg ttttacctcc acttgttgtt tctggagcgg cgttttctga
    11281 atggatctat cgagcattga catttttagt gatctcatgt ccatgtgcat tagtgatttc
    11341 cgtgccactt agtttctttg gcggtatcgg aggagcaagt aaaatcggtg tattagtaaa
    11401 aggttcaaat tacttggagt tgcttgcaca aactgagaca gtggtattcg ataaaaccgg
    11461 cacattaacg aaaggtgact tttcgatcca gacgatcgat acaaaaatcg atccaaagat
    11521 attcatgcaa tatgttgcga gtgcagaaca gttttctaca catccgattg ctcaatctgt
    11581 tttagaaggc tacgcaggtc cgcttttacc aacaactaat atccaagaat ttgcaggtga
    11641 agggatctta gctgaagttg atggcaaaca agtattagta ggtaatcaca aattgatgga
    11701 aagatttgaa atctcttttc cttcaagtca agaaatcggt acattgcttt atctagcgat
    11761 cgatcaaagt tacagtggtt atcttgttat tgcagatacc ttgaaagaag atgccgtaga
    11821 cgcattggtt caattaaaac aggctggtgt gaagaacaca gtcatgttga cgggcgactc
    11881 gaaaaaaatt gctgatcata tcgggaagca agtaggcgtg gacaagatat atagcgaatt
    11941 gcttccagaa gataaagtgc agcggttaga ggaaatctta caaagaaaca ataaaaagac
    12001 cgcatttgtc ggagatggaa tcaacgatgc accggtgcta gcaagagcag atgtaggaat
    12061 cgcaatgggt ggattgggaa gcgatgctgc aattgaagca gcagatgtag tcatcatgaa
    12121 tgatcaacca tcgaagattg cagaagcaat ccatttagca aagaaaacat tgaaaatcgt
    12181 caaacagaac attgtctttg ctattgggat aaaaattttg gtacttgcct taggtgcttt
    12241 tggttttgca tcaatgggag atgcagtatt tgcagatgtt ggggtgacag tattagctgt
    12301 tttgaatgcc atgcgaagct tgcatgtaaa aaataaacgt tgatcaatga agaggacgaa
    12361 tgaacgtcct ctttttttta tatccttttc ttaatgaaaa atgtaaagaa aatagataag
    12421 attataaaat aactaatgtt tttaattttg cagaaaattc aaaatagctt gaaattcaat
    12481 aaggtcaatg aaataaaaga aaataactta tttttttgat ggaaaaaata tgaaaaaacg
    12541 atgtgtatta aataaaagaa tacacacata aaataacact gtcttgtaag tttgttaaaa
    12601 attgaatatg attacatctg gataacaaat ttttatcata aaggagagaa cgaatgaaca
    12661 acaaacaatt acgctggttt actgttgcgt taattgcatt taacatggtt tggggcatgg
    12721 ggaatgtagt taataactat gctcagcaag gagtatcagt tgtcacctct tggctattga
    12781 ttttggccat ttattttatt ccatacgcat tgatcgttgg acaattagga tcagccttca
    12841 aggatagtaa aggaggcgtc agttcatggg tcgaaaatac gacatctaat aaacgactgg
    12901 catactatgc cgcttggact tactgggtcg tccatattcc ttatttggca caaaagcctc
    12961 aagcaatcct gattgctggc ggttgggcag tccaaggaaa tggaaatatc gtaaatacga
    13021 tgtcagtaca aatggtagca gttatttcac ttattatatt tcttgccttt ttatatttat
    13081 caacaaaagg actttctact ttgaaagtga ttggtggact ggccggaaca gcgatgttcg
    13141 tcatgtcgat cttatttatt cttttagctg taacggcacc gttcatcaat ccaacgatgg
    13201 aatttgccac accacatatg aatcaagtga aaacatatat tccaacattt gatttcagtt
    13261 atttcacaac cgtttcgatg ctagtatttg cagttggagg agcggaaaaa atatcccctt
    13321 atgtcaactc tacaagaaat cctgcaaagg aatttcctaa agggatgatc ttcttagcag
    13381 caatggtagg gatttgtgct gtattaggct cagtcgcaat gggtatgctg tttgctagcg
    13441 ataatattcc taaagacttg atggcaaatg gggcatacag tgcttttcaa attttaggta
    13501 attactatgg tatcggtaac tcattgatga tcatttacgc attgacaaac atgattggac
    13561 aaatctccgc attagccttt tcaattgatg caccactaaa aatcttgtta gcagatgcgg
    13621 atccagaatt tgtaccaaac tggctacgta aacgtaatcg taaaggtaca ttggtgaatg
    13681 gttatcttct gacaggtata ctagtaggaa tcattatttt gttgccgatc tttggtatca
    13741 aagatatgaa tgaattagta aaatggttga cgaacttgaa ctcagttgtc atgccaatgc
    13801 gttacctatg ggtgttcttg gcatatatga tgttgaataa agcttataag aaattcaatt
    13861 ctgaatataa atttgttaaa aatccaaaaa tcggttttat ctttggtgct tggtgctttg
    13921 ccttcactgc gtttgcatgt atcctaggaa tggtgccaaa agtagattat actgctgatc
    13981 ctaaagcatg gtggttccaa ttattcacga atatcatcac accaatcgtc ttgatcttat
    14041 taggtatgat cttgccagca attgctcgtc gagacaaaaa caaagaaatc actgaaccag
    14101 aaacagtggt tcagccttaa aatagaaata cttgttacaa gaggttgtga cagaaggggt
    14161 ctcagcttca agaaataaga aggaatgtac gaaaattgct tctcaaattt ttgtgaagtt
    14221 tcggcttgtt tttcgaagaa gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag
    14281 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcccctcata tttttcgttt
    14341 ggctgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtaaa
    14401 atcagcgatt tgacctgagt attagagaaa caaacggaaa aatagctcct catattttgc
    14461 cgctttttga gctaatgctg aaggtcagct gattgaacac catttattcg aaacaaggga
    14521 ctttgacacg acttttgtca cagtcccttg tttttttgcg gcgttataag tggtatgatc
    14581 aaatcaaaat gataagagaa gtgagggaga agaatgaaga tcatcgtgat tggacaaagt
    14641 gggtcgggaa aatcgaccct cgcaaggaag atcaaagaaa tcaccggctt tccgctactg
    14701 ccgctagacc ttttatggca tacgacagat tacagcatcc aagcaaaaaa gtggttcata
    14761 caacaacagc aatcatttat ggagactaat cattcatgga tcattgaagg aaactatact
    14821 tctacactag gaaagaggat taaagaagca gataagatca tttggctaca agttccgcgt
    14881 tgccgggcga tttatcgagt gattttacgt tccctcaaaa gaaagataaa caagaagacc
    14941 cgaccagata tgcctgagac attttcagaa agattcgata aggaatatct ggaatttctg
    15001 tctttcatct ggcattttga agaaacccat gaacccgaaa tacagcagct gatccaagaa
    15061 aaccatgcac aaaacaaact gattatcctt aaaacaagag gggaaaaaca agaattttta
    15121 actaaattga aaatagagag caaaaataat tgacagtttt tgtttacaag tgtaatctgt
    15181 atccataagt tattgattac atttgtaaac gaaaggtgtg aaataaatgg aaaaagtcaa
    15241 aacgccagta aagatcagtg attcagagtg ggagatcatg cgtgttattt ggacacttgg
    15301 acagacgaca gcccaagaaa tcacgcagat cttagctgat tcgatggatt ggaaaccagc
    15361 aacgatcaaa actttgctgg gacgtttggt aaaaaaagaa gtgatctgga cagaacaaga
    15421 aggaaaaaaa ttcatttatc atccagctgt ctcggagatg gaaacagttc gcagtgcgac
    15481 ggaaaatctt ttttcccata tctgtgcaaa aaggattgga gagacgattg cggatctagt
    15541 agaagaagcc actttgactc aagaagatat caatgagatt ttgaaacaac tggaacaaaa
    15601 gcaaccagta gatacgatcg aatgtaactg tatcccaggt caatgtgaat gtaaacaaca
    15661 tcaataggag gaagaaaaaa tgaaacaaca attttcaatc gaaggtatga gctgcaatca
    15721 ttgtgcagca agagtagaag aaacagtaag cgcacttgac ggcgtgcaaa aagtgaaagt
    15781 aaatttgaaa aaagcaaacg gtacggtcaa gtttgatgaa acaaaagttc aatccgaaaa
    15841 gatttgtcaa gcaatcaatg gattgggtta taaagcagag gtgatttgat ggcaacagca
    15901 gaaaccaaaa tggatacgtt cgtcattaca ggtatgactt gtgcaaattg ttctgctaga
    15961 atcgaaaaag agctgaacgg acaacctggt gtggtcaatg ccacagtcaa cttggccact
    16021 gaaaaagcaa gtgtcaaata tgaagggacg accactgaaa aattaatcca aagcgtagaa
    16081 aacatcggct atggtgcaat tttgtacgat gaagcccaca aacaaaaaat tgcagaagaa
    16141 aagcaagcgt atttgaaaaa aatgctattc gatcttatat tgagtacggt tttgactttg
    16201 ccgttgatgt tgtcaatgat tgctatgatg ttaggaagcc atgcggcgat tgttcacttt
    16261 tttcattttc caatcgtcca gttagtgctg tctgctcctg tccaatttta tgtcggtgct
    16321 cgtttttata aaggtgcgta ccatgcaatc aagacgaaag caccaaatat ggatgtattg
    16381 gtagcaattg gaacatcggc tgcctttgca ttgagcattt acaacggatt tttccgtgga
    16441 cacccccaag atctttattt tgaaagcagc agtatgatca tcacgctgat tttgctcggc
    16501 aaatatttgg aacatacagc aaaaacgaaa acaggtaatg caatcaaaca attgatgtct
    16561 cttcaaacaa aaaccgccca agtgataaga aacgggaaag aagaaacgtt agcgattgaa
    16621 gaagtagtcg ttggtgatca actagtcatc cgtcctggtg agcagattcc agcagatggt
    16681 cgaattattt caggaagcag tgcgatagat gaaagtatgc ttactggtga aaatcttcct
    16741 gtagaaaaga atccggatga cgcgcttttt ggcgggacaa tcaatacgaa tggtctgcta
    16801 cacatggaag tcacacaagt cggcaagcag acagtattag cgcaaatcat tcaaatggtg
    16861 gaagatgctc aagggagcaa agcaccaatc caaaaaatcg cagatcggat ctcagggatt
    16921 tttgttccga tcgtattagt catcgcgttt atcacgctca tagctacagg tttgatcact
    16981 ggtgattggc aactcgcatt gatccatagt gtctctgtat tagtcattgc gtgtccatgt
    17041 gcattaggtt tagcgactcc aacagctatt atggtaggaa cgggtgttgg tgcacgaaat
    17101 ggaattttaa ttaaaggcgg agaagcatta gaagcagctg ctcacttaga cagcattgtt
    17161 ttggataaaa ctggaacgat tacagaagga aagcccaaag ttacagatct agtcggttct
    17221 aaagaagtcc tatctatttt ttatacttta gaacaagctt ctgaacatcc actaggtaaa
    17281 gcaatcgtgg aatacggaaa actgcaagaa gcagcaacat acgatatgat tgattttaca
    17341 gcccacccag gtgctgggat cagcggtacg attaatggag tacgctattt tgcgggtaca
    17401 cgtaaacgat taattgaatt gaacctatca tttgatgaat accaagaaca cgcgttagaa
    17461 ttagaacagc agggaaaaac agtcatgttt ttagctgatg aaaagcaagt gatcgggttg
    17521 attgccgtgg ctgatcaaat caaactagaa gccaaacaag caatcaagca gctccagaat
    17581 aaaggattag atgtttttat gctgacagga gacaacaaac tggcagccga aacgattggt
    17641 aaacaagttg gtattgatcc aaagcatatc tttgctgaag tacttccaga ggataaagca
    17701 gcctacgtag aaaaacttca gaaagatggc aaaaaagtcg gtatggcagg cgatggaatc
    17761 aatgatgcac cggccttagc attagctgat gtcggtatgg caatgggaag tggaacggac
    17821 attgcgatgg aaacagccga tgtgacgctg atgaatagca gtttggcatc cattgcacaa
    17881 acaatcgagt tgtctaggtt gacattgaga aaaatcaaac aaaatctttt ctgggctttt
    17941 gtctataata cgattggtat tccttttgcc gcactaggct ttttgaatcc aatcattgca
    18001 ggaggagcga tggcgtttag ttccgtcagt gtattgttga actccctgag cttaaatcga
    18061 cacatgaaaa agtaagagaa gggatggagt aaaaatgaat gggaacgata tggagaaaaa
    18121 acatgagcat aaaaaagaag tagtaaacaa agaaagtaca gaatctcatg aaaaacagat
    18181 ggagatgaaa catgaccata gtcagatgga tcattccatg cacatgggac ataaccatgg
    18241 agatatagca aacagcaaac aaatggacca ttcgatgcac atggatcacg aacacggcgg
    18301 catggatcat tccatgcaca tggggaattt caaacaaaaa ttttggctat cgcttatcct
    18361 agctatccca atcattcttt tttcaccaat gatgggaatg gaatttccat ttcaagtaac
    18421 gtttcctggt tctgattggc tcgtattgat cttagcaact atactattta tctatggcgg
    18481 tcagccattt ttaagtggag ctaagatgga attgaaacaa aaaagtcctg ctatgatgac
    18541 actgatcgcg atggggatta cagttgcata tatttatagt gtctattcgt ttatcgcaaa
    18601 tctgctcaat ccacatacac acgtcatgga tttcttctgg gaattggcta ctttgatcgt
    18661 tattatgcta ctaggtcact ggattgaaat gaatgctgtg tcgaatgcca gcaatgcttt
    18721 acaaaaatta gcagaattac tgccagaatc agtgaaacgc ttgacgaaag atggaaaaga
    18781 agagacagtt tcattgaaag aagtaaacga aggtgatcgt ttgatcgtcc ggtctggtga
    18841 taaaatgcca acagatggtg taatcttgaa aggtgaaaca attgttgacg aatcagctgt
    18901 gactggcgaa tcaagaggaa tcaaaaaaca agccaatgac aaagtgattg gcggttctat
    18961 caatggggat ggaacgatcg aaatcgaagt aactggaact ggtgaaaatg gttatctagc
    19021 gaaggttatg gaaatggtgc gaaaagcaca aggagaaaaa tccaagctag agtcattatc
    19081 tgacaaagtg gcaaaatggc tgttttatgt cgcattaatt gtgggtatcc tggcattcat
    19141 cgcctggttg ttcttgacag atcttcctaa tgcattagaa cgtatggtaa cagtctttat
    19201 catcgcctgt cctcatgctc taggtttagc tatcccatta gttgttgctc gctccacatc
    19261 aatagcagct aaaaacggat tacttttgaa aaaccgcaat gcactggagc aagccaatga
    19321 tctagatgtg atcatgctgg acaaaacagg aacactaaca gaaggaaaat ttactgtaac
    19381 gggtgtcgaa gtacttgatg atgcgttcaa taaaaatgaa atccttcaat atctaggagc
    19441 cttggaagcg aatgccaatc atccattggc tgttggcatt atgaattatt tgaaagaaca
    19501 tgaaatcaag ccttatcaag ctgaaaatct aaaaaatctt tcaggagtcg ggctagaggc
    19561 aacagtcaaa aaacaacaag tgaaaatagt taatgaaaaa gaagttgagc gtctcggatt
    19621 aaacgtagaa caagcattat tgaaacctta ccaagaacaa ggaaatacca tcagcttcct
    19681 gattttagaa aatcatctag ctgcaatcgt tgctttaggt gacgtagtaa aaacagaagc
    19741 aaaagaattt atccgaacat tgaaagaaag aaaaatcact cctgtcatgc ttactggtga
    19801 caataaaaac gctgcccaag cagtagcaga ttatttaggg atcgaagaat attatggcgg
    19861 tttattacca gatgataaag aagctgttgt ccagaaatac cttgaccaag ggaaaaaagt
    19921 aatcatggtg ggtgatggga tcaatgatgc gccaagttta gcccgtgctt caatcgggat
    19981 ggcaatcggt gcagggacag atatcgcaat cgattctgcg gatgtcgtat tgacaaacag
    20041 cgatccaaaa gatattttgc attttcttga tctagccaag caaacgcgaa agaaaatgat
    20101 ccagaacctc tggtgggggg caggttacaa tattcttgca attccgctag cagccggaat
    20161 tcttgcgcca attggtatca tattaagtcc agcagttggt gctgtcttga tgtcgttaag
    20221 tacggtagtt gtagctctaa atgctttgac attgaaaatc aaataataat gaaaaaaggt
    20281 tgtgacaaaa aacgtgtcac aacctttttt tcgagtaatc gatactcaat cagctggttc
    20341 tcggcattcg agaagtagat tctccagaaa ttttatcttg ttttttgaag cggctccctt
    20401 ttgtcagaat ttttttgtat accgtttttc ttattatatg cgttatttct aaataggttt
    20461 ttttatcgaa ttttaccgga tctgcttgtc gtccatatat ttctgcttca tattgagtag
    20521 ttaatcgaat gaaacgcaga tctagcttga attcttgttc tattcgcagg ctatattcct
    20581 gcaaagattc tgcgggtttg cgcggtacca atgtttccaa agggttaaga agtttttgat
    20641 atgcaatcgt attagtagaa gcaaaaaaat gaagatagat aagataactt gtccagcgca
    20701 gagattttct gaataacaat agaaagaaca acaagacgga taaacacact ccaaggctga
    20761 ataagacgat tttataagct ggtgtgagtg ttgtttcttc tgatggaatg acctcagatc
    20821 tctcactgga aatagtttct gccatatttt cgctttcatt tggttgttcg atagcaggct
    20881 ggacggtttc ggttgtttca gcaactggtt gtgaacccat tggattggca aatgaagggg
    20941 ttggttcaaa tggaagccag ccaaatccag gaaagtatac ctctggccaa gaatgagcat
    21001 gactattgag aatgctgtat tcctcattcc cttgattatc tgtgcctgtt ctgagcccat
    21061 ttgtaaaacc ttttgtccag cgagctggga taccaagtgt tcgaagaagg acgaccatag
    21121 aagttgaaaa gttgtcacaa tagccgactt ttgaatcaaa gaggaaataa tccacataat
    21181 cgtgttcttt cggagtatgc ccggtatccg ttttggaata gcggaaattt ccgcttgttt
    21241 ttaaatactg ttagacagct tcgactttac gaattaaagt tgcttcattt ttggtcaatt
    21301 cgaccgccaa gtcctcaata cgtttaggca aactttttgg caattgaaga tttgtctgct
    21361 gattttctaa atcatcagct tttaactgac tgctttttaa ttcttctctc gtaaaaacta
    21421 acctttctgt ttgtaaagtt gcagaacgtg tcgattcatt cgggacagga atgaatcgct
    21481 ggctttcagg caaatagtaa agcgtttgtc cactagctaa tgaaaaatca gtcaaggtag
    21541 tatttccata aggataaggt aagaaagctt gtttttttag ggtcaatgta atgttttctt
    21601 tgtcttctat aacggtttgg tagctcgtga tgtcaacagg gaattcttgg attggttgga
    21661 tgggtgcgtc ctgatcttct atccatccag aaccggtgta atgattttta ttttcgatct
    21721 tccaatagcc atttcctttt tggttcgctg taaaaacaac ttcgccatta tcatataaag
    21781 gtccaccaag ctgttcatca ttctcactga agcctgttct tgcacttccg gttaattgat
    21841 actcatggag tgtgtcataa agcccttcat cattaagata ttctcggatc ggttcactcg
    21901 tttcctcgag agattggt
//
LOCUS       DAPBBG010000048        21869 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPBBG010000048 DAPBBG010000000
VERSION     DAPBBG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.048"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(266..466)
                     /locus_tag="RD477_002055"
     CDS             complement(266..466)
                     /locus_tag="RD477_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7634409.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="RD477_002056"
     CDS             complement(771..2003)
                     /locus_tag="RD477_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDT7634410.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2257..2826
                     /locus_tag="RD477_002057"
     CDS             2257..2826
                     /locus_tag="RD477_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7634411.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3004..3444)
                     /locus_tag="RD477_002058"
     CDS             complement(3004..3444)
                     /locus_tag="RD477_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDT7634412.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3602..4366
                     /gene="map"
                     /locus_tag="RD477_002059"
     CDS             3602..4366
                     /gene="map"
                     /locus_tag="RD477_002059"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDT7634413.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4398..5321
                     /locus_tag="RD477_002060"
     CDS             4398..5321
                     /locus_tag="RD477_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDT7634414.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5397..6536
                     /locus_tag="RD477_002061"
     CDS             5397..6536
                     /locus_tag="RD477_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDT7634415.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQLILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6529..7329
                     /locus_tag="RD477_002062"
     CDS             6529..7329
                     /locus_tag="RD477_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7634416.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7329..8156
                     /locus_tag="RD477_002063"
     CDS             7329..8156
                     /locus_tag="RD477_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7634417.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8134..8868
                     /locus_tag="RD477_002064"
     CDS             8134..8868
                     /locus_tag="RD477_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7634418.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8968..9834
                     /gene="rfbA"
                     /locus_tag="RD477_002065"
     CDS             8968..9834
                     /gene="rfbA"
                     /locus_tag="RD477_002065"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDT7634419.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9848..10420
                     /gene="rfbC"
                     /locus_tag="RD477_002066"
     CDS             9848..10420
                     /gene="rfbC"
                     /locus_tag="RD477_002066"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDT7634420.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10442..11470
                     /gene="rfbB"
                     /locus_tag="RD477_002067"
     CDS             10442..11470
                     /gene="rfbB"
                     /locus_tag="RD477_002067"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDT7634421.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11568..12419
                     /gene="rfbD"
                     /locus_tag="RD477_002068"
     CDS             11568..12419
                     /gene="rfbD"
                     /locus_tag="RD477_002068"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDT7634422.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12454..14487
                     /locus_tag="RD477_002069"
     CDS             12454..14487
                     /locus_tag="RD477_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634423.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14531..15811
                     /locus_tag="RD477_002070"
     CDS             14531..15811
                     /locus_tag="RD477_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDT7634424.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16021..16827
                     /locus_tag="RD477_002071"
     CDS             16021..16827
                     /locus_tag="RD477_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634425.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16839..18059
                     /locus_tag="RD477_002072"
     CDS             16839..18059
                     /locus_tag="RD477_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634426.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18049..19635
                     /locus_tag="RD477_002073"
     CDS             18049..19635
                     /locus_tag="RD477_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7634427.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19674..21812
                     /locus_tag="RD477_002074"
     CDS             19674..21812
                     /locus_tag="RD477_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7634428.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6897 a         3706 c         4545 g         6721 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt
     2221 ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact
     2281 agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt
     2341 actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt
     2401 aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat
     2461 gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc
     2521 cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga
     2581 ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca
     2641 gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata
     2701 gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt
     2761 gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc
     2821 gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag
     2881 gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa
     2941 tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt
     3001 atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc
     3061 ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa
     3121 agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc
     3181 gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac
     3241 gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc
     3301 ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag
     3361 attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc
     3421 atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat
     3481 gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca
     3541 tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa
     3601 tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt
     3661 agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat
     3721 tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt
     3781 tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc
     3841 tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa
     3901 aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga
     3961 ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg
     4021 gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc
     4081 gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt
     4141 tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat
     4201 cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc
     4261 gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga
     4321 tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat
     4381 aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc
     4441 gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg
     4501 tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt
     4561 taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg
     4621 agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa agttcaggag
     4681 gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt
     4741 tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc
     4801 gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg
     4861 ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga
     4921 actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca
     4981 tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc
     5041 cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg
     5101 ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca
     5161 tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg
     5221 ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt
     5281 tcaataaatt gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt
     5341 aaatttatat cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc
     5401 ttgtttcttt tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa
     5461 cacctgtatt caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa
     5521 gaataaataa agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta
     5581 tatcgacttt attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga
     5641 ttgcttcttt agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta
     5701 aacaaaaaag tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac
     5761 gtattgattc cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc
     5821 ccgtaacgat tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg
     5881 atgggctggc ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt
     5941 ttttcctcca tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta
     6001 ttatcggttt ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag
     6061 ctctattcct agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt
     6121 ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg
     6181 ctatcattag gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc
     6241 atcaccgact aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga
     6301 tggctttgat gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga
     6361 tcatattgat tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga
     6421 tcaatgagaa acatcagtta attttaaaaa cattgcgatt tttagggaat aaagagtacc
     6481 gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa
     6541 ttaaaccagc cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca
     6601 ctagataatt ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac
     6661 aacaattcac ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc
     6721 gatatctatt ttcatgatga aaattccgga tttggttatg gacataatca caatctgata
     6781 gctagcgatt ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca
     6841 gttacagcat tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag
     6901 attcttaatg aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat
     6961 tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct
     7021 gtgtttgaat gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt
     7081 agttttatgg tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc
     7141 ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta
     7201 ttgtacacac ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca
     7261 aaactgtttc gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt
     7321 tttttctgat gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc
     7381 aatctccagc ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta
     7441 tttacgataa tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt
     7501 atcatcatga tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag
     7561 cagatgaagg aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt
     7621 attttgaact attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga
     7681 ttttttctgg gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag
     7741 ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg
     7801 ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag
     7861 attttttgga tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc
     7921 ttcctgtacg aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc
     7981 gataccgttc tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt
     8041 tatccagtca ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa
     8101 accggcatat ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc
     8161 gtgcgttcgg tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat
     8221 agtattttat ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta
     8281 gatgatacat gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt
     8341 tcggggcctc gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga
     8401 gatctaatct ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc
     8461 actgactatt ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg
     8521 gacagcgagc tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga
     8581 tggcttaata atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct
     8641 ttaaaacccg atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt
     8701 ttgctgggag gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat
     8761 gatttaaacg cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt
     8821 gttcatctga ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt
     8881 aaatccttaa tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt
     8941 tgaaatcaaa aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga
     9001 acacgtctgt accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa
     9061 ccaatgattt attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt
     9121 atttcaacgc cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta
     9181 ggactgcaga tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc
     9241 attggagaag aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac
     9301 tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca
     9361 gtatttggct atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat
     9421 atgcaagctt tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact
     9481 ggcctttatt tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa
     9541 cgtggagaac tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc
     9601 gtagaactta tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt
     9661 gaagcatcta cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg
     9721 gaagaaattg cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag
     9781 ccattgaaaa aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc
     9841 cttacagatg aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt
     9901 ctttggcgat catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca
     9961 tggattgaaa tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct
    10021 tcgggggctt catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac
    10081 tggagcagtt ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg
    10141 ggacggattt atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc
    10201 ccatggattc gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta
    10261 taatgcagaa gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc
    10321 aattgatcca tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga
    10381 atttgaagca gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct
    10441 tatgaaaaac atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta
    10501 cgtggtaaac aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg
    10561 aaacaaagag aacttggccg gacttcctag cgatcgcgta gaattggtcg taggagatgt
    10621 tgcagatgca gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc
    10681 ggcagaatcc cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat
    10741 cattggtacg tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt
    10801 atctactgac gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga
    10861 aggcgaagga gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc
    10921 tacaaaagct ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc
    10981 aaccatttca aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc
    11041 tcgccaagta acgagtatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa
    11101 tgttcgtgac tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa
    11161 aggcagaatt ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt
    11221 gatggaattg atcttagaaa tgatgggcca accaaaagac gcatacgacc atgtaaacga
    11281 ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca aaattgcgag aagagctagg
    11341 ttggcagcct gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga
    11401 agaacatgaa gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa
    11461 tggacaataa tacagaggaa atttcactgt attaatggaa aaacacatgt gaggcaggcg
    11521 ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga taaccatatg tttttattaa
    11581 ctggaggaaa tggacaatta ggtacagaat tgcgccattt attagatgaa aaaaatctgc
    11641 catatgtttc aacagatgca aaagacttgg atatcacaga tgctgagaaa acaatggctt
    11701 atattacaga attaaaaccc gatgttattt tccattgcgc agcttacaca gcagtagata
    11761 aagcagaaga agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc acaagaaatg
    11821 tagcacttgc agcaaaagct gtgaatgcac aattcgtcta tatcagtaca gactatgttt
    11881 tcgatggaaa aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg
    11941 aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt
    12001 atatcatacg aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc
    12061 aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa
    12121 cttggacacg tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg
    12181 gtatttatca tttgtcgaac gaaaatagct gttcttggta tgagttcgca aaagaaatat
    12241 tgaaagatac agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa
    12301 cacgtccgca gtattcagtc atgagcctga aaaagacaga agcgttaggt tttgtcatac
    12361 caacttggca agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat
    12421 ttccttttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga
    12481 cgaaaagaaa agccaaaagc gaatcaggca gattcgaatt tggtgcttga cctgtttcta
    12541 aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac
    12601 ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact
    12661 gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct
    12721 ttttacctct cacaagctga agaggacttt tcgttggtga acgaggatta ttcattaaac
    12781 ggacaaaaca tgatcattgc gtataattct cctgtaaagg atattacggt tatcggaaaa
    12841 ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg
    12901 atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagagg
    12961 aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg
    13021 tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat gattgctttt
    13081 tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg
    13141 gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg
    13201 tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg
    13261 gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt
    13321 catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga
    13381 aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat
    13441 tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat
    13501 cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc
    13561 gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt
    13621 accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta
    13681 agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat
    13741 atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca
    13801 tggtcttatt tctatgcgat tacgcattcg aaaatggata attatttttc ggattttgaa
    13861 accatcgcag ttgctgtgtt gggaacagtg attttagctt ttgcattatt tggctggaga
    13921 cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca
    13981 gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc
    14041 gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca
    14101 acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag
    14161 aaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct
    14221 tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg
    14281 ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa acgaccatta
    14341 actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac
    14401 ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact
    14461 caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt
    14521 agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt
    14581 cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag gggtaggttt
    14641 tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat
    14701 tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc
    14761 gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg
    14821 agacttactg tttcctttga ttattttatt ttgtttagta attaaatgga tcgagtggac
    14881 aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata
    14941 tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc
    15001 gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt
    15061 attgatcagt ggatttgcca gttcttttga acgatggtat gttcgaatca tcggtgggat
    15121 agcagtcgtt gcagcctttt taggtatttg ggtctgccaa tctagaggtg cattagtcgc
    15181 gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc
    15241 gccatttatt gcctacacgg ttacgattct tttagctttg ccgatttctt atctggcggc
    15301 tgtctctgaa aaagtcaatt tgtttaccgg tcgagaagat atttggcata aattctatca
    15361 gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccagcg
    15421 aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata tctatggatt
    15481 gataggattt gggattgctg ctttgctgct tatccttttt attggtcgct taacgttgaa
    15541 agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca
    15601 gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt atatcctgtt
    15661 ggctatggcc gttcatcgat atgatgaacc agaagttacg gaggaattct atgttatgga
    15721 agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc
    15781 tcattcttcc aggatccaac gctatcattg ataggcacaa aatacgagag aggctgtgac
    15841 aaccgtcgca gccttttttg gtttattgca attgtctagc atcttctgta aaatagaaac
    15901 gttagagaaa atcttgtatc atagaaaatt aagaaagaaa taagaacgca gctcggtacc
    15961 aaaaatatgg taaaatggca cttgactgta attcactgaa aatcgcttgg aggaacacaa
    16021 atgtttaaag atttgtattt atttatgaag gacatttatc agaacagaaa attactgcta
    16081 cagttttcga tcaatgactt caaatcaaga tatgcaggat cgtttttagg aatcttatgg
    16141 gcgtttatca atccgctatt tactgtattg atttattggc tggtatttgg tgttggactg
    16201 aaagcacaaa tgatcgatgc aaaataccca tttatcgtat atttagttac cgggatgatt
    16261 ccctggttct tcttctcaga tgcatttgca tcaactacgg ttgtttttcg ggaatatact
    16321 tatttagtaa agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa aatcctatcc
    16381 aatctgtaca cccatttatt cttcattctg attggcatag ctgtcgcgct tataaatggt
    16441 atatatcctt cagcaatgtc tttacagctg atttattatt tattttgcat gtgtgctttc
    16501 ttgacaggat tgacttggct gacggcatcg atccaaccat ttttacctga tatcatgcaa
    16561 ttaattacga tcgtgctgca gttgatcatg tggacattac cgatattatg gagccctaat
    16621 cagtttgatt caggtcttgt aagcatattg aaattaaatc cgctgtacta tgttgttcaa
    16681 ggttatcgtg agtcattttt aagtgaagct tggttctggg agcatggaac atatactttg
    16741 tatttctggg ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg
    16801 aaaccacatt tttcagatgt gctgtaagga gaatagctat gacagaatat gcaattagac
    16861 taaaagatat cacaaaatct tataatatgt acgcaaaacc ttcggatcga tttcgtgaag
    16921 ccctcaatcc ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg
    16981 tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta
    17041 caatgttgaa aatcattacg ggagtactga caccaacatc cggcacgatg gaaatcgaag
    17101 gaaaaatctc agctttgcta gaactaggat caggattcaa tcctgagtat tctggctatg
    17161 aaaatatcta tttaaatgga atggttctag gattttcaag agaagaagtc gatgaaatgg
    17221 tcgatgatat cattgaattt gctgacatcg gcgaccatat ttatcaacct gtaaaaacct
    17281 attcaagtgg gatgttcgtt cgtctagctt ttgctgtagc gatcaatgtg aatcctgata
    17341 ttttgattgt ggatgaagca ttggcagtag gagatcttga atttcaacta aaatgtatgg
    17401 aaaaatttac agaaatcaag aactcgggaa agacgatttt attcgtttct catgatatta
    17461 gttcgattcg ccgtttttgt gatcgttcct attggttgaa aaatggaaaa gttgtcgaat
    17521 atggtgatac gatggaagta acgacgaatt acgaaaactt tttgaagaaa aaatcagtta
    17581 aaacagtaga tcgaaataaa aaccaagtag aacgcttcgc agctccagat atcgtagacg
    17641 tgacgaaagc cgaattgttg gataaagata aaaatccagt ggatatgctg gaacaaggag
    17701 aaaaactata cgttaaagta acctatgaag taaaagacga tacaatcaaa aatccagttt
    17761 taggaattgc tctgcgtaca gtcgacaatt tttatgtgtg cggactcaat actctactag
    17821 atggtatcaa gatcccttgg aaaaaaggtg agaatatctt ttatctggag tatgaaaagt
    17881 taggtttgtt agctggtgaa tattattttg acgttgcggt ctttgaagaa aatgcaacgg
    17941 ttcctttggt atacaagaca aaatacatga acttgtttgt cagcggttct tatatcggag
    18001 aaggcatcgt ggtcttagac cataaatggg aagaagggac tcatagcaat gaaatatgat
    18061 tttgaaatgg acctagatga acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca
    18121 ggaagtaaag ttttagagtt tggtcctgga aacggccgat taacgaaaca tttgatcgga
    18181 gcaaaacaat gtgaagtgag cattgtcgag ctagataagg aattgttcga ttttgtcagc
    18241 gagtttgccc aagatggatt ttatggagat atcgaaagtt ttgaatgggc aaactattat
    18301 gctggacaaa catttgatta cgttttgttt gccgatgttt tagaacattt ggtagatcct
    18361 ggaaaaacgt tgaagaaagt tagagaattt ttgaatgaag aaggagaaat tctaattaca
    18421 ttccctaacc ttgcgcataa ttctgtcatg attgatttgt tcaataatca attgccatgg
    18481 gcttcttatg gtttgctaga tgaaacccat aattcttttt atactcatga tggcttccaa
    18541 aaagtttttg aaaaagcagg attatacatc aatattgaag attatttgta cttagctgta
    18601 ggtgatacag aattaaaatc aacttatgaa gaattgccgg aagctgtacg ttatgatttc
    18661 aaaatgcgac catttggcga agtctaccaa tacttcttct cattgatgaa acatccagta
    18721 gcacaaagtt ccattgcgca gccgcaaaat tctaattatg tgaaagtatt agaagtaatc
    18781 cagcaaacga aacaagatga aataattcaa caaataccat tcaataattt cacaggggaa
    18841 aatgagacac taagtttcca tgtatcagaa ttagcagaac gaatggtgtt tcgcttcgcg
    18901 cagcaaccta gctttatcga attcagcgca gaagctgctg gagaaaaact cacatttatc
    18961 gattcaaatg ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg taaagattta
    19021 ccagagttct ttttaacgga tattgcaggg aaagaagtga ctatccattg tcactatcgt
    19081 tttatcggag aattgacacc aacaatgaaa gaattgctgg aaacaatccg tccaatggct
    19141 gaagttacaa agcagctttc tgagaaaaat gatgaattag aaagagaaaa tcattatctt
    19201 attgagaata atacaagatt agatcagacg ttgaaaacaa ccataaatcg ctattgcacg
    19261 ttgttggaaa gtgatgaatg gacaatcaaa catagattag ggcgacgtaa gaaagaaaca
    19321 tcgaaaaaaa tccaagaaaa agagctttct ctatgtattg acgaaaaaat ctgggatgca
    19381 gaaacaaaga tattgaaaat catcgggtgg ggaatcgcta attcggatcg ccaaccattg
    19441 tcctataagt tatcagccga ccaatcacca ttttttgaag caattcctgt tttacgagaa
    19501 gaagtcaatc aagcaaaaca gctagctgaa ggagcaaaag caggatttga actgcggatc
    19561 ctttgcgaga aggaacgttc attcttagtt gaagcagtgg ctcaaaatgg tcaatcatgg
    19621 atcattgaaa tgtaaataat gattaactta taaagataag gagtaaacga atcgtgcaaa
    19681 aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga acgggccact ggcgatttgc
    19741 ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat ccctacgata aaggtagaaa
    19801 aagaaaatgt gattgctgaa gcaacacatg tcgtaagatt ggatatcaat catttatata
    19861 atcttgatgt taagacccaa agcggtttta aggtacgttt atcagggaaa atgagaggaa
    19921 aagcaatctt agacttccaa acagcaagac atcaaaatgg catagctgtg aaattaaacg
    19981 gtaaatatcc ttatgatgac ggcattgaat cttcatggga aagaaaaaaa agattactga
    20041 aaaaagggat aaattatgca aggactcatg gcgtaaaaaa agcaatccgc cgggtcaaac
    20101 tggaattgaa accaggatcg atcgactatg cagagtggat cagtcgtcat gagaaacctg
    20161 atctgaaaga gcaaaggaaa gaagtacaag gatttgccta ccgcccattg atttccatac
    20221 ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat tcggtacttg
    20281 atcaaacgta tgatcactgg gaactatgta tcagtgatga tgcttctact gatccagcta
    20341 tccgaaagtg tctagaatcc tatcaagcaa aagatgatag aattaaagtc gttttccgac
    20401 aagaaaatgg acacatcagt ctggcaacta attctgcatt ggaaatggca gaaggagagt
    20461 ttatagcgct cttagataat gatgacgaat tacctccatt tgccttatat gaagtggcaa
    20521 aagtgctaaa cgtacatcca gaactggatt tgatctacag tgacgaagat aaaatcgatg
    20581 cagatgggaa tcgctttgac ccgcacttca aagcagactg gtctcccgat acattgatgg
    20641 gaaataatta tatctctcac ttaggcgtat atcggacaag cattgttaag gaacttggcg
    20701 gtttccgtaa aggatatgaa ggctctcaag attatgattt ggttcttcgt gtgacagaac
    20761 aaattcctgc agaacacatt taccacattg accgtgtctt gtatcattgg cggacgatcc
    20821 cgggatcaac tgcaagcaat ggagaagcta aaagctacat ctatgattct ggagtgaaag
    20881 cattgacaga tgctctatct agaagaaata tcaagggaag cgtacatcct ggcagaattt
    20941 ctggttttta tgaaatcact tatgatgttt tgcaagaaga cttggtcagt gtgatcattc
    21001 caactaagaa tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt
    21061 cttatccaaa ttatgaaatc attattgcag ataatggaag tacagatcct aaaatgcaag
    21121 aattatttgc agaatacaag catcagctga aagaccgatt tatcgtcgaa ttaattgaca
    21181 ttccattcaa ttattcacgt atcaacaatc tggcagcaga aaaagcaaac ggcaaatact
    21241 ttttattctt gaataacgat acagaagtga ttgaacctga ctggatgaca gcgatggttt
    21301 cttatgcaca gtttgatcga atcggctgtg taggagcaaa attattttat ccggatgata
    21361 caacgcagca tgcaggtgta ttattaggga tcggtggcgt tgcaggacat gcattgaata
    21421 actatgatcg aacacattgt ggttactttg gccgattggt cattgacgtc aattacttag
    21481 cagtcactgc agcttgtatg atggtcaagg cagctgactt caatgctgtc aatggatttg
    21541 atgaaatatt agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg
    21601 gtcgttacaa tgtctatgca catcaagcag aattgtacca tttcgaatct aaatcaagag
    21661 gttatgaaga tacaccagaa aaacaaagac gttttgctgg ggaaatcaaa aaaatgcaag
    21721 ataagtggcc ttcttatatt gctcatgacc cattttataa cgataatcta actaaagaag
    21781 gaatcggcga tttttcgttg agacctgatt gattaaaaga actgtatttt catttttatt
    21841 aatgaaaata cagttctttt aatttaatt
//
LOCUS       DAPBBG010000049        21367 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPBBG010000049 DAPBBG010000000
VERSION     DAPBBG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.049"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            358..1404
                     /gene="gmd"
                     /locus_tag="RD477_002075"
     CDS             358..1404
                     /gene="gmd"
                     /locus_tag="RD477_002075"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDT7634429.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1484..2497
                     /locus_tag="RD477_002076"
     CDS             1484..2497
                     /locus_tag="RD477_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634430.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2534..3478
                     /locus_tag="RD477_002077"
     CDS             2534..3478
                     /locus_tag="RD477_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDT7634431.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3546..4859
                     /locus_tag="RD477_002078"
     CDS             3546..4859
                     /locus_tag="RD477_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDT7634432.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4943..5845
                     /locus_tag="RD477_002079"
     CDS             4943..5845
                     /locus_tag="RD477_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDT7634433.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5850..7502
                     /locus_tag="RD477_002080"
     CDS             5850..7502
                     /locus_tag="RD477_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDT7634434.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7526..8392
                     /locus_tag="RD477_002081"
     CDS             7526..8392
                     /locus_tag="RD477_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634435.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8434..8625)
                     /locus_tag="RD477_002082"
     CDS             complement(8434..8625)
                     /locus_tag="RD477_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634436.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8655..9194
                     /locus_tag="RD477_002083"
     CDS             8655..9194
                     /locus_tag="RD477_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634437.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9322..9675
                     /locus_tag="RD477_002084"
     CDS             9322..9675
                     /locus_tag="RD477_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634438.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            10143..10619
                     /locus_tag="RD477_002085"
                     /pseudo
     CDS             10143..10619
                     /locus_tag="RD477_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10619..10813
                     /locus_tag="RD477_002086"
     CDS             10619..10813
                     /locus_tag="RD477_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634439.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10819..11104
                     /locus_tag="RD477_002087"
                     /pseudo
     CDS             10819..11104
                     /locus_tag="RD477_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            11118..11484
                     /locus_tag="RD477_002088"
                     /pseudo
     CDS             11118..11484
                     /locus_tag="RD477_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            11698..12096
                     /gene="tnpA"
                     /locus_tag="RD477_002089"
                     /pseudo
     CDS             11698..12096
                     /gene="tnpA"
                     /locus_tag="RD477_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12257..12478
                     /locus_tag="RD477_002090"
     CDS             12257..12478
                     /locus_tag="RD477_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7634440.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12788..14015)
                     /locus_tag="RD477_002091"
                     /pseudo
     CDS             complement(12788..14015)
                     /locus_tag="RD477_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(14119..15069)
                     /gene="yidC"
                     /locus_tag="RD477_002092"
     CDS             complement(14119..15069)
                     /gene="yidC"
                     /locus_tag="RD477_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDT7634441.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            complement(15785..16060)
                     /locus_tag="RD477_002093"
     CDS             complement(15785..16060)
                     /locus_tag="RD477_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDT7634442.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            16167..16931
                     /locus_tag="RD477_002094"
     CDS             16167..16931
                     /locus_tag="RD477_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDT7634443.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            17054..17557
                     /locus_tag="RD477_002095"
     CDS             17054..17557
                     /locus_tag="RD477_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7634444.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            17965..18611
                     /locus_tag="RD477_002096"
                     /pseudo
     CDS             17965..18611
                     /locus_tag="RD477_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            18774..20042
                     /locus_tag="RD477_002097"
     CDS             18774..20042
                     /locus_tag="RD477_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDT7634445.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(20069..21268)
                     /locus_tag="RD477_002098"
                     /pseudo
     CDS             complement(20069..21268)
                     /locus_tag="RD477_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
BASE COUNT         6382 a         4109 c         4906 g         5970 t
ORIGIN      
        1 caccggttgg ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat
       61 gtgctaacgc tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca
      121 tccgaggagg ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct
      181 tataaaattt gtcaaaaaaa ctgttgccat tccaattcac gaaaaactaa tattgtgcta
      241 atgcgtgcgt agattgttct atgaaatatc gaaaattatc gtgtattctt gaaaagtatt
      301 agaaattacg agccatttat atggccttat cacttacata ggaaaggata atgtatcatg
      361 aaaaaagcgc tgattactgg tattactggt caggacggtt cctatctggc agatttcctg
      421 ctagagaagg gctacgaagt tcacggtatc actcgccgtg cttctatttc caataccgct
      481 cgcattgacc atttgatggg caagatcacc cttcatgacg gtgacctgtc cgactcatct
      541 tctctgattc gtatcatcag cattgttcag cctgatgaaa tctataacct cgctgcacag
      601 agccatgttc aggtttcttt tgatgttcct gagtactccg gcgatgttga tgctttgggc
      661 gttatgcgta ttctggaagc ttgccgtatt ctgggcctga cgaagaagac taagatttat
      721 caggcatcta cttctgagct gtatggcaag gttgaagaag ttcctcagcg tgaaaccact
      781 ccgtttcatc cttacagccc ttatgctgtt gcaaagcagt atggcttctg gatcactaag
      841 gaatatcgtg aagcttatgg catgttcgct gtcaacggta ttctgttcaa ccatgaatcc
      901 gaacgtcgcg gtgagaattt cgtcacccgt aagatcaccc tcgctgccgg ccgcattgca
      961 gaaggcctgc aggaccatct ggaactgggc aacatggact ccctgcgtga ctggggctat
     1021 gcaaaggact atgttgagtg catgtggatg attctccagc atgagactcc cgaggacttc
     1081 gtgatcgcta ccggcgagca gcacactgtt cgtgacttca ccgagaaggc attcgcagca
     1141 aatggcatga ccattcgttg ggaaggcact ggcattgatg aaaagggcta cgatgttgaa
     1201 accggcaaga tgctcgtttg tgtaaatcca cagtggttcc gtccgactga cgttgacaac
     1261 ctgtggggcg acccgactaa ggcaaagacc gttctcggtt ggaatccgca gaagacctct
     1321 tacgaggaac tggttaacat catggcaaag catgaccgtg aattggcaaa gcgcgaagct
     1381 gcaatgaaga acgcagtcaa gtaagctgac attgaataaa catatctgtt tctatctttc
     1441 cagtcacggc ttcggacata tcgcgcggaa tctgcccatt attatggcaa cactctgccg
     1501 atgcgaagcg aagattacac tggtttgcgg cgcaaatcat ctggattttg caaagcgcaa
     1561 tttggagcag gaactgccca aagagctgat agaccgcgtg cgctatcgtc caataaacac
     1621 agatatcggt ctgattaacc gtgagggcac cctactggtg gacacgggta agctgaccga
     1681 gatctgtgaa tattttttgg caaaggttcc ttctttggca aagacagaag ccgaatggct
     1741 caagcgagaa gctgtcagcg cagtgctttg cgatatgcca atctgggcaa tggatgcctg
     1801 cgatatggca ggagtacctt tcctgtatgt aggaaacttc acttggacag agctgtatcg
     1861 cgaattttta cccgaacata tttggaaaga atatgccaag cgttacggta aaattcagca
     1921 caccatgctt tatgcgctaa ataacgaaga aatgttggaa tttctccaaa acagcgaaaa
     1981 aacaccgact tccgttgtgg cgcgtccgat tcataacgac gaagtgcaac gcattaaatc
     2041 acagcatgaa aaaccgcctg tatttgtcgc actcggtatg tccgcgcggt tcaaacagcc
     2101 gattgatgtc agtcacgcgc cgtatgattt cattacaacg ctaggtgttc cgcttgtggg
     2161 agaaaacgta accgttattt ctaacagcac ccccaataca caggactata tagcggcggc
     2221 agattacgtt attacaaagg ctggatgggg aactgttgcg gagtgtattc tcgcacagcg
     2281 tccgatggca ctgttcgccc gcgatacagt tttggaagat agaacaacta tttgtaaatt
     2341 gcaaaaactg ggtctggcaa tttccgtcac accggaaaac ctccaccgcg tagagcgggt
     2401 gattgaggac ctcaaacagc tagacacctc aaattttgat acattttatg atgctgcgag
     2461 cgaaatcgca gacaaattga tttctctcat gcaatgagag atgctctaaa tttagaaagg
     2521 agcaagcatt attatgatgg aaaaagacgc aaaaatctac gtagccggtc atcgtggcat
     2581 ggctggttcc gcaatcgtgc gcgagctgaa ccgtcaggac tacaacaata ttatcacccg
     2641 cacccataag gagcttgacc tgtgccgtca ggacgctgtt gaagcatttt tcgcacagga
     2701 aaagccggat tatgtatttc tcgctgctgc aaaggttggc ggtattatcg caaaccagaa
     2761 cgctctggca gacttcatgt atgaaaacat gattctggaa atgaacgtca tcaactccgc
     2821 atgggagaat ggctgtaaga agttacagtt cctcggttct tcctgtatct atccgcgtat
     2881 ggcaccgcag ccgatgccgg aaagctgtct gctgacaagt gagctggaaa agaccaacga
     2941 ggcttatgca ctggcaaaga tttctggcct gaagtactgt gagtttctaa acaagcagta
     3001 cggtactgac tatatctctg ttatgcctac taacctgtat ggacctaacg acaactatca
     3061 tcctactcac agtcatgtgc tccccgcttt gattcgtcgc ttccatgagg ctaaggaagc
     3121 aggtctgcct actgttacct gctggggtga tggttctcct ctgcgcgaat tcctatatgt
     3181 tgacgatctg gcaaacctgt gtgtgttcct gatgaataac tatagcggtg acgagactgt
     3241 taatgcaggt actggcaagg aactgtctat caaggaactg accgagatgg tagcaaaggt
     3301 tattggttac gaaggcgaga ttctgtggga taccagcaag ccgaacggta cacctcgtaa
     3361 gctgctggat gtttccaagg caaccaagct gggatggaca tataagactg agttggaaga
     3421 tggcatccgt ctgtcctacg aggacttcct gaataacccg atgcgtgctg aacgctaatt
     3481 taccgaggtt gctgtgattt ttgaggattg cagcagcctc tttcttataa aaaggagaga
     3541 tcattatgaa tattgtttta ctgtctggcg gttctggtaa gcgcctttgg ccgctgtcta
     3601 atgacatccg ttccaagcag ttcattaaga ttttcaaaac tgaagatgga tatgaatcca
     3661 tggttcagcg tgtctaccgc cagattaaga aggtagataa cgacgcaact gttacgattg
     3721 caacctctaa gacacaggtt tctgctatcc ataaccaact tggtgaggat gtgggtgtat
     3781 ctgtagagcc ttgtcgccgc gataccttcc cggcaatcgc attggctacc gcttatctgg
     3841 tagatgtgat gggcattgat ccagaagagt ccgtagttgt ttgcccggtt gacccttacg
     3901 tagaagatga ctattttgag gccctgaaat ctctgtccga tcaggctgac aaaggcgaag
     3961 ctaatctggt gctcatgggt attgaaccga cttatccttc cgagaagtac ggctacatca
     4021 ttcctgagag cgcagagcag actgctttcg taaatacctt caaggaaaaa ccttctgagg
     4081 ctgtggcaaa ggaatatatc gcacagggtg cactgtggaa cggtggtgtg tttgtctaca
     4141 agctgaagta tgtcatggag cgtgcacatc agctgattga ctttgtagat tatcaggatc
     4201 tgttcaacaa atacgatact ctgactaaga tttccttcga ctatgccgtt gttgagcatg
     4261 aggacaagat tcaggtacag cgttttgctg gtatgtggaa ggatctgggg acttggaata
     4321 ctctgaccga aggtatggag gaatccatta tcggtaaagg tgttatgaat gagaattgcg
     4381 atggcgtaca catcctgaac gaactggatg ttcctgtgtt ggctatgggt ctgcatgatg
     4441 tggtcatctg tgcatctcgg gaaggtattc tggtttccga taaggaacag tccagctata
     4501 ttaagccttt cgttgacaag ttcgagcagc agatcatgtt cgcagagaag tcttggggta
     4561 gttataaggt tatggatgta gaagcagaga gcatgaccat taaggttact ctgaatgcta
     4621 gtcactccat gaattatcac agccacaaga accgtgacga ggtgtgggtg gttctggccg
     4681 gtactggtaa gaccatcgtg gatggatatg agcaggttgt taaggctggc gatgtgatca
     4741 ccatgagtgc tggctgtcgg cataccatta tggcagatac tgagctgaag ctgattgaag
     4801 tacagcttgg caaggatatc aatgttaaag ataaacagaa atttgagtta gagtattaaa
     4861 aaacggagga gggctcgtta tacagaaacc gtttttattg aagccttccg caaaggatta
     4921 tctgtggggg ggaaccgcct gaatgatgaa tttgcaaaag gaattgagat gtctcctctt
     4981 gcggaaactt ggaatgctct acgcacccgg atggaccgag ctttgttgtg tctggtcaga
     5041 gtatcagggg atgaccttga cggaggtgtt gcaagaacac ccagagtttc tgggaacgca
     5101 tccgcagacc aaaggagaat tgccgatcct gatcaaattc attgatgcga aaaaggattt
     5161 gtctgttcag gtacatccca cagatgaata tgcaattgaa aaagaaaacg ggcagcttgg
     5221 aaagacagag atgtggtatg tactggatgc cagcttaatc tatgggctgc agaaaataac
     5281 agaagaagct gtgatccgtg aagcgattgc tcagggaact gttgagcgtt atcttcagaa
     5341 ggttccggtc aaaaagaatg atgtgttctt tattgaggcg ggaacgattc atgcgattgg
     5401 tgcgggtgca ctaattgcag aaattcagga aaattccaat ctgacttatc gtctctacga
     5461 ttatgaccgt gttgacaaaa atggtaaaaa gagagaactt catgtagaca aagcgctgga
     5521 agtagcgaac ttgaaagggt tgtcagaacc gaaacagccg cttcgtgttc taaaatatcg
     5581 tgctggtgta gctgcggaat tgctttgcag atgcaagcat ttcgaggtct ctcgtatgat
     5641 tgtcaacact gagagaagac agacggtaca ctatcgtgcg gattcaatca gcttccgtgt
     5701 gttgctgtgt gtcaatggct gtggtaccgt tcgatttgat aatgaatcgt tggacatcta
     5761 taagggggac tgcctttttg tgccggccga ttctactgaa atcagcattc acgggtcgat
     5821 tcagtttctg gatgtgagag gataataata tgaattataa agtagaatat gagcgttggc
     5881 tgaatcatgc caatgcagat attatagaag aactgcgttc ctattctgag aaggacattg
     5941 aggactcttt ctataaaaat ttgtctttcg gtactggtgg tctgcgtggt accatcggtg
     6001 caggcactaa tcgcatgaac gtacacaccg taggtaaggc aagccaaggt ctggcagact
     6061 atctgaataa gaactatgaa gccgcttccg tggtggttgg ctatgattcc cgtatcaaat
     6121 ctgatgtgtt tgcaaagact gccgcagaca tctttgctgc aaatggtatt caggtgcact
     6181 tgtggccaga actgcttcct gttcctacag tatcttttgc gactcgttat ctgaaggcag
     6241 atgccggtgt tatgattacg gcatctcata acccttctaa atataacggc tacaaggtat
     6301 acggtgcaga cggctgtcag attactaccg aggctgctac tgcaattctg gcagaaatcg
     6361 aaaagctgga catctttgct gatgtaaaga tggatggcga tgcttctaag attgcatcca
     6421 ttcagtcgga agttctgact gctttcatcg aagaagtgaa ggggcagtcc gtcctgtttg
     6481 gcgaagaagt ggacaagaat gttgctatcg tgtattctcc tctgaatggt accggtctgg
     6541 tgcctgttac ccgcaccctg gcagagatgg gatacagcaa tgtgaccgtt gtggaagaac
     6601 agcgtctgcc ggacggtaat ttcccaactt gtccttatcc gaaccctgaa attaaggaag
     6661 ctatgtctct gggtatggag tatgccaaga agtacaatgc ggatctgctg ttggccactg
     6721 accctgactg cgaccgtgtt ggtatcgcag ttaagagcgc acatggtgac tatgttctgt
     6781 tgtctggtaa tgagactggt ctgctacttc tggactacat ctgttctcag agaattaagc
     6841 atggaaaaat gcctgctgac cctgtgatgg tcaagaccat cgtcaccatg gatttgggtg
     6901 aacgtatcgc atccaactat ggtgttcgta ccatcgatgt gctgaccggc tttaagttta
     6961 tcggtgagca gattggtatg ttggaaaagg ctggtaaggt tgacagctat atcttcggct
     7021 ttgaggaatc ttacggctat ctgtccggta gctatgtccg tgacaaggac ggtgttaacg
     7081 ctgcttatct aatctgtgaa atgttctctt actacaagac tcagggtatc agcctgctgg
     7141 ataagctgaa cgagatttat acaaagtatg gctattgttt gaacaccctg cattccttcg
     7201 agtttgacgg tgcggcaggt tttaccaaga tgcaggaaat tatggttgaa ttccacaagg
     7261 gtctcgatat aattggtggt aagaaggtta ttgaaacttt agactacagt aagggcctga
     7321 atggtctgcc gaagtctgat gttttgaagt ttatgctgga agataactgc tctgttgtgg
     7381 ttcgtccttc tggtactgag ccgaagctga agacctatat cagcgtcagc gcagagaaca
     7441 aggaagccgc cgaagctatg gaagctatta tggctgctga actggcggaa atgttcgagt
     7501 aagaaaagaa aggagaaatc ttgtaatgga ggaactgttt tctaacgctt tgaaatcggt
     7561 gaagtcaaat cgtgtgctgt acacggcatc gttctttgca agatcctctc ttttgtattt
     7621 gcaggaaatt ggacagctga ctgctctgaa ggcacataca tctaggcgta caggattgtc
     7681 atcttacctt tgtttcctgg tagaagttgg ttcaggcaca ttggagtata aggggcagaa
     7741 atatacgctt caagtaggag atatggtgtt tattgactgt ttgaagccgt atgtccatga
     7801 gtcatcagaa gatgatttgt ggactttgcg gtggtgccat tttaatggca ttggtatgac
     7861 ggaagtctac aaaaaatacc ttgaacgagg tggtcaaccg gtctttaaaa catcaaaaac
     7921 agataagtat gctgacttgc tgaggagcct tcatatggtg gcagagggac aggagtatgt
     7981 ccgggatatg aaaatcaatg ttctactcag tgaactgctg atgcatttga tggaagattc
     8041 ttggaatcct gacaaagtgc cgaatttctc tacaaaaaaa atggaattgc agaacataaa
     8101 acaatatctg gatgaccatt acacggagaa gattacgctg gatcagttga gtgcagagtt
     8161 ttttatcaat aaacagtact tagctaagat ttttcgtgaa acttacggta caactgttgt
     8221 cggttatctg aatcagaaac gaatcacaca ggccaagcac ttgctacgct ttacaaatct
     8281 aagtatggaa gagattgcaa caaaaattgg cataacagat gcaaactatt tttccagaac
     8341 ttttttcaag gtggaaggtg ttcgtcccag cgaattccgc aagatgtggt aataaaaagc
     8401 cggcggtgtt gagccgccgg tcagtacctg tcgttaaccc gcattcaaag ctttaatctc
     8461 agctttgatt tcagcacgaa cacgatagta atcttcagtt tcgatttcgg tatccagccg
     8521 agttgctgtc ttttcgcagt caggacaacg ctctggctgt tcgtcggcag ggaagctgta
     8581 gtggcaggaa tcacaggtgt aaatgtgatc gtcacgtata atcatcacct accaatccag
     8641 tgcttagatt tttcatgcaa ttactattat atcgaattgg tgattccaca aaaatccgct
     8701 tcttgataaa tgtacgattc agagtgatat atagtactgt actgccaaag ggaataccac
     8761 aacggcagaa atggatgatt cacatggaag aaattatgca gatgatttta gggaacgcaa
     8821 aattccagct cggacaggtg gtttcgaccc ctgctgtatt ggaaactttc aaccccctgc
     8881 agaccgcaaa cggcattccc gaagccttga gcaacctttg gtttagacag gaaattagta
     8941 ttgcggagat cgaggacgca gttctggatg ccaagaacgg caaggagcta gcaaacagcc
     9001 tcaaccgcat caagcttttc cgcaagttca ccctcgaccg agagctggac ggcatggtca
     9061 gactgaaggg cacggacgtc tggaagaaca tcagctacct caaggtctac accgccacgc
     9121 cgcctaagaa accgaccccc ccaaggttcc agtcgaacgg ctggagcctt ttcttctctc
     9181 ataaatcggc ataatgtata caaatacagc tccaaatgat tgtgtagtat atgcctgtgg
     9241 attgacttga tatagtcgag agactatggc aatatacaca caaccaaagg ggaaaatccc
     9301 aaaaacaagg aggaaacgct aatggcaatg agcgaaaagg caaagaaata tttgaaggag
     9361 atcaagggtg caaagaccat cccggagctg aaggatgtgg agatcgcaat caagcgagac
     9421 ggtatccttg cttgggcaga gttcaccaag ttgaacgagg cggtggaaga gaagaaggtc
     9481 gcccttcgca agaaaaagca ggagaccagc cttcaggaga tccttttctg ggcctacaaa
     9541 aaggaatcag acaagctgct gaagatgatg gacgagggag cagacaagga tgccatccag
     9601 atgcaggtcc agcgttacga cagcatcggc cagatcattg cagaggcaga cctcgaagat
     9661 gagtatgagg tttgagaata cagcctgaac cactaagaac caaataccga aagggacttc
     9721 tgaaacagac agaggtccct ttcttttgct cactttggcg gtcaattatg aaccaaaaac
     9781 cagtcgaatg attgtgtaca ttatggcttt caaacgactt gataataatc acttttagag
     9841 tgatatatgt actacaaaaa aacaaaagac tggagaaaac aacagatgag cgaatacaga
     9901 atcatttata tcagcgacga ggacggcggc gaggtggaag gatgtggcta cacggctacg
     9961 atggttatga tgcggtcaga aacttcttgg aatttgcctg cggcatcttg aaaatcctgc
    10021 gggtgcgatt catcagaaga gtggactgaa atacaagtta gcagatagag aagagagtca
    10081 ggtttcgact tggctctgtt ctctgtctac tggcagaaat acattgaagg gaggtgacga
    10141 ctgtggcaac cagaggaaga aaatcaaccc cgacagccat gaagaagctg gagggaaatc
    10201 cgggcaatcg cccactcaat gcaaaagagt caacgccgga aaagaaggca ccgacctgtc
    10261 cgaagtggct ggaaccagag gcaaagaagg aatggaagcg tctggcaaag cagatggagg
    10321 ccatcggaat cttgacagat gtggatatgg ctgcctttgc cggttagtgc caagcctacg
    10381 cccgctggaa ggaagccgag gaattcatta cccagcacgg tatcatcgtg aaaaccctat
    10441 ccggctattg gcagcaggta ccacaggtgt ttattgccca gacctatctg aaaattatga
    10501 accgattttc cgagcagttc ggtctgacgc cttcgtccag aagccgtatc attgccggtg
    10561 atggcggtgg tgtcggagca gaagatgcga tggaggctct gttgggaggt ggtagctgat
    10621 ggcagaaacc agaccaaagg gctatcccaa attaaaggaa tacacaccaa gccgattcat
    10681 gctttcggaa tgtcactacg acaaagccag agcggatcgg gcggtgaact ttatcggaca
    10741 gctccgacac accaaaggca aatgggcagg caaccgcttc tggctcttgc cttggcagga
    10801 acagatcatc tagtgtaatc tcgcttaact gtattaacgg acgtagcgac tttattaaga
    10861 gtgttcatgg taggagcatt agttgcaaca atgattgata ttaaattttt atggtaccaa
    10921 cgattggcga tgtgacattg aatatgcaaa actatatcga tatgatatta tctaaaatct
    10981 tactcgctgg gatcgtcgag gtgtttattg gttgttagga aagaaaaata tgacccaact
    11041 cgagcgattt ttatcgtatt acttgtgtgt ggctttatct gcattgtgtg tgatttcgaa
    11101 ataagggaga gccaaaaatg agtagaaaat tgatattgat tagtcacgga aaattttgtg
    11161 aggaattgaa aaaaagtaca aaaatgatta tgggacctca agagaatatc tacacgttac
    11221 ctttattacc agaagatggc actgaaacat acagacaaaa gctcttactg tcatagattc
    11281 gttaaatgat tttgtggtac tatgcgatct tttaggcggt acacctacta atatcatttc
    11341 taaactgata atggaaggac aacagattaa actttatgtc ggattaatct tcctatggtc
    11401 attgaattta tcaactcgca agtgattggt tcagagcccg agtttgttcg tgcaggatgt
    11461 gaaagcattg tatctgtcaa ctaattaatt agtggaatag tagaagatga agagtaataa
    11521 aaagagagtg ggacaatagt cagaaaaaaa tctgattatt gccccacttt ttttattgac
    11581 tttagtcagt ttcgttggcg tagcgtacga aacaaaaaaa cgcgcgtttc tacgcgtgga
    11641 cgaaatagct ttagcgataa aagtttagca catacgagat gggattgtaa gtatcacata
    11701 gtctttaccc cgaagtatcg acgaaaagtg atttatggaa agttaagagt aagtattggc
    11761 cagatattaa gaaaattgtg tgagaggaaa gatgttgaaa tcattgaagc tcatgcaatg
    11821 cctgatcaca ttcatatgtt agtaagaata ccgccaaaga ttagtatttc tagttttatg
    11881 ggctacttaa aaggacgaag tgcaacctta atccatgaaa aacatgcaaa tttgaaatat
    11941 aagtatggga ataaaagctt ttggaccaaa ggatactacg tgagtacggt tgggttaaat
    12001 caaaaaacaa ttgaaatata tatttgagaa caagaagcag atgatcgaat tagagacagt
    12061 ataagtaaac aagagtatgt agatccattt aaatagttaa agagagatag ggctgtgagt
    12121 ggtgagccct tctagggctg ttaaaaagcc acccgttttc acggatggat atttactgat
    12181 tttaaggtac acaaaaagca ctcatccctg taggattaaa gtgaccaaac ccaatcagaa
    12241 aggaagcgag tgctttatgt ttaaaaatta taccatcaac caaaaaattt taccattaga
    12301 tatggaacaa tatatttctg aaacagatgt cgcttttgcg gtcaacgctt tagtcgaaga
    12361 aaagataaaa gtggatttat acgtgtcttc aaaatttacg aatgtgaaag tggtcggtgc
    12421 tgacctgttc gtagtcaatg tacgaaagca aaaagtgagc gaaaccgaca aattttagtg
    12481 aataatacat ggcgttattt caaagcagaa tgtaaaaaaa cttttagagg aaaaagaagg
    12541 ttcgatctat cggaaacgga tttccactta tgaaggaaac aaggagccaa gattgacatt
    12601 ggcttggcac tcatggcgtt aaattaagaa aattaggaaa atacatggag aggaaaagac
    12661 gtaaagaaga aaaaactatt gtcccactct ctaagactgt agacaaaagc atcagtttcc
    12721 tctttactaa gagtgtagac tgatactttt gtctattttt gtataataaa agatataacg
    12781 acatatgctc ccactgccaa gtagctaatt ttttcagatt cattgcgaca aaaacaagca
    12841 ttgtatgcac ctgatttttt ccaagcctcg atacttggtg tagcgcattc catgtttttc
    12901 ctttgcgtct gcaaaaatgc gttcaatcgt ttcttttctt tttttatatt tttcacgatt
    12961 gtacaccgta tggcgtaaat gttctgcttc atctaagtag tcttcccaaa tatgtcgatg
    13021 tatttctttt tggtgttttt ggctttctgt acaatttttc aataatggac aactttcaca
    13081 aataaaagat ttagatacat attttttata gccatctcga gtagttgtgc gaaactgata
    13141 taagggagaa tgggtcgtag ttttcgttcg attaaatact tgacgttcca aggtgttttg
    13201 tacgcagcat tagcagctac accataacgt gatttaacgc gttcgatttg tggcacgagc
    13261 attcggctat catgtagatt tccaggcgta atcatgacat ctaatacaaa accattttca
    13321 tcaccaacgg tatgagcaga ataagcaaac tgtttttctc gttctgtttt tacataataa
    13381 ccactttctg gatctgcgat gctaaatttt ctttcgacca cctcctgacg aatgattgaa
    13441 ccaaaaggac gtttgccttc ggcaattctt gcctcattga tttcttcttc taattgtttt
    13501 tggtataaaa tggcttcttt atgtgccata tctcttgtaa aaatacgttt gttcgcattc
    13561 gctttaatat gagttgaatc catatacaaa atttcttcat gaataaatct agcatcaact
    13621 gcttattcga gaataatgat aaatatctgt tcaaacaaat cggtttctct gaaacgccgt
    13681 gcatagtttt taccaaaagt tgaaaaatga ggaactttat caattaaact aagcccaaga
    13741 aaccaacgat aagctaggtt tgtttcaact tatttaatgg tctgacgcat cgaacaaatt
    13801 ccaaataaat attgaatgaa tactaatttt actaagacga caggatcaac gctagggcga
    13861 ccaatggtcg agtaagttga ttcaacaatg agataaataa aatcaaatga caataatttg
    13921 tcaattttac ggactaaatg atttttagga acaagtgcat ctagcgaaat tttgtcttct
    13981 tccattcgac tatacattga ttatttggac atcattatac attcctcctc gaaatgcgaa
    14041 cgttttcttt aattgtaaca aaaaaactgt agaacaaaga aattttttta tttgtctaca
    14101 gttttttgtc atatattttt atttccgctt ctgctgcctt cctgcgttgc ggcctttgtt
    14161 gtttgtactg ttttttggtt gtttgatttg ttttgttgct tgtttgttgt cagctggctt
    14221 tgctgattct gtcacgtctt ttcttggagt aacgacttgt tttggcgggt tttgtttcaa
    14281 ctcttctgcc acttgtgctt tcaaacgcgg tttcattata acgtttgtga tgaatgtttg
    14341 cagacagctg aacacaccac cgactaccca gtaaagtgta acaccggctg gcgcactgat
    14401 cgacatgaag acgatcatag caggagagac gatcagcatc gtacgcatcg tctttttctg
    14461 ttcttcagga ataccgattg ttgaaatata tccttgaagc agataagcgc cccctgcgat
    14521 ggccacaagc accatacttg gtgagcctag atcgatgccg taaaaagatg attcacggat
    14581 accttctgtg tagcgagccg taaaatacaa ggcagagaag atcggcattt ggatcaatag
    14641 aggcagacag ccgattccgc cggtcatact caatccattt tcgcgatata cttgctgcat
    14701 ttctgcttgt gctgccattt gttcttcacg tgttgttgca gcttttagct tttgttgtgc
    14761 tgcttcaact tgtggtttta acgcttgcat tttttcagat tggatcattg tttttttcga
    14821 ttgactgata cctagtggca agatgatgat acgaacgatc acagtcattg caataactgc
    14881 ccagccgtaa gaccaattga aattctcaac tagatatgta atcgcttggc ccataggatg
    14941 gacgagtact cgataaacga ttcctgaagt gtcagggttt ccgtttgaat ccatccgcac
    15001 acagccagat aaaaagagca ggatactgac aagtccggat cccaataacc agtttttcca
    15061 tttattcata gaatacttcc tcttctataa tttttacaca aacttaacta tactaaaaaa
    15121 gcgcatggaa ttcaatcaac ttcatagaaa tttcatataa atattcgttc tttttcgtag
    15181 cgaaaaattt tgaaaaaaga caaaaaatca agaaagaaat acttagaaag aaaagagtga
    15241 ttccaagtgt ttctttctta tttaaaaaac taaaaacttt tttgttctgt ctggaaaata
    15301 tgggttaagc aaatttattg caagttatag atcagctttt ttactacctg tctttaatac
    15361 ttgaggcaaa gtgcctcaaa aacaacctta tcgacgatgt tcaaaagtca gtttcttggt
    15421 attaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt ccttaatact
    15481 caaaactaag cgactttttc acaaccagtc cttaatgctt gatgcagatc agctttttcg
    15541 caaccttatc gacgatgttc aaaagcttgc atctacggca ttaagtccag cctgttgaaa
    15601 aatatggaga gctatttttc acaaccagtc cttaatgctt gatgcagacc agctttttca
    15661 caaccttatt gacggtgttt tatcagctgc ctctcggtat taagtcatta catttcaaaa
    15721 tatgagaagc tatttggaaa atgtttctcc ttaatactcg aggcaaagcg cttcaaaaac
    15781 aaccttatcc caccacatca aatttctttc gttcctcaat catcggatct tcttggacat
    15841 ccacatactg cactcgtcca cttgggctcg gagacttctt gacttcttcg ataaattttt
    15901 ggatgatgtc gtcatttccg attgcttcga ttgtgactga gccgtcgtct tcgtttttta
    15961 ccgttccggt cacgcctaac tgatcagcaa ccatcttggt catatagcga aatcccacgc
    16021 cctggacacg tccttggaca ttcattttaa ctttgcgcat gttcgtcacc tttctttcct
    16081 gttttataaa tcaatgatac cgattttttt aggatgaaac aagctcaagc acctattcca
    16141 tgatataatc agttgaggtg aggaatgtga aagagatcca atcagtaaaa aatccaagaa
    16201 tcaaagaatt aaaaaaacta cataaaaaga aatacagaga agaaaaggca gaatatcttt
    16261 tggaagggtt ccatctgatc gaagaagctg cgtcgtctga tgcagaaata aaagaacttt
    16321 ttgtgacaca gagaggtatg aaagaatggg gagactggct ggctgtaaac aaagagattt
    16381 ctacttttct agtttcagat gaggtcatga ccgctatgtc agatctacct acaccgcaag
    16441 ggatcttagc agtgatgacg ttaacatctg aagatacgcc agatttttca ggaggctggc
    16501 ttttactaga taatgtacag gatcctggaa atgtgggaac gatgatccgt acggcagatg
    16561 cgtttggtat ggctggtgtt ttattaggta aaggaacggc agatatttat agtacaaagg
    16621 tgctgcgaag catgcaggga agcaattatc atttgcctgt gttacaaaaa gatttggcag
    16681 aagtgatccc tgtaatgaaa ggcaaagggt ttacggtgta cggtacagaa ttaaatgaag
    16741 aagcacatgc attagacaaa gtagaaaaaa cagaggattt cgctataatc atgggaaacg
    16801 aaggtcaggg agtaagtcag gaaatcttgt cttcgactga tcagaacgta tatatcccaa
    16861 tgaaaggaaa tgcggaatca ttgaatgtcg gagtcgcagc agggatcgtt ctttatcatt
    16921 tttctttatg attaaggaac tattaagctt tagctatttt atctagagcg actagaataa
    16981 tccgaacact ggtggtatac ttagatcaat ttcagaagaa aatgaaattc aaaaaggtgg
    17041 caaatcgata attatgacag aaaaatggcg tgaagacgaa gaatatatgt cttatgtaca
    17101 agatctttta gaaacagaag cagtgaaaaa attagcgaat tacacacagc atgttcattc
    17161 cactcgtttg gagcattcca tcagtgtttc ttactatagt tatctgctcg caaaaaaatg
    17221 gggtggcaat gctaaagcaa cagcgcgtgc tggacttttg catgatctgt tctactatga
    17281 ttggcgtacg acgaaatttg acgaggggac acatgcgtat atccatccaa gaattgctgt
    17341 aaagaatgca gaaaaaatca ccgatttgtc ggatcttgag cgagatatca ttttgaagca
    17401 catgtggggt gcaacgatcg caccgcctaa gtataaagaa ggatatattg tgacattcgt
    17461 ggacaaatac tgcgcagtca aagaagcggc tcagcccatg tctgcaagta tgcgtaagag
    17521 atggcaacgt tactttggga aagaatcctc gatttaacag gtaaaaccaa taatagacca
    17581 tattgaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt acgaaaatta
    17641 cttctcaaat ttttgtgaag tttcggctta ttaccgcaga tgcaagcttt tgaacaccat
    17701 tcattcgaaa caagggactt tgacacgact tttgtcacag tccttttttc tgtttgcagt
    17761 tgactatctg ttcaagatac ggtttacttt tatttatcga atgaatcatg tacatagaat
    17821 aataagcatt aaagggggtg atgataagat gcaaaacgta tcttatttaa aagagtccag
    17881 gatcttcagg taacacatac tttgcagaag atcacttttt tctacaaagt atgcactttg
    17941 gttagaagaa aggactttaa aatcatggaa caaacaataa aaccatacca atattttttt
    18001 ataataagag aagtagaaca gttattaaat gcgtactcat ctgtcaatga tccaaaaacg
    18061 gtacaaacag ttcaggcact agcagctgaa aagattcgag acatattaga tcacgaactg
    18121 ccagaatcga tacattttta acgacagttt tagacgtaaa actgacaaaa gcgcaagcag
    18181 aaagagcatt ggaggagctg aagcaaatcg ttcagccgtt tatgcagcct agcaaaccac
    18241 aaatcgagaa attattccga aaagtgaaaa aactcaaaca accagcttgg aatgaaatcg
    18301 acttgcaaga acatacgtac atcggctgga atgatccagg tacgcagaaa aaattcatgg
    18361 tctattacga agatgaaaaa ttaaaaggga ttagcggtac tttatcacca acgataaata
    18421 agggaatctg tgcaatctgt cagaaaacat cgaatgtttc gctttttctt tctacaacga
    18481 aagcaggttc tgatggcacg tatacgaaaa aggggaatta tatttgtcat gatagtgacc
    18541 aatgtaatca acagttaacg aaattggctc ctttctatga atttgctgaa cgtgtaaaga
    18601 aagaacgata attttattgt tttttacaga aaaatatgct atcttttaat ggaagtgaat
    18661 aggtgatggt gttcgccttt aaatgctaac gaaagatagc taatgacgcc tactaaagtc
    18721 tggggacagc ccctttcttt agtaggcgtt tttttattga ggaggaattt tttatggaaa
    18781 aacaattacc gagtccagta cgcattttag tgtatatgct gaagtggctg gtgatcacag
    18841 cgattctcgg tatattcatg gggagcttgt ctgctttttt cttaaatagc ctgacagttg
    18901 tgacagatat ccgactatca catccttggc tcctttgtct actaccagtt agtggtgcgc
    18961 tttttgctta tctctatgcg tatcacggcg gactttctag tcgtggaaac aacttagtga
    19021 tcgatcaagg aaatggtgga gaagaaaaaa tcccgttgcg gcttattcca cttacactgt
    19081 ttggaaccat aactacccat ttatttgggg gatctgttgg ccgtgaagga acagcagtcc
    19141 aaatgggggg agcgttagca gataatacgg cacgtttgtt ccgattggat aaatcagaac
    19201 gggaaatctt gattatcagt ggaatcagtg cgggttttag ttctgtattt ggcacacctt
    19261 tagctggaac attatttgga ttagaagtgt tggcaatcgg gaaagtccga acagaagcga
    19321 tcttccctag cttctttgct gcactgtttg caaatttcat gacagaaagc tatggtgttt
    19381 ctcatctaca ttaccaaatg ggtgtgattc ctgaatggag tatgttgctg ttcttcaaag
    19441 tttttgttgc tgggattgct tttggtttga ttggttgggt attcagccgt tcgatcgtct
    19501 ttttaaaagc gcgatatgca caatggattc ctcatcctgt ttgtcgaaat acgataggtg
    19561 gagctgtagt tgtggcagca gcattgattt tgcaaacaca acgttatctc ggattgagtc
    19621 ttccgttact tcaagaatca tttaatggac aggcacatgt ctatgatttt attgggaagt
    19681 tattcttcac agttttgtct ttaggcgcgg gatatcaagg gggagaagtg acaccattgt
    19741 ttgaaattgg tgcaacgctt ggtgcagcac tagcgccttt gctccatgtt tctgttcctt
    19801 ttcttgcagg attgggcttt atcggagtat tttctggtgc gacaaataca ccgattgcct
    19861 gctttgtgat ggggatcgaa ttgttcggtt cacaagcggc agtatacttc tttatgatct
    19921 gcttgatcag ctttatgtgt tcaggcaaca gcggtattta ttctgctcag caagtagcgg
    19981 tcaaaaaagg gatactcttt ttgccaatag ttaagagaat gaaagaaaaa aacaaggtgt
    20041 gacatatgtt ttgtcacacc ttgttttttt agaaaagtaa agtaatcagc gataagaatg
    20101 cggagcctgc gaaaagcaat ccgcaaatga tcatatgtct ttttttgttc atcatcaaga
    20161 tggcgaggta ttctcttaaa aacgcattag gcaagtagta gaaagctgtt tttgcaccaa
    20221 taccatttgc tttcaatttg gcttgccgcg cataaagacc ggctctaaaa atgtggtaat
    20281 tgttggaact aaagatcgcg cggtatggcc ctttcatttg ctgatccatg atttcctttg
    20341 aatagagcat gttttccaaa gtggttgtcg aattggtttc gacaagaatg tctctttcag
    20401 ggattccttg ttccatggca tattgtttca tcgcaatggc ctctggtact ttttcgtctg
    20461 ctccttgccc gccagacatc aataagatgg gcggatttaa tgtagcacgg ctttgagccc
    20521 gataaaaagc aattgcctta ttgattcgct tggctagaag aggagaaact gtttctccat
    20581 tgatcaatcc agcgcctaac acaacgatga agtcttgatt gtatcgaggc cggttgaact
    20641 gatacaagaa tgagacagtc aaaaagttgt agaatacgat aaatagatag atcaagatca
    20701 ggggaacgca gaaaaacaaa gcatttatcc attgcggcaa atattttaac aagaaaaagt
    20761 caaaaacaag aaacagtgta agaaaaatgg ctagtatcaa cgttaaaaga ttagctaatg
    20821 aatgcgattc tctacgtaaa actgtgaccc cgtttcaaaa aagaaaaaca atcaatccat
    20881 aaataccaaa taaaaggaca agtagcaatg gaatcgtgat caaggcgatc agtccgccaa
    20941 gaaaaatatt ttgcgtttca tacagcaaga caaacaagta gatgccgaaa ctgatgagta
    21001 aaacattgaa taaaagacca ttcagtagcc ttcgtttttc tgtgaaataa gaaaaagcaa
    21061 agatactaaa gaacaacaat ggaatcaaaa aataaaaggt cgaataaaaa atttccaaca
    21121 cgacaaaata agttaagatt accgtaaatg tccacgtact gccgtaaaag aacaaggaag
    21181 gatccttctt ctgttttcgc caataaacga tagagaatac cccaataaga tagagaacta
    21241 aaaagatcag atctctggtt agattcataa attcaactcc tttttcttat acagtataac
    21301 gaaaatagat gaaaaaagct gttatttaaa ctagaaaaag caaaaaaaca tctttccgat
    21361 ggaaaga
//
LOCUS       DAPBBG010000050        20942 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPBBG010000050 DAPBBG010000000
VERSION     DAPBBG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.050"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            168..362
                     /locus_tag="RD477_002099"
     CDS             168..362
                     /locus_tag="RD477_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634446.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="RD477_002100"
     CDS             631..930
                     /locus_tag="RD477_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634447.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="RD477_002101"
     CDS             complement(1027..1461)
                     /locus_tag="RD477_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7634448.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="RD477_002102"
     CDS             complement(1693..2403)
                     /locus_tag="RD477_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDT7634449.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="RD477_002103"
     CDS             complement(2393..3253)
                     /locus_tag="RD477_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDT7634450.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="RD477_002104"
     CDS             complement(3258..3896)
                     /locus_tag="RD477_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDT7634451.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="RD477_002105"
     CDS             complement(3911..4210)
                     /locus_tag="RD477_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7634452.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="RD477_002106"
     CDS             complement(4253..5737)
                     /locus_tag="RD477_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDT7634453.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="RD477_002107"
     CDS             complement(5757..6218)
                     /locus_tag="RD477_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7634454.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="RD477_002108"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="RD477_002108"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDT7634455.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="RD477_002109"
     CDS             7521..8291
                     /locus_tag="RD477_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7634456.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="RD477_002110"
     CDS             complement(8564..9391)
                     /locus_tag="RD477_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7634457.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="RD477_002111"
     CDS             9959..11194
                     /locus_tag="RD477_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDT7634458.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="RD477_002112"
     CDS             complement(12193..12453)
                     /locus_tag="RD477_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDT7634459.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="RD477_002113"
     CDS             complement(12480..13781)
                     /locus_tag="RD477_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634460.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="RD477_002114"
     CDS             complement(14073..14531)
                     /locus_tag="RD477_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7634461.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="RD477_002115"
     CDS             complement(14544..15875)
                     /locus_tag="RD477_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDT7634462.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="RD477_002116"
     CDS             complement(15886..17328)
                     /locus_tag="RD477_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7634463.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="RD477_002117"
     CDS             complement(17315..18079)
                     /locus_tag="RD477_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDT7634464.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="RD477_002118"
     CDS             complement(18183..19058)
                     /locus_tag="RD477_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7634465.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="RD477_002119"
     tRNA            complement(19313..19397)
                     /locus_tag="RD477_002119"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="RD477_002120"
     tRNA            complement(19415..19486)
                     /locus_tag="RD477_002120"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="RD477_002121"
     tRNA            complement(19496..19568)
                     /locus_tag="RD477_002121"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="RD477_002122"
     tRNA            complement(19630..19714)
                     /locus_tag="RD477_002122"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="RD477_002123"
     tRNA            complement(19735..19805)
                     /locus_tag="RD477_002123"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="RD477_002124"
     tRNA            complement(19851..19922)
                     /locus_tag="RD477_002124"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="RD477_002125"
     tRNA            complement(19934..20006)
                     /locus_tag="RD477_002125"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="RD477_002126"
     tRNA            complement(20009..20082)
                     /locus_tag="RD477_002126"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="RD477_002127"
     tRNA            complement(20089..20169)
                     /locus_tag="RD477_002127"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="RD477_002128"
     tRNA            complement(20171..20246)
                     /locus_tag="RD477_002128"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="RD477_002129"
     tRNA            complement(20282..20354)
                     /locus_tag="RD477_002129"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="RD477_002130"
     tRNA            complement(20364..20436)
                     /locus_tag="RD477_002130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="RD477_002131"
     tRNA            complement(20452..20523)
                     /locus_tag="RD477_002131"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="RD477_002132"
     tRNA            complement(20538..20627)
                     /locus_tag="RD477_002132"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="RD477_002133"
     tRNA            complement(20667..20740)
                     /locus_tag="RD477_002133"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="RD477_002134"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="RD477_002134"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6603 a         4392 c         3330 g         6617 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gt
//
LOCUS       DAPBBG010000051        18977 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPBBG010000051 DAPBBG010000000
VERSION     DAPBBG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.052"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(53..451)
                     /locus_tag="RD477_002135"
     CDS             complement(53..451)
                     /locus_tag="RD477_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7634466.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(643..1410)
                     /locus_tag="RD477_002136"
     CDS             complement(643..1410)
                     /locus_tag="RD477_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7634467.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(1445..2668)
                     /locus_tag="RD477_002137"
     CDS             complement(1445..2668)
                     /locus_tag="RD477_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDT7634468.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            3649..4452
                     /locus_tag="RD477_002138"
     CDS             3649..4452
                     /locus_tag="RD477_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7634469.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            5037..5492
                     /locus_tag="RD477_002139"
     CDS             5037..5492
                     /locus_tag="RD477_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634470.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            5611..6669
                     /locus_tag="RD477_002140"
     CDS             5611..6669
                     /locus_tag="RD477_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDT7634471.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            6711..8660
                     /locus_tag="RD477_002141"
     CDS             6711..8660
                     /locus_tag="RD477_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDT7634472.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            8663..12304
                     /locus_tag="RD477_002142"
     CDS             8663..12304
                     /locus_tag="RD477_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634473.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            12704..13042
                     /locus_tag="RD477_002143"
     CDS             12704..13042
                     /locus_tag="RD477_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634474.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            13152..13373
                     /locus_tag="RD477_002144"
     CDS             13152..13373
                     /locus_tag="RD477_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634475.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            13393..15549
                     /locus_tag="RD477_002145"
     CDS             13393..15549
                     /locus_tag="RD477_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634476.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            15561..15794
                     /locus_tag="RD477_002146"
     CDS             15561..15794
                     /locus_tag="RD477_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7634477.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            15784..16650
                     /locus_tag="RD477_002147"
     CDS             15784..16650
                     /locus_tag="RD477_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634478.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            16665..18635
                     /locus_tag="RD477_002148"
     CDS             16665..18635
                     /locus_tag="RD477_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634479.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            18802..>18977
                     /locus_tag="RD477_002149"
     CDS             18802..>18977
                     /locus_tag="RD477_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634480.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         6869 a         2754 c         3620 g         5734 t
ORIGIN      
        1 ccatacgtcc taaaacaagc tgtttttgct taatatgatc atctgagaga catcaaatca
       61 acttttctct caacaaatca ttcaaagctt tcgacagatt aatattctct tcagccgcta
      121 aaacatttaa atagtgttcc acagtcactg attttcgatc ataattttgc ttgtttcgag
      181 ctttagcaac tttcgtatta gcttttacga caaataaatt aacattctca gatcgcttga
      241 ttcttgcatt taactcttga acaggcgtag gcgatggaat ttctttttct tgcaactcta
      301 aaagcaccaa gtatccttcc aatacttctt gcgccatctc aaaagcatct tctaaagttt
      361 caccatgtgt atataagtct ggaaaatctg ggaatttaac tgcatattca tttcctttcc
      421 attcgaatac tgcacaaaaa aagtatttca tcgcattgca cctctctaat taaaaaataa
      481 tttttgcttt tttgtttcaa aaaaaagaat cgcctaccaa aaaaattgat aggcgatcac
      541 aagctaatta acctctaaag gagattaata ccttgctccc aaggcactat atatcttagc
      601 atacgtctga agcatcttca aacgagtaag atatattttg cattaaccta attttttttc
      661 tctataagtt ttttcatcaa actcactaga aatattaaac tggacagcaa aattgtaatt
      721 tctgcctttt ctaactttag caaaatcaat attcagcatt gatttttcat taatttcctc
      781 aactgattcc cttaaaacac gtctatcaaa attcgatatt gttttatact cattttctgt
      841 tttcgtaatc tcacgaattt taactatttc aatcagggga ttcttatata tttcagcttt
      901 ctcaaaatct cctttatttt tggcgataag atatttttta taatacatcg ccaaatattt
      961 gtataacacg atgctatatt ttttttgaaa agatgctaag tcttcaaaaa caaagaacgg
     1021 atacttcttt ttgattgaga caattaaggg aattacatct tctgaaaaac tgaaagatac
     1081 actatgctct tctaaatccc aatgaatccg atcaatagca gatacagtag tatacgtctt
     1141 atattttttg tctagattcg aaatttcata aactgcttgt ctttgcaaag tgttaactat
     1201 ggtttttagt tccgtttcaa gattttgatc aacatccatg taattaataa tcgctttaac
     1261 aggtaaatct actacgcctg ttttctttag ctttgtgggt gagatataac tgaacagcaa
     1321 ttgataaagt tcatacgact ttaaatccat tttcatgtca attgttgcta agaactcctc
     1381 attgtacttt gtcagttcat cttttttcaa aagtttttcc ttccttcacg cattttttca
     1441 gttgttaatc ataaaggttc acataaggca aataactcgt aattgatttt tcgagatatt
     1501 taacagtatt ttttattgtg taatcctcct tatgagtagc aacgtaagat aggtgattga
     1561 caactgattc aaatcctcta tctttggata aacgatcata aagcggataa actttattct
     1621 gaagtcggct cataagttta agatcgcata agtcggcaga actgatcaaa aagtttttga
     1681 ttagtaattt agtgtatttg ctattcatag ctttcaagta cgattgattc attttctctg
     1741 ttttatcttc tgcgcctttt aaataagcag ggtctctttg ttcttgctta taaggtaccg
     1801 gaactgacat ggtacgtgta agcaagaact ttatgtttgt ataagtatga cccttcttga
     1861 taagctcata gtcaatttta aaatcagtgt atttattcag ttgggcaatc ggttctttaa
     1921 ttacacgtct ttcaaagtct cgaaaagctt tatatttttt ctcaatacta aatttctttc
     1981 gtaaatctgc aatggagaaa acaggatttt ttaatctatc aagtgatttt gtactccatg
     2041 ttaagttatc tctatttgct tcatacagat taaaatcttt agtgaataat tgaaaaaaac
     2101 tgatcgcata cttgcttgtc aagttaacta cttgatcaaa tgtatattgc gtaaaatgat
     2161 tttctaagtt cgttaataaa ggcataattg aactactaaa tttaatagat actgcatcag
     2221 aaaaatcatt gaattcagta atttctagcg gtgagatgac tttgtattca gtcttaccgt
     2281 taggtttagt tctttgaatt ttaatgacag attgctgatg caatttttca ataacttgtt
     2341 ttaatcttga atgttttgaa gaagatgaac tagaagacat caacgcaaat aaagcctttt
     2401 tagatacttt gacggtatta ttaaaaggcg gatttttagg gtcaagatgt cctatagcgt
     2461 aaagataaat tttttgtggc atagcatcca tttcaacaat gctatctaca agttgattcg
     2521 ataaagtaac agttaaatca aactttttta gattgctttc ggtagtttca aatttcgggt
     2581 ttacattctt ttctgttatt ttcatacgat tgcctcttct ttctaagctt ggtacgagct
     2641 taaaaacttg gaactggtac gattccattg aataagtata gacctgtttt cgatacgtta
     2701 caataagaaa tgtatcgtaa atttataaca taactcaatg tcagtaaaac acattttatt
     2761 ttttccaatc aaatttcatt gaaaatacag cagaaatcgc tattttagaa cattttcaaa
     2821 ataaaaatcc ttttcaataa aatacgaaat tataaaaatt accaaaatta aaaaacaaat
     2881 atttttctag tttttagaaa taaattcaca tactttttct ttttttggat atattcggaa
     2941 taccacatcg ctttttgtcc acgtttgcat cgctttttgt ccacattcac atcgcttttt
     3001 gtccatgttc gcatcgcttt atgtccaccc taaaatctat ttaagattga tataaagcct
     3061 ttttggaggt tgctaaagtt ttaaagatta aaatatatat tataaatata aataaaaggg
     3121 gaagctgaag cttcctcctt ttcctttttt tcaattattc aaagttacac aagcaaaata
     3181 aactcctaga tagtgttagt tttttttaag attttttatt tttaagattt tgaaataaga
     3241 gaggagtaat cctcctttat taaatatctg ttaatatatc tgttaaatat ctgttaatat
     3301 atctgttagg ggaggcttaa tctcttggga gagtaagtat taaaggggta taaaggtaac
     3361 acttttggac agtaaaggta acaaaagtgg acagtaaggg taacagtttt ggacagcaaa
     3421 ggtaacaaaa gtggacattt gaggtatcac actaggataa gacgttacct tcggttttct
     3481 tgatttggaa ggcgaaaaaa tagaaaatta gattagtaac aaatctttgt tgactgttac
     3541 ctttagttgt gatttaattt aggtaacaag cagaaaaaat tgtttccttt aattagtacc
     3601 agattaacat gattaaagga gcgcaaaaag aagattggag agtaatcaat gtctcaagaa
     3661 gttaccgaaa agattattga tagtgatgta gatttagtat cagaacaaac aaatgcaatt
     3721 gtgcgttata acaatggtct gaatacaaca gctttgcgag gatttaagcc tgttgaaatg
     3781 gatgtgtttt gggctatttt atcaaaaatg aaacgtcaag gaacagcaga agtaactttt
     3841 gatttcaacc tctttcgtta tttggtcaaa tacgacagaa gagaaaagga cagtttttat
     3901 tctgtgttaa aagatttgag tgacaaacta ggtaatttaa cctaccgttt tgaagattca
     3961 gacgtctatg aacagctgtg gctttttcaa aaattccgga tagataaaca gaaggaaaca
     4021 gttacaatta aggcaaatga ggaatttgaa tttatcttaa actcaattgg caaaaacttt
     4081 actcgttttg aacttgataa ttttacaaaa ctaagaagta gctacacaaa agaattgtat
     4141 cgtcatctca tgcaatacag aaataaagag tggcgaaatg gttattgggt agtcaaagtt
     4201 gaagatttta gaagagcatt aagtatacct tcaagttata gaatgacaaa tattgataaa
     4261 agaatactag agcaggcgaa agaagaattt ttagctcctg atgaaaacgg aatacaggta
     4321 tttgaaactt ttgattatga aaaaatctat gcaaagaaag gaagaagagt tgatcggctc
     4381 agattcactt ttagtgagcc agaatcaaac ttgccaacca tttcaatgca taattggtta
     4441 gaagaagatt gagaagaagc aggttgtaat tttaatacaa cctgcttctt taaaaaagta
     4501 tgggtaacac ttttggacaa taaatgcaag aagttttttt gtaagcaagt ttcagtctaa
     4561 ataatatttg aaagaatggc taatctatac aaattttaac aactatgggt aacacttttg
     4621 gacaagtcaa gaaaaaaaac aatttttctt aatttgttga tcgactaaag gtaacacttt
     4681 tggacaacac agaataaaaa gtccggtgat atcagttttg gtaggaaaca tcttaatatc
     4741 tttgtgagat tgaaggtaac atttttggac aagtcaacta tttgttaaaa gttaatatag
     4801 agtaattaga taagtttttt cttttcgtct gataaatgga taatattagt tattttattg
     4861 ctaattagtc tttcatgatg actgaaatag ctaaaataaa tatctcgttg gataattgct
     4921 gatactttaa gggatatcta aatttgctga ttgatttatt tatgttatta tgatattata
     4981 ataacataaa tgtgttacgc ccgaacctca ttttaaaaaa atgaaggggt gagacaatga
     5041 atgctattaa ttggcatgaa tttgttaact atatggagag cttactagat gatctaaaaa
     5101 gttttagtat tgctgatgca gtaaagcatg ggttaccagt tagtacaacg atgaaattac
     5161 gaggattgaa aattccagat gaaattatta agctgagaac taaaacaatt attgaaaccg
     5221 caaaagtcgt tgacaatatg aaccatgaac taaaaggcaa gaaaacaact tacgagaaag
     5281 attttgaacg ttttttaaaa tctgttattg gaaaatatct aggtaaaaaa tctttcagtg
     5341 aattgagaga tgaagagatt gtacctactc caatggttag caaaatccga aaaaacaaag
     5401 ccgaggggaa ttactatctt ggtacaatag caaaaatcat tgattatttg gaaaataaag
     5461 agcaaaagaa aggaacgaag aaaggaagat aatttttgtg ttaaatatta atggggaaga
     5521 atataccgaa atgattcaaa atcccgaacc tagaattacc gaaaaaacgt ctgattgttt
     5581 gctcttttat gaaagggggc agacaaacta atggaaaaaa acgtcaattc aacatctacc
     5641 aaagatcaat acagtattat tggagggttg cttggtgata aggataagag aacacgtcac
     5701 ggattacgca ttatgcttgc aacttttcta ggagtgcttt ttttattaac aggcttgggt
     5761 agctacattt atcaattagg aaactataat tattatctcg tgaacaatac aacacctata
     5821 ggctcttcat tggagtttgc tcgatcagga gcagagatca ctatttcaga tgtgtggaca
     5881 gatcaaaaca gagatgtaac agtagtaaaa ctcggttact cgcaaaacgc tagaaagttg
     5941 ttatctacgc aaggaaaaaa ctacaactta tatatggcaa cgaaagaaaa agatcgtcct
     6001 aaaaagcttg aaatgagcta tgggatgtta agtactgatg gggatgcttt cttattccta
     6061 aaaggtaaat tagatgaaag agcttatcaa gtttttattg caaaccaagt atcactaatt
     6121 tctggtgatg atacagctga cgatgatacc aaagtcaatg tgaatgaagc agattcagtt
     6181 acaaaagcac tttctcaata ttctatgaat gatgttaata atgaaggagt aatttctttt
     6241 gggaaaaaag caaaacctgt taatactcct gataatatca atttcagaat aaatccatat
     6301 tcagattcaa ctaatgtcta tcaaggaagc tttcttacca aagatggcga tattgattat
     6361 tcaaaagttg tagcagttac aagtaagaat aatgttgtaa gtaagttaga agatcagtta
     6421 aatgcttcaa atactaacat caagaaaatt gaagctacaa aagctgagta tctggaacgt
     6481 ttacaacaaa atcctgacga taaagattcg caagaaagta ttcgtcaact agacagtcga
     6541 atggagaccg aaattaaagc aatcaatgat acacaaaaat tgattgatca atacaaaaat
     6601 gctgacttta acaaagattc ttttggagac atgaagacga catttaagta cctaactctg
     6661 actgactaga agtaaattat tcttatttta gaggaagtga acacaaacag atggaaaata
     6721 gtaccgagtt ttttaagaat attcaaactg aatttaatca tgctctcaca ccaatgaaac
     6781 caccgaaaca taagttagtg ggacgtgatg acgaactcca tgagttgtca ctgatcttga
     6841 atagaccgga aacaccgcaa gctatcttgt taggtgaagc cggaacaggt aaaacagcac
     6901 ttgcaagtga atgggctgaa agacagcgac aagatggaaa gaaagtacaa gtatacaaat
     6961 tatcaatcgg acaattgcgt gttgggggag atgatgtcct ccaaacacgt ttagaaacat
     7021 taattccaag attgaaagaa tatgagcgaa ttttaaaatc tgaggacaaa gaaaatgaat
     7081 ttgtcctctt cattgacgag tttcataaag ttgttagctc ttttggtagt ggcaataaaa
     7141 ttgggggtga ttcgctaaaa gaaccgttag caaatgaatc acttcaattg attaccgcta
     7201 ctacaagaaa agaatatgaa aactatatca aaagagatga tgccttaggg agacggttac
     7261 aaccgttaga attgaatgaa ctaaaaagcg atgttgtcgt cgatattttg agacaatggc
     7321 taaatacatc ctctgttaaa gatgatcctc aggcttcttt taaattatcg caaaggattc
     7381 ctgatgaatt acttcaagaa atcgtacaag caaacaagct ttatcgtgag gatttgtccg
     7441 aaccggctaa atcaattgcc gtaatggagg atttgattgc aaaagatcga gtagacggaa
     7501 atgcttataa tcgagaaagt ctattgcaaa tatttggtaa gtataatatt cagctagatt
     7561 ctgattttga tatggaaaaa cttcaagagg aattaaagtc cataaaaggg caaccgttag
     7621 cagtttatac tataacaatg ttgtttaaac gtatgaagct tggatttgat cgttttaaag
     7681 gtcgaaagca accaaaagca actgctctgt ttacaggatc aacaggggtc ggtaaaactg
     7741 caatggctaa agccgtggca agaggacttt atggcgatga aaataaagtt ctcttagtta
     7801 atatgccgga ctacaaaaca aaagatgatg caatgcgttt tagacgactc ataggtactc
     7861 atgtaaggca tgatccttct gcgattattg tgtgtgatga atatgagaaa gctggtgtag
     7921 aaacactcga tgccttttta tcaattttag atgaaggtag gataagtttt actgaaagag
     7981 gatgggatga tcgagaagtt cacgacactg tttcattgtc aaatacaatt ttatttgcga
     8041 caacaaatgc aggtgcggag atgttcgatt cgttaaacag atatagctca ctatctcgtg
     8101 ttacaaacaa ggaaaattct aatgaatata gcactgaact aaaaagagaa taccgaaaag
     8161 tcgcatcagg gatcaaacaa gccttgattg gtgaaggtct aaagcctgaa gttattaacc
     8221 gatttggacg aatagttcca ttttgggctt tatccgaaca aactcttctg gaaattgctt
     8281 ataatgaaat gttgaagagt gctgaattag tacgaaaaca tttaaatata gatttacgtc
     8341 ttccacctag gaaagaaaga gtatatggtc aaaattttta tgcagacgat ctctccatgc
     8401 acttagtttt tgacatggct tcacaagagg attctaaatc agggggtgca aggaatatct
     8461 tgcatttagt tgaatctgag tttgaagaag cgattactga agcaataatt acttatccaa
     8521 acgcaaaaat attcgatgtt aagttgagtg aagccggagc gtttgaagga aaaggtgatt
     8581 ctaatgctga aattattgca aagccttccg ttgctgaaag taaagaaaac aagattgtat
     8641 tttcgattgg aggggcgtag ctatgaaatt caagaaatat ttactacaaa accttagaat
     8701 aaaacgatgg gtcgtaagag gtctagttat tggaggagta ttagcagtta ctttaggcgc
     8761 tccttttgga atgaatgatc caactcctga caatcaagat agcaaacaag aaacagttat
     8821 aaataagaac gttgcatatg cggaaagtaa accggcagaa caaggaatgt ggacactata
     8881 tgctcgtatc ctaatgggag ataaagaagt taatgatagt gccgctaaaa ctggacaaat
     8941 gaatttgtta ggacaaggtg gtatcagtgc cgatttccct tattctgaca tttctaaaca
     9001 aggtaattcg attgcagggg gtaatgcagg tactcaacta gcatctactt tagcaactta
     9061 tagttactac ggatatattg aaacagtctc aggaaatgct attgccagtc aagcatcatc
     9121 cattctagga agtattggta ggacgattgg tagttttatc ggatcaatta gtttaatcct
     9181 ttctaatatt gctgaatcca taaatgaact ccttggaaaa tttttcatga gtatgaatat
     9241 attttctctt atggggtggg gagaaggtgg atcggatgta tctaatccgg tatctaaagc
     9301 attaactagc ttgcttaaca atttgggatt aaataatgaa ttatttaaag cgatagcaag
     9361 cttatctatg ctctttttca cagcaatact aggttatcaa ggaatgaaat tattgtcgaa
     9421 agatggtgtt aactcgaagg gattatcacc agtacttgaa aaatggattg ttcgtatttt
     9481 tgtattgttt atattcttac cagtggcagg atcgctattg ggagtttata tcaacggcat
     9541 ccagtcagca aaaaaagatg gagatatacc aacaaatgtt gtttctgact acatcattaa
     9601 cacaagactt tgggcttcta ctcaaaatct tgctccatca tctgatactg gtggtgatgt
     9661 acctgacgga aagcctacat ccgaaatcaa gttcgttgat acagattatg aaccatctaa
     9721 aaaatctgca atgataagag cgatcaatac tagcagttat gccagacaaa ataatgatct
     9781 agattctaat tcagtcggta tggctctatt aaaagaatgg tcatcgaatg atactttcaa
     9841 tattaatact tatgccggtg atgttaatgt cgccgaaaat gatgcttata tgggaagagc
     9901 gcctgctgtt tggacaggct ataatttagg ggatggaaaa gaaggaagtg caattaagaa
     9961 accaaccaat ttaaatggat atatttggtc agcaaatcaa aatgtgacag acgacaatcg
    10021 caagcctgat ggaaaaggaa cagcgcataa ctttgatgct actcaacctt ggggcgttgc
    10081 taacaatgca acttttagca cacagtcggt agctttattg ctccaatcgg cacttgatac
    10141 atcgggagct aagttttatg cgtataatat cgctccttca ggaattcaag gcagtgctaa
    10201 gaatatgtca acagtaaaaa cagaatggcg tacagcatcc ttagttggtc atggagctat
    10261 gggtaagttt ggctcgtggc tagcgatgaa cgctgaatca atcgttttag cagttcttta
    10321 cttagcatgt gcttgggcga tgttcaatgt gaatatcgtg aaagcattta aacgtgaatt
    10381 tattcggatt tgggaagtag ttctaacagg atcacttgca tcaggagcag gtgtgatggc
    10441 aatttcatta ggtgttgtta caacatctct gatcggacta caattaccta tctttctgat
    10501 tacttttatt cgtgcatttg gtgatactgc tgtgggattc attcctaata cattagttgg
    10561 cgatggcgta gcaaatattg ttcgatctgc tatgatttta ggtgtaggat atcttttagt
    10621 ctttaaaaaa ggcgaaaaag taaataattt gattaatatg cttattggct taccacttga
    10681 agtagcgttt gggtttgatg aacgtgctaa gttattattt ggtaatagaa atgatgctgt
    10741 gcgagttgtg gacggcgttt ctaaagcaat caagaaaagc caaggagcta aaaatcccta
    10801 ttatgatggt ggcaacagct cagtaaaaga tatggttgca ggaggtcttg gagcagttgg
    10861 agcgcaaaaa atggctgaac gtttgaacaa tatgggagat agcaatgctg atgctaagtc
    10921 cattgctgat aaagtaattc ctgaaacacc agaaggaatg tcattcaacg gtggcggagt
    10981 tggttccaca atcaataata cctctttcaa agatttagtt cgtcaagatc aactacgtaa
    11041 taactcccaa caatatattg atcatttagg acgtatggtt gatgaaaacg gaaactttgt
    11101 cgatgaaaat ggtgaaatat taaaagatag taaatacggt aaagatgcta aaggcaactt
    11161 tattgacgaa aaagggcgtt tggtcgatga agatggaaac cttatagata ctaatggtaa
    11221 accattggaa gatagtgaag atggtaatat tggtatggat agcgaaggac gtttaattga
    11281 tgaaaaaggt cgcttagtcg atgaagatgg aaatcttgtt gacgaaaatg gacgcaggtt
    11341 gctaaatggt gattttggtc atgatagtaa cggacgttta gttgacgaaa aaggtcgttt
    11401 agttgatgaa gatggaaatc ttgttgatga gtttggtaat aaacttggta aacacagcga
    11461 tggatataat gtgaatggag atttagtcga tgaggatggg aatatcattg agacgagaga
    11521 tgcgcttgtg gcagatcgaa atagagaact cgaacaacaa cggcatacgc caattgctgt
    11581 tgcaattcct gagaatgaag aagacgagat tactttagat caaactcatc aagaactgag
    11641 gaatgaaatg agtggtgagg acgtgtcaga cagagtgcat cgggaattag gagatggagc
    11701 atctggtgaa agtcagtcaa gagagcttaa cagcaacttt gataatagtg ctattcggaa
    11761 tgacacagta cttaaagaca gagaatcgct aacaaaagaa acggataaag aacaatcaga
    11821 tgtgaagaaa aagttcattc ctactcctaa tgttaatcaa acagataaat atgaaacaaa
    11881 tattggatca ggaatgtcga aagacaatca atcaattaga gaacctgaac tgacaaataa
    11941 aaataataga acaaataatt ctaaatctac ttcagataat tcagggaaat ctaatcaatc
    12001 aaccaagaaa acatcaacag ctaaatcagc cgttgaatat ctatcaaaaa ctggtaaaaa
    12061 tgtttacacc tcagcagata aagctttagc aaaagctcca aaaggtagtc tagcagggat
    12121 aacctatgaa gctaaaaacg aagctattaa aataggtaaa ggaacggcta aggttgccaa
    12181 ggctactgct acaggagcat ccaaaactgg taaagtagtg aagaaatcta ccatgtcagt
    12241 caaggactat attgtaaaat acggtaatca aaaatcaact gaaaataagt ctgaagacga
    12301 gtaaaaagaa ggagcattga tttgctcctt ctttttactt tgatttctaa ctgaagatgt
    12361 caaggaaaat ttctatttat gcaaaaaaat tattttaaat tgtctataaa tgaattaaat
    12421 atttactgtt caaagttgta taattaaaaa gggcttatgc tggtaaagaa cagaattatt
    12481 atctaaacaa aaacttgata aaccagcatt tttagccaat aaaaaaagtg aaataattta
    12541 atggaaaatc tcttttaatc atcgaaaatt tatgttatta tgataacata aagatgtaaa
    12601 tatcgagacg tctttttctt agttagatca ttgacaagtg aacaaataag atttgtcgat
    12661 gatataatta acttattaaa aatattaaaa aggaggtttt tatatgttcg attctattaa
    12721 aaatttggtt agcggaatga cgcctgatat gaaaacttta gtacaagttg ttttgtattt
    12781 cttggcattc gttttttcgt tgaaaccagg attcactgca atgaaatttt ttggtgatgc
    12841 aaaatgggga gatggattaa aatatgttgg ggcgattatt gcaatcttta ttattcctgt
    12901 aattacaacc gttatgcttt acacaatggg tactaaaaca ggtaatgatt tgaaccaaaa
    12961 agctaatatc atatcagcac ttattccagt tgttggtatc tatctagttt caaaatttcc
    13021 aaaaaatgaa ctgttaggtt aaaagtgaat tgaacaaatt tggataatat gccgatttgt
    13081 tcaatttact ttttaccaaa aaactacgct ccaagcgtag tttagaagtg agggaagaaa
    13141 gtaaatgtaa gatggttttt gatagagaaa aaatgttagc tcacgccaac gaagtattaa
    13201 tgagcagtct gaaaggtacc gaactggcaa agataatgaa tatgaatgtt aaccagttct
    13261 atgattatcg aaatgggagt aaaaagattg aaaaagccag acttgaaacg cttattaaat
    13321 tcgaaaaagc atatgtatat atgctagata aacagaaaag aactattgac tgaaaaaagg
    13381 agttttacaa taatggcaaa attcaaagat attaccgaat ctattaatta tgtggcagaa
    13441 acagttcctg tagttgcact ctctttgaag ccacaattac tcgaagtgta caaaaaagaa
    13501 gaaaaagaag cgaaggcaaa aaaagctaaa gcaaaggtat ggaaagaatt cgatgcgttg
    13561 ctgaccaaag atttaccaaa gaaaaattat atggtaattg aagatgtaca aaagctcaac
    13621 agtgagggag ttatagatga ggatgatgct attctcgatt ctgagaagta tgtcgttcta
    13681 ttgaagtcaa gtttgaatta tgtagctgat ctaccagaaa aatttaaaga gaagctatta
    13741 gcaggtgtcc gttttatgga tagtagtggt caattattag ttattccgga tgatcctaat
    13801 gtgtttgaat cagaagttga agaagctaca gacttctata gcgcagtaga gcaattagaa
    13861 gtagctacta atggattaga acgaattgct gagaccttca actcttctga agaagttgaa
    13921 tttgaagccg tagcagaaaa tgtaccagat gaaaaagaag ctctttttgt ggatgataac
    13981 aaaccaaccg aagcagaaaa ccaagaagta agcatagaag aaacacaaat acaaacagaa
    14041 acctaccaac aaccaaaaat tgatgaaaaa cccgaatccc aaagtgacaa agaaaattat
    14101 gtttatgaag ctgaaaaaat cacatctgaa agtgatgtac cgaaaaactt agatgctgat
    14161 tttttagaaa aaaattttga acagatcatt gctgacaaat ttcaacctgt agttttagat
    14221 gagattaatg accctgtcga tttaaacgag gagatgctat ccatacctga gttgaaacca
    14281 ttgctgtttt tagctaaaga gagtatgaac gctcgcatct ctattgctaa taataaaatc
    14341 aacagtgaat taaataagac gaaggagcaa ttgatcctca aaggaaagca aatgttagct
    14401 gatgagcttt tgcggataga ggaagcaacc tcattaaatg gtcaaaatca gtttacaaat
    14461 acactatcgg ttgctaaaga gagctacgaa gcagaagcat ctaaaattga tgatttagtg
    14521 atggaaaaat ccaatatcat tcgtaaagat tatgatatgg cgaaacaggc ttatttggat
    14581 gctgaactaa agaaactaga gcaacggtat gatgctgagc atttaccaga agttaaacaa
    14641 caagaagatg aatatagaaa agcaactgaa gaaagtcttg atcaacagta ccaagaagca
    14701 attgaatcta ttaaaaacga tgcattgaaa caaagccaag aagcaaaaga agttgtggtg
    14761 gaacgaatta tcaattcatt gaaaccagaa attgaaaaat cattaacaga cgtgcaagaa
    14821 aaaactttag agattgtcga aaaaacaaag tcagaaaatg aagctgagtt ccatgataca
    14881 aaagagaaaa caacagatgt ggttgtcaaa tattttgcga acaaagaaga agttgaaaag
    14941 caggtagcta acaacacgaa agaattagaa acaaaacttg tagagcaaca aaaaactatc
    15001 gaagaatata aagatcgaat gaaagaggtc aaagatttag cagagggtta taaattaaat
    15061 agcgaaaagt tggcagaaca aagtgaagct ttaagagaag acttatctcg ttcccgaagt
    15121 gatctagctc aaatggcaga aaaaacgaaa tcattagcag ttgctacaga aaatcaatca
    15181 agttcaaaac gtgattttac aagtaaaaag atggctacta tcatttctgt cgctgcaatg
    15241 gcttgcggaa cgatcttggg atcgtcctac tttattagtc ataataatca acaagcacag
    15301 gctcaaactc aaacatctat ttcgagagtg gaaaattcga ttgggacaaa tacgaactct
    15361 gcttcaacaa atagtagcta tgaagcaaca ccaactacag agttcaaagt tgggaaagat
    15421 gttgtcatca gtattgatgg gaaaacaact aaggctgaag tgatttctat tgatggagaa
    15481 aaagcagttg tgaaaactcc agacggaaat cagtacaaaa ttcaagctca cttatctgat
    15541 aataactaag gagggctatt atgcgagaag aaaaaatagt ttatccagta attcaaaaca
    15601 atcaagaaaa catgacctct tctcagcact tacaggaagc acagctcgtg tatcaggtaa
    15661 atgaactacc acaaacgaac gagacaatga gcaagtcaat atctttaatt ggcttgctta
    15721 ttgttgcaat agtgataact gtaggtgttt accgtaagtt gaacgtgaaa ggaagtattt
    15781 ctgatggcag ataactcaaa ctatggattt aaacataact tacatcaaaa accttctgaa
    15841 acaattgtca gagttgaatg gcttgaaaag catgtcaata gcacctattc tgttacagca
    15901 aaaagattaa ttgtatcagc tatagttgtt gcagtcgttg ttcttggatc aattgtttac
    15961 ttagtttctg atacgattaa actgaaattt atttttgttc caacgttatt aatgataatt
    16021 ggatttctta ttctaattag ccagtatatg tttattgatc catctagtgg ttcattgatt
    16081 tctggttcta ttagactgtt caaatcagta gttaattcag caagagtatc tatggggaaa
    16141 aagaaaccat ttcgaggcac agagatttta aaagtgaaac aagatggaac aatcgtatat
    16201 cgagatggga gtgtagcatt aatgttcaat ctagatgggg ctacatcact aaccgctttt
    16261 ccagaagaag ttatggctca ggaatcgaaa tcaatacaat atcaaaatac cagagatcgt
    16321 tccttagctg aaatcaaaat cacttcatct caacctcaga atacggaaag acaaactcga
    16381 aatatgaaaa tacaaaaacg aaatgcaagg agtaacaaag cagtctttga cataatagat
    16441 cagcaagaac gatttgtatc agaatatatt gatggtgtga aaacaacagt aatccaatat
    16501 cttatcttga aatctcaatc taagatggta ttggatgaag gtgtggaacg tttagaaaac
    16561 tatacgagta gaggacttta ttatagtgtc actcagttgt cagctaaaca aacaaaagaa
    16621 atattatctg atatttatgc actgaaatag aggtgagaga ttaaatggaa attatgggta
    16681 gatcacaact attaaatttc aaaaaaaaga gaaaaactaa aaaagaacaa aaaactagaa
    16741 attcaaaggt agtgaataat acctttgtta gtgatttaac atctttagtt ggttatcaat
    16801 ttacaccatc ttacattagg acaggtaagc ggtggggaac aattgtgaaa ttagtgaata
    16861 cttatggaat gaatagaaat tatggatttg gttggatggt taaagctatt cctgaagtga
    16921 gagtagaagg ggtaaaagcc tatctaattg aagcagataa gccagtgtca caaaaagatc
    16981 aacaagaaat aatgcacgag aatgttgggg gatcaacgaa tagttatgaa aataatttag
    17041 aaggaaaaaa ttcagatgct gaaattagca aaactgttat gcggattatg gatttgaata
    17101 cagcatcttt actagacagc atggcaaata aaattattga ttggaagcta gaaatcctat
    17161 tagtatccga ttctcctaat aaaatcagcg aacagctaat taaactccaa cggttgtatg
    17221 atgaaaaaat gtatggatta aagcttatgt catatggagg agatcaacgt gatctgtttg
    17281 atagactttt aagtgaacca atcgggaatg tctatgagta tacgtctatg agttcggatt
    17341 ttgcaggaaa tgatcatgca attcgcaaag gtttagatga tgcgaatgga tgggctatcg
    17401 gcactatgtc tactagctac gcaagtggtg tagcttttat ggcactggat gattctttta
    17461 caagtaaagt tttagtggca tcacacaaaa acgctacaat taaaggctat gatgaaaaaa
    17521 tctcagcatc atcactttgg gggcaattga ttgctaataa tgcaatggca aatcataaac
    17581 gaacttttca tattgtctta aataaatttg attactttgg tgatccaact ggtagaacat
    17641 ttgttgccaa accttcgtta aacagtgtaa tagaaaaggt taatttggct gaagaaggtc
    17701 ttaactcttt ggagcttttt ggtaatcaaa aggacattgg tgatttatat agacggaatc
    17761 tcgataaact aactcagatt ttttatttgt tagcaggcag aacattagat tctgcaacta
    17821 gagttttatt gaaaaaagaa ttaggtaact tttatataag tcagaaactt tgggatatta
    17881 attatgaaga acgttcaaaa cttttggtga acagtagtga tacaatgcca gttcttgggg
    17941 attttattag ctatttgact aatgctgaaa caaaagccaa aaaatctggt actgaaaaag
    18001 aagtcgatcg tattattgta ttaaaagaca cattgagaac agcattagac agcttctcta
    18061 ggatgtttaa tcaaagaacg acactgaaag aaatcggtaa aagtgacaaa ttacagtttt
    18121 actataactt aggtgatcta agagcagaaa ccaatgttat ggaagctcaa tttttaaata
    18181 cctttgattt catcacatca caagcaaaaa aagatgatat tgttatgatt cacggcttcg
    18241 ataagttatc tattgaaacc gttgggctaa ttaaaaaaag attaacaatg cttgaagaag
    18301 atggaattcg tcttgccttt ctatttgata caatcggttc aggagaagac aatgaataca
    18361 aagaagatat tgaaacagct gatatattca atacagatgg aaaaatttac caaagttttg
    18421 aaagtgattt taattacact atcattggaa agatgaccag tggagagtta atgaagtatg
    18481 aaaaaaaagt tgctcctgct agaaaaggag aagatgatca aaaactttct gttgatttga
    18541 gacagtattt gacaacagga agaagtaata aatttcagat caggagacca gtggatctga
    18601 ctaataattt tgttattgga gagtttattc tttaagtttt atctttgatt ttgagtgtat
    18661 aaggaaagga taagaaaaaa catgtatatt tgagactaaa aacagtctta gagaacatgg
    18721 tacgttttgt gaaaagagaa ctatagaaat ttcataaagt gaaaccttac tgagtgaaat
    18781 aattatagtg gaggttaaat aatgaaacca aacataaaaa ttttagatcg aatatttcta
    18841 ggtagagaca cagaagtaat cctaattcaa catgaagaag gatttgaagt tagtataggt
    18901 attcagaaac ttcaaaaacc tcattattgt aatcagttgt ataaaaactt tactgatgaa
    18961 gaaaaagcga gagtttt
//
LOCUS       DAPBBG010000052        18887 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPBBG010000052 DAPBBG010000000
VERSION     DAPBBG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.053"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..355)
                     /locus_tag="RD477_002150"
     CDS             complement(<1..355)
                     /locus_tag="RD477_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDT7634481.1"
                     /translation="MLTKKELIDHIDETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIIDILDLKVDPELGGILRGKEGYFPAYHMNKEHWISVDLRKI
                     EKLDEPDCRQKLSVRT"
     gene            499..1938
                     /locus_tag="RD477_002151"
                     /pseudo
     CDS             499..1938
                     /locus_tag="RD477_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1945..4497)
                     /locus_tag="RD477_002152"
     CDS             complement(1945..4497)
                     /locus_tag="RD477_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDT7634482.1"
                     /translation="MNWYKLTVEDTLKNYQTSTEGLSQEERQKRLTDQGYNEIEARQQ
                     TKRWKKIVKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADVRLIEVHDLLVE
                     ESILTGESEASEKNHTVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFSYGMDWPILLSAMIAL
                     VVSMVPEGLPAVLTMILSVGVNEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEETRVLSNMKNCQELKTKEEQKTENLSGNPTELALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHTEGKGAVIYVKGAPEVLLAKSTLSSSEQEAWGQTATAFAKK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSEEELIRQVPKVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILILALILNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVALLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKSLFLSLGILALLQGMVIFLPS
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREFSR"
     gene            complement(4610..5941)
                     /locus_tag="RD477_002153"
     CDS             complement(4610..5941)
                     /locus_tag="RD477_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7634483.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIALAGPVVI
                     LVYLICGVAIYGLMRAMGDLFLANSKFNSISDFIDYYLGEKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIV
                     FFILLGIGLSVSYFHTQSIGIKANFGQLNELLPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYVGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQSGAAIVNLMILVSSLSSANSAVYSTSRILYQTAKEKSLPPWFCALSKKGVPLHGIL
                     ITGTLFLAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHIQFVKEKKRNTIP
                     KYKDLALLVFFVAIAFSLLFERMTRFIPFLLVIWFVLLSFVYKRISDRNIN"
     gene            complement(5907..7385)
                     /locus_tag="RD477_002154"
     CDS             complement(5907..7385)
                     /locus_tag="RD477_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7634484.1"
                     /translation="MKDAGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKNWGFIVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLTFDMAKIGGKIGVWLGLY
                     VPLTMMLLLGLAAWIKVGIVPTGTLGNFSWDKVIPDSTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGISVLRLRY
                     THAKLNRPFRIGKKENKSNLGVWAIVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7692..9527)
                     /gene="tdc"
                     /locus_tag="RD477_002155"
     CDS             complement(7692..9527)
                     /gene="tdc"
                     /locus_tag="RD477_002155"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDT7634485.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMADGWGHITADGSIANLEGLW
                     YARCIKSIPLAVKEILPEKVKNMSEWALLNLSVEEILEMTESFTDEEMDKVKAASSRR
                     GKNIQKLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     DQKIPILGVVAVVGTTEEGQVDSVDKIIQLREKLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHYVTIDKEVYEGFRAISEADSITIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVEAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLEQLHFTVKGKTIEVYPLNHPDFNMVNWVFKVQDC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFIERMGLSKEE
                     WQKEQQITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIK"
     gene            9997..11424
                     /locus_tag="RD477_002156"
     CDS             9997..11424
                     /locus_tag="RD477_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634486.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIVYTHREKDCYTLYFSEHIPRCKIMQQLTQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFHDCGIEIDRYSPRFTEMERRLLLLNVAYRL
                     GSFKSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQLGTVTID
                     PTFIEEIKKRPIYNYVESTWETTDFQTYYKKEEFVFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFETHFRQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQANLYKDVQTLFSSWAKRLPYDLRFNPNCMRAFVIELSGILRLTKERLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYASISDIKKYAQQPSNRVLCERTLYTPDA
                     VQYENIIPISINTIERAIISAVQNK"
     gene            complement(11585..11950)
                     /locus_tag="RD477_002157"
     CDS             complement(11585..11950)
                     /locus_tag="RD477_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDT7634487.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLIWGVCLSIKDFLKYLF
                     VHTEAKNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVPNK"
     gene            complement(11947..12312)
                     /locus_tag="RD477_002158"
     CDS             complement(11947..12312)
                     /locus_tag="RD477_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDT7634488.1"
                     /translation="MFHSNLIEFLEWIIAILNICSIGILVAGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEIG"
     gene            12753..12881
                     /locus_tag="RD477_002159"
     CDS             12753..12881
                     /locus_tag="RD477_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDT7634489.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            12938..14632
                     /locus_tag="RD477_002160"
     CDS             12938..14632
                     /locus_tag="RD477_002160"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDT7634490.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKDTPMQDEITVTGLKMTNTLTQ
                     EEEHVTVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(15036..15896)
                     /locus_tag="RD477_002161"
     CDS             complement(15036..15896)
                     /locus_tag="RD477_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634491.1"
                     /translation="MKQIQNKWNKRIIVMISSVCLFCLTVMLCLISTLANQHFMLEQN
                     MQSDYAKKVAAEINREIQSYHYEIGAPAEALEKIVSEELVQKNIELFIRNRYEKEPVE
                     LIGEKEVEEKLQEIINTYKQEEAEDDPFASEENNLLIYMTSGIFRRGIQSIYLMSFID
                     QILTIRERMVSFLPMISSILLVLLSGVFYLYRMSWKKVLWSFAVVIGGAGFQLLSLSV
                     SLYLYDMASQLDIRTQALEHLLDTYILSFIQFFSVVAIVEVVIAAGLWWIGKRVNTEK
                     KVFSKRNGYK"
     gene            16187..17320
                     /locus_tag="RD477_002162"
     CDS             16187..17320
                     /locus_tag="RD477_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDT7634492.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPRILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDETNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNNLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLEEAAQAYEIFDKKEDKNIKVVLKP"
     gene            17351..17665
                     /locus_tag="RD477_002163"
     CDS             17351..17665
                     /locus_tag="RD477_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDT7634493.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAVFLTIRPILRIVRNIFIYDEKRTRKKHGK
                     AV"
     gene            complement(17802..18260)
                     /locus_tag="RD477_002164"
     CDS             complement(17802..18260)
                     /locus_tag="RD477_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7634494.1"
                     /translation="MERRTVIKLRDRPTLIKETAEWFASKWHLPVAVYEESIQASTRK
                     NTSVPQWYVVLNEWGEIIAGAGVVDNDFHDRVDLTPNVCALFVEKDYRNQRIAKMILE
                     TIKQDFAEMGVKVLYLVTDHTDFYEKYNWQFIGMAQDNEGIPVRVYQMTL"
     gene            complement(18482..>18887)
                     /locus_tag="RD477_002165"
     CDS             complement(18482..>18887)
                     /locus_tag="RD477_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634495.1"
                     /translation="ATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPI
                     VLNPIMFIPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPVIGGVLATKSIAGGV
                     LAAVNLILSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         5869 a         3874 c         3261 g         5883 t
ORIGIN      
        1 ctgtcctcac agacagcttt tgtctacagt ctggttcatc tagtttttct attttacgta
       61 gatcaacaga gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc
      121 ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aaggatgtcg atgatcctat
      181 ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt
      241 ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg
      301 gagacaaatt gtatgtttca tcgatgtgat cgattagttc ttttttcgtt agcatctgct
      361 tccatctctt tcttattgat tcactttatt caaagatacc acgtaagaat agaaaaatga
      421 atactggttg aaagatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa
      481 tagaagggag tctgatttat gtattcacta atgaaaaaaa tcattaccga aaaagatatt
      541 cgacgtcatg tttctttagt cgaacaattg ctgaatcata caaagataac ggtaaatgaa
      601 ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc
      661 tctcagctgc ccgaaggctg ggacatcctc agcgatcaag caggaataag tttgcaaaac
      721 catcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtt
      781 gagctgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagcggat
      841 cacgggcttt cttatgggac attaaaaaga catgtgacta aaatcaatca gcgtttagca
      901 agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt
      961 gcgattcgta ctttctatca tcgattgctg attcctttca ctcataacaa ctattttttc
     1021 gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact
     1081 gaactagctg ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc
     1141 cgtataaaag cgaattgccg attagattct tctatccata tcaacagcac actctcctcc
     1201 ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact
     1261 gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga
     1321 caagaaatcg ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta
     1381 gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac
     1441 ttggctctcc tcgtattaaa atatcatgaa tcgccgatac tgatttaacc tttggaacgc
     1501 caataccatc gttttttaga gacgtatgat cagcaatatg cacacttgta tcagcagaaa
     1561 gagaatttac tgaacgatct aaaaaaagaa atggaaatca atgaaccaaa ttactttctt
     1621 caactcttgt ctttactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat
     1681 gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa
     1741 gattacttgg gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt
     1801 tcttttgaaa aaacagatat cttgatttcc aatttccctc tggattctct agatatccct
     1861 attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact
     1921 ttcaaaactt ttttatagta acggctagcg actgaattct ctagctgttt tttctttttt
     1981 gcgaaacagt ttattcatga tccctttttc tatttctaca actaagaata gaagaatcac
     2041 atttgcccca atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg
     2101 gccgcttggc agaaaaataa ccatcccctg caataaagca aggataccca atgaaagaaa
     2161 aagcgatttg ttttgcaaaa gaccttggtt caacgacggc ttggacaact ctctgcaatt
     2221 gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct
     2281 ttgcccttca aattgcatag ccaataaata ggcaggaacc atgatcagta gtgcgacata
     2341 gatgatccga aacaagtgat aacctgatag gattccttgc tttggatctc ttggtggacg
     2401 cttcatgatt tctggatctg gcgactcgaa ccctaatgca taagataaag tgattgtcgt
     2461 taccatattg acccataaaa tctgtactgg ggacaaaggt agcggccgat tcagtatcag
     2521 cgccaaaatc aagatcaatc cttgagctaa cgcagttggt aagaagaaat tgatggtctt
     2581 ttgcaagtta tccaaaatgc gccgaccttc ttttacggct tttgcgatcg tatggaaatt
     2641 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
     2701 agcgattcct atatcggctc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
     2761 tccgacgatt tctccatttt tctgcaaagc tttcacgatt cgcagtttgt gttctggtgt
     2821 cgttcgagca aagacatcga cttttggtac ttgtcgaatc aactcttctt cagacataag
     2881 atccaaatcg atcccttcta aaactttagc agtatgtttt aagccaattt gttccgcaat
     2941 cgcttttgct gtgtctttat gatcccccgt aatcattttc actgagatcc ctgcttgttg
     3001 gcattccttt acagccttga ctgcactttc ttttggcgga tcgatcaacc cggcaatccc
     3061 tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt
     3121 gtaagcaaat cctaaaacac gctgaccttt tttcgcaaac gctgtggctg tttgacccca
     3181 tgcttcttgt tctgaagaag aaagagttga tttagccaat aatacttctg gcgcaccttt
     3241 gacgtaaatc accgctcctt ttccctccgt atgcatcgtt gccatatatt tgtattcaga
     3301 gctgaacgga atctttgctt ctattttttt caaaggcaga ttggctgttt cagtgtgtcg
     3361 aatcagtgca agttctgtcg gattgccact taaattttct gttttctgtt cttctttcgt
     3421 ctttaactcc tgacaatttt tcatattaga cagtacgcga gtttcttcct ttgctgctac
     3481 atccacaact gtcatttcat tttttgtcaa tgtccctgtc ttatctgaac agatcactgt
     3541 catagaacct aatgtttcta cagaaggcag tcctttgatg attgcctttt cttttgccat
     3601 ttcatttact ccgacagata ggatcatggt caaaacagct ggcaatcctt cgggtaccat
     3661 tgacaccacc aaagcgatca tagctgaaag aaggataggc caatccatgc catagctgaa
     3721 tgttgtaaaa acaactaaga acaagatcaa acaaagaatg ccttgaaaaa tttgtttgtt
     3781 caatcggttg atcttcttga tcagaggcgt cgtttgttgt tcgaccgatt gcagtgcttg
     3841 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
     3901 agattgtaca agtgttcctg aaaaagccat atttttctgg tcaccgattg attcttctgc
     3961 tgccaaaaca gtatgatttt tctcagatgc ttctgattct cccgtcaaaa tcgattcttc
     4021 cactagtaga tcatgtactt cgatcaaccg aacatctgct ggaacaacgt ctccagcacg
     4081 taagacaatg acatctcctt ttactagttc tttcgcagga attttttctt gcatcccgtt
     4141 cgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaacg attcttctgc
     4201 ttttctttct tgccaataac taacgagtcc gttgacgatc actacgaata aaataatact
     4261 tccttcgatg tattcgctcg aagcaaattt caaaaaagag gcaatgatca acacgatcat
     4321 cagcagatca gtaaagtgct ttactatttt tttccatcgt tttgtctgct gtcttgcttc
     4381 tatctcatta tatccttgat cagtcaggcg tttctgccgt tcttcttgcg acaagccttc
     4441 tgttgatgtt tgatagtttt tcaatgtatc ttctaccgtc aatttatacc agttcatcct
     4501 atcccctcct acttaattga tacttttatt atacgtaggg agatctttca ggcgtatcga
     4561 ttatttcaga gaagtctgaa ataattcgtt ttgccggata tatttagtct taattgatat
     4621 ttctatcgct tattctttta tatacaaaac ttaagagaac aaaccagata actaataaaa
     4681 aaggaataaa acgtgtcatt ctttcgaata acaagctgaa agcaatggcc acaaaaaata
     4741 ccaacaaagc aaggtcttta tattttggta tagtgttccg tttcttttct ttaacaaatt
     4801 gaatatgtgc acataagatc gaagaccaca caaatagaaa acaactcgtt gtcatacctg
     4861 ccaaaagctg aaaaaacaat cgactatcag cgacaaaaaa atgtagacaa agacctgcaa
     4921 gaaataacgt tcctgtaatc aaaataccgt gtaaaggtac gcctttctta gaaagcgcac
     4981 aaaaccaagg gggcagactt ttttcttttg ctgtttgata taagatcctg ctagtactgt
     5041 aaaccgcact atttgccgaa gacaaagaag aaaccagaat catcagattg acgattgctg
     5101 ccccgctttg gcaatggatc atttgcaaca tagtgactaa aggactttct tttaaatcta
     5161 aacaatgcca tggaacaacg aataaaatcg acagaatcgt tcctacatag aaaaagagga
     5221 tacgccaagg cagacagttg attgcatttt tcaaattctt ttgtgggtca ctcgtctcac
     5281 caactgttaa tccgatcatt tcaattccag caaaactgaa taaaaccata ggaaatgaag
     5341 caatcattcc agcaaagcca aacgggagta attcattgag ctgtccaaaa tttgctttga
     5401 ttccgataga ctgggtatga aaatatgaga cgctcaaacc tatacccaat aaaataaaaa
     5461 aaacaatccc caaaatcttg atgattgaga aaaaagattc caattcgcca aaccatttga
     5521 cggcaacaag attgactgcc attaagatca ttaatgcact acctccgctt atcaaagctg
     5581 gtacatgtac aaaccagaaa tggatataca atcctattgc tgtcaattcc gcaagtccag
     5641 ctccaagcca gcaaagccaa taagtccaat tgacaaaaaa ggcccacttt tcacccaaat
     5701 agtagtcaat gaaatcactg atagagttaa atttactatt tgctaaaaac aaatcaccca
     5761 tagcccgcat caagccataa atagccacac cgcatatcaa atagacgaga ataacaactg
     5821 gtcctgccag tgcaattgcc tttgctgaac ctaagaataa tcctgttcca attgcgccac
     5881 ctagtgcgat taactggata tttcgattag ttagagtccg tttcattgcc ggtttttcca
     5941 tcaagttcac ctttttcttt ttcaagcagc atggcgactt cctgttccca agaaggtttc
     6001 tttatttttt caatacatag tggtactaca aacaaaatga cactgatggc tgtgactgca
     6061 attagattga tccatgttcc agctttcatc gtcagtccga cagaaaagcc gattgccgca
     6121 aataaaacaa taacgatcgc ccagacgcca agattgcttt tgttctcttt ttttccaatt
     6181 ctgaatggac ggttcaactt tgcatgtgta taacgtaatc tcaaaaccga gatccccata
     6241 atgacataga cgatacaata gattacagta gttgaattaa ctagcatcaa aaatgcttga
     6301 ttgacgccag gaatcagcaa ataaaaaagt gcaaagattg agataatgac agattgagtc
     6361 aaaataacgg atttggatac gtcaaattga tttgtcttcc aaaaattgaa ttttggtgga
     6421 tacgcacctt ttctagcact ttctgtcact gtctttgcag gtcctgatgc ccaagcagat
     6481 aattggacgg ccacaccgat gaagacaagc aaactaaaca gattcacaat aagatgcggc
     6541 aaaccgagaa tttggcaata aatcgaaata gattgtgcga tattattcaa ttccatttga
     6601 ccttttggta cgacatttgc tacaactaaa gcattcaaaa tattgacgaa aaatataaaa
     6661 atcagagcag caaatactcc gcgaatatac gttttcactg ggttcttcaa tcgagtgata
     6721 tacacggagg acatttcgat gccagtaaaa ataaacatga tcggcgcaaa ataaacaaat
     6781 gaggatgcag tcgtactatc tggtattact ttgtcccatg aaaaattgcc taatgttccg
     6841 gttggtacga ttccaacttt gatccacgca gcaagcccta ataagagcat catcgtcaaa
     6901 ggcacatata gacctaacca aacaccgatt tttcctccga tttttgccat gtcgaatgtt
     6961 aaattcaaaa aagtgatgat ccaataaaca actaaaataa caactaatgt aaatttgctg
     7021 ttcagcccaa gcggcacatt atcgataata ttaccaaaca acggagcgag tgctgaagca
     7081 accatcacca tccctggaaa catttggacc cataacagcc aggagacaat gaatccccaa
     7141 ttttttccta gagcattcga tacccaaagt tctggaccac ctttttgagg aaacattccg
     7201 gcaaattcac cagagatcaa tgagatcgga aaggcatata acaatacggc aaccagcatg
     7261 taaaaaatca tcgtccagct cgttgctgcc acatctggaa tatttcgtac acttgctaca
     7321 agggcacagg tcatcccgat aaaggtcatg gtcgtgattt gttttgatga accagcatct
     7381 ttcatcttta ttccttcttt ctttcaatag cgacatgttt ttcgttttct tttatttttg
     7441 ttcaataaca aaagtgacaa ggcttcatcg attcagtaca aaacccagta tcaccgaaac
     7501 atccattgaa cgaacttagt gaactgggtt tgatacgtga aacgaacgaa ccaactatag
     7561 aaagatttat aaaaaagaat gtcttgctga ggaatatatg aaaaacaggg ggaaatacaa
     7621 ctgttttgat gaggcaaata attgtctgtt ctccatatgt ctcttgctat tctttcacac
     7681 aaaaatcagt tctatttgat gatttcgttt agtttctttt ccattgcttt ggcgatttct
     7741 tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat agccgcacgt
     7801 agcagagtga tttgctgttc tttttgccat tcttctttcg atagacccat tctttcaata
     7861 aaacggatcg gagaatcacc gtaatcttct tgtgtaaatg tcgtatgaga agtaataaac
     7921 cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt ttcatttaat
     7981 tcattgatag cgttcaaatc tgtacaatct tgtactttaa atacccaatt gaccatatta
     8041 aaatcaggat gattcagtgg atagacttcg atcgtttttc cttttacagt aaagtgtaat
     8101 tgttctaaaa attctctgaa tctttgagct gcttcgattg atgcaccaat caattgacca
     8161 tatccggtca cattcaatgg caatacacga tgagctgtcc agactgcagc agccgtcgct
     8221 cctgcttttg agccttctaa aatataagca cctagcatat ctggtgcttc gactgatttt
     8281 tcaaacacat atggtgcaaa ataagaaatg atatttctca tatttttatg tttgattacg
     8341 attcctccag cagcataagg gacatagccc atcttatgcg gatcaatcgt gatagaatcc
     8401 gcttctgaaa tagctctgaa tccttcatac acctctttgt cgatcgttac gtaatggtgg
     8461 aatacatgat gctcttcaaa gaattcagct aaggaagcat atggcacaaa ttctcctgct
     8521 tcgtttaaaa acagtgaacg cgcgtacccg ccatatgcag cgtcaacatg taaatagaaa
     8581 tagatacctt catcttttaa cttttctctt aactggatga ttttatctac actgtctact
     8641 tgtccttctt ctgttgttcc tacgacagca accacaccta gaatcgggat tttttgatca
     8701 gctaattcac gaattgtttt ttccaaagcg ttgatatcca ttcgataatc ctcttgaact
     8761 gggatagcaa ccatctgatc taagccgact ccgcaaatat ctaacgcctt catccaagaa
     8821 tagtgttttg tttgaggaac cagccatttc cctaattttt ggatattctt tcctctacga
     8881 gaagaagcgg ctttgacctt atccatctct tcatccgtaa agctttcggt catttccaaa
     8941 atttcttcaa cagacagatt caaaagagcc cactcagaca tgtttttcac tttttcaggc
     9001 agaatttctt tgacagctaa aggaatcgat ttgatgcaac gggcgtacca taatccttca
     9061 aggttagcaa tagaaccatc tgctgtaata tgtccccatc cgtctgccat gttgaatagt
     9121 gaggcaaaat cctgtcctac ttctgcttcc atctgcgaag ttgccataga agattctagt
     9181 gcaacgttat tgggattcca tagcatcgca taattgtaag ctaataaagc aggcatcagt
     9241 gtttcagcgt tcatttgtcc ccagtaacgg cctgctgaat gccaggggat cgatcctgcg
     9301 cgtaatcgct gactgacttc atccaagact tctatcatat ggtcgcgggt ggctagataa
     9361 tctggagtta gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat ataattttct
     9421 ctccagccaa gatgttcatc taccatcttg ttcaacaaca ttttgtaaac tggtccattt
     9481 tcagctttgt cgcctataaa gactgccttg atatccatat ctttcatgtt tcttccctcc
     9541 atatttcgtt ggtcaatgtc tcgcagatac atgtgcttat ctgtaacacc tcttcactat
     9601 agcgccaaaa atgagaaaat tttttttata acatcagatt cacgtagaat atttcaaaca
     9661 aaaataaata aagtcggatt tttctgataa ttgaacgtgg aaaaacgaac aattttttaa
     9721 taaatcagac ttctaaaatg gatagtctaa aaaaaacgaa caaataactt aaatgaccat
     9781 cattcattca tcaactgtat ttgcattcct tttgcttgtt agaaattcac aatctatttt
     9841 ctaatgaaaa tatttatttt tcaattttta taacataaaa atctttcatt aacggaattt
     9901 acaggatttt tcagaaatta attgatgatt tccaaaagaa aacatgtcat aatattttcc
     9961 atattgtgca aaaaaataca agatggaggc tttactatga ttgaagaata tattgagaag
    10021 aatattttga gacagctgtt tttatgtgga caattctatg taaataaaga agtcaatcta
    10081 gaaaagctct ccaatcttct acatgtttgc aaaaccactt tactcaatga tatcaataac
    10141 ataaaaaaag aatttgaaga acaaattgtt tatacacatc gggaaaagga ttgttacact
    10201 ttatattttt cagaacatat acctcgatgt aagatcatgc agcaattaac ccaaaattcg
    10261 ttgtttttaa aaacttgcct gctttatttg gaagaagatg aacctgatta tctccaatta
    10321 actgagtgtg aattcatctc tgtcagcaaa gcttactcct taaaaaaaca agtattggct
    10381 tattttcatg attgtgggat cgaaatcgac cgctactccc ctcgattcac tgaaatggaa
    10441 agacgtctgt tgcttcttaa tgtggcttat cgtttaggca gctttaagtc ctgggagcta
    10501 cctgaatcgt tttttgaacg tgcggatcgt ttcattgaat ctgtaacgga gaatagcggt
    10561 agattttatg ataaagaaaa taaagaaatc ttatcgattg gttttgctat tagtttcttg
    10621 cgccaacagt tgggtactgt gacaatagat ccaacattta tcgaagaaat caaaaaacgt
    10681 cctatttaca attatgtcga gtcaacatgg gaaaccactg atttccaaac ctattataaa
    10741 aaagaagaat ttgtctttat tttgacactg ttcaatcttt gtaattatgg tttccattct
    10801 tatcagttga ttgcggaaga ttttcaacag ctacaccaag tgtttattga taatactccc
    10861 gaaataaaag aactagttgc tacctttgaa actcatttcc gtcaagaatt attcgggaat
    10921 cagccatttg aacgtgcact gattcatctg atgcgttcgg cttgggataa ctatcaatta
    10981 ttcatgccgg aaaaatttta tcttctaaat gaggaacagg caaatttata caaagacgtc
    11041 caaacacttt tttccagttg ggccaaacgg ctaccttatg atctacggtt taatcctaat
    11101 tgcatgcgag cttttgtcat cgaattgagc ggaattctcc gtctgacaaa agaacgtctg
    11161 actatctata tcgtcacaaa ttcggatgtt cattatctga tttatcggga agccttggaa
    11221 gctgtaacta ctttcgattt tcaagttgca ccaacaatct atgcaagtat atctgatata
    11281 aagaagtatg cacagcaacc ctcgaaccga gtgttatgtg aacgtacatt atatacaccg
    11341 gatgcagtcc aatatgagaa tattattcct atttcaatca atacgatcga acgagcaatc
    11401 atttcagctg tgcaaaataa ataagaggct gtgataaaca tcatgtcaca gcctcttatt
    11461 ctgaataaac aatattccaa aagtaacttc ttcggaaata agccgaaatt ttcataaatt
    11521 tcttcttatt ccttgaatct gcccacttct taacaacctc tcgataaacg gcgggctcat
    11581 agcctcactt atttgggacg tctactggcc cgatttcttt atttaaaaaa taagaaatga
    11641 tcgttcggat aactaccaat gcagctagct ggaaaatatc ctgcaaagta ggtttgataa
    11701 tcgattcgat gatcccagcc gaaacaagaa tttctaaact taggagtata taacctccta
    11761 acatcttttt aatagcattg ttctcgctga ctcggtcgtt tttcgcttct gtatggacaa
    11821 ataagtattt caagaaatcc ttaatcgaca gacaaacgcc ccaaatcaat aaacagatag
    11881 caaaaatatc aagtcccagt acaagttttt caaaatatgg aagaagaaac tgcatcaact
    11941 ctgtcgtcat cctatctctc ctcttctttg tttctctttg tcttctattt cctgattcaa
    12001 gaaataagaa atgactgttc gaatgataat aataagtgca agcttcaaaa tatctgtcca
    12061 agtcggcttg ataactgact cgatgatatc agcaacgact agcacttcca agcttaacaa
    12121 aatataactt gctaacagtt ttcttgtatc tgcattacga gtattacgtt ctgcatagtt
    12181 tttcttgaca tgagaccaat caaaaagttt gaatagaaca agaacgatcc cagcaactaa
    12241 tataccgata gaacaaatat ttaaaatagc aatgatccat tctagaaatt caatcaaatt
    12301 tgagtgaaac atataagcta cctactttct tttgatttgt tcctattctt ttattgtaaa
    12361 agaaagagac cttgttcgtc tatttaaaag ttgttttatt gatcacacct tgcctaaaat
    12421 cctcctcttc cctttctcat tcttcaccct ccataccatc catacatcat ggaagtatcc
    12481 aaaacaacat agcaagttaa aataaggctt agtccgtcat cagcttttca aagtggcact
    12541 gtctcgcgct ttttttaccc aaatttctaa aaatagaacc atacatctcc tcaaaacttt
    12601 tcttctttcc taccctatta ctaattacag gaaaatcaga cacttcttct tttttgtcaa
    12661 aaattccttt ttcttctcac cctatcattt ggcattctca taaaaacgaa ctatccttgg
    12721 atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca
    12781 tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaaaaca gggctattcg
    12841 aagatgacag tcatttatat gatgaatttg aatccatcta gcatcttatt tcgttgaatc
    12901 actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta aagcaataat
    12961 gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag aaggcaaatg
    13021 gaaaggggac cagatcacct tttattcatt ggatgatcac ggttcaaatg atggtgcacc
    13081 gaccgacact gtagtggatg aatattggaa taaaaatcat ccattagaaa accaaaaagg
    13141 ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt tgatggatct
    13201 attaagtcga ataccttctg ttactgagcc aggaatgact gctgaagaag atacgagaga
    13261 ttttgatgca aaacaccgta cctttgacaa agcacgtttg atggaaggtg gtaaagggat
    13321 cattgacggc ggaaaactgg gcttcaacaa caaggaccgt attcttctga ctaaactgat
    13381 tgccatgcct gattcagaag aagaaaaact agacaatatc actatcgaag aatatttcaa
    13441 agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa catttgcttt
    13501 cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga tttatgaatt
    13561 cactcaaatc gaacatttag tgggcgtcaa tcgaacacgt tacaatcagt acgaaagtat
    13621 catgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt tgaaccgccg
    13681 tgtcactgac tgggaattca aagatacacc gatgcaagat gaaatcactg tcactggttt
    13741 gaaaatgact aatacattga ctcaggaaga ggaacatgtc acggtagatg aagatacagc
    13801 ggtcattttc acgaatggtt ccattacaga ttcagctact ttgggagatt acgaaacacc
    13861 agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag ccagcgaacg
    13921 tttctataac ttaggaaatc ccgacaaatt tttcgcggat cgagatgcga gcgaatgggt
    13981 cagctttaca ctgacgacga aaaaccacct gttattgaat gaaatcaccc gtatcacgac
    14041 tcaagaacca ggaaatgcct tgaattcatt tatttcaact acaccgatca cgccattagg
    14101 ccaaaaagat gtcaatatgt ctatcgtcgt gcatcatcaa ccgcatttta cgtctcaaaa
    14161 accgaatgaa acagttattt ggggttattt cctttatcca agacgacgtg gcgaatttgt
    14221 ggataaacag tatattaaga tgaacggaaa agaaatgttg gaagaactga tcggacaact
    14281 ttcaaaagta gatcctggtc cagtgaatat tcgggaaaaa gaaacagaga ttttcgacag
    14341 cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcattattta ataaccgtgc
    14401 aaaaagtgat cgaccaaaag tcattcctaa gcattcaacc aatctggcct ttacaggaga
    14461 atttgttgag cagccttatc agatgatctt tactgaacaa agtgctgtac gttctggaga
    14521 aatcgctgct ttccattttg cgggcatccc tatgtcccgt cttgtcaaaa caccgcgtta
    14581 cgacaaagac atccctactc tagctcgtgc cgcaaaaaaa atgtttgaat aatcaagagg
    14641 ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc aagcaaaaac agctacttat
    14701 gtttttgact tggttggact tattatcgca gatgcaagct tttgaacacc gtttatcaag
    14761 aggttgtgaa aaagacgttt aatttcgagt attaagaaaa attttgtgaa aatggttcct
    14821 cacatttttg cataatttgg cttaataccg agaaactggc ttttgaacac cgtttaccaa
    14881 gaggttgtga acctaccgtt tagctctgag tcacaagaag caatttcaaa aaatagcttc
    14941 ctatgtttta tgaatattgt gaccgaggag ttggtagggg aaccccattt attcataaca
    15001 agaggttgtg acagatttca tgtcacaacc tcttattatt tatatccgtt tctttttgaa
    15061 aaaacctttt tttcagtatt cactctcttg ccaatccacc ataatccagc tgctatcact
    15121 acttcaacga ttgctactac actaaaaaat tggatgaatg acaaaatata tgtgtctaac
    15181 aaatgttcga gtgcttgggt tcgaatatct aactgactgg ccatatcgta caaatagaga
    15241 ctgactgaca gacttaataa ttggaagccc gcacctccta tcaccactgc aaatgaccaa
    15301 agaacttttt tccaagacat tctgtataaa taaaacactc cgctcagcaa tacaagcaag
    15361 atactactga tcattggaag aaaagaaacc attcgctctc gtatcgtcaa tatctgatcg
    15421 atgaacgaca tcaaatagat tgattgtatt cctcgtcgaa aaatcccaga agtcatatag
    15481 atgagtaaat tgttttcttc actagcaaat ggatcatcct ctgcttcttc ttgtttgtat
    15541 gtattgatta tctcttggag cttttcttct acttcctttt ctccaatcag ttctactggt
    15601 tctttttcat atctgttgcg gatgaaaagc tcgatatttt tttgtactag ttcttcagaa
    15661 acaatctttt ccaatgcttc agccggagct ccgatctcat aatgatagct ttggatctct
    15721 cgattgatct cagcagctac ttttttcgca tagtccgatt gcatgttttg ctctaacata
    15781 aaatgttgat ttgccaaggt gcttattaaa caaagcataa cagtcaggca aaacaggcag
    15841 acagaactaa tcataacaat gatccgttta ttccatttat tttgaatttg tttcattacg
    15901 cctcctcgta tttgtttttt cactgaaacg aatgatggtt ggttattgtt taaatagtta
    15961 aaatgattca atagttcaac tatactttac tgtacgtaag gatacaatga aggcacactc
    16021 cttctttttc taataaaaaa aaattttgga actcttgctt ttttgaggat ttttattttg
    16081 agaaaaaagc gtttcattat caaaaagata agaactcctt tagaatgaaa ctatctgtag
    16141 taccaataat gcagacaatt ttgtgaatct gaaaggagca tctccaatga aagcagtcac
    16201 ttggcaagga aaacaaaaaa tggaaatcag aaacgtagat gatccaagaa tattagaacc
    16261 aacagacgca atcatccgta tcacagcaac cgccatttgc ggctccgatc tgcatcttta
    16321 ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggtcacgaac ctatgggaat
    16381 cgttgaagaa gtaggatcac aagttcaaac attgaaaaaa gggcaacgag tggttatccc
    16441 ttttaatatc agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga
    16501 tgaaacaaat ccagatcagt tatatggcgg gcttttcggt tttggcaagt tgaatggcaa
    16561 tcattggggt gggcaagcag aatacctaag agtaccttat gcagactcta cgtcgtttgt
    16621 cgttccagat aataatcttc ctgatgaaaa agttttgttt ttatcagata ttttgcctac
    16681 agcttattgg agcgttgaaa atgcaggcgt aaaaaaaggc gatacagttg tgattctagg
    16741 atctggtcca gtcggtctat ttgctcaaaa attcgctgta atggctggag cagagcgcgt
    16801 gattgcagtg gatccagtac aacatcgttt agataaagcg gctaattaca acggcgtgga
    16861 aacttatttc ttagaagaca cagcacaagc tggaacggaa ttgtatgagt tggctaaagg
    16921 tggtgccgat gtcatcatcg attgtgtagg aatggatggg ttagaacctg taaaagaaaa
    16981 agcaaaaaat ttggtgagcc tacaaagcgg cacgatttct ccattacaga tggcttcgca
    17041 agctgtcaaa aaattcggaa ccgtccaagt aactggtgtg tatatgacgc cagcttcttc
    17101 ttacccgctt caagagtttt tcatgcgaga tattgcggtc aagcatggac aggcaccaat
    17161 tatccatctg atgccgaaaa tctacgaaat gattgcagaa ggactatttg atcctacaca
    17221 gatcattact cattcgctgc cactagaaga agcagcacaa gcatatgaga tctttgacaa
    17281 aaaagaagat aaaaatataa aagttgtttt gaaaccttga aatcagtaaa aaatacaggt
    17341 gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga
    17401 agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaaccgtt acacattgat
    17461 ctcactagct gtcgacttat taaccgggat tttctatatt tttggaagcc ttgcttcctt
    17521 aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgctg ttttcttgac
    17581 gattcgtcct attctcagaa tcgttaggaa cattttcatt tacgacgaga aaagaacaag
    17641 gaaaaagcat gggaaggcag tttagccttc tccatgcttt tttgttattc ttgtgttgtg
    17701 catatttcgc atactgcgat tcgaaaaatt tctaagtata gaacaatttt tcggtttcct
    17761 tctctatata ctcacgtcta tgctgtttca tgtatctact cttacagcgt catttggtac
    17821 actcgcacag gaattccttc attatcctga gccatcccga taaactgcca attgtatttt
    17881 tcataaaaat ccgtatgatc cgtcacaagg taaagcacct ttacgcccat ttctgcaaag
    17941 tcttgcttga tcgtttctaa aatcattttg gcaatccgtt ggtttcggta gtctttttca
    18001 acaaacagcg cacagacatt cggagtcaaa tctactcgat catgaaaatc attgtcaaca
    18061 acacctgcac ctgcaataat ctctccccat tcattcaaca ccacatacca ttgcggaacc
    18121 gatgtgtttt ttctcgtgct tgcttggata ctttcttcat aaacagcaac tggcagatgc
    18181 cattttgaag caaaccattc ggctgtttct tttatcaatg tcggtcgatc ccgcaatttt
    18241 atgactgttc ttctctccat ttgtactctc tcccattcat atttggtcta ttatagcacc
    18301 ttgtatttca aatagctttc caatcgctcg atttttttga atgaatggga ttttttattt
    18361 atacacaaat ttcaagacaa atatacctcg attttctcat tcattattcg atcctaagtt
    18421 ttaaattact tatttccata aaaaaacaga accagataac cggttctgct ttcttttttc
    18481 attaagcatc caaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggca
    18541 gatagatcaa aatagaaagg atcagattga cagcggccag tacaccacct gcaatactct
    18601 tcgttgccaa tacaccgcca ataactggtg gtgttaccca tggtggcata acagtagctg
    18661 ctggcactaa acctgtagaa gtcgcaaagt acgcaacact aactaagacc attggtgtca
    18721 aaataaatgg aatgaacatg attggattca atacgatcgg taaaccaaac atcgttggtt
    18781 cattgatatt gaaaacacct ggcgcaatcg acaagtttgt tacaaccata tacggtttgt
    18841 ttttacgtcc tactaaataa atagctaaca gtaaacctaa tgttgca
//
LOCUS       DAPBBG010000053        18646 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPBBG010000053 DAPBBG010000000
VERSION     DAPBBG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.054"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(367..933)
                     /locus_tag="RD477_002166"
     CDS             complement(367..933)
                     /locus_tag="RD477_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7634496.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(933..1889)
                     /locus_tag="RD477_002167"
     CDS             complement(933..1889)
                     /locus_tag="RD477_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDT7634497.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1889..2545)
                     /locus_tag="RD477_002168"
     CDS             complement(1889..2545)
                     /locus_tag="RD477_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDT7634498.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2915..4933
                     /locus_tag="RD477_002169"
     CDS             2915..4933
                     /locus_tag="RD477_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7634499.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5003..5209
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5360..7774
                     /locus_tag="RD477_002170"
     CDS             5360..7774
                     /locus_tag="RD477_002170"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDT7634500.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8122..9675
                     /locus_tag="RD477_002171"
     CDS             8122..9675
                     /locus_tag="RD477_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDT7634501.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10056..11840)
                     /locus_tag="RD477_002172"
     CDS             complement(10056..11840)
                     /locus_tag="RD477_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634502.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11855..13576)
                     /locus_tag="RD477_002173"
     CDS             complement(11855..13576)
                     /locus_tag="RD477_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634503.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13676..14437
                     /locus_tag="RD477_002174"
     CDS             13676..14437
                     /locus_tag="RD477_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7634504.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15404..15672
                     /locus_tag="RD477_002175"
                     /pseudo
     CDS             15404..15672
                     /locus_tag="RD477_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16344..>18646
                     /locus_tag="RD477_002176"
     CDS             16344..>18646
                     /locus_tag="RD477_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634505.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         6129 a         3312 c         3765 g         5440 t
ORIGIN      
        1 ggggcataat ttgactgata ttaagttagc tattgatata tgaatgacat acaaaaatat
       61 aaaatctttt aaaaatgctt atatatcaat gtttttgcca ttcttttata tatctgttat
      121 atgtcaaaaa aatgtgttca atttcaattt gtatttgaat aaagaggttg tgaaaaagct
      181 gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgatttgg
      241 ttggacttat taccgcagat ataagctttt gagcaccgtg aataaagagg ttatgaaatc
      301 aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca tatcatttat gttttgcgtt
      361 ttttatttat ctttttttct caatacaata taaaataggc ggctggttct tttggttgat
      421 aaaccgataa ttcagcacat tatatgtttc ctgtggcagt ttggaacaaa aagaatcaac
      481 catatccagt tctttttctc caccttcatg accatagtag acgacaagga tcaatcgtcc
      541 gcgaggcaca agtcgtttca atatttcttc catagctgtg cgtgtagtct ctggcatagt
      601 gatgattgct ttatcacttt ttgggagata ccctagattg aaaatccctg ctttgagatt
      661 ttgttgttca gctatcactt ctcctaacgt ttcgtggcct tgtagaaaca atgagacacg
      721 ttcttctaac tgacgctgtt ctagcctttc acgagtagca tcgattgctt gttgctggat
      781 atcaaaacta tagacatgac cagtttttcc gatgtgttct gctagaaaaa ccgtatcgtg
      841 gccattgccc atcgttgcat ctacgacgtg atcccctggt tgcaagattt cttgcagtaa
      901 tgtatggctg aaatgcaaag cagtttgcaa catcagacgc tcaccttttt ccgtacatca
      961 tattttcctt ggaagctatc tctgcgaaga agttcttcat caattgcatt caagacttcc
     1021 cattttttca agctccacat tggtccaatc aaggaatccc acggcgcatc ccctgtcaaa
     1081 cgatggatga tgatctcttt tggtatcatt tccagctggt cgcaaatgac attgacatag
     1141 tcaggacggc tcattaattg aaggcgacct tcatgatagt cccgcaacat tctcgttttg
     1201 cgcatcagat gcatcaaatg gagcttgatt ccttggatat ctgaatctaa aatggtgcgt
     1261 ctgacatttt ctttcatcat ttctaaactt tcgcctggta atccgttgat caaatgcgta
     1321 caaacacgga tattatgttt gcgtaattta gccacgccat ctaaataagt ttgataatca
     1381 tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg tttgcaatcc aagttctacc
     1441 cataaataca tccgctcgtt cagttcagca aggtaatcaa ctacttcatc tggcagacaa
     1501 tccggtcgtg taccgactga taatcctacg acacctggta aattgactac ttgctcaaat
     1561 cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa aattttggaa ataaacgatg
     1621 tattgttgga catgaggcca tttttgatgc atcatgtcga tttctttctg gaattggatt
     1681 ggaagagggt cttcaggtgc aacgatcatg tctcccgaac cagatacact gcaaaaagta
     1741 caaccgccat gggcaacagt cccatctcga ttcggacagt caaatcctcc atcgatcggc
     1801 accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt aattccaaga atgatatctt
     1861 ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt ctttctgtaa gagttatcta
     1921 tttgttttct tttttctcta tacactggat aagtcaggca cagccaagaa agactgagta
     1981 agtagccgcc gatgatatca gtaggaaaat gaacgcccag ataaatgcgg cttatcccga
     2041 cacttactat aataaacatc atcagcaatt gaagcaatgt gcgtaaggtt cttttttgaa
     2101 caaaagctgg aagcagaaat agtaaagaac cgtaaagaat catactgcct gctgcatgtc
     2161 cactagggaa actgtaactt gtttcaacta ctaagtgcgc caatgaagga cgctctcgca
     2221 tgaaaaatag tttgatcacc gggttgatga ttccagcaat tacaataaat ccagaaacga
     2281 accaaaatgt ttctgccctt tttttcttat accacatcca taaagccgca caagcaaata
     2341 aaatgatgac ggtaagagga ttaccgaatt tagtcataaa gagttggtag ctgttcagat
     2401 caggataaga agctctgaca atgcctgtaa accactgatc gaaaccagaa agccacgaca
     2461 gatggaactt tacgatccag cccaaaatga aaaacaggac aagaaagaag ctaccagcca
     2521 cctgaaatga tgttttatat ttcatacaat tcctcctttg aagcaatgac ttattatagc
     2581 atttttggtc cagcgaaaaa aacggtaaag agataaaacc aatgtatgat tcctctttga
     2641 ataaccaata ttcttataaa aaaaataact gtaatcggaa agacattttt tggtaatgca
     2701 aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat ccttttaaac aagaacgtag
     2761 acgtaggaag aacttgaaaa tgaacagaag ttgttcatta cggtaaatta caatatgatt
     2821 acaaaaaaca acaaagctct taaataactt ttaataaata ttaagagaag ttataattat
     2881 taataataga taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg
     2941 gagacgttta gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg
     3001 aactgcgatg gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc
     3061 ggatgaaaca cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat
     3121 tgctacttat gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc
     3181 acaagcagtt gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa
     3241 tctttttggg ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt
     3301 tttaaataat caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga
     3361 atctttcaat gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta
     3421 tgccggtact tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg
     3481 gcgttatgcg actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg
     3541 attgacccaa tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg
     3601 caataatact ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc
     3661 aggttcagca acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc
     3721 aaataaattc ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt
     3781 catttatcca ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa
     3841 taattccggt aacaatacca actcttcagg aaatgctggt acatcaaacg gaggacaaac
     3901 aactggtgcg aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca
     3961 cggaattacg atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc
     4021 aggacaacag ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc
     4081 aaatactggg aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc
     4141 aggaacgaaa tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg
     4201 aattacgatg gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg
     4261 acaacaactg actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc
     4321 gtcaaccaat acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac
     4381 ttataccgtg aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat
     4441 ggatcagtta atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt
     4501 gatcgttaaa ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa
     4561 accaaataca ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta
     4621 taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga
     4681 tcagttaatc gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat
     4741 tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta
     4801 tactgtgaaa tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc
     4861 tcaactaatc gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat
     4921 tgtagctaaa taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt
     4981 tactataagg ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt
     5041 ttcagagagc gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt
     5101 gcccttgctg aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc
     5161 ttttcactaa ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc
     5221 tttttatagg atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata
     5281 aaaataaaca gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa
     5341 tagaacagga ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa
     5401 atactgggca aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta
     5461 cgcactggat atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg
     5521 ttatacggca acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca
     5581 tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa
     5641 cgatccagct gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt
     5701 aggatttagt tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg
     5761 gacacaatgg atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc
     5821 ggtaaactgg gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa
     5881 aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat
     5941 cactgcttat gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat
     6001 taaagaaatg caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt
     6061 ggctggtacg gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc
     6121 aacttactcg gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca
     6181 aaaagctgct gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac
     6241 ggatcttgca aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa
     6301 tggcaaggaa atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc
     6361 catcatggct gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact
     6421 agaaattctt ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg
     6481 tacgcacatc aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat
     6541 gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga
     6601 ctggctattc tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga
     6661 cggaacagtc acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa
     6721 catcaagcca agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt
     6781 cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc
     6841 aggaagctca tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga
     6901 ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc
     6961 ggtacttcat ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt
     7021 tcctacaaaa gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa
     7081 cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta
     7141 cggtgcagat acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc
     7201 ttggagcgaa aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat
     7261 cgttgatgag aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga
     7321 taaagtgtat catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa
     7381 tacagcaatc tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc
     7441 ttatgaatat atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga
     7501 agaactatgg gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata
     7561 cgatgaagca gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt
     7621 tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt
     7681 agccaatgaa atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt
     7741 accaaacaag ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat
     7801 gagttgtgca aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt
     7861 atttcaaatt gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt
     7921 tcaatgtctt tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt
     7981 ctattaatct tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat
     8041 tcataaattt tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa
     8101 gtatgagaaa aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct
     8161 aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta
     8221 agcttagtat ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat
     8281 atgagtgcag tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt
     8341 tcgtggggag ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg
     8401 ctccgttacc atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag
     8461 tttttacata ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg
     8521 acgagtcaag cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat
     8581 gatttaccta tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt
     8641 tttttactag aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg
     8701 acaactaaga tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc
     8761 tattatggga gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg
     8821 acaatgaact caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat
     8881 cctaagcaaa aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag
     8941 acgcactctc tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat
     9001 tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat
     9061 tggatggaca acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg
     9121 gatgatgtac gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc
     9181 acccaatcag ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta
     9241 tttatctgta catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg
     9301 gatggcggaa tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat
     9361 gtcagcttcg ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca
     9421 aacaacggag gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca
     9481 ggggggatag atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta
     9541 gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc
     9601 ctcttccgtt caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta
     9661 gaacaaggag attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa
     9721 acaaattcaa aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg
     9781 acaagagcaa caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct
     9841 tattgccgat aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc
     9901 ttttgtcact acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta
     9961 ttctttgtgt ttctagtggc accactagta catttaaaaa agctatcgat catggactaa
    10021 tcatacatga tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta
    10081 taaagactgg cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg
    10141 atatcgccat tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt
    10201 gcaatcacaa aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct
    10261 tctgttcgtg tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct
    10321 agaatgactc gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg
    10381 ctagaaatga tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg
    10441 atttcagccg cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta
    10501 tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt
    10561 agttctccac gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc
    10621 agttcataaa aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca
    10681 acgatcgcta ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta
    10741 tctgatgtgt aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa
    10801 agtcttgcat ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta
    10861 tcgattgctg ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt
    10921 tcagaaatct gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct
    10981 gatagaacaa gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa
    11041 cgaatggccg gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc
    11101 tttttattta cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc
    11161 actagattac cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga
    11221 ttctgttcag aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca
    11281 acgatcagca ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca
    11341 gcagcagcta ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga
    11401 gaaagttgat tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca
    11461 aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg
    11521 atcttttcgc ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag
    11581 actgaacagc cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa
    11641 gaaagctgtc caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc
    11701 atgatcaagg gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg
    11761 taaaaatatt tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc
    11821 aacgtttttt tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact
    11881 cctgataagt ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc
    11941 cttcatctaa aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa
    12001 tcacgagagt tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt
    12061 tatagtctaa agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg
    12121 ctcgagcaat acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa
    12181 caaagctgtc caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga
    12241 tcgctttaga caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa
    12301 ttgtcccgct gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt
    12361 cttgagtcat ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac
    12421 taagtctcaa gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg
    12481 ctgttgtttt tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt
    12541 cagtatattt aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt
    12601 cagctactgt tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta
    12661 tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca
    12721 agtaagcaag tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg
    12781 ctccttctgc tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga
    12841 gcaaaaaagc aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag
    12901 catagtcagc aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac
    12961 ggatcatatt aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg
    13021 gctggatact caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat
    13081 aaaaaacaac agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga
    13141 gaatggtgaa aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca
    13201 tttgctggac attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac
    13261 gactgatatc cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga
    13321 atccaaacct agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc
    13381 ctagaaacgc tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac
    13441 ttgctatacc tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga
    13501 tttctaaaca gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg
    13561 tttttaagat agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc
    13621 aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa
    13681 aaaaatgtat acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg
    13741 ctattgggat gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt
    13801 gtttgatatc gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat
    13861 accgatcaaa cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact
    13921 tgatgaaaca gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga
    13981 gataagggag caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc
    14041 tgtagaagag atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat
    14101 caaaaaattt ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc
    14161 acagacgatg tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt
    14221 gatttgggag aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa
    14281 ccatgcggaa cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa
    14341 aacaggagaa gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac
    14401 tgaatattat catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc
    14461 gaaaatgatg gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg
    14521 tgggggatta ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta
    14581 aaaatggaat ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt
    14641 atggaaaaat aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga
    14701 agaaaaattc atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg
    14761 tgcataaaag cattacactt aaaatacatt gttgcttaca ttattacaat atgttaataa
    14821 ggcaaaaata aatagatgga ttgaataaac atatttttta atcaaatagt ccctactgtg
    14881 actttcctca gctatcggtg atactaccga tagcattttt ttgcttataa aataattata
    14941 agtatttaga gatttttctt ctaacattct tcaaacaaga aaaatcacaa aggttattgt
    15001 tatttttata tattcttttt aagaatgatt gtttttttat agaaatttta aaaaataaaa
    15061 cacgttattg atggatagtt agtttgttat ttggtaatat tatagaagtt gactggtagt
    15121 gatttatttt tcgtcgtttt ataaaaatgt tattttttgg aaaaattcac aaatatgtaa
    15181 cgaatgtttt tctggaattt gctgtgttaa aaagcagtat gactaatata aatactattg
    15241 tcatttatgc aattttttat ggatagtaat tttagagggg aatcaatgaa ggaagaatag
    15301 aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata gcaatacatc ttcaagcttt
    15361 aaaattcgag agaatcatct tggtagtgga atcaactaca acaaaaggat taaaccaatt
    15421 aatagataga attaccaatg gaaaaataga aaaggtagtt attctataca aaagttgttt
    15481 aattcgcttc ggttatgaac ttagtgaaac cctttgcaat atatggtaca gaatatagaa
    15541 attattgaca ttacagaaaa tgatgaagat ttaatacaga ttgtcactat ttgtagatac
    15601 cttttacaag gaaaccgagc caataaagct aagaagatga ttaaggagtt gatggaagac
    15661 gatacaagtt aagaaagtga aactacgccc aaataaagag caaaaagagt aattggacgt
    15721 ggaaaatgca agaaatgaac catcgattcg gtggacagtt tattagtaat gacggtttga
    15781 gaacatccat gacgagaatg aaggaacatc ctaaatgcgt ttagttgaac caagtgccaa
    15841 ataatatggc caaataagcg atcagttatc gatgaatccc aagtattcta aactatatat
    15901 tcattttgat gctaagcatt gatatatttt tttgggattg atgaaggtta accaatacag
    15961 ggactcactg atgaaattct agagtctgac cttggtatca acgatttagt gattgtttct
    16021 aatggaaaag tataaaagta tataagtaca ttaacaaaat aactattagg gttacgctcc
    16081 agcttgtgca aggcactggc aggaggaatc gtgaaatcat aattatggtt aagccacacg
    16141 gacaactaaa agtcatacaa agctatcgaa tcaatcaaca gaagacaatc ggcaatcatt
    16201 agctttgttt acagcttaca ttgcaacgct tgagttgaac cgaaaatacg agggaggtaa
    16261 cgcagccatt ttgagttggg gaatcttttt agaaaattat cgggtacctt gttgtttagt
    16321 caaaattata agggggagta atagtgagga acaaatttca taaactgtta agtatgtttc
    16381 tagttatgct tattgtgatc aatcaatttg ggaacataac ggttctagct gagtctttag
    16441 cagaacagcc agagcgttcg ctgacgatcg aagttgatca agaggaggct gctttgggtg
    16501 agcagttcca gttgcgagtc agaggaacac cagacctgct aaatgcattg gtcattgata
    16561 agagtgagga tttgattgaa ggggaagtga agcaactcaa tgattacgaa cgtctgatta
    16621 cttattcttc ccagcattca ggtaaatacg aattgaccgc aagaagcggt gatattcaat
    16681 caaatacggt ctcaatagaa atcagtgtac cagaagaaag cacaagtgag tcttttaatg
    16741 taccagaaga aagcacaagt gaatctttta atataccaga agaaagctta agtgaatctt
    16801 ttaatgaagc cagatcatct gagccatcta taacgcagga atctcagatt gaaagacaag
    16861 aggagctctt gaattatgac atcggcatct ctgctgagcg ccccactttg ccgagcaggc
    16921 atacacaaac gttaaaggga tacattcaag gaggcgctgg cgatattctt gaagaatatg
    16981 gggagatggt cgtacgagtt cctagagcat cgattggctt aattgagttc attagagtgg
    17041 aggtcgctga tgattcacct ttaaagccac tggcatttta ccaagaaggc acagacgttg
    17101 tttttccatt tacctttgat tatgaaggat taaatgaagc cgctcagtcg tatacattca
    17161 gtatcacgtt tgaagttgct ttctttcaat caggcggtac tgagccaaat aatgactttc
    17221 aagtgagtct tacttatgct gagcgagcag caaacacgga attttcaatt acgcctattc
    17281 cttcatcaag acccattttt tcgaaattat actatggctc tagagaaatc gacagccaag
    17341 gaaatcttgg gaatctgctg attgacgggc aagacgtgac caacagtaat aaatttattt
    17401 tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt ggttaccgat gtactaccgc
    17461 aaggaacaac tttggcaccg aatccgacta catggagttc cactggaaat agagaaccta
    17521 tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg tgaagcaaac gttagaactc
    17581 attatgtttc cgaggaattt gtggacaaag tatcttttaa taaggaaacg aatacacttg
    17641 ttgtggactt tggtaatcta gcagagggtc aatcgtattt tattgaatat gctcttgaag
    17701 tgaccgatgt cgacttgttc aatgaaggaa gacaagagga tgggttttct cggaatcgag
    17761 cgagtatgac ctacaataat caggtaatga gccgagacta ccgcttgaga atccctgaaa
    17821 gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa aaatgttatc aatctcaatg
    17881 aaaatgaact aacgtatact ctgcatgtct cgatgttttc tggaacaaga aatacgcttg
    17941 ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc atttctatca gatattccct
    18001 ctttcattga tgccgaagtc aataatggaa tagttcgttt cagtgcgaat aatacgattg
    18061 gtggtccgac aagttttacc ctctctttta aggtcaatgt agaggcgtat gcggtcggcg
    18121 atcaaatttt aaactttgca acgcttgtga atagtgacaa tcgattgaat acgagtactg
    18181 taagaacaag gaaaatcgat gggcgtatta aagtaattaa aactgatgag aatggccaaa
    18241 ggctggcagg agcaaaattt gaaattttat caagcgattc acaagtgata caagaaggta
    18301 taacggcaga aaacggagaa tttttaagtg aaccgcttcc tgtaggtagc tatcaagtcc
    18361 gagaaacaga agcaccggaa gggtatcttt tagatgcgac ggtacatgat gtttttataa
    18421 atgaatactc catagaagaa gtgacgttaa ccattgaaaa catccttgat acgggtggtg
    18481 ttgaactaat taaacatgct gaaggcagtg gtgaagtcct tcaaggagct gtttttgagt
    18541 tacagaaccg agaaggagaa acgctacaaa ccggtttaac tacaggagaa gatggaaaac
    18601 tggcaattga cggtttagca ccaggagcat atcagctggt agaaac
//
LOCUS       DAPBBG010000054        18106 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPBBG010000054 DAPBBG010000000
VERSION     DAPBBG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.055"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..409)
                     /locus_tag="RD477_002177"
     CDS             complement(152..409)
                     /locus_tag="RD477_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634506.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            572..1546
                     /gene="bsh"
                     /locus_tag="RD477_002178"
     CDS             572..1546
                     /gene="bsh"
                     /locus_tag="RD477_002178"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDT7634507.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            2305..2622
                     /locus_tag="RD477_002179"
     CDS             2305..2622
                     /locus_tag="RD477_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDT7634508.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2825..3190)
                     /locus_tag="RD477_002180"
     CDS             complement(2825..3190)
                     /locus_tag="RD477_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634509.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            3398..3880
                     /locus_tag="RD477_002181"
     CDS             3398..3880
                     /locus_tag="RD477_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634510.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            4077..4967
                     /locus_tag="RD477_002182"
     CDS             4077..4967
                     /locus_tag="RD477_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDT7634511.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWEIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            5292..7766
                     /locus_tag="RD477_002183"
     CDS             5292..7766
                     /locus_tag="RD477_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7634512.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(7869..9605)
                     /locus_tag="RD477_002184"
     CDS             complement(7869..9605)
                     /locus_tag="RD477_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDT7634513.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9602..10306)
                     /locus_tag="RD477_002185"
     CDS             complement(9602..10306)
                     /locus_tag="RD477_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634514.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10437..10940
                     /locus_tag="RD477_002186"
     CDS             10437..10940
                     /locus_tag="RD477_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDT7634515.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10900..11238
                     /locus_tag="RD477_002187"
     CDS             10900..11238
                     /locus_tag="RD477_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDT7634516.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            11259..12677
                     /gene="ffh"
                     /locus_tag="RD477_002188"
     CDS             11259..12677
                     /gene="ffh"
                     /locus_tag="RD477_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDT7634517.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12789..13367
                     /locus_tag="RD477_002189"
     CDS             12789..13367
                     /locus_tag="RD477_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDT7634518.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13371..13892
                     /locus_tag="RD477_002190"
     CDS             13371..13892
                     /locus_tag="RD477_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7634519.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13971..14525)
                     /locus_tag="RD477_002191"
     CDS             complement(13971..14525)
                     /locus_tag="RD477_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDT7634520.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14778..15053
                     /gene="rpsP"
                     /locus_tag="RD477_002192"
     CDS             14778..15053
                     /gene="rpsP"
                     /locus_tag="RD477_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDT7634521.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15065..15316
                     /locus_tag="RD477_002193"
     CDS             15065..15316
                     /locus_tag="RD477_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDT7634522.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15441..16232
                     /locus_tag="RD477_002194"
     CDS             15441..16232
                     /locus_tag="RD477_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634523.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALRMAVSMKRL"
     gene            16402..16923
                     /gene="rimM"
                     /locus_tag="RD477_002195"
     CDS             16402..16923
                     /gene="rimM"
                     /locus_tag="RD477_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDT7634524.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVGLTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16913..17674
                     /gene="trmD"
                     /locus_tag="RD477_002196"
     CDS             16913..17674
                     /gene="trmD"
                     /locus_tag="RD477_002196"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDT7634525.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17810..>18106
                     /gene="rplS"
                     /locus_tag="RD477_002197"
     CDS             17810..>18106
                     /gene="rplS"
                     /locus_tag="RD477_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDT7634526.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYY"
BASE COUNT         6068 a         3116 c         3591 g         5331 t
ORIGIN      
        1 aaatgtaaag tcttatctct tttcttaatg ctataaattc ataataacat tatggacaat
       61 aaatttaaaa tttattcaga ctgtagacaa actcctaaat ttagagtgtt gtttgcagtc
      121 tgacgactca aaaatgagtc gtataaatca attatagtaa taagtcattg atttctttaa
      181 attctttatc aagttctttt tcatcatatt tttcatattt atcagcttca tgaagaactt
      241 ttttaatttc atgaatagcc tttcgttcaa ctaaccattt ttttaggtta tgtttttttt
      301 ctggattttc agataacgta tcaagttcgt ctaactcttt atccagttta ttgacaacag
      361 aaacaatttt gtttacaact ttttcttctt tgttttctaa atttgacatt tatcttcacg
      421 tcctttttat ttgataattt aagtatagaa ctttgatata gtttatacaa ataaaaacgc
      481 ttctttgtgt attctgtgct tttcttttgg aatgaggaaa actttggagt aatatgaacg
      541 tggacagaaa aatatttagg aggaataaac tatgtgtacg tctattactt atgtaacaag
      601 tgatcattat tttggaagga attttgatta tgaaatatct tacaatgaag tagtcactat
      661 tactccaaga aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc
      721 aatgattggt attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga
      781 aaaaggattg agtatggctg gactaaattt ttctgggtat gctgattata aagaaataca
      841 agaagggaaa gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc
      901 aacagtagga gaagctaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag
      961 tgacgaactt cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt
     1021 cattgaaagt atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa
     1081 taatccttca tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga
     1141 aactcctaaa aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg
     1201 agggataggc ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac
     1261 gaagctgaat tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat
     1321 cttaggttca gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata
     1381 tacaatttat tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga
     1441 agacagtcaa attactgcaa ttgatatgaa taaagaagac ttagatagtc ataagttaat
     1501 tagttatcca attatagaaa aacaacaaat taaatatata aattagttaa tgtgttgtga
     1561 ttgattattt aatatagata taatacaaaa aggcaaagat attttcaaaa cattttttct
     1621 tgatgtgata tccttgtctt ttttgtaatg tttttaactt attgcttttg caaaatttat
     1681 ttacctgttt tctggatcaa cgatagtgta tattatgcta tccttgtttg gattttagga
     1741 cgtttaaaga ggcacttttt tacataactt aatccataga ccttttaagc atgctgtgag
     1801 ataataataa agaagagttt aaagcgcacc ccaaaccact tccccataag tgtattacgc
     1861 tttaaactct tttatatttg aattcactaa gaagcatacc atatatttga attcactaag
     1921 aagcatacca tatttttgaa aaaaagtgag aaaaaaggct tataattagg atttagagta
     1981 attaattaga tgtttatttt ataggactga tactatagat tacaaatagt atcgtatttc
     2041 gcaatcttaa aattatcttc ttactgtgaa taagataata aaatttgtat ttgtcctttt
     2101 taggtaactt attttaatct atataccttt ctggatcaac gaaagtatac tttatatagt
     2161 cataacgccg atggtaacta tgcactgtaa cgataatgct gctaaaatat ctttttacga
     2221 aaaacatcac aaggaaaaac ttaactatgg tattatctaa acataatttt ttcggtattt
     2281 aatatttaat taggaggtaa tattttgctt ttttatagta attttatctt aatagtagcg
     2341 atattattac ttctgaatat atggattttt gataggagta gaaatgcaag tataggtttt
     2401 agaactaaac gaagtctgtc ttctaaaaaa aattgggtat actcacaaac tattttttat
     2461 ggaggaattg ttttaattag tttactttct tcaacacttt attctttgaa tattatagat
     2521 gtttccacct caaattcaat atctattatt ggaatcatta ttgctgcaat tattacacaa
     2581 ctgtttttag tgtttggaga gaagaaaaga agtaaaaaat agaaaaacta gtgatagttt
     2641 tggtgatagt gatattaaaa atcagaaaag atagaaaaag caggagatag ataaaatgct
     2701 gatatttcag catttttaag agtataaaag agaaaaaaag taataaatgt atacatgata
     2761 taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca tatatggttt
     2821 gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac gacccaagga
     2881 tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt tttaaaggat
     2941 gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg acagtagata
     3001 taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa tatccagcaa
     3061 aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga aaatcaagca
     3121 gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt ttccagcaag
     3181 tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt ttattgtaaa
     3241 ttgaatcctg caataatcaa ttactttttc attatttcta gattaaatga ataaactctc
     3301 atgccattat ctctaaaaaa aaagtttttc cccctaattt ttttagatag aaaattgtat
     3361 agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag cagtaatcga
     3421 tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta catgggtaaa
     3481 tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat ttgtggtttc
     3541 taggaatttt ccagctttga ctgttttcta tttagtgata agtgtaggtc tcttattacc
     3601 aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct tattagtatt
     3661 tacatctatg ttttctttgc tgcctcgctt gattatcggc cctaatggtt tgagcgagag
     3721 tggattgaag gattcagctt attcctccat gtatttcttg acgatcaaac catggttcta
     3781 tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag aaatcaggat
     3841 aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca tcagtaaaaa
     3901 agcatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat tatgtaaaga
     3961 atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca gcaaccatac
     4021 gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc aataaaatga
     4081 aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc ggctcaggat
     4141 caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat gaacaagtaa
     4201 aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca caagaaagct
     4261 ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc ggaactggtt
     4321 tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt gcagaagaaa
     4381 gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc gggatggcac
     4441 caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg attgatatct
     4501 ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa atcaacgtcg
     4561 tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga ctggatggtg
     4621 ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag ctggtaagtc
     4681 aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc acacaagcac
     4741 taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc tgggaaatct
     4801 ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa aaattcttag
     4861 actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc tatttaccaa
     4921 tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta gcagataaac
     4981 atgccacggt agtcgttact attaaggaaa aatcaactat gtttccccac atatacattt
     5041 ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct aataccgagg
     5101 gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa ctgtcgtagc
     5161 ttttttttgc tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt
     5221 ccatatctgt tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc
     5281 gaggtagcca catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa
     5341 gatcatcaga taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc
     5401 aagtcaagga acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa
     5461 caaatcagca aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct
     5521 tagccatttt gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca
     5581 tagccaatac aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac
     5641 taaatgtttt aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag
     5701 atatagaaga ccttgtaata gatgacattg ttattttaga gactggacat caaattccag
     5761 cagatgcaga ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag
     5821 cagatgaaat aaccaaagaa ataggcgatc cattaatgtc cggcagtttc attgtttccg
     5881 gaaaagctta cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag
     5941 tcaaagccaa gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat
     6001 tgatcaaatg gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt
     6061 acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg
     6121 gtatgatacc tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta
     6181 aactagcaaa gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg
     6241 taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa
     6301 aagtgatcgt ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca
     6361 ttgctgacta tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat
     6421 attttacaaa aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa
     6481 aattcagcag tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt
     6541 tgcgcgatca attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc
     6601 gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag
     6661 tcacaccatt aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat
     6721 ttgattactt caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca
     6781 ccgtatccaa tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat
     6841 ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg
     6901 tcaagccaga acaaaagaaa atcttcgttc aattgctaaa gcgtcgtaac aatacagtag
     6961 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     7021 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     7081 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     7141 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     7201 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     7261 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     7321 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     7381 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     7441 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     7501 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     7561 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     7621 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     7681 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagtt aaaaaatttc
     7741 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     7801 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     7861 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     7921 tctacagtaa tcgtaccatg aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     7981 ccggtcccgc ctgaatgccg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     8041 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     8101 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     8161 tcaatcagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     8221 tttccaatca atcgaattgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     8281 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     8341 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     8401 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     8461 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     8521 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     8581 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     8641 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     8701 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     8761 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     8821 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     8881 gtatacgccc gatacatctt actcatttgt tcgctcaacg cattgatcgt ctgatacaat
     8941 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     9001 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     9061 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     9121 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     9181 tggccacttt ttttgactgg taatgcgaca tataaaagat ccccattcaa agtagtactt
     9241 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     9301 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     9361 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     9421 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     9481 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     9541 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     9601 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     9661 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     9721 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     9781 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     9841 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     9901 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     9961 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
    10021 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
    10081 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
    10141 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
    10201 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
    10261 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
    10321 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
    10381 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
    10441 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
    10501 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
    10561 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
    10621 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
    10681 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
    10741 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacgggagt ttttccggaa
    10801 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
    10861 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
    10921 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
    10981 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
    11041 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
    11101 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
    11161 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
    11221 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
    11281 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
    11341 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
    11401 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
    11461 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
    11521 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
    11581 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
    11641 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
    11701 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
    11761 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
    11821 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
    11881 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
    11941 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
    12001 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
    12061 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
    12121 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
    12181 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
    12241 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
    12301 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
    12361 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
    12421 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
    12481 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
    12541 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
    12601 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
    12661 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
    12721 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
    12781 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
    12841 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
    12901 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
    12961 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
    13021 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
    13081 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
    13141 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
    13201 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
    13261 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
    13321 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
    13381 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
    13441 aatggctttt tgacactcca acaggtaagc aaaccactta tgaagctttt tatgaaacaa
    13501 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
    13561 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
    13621 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
    13681 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
    13741 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
    13801 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
    13861 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
    13921 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
    13981 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
    14041 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
    14101 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
    14161 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
    14221 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
    14281 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
    14341 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
    14401 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
    14461 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
    14521 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
    14581 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
    14641 aaaaaagaag tctaaaatct tctatttcct aaaaaaaatc gaatttaatc aagctgtaaa
    14701 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
    14761 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
    14821 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
    14881 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
    14941 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
    15001 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
    15061 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
    15121 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    15181 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    15241 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    15301 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    15361 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    15421 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    15481 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    15541 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    15601 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    15661 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    15721 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    15781 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    15841 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    15901 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    15961 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    16021 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    16081 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    16141 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    16201 tgaggatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    16261 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    16321 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    16381 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    16441 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    16501 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtggg attaacagtc
    16561 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    16621 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    16681 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    16741 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    16801 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    16861 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    16921 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    16981 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    17041 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    17101 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    17161 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    17221 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    17281 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    17341 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    17401 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    17461 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    17521 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    17581 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    17641 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    17701 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    17761 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    17821 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    17881 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    17941 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    18001 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    18061 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactac
//
LOCUS       DAPBBG010000055        17789 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPBBG010000055 DAPBBG010000000
VERSION     DAPBBG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.056"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(96..2216)
                     /locus_tag="RD477_002198"
     CDS             complement(96..2216)
                     /locus_tag="RD477_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7634527.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2446..3624
                     /locus_tag="RD477_002199"
     CDS             2446..3624
                     /locus_tag="RD477_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDT7634528.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3640..4398
                     /gene="aroD"
                     /locus_tag="RD477_002200"
     CDS             3640..4398
                     /gene="aroD"
                     /locus_tag="RD477_002200"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDT7634529.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4421..4906
                     /gene="ybaK"
                     /locus_tag="RD477_002201"
     CDS             4421..4906
                     /gene="ybaK"
                     /locus_tag="RD477_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDT7634530.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4977..6230)
                     /locus_tag="RD477_002202"
     CDS             complement(4977..6230)
                     /locus_tag="RD477_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7634531.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6347..7696
                     /gene="gdhA"
                     /locus_tag="RD477_002203"
     CDS             6347..7696
                     /gene="gdhA"
                     /locus_tag="RD477_002203"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDT7634532.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7808..9154
                     /locus_tag="RD477_002204"
     CDS             7808..9154
                     /locus_tag="RD477_002204"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDT7634533.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9462..9848)
                     /locus_tag="RD477_002205"
     CDS             complement(9462..9848)
                     /locus_tag="RD477_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7634534.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10116..10265)
                     /locus_tag="RD477_002206"
     CDS             complement(10116..10265)
                     /locus_tag="RD477_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634535.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10727..10939
                     /locus_tag="RD477_002207"
     CDS             10727..10939
                     /locus_tag="RD477_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634536.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11217..13016)
                     /locus_tag="RD477_002208"
     CDS             complement(11217..13016)
                     /locus_tag="RD477_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDT7634537.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(13140..13736)
                     /locus_tag="RD477_002209"
     CDS             complement(13140..13736)
                     /locus_tag="RD477_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7634538.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            14086..14430
                     /locus_tag="RD477_002210"
     CDS             14086..14430
                     /locus_tag="RD477_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDT7634539.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            14431..14850
                     /locus_tag="RD477_002211"
     CDS             14431..14850
                     /locus_tag="RD477_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDT7634540.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(14942..15292)
                     /locus_tag="RD477_002212"
     CDS             complement(14942..15292)
                     /locus_tag="RD477_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634541.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(15258..16589)
                     /locus_tag="RD477_002213"
     CDS             complement(15258..16589)
                     /locus_tag="RD477_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDT7634542.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            16844..17410
                     /locus_tag="RD477_002214"
     CDS             16844..17410
                     /locus_tag="RD477_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7634543.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            17555..17642
                     /locus_tag="RD477_002215"
     tRNA            17555..17642
                     /locus_tag="RD477_002215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17587..17589,aa:Ser,seq:cga)
BASE COUNT         6028 a         3170 c         3459 g         5132 t
ORIGIN      
        1 ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg agcagcgaca
       61 ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact tgtggtcgtt
      121 ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg tcccttgtgt
      181 tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt taatttctct
      241 aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact attcgtatag
      301 aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt atcaactttt
      361 tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat agcttcacca
      421 gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac aaaatcacca
      481 tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc taactgaata
      541 taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc aacttgtccg
      601 ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat ataagggaca
      661 cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc aactaattca
      721 gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag gacgatgact
      781 gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac ggcggtatct
      841 aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt ggctaacggg
      901 aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac agcaatgaat
      961 tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg gaaaaatggt
     1021 ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata agaagaatca
     1081 aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa tgtaccagcg
     1141 ttcccatgga ataccgcact agtgtaacct tgtgtttgac ttaaaatatc aggtgcagct
     1201 tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa gccaaacaat
     1261 gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg atggaaaaag
     1321 ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg tgtaacttca
     1381 agctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg gaagctttct
     1441 aaatggatat aaatcacatt ttttccttta gcgagcccga acaaatcatc atttggtgct
     1501 gcataatgtt cttttacata attttctact tctttcatat cattgggact tgcttctgcc
     1561 cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc attcaagcca
     1621 agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg acgatcagct
     1681 tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat cgcaaaagcc
     1741 attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag cagaacagtc
     1801 aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg aatggcactt
     1861 tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc
     1921 gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag aaaataaatg
     1981 accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat tccaagtaat
     2041 aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc actttcgatt
     2101 ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa aacaactagt
     2161 aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca tttcaagata
     2221 atcttccttt ctgcacttct taatcacaga agtgtcatga aaccatgtta cacagctcac
     2281 ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca gaagtttatg
     2341 ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga caagtcctat
     2401 gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa agtaaaagta
     2461 aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa agaagacctt
     2521 gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca aaaccaattt
     2581 atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt gagccagcag
     2641 aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga
     2701 actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg agaaggagac
     2761 gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc ttggtataac
     2821 gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat ctacccggaa
     2881 atcgtaggag gctatgagaa aattcgattt gaatcagtaa atctgcctga gagccagttc
     2941 atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc
     3001 gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga agtacgggga
     3061 tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag ttataccggc
     3121 gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt cgatctagca
     3181 aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga tgtcgatcag
     3241 caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag aaaacaattt
     3301 ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa aaaagtcttt
     3361 cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt agcagaagac
     3421 gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg
     3481 attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt atatcgtctg
     3541 ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa agtgtttgtt
     3601 tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggtttgggt aaagggattg
     3661 aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt ccgagaggaa
     3721 gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga atggcgagtc
     3781 gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt tcttatccat
     3841 gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga aggcggagaa
     3901 caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca cacaggatta
     3961 gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag actgatagag
     4021 gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa agaaacacca
     4081 gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc cgatatcgga
     4141 aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga gttgactaga
     4201 cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa tcttggtaaa
     4261 atttctcgtc tggctggcgg aacgacgggt tccgttatga catttggttc cttaagcagc
     4321 gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat tattcaatta
     4381 ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga aaaaaatagt
     4441 aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag agcacgaata
     4501 tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc cagaaagcca
     4561 atctcatata tttaaaacac tggttgctgt gggcaatgtg acggggcctc ttgttgctgt
     4621 gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag ggaataaaaa
     4681 agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac gtggtggctg
     4741 ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg cagaaagcta
     4801 tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag cacctcaaga
     4861 tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca tacgtataaa
     4921 agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt tatacattat
     4981 gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc tgtgatatta
     5041 aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag accatcaatg
     5101 attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc gccagttaca
     5161 aatgatttct caataggaaa agttttgttg attgatagct taaactcttt gcatagttga
     5221 acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc catttcaagc
     5281 cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc attgaccaag
     5341 ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg tttggtttgt
     5401 gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa ttctttattg
     5461 acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa
     5521 tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa agtgatgtct
     5581 tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga tatcaagggg
     5641 gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat cttgtaccca
     5701 tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat aatggcagga
     5761 gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat
     5821 ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg attgagcaat
     5881 gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg atcctcttct
     5941 tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt ccattgcgca
     6001 aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc atcaacagga
     6061 cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt tcctgctttt
     6121 ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc
     6181 atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat tccctcacct
     6241 caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga
     6301 gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga caaacgcaac
     6361 agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc aagaagaatt
     6421 tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca ctcatcccga
     6481 atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta ttcaatttcg
     6541 agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc gtatccaata
     6601 caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg tcaatctaag
     6661 tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg gtctgccaat
     6721 cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata atgagatcat
     6781 gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat cgattgatgt
     6841 tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg gcgaatataa
     6901 acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt tttggggaag
     6961 taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac atctattgaa
     7021 cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc
     7081 catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat gttcagattc
     7141 tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga
     7201 aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta tttatcatgc
     7261 tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat gtgctactca
     7321 aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca aaatcgtagc
     7381 agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg aagcaggcgt
     7441 gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg ctttagaaat
     7501 gagtcagaac gcccagcgat tgacttggga aagagagcaa gtggatgagc agctggatca
     7561 aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa
     7621 aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag caatggcagc
     7681 agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt ttgtttattt
     7741 gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc tataaggagg
     7801 agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg tcaacgaaca
     7861 agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac gggaaggtac
     7921 tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg acaaagaaga
     7981 atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag tattagttgt
     8041 tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct tgaatcacac
     8101 attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct ttgccggaaa
     8161 ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc gcgacttttc
     8221 agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt tccgtgtatt
     8281 caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta tctacgctac
     8341 aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt gggaaacttt
     8401 cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg gtttgttacc
     8461 aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa
     8521 agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag caatgcgcaa
     8581 cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac caggaatgca
     8641 atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag accaaaaagg
     8701 catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac aattcatcca
     8761 agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat
     8821 cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag gaaaagaagt
     8881 ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag acggtgacgt
     8941 gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt atacaatgta
     9001 cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc cattcgacca
     9061 accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac cgggctttga
     9121 agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc tagatttaaa
     9181 taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt taggtactca
     9241 aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt cacagtccct
     9301 tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga ctttaagaac
     9361 agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca gtaagcaaaa
     9421 aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag gcctaatttt
     9481 tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat gatccgcttt
     9541 tcgcttgctt tcaactgcaa gatcgttcct tcggtgtaag cagaaccatt ggcagagtca
     9601 ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc ataagcatct
     9661 acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat atcagtttct
     9721 gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt agactgatag
     9781 tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa ccctaaaatt
     9841 tttttcatga aagatctcct tttttatttt tattatatta aatattatac agataataac
     9901 ggaatattat catcgaaaca gctaacaaaa ttgtcataaa aaacgagatg gttgatatta
     9961 gaacttgtgt ttttgtttag gtctagagat aaagagagac aatttccaaa tatatgtaat
    10021 gaatacaaaa agacgtttgt cgcggattac cgttacaaac gtcttttatt gattttccga
    10081 gatctcatca gtcgccattt ggaatggttc actccttatc tagattcgtt taatgctcgg
    10141 actgcttgtt cgattgtaat accaaacgca acgttatttt cgttttgtgc atcaatcgcc
    10201 ataaaacggt tagaagttgt atcaaaagct acgcggtatt gcatatttgt ttttgtaaac
    10261 gtcataaaat tcatctcctt tgtaatgtaa tttagttctt gaaagtttcc tttaaacttt
    10321 cgaacttata ccaagtttag catcgtacgt tttttgatgc aattaaaaag catttatcat
    10381 tttccttatt tattttttct taaatggttt agctattttc ttttaatccg ataagtagcc
    10441 caatcatcaa ggttacaaat actattgctt gaatataaat catatgctca cctctttata
    10501 aataacttag caatataaag aaaaaaacgc acgaacaaca taggaatata aacaaacgtt
    10561 tttgtcacaa cgtctaagat cgtgttaaaa aatagttatc taaaaagcag aaagatactc
    10621 aaaataaaaa ataaaaaaat gaataaaaac gttttcaaat acaaggtaat ttgttattat
    10681 aaaataaaga aaaaacaaaa taaaaataaa gaaaagaggg aatgaaatgg taaaagcaga
    10741 agtaggaaga tcttatatat gcaaaccgat cggagtatct catgaactaa tcggctgtat
    10801 tgaacgtaca tatatcaaca cggctgtcgt gtatgttgaa agttatgacc aacgagacta
    10861 tcaacttatc aatgaaagca agtatcgcat gctcgtaaaa ttctgtgata ttagcgcacc
    10921 tactgaattg gtttcttaaa cagtattttt gagaattcgc aagaactccc ttataatata
    10981 aataaagaca gaagggagcg agaagatatg ccaaaaagaa aaagttcagg gaaaatggga
    11041 attattcttg cagtgatctc tgcaattcct gctattttag ctattgttcg tgcgatgaag
    11101 gaatcaagag aaagttaagc acacattatg agcgtcaaac aaaaaacgca tatacctgcg
    11161 gtggcaggtt atgcgttttt tgttgtataa agactttttt cattaacgat ttttgattag
    11221 ccgactccca aaacaaaatt tagcagttta tctgaataag tgatttcttc atctgtttgt
    11281 ttcacacttt catttagtag tcccatctca tcggttatga taccatccac accataaaac
    11341 atcatgcgac tcatggcatc tgaatcgttg attgtccata catatacttc ttttccgtct
    11401 tggtgggcag aattaatgaa gttacggttg atcgtactat attccattgt cagaaaatca
    11461 gcaggcgtca cagggggccc aacaatattg aaaggtaaga tgtagcccac ataaaagttt
    11521 ggttctgctt cttttaaatc tgaaacggtt tggtacgtca aactttgcaa aatatgatgg
    11581 ttctctaaga taactttgcg gtatttatgg acaaatcgtt cgacaaggtc atccgaatct
    11641 tttttagagg tcttgatctc aataagtaat ttttggttta gcttatgtgc tttttccaaa
    11701 taagaatcaa aagaacaaac aggggcttgc actccgtttt cagtaactgt aagatttgtc
    11761 aattctttca atgtaagatc ctgtggtgtt ccatttaccc ctgttaagtg acgaagattt
    11821 gcatcatgca tcactacgaa atgaccatct tttgtttcct gaacatccat ctctacataa
    11881 tcaggatgat agtctctgct tgttttttca agtgcttcta aagtattttg cgccccattt
    11941 tgggtactca cacctctgtg agagacagtc actggttttg tctgaacagc tgaagtcaga
    12001 taatccatat tgtaaagaca gacgccaata ccgaaaaaaa ctgcaaaaag agtcaaacca
    12061 gtgtttttca gcgcagagaa acgcaagttg ggggcttgtg ggataaacca cttagggatc
    12121 tcaggcaaga agccttcgtc atccatgaaa tcaacaatga tataaaaaat ccctacggta
    12181 gacatcacga tattgaataa taaaatccct tgtaaaagcg tcatagcaaa cacagcgctg
    12241 atcaatgcat agtctgggaa aaattgttca accatacttt gtgccaaaat aacaacgata
    12301 tatcctaaag aagaaaggag taaaatcgta ccgccaatca caataaattg gcccgtgatg
    12361 gccaaaagac gcgatttggt caatgaccag ctttcccgaa tggctgctcg aaaagaacga
    12421 tctcgtagaa tcatttccgg taaagcaaaa atcagtcgaa tcccaatata gccaaggaaa
    12481 atataaacga gtaaaaagga actgacgatg atccaacgat tagcaaaaat aaaatccata
    12541 atgaaagcgg ggatttttat ttttgacagt agatcagaat taaaacttaa tccgctgatc
    12601 ggtagaatca ataaaaaata agcaaggaaa aacaaaaaag taatgggtct cactttcttc
    12661 agctgaagta tcgtcaagtg taagagttgc ttcaaagaga ttggttcttt ctttttaata
    12721 aaaaaaacac tcatcaaaag aaaagtaaat tcgaaataaa ctaacagtaa aatcgtcatc
    12781 aaaactacga ttaacgagag tagaacccca ggatgttgag aaaaaattga aggaatattg
    12841 tcataagaca aatagtcaat tgcccctcgg tgtaaaataa aacgggtcgt actgctcaat
    12901 aagggtaaac aaataaataa aataaagcca tgcatcagca agacatcacg gaaataagct
    12961 tgtgttcctt ttaaaaagtc ccaagcatta cctaagctac gtttcaaata attcatccta
    13021 tcacctgctc ttcacattgt agcgcaaatc taatgaaaaa ggaaagggtg cagtgtgttt
    13081 tgcagaccct ttcccagatt taacattgtc aatcaatgac taattttaca aaataaatat
    13141 tatccaagaa gaccacttag atattgacct ccataaatca aggccatact atataagaaa
    13201 atcgacagtg gtttagcaat caaaatgatt agtgtatatc gtttcactga catgtttgtc
    13261 aatccagcca tcaaacataa cgcatcgtca ggagcaactg gaagaaaaat agctagagca
    13321 aacagcttct caaaacggtt ttgattatcc agccagccaa tatatttgtt atatgtctta
    13381 tcgctaatca tggaaagtat gaatggcttt ccataacgac gccctagcag aaaaatgata
    13441 atcgaaccaa tgcagattcc aatatagtta tagataaatc cagcataagg gccaaatatc
    13501 aggacaccag ctgcagtact gatccctcca ggaatgattg gaataactac ttgcaggatt
    13561 tggatgaaga caaaaataat cggtcctaaa atgatggaat cacctactaa tcctcttaac
    13621 gcattcaaat ctttgaaaac acctaaatta ataaaataaa tcgtcagcgc gattgatagg
    13681 cctaatccga taatggatat gaaatttatc agttttcgag aggcagcaat attcattatt
    13741 catcaactcc ttagaggttc tttgttaact atactttact agaaaagcaa ctaactgcgc
    13801 cattttttga tatgacagaa aacaaacaat tttgtaagtt catcataagt ataaaagatt
    13861 gatctcaaaa aaacatccaa ctaaagactt atttaataac ttgttttttt taaaataaaa
    13921 aaagcgttag caactatgct taaaaagcat atcgactgaa taaactaagc attggacagg
    13981 ctgaaagact acatttaaaa tgaaatgcga agaaacaata gaaaataaag ttgccttaga
    14041 accatagttg aatcacctaa cagtcgtgaa gggagaataa gagaaatggc agaaaaacaa
    14101 accgcaggac gagatcaatt aggagatttt gcaccaaaat ttgcagaatt aaatgatgat
    14161 gttttatttg gacaagtatg gtccagagaa gaattatctg ctcatcagcg tagtatcatc
    14221 accatatctg cattaatttc aggaggaaat tttgaacaat tgccagctca tctgaagatt
    14281 ggcaagcaaa atggcattac tagtgaagaa atagcagaaa tcattaccca tttagctttt
    14341 tatgtagaat ggccaaaagc ttggtcagct tttagtttag caaaagaaat ctatttagaa
    14401 gaaaatagcg aagataagaa agaggcgtaa atgatgacaa aacacgaaga aatacacaat
    14461 ggcgtgattt ttccagcagg tgaaaaaaac gaagcttacg ctcaagtatt taccgggcaa
    14521 agctatctga atatgttagt gaatgatccc gaagtttctg ttggtgtagg taatgtaact
    14581 tttgaaccag gttgtcgcaa tcactggcat gtccatacag acggctatca aatcttactt
    14641 gtgaccggag gagaaggctg gtatcaggaa gaaggaaagc cagcaagacg attaatggct
    14701 ggagatgtcg tcgtaacgca caaagggatt aaacattggc atggtgcgac aaaagacagt
    14761 tggtttgaac atttggcgat cactgctggt aattcagagt ttttagaacc tgtaacagat
    14821 gaagtgtatg aaggattgga aaatcaataa tgtcaagctt gaacagctcc tgaaagtaga
    14881 ttttaatcga actttcagga gctgcttttt tgattcagtc ttttataaat agaaaatagg
    14941 gttaataata ggaatgagaa gcacgtcgaa agatcttaat attgataatc ggctgggcat
    15001 acatgatttc cacgtcatca atcaaactac ctaacagatg ataatcttcc cgatcatgtc
    15061 ggggattacc taatagttct tgatagtaga tttccatact agggtcccga ggattgttca
    15121 gcaaatcagc aagtacatct aaatcttctg gctgagtttc tgccgtaccc tgtatctcta
    15181 tgaaaagccc ttcctcagca aattctactc caatacgtat ttcagttatt tcatgggaat
    15241 ataaataagt aagaagctca tcgatgattc tttttgataa ttctttttcc aagtgtcttt
    15301 ttttcctttc tgacagcggt aaagttatct agtatcggta caaggatacc tgcaacgaaa
    15361 ccagttgaaa aaccactgtt atatagattt agtcctccgt gcaaagtgct gacgttatgg
    15421 acaagagcaa tatgtagcac accagctatt acaccgtaaa aactgccgta aaaaccgctt
    15481 atgggcgcaa gacctgtccc aaacacagct gcaacaagca tgccagtact ggtgatctca
    15541 tgaaaagttc caaacaaaga agcaataaat attccgacta gtatcggaaa actgtttttt
    15601 aaatgacaac caaaagcact gaaacccacc gcagataaaa tagcaccgat gacaggtcca
    15661 tttaactgac cgccaaccag caaaacataa gaagacagca tcagaccaac taagcccatg
    15721 ttgatcaaag tagcaccaat accagaatta gcaatgaaat cagttgtcaa ttttccagat
    15781 gaatcgaaaa tttctcgaat ccccgaaagc cgaaaggaat tataatagaa accaattccg
    15841 actgtcagcg aaaaaaagcc aaaaataatc caaatgagag gaaagtgata ttcattgaaa
    15901 accaatgtat tttcgacgat tgaatagctg aataacctca aaacagcagt aaaagtcatc
    15961 gcaatcaacc cagaggtgaa tccaatatta taaagtgtga atccacgatg gaaaactaaa
    16021 gtttgagcag ctaagggcgg aagaagtata ccaattgcta tgccagctaa attccccaat
    16081 aaaataccta tcagtaaagg aaaacgcata ccaaaagtga tataactaac gaccggtgat
    16141 aatacgctac caaacagtcc gatcatgatg tactgagaaa aaggtttatt gacaaataca
    16201 gcgtatagat acacacctaa aatgattgag atagaattat aaaggttttt gccaaaaaaa
    16261 gagaaaccgg taactgttag aatcgaagca aataagggac cgtttaaaat caccttgtgc
    16321 cgataagcta aaagcacaga aagtaaggtc aaaatcccta catttatgaa tgcgcttccg
    16381 aaaacaccta aagcaaaata atctgtcaaa agattgcttg gcgaggtaag aatagttagc
    16441 aatccactcc aattatcagt aggagtatta gaaagcaagc caataaaaag aaaaaaaaca
    16501 cctgtaaaac caagataaaa atactttctt ttctgtgaaa cgattcgttt ttgaggatga
    16561 atataaataa cgtcagtttc gttttccatt acacaccttc tttttcattt tctttaacta
    16621 taacttgaaa tgaaagattt ggacaactat catttaggtc aaaactaaaa atgtaaggga
    16681 acccattgaa tttaaccaaa tttttttaat tttgcaaaaa aaagttaaag aagactgaaa
    16741 aaaatttaaa atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa aatagggtac
    16801 aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta
    16861 taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg
    16921 atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga tttatctgga
    16981 attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg aacaactaaa
    17041 agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg gtttgaacta
    17101 tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg atgaacatgg
    17161 ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag ttaaagaaga
    17221 tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg gtacatgggt
    17281 agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa cagaagaaca
    17341 aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata atcctgaaat
    17401 aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct gcaaactgtc
    17461 aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa ataggcttga
    17521 catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt ggtaatgcac
    17581 cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc
    17641 cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc
    17701 aaacaaaaat agcttttcct attttttcgt ttggttgggc ttattaccgc agatgcaagc
    17761 ttttgaacac cgtttgttcg gaataaggg
//
LOCUS       DAPBBG010000056        17231 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPBBG010000056 DAPBBG010000000
VERSION     DAPBBG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.057"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..370
                     /locus_tag="RD477_002216"
     CDS             2..370
                     /locus_tag="RD477_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDT7634544.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="RD477_002217"
     CDS             551..1492
                     /locus_tag="RD477_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDT7634545.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMEAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALSK"
     gene            1561..2292
                     /locus_tag="RD477_002218"
     CDS             1561..2292
                     /locus_tag="RD477_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDT7634546.1"
                     /translation="MKSLLYAIGDVHGKYELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLLHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="RD477_002219"
     CDS             2436..4616
                     /locus_tag="RD477_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDT7634547.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKKVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFLQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLTDILAGLT
                     QPGRDMRVEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="RD477_002220"
     CDS             4636..5118
                     /locus_tag="RD477_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDT7634548.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="RD477_002221"
     CDS             5134..5895
                     /locus_tag="RD477_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDT7634549.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVLEKILLMNDPTLQRRTQATFEKVW
                     KSEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="RD477_002222"
     CDS             6026..7360
                     /locus_tag="RD477_002222"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDT7634550.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     DVPIYYYGVTENDDIQARKIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMMTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="RD477_002223"
     CDS             7666..8151
                     /locus_tag="RD477_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDT7634551.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="RD477_002224"
     CDS             8144..8827
                     /locus_tag="RD477_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDT7634552.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     AVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="RD477_002225"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="RD477_002225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDT7634553.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="RD477_002226"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="RD477_002226"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDT7634554.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="RD477_002227"
     CDS             12443..13072
                     /locus_tag="RD477_002227"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDT7634555.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="RD477_002228"
     CDS             13124..13456
                     /locus_tag="RD477_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDT7634556.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="RD477_002229"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="RD477_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDT7634557.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="RD477_002230"
     CDS             14282..15655
                     /locus_tag="RD477_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDT7634558.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="RD477_002231"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="RD477_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDT7634559.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="RD477_002232"
     CDS             16710..17150
                     /locus_tag="RD477_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDT7634560.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3006 c         3802 g         4548 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcagaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgaag atagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaataa aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacaactgc
      721 ctatatggaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcttatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaagtaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatctat
     1561 atgaaatcat tactttatgc tattggggat gtccatggca aatatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgcttcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttgtatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gagctttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgca tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaaaaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ccttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggtgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttaaccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtgt tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa tgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aagcatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatgggga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatggaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt actagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc aagcaacttt tgagaaagta tggaaaagtg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgactccatg acgatgagaa cggctatggt cccaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttcccgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaactat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcag atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaagatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag tggcagaaga
     6901 aatgatgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgatcg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa cctcatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagatcgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agctcccttt tttgcaactt
     7441 atgataaacg gcgttcaaaa tcttgcatct gcggtaataa gtccagccaa atcaaaaaca
     7501 tgagaagctg tttttcttgg ctgttcttat tacttgatgc agggcagctt tttgacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt gtccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgcagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gatcacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagataa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atgggaattt t
//
LOCUS       DAPBBG010000057        17005 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPBBG010000057 DAPBBG010000000
VERSION     DAPBBG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.058"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..124)
                     /locus_tag="RD477_002233"
     CDS             complement(<1..124)
                     /locus_tag="RD477_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDT7634561.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="RD477_002234"
     CDS             complement(228..539)
                     /locus_tag="RD477_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7634562.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="RD477_002235"
     CDS             complement(541..738)
                     /locus_tag="RD477_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDT7634563.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="RD477_002236"
     CDS             complement(1132..2859)
                     /locus_tag="RD477_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDT7634564.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="RD477_002237"
     CDS             complement(2852..4045)
                     /locus_tag="RD477_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7634565.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="RD477_002238"
     CDS             complement(4232..4876)
                     /locus_tag="RD477_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7634566.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="RD477_002239"
     CDS             complement(4970..5104)
                     /locus_tag="RD477_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDT7634567.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="RD477_002240"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="RD477_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDT7634568.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="RD477_002241"
     CDS             complement(7235..7708)
                     /locus_tag="RD477_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDT7634569.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="RD477_002242"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="RD477_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDT7634570.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="RD477_002243"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="RD477_002243"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDT7634571.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="RD477_002244"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="RD477_002244"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDT7634572.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="RD477_002245"
     CDS             complement(11755..12894)
                     /locus_tag="RD477_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7634573.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="RD477_002246"
     CDS             complement(13203..13916)
                     /locus_tag="RD477_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDT7634574.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="RD477_002247"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="RD477_002247"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDT7634575.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="RD477_002248"
     CDS             15105..16637
                     /locus_tag="RD477_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7634576.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="RD477_002249"
     CDS             complement(16828..>17005)
                     /locus_tag="RD477_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634577.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DAPBBG010000058        16615 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPBBG010000058 DAPBBG010000000
VERSION     DAPBBG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.059"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="RD477_002250"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="RD477_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDT7634578.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="RD477_002251"
     CDS             complement(680..1666)
                     /locus_tag="RD477_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDT7634579.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="RD477_002252"
     CDS             complement(1689..2255)
                     /locus_tag="RD477_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDT7634580.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="RD477_002253"
     CDS             complement(2269..2685)
                     /locus_tag="RD477_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDT7634581.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="RD477_002254"
     CDS             complement(3142..5076)
                     /locus_tag="RD477_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7634582.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5357..6313
                     /locus_tag="RD477_002255"
     CDS             5357..6313
                     /locus_tag="RD477_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDT7634583.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="RD477_002256"
     CDS             6403..7506
                     /locus_tag="RD477_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDT7634584.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="RD477_002257"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="RD477_002257"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDT7634585.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="RD477_002258"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="RD477_002258"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDT7634586.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12140)
                     /locus_tag="RD477_002259"
     CDS             complement(10887..12140)
                     /locus_tag="RD477_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDT7634587.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="RD477_002260"
     CDS             complement(12146..13069)
                     /locus_tag="RD477_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDT7634588.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="RD477_002261"
     CDS             complement(13290..15782)
                     /locus_tag="RD477_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDT7634589.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="RD477_002262"
     CDS             complement(15789..16256)
                     /locus_tag="RD477_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDT7634590.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="RD477_002263"
     tRNA            complement(16392..16465)
                     /locus_tag="RD477_002263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="RD477_002264"
     tRNA            complement(16523..16596)
                     /locus_tag="RD477_002264"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4907 a         3475 c         2958 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg atggcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttga tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DAPBBG010000059        16182 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPBBG010000059 DAPBBG010000000
VERSION     DAPBBG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.062"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..783
                     /locus_tag="RD477_002265"
     CDS             <1..783
                     /locus_tag="RD477_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7634591.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="RD477_002266"
     CDS             1161..3203
                     /locus_tag="RD477_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7634592.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="RD477_002267"
     CDS             3214..3834
                     /locus_tag="RD477_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDT7634593.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="RD477_002268"
     CDS             3846..4304
                     /locus_tag="RD477_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7634594.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="RD477_002269"
     CDS             4323..4601
                     /locus_tag="RD477_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7634595.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="RD477_002270"
     CDS             4626..5993
                     /locus_tag="RD477_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDT7634596.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="RD477_002271"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="RD477_002271"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDT7634597.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="RD477_002272"
     CDS             7180..7671
                     /locus_tag="RD477_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634598.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="RD477_002273"
     CDS             8114..9313
                     /locus_tag="RD477_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634599.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="RD477_002274"
     CDS             complement(9808..10566)
                     /locus_tag="RD477_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7634600.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="RD477_002275"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="RD477_002275"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDT7634601.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="RD477_002276"
     CDS             11666..13111
                     /locus_tag="RD477_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7634602.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="RD477_002277"
     CDS             13139..13594
                     /locus_tag="RD477_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7634603.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="RD477_002278"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="RD477_002278"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDT7634604.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="RD477_002279"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="RD477_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="RD477_002280"
     CDS             15403..16017
                     /locus_tag="RD477_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7634605.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         5059 a         2681 c         3439 g         5003 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatagatt tctgtatcca acaataaaaa tatttttgta tt
//
LOCUS       DAPBBG010000060        16178 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPBBG010000060 DAPBBG010000000
VERSION     DAPBBG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.063"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            7..849
                     /locus_tag="RD477_002281"
     CDS             7..849
                     /locus_tag="RD477_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7634606.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            849..1796
                     /locus_tag="RD477_002282"
     CDS             849..1796
                     /locus_tag="RD477_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDT7634607.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1811..2044
                     /locus_tag="RD477_002283"
     CDS             1811..2044
                     /locus_tag="RD477_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDT7634608.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2375..2959
                     /locus_tag="RD477_002284"
     CDS             2375..2959
                     /locus_tag="RD477_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7634609.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3090..3758
                     /locus_tag="RD477_002285"
     CDS             3090..3758
                     /locus_tag="RD477_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDT7634610.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3769..5184
                     /locus_tag="RD477_002286"
     CDS             3769..5184
                     /locus_tag="RD477_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7634611.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            5202..5771
                     /locus_tag="RD477_002287"
     CDS             5202..5771
                     /locus_tag="RD477_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDT7634612.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5881..7635
                     /locus_tag="RD477_002288"
     CDS             5881..7635
                     /locus_tag="RD477_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634613.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7595..9361
                     /locus_tag="RD477_002289"
     CDS             7595..9361
                     /locus_tag="RD477_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634614.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            9884..10153
                     /gene="rpsN"
                     /locus_tag="RD477_002290"
     CDS             9884..10153
                     /gene="rpsN"
                     /locus_tag="RD477_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDT7634615.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10168..10347
                     /gene="rpmF"
                     /locus_tag="RD477_002291"
     CDS             10168..10347
                     /gene="rpmF"
                     /locus_tag="RD477_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDT7634616.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10379..10756
                     /locus_tag="RD477_002292"
     CDS             10379..10756
                     /locus_tag="RD477_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634617.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10777..10926
                     /gene="rpmG"
                     /locus_tag="RD477_002293"
     CDS             10777..10926
                     /gene="rpmG"
                     /locus_tag="RD477_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7634618.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10950..11933
                     /locus_tag="RD477_002294"
     CDS             10950..11933
                     /locus_tag="RD477_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7634619.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11963..12085
                     /locus_tag="RD477_002295"
     CDS             11963..12085
                     /locus_tag="RD477_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDT7634620.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12103..13626
                     /locus_tag="RD477_002296"
     CDS             12103..13626
                     /locus_tag="RD477_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDT7634621.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13650..13889
                     /locus_tag="RD477_002297"
     CDS             13650..13889
                     /locus_tag="RD477_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634622.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14082..14543)
                     /locus_tag="RD477_002298"
     CDS             complement(14082..14543)
                     /locus_tag="RD477_002298"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7634623.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14698..15885)
                     /locus_tag="RD477_002299"
     CDS             complement(14698..15885)
                     /locus_tag="RD477_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDT7634624.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         5629 a         2330 c         3041 g         5178 t
ORIGIN      
        1 aataatatgc ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct
       61 ctaattacat ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta
      121 agaggaatgt ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt
      181 tcttatattt taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct
      241 atcattatta cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt
      301 actttcagtt cttttttagc attaggagtt atccttatag gagttgcaaa tagctcaacg
      361 gatttatttc atatcttatt tggaaatatt ttagctgtac aagatattga taagtggatt
      421 acaattggcg tttctgtttt agtgttggtt acagttgttc tattttttaa agaattatta
      481 atcacttctt ttgatccatt aatggcaaaa gcaatgggga tgaatgtcag tttttatcat
      541 tatctattaa tgattctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact
      601 atattaattg ttgcaatgtt aattacacca gctgctacag catatttata tgcaaacagc
      661 ttaaaaacta tgatatttat ttcggcgaca gtcggagcgt gctcatcatt gctaggtttg
      721 ttcattggtt atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc
      781 ttatttgtaa ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga
      841 aagttatgat gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt
      901 ttatattcgg ggcatgttca acaaatagta aagacaaaga tacagtggct tcgaacgaaa
      961 aattaaaggt agtagttact aattcgattt tagcagatat tactgaaaat atagcaaaag
     1021 ataaaattga tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt
     1081 tgcctgaaga tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact
     1141 tggaaactgg aggaaatgct tggtttacaa aattagtaaa aaatgcgaac aaagaggaaa
     1201 acaaagacta ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg
     1261 agaaagggaa ggaagatccc catgcttggt taaatttaga aaacggtatt atttacgcta
     1321 aaaatattga aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa
     1381 atctagataa gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg
     1441 cttcaattcc gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct
     1501 cgaaagcgta taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa
     1561 caccagatca aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat
     1621 tcgtagaaag cagtgtggac gatagaccaa tgaaaacagt atcaaaagat accaatattc
     1681 ctatctattc aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt
     1741 actatgcgat gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg
     1801 gagggtaggt atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca
     1861 ggaattatca aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt
     1921 tagtccagca tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat
     1981 aggatttcaa ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa
     2041 gtaaaagact ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga
     2101 cttaccctaa tagctggata taattttttt aaatctttct cggattattt tagaaaaggt
     2161 gaatgattta gatgagtcta ttaattcttg taggattgct ttttgtatca gttattttgc
     2221 tctttctcat tagtttagct ctaaggaatg agttcagtaa tagacgttaa cgattgaaac
     2281 ttcaagtcct gtgtaagata atatagttaa ttcatcttat taggaggaag tacccataga
     2341 aaccagagat tatattacaa ctcaccaaaa attaatagaa agtggactaa gccattttga
     2401 aaaagaagga tttcagcaaa ctaagataag gcaactgtgc ttgaccgcag gcgtaactac
     2461 gggtgctttt tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
     2521 gcatattaat gcgcttatta atatgtataa tgaagagttt accaaatacc tcttaaactt
     2581 agaaaatata gatatagaga aaagttggag agagaacttc aatgaattag atccatttat
     2641 taattatatt tttgagaata aacgtatatt cgatttggtt ttattccaat cagctggaac
     2701 caaatatcaa gatattgttg aaaaaattac atcatttgtg acaaaaaaga cagtctgttg
     2761 tctagataag ttaaagacta tcggaatgat caatcaaaat ttagtcttta atgaagaaga
     2821 aatccacttc tacatttact cattctacgc tacattctat gatatattga agcatagcta
     2881 ttcaaaagaa aagactatgc tattaaccag aagattgtat caattttctg ttcctgggtg
     2941 gattgaatta ctgagttaat taaaaaaaag cttgcattga gaattgttct cagttatgct
     3001 ttttgataag atcgcactgt tatctgaata acggtgcatt tttgtcagct taagataaca
     3061 gcgcgatctt attttttgga ggtgaaagag tgtcaaaaat caatccgtta accttgatta
     3121 ctttaaatat tttttttccg gtcattattt ttttaggaaa gggaaatata tttcaagtgg
     3181 ggtgtttcat tattgctaca gttttagtat tgatgtttcg taaaataaag acatgtattt
     3241 atttcatctt gatttattca gttctgtttt tagtaatgaa aattattgat gctatcaaga
     3301 tagagttttt tactatattt tttgggacat ccttatatat tgtatttcga atgttgccgg
     3361 tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta ttatcctctt
     3421 tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg atcagattct
     3481 ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg agaaacattc
     3541 gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc gttcttatac
     3601 gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg atagaatctc
     3661 ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat atcatcctgt
     3721 tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt gtttgaattt
     3781 aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga ccttaatttt
     3841 tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg gaaatccaca
     3901 ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt ggttggggaa
     3961 attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc aaaaaatatt
     4021 ggctacgtaa gtcaaaatcc tagaggacaa ttctttacaa caaatacaac gagtgagtta
     4081 gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag aatcaatgaa
     4141 ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc gctatcaagt
     4201 ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa attaattata
     4261 ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc tgacttactt
     4321 tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt ttactatttg
     4381 aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt
     4441 cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt taatagttcg
     4501 attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga agaaattact
     4561 tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt tattggtttg
     4621 gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa gattattcgc
     4681 cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg
     4741 caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc cctggggaat
     4801 gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact ttataaaatg
     4861 aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt ggtttctata
     4921 gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg gttggattat
     4981 atcaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt
     5041 atagccactc atgatccgga atttctttat aaaacatgca atagaattat ttatttaaaa
     5101 aatggtggaa taaaaaaaga tataaaactt agagtagaag attcaacttt gatagaacag
     5161 atttttaatg acataacgaa atagaattga ggagaatata tatgaaaaca aaagattata
     5221 tttttattgg tttagctaca gttatttccc tagtaattta ctttttttca ataatggtta
     5281 gtagtattgg tggggcgttt ggtcacagca tatccccagg gatttttgga ttattatctg
     5341 gaatcatatt tgtttatatt agctataact accctagaaa aggaattttt actatttaca
     5401 caattgtttt attgttattt tttacgttaa tgggcggggc gtacttacct tggtttatca
     5461 gttcgattag tatggcgatc ttagctgata ttattctatc tgtatttgga tatgatcgag
     5521 caattcctca agttgtgtct tgggttctaa tgcaactagg cagtgctgcc ggacagtgga
     5581 ttcccatatg gttctttaca gatcgttttc gtcaagactg gatagataga ggacaatctg
     5641 ctgcaacaat ggatgcaatg atccactacg cggtgggtat atggggaatt atttcagtgt
     5701 tagttgtagc aagtttatct atgattggtg tattgattgg aagaaaagtc ttaaaaaaat
     5761 acaaaaaata gaacgtagtt taccttctat ctgtttgtac atctctaatt tacgctatta
     5821 cggaaagcat taacagttgt aaacgaataa gagaagaaat tgaatgcggg ggaggaaaaa
     5881 atgaaagtat acaaaaaatt atttagttat gtccctaaaa tgaaatacta cggatggtta
     5941 gccactttat tttctgattt ttctgtcgta ttaacagtct atggatatta cagtatttac
     6001 aaatttcttc agtctctaat tattgtagga gatgaatatt tagctaagag ttatgcggtt
     6061 caaactgttg cttggttaac tttaggagct ttattttata ttttttctgg cttattttct
     6121 catatccttg ggtttcgctt agagactaat ttgagaaaaa agggaattag cggtttaaca
     6181 gaagcaagtt ttcgtttttt tgatttaaat tcttcgggat atattcgaaa gacaattgat
     6241 gacaacgcgg ctaaaacaca tatggcaatt gctcacttga ttccagatag tgctcaagcc
     6301 atagtaacac ctgttttatc agttgcctta ggctttttca ttaactttaa aattggattg
     6361 gttctaatcg ccatgttagt aattggtgta ttcttgttaa agaaaatgac agagaataca
     6421 aattttattc aaaaatatca agaatcgtta gatgtgttaa gctctgaaac ggtagaatat
     6481 atccgcggaa ttcaagttat aaagatattt ggtgctaaag taacatcatt taaagccttg
     6541 aataaagcaa ttaacgatta tgctaagttt gcatataagt attctttgag tgggaaaaca
     6601 ccttttgttc tttttcaatg gctatttttt ggcatagttt gcattttgat agttcctata
     6661 acctttttta taacttctct taccactccc aagatcttag ctgttgactt gttgatgttg
     6721 ttttttttaa gtggaattat ttttgtttct ttcatgagaa taatgtatgt aagtatgcac
     6781 ttgtttgaag caagatatgc agtcgaaaca ctagactccc tttatttaga aatgaagcaa
     6841 gaccaactaa aatatgggaa cgagacacag attaaaaatt gtgacattgc atttgaaaat
     6901 gtcagctttt catacaaaga agagtgtgtt ttagaaaatt tcaacttagt tttagaacaa
     6961 aaaagaattt atgcattagt aggaaaatca ggaagcggaa agagtacgat agctaaacta
     7021 atttcaggtt tctacaatgt ggataaggga cagattaaaa ttggtggtaa gcctttaaat
     7081 cactattcaa aagacacaat tattaacaca attgcttttg tctttcaaga tacgaaacta
     7141 ttcaatcaga gtatttacga taacgtttct atagcaaatc ctcaagcgcc agcaagtaaa
     7201 gtatttgacg cgctaagatt agccggttgc gaatcgattt tagaaaaatt tcccgaaaga
     7261 gagcatacct taattggtag taaaggagtt tatctgtcag gaggagaaaa acaacgtata
     7321 gctattgcca gagcaatttt aaaagatgca aagattgtta ttatggatga agccagtgca
     7381 tctattgatc cagataatga gtatgaacta caaaaggctt ttcaatattt gatgaaagaa
     7441 aaaacagtta ttatgatagc tcatcgatta tccagcatta aatctgtaga tgaaattctt
     7501 gtcttgtcag aaggaaaaat agtagaacgt ggaaatcata atgaattaat ggctaaacat
     7561 cgagtgtata aacaattggt cactaattat gagaatgcaa atgattggag gcttgacaat
     7621 gaaaaatttt tataaaaaaa aatttgcttt aactgatcag ggcgcagaag ctttaactaa
     7681 agcttctatt tctagttttt ttgtatactg cattaatatg gtaccagcat tcattataat
     7741 gatgctgata gacgaattag ttttagaaaa tgcaaagccc cgttggcttt attttgcagt
     7801 atcgtttgtt actttgcttt tcatgtattg gttgttagat agggagtatg aaaacttata
     7861 taatagtact tataaagaaa gtgcgcattt aagagtgcaa attgcagacg atttgtcaaa
     7921 tttaccatta tcctattttt caaaacataa tttatcagat ttatctcaaa ctatcatgtc
     7981 tgacgttgaa ggtattgagc atgcgatgag tcatgcaata cctaaagccg gcggtatggc
     8041 tctgtttttc ccttttattt cagtgatgct tttggttggt aatgtcaaaa tgggattagc
     8101 tgttattttg ccaacgttat ttagttttgt cttaatcttg ttatcaaaga aatcccaaac
     8161 gaaagccaat actaaatatt acgatacttt gagagaaaac tcggaagaat ttcaagaaac
     8221 tattgaattg cagcaagaga ttaatagctt taatctatct aaaaaagttc aagacagact
     8281 tttcaaaaaa atggaagaga gtgaaaggat tcatttaaag gtagaattaa gtactttttc
     8341 agtcatggcc ttatcctcta ttttctcata tgttagttta gcagtagtca ttctagtagg
     8401 tgttcactta ctgttaacgg gtgaagtgac tatactctac gtcgttggtt acttactagc
     8461 cgcaataaaa ataaaggatt ccttcgattc tatgaaagaa gcagtgcttg aaatatttta
     8521 tttagctcct aaaatacagc gaattagagc tatgaaggaa accagtatcc aagagggatc
     8581 agatagtcct ctgaaatctt ttgacattga gttgcgagat gtctctttct catatgataa
     8641 caatacccca attttagatc atatctcttt tacggctaaa caaggagaag tgactgcgtt
     8701 agtaggtgct agtggttccg ggaaaactag tatcttgaaa ttagtgtcta gactgtatga
     8761 ttatgatgaa ggctgtattt taattgatgg gtatgatatt aaacgtgtat ctccagcttc
     8821 tttgtttagc aaaatcgcta ttgtttttca agaggtaact ttgtttaata caagtatttt
     8881 ggaaaatata cgtattggaa atagtcaagc gtcagatgag gaagtgaaaa aagcagctcg
     8941 tttagctaac tgcgaagact ttattgaaaa actacctgac ggttatcata cattggtcgg
     9001 agaaaacgga agttcacttt ctggaggaga gcgacaacgc ctatccatag cacgggcttt
     9061 tttgaaaaat gcgccaattt taattttaga tgaaattact gccagtttag atgcggaaaa
     9121 tgaaaaacgt attcaggaaa gtttaaatcg tttaatacaa gataagacag tactaattat
     9181 atcgcatcgc ttgaagtcta ttgaaaaggt aaataaaatt gtagtgatgg atcaaggaaa
     9241 actagttgat caaggtactc ataatgagtt gtatcgaagg tcagaaatat acaaaaatct
     9301 cataaagaaa actaaactat ctgagaaatt cgtctatgaa aaagaagctc aatcacgatg
     9361 atgaaatagg ctttaaatta acctacaatt tttgggatat agttataatt ctttttcaaa
     9421 aagaataatt ttattaaaca gcatctaatt ttgaatacac gaaagaatca gtatgattga
     9481 attagttcac ttaatgtcta ttcttataat ataaggaata atcaaagttt taaaaaattg
     9541 gcgataaacc agttaaacat aaaagattga ataaaaagtt atagtttctt tcatattgcc
     9601 gttttaagta gaggaaaagc tacttaaaac ggtatttgtg tttgattgga ctaataaaag
     9661 tgaattctta tttttcagag cctaaactgc atattctttt aaatgatttg attatctcta
     9721 tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat
     9781 ttattgttga caagagcaac tcttgttggt aaagtatcag tgtcaaaacg taacgattac
     9841 gatttgaggt gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa
     9901 aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tatgcagata aacgtcagga
     9961 attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa
    10021 tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg
    10081 tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa
    10141 agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg
    10201 aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa
    10261 gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag
    10321 aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat
    10381 ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca
    10441 gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga
    10501 aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga
    10561 ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga
    10621 ggagttattt caatgtatcg atggttattg ggagatacct gttgatatga aagaaaattt
    10681 agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata
    10741 tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa
    10801 tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa
    10861 cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt taaagaagtg
    10921 aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt
    10981 ttttaggggc tggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca
    11041 gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat
    11101 tagttcatag cgaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc
    11161 gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac
    11221 caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa
    11281 cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta
    11341 ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca
    11401 tggcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat
    11461 tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc acacatagtg
    11521 aaacgattca aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg
    11581 aaagtgattc atcagaaaat cactataatg gcaaatacta tcatcaacac catcaagtac
    11641 atgagtttaa atcaattctc ttaacttcag atcaagacat aaaaaaagat tttttactcc
    11701 aatggattga ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga
    11761 ttgctttaca agagcaggat tttatagttg ccatgcaaga ggtaaacgaa caggttaatt
    11821 ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg
    11881 atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag
    11941 tagtgattgg gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact
    12001 taaaagcccg aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg
    12061 aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta
    12121 ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca
    12181 aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat
    12241 gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt tccagcggga
    12301 tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat
    12361 gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg
    12421 aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt
    12481 gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg
    12541 tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa
    12601 ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg
    12661 aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg
    12721 acgcaacacg cagcctttgg atatttagct ttggattatg gattgaaaca agttcctatt
    12781 gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat
    12841 gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaattgca
    12901 aaaactttgg ccaacgaagc tgggataaaa ttggaagtac tgaatcctct tgaaagtctt
    12961 acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa
    13021 gcgttagaaa aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag
    13081 gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg
    13141 agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat
    13201 gaagttttcg attacaaagc aaaaatcaat aaagacatga cagctgccga gtacaaagat
    13261 tattatacta ctggctataa gacagatatt gatactatca acattaaaga caacaccatt
    13321 gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt
    13381 ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt
    13441 gctggtcgtt ttaaatatat tcaatttagt gatcatggaa ttgcaccaag taaggcagag
    13501 catttccata ttttctttgg tggagaaagt caagaaaaac tctataatga attgcataat
    13561 tggcctactt tctatccagc ttcattgtct aaacatgaaa ttgctcaaga aatgatggct
    13621 cattaaaatt caatttttgg aggaaataca tggtaagaaa agtattattg tctatggatt
    13681 ccaagaatat tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga
    13741 gtacattagc tgatattgct gcaactttac atgttagtat tgacacagtt catagagtat
    13801 taaaacggga tcctgattgt tgttttatca aggtcaatac tggaagaagt agacgtttag
    13861 caaaacggca taatagcttg gccaattaat tgataatacc agagtagatc atttttccct
    13921 tatttggatt tgatctgctt tttattatgt cttgaattgt caaatcaagc gcaacacttt
    13981 atcaaagaag acttcataag gtgttctcca atctaaacac tttctaggtc tgttattcag
    14041 tttttcaatc atctgattta tcgttgagtt atctaccgta gctatctctt tcgattttgg
    14101 caagtattct cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc aagggcatga
    14161 ggatctggaa aataaaaggg catattattc aatgcttctg taactgcgaa atgattggag
    14221 aattcttttc ctctatctgg agtgatcgtt ttgaccgcct catttggaat actgcttaaa
    14281 agttcaatca ttttctcgct cacataagcg gctttctttc tggggacctt ctttcctgct
    14341 aaaaggaagc atgattttct gtctgtcaaa gtcactaaac aagtaccacc agttttgcca
    14401 gctacaatat cggctttcca atgaccaaaa tcacttctat catctgcgga ttttggacgg
    14461 ccatggatcg aatgagtaag gcaaatcttt ccacgagact cgacatgatt ttttgtatgt
    14521 cttgtttttc ctcgatggtg caatgaacgg atcaggcctt tattgccgtg tgagagacta
    14581 actatataga gtcaagtaaa aattagccaa atcaaaaaat attttataac agataattcc
    14641 gaaaaaaggg tacaacaaat aagcttctta ccattagttt ttttgaatgt tcaatagcta
    14701 atcagaaaca attatataag gctcgttttt agtcaaaata gcatgaatca cgtaacaaag
    14761 cttacgagaa accgcaccta aggcagttag atgatgtttt ccttcggaaa cttttttctg
    14821 gtaaaacgca ctaaaaacag gatcacattg agaagctact aaagccgccg aaaacaaggc
    14881 ttttcgcaaa tatggagacc ctcgtttact cataacatta tgcgttgcct catattgccc
    14941 tgattgtgtc acggaagcgt ctatcccagc gtaagcaacg agttttttcg ggttagaaaa
    15001 tttttgaata tctccgatct caccaagaat agtcgcaccg tttataggac caattccagg
    15061 aattgtgaga ataacagaat ttaaagtgtc cattatgtgc ttgatttctc tttccgtttc
    15121 ctttatttgg ctttcaatga attttaattg ttcaatcatt gaacgtagtt ggaaggtgaa
    15181 cgcttcttga gcaaatttta ctccaaagga gttagaagct gcttgcttca attggtccgc
    15241 tttttctgcc ttgattcggt tcctgcttaa ctcagctaag tattgggcca aagtttctga
    15301 ggaaatgttt tctaagtcga tcggggcact aaacgccagc aataactctt tagaagcttt
    15361 tccgaaaacg ccctgcttgg taaaaatcgt cgcgtattct ggaaagactt gatccaatac
    15421 ggctataatt tttcttttga agtcgctcgc tgtacctact aaataagttc ggtaacgaga
    15481 aagttgtttc aaagaaaaaa cattttcatc ggataagacc gtttcgacaa atgagccata
    15541 gcgcattaag tcagcaatca aaacagaatc aataatatcg ttttttcgtt tccgtatttc
    15601 tgtaccttta cgccatccgt ctgtttgaag gggattcaca acgtgaatca aataatctga
    15661 ttcgtgaagg tatgaaaaga tggacagcca atagtgcccc gtggcttcca tccctataac
    15721 aaagtcactg ggtgctccgg aatagtcgac cattcgctca attaatgact ctgctccttc
    15781 agtggtgtta ggaaatgaaa aaccttttag taataccttt ccttcctcgt tcatgataga
    15841 agcaacgtga gtgcgtttac cgatatcaat gcctaaaaag tacatgtata tctgaccttt
    15901 caaaaaaaga attttggatt gaaatccact caactgaaaa gcgtcactac cttgtacgag
    15961 atacggagta aagtaaggac tttcaacatc taactcatac gcaatccact tatcagagag
    16021 aggagccagt ctctcgagtg cgggttcaat ggcccaaggg gggaacggct tagcctctca
    16081 atccaataaa tatattatct caaaagaaat gagttaatac caaattaaaa taacgatggt
    16141 tcatagggtt atccaatcaa aaccggaagg ttaaaacc
//
LOCUS       DAPBBG010000061        15155 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPBBG010000061 DAPBBG010000000
VERSION     DAPBBG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.064"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..393
                     /locus_tag="RD477_002300"
     CDS             <1..393
                     /locus_tag="RD477_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDT7634625.1"
                     /translation="YPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLT
                     TAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLF
                     SEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            593..907
                     /locus_tag="RD477_002301"
     CDS             593..907
                     /locus_tag="RD477_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7634626.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            928..1305
                     /locus_tag="RD477_002302"
     CDS             928..1305
                     /locus_tag="RD477_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7634627.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1315..2088
                     /locus_tag="RD477_002303"
     CDS             1315..2088
                     /locus_tag="RD477_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634628.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2110..3513
                     /locus_tag="RD477_002304"
     CDS             2110..3513
                     /locus_tag="RD477_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDT7634629.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3695..4879
                     /locus_tag="RD477_002305"
     CDS             3695..4879
                     /locus_tag="RD477_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7634630.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4876..5166
                     /locus_tag="RD477_002306"
     CDS             4876..5166
                     /locus_tag="RD477_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634631.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5163..5384
                     /locus_tag="RD477_002307"
     CDS             5163..5384
                     /locus_tag="RD477_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634632.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5426..6205
                     /locus_tag="RD477_002308"
     CDS             5426..6205
                     /locus_tag="RD477_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDT7634633.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6272..6766
                     /locus_tag="RD477_002309"
     CDS             6272..6766
                     /locus_tag="RD477_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDT7634634.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6822..7460
                     /locus_tag="RD477_002310"
     CDS             6822..7460
                     /locus_tag="RD477_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634635.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7532..7927
                     /locus_tag="RD477_002311"
     CDS             7532..7927
                     /locus_tag="RD477_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7634636.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7911..10364
                     /locus_tag="RD477_002312"
     CDS             7911..10364
                     /locus_tag="RD477_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7634637.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10361..12388
                     /locus_tag="RD477_002313"
     CDS             10361..12388
                     /locus_tag="RD477_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634638.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12385..13407
                     /locus_tag="RD477_002314"
     CDS             12385..13407
                     /locus_tag="RD477_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDT7634639.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13424..14353
                     /locus_tag="RD477_002315"
     CDS             13424..14353
                     /locus_tag="RD477_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7634640.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14598..14714
                     /locus_tag="RD477_002316"
     CDS             14598..14714
                     /locus_tag="RD477_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDT7634641.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14730..>15155
                     /locus_tag="RD477_002317"
     CDS             14730..>15155
                     /locus_tag="RD477_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7634642.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQ"
BASE COUNT         5141 a         2533 c         3006 g         4475 t
ORIGIN      
        1 tacccgtcta tttttaagca ctatctattt tttattaacg aagtagaaga tcggataaaa
       61 aataattcat tatctagtag tgtatctagt tttaagaaga tgtttaaatc tcgagatggt
      121 atcacactta ctacagcaca taaagttaaa ggaaatgagt atgatactgt tatcgctatt
      181 aatcttctaa acgggaaaat tcctcattgg aatgaaattt ttaatgtttc tgataaagga
      241 attagttcag cgagaaaact actatacgta atttgttcaa gagcaaaaaa taatttattt
      301 cttttctcag aggtgggtta ttttacgagt aaaggagact tgctttatcc cactcaatta
      361 ttagataaaa tagaatttga ttataatatt taaaatacct aggtagtcgt tgttttaaat
      421 atagtaggaa tcggacgctc agtacggagc gtctattttt ttacccaatt ttgaaaggaa
      481 gtgaacttat gaaaataaaa aatcaagaat caaaaggtcg ctctcccccc tttaaaacca
      541 tcacacaaat aagattcatt caaacataaa aacagaaagg ataggtaaaa atatggaact
      601 taaatatgtc attcccaata tggaaaaaac atttggacaa ttagaatatg ctggtgaagg
      661 gaacattgaa caacgtcgaa taaatggcag accaaccatt ctctctcgta gttacaattt
      721 atactcaacg attcaacgag cagatgatat tgttgtcatt cttccctccg aagctggcga
      781 gaaacatttt gaatccgacg aaaaagtcaa actcatcaac ccacatatta ccgcagaagg
      841 ttataaaatt ggcaatcgag gatttaccaa ttacattatg cacgcagacg atatggtgaa
      901 agcataagaa aggaaaggtg aaaaaatatg agattagcag aaggtattgt gattgaaaaa
      961 gaccagacat ttggcacatt gaaattttcc gccttgcgtc gtgaggttcg tatccagaat
     1021 gaagatggta cggtttccga agaaattaaa gaacgtacct atgacttaaa atcaaaagga
     1081 caaggacgca tgatacaagt cagtatcccc gcttctgttc ctttgaaaga atttgactac
     1141 aacgcagaag tggaattaat caatcctgtg gcagataccg tagcgacggc tacttttcga
     1201 ggggcagacg ttgattggta cgtgaaagca gacgatcttg tattaaaagg acagacaaaa
     1261 cctcatcaga aatataaaaa agaagcgtcc gaaagtgaaa aataggtgtt gcttatgaca
     1321 aacacaccat tgattttgaa agaaattccg aaggatgaag ccatttcttt tattcggcaa
     1381 taccattatt caaagatact ccctagattg tgtaaatact ttctagggat tttttcagaa
     1441 gaaaaattat taggcgttgt ggaattaggc tggggtacgc aacccctaca aaccatacgc
     1501 aaactctttc ccgacagttc ccttcaaacc acagattatt tagaaattgg aaagatgtgt
     1561 tttcttccag aaatgaatca aaccaattat tttggcagtc aagctctttc agctctaatt
     1621 aagtggttga aagaacatac ggactgtcat tttttatata cactggcaga tggaattgag
     1681 ggaaaatgtg gctatgtcta tcaagcgtcc aacttctttt actgcgggta tttcaagaca
     1741 agtgtctatc gtgataagca gtcatgggaa aagatacacc ctcgaagtgc tcgcctttta
     1801 ttagaggaaa atgctcgctt tgaacaggta gagaaaaagc attggctttc ccaggcattt
     1861 tgcgaataca aagggattga gaaaatcaat ggacgcatgt ttcgctatct ctatccgttg
     1921 acaaaagaag cgaaaaaact gttaggacac actctctatc gacgtcatta ttatccgaaa
     1981 gaaaaaaatt tacgctttga aaagcgaatc gcttatcaga aatatgaagc tatttctcaa
     2041 ccaacttttg ataaacaggc tcgtatctac aatacacaat tattttgata ggtggaaagg
     2101 aggattttta tgccattgct tcatgacaga ggagagcgaa tcaaagccaa cgataagcac
     2161 ttggtctatc attttgtact aatttcattg ctggtagttt ttctagtcgt cctactccct
     2221 tttcattgga gagcgttaca atcggtgaac tggcaagcag ttcagttaga tacatttttc
     2281 tatcaacttc atacgccata cttttggagc agtattctca ctgctctttt tgtatgcgga
     2341 cttatcagct ggctttatta tcactatcgt atagattcta taaaacagtt agagcaccgt
     2401 caaaagttag ctcgtatgat tttagaaaat cattggtatg aagccgaaga aactcaatca
     2461 gagggattct ttaaagattt acccagcaag aaaaccaaac aacgaatccg ccattttcct
     2521 aaaatatatt atcgaatgaa agatggacta atccatattc atgtagaaat caccttaggg
     2581 aaatatcaag atcagctttt gcacctagaa aacaaactag aaacaggatt gtattgtgaa
     2641 ttagtggaaa aaacgttgaa agattcttat gtagagtatg tcctacttta tgatactatt
     2701 ggcaatcgaa ttggcattga agacgtggta gctcaagatg gacgtgtcaa attgatggaa
     2761 tcggtctatt gggagttcga ttctctccct cacatgttaa ttgctggggg cactggtagt
     2821 ggaaaatcct attttattct cacattgatt gaatctctcc tgcatacaaa tgcaaagctt
     2881 tatattcttg accctaaaaa tgctgatttg gcagacttag gggcggtcat gaataatgtc
     2941 tattaccgta aagaagatat gttggcatgt atcaatcaat tttacgaaga tatgattgat
     3001 cgcagtgaaa ccatgaaaca acaccctaac tataaaacag gagaaaatta tgcttatctt
     3061 ggtttgtctg ccaattttct tatttttgat gaatatgtgg caatgatgga tatgctaggt
     3121 cgtgaaagta ccaccgttat tcataaactc aaacaaattg taatgttagg acgacaagca
     3181 ggcttctttt taattcttgc ctgtcaacgt ccagacgcaa aatattttag tgatgggatt
     3241 cgggacaact ttaacttccg tgtggctttg ggtcgtatgt ctgaaatggg ctttggtatg
     3301 atgttcggaa gcgatacaca aaaacaattt ttcttaaaac ccatcaaggg aagaggttac
     3361 gtggatacag gaaaaagtgt gattagtgag ttttatacac cccttgtacc aaaaaggtac
     3421 gatttcttag gagaaatagg aaaagtcatt caaaaaaagc aatctgagcc tgtaccgcac
     3481 gaagtgaaag gtacaggttc agacttgctt tgattggtgt ggcgttagcc acgccaatcc
     3541 tttagccccc tctatctaac aggggggcac aaatacaact gaaaacaggc aattctcatc
     3601 aaccaatgtt gatggtacaa aggattgcgg aaaatatgaa aggtgtaaac tcaagggtca
     3661 tagtttacat cttttttaga ttggaggttt gcgaatggaa aacgaaaaaa tttggattaa
     3721 ggaattgaaa gaaaagcgac tggcttatgg cgtttctcaa aataaactgg cggttgcttc
     3781 tcatattacc agaccttacc tatcggatat tgaaacaggc aaggcagtcc ctactcaaca
     3841 ggtgaaagaa gatttactga acgctttgga acgcttcaat cccgataacc cattggagat
     3901 gttattcgac tatgtacgga ttcgattccc taccaatgat gtggctcaaa ttatcggtga
     3961 ggttttacgt ttgaatatgg attatatgct acacgaagac tttggctatt attcttatcc
     4021 cgaacactat cgtttcggtg atattgttgt cctagtctct catgatgtaa gtaaaggtgt
     4081 tttattagaa ctgaaaggaa aaggctgtcg gcaatttgaa aactttctat tggctcaaca
     4141 tcggagctgg tatgatttct ttcaagattg catggaacat aaaggtattt tcaaacgctt
     4201 ggatttagct atcaacgata aaacagggat tttgaatatt ccagatttgg caaagaaatg
     4261 taaacaggaa gaatgcattt ccgtctttcg aagctttaaa aattatcgca gtggtgaatt
     4321 ggttcaccgt gatgaaaaac catacatggg aaacacactt tatattggct cattaaaaag
     4381 tgaagtctat ttctgtatat atgagaaaga ttatgagcaa tacgtgaaaa atgatgttcc
     4441 cttagaagac gcagaagtaa agaatcggtt tgaaattcgg ttaaaaaatg accgagccac
     4501 ccaagccatg aaagatttat tggcacatca acaggcggaa aaaacagcct ttgaaattat
     4561 caatcgctat atccgctttg cagataaaga cgacactaaa cgtcggagtg attggaaaac
     4621 taatgaacgt tgggaatggt ttatcggtaa aaatcgtgga gcattacgcc taaccacaca
     4681 gccagagcct tattcttttg aacgaacatt gaactggtta catcatcaag tcgcccctac
     4741 attaaaaatc gcaagcattt tagatgttct caacggtaca accattatct ccaccatgat
     4801 tcaggaagcc aaactgacag aaaaacatga gaaattaatt gaacaacaac atctagccat
     4861 ggaggatttg attacatgag ccttttaaca aaagaattaa agaagttagg gtttcagtgt
     4921 ggcattgagt ttcaagcgta tatacaaaat acagggaaat atacgtctct cattattgag
     4981 ggaaaacggc aagctggtga taccatctat acctatgatt tttacaaggt gagattttat
     5041 aataattata ccaatcgagt aaccgtctat ggagagcatt taaccccttt tcaattatta
     5101 aggcgtgtaa aaagctatat ctattatcga gaaaaatatc ttaaggaaag gaggacaatc
     5161 acatgaattt tgggcagaat ttgtacaact ggttcttgga taatgcacag tcattggtat
     5221 tgttagcgat tgttgtcatt ggactgtttt taggttttaa acgagaattt tcaaaactca
     5281 ttggcttctt gattattgct ctaatcgccg tcggacttgt gttcaatgca gctggcgtga
     5341 aagatgtgtt attaaactta tttaaccgaa tcattggtgc ttaacagata tttttcatga
     5401 taaaatagga agtatatatt ttgttatgaa aggatttatg aatatgttta caaatgatca
     5461 atttaaatct cttttaatta aaacattaga aagaaagagc tcttcaaatt attatgaagg
     5521 aggtaacgag attgtttcta aaatgaaaat atcagaggta actttagatg agactgacga
     5581 ttttacttat tataaaggat ataaaaaggg ctggaatacg ctttgcacat atcttaaaat
     5641 aagagttcct tttgatgatt tagacttttt tgaaagtcat aaagatttga ttacccagac
     5701 agcaagctcc atatatgata aacaaggtga taatgtatta gtggatacta ttttagtacc
     5761 acttccagag aattacgaag taattaactt tagtcagttg aaaattagtg atgtggtttc
     5821 acaagctatt gaagacgctg agtcttttat gagtaatggt gaatatcaac gtgcctttga
     5881 cagagttcac actgcttttc atggatattt aattgaaata ctaaaaaaat atgaaattac
     5941 agttcctaga gacgaaaatc tatctaaatt atatagtagg atacaacaat taattgaaaa
     6001 agaaattcaa cctactgaac ttgctgatat agtgaagaca acgataaggt catcaaatgg
     6061 aatgattagc tctcttaatg aagcaagaaa taggcattca cttgcacacc ctaatacaaa
     6121 tattattgga aagagagaag caaaactaat aataggtatt tcaagtacag ttacagatta
     6181 tattagcgga taccttgata agtagcgtca ttaactgatt gctacttttt ttatgactat
     6241 ttttataaaa aggatgtgat aagaattgga aatgaaagtc tatgtggcaa atttaggacg
     6301 gtataatgaa ggtgaattag tcggagcatg gtttactcca ccaattgatg aggaagaaat
     6361 ggcagaaaga atcggtttaa atgaggacta cgaagaatat gcaatacatg attttgagct
     6421 tccttttgat gtggacgaat acacgccgat tagtgaaatc aaccgtctat gtgaagccat
     6481 tcaagaaatc gaaggtacac ctatttataa tgaactcaaa gaaattcaag gcatgtggtt
     6541 tagtagcttg gaagaattac tggaaaacaa agaggatatt cattgttata gtgattgtga
     6601 ttcgatggaa gatgtcgccc gttattacgt ggaagaaaca ggacagcttg gtgaagtccc
     6661 ttccaattta caaaattata ttgactacca atctttggga cgtgatatgg aaatagaggg
     6721 taattaccta gttacctctc atggggtatt tgaatattgt cagtaagttt aacaatccgt
     6781 gttataatta aattaataaa tagcacggag aggtataaat tatggtaaat gtatttgaaa
     6841 atcttaaact attaaaacta gacatgaaag ataaaggctg ggtgatagat tccttttact
     6901 ttcgttacaa acagcaaaat tatattgtac ttgttaagct atttgaaaaa gaggaaaaag
     6961 ttccagaata tgctctgtta aaactagaat ttttaaagga aaacgatttt tcagatatgc
     7021 tagctgtcta tgctaattcg gtaaaattat atacagacac gaaaacaata agagaatatt
     7081 ttggtatcga atacagtagt aacttgggag atgttttatt tcaatttagc caaactcttg
     7141 ctcgatttat tccgacagaa gttagtgaga agaaaaatga agaccaaaaa gaagccatgt
     7201 gtttctcatt aagtcaaagt gattcagaag atcccagaaa aaaatattgt ttttcagtta
     7261 gaagaaatcc tttaaaggga aatggtgagc tgggaaaacg aagtcctttt aatgacaata
     7321 aaacgagatt gtatagaccc agcttatatg aacgattagg gtcagatacc aatttaagtt
     7381 tcagatattc aatgaatcca gatgatgaag aaactgatga aggtattatt gcaaaatgga
     7441 caaaaaataa aattgaatag ttaaagcatt gaaagagcaa tcacctattt tgattgttct
     7501 ttttctattt ttacggaaag gacgtgaaga catgaaaaaa ataagaagtt acaccagtat
     7561 ttggtcagta gaaaaagtga tttatgcgat taatgattta caactccctt tcccaattac
     7621 ctttacgcaa atgacatggt ttatcgtttc tttgtttgtc gtcatactct ttggcagttt
     7681 accaccactt tctctgattg acggagcact actcaaatac ttagggattc ccattgttct
     7741 cactgggttc atgtgccaaa agacctttga cggaaagaaa ccctatggat ttctaaagtc
     7801 tatcattacc tatgctctac gccctaaagt aacctacgct ggcaaacaaa tacgattaaa
     7861 aaaagaaaca atgaatgaat cgattacgat tgtaaggagt gaacaactag atggcatatc
     7921 cgattaaata tatggaagat aatctcgtat ttaatcatga tggcgaagta ttcgcttatt
     7981 atgaattaat tccctataat tacagttttt taagttccga tgaaaaaatt caagttcatg
     8041 ataatttctg tcaattgatt gcccaaaatc gtgatggcaa gattcatgct ctgcaactag
     8101 caaccgaatc aagtattcga gccacacagg aacaatcgaa aaaagaaatc actggacgat
     8161 taaaagagat agcttacgaa aaaatagatg aacagaccga agcgttaatt tctatgattg
     8221 gtgacaatca agtagactat cgctttttta ttggttttaa actctacctc aatgaacagg
     8281 aagtatccgt aaaaaatgtt gggaaagaaa ttcaaaaagc atttgcggaa ttctttcatg
     8341 gtgtgaatca caaattaatg ggcgattttg tttctatgcc tatggcggaa atcgaacgct
     8401 tttctaagat ggaacagttg ttagaaagta aattatccag acgctttcag attcgtaaat
     8461 tggagaaaga tgattttggt tatattattg aacatcttta cgggcaaaca ggtgtccctt
     8521 ttgatgacta cgactaccat ttaccgaaac gatacgaaga taaaaaagtg ttggtaaaga
     8581 aatacgatat tctcaaaccc actcgctgtt taatcgaaga aaatcaacgc tatttacgga
     8641 ttgaacagga agataccact acttatgtgg cgtattttac aattaatagt attgttggtg
     8701 aattggattt ccctaatagt gaaatttttt attaccagca acagcagttt actttccccg
     8761 ttgataccag tatgaatgtt gaaattgtcg ccaataaaaa agccttatct accgttcgca
     8821 ataaaaagaa agaactgaaa gatttagata gccacgcatg ggaaagcgac aacgaaacca
     8881 gtactaatgt ggtggacgct ttagaatctg tcgatgaatt agaaagtaat ttagaccaaa
     8941 ccaaagaatc tatgtacaaa ctaagctatg tggtacgggt atcagcaaag aatttggacg
     9001 agctgaaaca acgttgtgat gaagtaaaag atttttatga tgatctgtct atcaaattag
     9061 tacgaccttt tggcgatatg ctaggcttgc ataatgaatt tattccctca agcaagcgat
     9121 atatcaatga ctatattcaa tatgtcacgt cggatttttt ggctggtctt ggtttcggtg
     9181 ctacacaaat gattggtgaa aattctggta tttacgtcgg ttacaatctc gatacaggca
     9241 gaaatattta tctccaacct agccttgcaa gtcaaggaat taaaggctct gtaacaaatg
     9301 cattagcgtc cgcttttatt ggttcattag gtggcggaaa atcattctct aacaatatgc
     9361 tggtctatta ttcagtactc tttggcggac aggcaaccat tgttgaccca aaagctgaac
     9421 gtggtgaatg gaaagaaacc ttacctgata ttgcccatga aatcaatatt gttaatctca
     9481 cgagtgagaa cgacaataaa gggttacttg acccttatgt gattttaaaa ctcccgaaag
     9541 attcagaaag ccttgccatt gatattctca cgttcttaac aggtatttcc agccgagata
     9601 gcgagaagtt tcctgtcctg cgtaaagcta ttcggagtgt aactcaaagc aaacaacgag
     9661 ggttgttatt ggtgattgat gaattgcgaa aagaagatac gcccatcagc aatagtattg
     9721 ctgaccatat cgaaagcttt gtcgattatg attttgccca tctgttattc agtgatggca
     9781 gtgtcaccca atccattagc ttggaaaaac aactcaatat tattcaagtg gcagatttgg
     9841 tcttacccga ttccaatagc tcttttgaag aatacactac catggaatta ttaagtgtgg
     9901 caatgctgat tgtcattagt acctttgctt tagactttat ccactctgac cgttccattt
     9961 ttaaaattgt tgaccttgat gaggcttgga gttttttaca agtggcacaa ggaaaagctc
    10021 tatctatgcg attggttcga gcaggacgtg ccatgaatgc aggggtctat ttcgttactc
    10081 aaaacgcgga cgatttacta gatgaaaaga tgaagaataa tattggcttg aaatttgcct
    10141 ttcgttccac tgacctagtg gaaatcaaaa agaccttaga attttttggt gtggacaagg
    10201 aagatgaaaa caatcagaaa cgcttacgtg aattagaaaa tggacaatgt ttagtttcag
    10261 acatctatgg tcgtgtaggg gttatgcagt ttcacccgat ttttgaagag ttgcttcatg
    10321 cctttgatac cagaccgcct gtgcgaaatg aggtggaaga atgacacaaa aacaaaagaa
    10381 atttcttcac tatagttgga taacgctcct ttgtgtgggc gttattttac ttttattagg
    10441 cacgctgggc aatgcagtac aagcgacagg cttagttgat gaaaccattg atacaagcaa
    10501 tgaatactcc aagtatggtt tgaatcaata tcagcttgat tattatgtgg acaatagctg
    10561 gggctggctt ccatggaatt ggagtgatgg cataggacaa tcggtgatgt atggattgta
    10621 tgccattact aattttattt ggacaatttc tctttatcta tcgaacgcca caggttattt
    10681 ggtgaaagaa gcctatgcct tagatttcat tagtcaaaca tcccaagcaa ttggcgaaaa
    10741 catgcagacg attgcaggca ttacaccgaa tggcttttcc agttcagggt tttatgtcgg
    10801 tttcctcctt ctttttattc tcattgttgg aatctatgtt gcctatacag gattggttaa
    10861 gcgagaaacg acgaaagtga tacgagctgt tttaaacttt gtcgtggtgt ttattctatc
    10921 ggcttccttt attgcgtatg cacccgatta tattgcgaaa atcaatgatt tttccaaaga
    10981 tgtcagtacc gccagtctga atattgggac aaaaattgtt cttcctaaca cggatagtca
    11041 agggaaagac agtgtggaca tgatacgtga cagcttattc tctgttcaag taaagcaacc
    11101 atggctattg cttcaatatg gctccactga tgtagaagcg ttaggggaag aacgtgtgga
    11161 aagtttattg tctaccagtc cagatacaaa caatggtgaa gatagagaaa atgtagtgat
    11221 tgaagaaatt gaagatcatg acaataccta tttaacccta ccgaagacga tttccagatt
    11281 aggaacagtc ttctttttgt ttgtattcaa tattggtatc tcgatttttg ttttccttct
    11341 aacaggaatc atgatttttt cgcaagtgct atttattatt ttcgctatgt ttttgccaat
    11401 cagtttccta ttaagtatgt tgccgagttt tgatggcatg ggcaggcatg ccattaccaa
    11461 gttatttaat gtgattatga caagggctgg tattaccttg attattaccg ttgcctttag
    11521 tatttccact atgctttatt cgctgtcagc aagttctccc tttttcatga ttatgttttt
    11581 acaaattgtg acctttgcag ggatttattt taaactgggc gatttaatga gcctattcac
    11641 tttacaaagc aacgactctc aaaacatggg cagacaagtg tttcgcagac ctcgtcaaat
    11701 gatgaatcga caaaccagaa gatttcaacg cagtatgagt aagatttttc gtggaaaagc
    11761 agggtcaacg aagcaaaaga caatgacaac aaagcagaat caaaccaata aagctaacca
    11821 tactcgtcaa aatgaacgga ctacaccaaa gaaagaaaca gcactttcca atgtgaaaaa
    11881 gcaacaaggc ggtatcgggc aacgattagg tgaaaagaca gcccatgtat tggacacgaa
    11941 aaatcgtcta gtcaataaaa gaaagcaggc aaaacaacag atacaggcga caccaaccca
    12001 tgtcaaatat gcccttcatc aaggaaaaga gaatgtcact cataatgtaa cagactttaa
    12061 acaatctatg gcagatacca aagctcaacg caaacaacag cgtatgaacc agcaggagca
    12121 aaaacgaaag aacattgctc aaaagcgttt ggagctggat aaagcaaaag aaaaaagacg
    12181 aaaagaaaac ccatcatctc ctgttcagaa taaccagcag catgttcgca agcgtcagca
    12241 aaccgtcacg cctgttttgt cacaaaacac taaacgtgaa aatcatcagc gaccattgaa
    12301 aaaagagcaa aaacaaaaga caccaccgaa gcaattcaat cagaggaagc taaataaaag
    12361 aacggataag cgaggacaac aaacatgaag ctaaaaataa cagtgatttg tagtgtggtc
    12421 ttctctctct tcttttttct cttattattc ctagtcattt tctttgcaga tgatacggac
    12481 agtggcgaaa acaacaagga ttcttctatt ccacaaggcg gtgtgaccgt ttcaccagaa
    12541 gtgttggcac accgtccgtt gattgaaaaa tatggtaagg aatatgggat tgaagactat
    12601 gtttcttata tcttagccat tatgcaagtg gaatcgggcg gtacagcaga agatgttatg
    12661 caaagttccg aatctttggg tttaccgccc aacagtttga gtaccgaaga gtccatcaaa
    12721 caaggggtta aatatttcag tgaattatta accagtgcag aacaacaagg agtggatata
    12781 gattctgtta tccaatctta taattatggc ggtggctttt taaattatgt gaggagtcat
    12841 gggaaaaaat atacctatga attagccgaa cagttttcta aagagaagtc gggtggtcaa
    12901 aaagcagact atcccaatcc tatagccata cctgtgaatg gtggttggcg atataactat
    12961 ggaaatcagt tttatgttca gttggtttca caatacctaa ccgacacttc cccaacagaa
    13021 tttgatgatg aaaccgtgca agtcattatg gacgaagcct tgaaatacga agggtttccc
    13081 tatgtctttg gaggggcgtc gcctaccacc tcttttgatt gtagtggctt aatacaatgg
    13141 gtgtatgaca aagctggcat ttctctccct cgtgtcgccc aagatcagta tgacgctacg
    13201 caagaaatct ctatggaaga agcccaagca ggcgacctta tcttctttca ttcaacctat
    13261 aatgcaggca catatgtgac tcatgtggcg atatatttag agggaaatcg cttttatcat
    13321 gcaggcgacc caatcggtta tggcgactta agcagtcgtt attggcaaga ccatctgatt
    13381 ggggctagac gtgttattca taactaagaa aggaatgaat caaatgatta taaaaatcga
    13441 gcgaaaagaa aagaaggaga aaccaaagaa atctaaaaaa ccgagcaagc aaagaaaagt
    13501 tccgatgatt aaagttggca cgcataagaa attaaccttt gttttatggg ttttattgat
    13561 tggtagtgtc ggatttggaa tctataaaaa ttttactgcc attgacactc atactgtccg
    13621 tgaaacagaa atcattaaac agcagattgt cgataccaac caagtagaaa gttttgtgaa
    13681 atcctttgcg aaagactact tttcgtggca acaatcccaa gaagccattg ataaacgaaa
    13741 tgaaaaattg acccattatt tgacggaaga attgcaggta ttaaatgaag aaatgattcg
    13801 taaggatatt cctactagtt ctagtgtgaa cgatatacag gtgtggcagg tttctcaagt
    13861 gaatgagaac acctttgagg tactgttttc tgttgaacaa gttattaccg aagacaaaga
    13921 caaggaaact atatcgtcta gctttcatgt ggttgtccat atagatgaat cagataatat
    13981 ggttattatc aaaaatccga caatgagtaa gaaacctcaa aaatcggact accaacccaa
    14041 acagctagaa agtgaccata cagtagatac agaaacgatg gacgaaatca ctagcttttt
    14101 agaaacgttc ttccagctct atcctaccgc cacagaaaaa gaattgactt actatgtaag
    14161 taatcatgtg ttacctatga ttaacaagga atatgttttc gaggaattgg tgaatcctat
    14221 ctttaccaga aaagataacc aagtaatcgt gaatgtcgct gtgaaatatt tagatcaaga
    14281 aacaaaggca acccaaatct cgcaatttga gttgatatta gaaaaacaag ataattggaa
    14341 gattgtaaaa taacaaatat tggtacatga ttacagatac tttgtaatca tgtactcttt
    14401 ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtgac
    14461 tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat
    14521 agcgggaaca aataattaga tgtccttttt taggagggct tagttttttg tacccagttt
    14581 aagaatacct ttatcatgtg attctaaagt atccagagaa tatctgtatg ctttgtatac
    14641 ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat ttttatgccc
    14701 ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc
    14761 atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta
    14821 cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc
    14881 agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga
    14941 acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag
    15001 ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta
    15061 tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg
    15121 accaaaatgg aattgattta tcaacggttt atcag
//
LOCUS       DAPBBG010000062        14392 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPBBG010000062 DAPBBG010000000
VERSION     DAPBBG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.065"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..556)
                     /locus_tag="RD477_002318"
     CDS             complement(<1..556)
                     /locus_tag="RD477_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDT7634643.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="RD477_002319"
     CDS             complement(572..1051)
                     /locus_tag="RD477_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDT7634644.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="RD477_002320"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="RD477_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="RD477_002321"
     CDS             complement(2098..3204)
                     /locus_tag="RD477_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDT7634645.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="RD477_002322"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="RD477_002322"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDT7634646.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="RD477_002323"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="RD477_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDT7634647.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="RD477_002324"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="RD477_002324"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDT7634648.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="RD477_002325"
     CDS             8175..8873
                     /locus_tag="RD477_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDT7634649.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="RD477_002326"
     CDS             complement(9041..11680)
                     /locus_tag="RD477_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7634650.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="RD477_002327"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="RD477_002327"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDT7634651.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14392
                     /locus_tag="RD477_002328"
     CDS             13523..>14392
                     /locus_tag="RD477_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7634652.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHTANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSN"
BASE COUNT         4060 a         3148 c         2601 g         4583 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
    14161 tgtgatccaa tttgatacac cagaaaatat tttacgtcat actgcaaacg atttcgtaga
    14221 agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga
    14281 agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa
    14341 actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga at
//
LOCUS       DAPBBG010000063        14105 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPBBG010000063 DAPBBG010000000
VERSION     DAPBBG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.066"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            66..767
                     /locus_tag="RD477_002329"
     CDS             66..767
                     /locus_tag="RD477_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDT7634653.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="RD477_002330"
     CDS             764..1885
                     /locus_tag="RD477_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDT7634654.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="RD477_002331"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="RD477_002331"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDT7634655.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="RD477_002332"
     CDS             3264..4172
                     /locus_tag="RD477_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDT7634656.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="RD477_002333"
     CDS             4207..4479
                     /locus_tag="RD477_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDT7634657.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="RD477_002334"
     CDS             4490..5536
                     /locus_tag="RD477_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDT7634658.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="RD477_002335"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="RD477_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDT7634659.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="RD477_002336"
     CDS             complement(8547..9233)
                     /locus_tag="RD477_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634660.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="RD477_002337"
     CDS             complement(9211..9705)
                     /locus_tag="RD477_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDT7634661.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="RD477_002338"
     CDS             complement(9725..10945)
                     /locus_tag="RD477_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7634662.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="RD477_002339"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="RD477_002339"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDT7634663.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14105)
                     /locus_tag="RD477_002340"
     CDS             complement(13021..>14105)
                     /locus_tag="RD477_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7634664.1"
                     /translation="INDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4554 a         2592 c         2920 g         4039 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatat
//
LOCUS       DAPBBG010000064        13816 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPBBG010000064 DAPBBG010000000
VERSION     DAPBBG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.067"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="RD477_002341"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="RD477_002341"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDT7634665.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="RD477_002342"
     CDS             complement(2260..2511)
                     /locus_tag="RD477_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDT7634666.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="RD477_002343"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="RD477_002343"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDT7634667.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="RD477_002344"
     CDS             3461..3679
                     /locus_tag="RD477_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634668.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="RD477_002345"
     CDS             complement(3968..4480)
                     /locus_tag="RD477_002345"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDT7634669.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="RD477_002346"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="RD477_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDT7634670.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="RD477_002347"
     CDS             complement(6933..7376)
                     /locus_tag="RD477_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDT7634671.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="RD477_002348"
     CDS             complement(7377..8165)
                     /locus_tag="RD477_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7634672.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="RD477_002349"
     CDS             complement(8162..9103)
                     /locus_tag="RD477_002349"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDT7634673.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="RD477_002350"
     CDS             complement(9280..9867)
                     /locus_tag="RD477_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDT7634674.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="RD477_002351"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="RD477_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDT7634675.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11698..12708
                     /gene="gap"
                     /locus_tag="RD477_002352"
     CDS             11698..12708
                     /gene="gap"
                     /locus_tag="RD477_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDT7634676.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12793..13728)
                     /locus_tag="RD477_002353"
     CDS             complement(12793..13728)
                     /locus_tag="RD477_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7634677.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3810 a         3095 c         2436 g         4475 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg
    11701 acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc
    11761 aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg
    11821 ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac agttactgca
    11881 acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca
    11941 agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt
    12001 tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt
    12061 tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat
    12121 gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct
    12181 tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac
    12241 acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt
    12301 tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt
    12361 atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt
    12421 tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa
    12481 gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt
    12541 tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta
    12601 acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga
    12661 tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca
    12721 atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac
    12781 ttttaaactc gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt
    12841 ttcaggatct gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt
    12901 tggtcccttc gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc
    12961 gtctccatgt ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc
    13021 ttcaccgaaa aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg
    13081 aaatgctgga agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga
    13141 ataaccgcaa tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat
    13201 cataactgtt gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt
    13261 gttttctagc actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc
    13321 aatgtaatcc ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc
    13381 catatcatga aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg
    13441 agccacaaga gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt
    13501 cagaccgtct tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt
    13561 atgtgcatcc gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc
    13621 tttctcagaa ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac
    13681 aatcagagct aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc
    13741 tcatttctct tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa
    13801 tgttcatttt tataac
//
LOCUS       DAPBBG010000065        13778 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPBBG010000065 DAPBBG010000000
VERSION     DAPBBG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.068"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(45..1364)
                     /locus_tag="RD477_002354"
     CDS             complement(45..1364)
                     /locus_tag="RD477_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7634678.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="RD477_002355"
     CDS             complement(1361..2014)
                     /locus_tag="RD477_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634679.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="RD477_002356"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="RD477_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="RD477_002357"
     CDS             complement(2724..3440)
                     /locus_tag="RD477_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDT7634680.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="RD477_002358"
     CDS             complement(3564..3749)
                     /locus_tag="RD477_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634681.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="RD477_002359"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="RD477_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="RD477_002360"
     CDS             complement(5356..6387)
                     /locus_tag="RD477_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634682.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="RD477_002361"
     CDS             complement(6394..7224)
                     /locus_tag="RD477_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634683.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="RD477_002362"
     CDS             complement(7221..8036)
                     /locus_tag="RD477_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7634684.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="RD477_002363"
     CDS             complement(8033..8857)
                     /locus_tag="RD477_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDT7634685.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="RD477_002364"
     CDS             complement(8847..9947)
                     /locus_tag="RD477_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7634686.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="RD477_002365"
     CDS             10125..10910
                     /locus_tag="RD477_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDT7634687.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="RD477_002366"
     CDS             10943..11326
                     /locus_tag="RD477_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDT7634688.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="RD477_002367"
     CDS             11402..11533
                     /locus_tag="RD477_002367"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7634689.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="RD477_002368"
     CDS             11502..11738
                     /locus_tag="RD477_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7634690.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="RD477_002369"
     CDS             complement(11816..12787)
                     /locus_tag="RD477_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDT7634691.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13439..>13778
                     /locus_tag="RD477_002370"
     CDS             13439..>13778
                     /locus_tag="RD477_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7634692.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
BASE COUNT         4179 a         2995 c         2388 g         4216 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agtttctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tactccttcg aggagaaaat
    13741 cccccatttc tcaacttttg gaaagaatta tgttcgcc
//
LOCUS       DAPBBG010000066        13428 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPBBG010000066 DAPBBG010000000
VERSION     DAPBBG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.069"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..914)
                     /locus_tag="RD477_002371"
     CDS             complement(<1..914)
                     /locus_tag="RD477_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDT7634693.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1341..1904
                     /gene="efp"
                     /locus_tag="RD477_002372"
     CDS             1341..1904
                     /gene="efp"
                     /locus_tag="RD477_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDT7634694.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2005..2175
                     /locus_tag="RD477_002373"
     CDS             2005..2175
                     /locus_tag="RD477_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634695.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2390..3649
                     /gene="lysA"
                     /locus_tag="RD477_002374"
     CDS             2390..3649
                     /gene="lysA"
                     /locus_tag="RD477_002374"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDT7634696.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3636..4436
                     /gene="dapF"
                     /locus_tag="RD477_002375"
     CDS             3636..4436
                     /gene="dapF"
                     /locus_tag="RD477_002375"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDT7634697.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4439..5320
                     /locus_tag="RD477_002376"
     CDS             4439..5320
                     /locus_tag="RD477_002376"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDT7634698.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5317..6033
                     /locus_tag="RD477_002377"
     CDS             5317..6033
                     /locus_tag="RD477_002377"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDT7634699.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6039..6734
                     /gene="dapD"
                     /locus_tag="RD477_002378"
     CDS             6039..6734
                     /gene="dapD"
                     /locus_tag="RD477_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDT7634700.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6750..7751
                     /locus_tag="RD477_002379"
     CDS             6750..7751
                     /locus_tag="RD477_002379"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDT7634701.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7748..9109
                     /locus_tag="RD477_002380"
     CDS             7748..9109
                     /locus_tag="RD477_002380"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDT7634702.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9069..10220
                     /locus_tag="RD477_002381"
     CDS             9069..10220
                     /locus_tag="RD477_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDT7634703.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEIIQSI"
     gene            10195..11364
                     /locus_tag="RD477_002382"
     CDS             10195..11364
                     /locus_tag="RD477_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7634704.1"
                     /translation="MMKLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11539..12918
                     /locus_tag="RD477_002383"
     CDS             11539..12918
                     /locus_tag="RD477_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7634705.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13199..13428
                     /locus_tag="RD477_002384"
                     /pseudo
     CDS             13199..13428
                     /locus_tag="RD477_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3680 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaat aaaagtcaaa ggcttgaaag aaaaaaatat
     1201 ttcgatagca tcgtttaccc gtgtttattt ttctgacaaa taggcatgag accttaaaag
     1261 atagagaata ctctagtttt ctggccggtt gtctgataca ataaagttga ttatgataaa
     1321 gaaaagaagg agcaaaaata atgatctcag caagtgattt aagagccggt atgactttcg
     1381 tacaagacgg caaactgatt aaagttctgg atgcaagcca ccataaacct ggtaaaggaa
     1441 atacagttat gcgtatgaaa ctgaaagatg tccgttcagg tgcaacatac gacacaacgt
     1501 tcagaccaga agaaaaattt gaaaaagcac atatcgacac aaaacctgta caatatttgt
     1561 acacgatgga tgatacagct ttcttcatgg atttggaaac atatgagcaa tatgaaattc
     1621 ctgtagaaac agtagaagaa gaaatgaaat atatcctaga aaacatggaa gtaaaaatcc
     1681 aatttttcgg tagcgaagtc atcggcgttc aattaccaac aactgtcgta ttacgtgttg
     1741 tggaaacaca accttccatc aaaggtgcaa cagtcactgg ttcaggtaag ccagcgacaa
     1801 tggaaactgg cttagtagtc aatgttcctg acttcatcga aacagatgaa ttgttggaaa
     1861 tcaatacagc agaaggttct tatgtaaaac gtgcgtcaaa ataattcgct aacggattga
     1921 taagaatttg aaaacagagc ttgacctctt gaaaggcaga tcatcggatc tgcctttcaa
     1981 gaggtttttt gtggaatcag caaattgaaa aataaaaaag tatttgattt tatttatatt
     2041 ttgaaagatg aatggcaaaa gctagagaaa gaagaaactg cacttattta tgaagaaatt
     2101 gaaatgattc ataaaattga aaatatccga actattccga actatattga agcgaaaata
     2161 ttttttcatc actaaatagt gttgaattta tgtttttatg tataaatagc ttctaatagt
     2221 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
     2281 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
     2341 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
     2401 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
     2461 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
     2521 acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca
     2581 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
     2641 tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga
     2701 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
     2761 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
     2821 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
     2881 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
     2941 aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
     3001 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
     3061 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat
     3121 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
     3181 aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg
     3241 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     3301 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     3361 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     3421 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     3481 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     3541 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     3601 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     3661 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     3721 cttctttatg cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc
     3781 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     3841 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     3901 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     3961 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     4021 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     4081 taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     4141 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     4201 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     4261 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     4321 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     4381 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     4441 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     4501 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt
     4561 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     4621 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     4681 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     4741 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     4801 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     4861 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     4921 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     4981 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     5041 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     5101 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     5161 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     5221 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     5281 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     5341 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     5401 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     5461 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa
     5521 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     5581 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     5641 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     5701 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     5761 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     5821 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     5881 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     5941 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     6001 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     6061 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     6121 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     6181 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     6241 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     6301 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     6361 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     6421 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     6481 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     6541 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg
     6601 cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg
     6661 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     6721 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     6781 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     6841 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     6901 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     6961 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     7021 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     7081 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     7141 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     7201 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     7261 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     7321 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     7381 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     7441 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     7501 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     7561 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     7621 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     7681 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     7741 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     7801 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     7861 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     7921 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     7981 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     8041 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
     8101 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
     8161 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
     8221 cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc
     8281 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
     8341 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
     8401 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
     8461 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
     8521 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
     8581 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
     8641 tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc
     8701 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
     8761 tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga
     8821 attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc
     8881 tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt
     8941 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
     9001 catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt
     9061 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc
     9121 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc
     9181 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
     9241 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
     9301 ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
     9361 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
     9421 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
     9481 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
     9541 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
     9601 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
     9661 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
     9721 taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta
     9781 ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
     9841 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg
     9901 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
     9961 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
    10021 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
    10081 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
    10141 cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg
    10201 aaattatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa
    10261 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
    10321 tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct
    10381 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa
    10441 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
    10501 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
    10561 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
    10621 atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
    10681 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
    10741 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
    10801 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
    10861 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
    10921 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
    10981 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta
    11041 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
    11101 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
    11161 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
    11221 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
    11281 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc
    11341 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
    11401 gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct
    11461 tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata
    11521 ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga
    11581 gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta
    11641 tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac
    11701 aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat
    11761 gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg
    11821 gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt
    11881 tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg
    11941 atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa
    12001 aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa
    12061 gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat
    12121 gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca
    12181 aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa
    12241 aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg
    12301 ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa
    12361 acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca
    12421 cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta
    12481 aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca
    12541 ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa
    12601 gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca
    12661 gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa
    12721 ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca
    12781 ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag
    12841 ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa
    12901 gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt
    12961 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga
    13021 cttattaccg cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca
    13081 cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat
    13141 tttttatggt taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg
    13201 gcaaaggcga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat
    13261 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
    13321 tttctctatc aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac
    13381 ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAPBBG010000067        13147 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPBBG010000067 DAPBBG010000000
VERSION     DAPBBG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.070"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(77..149)
                     /locus_tag="RD477_002385"
     tRNA            complement(77..149)
                     /locus_tag="RD477_002385"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="RD477_002386"
     CDS             530..1099
                     /locus_tag="RD477_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDT7634706.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="RD477_002387"
     CDS             1086..1946
                     /locus_tag="RD477_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7634707.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="RD477_002388"
     CDS             2136..2840
                     /locus_tag="RD477_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634708.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="RD477_002389"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="RD477_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDT7634709.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="RD477_002390"
     CDS             4677..5993
                     /locus_tag="RD477_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634710.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="RD477_002391"
     CDS             5994..6863
                     /locus_tag="RD477_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDT7634711.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="RD477_002392"
     CDS             6918..7727
                     /locus_tag="RD477_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7634712.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="RD477_002393"
     CDS             complement(7830..8459)
                     /locus_tag="RD477_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDT7634713.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="RD477_002394"
     CDS             8629..9921
                     /locus_tag="RD477_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634714.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="RD477_002395"
     CDS             complement(9997..10659)
                     /locus_tag="RD477_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7634715.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="RD477_002396"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="RD477_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDT7634716.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="RD477_002397"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="RD477_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDT7634717.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DAPBBG010000068        13118 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPBBG010000068 DAPBBG010000000
VERSION     DAPBBG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.071"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1401)
                     /locus_tag="RD477_002398"
     CDS             complement(<1..1401)
                     /locus_tag="RD477_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDT7634718.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKN"
     gene            complement(1398..3587)
                     /locus_tag="RD477_002399"
     CDS             complement(1398..3587)
                     /locus_tag="RD477_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7634719.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3721..4911)
                     /locus_tag="RD477_002400"
     CDS             complement(3721..4911)
                     /locus_tag="RD477_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDT7634720.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5783..7414)
                     /locus_tag="RD477_002401"
     CDS             complement(5783..7414)
                     /locus_tag="RD477_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDT7634721.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7428..9101)
                     /locus_tag="RD477_002402"
     CDS             complement(7428..9101)
                     /locus_tag="RD477_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDT7634722.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(9114..9431)
                     /locus_tag="RD477_002403"
     CDS             complement(9114..9431)
                     /locus_tag="RD477_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7634723.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9456..10856)
                     /gene="lacG"
                     /locus_tag="RD477_002404"
     CDS             complement(9456..10856)
                     /gene="lacG"
                     /locus_tag="RD477_002404"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDT7634724.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            11093..11878
                     /locus_tag="RD477_002405"
     CDS             11093..11878
                     /locus_tag="RD477_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7634725.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11881..12573
                     /locus_tag="RD477_002406"
     CDS             11881..12573
                     /locus_tag="RD477_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7634726.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12847..>13118)
                     /locus_tag="RD477_002407"
     CDS             complement(12847..>13118)
                     /locus_tag="RD477_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDT7634727.1"
                     /translation="SSGISDYIDMSGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDT
                     YLVGKLINILGTEKDTETFISSLSMLFTDKHFSTQLGGEELVEIF"
BASE COUNT         4031 a         2681 c         2153 g         4253 t
ORIGIN      
        1 atttttctcg tcaatattaa gattaacaaa tatatgtttg gtaacaaatt tgtataaatc
       61 aacaccgttg tcgtctccac tatacattct tttcacactg tttattagct gtaataaatc
      121 taagtcaata aaataatctt taacttctat tccaaattca ttttttaata taattaaaat
      181 atcgtttgca attagctttc ttcttcgaac cttttcacct gattcagtaa agtacaaaat
      241 tgctaacttg aaaaataaac tatgattatt taccttaatt ggtgatatat ttatagcatc
      301 aaaattgcaa tacggtaatt ctttctttaa atactcaccc agtgaaaaca gaggatagtg
      361 ttgaggagca agtacacaaa tatctttttc atgaatgccg ctttcaatct tttcctttat
      421 tatatttttt attatgttgg gtaaatcctc cttagtaact ttgcaatcat aacttataaa
      481 accacggtcg tttttattat tccctctgct ttcaatctcg taaggacaac tctgaaaatt
      541 agagtagaag tctactaatc tctgattaga cctgtaacag ccatgtagcg tctttgattg
      601 aaaagtgtga ctagttagca tttctagttc ttcaactgat ttagctatac catctaatga
      661 tccataaatt gcttggtcaa tatctccaac aaacattgac tgaatttgag ggttgttttg
      721 aagtaattta gctattacat tgtattgtaa ttctctagta tcttgaaatt catccacata
      781 aatcgccctt atattactag caatattacg tgcaacgtta ctgtttacag ttaaaatttt
      841 atacgctact agtaaaatca tatcaaaatc aatttcttta tttttttcca acatagtgtg
      901 atacatttca gaaacttgag tatactgaac ttcattaatt ttaaaatcat gagaaagtga
      961 caaatcaatt ttgttcagtt catacgtttt gagatttaaa ttcagcatat tattaatttg
     1021 gctgacgtaa gctctttgag tataatcatc aataattgta aatccttttg atactcttga
     1081 gagattcatt ttaaaaggat aaataataaa ttcaagacaa aactgatgta ttgtccccac
     1141 ccaaatatta atatcatcat ctatactaag taaacttagg cgttccttaa tttcttcagc
     1201 agctctatta gtgtatgtga tagcaattat tctttttagt gatccatcgt gtttaattgc
     1261 ttggtaagca attttccttg taagtaccct tgttttccca ctcccaggac atgcatttac
     1321 ataaaggtta tcttcacttt ccacaacatc tttttgttct aaatttaaat catttacgat
     1381 tttttcgaag atatttgtca ttttgaaacc tcagcataaa gtatttttgt gtaattttct
     1441 atctgattga ttagatacac aacatcctca tctttaccaa agtactctaa taaagaaatt
     1501 attgctatat tcaaatcagc tgtatcactt gtttttaaac tagctgataa agctgacaat
     1561 tctgtagttt cctcgtacaa gtcgattgta tggagtagaa tttttttata aattgagaga
     1621 tctactattt cctgacatgc aaaagcaata gcattaagaa tatacttagg aattttgatt
     1681 aaaaagtcaa ttttttcacc taactcaatt gcaaaccacc ccttaccaac atgattggct
     1741 agttttaaca tcgctaaatt tctttctaaa tattgttcac tttgtatttc ttctttataa
     1801 gcagttattg tttttttatc tttgaatgcg tcatctataa ttggactgac ataagttaga
     1861 atattttctt tattcacatc taaaaactca atttcaaatg ttgtattagc ataaaaacaa
     1921 ttaacccaat tgttttcatt aaaaagggag gataattttt cttttcttgc ttgacctttc
     1981 tcttgtgcag tgcttttata caatcgatgt tctgatggga ttgcctgttc atctaaatca
     2041 gttacaatag cacactttct ctgtattcta tcatcgtcaa acaagctagc gatatattca
     2101 aacgaagtac taccaacatt aatcaaacct attccaagtt cgtctaagtt tataccaaat
     2161 aattttttta ctatttgagg aataagtatt tcttcagcat ctccttcaac taagataaca
     2221 cctttagaaa aaagcaatcc atttctttta acatctaaat atctctctac tgcgtttatt
     2281 agatttagat tgttcttttt taacttttct ttagcaaatg agttcagatt atttgctgga
     2341 ttcataacga tagatttgcc attaaacgat tttaatacat tcatccgaga tatttcagac
     2401 acttcagcta aatgtacaga atgtgtggtc atgagaatct gagtatagtt tttttgaaga
     2461 tttaatcctt cgaacagagt tttttgaata tgatgatgaa tatgagcttc tggttcttca
     2521 atcaacataa tattaagtag ttctcttgaa cgacacgctt caaattcgac tattttcaaa
     2581 gctaaataaa tcatatttag atgtcctaac cctagtaagt ctaagtcgat attttgttct
     2641 ggcttcattg aaagaaattt tgctaaagaa cccatatcat ctgacatagt tgaggaaaga
     2701 agaagattag gagaatacac tgaaccaatt atgctattta gttgccgatt aatattttcg
     2761 ccaattttcc taatttcagg aacactggta atagtctcat ttaattgctg tataattgtt
     2821 tttaatgact caacattatc tgatgatatt tttgattcaa ttgtttccat gattcttttg
     2881 actgggtttc gattgttttt catttctcta agaacatctc gaagagcgtc aacataaaca
     2941 accgaaatat gcttgaaaat atctgacatg tcaattcttg agcccaacag agcttcatcg
     3001 tcttcttcag gattactagc tatattgtca ttgagtcttc ctaccaattt atcataattt
     3061 tcattgatac agaaatttag atttgattta gatgtgtagt aaaattcata gtcggataga
     3121 cgaatagaat ttctaaattc gttaaattga tgagagtcta attcagtgga aatatcaaat
     3181 aattgctttc taatagcttt gttaggtcta ataaatagag aaatactacc tacaccttta
     3241 tctgcatttg aaatcaattc ttctaagatt gtttgacttt cagaatttaa agaaatggaa
     3301 gcacaaatct cattgtcaaa ttcttcttcg ctgattcctt caaatgtcgc agagataata
     3361 atccaatgcc cttgccaccc attttgaatt ccatgaaaaa aatctgattc ttttagtgtt
     3421 ttactactat agtaataact atcatctaat aagattctca atgcagtaag agcattggtt
     3481 ttccctgaat cattctcacc tataatagta tttgtctcat tatcgaatcg aaagatttcg
     3541 gattcataat ttctaaaatt tcgaatttga agagtagata taaacataag cagatctcct
     3601 aactatttaa ttaatatgat tatatagtaa gtttatcata aattatgtta gtaaaagttc
     3661 tcattataag ggagcgtcaa taattttgtg taaatgattc ttccctactg cagattgttt
     3721 ctaattctca atcagttggc ttagcatttg ttcgagctcg ccttcggcct gttggaaacc
     3781 tttatgactg cgacctagga atttttggtt gtaagtatca aaagacgaga ctaagaaacg
     3841 ttccatcgac tcttcatttt ggaactgttc cttgcggtgg ctgtatttct tgatttgttt
     3901 attaaaggat tcaatcaagt tcgtagaata aatactcttg cgtatggcca aggggaaatc
     3961 atagaaagtg agcaagtgat cgttcgaaag aatcgattca accacttttg gatagctggt
     4021 tttccatttt tccgcaaaag taccacgtgc ttccaacgcc acctctttag atgaggcttg
     4081 atacaccatt ttaaaatcat cacagacttc cttacgatca tcgacacgca ctttatgact
     4141 gatattacgg gaaacgtgta cacaacaatg ttgaaaacga gctttgggat agaaccgact
     4201 aatcgctcct accatcccct ttaagccatc cgtgatgaac aggaggacat ttttcaaacc
     4261 gcgctcttga aggtccaata aaatttcctc ccaaatcgtg atggattcag tcggtgcaat
     4321 cgcatagctc aatacttcct ttgatccgtc cgggcgaatg ccaactgcga tatgaatagc
     4381 ttccttggcg acggttttcc gctttaacgg aatatacgtt gcgtccatat aaatagcagc
     4441 ataacggtca tgaagctccc gcccattaaa cgccgttacc tcttctgtaa atgattttgt
     4501 tatattggac atggtttggg gcgtgtagta atgcccatac attttctcaa tcaagtctgc
     4561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaacgtgtc
     4621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcaccattgc ggtcgcgcgg
     4681 aatctggaga tgaagttccc catactcggt cttgaccgta cggtcatagg agccgttacg
     4741 agaattaccc gtgttaaaac caatgcgatc atacttttcg taatctagga aagccgttaa
     4801 ctccgttttg agaagcgtat tgactgcttt ctccaaatgg acacggaata attcattcaa
     4861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tagggaagtc
     4921 ctctcttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     4981 tttacacaaa atattttaca ctctccatta taacaattgg gttaaaaaga aaatagatag
     5041 tattctacct catatttatt tgaaagtaat atataaaaga cgttcaaatt cgaacgcctc
     5101 tcgttaacca aatattggat tcagaatcta gttttcatat ataaacagag tatgattagg
     5161 tgcaaaaatt gcacttttat tgcactttta tttttattta tagtgtttta ctgttctgta
     5221 tattgtttta actcccttga tagtatcgca ttctgtttta tacactttac gttgaaaaca
     5281 gagtgggaat aaagagccaa cataatatgg agggggaatc cctataaatc aacattcttc
     5341 aatagctaaa atgcttgatt ttcattaaaa ataagatggt tccctaccaa aagagactat
     5401 aatataagtt aatttgtttc attcttatca taaaaagaag ttctcgaaaa acgaactgtt
     5461 ttccgagaac ttttttctat ccatctaagt gctcaaatca tattcacgac ggaattctaa
     5521 ggttaccctt tcttaaacaa actttatgtt atctgcacaa ctttttgcaa ttttttcatt
     5581 gcttcttacg aaatcgttga actctcttta gcataataca aagtgtagat gaagtctggt
     5641 gctttactcg ttttggtaaa aatgactgga aatcgtttct ggttattttt ggcaaaggcg
     5701 aggtgcaggt tcgattcctg ccttctacgc taacgatgtg gctgaaggga acctccgatc
     5761 ttgaatcgaa acgatccgca gtctattgtt tcaccaatga aacaaaatca acgttcgtcc
     5821 aagcatttcc tgaagccttg ctataaaccc caatttcaca aacacctgtt gtaattggaa
     5881 tgtctttgat tgtaattttt gtccattcac ttgttttaac aaccgcagta tttagttccg
     5941 ctccaccata atttttgata tataactgat tagtatcaaa ctctccccca gacattacat
     6001 aggcactcaa tgtgtaagtt cctttgctta tgccactaac attttgataa gctgatgcct
     6061 cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc aaaaaaagtg cccgcttccg
     6121 ttttgatcgt acccttagcg gttccttgtt gaaaccaggt ttgccaattg gaaggactgg
     6181 tcgttggtcc atcgctttcg aaacttgcat ttttaactag atttatttgc gcccctgctt
     6241 gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa atcaaacatc gcttggttgt
     6301 cccattcgtt tcccatagtg gattgaacac tgagataatt catacctgct tgtgttgccc
     6361 aactcacatt tccgttgtac caaagaggtt cccaatagaa gaagcccgcc ccgcgattat
     6421 tcggtactgc ctgaatccga tcggcaagat ccagcaaata ctctgcttgt cctgtcggtg
     6481 ttggttcata tccccccgca gctgcttcgg cttcaccaaa agcatttggc atagtatcgg
     6541 cattcgctgt tgtatgccca taagctgttt caactacgat cacttcttta ccataccgtt
     6601 ggctgatcgc attcatattg gtttgaagtt cattcattga accgtgccaa tacggataat
     6661 acgacactcc aattaaatca aatgacacac ctcgactcgt gatttcatca aaccaccaag
     6721 taaatgctcc tgtatcgcca ccatcagcta agtggagcat tactggagtt gttttaggtt
     6781 gagtgtctcg aacagcttgg ataccagact tcaaaaaagc agccaatcga tcaaattctc
     6841 ccccgccttc tccccaagac ttgccataag gccagagcat tccgctattc agttcattgc
     6901 cgatctgaac catatcagga taaacgtcca gctgcttcat ttccgacaga acgttctttg
     6961 tatagtcgta caatgccgta ttcatttctt caaaggtcag accttgccat gctttaggaa
     7021 gattctgttt cccaggatca acccaaaaat cactgtagtg aaaatccagc agcaccttaa
     7081 gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt ttccagatca tttgtccctg
     7141 ctccataact ttctccatta ctattataag gatcctgcca caaacgcagt cgaacatagt
     7201 tgacaccatt attttttaaa atctctaagg catcttttga aatatcgtct tcataatatt
     7261 ttgcaccaga tttttccata tctgacagga tcgaaatatc tgctccgcga ataaattcgc
     7321 tggcagatgc ttcaacagtt tttgaaacag cccccaaagc ccctaacaca gctgcaaata
     7381 aaacagcttt acgcaaccaa attcccattt tcattatggc tcctccttta ttgaaattgg
     7441 ttcagaataa actgaacagc tttttcagga tctctcgtta atccgatata ttctgctcct
     7501 tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt ctttgatatc atcatagtaa
     7561 gaattgactt gcggtgctaa gaccaccata tcaaaatcct gcagcatttc gtaatgagaa
     7621 ccataagcac ccgccattgc actgatcggt gctttcgatt cagatgcacc tttttttaaa
     7681 gcattagcta acatcgcact tgtccctgcg ccagcacaaa gtaccaacac attgatttct
     7741 ttatttgcat cgctgataac ttgactcgct gccacaggta cttcctcacg tacatcggca
     7801 gaagcacctt cctcggttgc gatcacctct gctttttgtg cttctttctc tactaagaca
     7861 gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt caacgacgat tagaaccgct
     7921 gctagaacaa aagctaagat acccatgccc gttcctagaa ccaaaccgat cggtgctgga
     7981 gttgcccaag gcaaaacgta cataaagctg ttcatgccca atacatcgac aaaaaacttg
     8041 aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa taaaaaaata agggttcaaa
     8101 atcatgggtg ccgcaaatag caatggttcg ttgacagcga aacttactgg aataaaggaa
     8161 gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga gtaggaagat aaaaggaaca
     8221 accaatgttg ccccagttcc tcccatagtt cctacaaaat tccctaaacc caccgtcagt
     8281 actttagaag cttgttcacc ggaattgaac aattgcagat tggtttccac attggcatag
     8341 ataattgccg caattgccgg ctccacgatc gacggtccat gaacaccgac aaaccaaaac
     8401 agcgccatcg ctccccagat aatagcgatt cctaagtacc cgtcagctgc tgtaaatagc
     8461 ggctgcagca ccgtaatgat tgcttctgca aatgagattt ggaagatttg tcgaatgcct
     8521 aaatcaatta aaacggccgc aaatactgaa aaagagaaag gaaaaatatc tcggaaggtt
     8581 tgcgaaatcg ttccaggaac ttctttaggc attttaatgg taatatcttt cagcacacaa
     8641 aacttatata aattaactgt taaaaatgca gcgacaaagg aagccagtag tccctttgtc
     8701 cccatatatt ccgttgaaaa tccaccttca atcggagaga cgcttaacaa caggaaactt
     8761 accacagagg ccagcatcac agaggtggtg ttgattactt tgtttttcgg taagcgacga
     8821 ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa cgagtaaccc tacaacaccc
     8881 atcgaataat tatagacttt ccatagccat gtggagacag attcaggcca ttggtaacca
     8941 aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg aaaataaaat agctggcatc
     9001 gctgttaaaa atccgtcacg aacggctcct aaataactat tacgtgagat tttttcaaaa
     9061 aatggtttcc ctttttcgat ttgtttaatg attccatcca tagcgttctt ctcctatcct
     9121 tgattctgtc tgtacagtac aataaagtct tgaatcagat ctctcagcaa taaagtggtc
     9181 attaaatgat cctgcccatg gataaaaatg aatcccatct ccatctcttt cccagatgct
     9241 tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg ttaaattttc gtcggcttct
     9301 ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta cttctttcaa taatttcaat
     9361 aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa aaccaagcat ttgtaattcc
     9421 tctttattca tcttttcttc ctccattatc acttattaaa taatcgtttt ggtttcactg
     9481 accaacttgt accagtaagc tgattctttt gggtaacgct tctgggtttc aaaatcaaca
     9541 taaaacaagc catacctttt gttataccca tttgtccatg agaaaagatc cataagcgac
     9601 cacaaaaaat accccttcac atttaccccg gctgttatcg ccttgcttaa ggattccaaa
     9661 tagactctca aatagtcgat tctcggctga tccataataa tgccatcttc aaactgatct
     9721 ttatacccca tcccgttctc agtgatatag attttgttgt aatgtgggta atcgctttta
     9781 attcgcagca acaagtcata tagaccttct ggataaatca gccagtccca atcagttcga
     9841 ggaattcctt ctttataaat acgttctccg attcccttca ctttataaac agaggtccct
     9901 ttttcacccg taccgttatg atggattgca ttttctccat cgtaagcttt gacaaaatgg
     9961 cattggtagt gattgatccc taagtagtca ttacgcgtgg atgctttttt caattcaacg
    10021 aaatcttctt caggaaaatg atagctggcc tgatttgctt cacatatctc atcaagagca
    10081 gtcaaggttt cagttgaata atagcctaaa taggtagcat ctaataagaa acggatcgac
    10141 aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg cgtctgttgc cgcatatttc
    10201 gtctctaatg agtgaactac accaatttca cccggaagtt cgttttcttt gaaatagttc
    10261 actacacggg catgagcaac catcatatta tgaagacagg cgacgatctt tgtaaaatca
    10321 tatttgattc ctggtgggaa aacacctaac aaatattgat tggttgcaac tggatagatt
    10381 tcattaaagg tactccacac cttgacttct ttgaattcat gaaaacaaaa aatggcatag
    10441 gaaacaaatg cttctattgt ttctcgattc aaaaaatctc catggtcaaa caaacgttta
    10501 ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt ttcttttctt acattcttcg
    10561 aacacacgat gataaaaagc gattccttct ggatttggtt tcccagctcc atcaggaaaa
    10621 atgcggctcc aagcaatgga taatcttaga ccatttacac caaaacgttc acacaattca
    10681 atgtcctttg catattgatg ataaaaatca cttgccggat cgggactgaa tcgcccttgt
    10741 tcctctaaaa agtcatccca agcaacagcg cctttgccac cttccttagt ggctccttca
    10801 acctgatagg ctgctgtggc tccgccaaaa ataaaatctt ctggtaattt aagcatgtac
    10861 atctctcctc aaatctataa tcagttttcg ctctttctaa ctgattgcat tgtaaacgca
    10921 tccaaaacat ttgtcaacaa aaacaaacac aaataatcat aaaaaccaga aaacagcaat
    10981 aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt tacttttgtt cgaattagat
    11041 tgactgttgt ccgtttatat gatagattat ccttaaatta gaggtgaaga caatgttgaa
    11101 aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa aaaggaatcc ttaccgttaa
    11161 tgaaatcatc aatcaattag atgtttctga tatgaccgtt cgcagagatt tagatgaatt
    11221 agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct caaagtcttt cctacacttt
    11281 agatcaagaa ctttctcaca ctgaaaaagc cactctgcag attcaagaaa aaaaagaaat
    11341 tgctgccaca gcagcctcct tgattcacga gggagaaacg attttcttag gaccaggtac
    11401 aacaatcgaa ctattggcga cgcatttact gaacaagaaa attcggatca ttacaaataa
    11461 ttatccagtt tttgatattc ttgctcaagc agatggacca gaaattctac tgattggcgg
    11521 tgatttccgg aaaaataccg gcgcatttgt cggaccgatc accaacgaca atttaagaag
    11581 actaaagttt acaaaagcat ttatcagttc aaatggcgtt cacaacgaag cgatctctac
    11641 ttacagtacc gaagaaggag aagcacagca gatcgctcta aataattccc gtaagaaata
    11701 tctactcgtt gataataaaa aattcaatcg agatgatttt tatgtgtttt ataatttgca
    11761 tgatttcgat ctgctcatta cggatcgcca gatctcgcgg gaggttcaag agcactatgc
    11821 acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat accactatag gagcgtaact
    11881 atgaaaggga ttatatttga tttcaacgga acaatgtttt tggactctca tctgcatgaa
    11941 gctgcttggc tgcatatgat acatgatcat acaaaagatg gtttatcaga cgaggatatt
    12001 ttaaaaaata ttcatggtcg gacgaatacg gagattttga aacatttcat ttcagagcat
    12061 ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag aaagctatta tcgtatgctt
    12121 tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc ttgaagcgac tcttgaccag
    12181 ctgactgacc aacagattcc attaacgatt gccacggcta ctgtcaaaga gaatgttgct
    12241 ttttattttg atattttcga tttagcccgc tggtttgact gggacaagat tgtctatgac
    12301 gacggctctt tccctgggaa accgcaaccg gatatttttc ttaaagcggc agacaaactg
    12361 agcttagctc cggctgattg tcttgttatc gaagatgctt actcaggatt acttgctgcc
    12421 aaacgtgcgg ggatcggaac aattattgca attgatccat tcggtaaaaa caaaacagta
    12481 tttgaacagg cacagctaaa taaagatgga accatcaaag attttactgg tttttggaca
    12541 gagcacctgt ctgccacatc cgttaattct taaacgattc aactaactac caaagaaaag
    12601 caactgaaat gcacacctcc ttttataaaa aactgccagt gaaacgacat gaaagataca
    12661 ctgctatatc cttagtgaaa tatagcagtg tatctttgac tttttcattc ttttaaccat
    12721 atgacgcgtt catctctatc tataattaac attaaaaata gtgttttcac attatttctc
    12781 aaaccaatct taaaaatcat caggagtact tttttgatat acttttatta atgctgggcg
    12841 taatggttaa aagatttcca ctagttcctc tccaccaagc tgagttgaaa aatgcttatc
    12901 tgtaaacaac atagacaaag aggaaataaa cgtttccgta tctttctctg tcccaaggat
    12961 attaattaat tttcctacta ggtaagtatc attaaactta agatctgcct tcacatcaaa
    13021 agttttgata aaagctttta tcatgcgtac aattttgtct ttatcgccat aattgatttc
    13081 accactcata tcaatatagt cactaatccc tgaagaaa
//
LOCUS       DAPBBG010000069        12802 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPBBG010000069 DAPBBG010000000
VERSION     DAPBBG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.072"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..2376
                     /locus_tag="RD477_002408"
     CDS             4..2376
                     /locus_tag="RD477_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634728.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMVEHQRKTNGQ
                     PMNDKRNGRSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKKMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNESVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ELSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYHEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="RD477_002409"
     CDS             2437..3897
                     /locus_tag="RD477_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDT7634729.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAELFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     FHFETEKYKDHPVDSQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKDI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="RD477_002410"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="RD477_002410"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7634730.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSADMYDENWKQWSLEHLPIFPNQYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGEMIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPESFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQITAQNGIFDAKFDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNRRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="RD477_002411"
     CDS             6128..6277
                     /locus_tag="RD477_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634731.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="RD477_002412"
     CDS             6358..6954
                     /locus_tag="RD477_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDT7634732.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMELDRKNLRKLNEYRRILPTDKTMRSYIKALN"
     gene            6967..7866
                     /locus_tag="RD477_002413"
     CDS             6967..7866
                     /locus_tag="RD477_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDT7634733.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQINREMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="RD477_002414"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="RD477_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7634734.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKSATESRAHADQSSTSPNTTTFEQHDTMTTNNNGLNASQNPFGGQSIDISDDELP
                     F"
     gene            8376..8633
                     /locus_tag="RD477_002415"
     CDS             8376..8633
                     /locus_tag="RD477_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634735.1"
                     /translation="MAKNISLTMDLLIPVIAFIDGISLGITENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(8792..8914)
                     /locus_tag="RD477_002416"
     CDS             complement(8792..8914)
                     /locus_tag="RD477_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDT7634736.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9068..9328
                     /locus_tag="RD477_002417"
     CDS             9068..9328
                     /locus_tag="RD477_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634737.1"
                     /translation="MEHYTGLLDKIRVIKTHPLLVRFTLRTNHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            9776..9982
                     /locus_tag="RD477_002418"
     CDS             9776..9982
                     /locus_tag="RD477_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDT7634738.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9982..10233
                     /locus_tag="RD477_002419"
     CDS             9982..10233
                     /locus_tag="RD477_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634739.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            10249..10686
                     /locus_tag="RD477_002420"
     CDS             10249..10686
                     /locus_tag="RD477_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634740.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            10679..11374
                     /locus_tag="RD477_002421"
     CDS             10679..11374
                     /locus_tag="RD477_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634741.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            11755..11934
                     /locus_tag="RD477_002422"
                     /pseudo
     CDS             11755..11934
                     /locus_tag="RD477_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11996..12180
                     /locus_tag="RD477_002423"
                     /pseudo
     CDS             11996..12180
                     /locus_tag="RD477_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            12214..12645
                     /locus_tag="RD477_002424"
     CDS             12214..12645
                     /locus_tag="RD477_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7634742.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         4707 a         2154 c         2700 g         3241 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatggttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc gttcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagcta aagaaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 tatcaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctctgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagta gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt aaaaaatgaa tctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aactatcctt tagaaaccaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaggc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtaatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaacaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattgggata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcatatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttggctac aagtattcac
     1741 gaattagcgc atgctagaat gcacaaattt tcagaattag ataccgcaac taaagaacta
     1801 caggcagaaa tgacctccta tattgtgtgc aagcattttg ggatggacac gtctgagaag
     1861 gcgattcctt atattgcgac atggacgaag aatggtcaga ctttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccagcc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 catgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctgaa
     2641 ctttttttca tagacaaaga gattcaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa attttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattctcaa ccactcacag aaaaaggaag gaattatttg
     2821 aaggaagaac gaaaactgcc cgattggttg attgattatg cagaaaaaga aggattgatc
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttggat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacat tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggatttaac ctaaagatcg gtgaccctaa acagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagacattta tgaagaagtt gcaaaagaaa tgaaagttga acctgaagca
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccaccc gaaaagacgg gtattttgag atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cggccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ccaatcaata
     4141 ccactatcat gttcctaaag ataagaaaaa gcaattcaat gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaaa tgatcgcttg
     4261 gacgatcatt caacaagcag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaagg attccagcag ttaagagacg caggagagac
     4381 ctatcccaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa tcggtatgaa
     4441 tggcagcccg ctgtatagtt tactcttgca gcaaaaaggc ataccagaaa gtttttcctt
     4501 aggtagagtc caaactccaa cgctttacat gatttatcag ttgcaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaagggaa atcccaaata accgctcaaa atggcatatt
     4621 tgatgcgaag tttgatccga atgaaaccca agcgacgcaa gaagcatttg aagcttattt
     4681 aaaagaaaaa ggtgttcaag taggaaagca gccaggcacg attcttgaag tcgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagcttatg aaagctagtg ccaaagacac attggaagct atgcaaggct tatatgaagg
     4861 gaaatatcta agctatccgc gaacagacac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat catttagatg aatacaagca ttttttaaag gccgaatcaa tcccaacccc
     4981 tattcatacg ccgaatagtc ggtatgtgaa taacaagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cacttcagca
     5101 ggccatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaggttca gcagcttcag ttaaaagcaa tcggaaaagt
     5221 gccggtagat ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccaaaacc ttataccgaa ggtacgttga tcactgcaat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc
     5581 cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggggaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccactgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagcat gtacgtctga
     5881 agcctgtgat tttaagttat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gacgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaagag aacacgacgc actacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa ttgaattaga tcgataaaat cttcctggct attgtatacg
     6301 tataaactta tgttatacta tatttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtgtacaa gctgctagaa gaatttaatg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg ataaaacgta tggtagatcg tggagagctg
     6481 gatcgtgagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcgcaaaaaa aatatgaacg cggcattatt tcacatatta ccgctttaga tttgtatgat
     6601 ttgacagata tgatccctag acagatcgat ctgactgtac cttacggtta tcacgtttct
     6661 gaaaaaggat tgaaggaatt cgcagttgag ctacactata caaaagcgga atggtatgaa
     6721 ttaggtaaga ttgaaataaa aagtcgctat gggaatccta ttatagctta tgatccagaa
     6781 agaactttgt gtgacatttg gaatccctgg tataccgttg aggatgagat caaagtaaaa
     6841 gcaatcaaaa actacatgga gttggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttgc caacagacaa aacgatgcgt tcttatatta aggctttaaa ttaaaggaga
     6961 agatacatga cacctacgca gttaaaagca aaagtgaaga atatttccag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa agttcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg ctcaaagtat
     7141 ggcattgaaa atcgcacaac caaagacatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat taacaacggt tctaaacgat attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaacg agagaagccg attactatcc aggatttagc
     7321 ttacgtgtgc tggcacattt ggaaaacatg cggccagatt ttaaggtaga tgtgactaca
     7381 ggtgactcga tctatcctgc aacaatcact cacagtcata aattgatgtt tgaggatcga
     7441 acgattcctt tggaatccta tcctacagaa caaattattg ctgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcaagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaga agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatttac tatgaaaagg atatggatgt actgaaaaat
     7681 agtcacgagc taaaagatac ttggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     7741 atcacgtacg atgagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcgaaaact agcttatcaa agacaccaac agataaatag agaaatggag
     7861 agataaagat gattaataat gtgacattag taggacgctt aaccaaagat cccgatttac
     7921 gttatactgc aagtggcaca gcagtagcga cttttaccct agccgtcaac cgcaatttca
     7981 cgaatcaaaa cggcaatcga gaagctgatt ttattaactg tgtgatttgg cgcaaaccag
     8041 cagaaacgat ggcgacatta gctaaaaaag gcagtttgat cggcgttgtc ggacggattc
     8101 aaacccgaac ttatgacaac cagcaaggac aacgggtgta tgtgacagaa gttgtggccg
     8161 ataatttcca attattagaa agtaaatcag cgacagaaag ccgtgcgcac gctgatcaat
     8221 cgtctaccag tccaaacacc accacctttg aacagcatga cacaatgacg actaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgacgatg
     8341 agctaccgtt ttagttcgag aaagaaggag taagcatggc aaaaaacatt agtttgacga
     8401 tggatctatt gatacccgtt attgcattta tagacgggat ttctttgggt atcactgaaa
     8461 atcctttggt aaatagaatt gcgaaaggat cactatttct tgtattcgtg tatcttgtct
     8521 gttcttatct tttgaaaaga gcaggaaagg ataattcatt ttggcgattg actaaagtca
     8581 caatagcagc gacagctgtt atacttacgg ctattctacc gctttatatg tagggttaac
     8641 gggaaatcgt tgaacttctt gcgaaatatt gctataatga acatgaaaaa gaggagttgc
     8701 gcgaacaacc cctcattaac agcaccgatt aaagaacggt ggcagaatat tagttatttg
     8761 gaaataaccg ctgacctgct aaagtagacg gttatttctt tttgtcattt ttaagcatat
     8821 ttacaatcaa actaatcaaa gcaatggtaa acataccaaa accaagaatg gtttgaattg
     8881 tctcatatgc ggacaaaagg cttactcctt tcatagattt tagcacttac gttcataagc
     8941 accaccacct ttcagaagtc gccaccgtct tcaaacttta ctgtcttgat cattatagca
     9001 tatctaaaat cgattaaaaa ttctcaattt tagctgtttt tgctataatt ttagcgaggt
     9061 gatcgcgatg gaacattata ctggtttact ggataaaatt cgggtaatca agacacatcc
     9121 gttgcttgtg cgatttacat tacgtacaaa tcatgaaccg attaattgtg tggtggccaa
     9181 tatagaaata gcggataaat tgctgatttt ggaagaaggc aagtatacta tttcagtgac
     9241 aggtcacttc aataaacgaa atcaactagt cattgaatca atgcaaatta gaaaccctga
     9301 tcattttacg agattaatgg gcatataaat aaaagaagag ctgcagtcct aaatgaggat
     9361 tgcagctctt tttgttttac atagaaacaa gaaaaatgaa aaggcctttt taggaaggcg
     9421 caagcgcctt ctcaattgaa aaacctctct ctcactccaa ccgccaccgc cgttcgagaa
     9481 gaaaggaagt gaacgttgca cgttcactaa gataaggggt cttgcaaagc aagacaaaat
     9541 agaaaaaact tcctctcacc aaaaggttcg ttcctgttcg ctatctcttt attgatttgc
     9601 cagaccggca acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca
     9661 cgctgcacgc ccttgtcctt gtcgctttta gttgattcat tctggccaac aaatcaataa
     9721 agaaagaact aacgaaagga agtaaaacct ttcctaagag gaggcagaat caatcatgcg
     9781 aaagaaattt gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc
     9841 atatactgtc gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct attgtttgta
     9901 tcaatgtcca acatgcggga aaaaatatac cttgtcttat ggcgattata ttttgattga
     9961 tgagaaagga aagaaacgct gatgaaaaca caacacaaat tattgactgt aaaagaagtc
    10021 gttcaaattt tggtggacaa agaagtcatt tctgaaaaag tagcagaagc tttacttatt
    10081 ccgcggtact ggacgataga aaccccttta gtagaggatg caccgactat gctatttgaa
    10141 gacctagtga agattgcagg atatgaactg cattatcaag aaaacatgga aacttatgaa
    10201 aacgaatatt ggttaaccaa attagagaac taaaaaggag aaaaaaatat ggctcaaaat
    10261 tactatgacg aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg
    10321 acattcctat acaatgagac acgtgttcca aaaaaacatt ataaggaaag attgtcggta
    10381 gctgtggaag aaatgattga atcgggtgtt gaaatgaatc tcattgcgac ctactatcga
    10441 acgcttgaag aactgaaaaa gcaaaatgga caatggtttt tccaagcctt actatgcttg
    10501 gaagcaggcg tcaagccaaa cactatcaaa ccttctgaat accaagcttt agaactaacg
    10561 tatgggaaat ttgtcgaaac gaaaaaagcc aagactgttt caagtgaatg gttggactac
    10621 ttagaaaatg tcaacaagta tggcgcatgt tacatcatga aaaaggagga caacgaaaat
    10681 gaataaggtg atttatcaac taacaacgga agatgtgtca cactttttaa caaacaaaga
    10741 acatttgaat gaagtaatca gacggattca aaaagacttt agttgtatgg aagaaatgaa
    10801 gtactttatt caaagagtcg aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa
    10861 ggttacagtc agcaataatt atttagacgt gtataaacgt cgtcctgaca cattaaaaaa
    10921 aggagcggag cttctacgac tagaaacaga aaaatatatc atgaatgttt acctagaaag
    10981 gagtgaactg gtcgaaaact ttgtcagcta cacgatttgc aggaaacact attactcaga
    11041 atcagaatat tattcaattt ttgagagcat ggaattaatg gaagagctaa acagcaaaga
    11101 tgcgacggta gacgattatt accttgtatt tactaaattt ttgacgtgtg ggacggaatc
    11161 tatcgtgtta atgggaatgg attatgaact cgctgtaccc gaagtatttt tgagggaata
    11221 tgtagaagaa gtagataacc caggcgacga ctggtacacg actttaactg attttttaga
    11281 tacctacact tacgacgata cagaagaaat ccaaaatgaa cttgaccgta aacgaatgcc
    11341 atatcaacga tttgaaagga aaaaaggtca ataaccattt ataccgaaaa gtgagaatgt
    11401 gtgaaataag aacaaaaaac cttgaaatta agggaatggt tcttatttcg caccaatcgc
    11461 tccaaccgcc cccgccgctt aggtggtgcg aaataagcga gcaaagcccg aaaacctaag
    11521 agaaagaagg agatttatat gaacaaaatt attcccttat tattagtcct atttgttgga
    11581 gaaatactta tgcgacttag tgcagctgta catttaaacc tcgcagtatt ttttgtaata
    11641 ctaatgtttt gtttgatctt ttggcgattt gtattagccc tcgtaagtct actattcaag
    11701 atgattgcag ccatattttt cataggatta tttcgatgaa aggagagagt accaatgcca
    11761 attgcaatag gaaataaacg gttacccgtg acgttggatg aaaagagaca aaaagaattg
    11821 cagtaaccaa agcagaagta cggcaaaagt gaatccagga ttatgtgtat tgcgttagat
    11881 ttattgattg cgcaagaaaa agcaggattt gacataccag cactcaaaaa gtgatcgcac
    11941 tttgcgcacg tgttaatacg tgttataatt attttgtaag gaggtatagt gcacaatgcc
    12001 acttacggga aaagaaatgc ttaagttatt gaaaaaaatg gctgggttga gcgaaggcaa
    12061 gagggatttc atcatcatct ttataaggat ggtgttagaa tcactgttcc tgttcatgcc
    12121 aatcaagatc ttggccgagg acttgagcga aaaattttaa aggatgcggg attgaaataa
    12181 tctcgcattc gattaaagaa aggattgact gaaatgttaa gtgagccttt agcaaaaacc
    12241 tatcctgcta tttttagccc agaagaaggt ggtgggtact ttattgaatt tcctgatgtt
    12301 caaggtgctt acacaggaat taacgaagac gatatatctt atggaattgc tatggcacaa
    12361 gaggtgttag gtatggtttt agcagattat attgagcatg aagatctact tccagaacca
    12421 acaccaatca ataagatttc tgttgaagat gattctttta ctactttaat tcgtgtagat
    12481 gttgccaaat atttgaaaga cacagaactt gtcaaaaaaa ccttaacaat tcctcaatgg
    12541 gcggataagc taggcaaaag ggctggcata aatttttcag tccttctaac agaatcaatt
    12601 gcagataaag cagataacat tcttcgttct ggaagaaata attagaactc aaaagggtca
    12661 gaaacttaaa aatctttggc ttcttttttt actacaaaga tggcttcttt aaattatttg
    12721 cctgattgat ttaaaaaaat tgcgcaaaga ctacgagcta attaagttgc tcgtggtctt
    12781 tttttatgaa aactttttag at
//
LOCUS       DAPBBG010000070        12750 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPBBG010000070 DAPBBG010000000
VERSION     DAPBBG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.073"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            97..540
                     /locus_tag="RD477_002425"
     CDS             97..540
                     /locus_tag="RD477_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7634743.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="RD477_002426"
     CDS             complement(546..1730)
                     /locus_tag="RD477_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDT7634744.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="RD477_002427"
     CDS             1998..2696
                     /locus_tag="RD477_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDT7634745.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="RD477_002428"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="RD477_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDT7634746.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="RD477_002429"
     CDS             complement(3506..4039)
                     /locus_tag="RD477_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634747.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="RD477_002430"
     CDS             complement(4336..6717)
                     /locus_tag="RD477_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDT7634748.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="RD477_002431"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="RD477_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDT7634749.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="RD477_002432"
     CDS             7418..7957
                     /locus_tag="RD477_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDT7634750.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="RD477_002433"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="RD477_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDT7634751.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="RD477_002434"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RD477_002434"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="RD477_002435"
     CDS             9064..10230
                     /locus_tag="RD477_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDT7634752.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="RD477_002436"
     CDS             10276..11772
                     /locus_tag="RD477_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDT7634753.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="RD477_002437"
     CDS             11853..12536
                     /locus_tag="RD477_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7634754.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4278 a         2230 c         2635 g         3607 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttattcg gaagaatagg
//
LOCUS       DAPBBG010000071        12008 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPBBG010000071 DAPBBG010000000
VERSION     DAPBBG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.074"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(112..474)
                     /locus_tag="RD477_002438"
     CDS             complement(112..474)
                     /locus_tag="RD477_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7634755.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(485..1606)
                     /locus_tag="RD477_002439"
     CDS             complement(485..1606)
                     /locus_tag="RD477_002439"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDT7634756.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1622..1972)
                     /locus_tag="RD477_002440"
     CDS             complement(1622..1972)
                     /locus_tag="RD477_002440"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDT7634757.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2102..3613)
                     /locus_tag="RD477_002441"
     CDS             complement(2102..3613)
                     /locus_tag="RD477_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7634758.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3935..5287)
                     /locus_tag="RD477_002442"
     CDS             complement(3935..5287)
                     /locus_tag="RD477_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDT7634759.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5440..6516)
                     /locus_tag="RD477_002443"
     CDS             complement(5440..6516)
                     /locus_tag="RD477_002443"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDT7634760.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6712..8457
                     /locus_tag="RD477_002444"
     CDS             6712..8457
                     /locus_tag="RD477_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDT7634761.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8737..9174)
                     /locus_tag="RD477_002445"
     CDS             complement(8737..9174)
                     /locus_tag="RD477_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDT7634762.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9158..10531)
                     /locus_tag="RD477_002446"
     CDS             complement(9158..10531)
                     /locus_tag="RD477_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634763.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(10853..11341)
                     /locus_tag="RD477_002447"
     CDS             complement(10853..11341)
                     /locus_tag="RD477_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7634764.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(11363..11965)
                     /locus_tag="RD477_002448"
     CDS             complement(11363..11965)
                     /locus_tag="RD477_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7634765.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3444 a         2611 c         2169 g         3784 t
ORIGIN      
        1 atacgttcgc ttcttctagc agaaatgctg gtactagaag cagacgtatc tttttttacg
       61 cacagctggt ccttttttat atgtaaaggc tgcatggtta cttcgctata tttatttagt
      121 aacaggagtc agtgcttcga tacccacact aattttcaat gctcggtcaa cttctgccat
      181 aatgggtcga tccaaatggc agaccttttc tttcaatctt gatttatcga tcgttctgat
      241 ctgctccaac aggataacgg agtctctttc tattcctgtt ccagtagctt tgatcccaat
      301 gtgagtcgga agcttaggtt tagccatttt tgccgttatg gccgcaacaa tgatcgtcgg
      361 gctaaaatgg ttacctagat tattttgaat aaccaaaact gggcgtgttc cgccttgctc
      421 cgatcccacg actggagaaa gatcagcaaa ataaatatct cctcttttta ccataggatc
      481 ctccttattc ttgatattct cttggtatcc gctgtcctaa tccgcaagcc acttcatagt
      541 ggatcgtacc taattgatct gctatatcct gcatagtgat ttctttgtct gcatttttgc
      601 cgataagcgt cacttttgtt ccgacaggaa attcttgagg caatcggatc atcagttgat
      661 ccatacatac acgcccaatc gtttcacagt agttcccttc taccaataga gaaaaaccct
      721 gcatctttcg tggccagcca tctgcataac cgatcggtat cgttccgatc cactctgctt
      781 caggagtgat atatgtttct ccatagccga ttccttcacc tgctgggagt tttttgactt
      841 gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg ataaacttcc ggtagtgcgt
      901 gcccagaggg attaagtccg tacattgcta ctccataacg aatcatattg ccgattgttt
      961 catcatgcca taaagccgtt gcactattgc tgacatgtac atatcgagga agttcgggaa
     1021 gttttttcag cacttctata aagcgttctt tttgcagatt ccaatatgta tcatctgcct
     1081 gatctgctgt agaaaaatgg gtaaataaac cttcccattc taatacacga ctttctttga
     1141 tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa tccgatacga cccatcccag
     1201 tgtctgcttt tagatgaatc gaaagaggcg tctctagctt acttttctct aaaacagcag
     1261 ctgctgcttg caaccattcc cgtgtacctg cagttactga taaatcatga gcaacagcta
     1321 cagtcagtgc gtcgtaagga accatattca agataaggat gggctgagta aatccagctt
     1381 ctctcaactc gactccttca tctaaatttg aaacacaaaa gcccgaagct ccacctgcaa
     1441 ctgctgcctc agctgtttca atagctccat gaccgtatcc gttcgctttt actactgcaa
     1501 acaattcttt tccttgcgga agtcttgcaa cttcatttgc tacattttca gttatagctt
     1561 ttttatgtat cactgctttc gttgggcggt gccaagcgac aaccatcgtt tttccttctt
     1621 tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt tgtatgagta atactgaccc
     1681 atacattgcc ttcatgaggt gaacgcgtca caacaggctg accattttca ttttttaaaa
     1741 tctcgacatc ttgaaaggta acttttccga tcccagttcc ccaagcttta gagaaagcct
     1801 ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc aaggactgga
     1861 atagtttcat ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt tcttcgatca
     1921 gccgagtgat cctaggaagt tcgactgcat cgatcccaat tccttttatc atatgctttt
     1981 ctcttttcaa taaatttaga acactctcat tctaccaaat tataaccatt ttgccacaaa
     2041 taagtccaca aataacaaaa atgaaagttt ctcttaaaaa agagaaactt tcattttatg
     2101 tttaatcgtt atttgaacgg attacaaatc cgcgtttttt atcattgtga cgtttaccgc
     2161 catctttttg gctgttatta tttttgctat aaccgccttt tgatttgttt ccacgataat
     2221 taccgccgtc acgacgatta cggttgtttc cgccaccacg tttgccattt ttattgtatc
     2281 cttttttctg cattggcaat gggcgttctg gtgtgatctt cactggcact gcatcagctg
     2341 gatcttttgc tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa tattcttcta
     2401 ataatttatc tgctgtttgc aggtatttat ctagaccatt ttctgcaagt tttgtttcga
     2461 tttgttcaac tgcagcacct aattgacctt tgaatgcttc tttttcggtt ggtggacgta
     2521 aagtcgtcat ccgttttttc gtcaagtttt caatcacatg caagtagtcc atttcatttg
     2581 gtgtaacgaa agtcacagac ataccgcctt ttccggcacg gcctgtacgt ccgatacggt
     2641 gaacgtagct ttctggatct tgagggatat catagttgta cacatgagtg acacctgaaa
     2701 tatctaatcc acgtgcagca acgtctgtcg ctacaaggat atctaaatga ccgctcttaa
     2761 atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc gccatggatt ccttctgcac
     2821 ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac acggcgtttt gtacgtccga
     2881 agacgattgt taattcaggt gtttgaacat caaacaagcg agtcatgata tcgaattttt
     2941 cgtattcttt tgcgcggaca tagtattgat cgatcaagtc agctgtcatt tctttcgctt
     3001 tgatcttcac atgatgtgga tttttcatga atttaacgcc aatattcttg atagctggcg
     3061 gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa atgatttttt
     3121 cgatatcttc caagaagccc atattcaaca tttcatctgc ttcatccaaa actaatgttt
     3181 gaacggttcc aagtttcaat gtgtggcggt taatgtggtc aagcatacgt cctggtgtac
     3241 ccacaacgat atgtggacga tctttcaagc cacggatctg acggccaata tctgctccgc
     3301 cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa acgatataat tcttcttgtg
     3361 tttggatagc taattcacgt gttggtgcga taactaaacc ttgtaattcg tgacgatctg
     3421 gatcgatttt ttctaacatt ggtaatccaa atgcagctgt tttccctgta cctgtctgtg
     3481 cttgtccaat aacgtctttt ccagctaatg ctagtggaat cgtttctgct tggataggcg
     3541 ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa ttctggtgat aattctagtt
     3601 ctttaaattt caaatgaatc ctcctatatg gttgttaact gaggatgtag cttttttgct
     3661 accccgtcga tttgttcggt caaagtgtat tgtttctttt tgacagtggc cacgcacgtt
     3721 tttatccatt cttagcccct gtggcaccac cgaaaaatca ctttgcgaag tttaggcatt
     3781 agaaaagcca aacgggtcgc gttcagcttt atcattatta tgacatctgg cttcaacaag
     3841 ttgtttctat ggcaaaacca gtccaatttc tcttcatctt tcggcaatag gttattctaa
     3901 catagttttt cgttttctgc aagtttacag atttttattt catttgctga agttgttcta
     3961 ccacttcaaa taagcccatg ctgttgctgc cttttagcat aatactatct gttgctgcaa
     4021 gattttgttt gatagcatca atcaatttat tcttttcttt ttcaaaatag aacacaggta
     4081 gttgggggta tttttggttc aattcttctt ttagtgcatg catttgttcc ccataaagga
     4141 aaatcatggc gaatttctca ggatcgatat gttcactcat ttgcgcatgc atatttaaag
     4201 actctttccc taattccagc atatctgcta acacagcgat tctgcgtcct tttgttggca
     4261 gatttttaaa agtatctaat accagtccca tggctgttgg atttgcattg tagacatcgc
     4321 tcaaaatagc tgcaccattt cctgccgtta gccattcggt tctgttttga gtgatagaaa
     4381 catgggcgag cccttggcgt atctcaggta tggacagtcc aaagaagcgc ccgatcgtat
     4441 acgcaatcaa agcattcgtt acattataac ttccaggcag cgggatcgta aatgtttctc
     4501 cttctactac aaaatcggtt tgtgcttttc cttttgctgt cacttccgca tgaacatcgc
     4561 catttccaat accgaatgtt gtgactgttt gtactaatgt tgccgttaaa ggtgtcagca
     4621 acggttcatc tgcgggcaca acgagtaatc cgtctgatac aagtccgtca gtgatttcca
     4681 ttttggcttt agcgattcct tctcgcgacc caagattttc tatgtgtgct tcaccgatca
     4741 ttgtgattgc tgcagctttt ggttgagcca taagtgaaag ttccgtgatt tcattggcgt
     4801 gatccatgcc catttccaaa acgatctttt ctgtatcttg gggcatgtgc aaaatcgtgt
     4861 aaggaagacc aatattattg ttgtaatttc cttgcgtctt atatgttttg tatttttcag
     4921 acaagacaga cgcggttaga tctttcgtgg tggttttacc attgcttcct gtaattgcaa
     4981 cgacttctgc gcctactttt tccaagtaat aggttgccaa ttgctgcatg gctttcaaag
     5041 gatctgctac aggaataact ggaaaatttt ttggagcttg ttcaacaggc aagctccaaa
     5101 atgaagcaga tgctccgttt gtgatcgctt gctggataaa ttcatgtcca tctcgcgtac
     5161 cagcaagagg aacgaataaa cttccttcgg ttatcttccg gctgtcaaat tcgacattgg
     5221 taatctttgt cagtgcagag cattcgctgc ccacggcttg tgcaatttct tgtattgtta
     5281 gattcattga gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct aaacggcggg
     5341 ttcgcatgac tctcacttcc aagtaaattt ctctattcgt atttagaagc tagaatgaac
     5401 gcggttacgc aatcactcca gcctcttctt tatttctctt tattcatttt tttcaaaaaa
     5461 agattgacgc tgatggtatc gattcattcc taactggatc agttcttcaa tcaaatcacc
     5521 gtatttcaag cccatatttt cccataagag tgggtacata ctgaactccg taaatcctgg
     5581 catagagttt aattcattca ggaataattc atttttattt gtcaaaaaga aatcgcaccg
     5641 gctcaatccg cttccaccta acatcgtgta agctaacttc gcgtactctt gcgctttttg
     5701 ataaacttct tctggcactt cggctggaat ctgcatttcg attttattat tgatatattt
     5761 tgcttcataa tcatagaatg ctacgtcttt tacgacttca ccaggcaaag tcgtccgaac
     5821 gtcttcattt cctaatacag caacttcgat ttcgcgcgct tcaattcctt gttcaacgat
     5881 tgctcgagaa tcatactgat aggctgttgc taaagcattt tgcagctctt ctcggttttc
     5941 tgcttttgta atgccgacac tagaacccat attcgccggt ttgacaaaca tcggataaag
     6001 caaagaacct tcacattgat caaatacttt tttaggattt tctttccatt gattcttaag
     6061 tactggtaca taaggaactt gcggcacacc agcagcttgt aaaatatact tggtcatgat
     6121 tttatccatg gcacatgcac tggtcaatac gcctgcgccg acataaggca tattcaatgt
     6181 ctctaagaag ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa ctggaaaaac
     6241 gatggctcct tcttctttga tttcgcccgg attgatcact tttcctgtaa agccttcctc
     6301 tgtctgtcca cttgggtccc atgaaagatg caagacatct ttgctggcag gtttttctgt
     6361 tagtaatgga cctttgaccc attgtccttc ttttgtaata aatacgagtt gaacttggta
     6421 ataattataa taaatggcat ttaaaactga aaatgcggaa agaatggaca cttcatgctc
     6481 tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct gtattctaaa
     6541 tcgttcagtg atttactctc atattttagc acaaaacaaa aataaacgaa taacttattt
     6601 ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt tattttttgt cagctagatt
     6661 atttgtcccg ttttttataa aaagttatga taaagaaaag gagggatgat gatggcgcaa
     6721 acaaatccac gcagccgctt ggcagaaatc attcgagtgt tcatcaatta caatgtagtg
     6781 ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga agaactaggc
     6841 cccaccttca tcaagatagg acagatgctt tctgttcgag aagatttgct ttcttctgcc
     6901 ttcactcaaa cattcaaaac attacaagat agtgtaccta gcgacacatt ttcaacggtc
     6961 aaaaagacga tcgaaacaga actttctctt tcattatcag atatattcga tgatttttca
     7021 aaatcgcctt ttgcctctgc ttctatgggg caggctcatc gtgccaagct gaagaacggt
     7081 gacgcggtag tcgttaagat ccaacaccct aacatcgccg aagagatccg acttgatctt
     7141 caactatttg aacgagcgat ccctttgatc aaatacattc ctgaaactag cgtagttgat
     7201 ctaaaagggg tactccaaga agtaaaacgt tccttgatca atgaaatgga cttcttaaaa
     7261 gaaagtcaaa atggcgaaca attctatcaa aagaacaatg gctggaaaga gatccgcagc
     7321 cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga aatgtctggt
     7381 aaaaacttga atcatttaat gaacgccgaa aacaaaactg aaacttttat cactggaatt
     7441 caaaacaagc agctgaaaca agaagttgca aagcttttag ttgagaactt tatgaaacag
     7501 gtgtttgatg atggtttttt ccatgctgat cctcatccag ggaatctgct attccatgta
     7561 ctaacaaaag aagaacagac acaagctagc cgaaaaactg aaacagtcca tgaaaaagag
     7621 ttcggttcct ttgcttttcg agcctccact tctgcagaag accctgttgc gccatatacc
     7681 atcaactaca tcgattttgg tatgatgggg catctttctg caggacttag gcaaaaactc
     7741 acacaagccg ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa agcggtcctt
     7801 cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga actgacaagt
     7861 tttcttgaac agtattatga ttctcctatt gatgaaatca acttgcaaga agtattcaca
     7921 catgtcgtga cgatttgtca tcaaaataat ctgcagttcg atcgtgatat cacgcttttg
     7981 ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc tcgatcctga agtttccctc
     8041 atggaagtcg cttcgccttt tgctcaacat tattttctca ctcatctgga tgtagaagat
     8101 acattgaaac aaagcggatt agacttattg gaagggatga aggctgcgcc taagatccct
     8161 caacagctcc accatctatt agaaatgtgg acttcgggac aaggaaaagt caatttggaa
     8221 ttgaaaaagc aagacaaatt attgagcagg atcgaaagta tgatcaatcg gcttgttttc
     8281 ggtatgattt tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc agcacctgta
     8341 gaaaacgcag aggtcgtttc cttattaggg atatttacgt atgctattgc cgcattcgtc
     8401 attatttttt tggcaatcga tgcattgatc caaatgtata agaagaggaa aaagtagaaa
     8461 aaggttgtga cagaaagaat cagcttcaag aaataagaag gggttttaca taaattgttt
     8521 ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg ctacttctga aacaccgaga
     8581 ataaaaaatg tgaaaaagct gttctgcacc cagtaataag aacaatcaaa aagggaacat
     8641 ggaacacatt tatcctgttt ccctgttccc tttttgatgt attcgagcat aaataaaata
     8701 ccttcgttac ctactatctt ctgtacttgg taaaccttag tcaattgcct tttttaacgc
     8761 ttgacgaaaa gacatatgat ctttctccgt aaagggtttt ggaccctttg tccttatgcc
     8821 agcttttctt aactttccac tatgataacg ttgttctaga agcgtgccga tattcgcttc
     8881 gtcaaattct tttccagagt gatggaacac tcgcacaccc ttcggcaata caagtgaagc
     8941 taaaccataa tcttgcgtga tcaaaaagtc attcttctta accataccga cgattttata
     9001 atccgcacta tcagctccgc ggtctacgta gacgatttgg acatgattag cgtattcttt
     9061 ggtcgtataa tggtccacac tggtcacgat aaccacgtca agaccatagg attctcctaa
     9121 ttttatgaca tcttctttta ctggagaacc atctccatca atgaatagcc tcattctttt
     9181 tcgccttctt ccataaatga taaacagtta ctaatccgct cagtaaaaga ccaacattga
     9241 tccattttgc caagataagc agttgtacac tttctatcgg cactgattca tggttcagcg
     9301 tgatcaataa tagatggatt ggctctccca tttcttttgc taaaacaaac aaaagcgtca
     9361 tcaataaaaa ctggtggatc acactccctt tattttgagt agtatacttg acaacgatcc
     9421 ggcgatccat tggaatagct gtatcagttt gataatattc cttatttagc cagctgcctg
     9481 ttactacgtg ccagaaacta agcagaaaga cacctatcgc aaaaccattt ggaatcaaga
     9541 ggaccaataa agaagaactc agtccaagca attgcatagc caatggagac catgttaaac
     9601 gtttcaaaat ccatggtccg actatttttg ctgcaatcaa gcctgctgca taaggcaaat
     9661 acataaaaat cgaaagtttt tctaatccgt acagtatgcc aacataaaaa ctgctaaata
     9721 aaaaaaggaa attccctagc attccattta gaaaagtaaa cagatttagc cagcttggca
     9781 acttccattc cttcttctgg tgtcctaaaa caaacacgaa caagatgaac agcagcaaga
     9841 aaccataaat agcaagatca aaaagccttt catcgaataa caatcgggca tttctcaaaa
     9901 gaaacaataa gccaaagaat atcaaaaata aaacaagctc tcgcgtagct accagatttc
     9961 ttttgatgtc tttgaaatcc agttcgtaac gcggataatg actaaccgta tggtaggcca
    10021 tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt gcaggcaact
    10081 caatcgcttg atacaggaga aatagccaga tagctgcaaa taagtacttt ttcccatcca
    10141 tattgataaa accttgcgtc ttttcgtgaa aattgaccgt ggtattagca ggtacaagcc
    10201 atgctgcact cagccctagc aaaacacttg aaaaataaaa taaaggaaaa taaaatacgc
    10261 ctaatattcc aagcaatgaa ccagcaccac caatcagcag agcgatcatc agcaacgtgt
    10321 aactatctag accaaatcgt agactgttga ctaaaaatag tccagtcatc cgaaaggcat
    10381 aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga gtctgttagt cccaaaaata
    10441 aaagataagg aataaaaata accgtgttac ttaagataaa gggcgttgtt tgtgtaaaat
    10501 tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca gtcttataac
    10561 taatctgctc ttcgatttta gcattaaagg ggaaataaac caattttaca gaatatattc
    10621 ttgtaaaaaa aaatacagaa agaagcataa gcttctttct gcatttttac ggtacttgat
    10681 tactcaagca gacaagccct tctccacaaa agtcttgccc gacacccctg catccggcag
    10741 gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat caattaggag ctctgtaaac
    10801 cagagtcctt ctcgacccgt cgcataaatt tactttaaca cagttcctat gtttatgcga
    10861 gagctttttt cattttataa tcgaccattt gaaatccttg accgatatct gaaataagtt
    10921 cttctgtaat ctcaaatcct tcatgcttat aaacctcgat tgcttgatga ttcccctgat
    10981 tcactcgcaa aaagatctct ctttcctttg tttgcgcagc aagttcttca taccagcgaa
    11041 aaactgaaga agtcaatcct tgaccacgta ctttatttag caaatataat ttacttaaat
    11101 aaatttttcc atttatctct tcataagcag tgtaaccgac agtcttttct tcgcttttta
    11161 ataaaaagta ggacacacct tctgtcagtt ctttttgaat ctgttttttt gactgataag
    11221 ttttcagcat gtaatccact tgtttttgac caatgatcgg cacaaaagct tccggccata
    11281 cttcttgtat gactgccatc agctcatcca gttcattttc tgttactttt attttttcca
    11341 tacacccacg tcctttttta cattataaat gaccttgcgc ttgatggaat aaaaagagca
    11401 tgatcatgct atacacaccc accgaccatg gtttcaagaa aatcacaatc tgcataaatt
    11461 ccctaaaaga taatttcgtc aaaccagcta ctagacagat caagtcgtct ggcgcaaagg
    11521 gaagcagcaa ggtaacaact agtacttttt taatatcttt cgtttttctt gtcagcaatt
    11581 catcaaattt ctgatacttt tttggcgata aaataagctg gacaaaggct acgccatagt
    11641 aacggacaag caaaaatccg attgcttccc caatcactag gccaatacaa ctatataaaa
    11701 gcccaaatcc attgccaaac atcagcatgc ccgctactga ggagatccct cccggcaaaa
    11761 taggaatgat cacttggatc acttgcaaca gaacaaatat catcactgca tacttgccaa
    11821 attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga tgataagcat
    11881 aaagaatcaa cacaaagaaa aaaaacagac caataatggg catgatctga agacattttt
    11941 gtaaaactga tcctttttta gacatctata ttccccacat cttttttcat ttattaaaac
    12001 tgcctcct
//
LOCUS       DAPBBG010000072        11984 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPBBG010000072 DAPBBG010000000
VERSION     DAPBBG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.075"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..296
                     /locus_tag="RD477_002449"
     CDS             <1..296
                     /locus_tag="RD477_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7634766.1"
                     /translation="FIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQRILKR
                     GLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            300..941
                     /locus_tag="RD477_002450"
     CDS             300..941
                     /locus_tag="RD477_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634767.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            938..1855
                     /locus_tag="RD477_002451"
     CDS             938..1855
                     /locus_tag="RD477_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7634768.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            1852..2523
                     /locus_tag="RD477_002452"
     CDS             1852..2523
                     /locus_tag="RD477_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634769.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3064..3552)
                     /locus_tag="RD477_002453"
     CDS             complement(3064..3552)
                     /locus_tag="RD477_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDT7634770.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(3753..4019)
                     /locus_tag="RD477_002454"
     CDS             complement(3753..4019)
                     /locus_tag="RD477_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDT7634771.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4019..4282)
                     /locus_tag="RD477_002455"
     CDS             complement(4019..4282)
                     /locus_tag="RD477_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7634772.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            4758..4943
                     /locus_tag="RD477_002456"
     CDS             4758..4943
                     /locus_tag="RD477_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7634773.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5085..6908)
                     /locus_tag="RD477_002457"
     CDS             complement(5085..6908)
                     /locus_tag="RD477_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDT7634774.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7506..10211)
                     /locus_tag="RD477_002458"
     CDS             complement(7506..10211)
                     /locus_tag="RD477_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDT7634775.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10517..11464)
                     /locus_tag="RD477_002459"
     CDS             complement(10517..11464)
                     /locus_tag="RD477_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDT7634776.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11654..11923
                     /gene="rpsN"
                     /locus_tag="RD477_002460"
     CDS             11654..11923
                     /gene="rpsN"
                     /locus_tag="RD477_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDT7634777.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
BASE COUNT         3412 a         2500 c         2259 g         3813 t
ORIGIN      
        1 gatttatcga tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca
       61 ttttgaataa acatgttttt tacgtaaaag aatccgccta tcttcgtgat accttgcaac
      121 gcattttaaa acgcgggctg aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag
      181 gtatcctgac tcgtgcatca ttagtggata tcgtctacga tgtgatttgg ggagaagatc
      241 ccgcaactac tgatgttgca gagtcagcta ctcctgaaga atctaaggag gtctgaccta
      301 tgcagcagtt ttttcatcag cacggttcag aaattctctc aaaaggtttt gaacatcttt
      361 atatttcagg acttgctttg atcattggta tcatcattgc tgtaccgctt ggtgtcttac
      421 tgacacgttt tccaaaagct gccacgattg tgattggctt aaccagtgcc ttgcagacaa
      481 tcccttctct tgctttattg gcattgatga tcccgctatt tggcgtaggt aaattacctg
      541 cgattatcgc actctttatt tattcattat tgccgatttt acgtaatacg tacattggga
      601 tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg catggggatg actgagttcc
      661 aatccatttt ttccgttgaa ttgcccattg ctatgccaac gatcatggct ggtattcgtt
      721 tagctgccgt ttacgtgatt gcatgggcta cacttgcctc ttatatcggc gctggtggtt
      781 taggcgattt gatttttagt ggactgaata attaccaacc agatctcatt ttggcaggta
      841 cgatccctgt aacaatttta gcactattgg ctgattggct tctaggtctc ttggaacacc
      901 ggctgacacc gatcgcactt agagaggaga atgaagaatg aaacgtatac taaaaatgac
      961 catggttctt ttctctcttc ttatacttgc gagttgttcc ttccctggtc ttgccagcaa
     1021 tacagatgaa gacaccattt cgatcacagg tggaatcact tcagaagcac agatcttagc
     1081 aagtctagtt gccggaatgg ttgaacatta tacagataaa aataccgcaa tcatcaacaa
     1141 tctggctact acaactatca accatcaagc gatgatgaac ggagatgctt caatttcagc
     1201 ggctcgctac acaggaacag atttaacgac tacattgaac cttcctcctg aaaaagatcc
     1261 gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa agatacggtc aaacgtggtt
     1321 cccgtcctat ggttttgaaa atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa
     1381 ataccatcta tctaaagtca gcgatctgaa aaatgtagcg gatgaattag tagctggtgt
     1441 agatacttct tggatcaatc gtaaaggaga cggttatgac ggcttccaag aaacttatgg
     1501 cttctccttc aattcgatat tacctatgca gattggactt gtttatgacg cagtcgaagc
     1561 aggaaaaatg gatattgttc ttggctactc tacggatgga cggatcgcca gttatgatct
     1621 agtcatgttg gaagatgatc gtcaattctt cccaccatat gatgctgctc cagttgtaga
     1681 caacaagata ttagctgata cgcctcattt aaaagaagcc ttaaataagt taggtcatac
     1741 catcagtacg gaaaaaatgc aggaattgaa ctacgaagca gataataatc tagtggaacc
     1801 atcagtagta gcacaacgtt tcttacaaga aaaccactat tttgaaggga agtgaagcaa
     1861 ttggataaaa tgaatgtctt tcaacaattt ttctattatt tccaggaaaa tggctcctat
     1921 atctttgcac aatttatccg tcacttcctg atttctatct atggtgtcct ctttgcagca
     1981 gttgtcggga ttccggttgg gatcatgatt tctaggagac gtaaattagc aaactgggtg
     2041 attcgtttgg cgaatatcat ccagacgatc ccttctctag ccatgatctc cattttaatc
     2101 attggattag gcctgggagt caatgtcgtg attgttactg tatttttgta ttccctctta
     2161 ccgatcatca agaatacgta taccgggatg atccaagtag acaaaaacat ccttgatgtc
     2221 ggcaaaggca tggggatgac tgctagacaa cgactattca tggtagagtt accactctct
     2281 gtttctgtta tcatggcagg cataagaaac gcgttagttg ttgccatcgg gataaccgcg
     2341 atcggtgcat tcgtcggtgc tggtggtctt ggagatatca ttatccgagg aaccaatgca
     2401 acagagggca catcgattat tttagcaggt gcattgccaa ctgcattaat ggcgatcatc
     2461 acggactggc ttttaggtat tttggaaaga cggctagatc cagccagccg tacatcacgc
     2521 tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa aggctttttt
     2581 agttctgtca tttttaaggc aataatccac tctgattctt ccctccttat gggcaaagag
     2641 ttgcttttct tagctattta tccattccgt atactccaac ttcatactgc caacgggttc
     2701 atatctgcat ccaccgacta agtttttttc ttatccgcta aggcattttg ccaatccatt
     2761 ttcccatcac tcgtcatgga attcatacca catgatctct aaatactcgt ctgcttgttc
     2821 tttgaattgg gcatcaattc ttacatcctt tggcgtggct ttcatttaag ccttttccat
     2881 cagtcctcga ttgttcgatg ccttcagctg catcatcaaa actggtgact gccgtatcgg
     2941 taacttcgat catcttttat acaatcggga acttgccatc ctcatccttg gctaaaaaca
     3001 tatctagcca ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac cggtacacga
     3061 ttcttatgga ctgcatccgt cgcaactaga cttggcgata cgatgatcct tcaccagaag
     3121 agcacccccg taaaccaatt gatcgatata ttgccaataa gaaaagacat tggcgtaaaa
     3181 gatatcgatc cctcgtgatg cattgccgtt gatactgcta ttcatggccg aaagcgccac
     3241 gactttaact tcttccgttt gagtgacatt catgttcggc aatgtcgctt tggatacccg
     3301 ctctgctaaa ggaacgacac tggtattgta tttcgtatct gtatcttttc caaaagacca
     3361 tgccaacgaa tcctctggga accaataaga cgactccggt tgattgtcca ttgctttttt
     3421 caacatactt ttggctgttt ctcgataaac gacatttcca attgtcttcg cctcactttg
     3481 gcgataatcg atggtcgttg ccactgccaa cactaacaac gatgctccaa ttttactgat
     3541 tttccttgtc acacttcttc ctccttgttt tacataaacc cgtcaacatt ccattcctaa
     3601 actcaaacaa tgacccttca aaggtggaga tataggaaca aattaaagaa atcatgcaat
     3661 aaatccaaga aggttggttt tagaacaaaa aaagagtctc ctttttgggg agactctttg
     3721 ttaactatct aaaaaggttg gctcttctca cttcactcat aatgtgtcca ggcagaataa
     3781 atttctacaa ctttattttc ctcatcaact ttgtaaacca ctcgatgctg tctattcagc
     3841 ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt caaaagactg cgtgggcata
     3901 tatggatctt ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa attcgtttgc
     3961 ttgatttttc ttaaatccac tttcgcagag tttttgattg cgactgtata gttactcatc
     4021 aaagagcttc ccaatcaata tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt
     4081 ctcgtacttt atctaaagta cctgtttgct caagaaaaag ggtttcttga atcgctctcc
     4141 agtcttccag cccaatcaat acagcattgt ttccactacg atcactaata atctcgattt
     4201 cagtatgatt ttcatttacc tctttcaata actgataaaa atttttacga gcagtgctag
     4261 gattcatcgt tgtattagcc attgtctctc ctcctttttc tgatataatt atagcgtact
     4321 tgattaagta cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact tttcctcttt
     4381 tttacataga gaaaaagacc cctttttagg gaggatcttt gtccacgctc acttcttact
     4441 cctttacgac tagcggtagc caaacgtaaa gtaattggta acaacgtatc atctttagca
     4501 aaggtcgaat ccaaagcctg tgagtctatg caacaacacc aatacacgaa ggttcctcgt
     4561 tgcagtgtga cttgattgat atcttgaata acctgaggat acgcacgaaa gatttgtaac
     4621 ggttattcaa aactgatttt ggccgtcaag tcttctactg cctcaaaaat gattgcctct
     4681 ttcgtctcct gtcattcatg ttcactatcg atttgatact tgcttagacc gttgggtttg
     4741 tgaatcacca attaaaaatg gcactgagtt atggtaacaa aaaatggtca cttgacgagg
     4801 ctgagattcc tacttatgaa gacacatggc acttaagagt cgaggtaaag gaagaagctg
     4861 aaaatatagg aggctgtaca ttatttggca aaggattcgg aaattatgat caagacatca
     4921 aaatcaaaat gttttacagc taaataggaa gaataataac atttcatacg ctgttcccat
     4981 ctttaatggc ttatagaggg cacctttagc tgagaataag acaaaacaga ccctctagct
     5041 tcaattaaga gctagagggt ctgtttttga ttttaaaact tggtttattt cttcaaatgg
     5101 tagctatagg tgctgacaac gatattttca cgccaagaca atcgcaatct taaccgcaaa
     5161 cttgtttggt tatggagaat attctgccag cgtctgtttg gtacaaattg aggaatcaac
     5221 acagtagttg tatagttttg tttcgctgca tttttactta cgatatctac ataacggata
     5281 attggatttt caatcgagcg ataagaagag tggacgattg aaaaacggac atctgggaaa
     5341 tgctttttaa attctgcttc gatttctttt tctttttcga catcttcgtc tagcgaaaca
     5401 tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc
     5461 cgtgtcacat tcccgactaa tacaagaacg gtattcccgt cataatcatg tagttttgca
     5521 tcattttcta aacgaagctg ttctgctact tttttataat gatcatggat cttataaaaa
     5581 atgaacataa cgattggcat aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca
     5641 aacaaaatcg caatgattgc atatgagatg aacgcacccg taatattggc aatcgatttg
     5701 cttaaccatc tttttccttc ttttttccat ttgacgacca tccctgtttg agataaagca
     5761 aatggaatga acacgccgat agagtataac ggaatcaagc gttctgttga gccatggaaa
     5821 ataaacagca atacgatcga acctagcgct agtgtgataa tcccattgga atagcctagt
     5881 cgatccccac gatcttgata catgtgaggc atgaatttat cttttgccag attgtatgca
     5941 agaactggaa aagcagaaaa accggtgtta gcagcaactg ctaaaatcag cgctgtagca
     6001 aattgaagga cgtaatacaa aataccatgt ccaaagacag ctttaccaat ttgagacaaa
     6061 acagtgactt ctttttcagg aacgatccca taccaatagt tgataaatgt gatccccacg
     6121 aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt ttttgctctt
     6181 ggcttcttaa agaatggtac agcattacta atcgcctcca ccccagttaa agaggaagaa
     6241 ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac ccggcacagc cgctcctgga
     6301 agtgcagtag cttgcaatgg cacagcacct gtcacgatat tatataaacc aacaacgatc
     6361 agtaacgaga tcacaaggat aaatgtgtac accggaaata ataggaacga tgcagattcc
     6421 ctcagcccac gaagattcag catcatcagt agcaaaacga ttgtcacaga aatagcgact
     6481 tgatgaccgt agagtgctgg caccgctgat gtgatcgctt cagcacctgc tgagacagat
     6541 actgctactg tcaacatgta gtcgatcaat aaagagccac cggaaatcaa gccagcattc
     6601 tttccaagat tctcactgct gacgacatat gctcccccgc catgaggata agcatggatg
     6661 atttgacgat atgatagcgt taatgaaatc aataaaataa taacaaaagc ggcaatgggt
     6721 agcgaatacc agatggctgc agcagacaaa gcgactaaaa cgacaacgat ttgttccgta
     6781 ccatatgcaa tagatgataa cgcatcagac gacaataatg ccaaagcagc aaatcgggtt
     6841 aatttgtgct catcattttc agcagatttt aaaggcttac ctactaacaa cctttttaag
     6901 taatccaatt tctaaactcc tttacacaca attttacttc aagttttata gtcgaaaatt
     6961 tatagtaaac aagatgttat agtacgctca ctgaccttaa aagtcaataa actttaagaa
     7021 aataattgga aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta atacagcaaa
     7081 aatctaacat ttttttatgg gctttttctt tgaattctat aataaaagga tagaacttga
     7141 cttagaaaat caagttctat cctttttgat atcttaactt ttggaagaga gatgtaaggc
     7201 tgctttctac ccatcattct tactactcga agcatagcgc agttgtcaca acctcttgat
     7261 aaacggtgtt taaaagcttg catctgcggt aataagctca accaaattaa aaacataaga
     7321 agctgttttt atttggtcgt tcttattact tgatgcagag cagctttttt acaacctctt
     7381 ggtacacggt gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg caaaatatga
     7441 ggaactattt ttccattttt ttctctaata ctcaggtcaa aacactgatt tcacaacctc
     7501 tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt cattttttct
     7561 tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacaa accgatcatg
     7621 atccagattg ctttcgtagc atttggccaa taaattcctc ctgcagattc ccgtaacaag
     7681 tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt ccctgagact
     7741 tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat aatagcgatc
     7801 cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat gaacgctaag
     7861 gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac tcctagtaag
     7921 aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc
     7981 atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt tcctaaatag
     8041 tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca tagtgcagta
     8101 taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt ggtttgtagc
     8161 tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa taatttgatg
     8221 acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc tttttggata
     8281 ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt cacagctgtt
     8341 tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc attttttagt
     8401 gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg aatgatcggt
     8461 aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt atccatattt
     8521 ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat ttctttttga
     8581 tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgagct gagaagcgaa
     8641 tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg
     8701 acttggtcta gtagagaagt gacagtggaa ctgatttgac ctgtattgat accagcaatg
     8761 atgccattga tattattaac aacggcatca atttgatcta gcaattcatc aatagccttc
     8821 gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag attgcttagc
     8881 aagggtttta attgatttag ctgatcaata attggctgta atttcgtatt tccagcactt
     8941 gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg ttgagatatt
     9001 ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc agtcagctta
     9061 ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt tgccacttgt
     9121 ccaattgact tcaatgtaac atctacactg tttgtgatgc ttggtatcgc atctttcagc
     9181 tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc taaatttttc
     9241 acatctggca agatatcttg tacttgttgg atgatcgtca agccttgttt tgcttcatcg
     9301 attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc
     9361 ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat ttttgcttga
     9421 tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat ctcatctact
     9481 ttagggatgt aatccacgaa ttcatttgct ttattcagtt tttccttgat ttcaggtagt
     9541 tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa attttcatct
     9601 gttgataaaa tcatatcttt aactttattg atacttacta ggttcgtatc gatgtcgtaa
     9661 cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa ttcatttgtg
     9721 atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat cgcattgatt
     9781 ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt aaaactcagc
     9841 agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc ggaacggact
     9901 ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt atttttatga
     9961 agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc tgctggcttg
    10021 tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc ccataatgct
    10081 ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt
    10141 gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag cgtatttttg
    10201 attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta ccatgtattt
    10261 catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata tcacggcaat
    10321 tgtctcattg taatctctta catattgaga aataattaag gattacagcc tttactttat
    10381 catgaaataa aagggaaaga taattttatg ccaaagtttt tatctattat acgtatacgc
    10441 aaaaaagcta aaacattttt tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt
    10501 tttattcctg tatttcttat ttttcgaatg acaggatgat tttcccagca ggaggatgtc
    10561 cttcaactag ctgatgtgct tggatgacat gcttaagtga agcagacaga ttcattgaaa
    10621 taaaaacatg aaaggctcct ttttggtagg ctttgattat gccagcaaag gcttctgcat
    10681 ctagatattc ctttcttgga acaaacgatt cataaaagcc ttcctttttt tgccgcaaat
    10741 ctagatctgg gaggtcattt aatgcaacat acctgccgcc tggtttcatg atctggatgc
    10801 cggtttctcc tttgatactt cctttcgtcg catcgattac aaggtctgcc tgatcagcaa
    10861 attgcagacc tggatttgtt ttatcataag cagcaaatgc tgaagctcct aattttttta
    10921 ccttttcctc atttttactg gatgcactgg ttaagattcg gattcccttt tcatggagaa
    10981 gctggatcag acttgacccg accgcacctg atgctcctag tatcattaca gtatctgtcg
    11041 gctggatttc cgtcaaatgg ttgattagat tataagccgt aattcctggc gtgaccattc
    11101 cggctgcttc ttcccaactc attttatctg gaatttttgc tacttttgct gatggcaaca
    11161 cgacttcttc tccgtaagtc ccgccgactg catggacagc tactcgatca cctacatgaa
    11221 cagttgtgac atctgaacca atctctgtca caataccagc gccatcatta cctggtacat
    11281 aagggaattt caatgttcgt atctctttca tttctcctaa acgcaaggca acatcataag
    11341 gattcacact gaatgctttt atttctacac gaacatgtcc agaaccgact tctctgggat
    11401 gagcatcgat ttcttcaaaa acatcttgtg cttctccgta accgttaatt gcaaatcttc
    11461 gcatggtcac catctccctt tttctttaag tttacgttta tgtctttatt ttgacaaaaa
    11521 aaatgtcttt tcgttttggc gcatgattca aaacaaaata gttgtctttt ttcgcgtaac
    11581 gtgttaaatt atttatctaa aagcgtaaag attacgattt acgctaataa aaaataattg
    11641 gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac
    11701 gattgaacgc tatgctgctt tacgccagga attaaaagcg caaaaagatt acgaaggctt
    11761 gcgtaaactt cctttagatg ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg
    11821 acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc
    11881 acataaaggt ttgattccag gagtaaaaaa agccagctgg taatccaagt ctggctgtaa
    11941 tgtaaaggag gagggcgcat ggaccactcc ttggatcatc aaaa
//
LOCUS       DAPBBG010000073        11369 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPBBG010000073 DAPBBG010000000
VERSION     DAPBBG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.076"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(58..285)
                     /locus_tag="RD477_002461"
     CDS             complement(58..285)
                     /locus_tag="RD477_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7634778.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(388..2976)
                     /locus_tag="RD477_002462"
     CDS             complement(388..2976)
                     /locus_tag="RD477_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDT7634779.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(2996..3655)
                     /locus_tag="RD477_002463"
     CDS             complement(2996..3655)
                     /locus_tag="RD477_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7634780.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="RD477_002464"
     CDS             complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="RD477_002464"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDT7634781.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4428..5294)
                     /locus_tag="RD477_002465"
     CDS             complement(4428..5294)
                     /locus_tag="RD477_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7634782.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5403..6044
                     /locus_tag="RD477_002466"
     CDS             5403..6044
                     /locus_tag="RD477_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDT7634783.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="RD477_002467"
     CDS             complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="RD477_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDT7634784.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(7485..8384)
                     /locus_tag="RD477_002468"
     CDS             complement(7485..8384)
                     /locus_tag="RD477_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7634785.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(8386..9183)
                     /locus_tag="RD477_002469"
     CDS             complement(8386..9183)
                     /locus_tag="RD477_002469"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDT7634786.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(9161..9865)
                     /locus_tag="RD477_002470"
     CDS             complement(9161..9865)
                     /locus_tag="RD477_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDT7634787.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            9996..10571
                     /locus_tag="RD477_002471"
     CDS             9996..10571
                     /locus_tag="RD477_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDT7634788.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            10689..11366
                     /locus_tag="RD477_002472"
     CDS             10689..11366
                     /locus_tag="RD477_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDT7634789.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         3235 a         2471 c         2040 g         3623 t
ORIGIN      
        1 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
       61 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
      121 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
      181 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
      241 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
      301 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
      361 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
      421 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
      481 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
      541 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
      601 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
      661 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
      721 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
      781 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
      841 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
      901 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
      961 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     1021 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     1081 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     1141 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     1201 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     1261 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     1321 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     1381 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     1441 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     1501 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     1561 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     1621 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     1681 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     1741 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     1801 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     1861 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     1921 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     1981 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     2041 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     2101 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     2161 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     2221 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     2281 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     2341 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     2401 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     2461 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     2521 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     2581 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     2641 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     2701 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     2761 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     2821 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     2881 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     2941 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     3001 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     3061 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     3121 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     3181 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat
     3241 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     3301 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     3361 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     3421 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     3481 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     3541 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     3601 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     3661 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     3721 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     3781 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     3841 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     3901 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     3961 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     4021 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     4081 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     4141 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     4201 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     4261 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     4321 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     4381 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     4441 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     4501 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     4561 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     4621 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     4681 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     4741 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     4801 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     4861 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     4921 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     4981 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     5041 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     5101 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     5161 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
     5221 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
     5281 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
     5341 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
     5401 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
     5461 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
     5521 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
     5581 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
     5641 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
     5701 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
     5761 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
     5821 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
     5881 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
     5941 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
     6001 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
     6061 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
     6121 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
     6181 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
     6241 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
     6301 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
     6361 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
     6421 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
     6481 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
     6541 gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag
     6601 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
     6661 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
     6721 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
     6781 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
     6841 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
     6901 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
     6961 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
     7021 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
     7081 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
     7141 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
     7201 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
     7261 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
     7321 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
     7381 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
     7441 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
     7501 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
     7561 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
     7621 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
     7681 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
     7741 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
     7801 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
     7861 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
     7921 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc
     7981 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
     8041 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
     8101 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
     8161 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
     8221 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
     8281 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
     8341 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
     8401 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
     8461 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
     8521 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
     8581 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
     8641 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
     8701 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
     8761 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
     8821 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
     8881 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
     8941 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
     9001 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
     9061 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
     9121 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
     9181 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
     9241 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
     9301 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
     9361 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
     9421 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
     9481 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
     9541 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
     9601 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
     9661 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
     9721 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
     9781 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
     9841 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
     9901 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
     9961 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    10021 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    10081 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    10141 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    10201 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    10261 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    10321 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat
    10381 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    10441 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    10501 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    10561 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    10621 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    10681 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    10741 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    10801 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    10861 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    10921 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    10981 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    11041 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    11101 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    11161 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    11221 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    11281 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    11341 gccatttaca cttgctttct aattaaaaa
//
LOCUS       DAPBBG010000074        11327 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPBBG010000074 DAPBBG010000000
VERSION     DAPBBG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.077"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..1044)
                     /locus_tag="RD477_002473"
     CDS             complement(25..1044)
                     /locus_tag="RD477_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDT7634790.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(1217..2122)
                     /locus_tag="RD477_002474"
     CDS             complement(1217..2122)
                     /locus_tag="RD477_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7634791.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(2181..2726)
                     /gene="nusG"
                     /locus_tag="RD477_002475"
     CDS             complement(2181..2726)
                     /gene="nusG"
                     /locus_tag="RD477_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDT7634792.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(2827..2997)
                     /gene="secE"
                     /locus_tag="RD477_002476"
     CDS             complement(2827..2997)
                     /gene="secE"
                     /locus_tag="RD477_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDT7634793.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(3021..3173)
                     /gene="rpmG"
                     /locus_tag="RD477_002477"
     CDS             complement(3021..3173)
                     /gene="rpmG"
                     /locus_tag="RD477_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7634794.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            3335..3979
                     /locus_tag="RD477_002478"
     CDS             3335..3979
                     /locus_tag="RD477_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7634795.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(4035..4538)
                     /locus_tag="RD477_002479"
     CDS             complement(4035..4538)
                     /locus_tag="RD477_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDT7634796.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(4551..4597)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5135..6100
                     /locus_tag="RD477_002480"
     CDS             5135..6100
                     /locus_tag="RD477_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDT7634797.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(6199..7371)
                     /locus_tag="RD477_002481"
     CDS             complement(6199..7371)
                     /locus_tag="RD477_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634798.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(7358..8503)
                     /locus_tag="RD477_002482"
     CDS             complement(7358..8503)
                     /locus_tag="RD477_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7634799.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(8517..9449)
                     /locus_tag="RD477_002483"
     CDS             complement(8517..9449)
                     /locus_tag="RD477_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634800.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            9566..10645
                     /locus_tag="RD477_002484"
     CDS             9566..10645
                     /locus_tag="RD477_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7634801.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            10642..11271
                     /locus_tag="RD477_002485"
     CDS             10642..11271
                     /locus_tag="RD477_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634802.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
BASE COUNT         3440 a         2376 c         1973 g         3538 t
ORIGIN      
        1 aattgccctt ctcttcgttt tttattaatc catcaatttc gtatttttta cttcatcgat
       61 cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct ttgttcaggt gttcaaaaac
      121 agatgttact ccttctgccc cacctagatt caacccataa atcactggtc ggccaatcgc
      181 aactaagtca gcaccacttg ccagagcttt gaagacatgc tctcctcgac ggacaccaga
      241 gtcgaaaatc actggtacac gtttgttgac tacttcggca atttttggca atacttcaaa
      301 ggacgcagga cctccatcta attgacgtcc accatgattg gatacccaga taccatctgc
      361 ccctgcagag atagcgatta ctgcatcttc tggcgattgt attcctttta cgataactgg
      421 aagatgggtg atttctttga ttgttttgat gtcatctggt gtcaagcctt gttttgcagc
      481 tgcataaatt tctgcgattc cttttccttc gccattacca cttgcacttt gttcgctata
      541 cgcagctaag ttaggcattg gtaatggaaa ttggaattga ttgatgacgt cttcttcacg
      601 gtaaccaccg agcgttgaat ctgccgttaa aatgatagct ttagcccctg cttctactgc
      661 tttgttcaaa atgaactcat tgaatctgtc gtctttactc atatataatt ggaaaaactg
      721 cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc gtatttgcat aagtgctgat
      781 ggaaaagatt gatccagcag cagctactcc tttagctgta tcagcttccc ctttttcatg
      841 tgccaatcct tgggccgctg atggcgcttg gataatcggt gtatctaatt caattccgaa
      901 aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat atccgtggca tgattttctt
      961 tgtattaaaa gaagtcgtat tttctttcat cgtccactca tcttcagaac caccgcgaat
     1021 atagccaaaa gctcctgctt ccatgcgctc ttttacacga ccttctagtt aggcaatgtt
     1081 tacaatatcg attggatgtt cttcattact tgtatgataa gtcatagtag atccttcttt
     1141 catcttcttg attacatata aatcatacta aacttacaaa aagtaagcaa acatttactc
     1201 tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga tctacttctt
     1261 ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt tcctctattt
     1321 catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac ggtactgttc
     1381 catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga gtggttatag
     1441 gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca gcaggcccct
     1501 ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta aaaggcgcgc
     1561 caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac gttcctaaat
     1621 agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta tctatgccgt
     1681 agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc cactcgttat
     1741 ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca gacaaatctc
     1801 ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt actgcttgct
     1861 gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg ctgtagccgt
     1921 ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct gccgtttttt
     1981 ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt aacgcaaaca
     2041 aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg attggttctg
     2101 tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca atcatatgat
     2161 cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc aagttctgta
     2221 ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc tccgtcaact
     2281 tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac gacttcgcca
     2341 acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa gatatgattg
     2401 acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc aacgaatcct
     2461 gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat catttcaaca
     2521 agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt tttgacttca
     2581 gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt catactttgc
     2641 gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt atgcaagaca
     2701 taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa aataaaaaaa
     2761 cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta cgttgtggct
     2821 agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca ttacgtaaaa
     2881 catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac ggagttgttt
     2941 tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa atttcatgag
     3001 acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg attacaatat
     3061 ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac tgattttgtg
     3121 tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc catttacttc
     3181 tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac tttttacttg
     3241 tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga ttaatttgaa
     3301 attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac ttgtatataa
     3361 gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat taaaaatttt
     3421 agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata ttttccgtgg
     3481 aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata tgacgcttcc
     3541 tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt ctttcttcaa
     3601 agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg aaaatcattt
     3661 ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt ggagcgtaga
     3721 acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt tggctgctat
     3781 tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg atatcgaaaa
     3841 agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag cgaaagtatg
     3901 tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa ttgaaaattt
     3961 gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct gctttctttt
     4021 ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa ataatacatc
     4081 aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc ataggttgtc
     4141 caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat gctgatggta
     4201 aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt ttgcaataaa
     4261 gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc catgatacca
     4321 aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag caatttacgg
     4381 ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg ccctgaggat
     4441 acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc atttacaacg
     4501 attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct tttctcctag
     4561 tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa taattataac
     4621 agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa gcttaaacaa
     4681 tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg ggtcataaga
     4741 attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg aaacttaaat
     4801 aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc tagcaagcag
     4861 ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag taatggatca
     4921 tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt ttcctagact
     4981 tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa caagtttctt
     5041 tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca ggagtattaa
     5101 aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc atatattgat
     5161 caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt taccggtatt
     5221 aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac acgatgctga
     5281 gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag aacagaaaac
     5341 aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac atcaggtact
     5401 tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag caggttctgg
     5461 aaatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac ttcagctgat
     5521 tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa aaataagtga
     5581 aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta tcgtccatgc
     5641 gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat cattttcgta
     5701 tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc tggtagatgc
     5761 aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga agcatccttt
     5821 ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag tcattgattt
     5881 tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt tacttataca
     5941 gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat tacgtatcgt
     6001 ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac gaccttttga
     6061 tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt tttgtcagaa
     6121 gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt ttgtcacagt
     6181 ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat agtttctttc
     6241 tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta agacacaacc
     6301 attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac tgatcaagtt
     6361 attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa ccatttctgg
     6421 caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga taaaaatatc
     6481 tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag ccgaaaagct
     6541 aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg ttatatccca
     6601 cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca acagcagcga
     6661 gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa tgggcgcact
     6721 ctggtttcgt ttactgatct ctttgcgatt aaacgctaga ttgatgacgc tgaaaccgct
     6781 aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg tcaacagctt
     6841 tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg agagtgccat
     6901 ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca gaatcagtgt
     6961 ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat ctgggcgact
     7021 ctgtatcttg atcgtcgcca tctttcctac ttcgacttgt tcggtaaagt ctgcaggcag
     7081 atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt tttgtgaggt
     7141 tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc catcgactac
     7201 aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg tattttcaac
     7261 atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac ctatcagtaa
     7321 attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca taacgcaccc
     7381 ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc aaccatagaa
     7441 gattggtata aaaactagcc aaatcagtgt agaaataaag tttatacaaa ctttcgctga
     7501 tcaagttgac gagatttaat tgaccaacaa tcggaatatg aagatcaatc caatacttca
     7561 cttgttccga ccccatcata ccagcaaaaa agctcatcgc aatcgtcact gaaatcaaga
     7621 tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt accaaccaat gtacctaaca
     7681 aaatcgcaca caacgacccc agagcggtaa caatcatcaa ccattgccaa cgttcaccaa
     7741 agtctacttg atagataaaa cgaaagacag ctaacataat ataaatctct gtcaaaacaa
     7801 tcgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
     7861 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
     7921 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
     7981 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
     8041 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
     8101 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
     8161 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
     8221 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
     8281 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
     8341 caattccagt attaatcgtt tcaagattgc caccttgatc aatctcgcca aaggccgcca
     8401 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
     8461 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
     8521 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
     8581 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
     8641 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
     8701 gctattgttc gctgaatcgc tgacaacaca ttttctggga gtaatgggat gtcgatcgtc
     8761 aaacgatccg ccgtccgtac catatctttt agagattctt tggttccttt agcaatcacc
     8821 tgtccttgat ccatgatcac gatctggtcg caaagaattt ctacttcttc catgtaatgt
     8881 gttgtataga caatggttgc tccttgtcga ttcagctcct tgatactttc taaaatttta
     8941 ttccgacttt gggggtctac agctactgtc ggctcatcta aaaaaattaa ccgcggcttg
     9001 tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc caccactaag ttctcctgga
     9061 cgaaacgaaa catatttttc taatcccact aattgaatca cctcttcaat caaggctttc
     9121 cttttctctt tttcggaaac atataaaccg caaaaataat cgatattttc ttgcacactt
     9181 agttcgtcaa agaccgcaac gtcttgagga acgaccccaa tcattgcctt gacagcataa
     9241 ttttcaggag tcattgttgt cccgaacaat ttaatggttc ctttgtcata atttaacaaa
     9301 gaaagcatac agttgatggc agtcgatttc ccactgccgt ttggacctaa cagacctaag
     9361 acttctcctt cttgtacttg cagatcaaaa tgattcaatg ctacaaaatc tccatagcgt
     9421 ttgactaatt tatcaatttc gataatcatc atgaaaaact ccttccaaat ttcttgttga
     9481 tctgactata ataaagtgca agaaaagaaa ctagtgacga ttggctaaac cacacatgac
     9541 atttgtcaca atgaaggagt ctgctatgca taaatctcgg ctactcaacg aaactagctt
     9601 tctactggtt atctcattgc ttttgctggt aagtcaagga ttcgttgcta tgcaagttat
     9661 ctatttattg atcgccgttt tattcactgg tgtcagttat ttactgccta caaagccaaa
     9721 ttggattttc caagttattc tatttctttt atgtgccttt ttacctgatc ttgtcctgtt
     9781 tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc caccttgatt acttgttttt
     9841 gttaggtttt ttgttgattt cgacggttgg taatcatccc cccatcaagg tcgcctttgt
     9901 cgtaattttg ctgttttttt ccttttactt acgaaaaaaa gatcaacgaa cgaccgatct
     9961 tgagaaaaag tacattgtct ccaaagatac tcattgggaa aaacaacaag agttatcgga
    10021 acaaaataaa gcattggccc tgtcacaaga aacacaatta gccttgcaaa ttgtggaaga
    10081 acggaaccga attgcccgag acatccatga caatgtcggt catctgctgt caagtgcgat
    10141 tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca aaactaaaag aaccattaga
    10201 aaatttaagt gccaccgttc acactgggat ggatcggatt cgtcaaagtg tccatgacct
    10261 ccatgagaca tcgatctctt ttcaacactc cttagagttt ctgatcgctg attttccttc
    10321 ttctgttacg attgaaggaa acttgtttga aggattgaat gaaacgcagc aaaactgctt
    10381 cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa catagtcatg ccaaaaccgt
    10441 tgttttacat tttcgaacgt tgccggcctt ttatcgcttg caaatcacga atgatggcac
    10501 cagcccacaa aaaaattctg ctcccggtat tggtttgaaa acgatgcgtc aacgtgttca
    10561 agaacaaaag ggacaagtcc atattttcca agaaaaccag caatttcagt taacgatcat
    10621 tttaccaagg gagggacacc aatgataaca gtagtcatta ttgatgatga tccatttgtg
    10681 acgaaatcgt tacaaaccat tttagaaagc acgaaagaga tccgtgtgtt aggcattggt
    10741 cattgtgcca aggatgcgct agtcttatac gaaacccatc gtcccgatgt tttgctgacg
    10801 gacatccgca tgcctaaaca aacgggcatc gatgctgcca aagaaatttt ggctaaattt
    10861 cccaaagcca tccttttgct tttgacgaca ttcaaagatg aagaatacat ccgcgaagca
    10921 ttttccatcg gtgtcaaagg gtatttaatc aaacagaact tgcaagcgat tattccttct
    10981 gtcaaatccg cttataatgg acaggccgta tttggtaatg aaatcatcga gactttcact
    11041 caaatgatga agaataaacc gacgattgat agtcatgctt atgcttcttt tagtgaacgt
    11101 gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca ataaagaaat tgcagatgcc
    11161 ttgtacttga gtgacggcac tgttcgcaat tacatcagcc aattactaga aaagctagat
    11221 ttacgggatc gcacgcaatt ggctatctat ttttatcagc atttgcaata agtcttatct
    11281 aacacaaaaa aacctgaatg atgctccttc gcatcattca ggttttt
//
LOCUS       DAPBBG010000075        11230 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPBBG010000075 DAPBBG010000000
VERSION     DAPBBG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.078"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            225..653
                     /locus_tag="RD477_002486"
     CDS             225..653
                     /locus_tag="RD477_002486"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7634803.1"
                     /translation="MSKVDKNRNKKIQDASRNNNWEEVSRLLDQPLENSLRKDRQYKT
                     VSMNNQVSYNGSLKEYGDNIADTNPDPLEQLIINEEEQHLDHAISRLSKQEQLLILGY
                     YFDSKSYARLAKELDISDKTLKKRLQSTLEKMKTILHENF"
     gene            779..1093
                     /locus_tag="RD477_002487"
     CDS             779..1093
                     /locus_tag="RD477_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634804.1"
                     /translation="MNTRHKKGGYSAQIANDYIDHKQPIHSLSIELEKQFRFEDGKPT
                     KEVTGYKAWFTQEGLPPFVVKFSEAIKLPNYLSLIQFDNLEACEVNYNVYFKASGITE
                     VK"
     gene            1093..2169
                     /locus_tag="RD477_002488"
     CDS             1093..2169
                     /locus_tag="RD477_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002272712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDT7634805.1"
                     /translation="MTKKELEQYLLQSLNMALGTLMQGETSYTNSFDCQVTEEGFFFL
                     PRLPAGYIIDDDLYQKIFLIANASLYPRFTLLKQNSAYFVPIDTNDIHIQRALFFPWK
                     KGISERLVIPDLGHFVSSIGDANIPIMKNYSVDYNKVTSILIAGNSGSGKSYALTYFL
                     SILQKFSDLILVDPKFDTPSRWARENSVPVIRPQQNRSKSDFVSEINENLSNCLTIIQ
                     KRQEILYENPSHEFTHLTIVIDEVLALSEGVNKTIKESFFSLLSQISLLGRATKVHLL
                     LVSQRFDHTAIPVSVREQANVLIQIGNINQKTTQFLYPDLDPEGIVIPIGKGTGLIQV
                     IDNEHPYQVLPLLCPTYYTKRGIL"
     gene            2133..2771
                     /locus_tag="RD477_002489"
     CDS             2133..2771
                     /locus_tag="RD477_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634806.1"
                     /translation="MSHLLHETRYLVMKKSYFMRSLNVTIFLLMIALFTLFLVFLSNF
                     LVVSLSESDTKLIQQTSNVIKTISVSLSKTGLLSATVAVLIIAIEMFNRFKIDKIMNY
                     FKSIYSTMLLRHFLTQTERTEKVMTIETQTITTYNPVNNTFNRAVSKCVVDIRQDFVT
                     IFIKTPSKQQAQKLLKEMESQIKEEIANRNPDYYFSAPIRTKNALWFEGKRR"
     gene            2959..3561
                     /locus_tag="RD477_002490"
     CDS             2959..3561
                     /locus_tag="RD477_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDT7634807.1"
                     /translation="MAKERRSNKWAFLMYEESVPDNYMEILEGLHVPFVLSPWHDKDI
                     HSETGEIKKSHKHGAFYFDSLKSYSQVSELVSDKLNGPAHVEVVMSPKGMFDYFTHAE
                     NPDKTPYYIENIESGAGFELDKFLLEQNTDNFMNDVIDIIEENNFMEFEDIVRYARDN
                     NYLLLGLIIERTYFFAKYLDSRRYSQSKNKAKPTEVKEDG"
     gene            3554..3736
                     /locus_tag="RD477_002491"
     CDS             3554..3736
                     /locus_tag="RD477_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634808.1"
                     /translation="MDKEPLLLNRQQASEYLGIDPKSFDKYIRCHPDFKCFMVGKQER
                     YLKSELLKFLEAHCVN"
     gene            3826..4869
                     /locus_tag="RD477_002492"
     CDS             3826..4869
                     /locus_tag="RD477_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7634809.1"
                     /translation="MATITKRGNSYRATVSLYKKGKYKRETKTFKNKKDAELWTLEME
                     LEKGRGKNIAERSTLFPDFYRNWVETVKKNDVREATYTNYMRTLTVVDSLFQGVQLKH
                     LDDIVMQKKLDHYAETHSKKTTHELVLKIRGSLKYAYARGLITNDFGHLLKARGQEQP
                     KRNIALSITNFKKLRQYCLSHQDEEFNILVLLALETGARRGELLGLTKDDLFEYGINI
                     RRSISPTDNDTQLKTKHSKRDISINKDVYIALQNLAETKLTYLFDWNGFHQSSQLQRL
                     LNKLDIPKTTFHGLRDTHASFLFSKEISIDYISRRLGHNSILTTQNYYLELMPEKKHQ
                     QDADALELLNDLS"
     gene            complement(4997..5614)
                     /locus_tag="RD477_002493"
     CDS             complement(4997..5614)
                     /locus_tag="RD477_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7634810.1"
                     /translation="MAKIRYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTRINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(6297..7292)
                     /locus_tag="RD477_002494"
     CDS             complement(6297..7292)
                     /locus_tag="RD477_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7634811.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(7387..8259)
                     /locus_tag="RD477_002495"
     CDS             complement(7387..8259)
                     /locus_tag="RD477_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7634812.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(8272..9699)
                     /locus_tag="RD477_002496"
     CDS             complement(8272..9699)
                     /locus_tag="RD477_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDT7634813.1"
                     /translation="MTKKAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(9753..11027)
                     /locus_tag="RD477_002497"
     CDS             complement(9753..11027)
                     /locus_tag="RD477_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634814.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         3726 a         2140 c         1701 g         3663 t
ORIGIN      
        1 taataatata agtatacaag ataacttctt gtgcacaaat tattaaagtc cttatagtgc
       61 gcactaaacg gacggattga aacaaatatg atgactagac aacaacagtc ttgtctttgc
      121 taaatattgt ttatccgttt atttggtgtg cttttttcac caaaggttca attactagct
      181 cgtgtatgtt tctttctgtc cgctaaggcg gaaaggacat aatcatgtcg aaagtagata
      241 aaaatagaaa taaaaagatc caagatgcta gtcgtaacaa taactgggaa gaagtatctc
      301 gattacttga tcaaccgtta gaaaattccc tacggaaaga tcgtcagtat aaaacagtca
      361 gcatgaataa tcaggtatcc tataatggta gcttgaagga atacggtgat aatattgctg
      421 atactaatcc tgatcccttg gaacaattga ttattaacga agaagaacag caccttgacc
      481 atgcaatttc acgcttatca aaacaagaac aactgcttat cctaggatat tattttgata
      541 gcaaatcata tgcacgtctt gctaaagaat tggatattag tgacaagact ttgaagaaac
      601 gccttcaatc aacgttagaa aagatgaaaa ctatccttca tgaaaatttt taatatattc
      661 gtagctaggg actccgagtc cctagctttt ttctccctat acatgaggag gtaaaatact
      721 gaaacaaaaa gttcaaataa tttgatgagc taataattat agaaagaggt acttatccat
      781 gaatacacgg cataaaaaag gcggctattc agcccaaatt gcaaacgact atattgacca
      841 taagcaacct attcattctt taagtataga gctagaaaaa caatttcgtt ttgaagacgg
      901 aaagcctaca aaagaagtca caggttacaa ggcttggttt acacaagaag gtcttccacc
      961 gtttgttgta aaattttctg aggcaatcaa acttcctaac tacttatcac ttatccaatt
     1021 cgataatttg gaagcttgcg aagttaacta caatgtctat ttcaaagcaa gtggaattac
     1081 agaggtcaaa taatgactaa aaaggaatta gaacaatact tgttacaaag cttaaatatg
     1141 gcattaggta cactcatgca aggtgagact agctacacta atagtttcga ctgccaagtg
     1201 acggaagaag gttttttctt tcttccccgc ttgccagcag gctatatcat tgatgatgat
     1261 ttgtatcaaa aaatattttt gatcgcgaat gcttcattat acccacgatt tactttgcta
     1321 aaacaaaatt cagcctactt tgttccaata gatactaacg atattcatat ccaacgagcg
     1381 ctgttctttc catggaaaaa aggaatttcc gaacgattag tcattcccga tttaggacac
     1441 ttcgtttctt ctattggtga tgcaaatatt cccattatga aaaattattc cgttgattac
     1501 aataaggtca cttcgattct gattgcagga aattcaggtt ctggaaaatc atacgcccta
     1561 acttattttt taagcatatt acaaaaattt tctgacctta ttcttgtaga cccaaaattc
     1621 gatactccca gtcgttgggc tcgagaaaac agcgttcctg taattcgccc acaacaaaat
     1681 cgctcaaaat ccgattttgt gtcagaaatc aatgaaaatt taagcaactg tttgactatt
     1741 attcaaaaac gtcaagaaat tctttatgaa aatccaagtc atgaatttac acatttaacc
     1801 attgtcattg atgaagtatt agctctatct gagggtgtca acaaaactat caaagaatca
     1861 tttttctctt tgctatctca aatctcctta ttaggacgtg ccactaaagt tcatctttta
     1921 ctagtaagcc agcgatttga ccatacagcg attccagtct ctgtacgtga acaagcaaat
     1981 gtgctcattc aaattggaaa tatcaatcaa aagacgacac agtttctcta tcctgattta
     2041 gaccctgaag gaatcgtgat tccaattgga aaaggaactg gcttgattca ggtcattgat
     2101 aacgaacatc cttatcaagt gttgcctttg ctatgtccca cttattacac gaaacgaggt
     2161 atcttgtaat gaaaaaatct tattttatgc gtagtttaaa tgtaactatc tttttactca
     2221 tgattgccct gtttacttta tttttagttt ttttgtctaa ttttctcgta gtatctcttt
     2281 ctgaaagcga cactaaactc attcaacaaa cctcaaatgt tataaagaca atcagtgtgt
     2341 ctctatcaaa aacaggactg ctatctgcca ctgtggcggt tttaataatt gctattgaaa
     2401 tgttcaatcg gtttaaaatc gataaaataa tgaattattt caagtcaatt tactccacca
     2461 tgttattgcg tcatttccta actcaaacag agcgcacaga aaaagtaatg actatagaaa
     2521 ctcaaactat cacgacatat aacccagtta acaacacgtt taatcgtgct gtgagcaaat
     2581 gtgttgttga tattcgtcaa gactttgtaa cgatatttat taagacacca agtaagcaac
     2641 aagctcaaaa actactaaaa gaaatggagt cacaaataaa agaagagata gcaaatcgaa
     2701 atccagatta ttatttctca gctcctatta ggacaaaaaa tgctctttgg ttcgagggaa
     2761 aaagaagata aaaattggcg ccgtggctca cgaagtgaaa gccacaggtg ccttttacta
     2821 tcattaaagg gattggggta ctacgacccc caatcccccc aataatcaat aatcaaaaaa
     2881 ataacgttct tccgagttta tacccaactt tctccttatt catgtagaca ccaaattcta
     2941 tttgaaagga agaatactat ggcaaaagaa cgacgctcaa acaaatgggc tttcctgatg
     3001 tatgaagaaa gcgtccctga taattatatg gagattctcg agggattaca cgtaccattt
     3061 gtacttagtc catggcatga caaagatatt cattctgaaa caggcgaaat taaaaaatct
     3121 cacaagcatg gcgcctttta ctttgattca ttaaaaagct actcacaagt atctgaactt
     3181 gtaagtgata aactgaatgg tcctgctcac gttgaagttg tgatgtcgcc caaaggcatg
     3241 tttgattatt tcacacacgc agaaaatcca gataaaacac cttattatat tgaaaatatt
     3301 gaaagcggtg ctggattcga actagataaa tttctactag aacaaaacac cgacaatttc
     3361 atgaatgacg ttattgacat tatcgaagaa aataatttta tggagttcga agacatcgta
     3421 cgttatgccc gagataacaa ttaccttctt cttggtctaa ttattgaacg aacctatttt
     3481 ttcgcaaaat acttggactc tcggcgctat agtcaaagca agaacaaagc taaaccaacg
     3541 gaggtaaaag aagatggata aagaacccct tttattaaat agacaacaag cctccgagta
     3601 tcttggcatt gatccaaaat catttgacaa atatatccgc tgtcatcctg atttcaaatg
     3661 tttcatggtt ggtaagcaag aacgatatct aaaatcggag cttctcaaat ttcttgaagc
     3721 tcactgtgta aattaacttg gtggttgata aaagaagatt cgtcatttat tctataagtg
     3781 actagtctat tttatcaatc acttttaata aggaaggatg ataaaatggc aacaatcact
     3841 aaacgaggca attcataccg agcaacggtt tctctttaca agaaaggtaa atacaaaaga
     3901 gaaaccaaaa cattcaaaaa caaaaaagac gcagaattat ggacattgga aatggaactc
     3961 gaaaaaggac gtggtaaaaa tatcgctgag cgctcaacac tttttccaga tttttaccgc
     4021 aactgggtag aaactgttaa aaagaatgac gttcgtgaag caacctacac taattatatg
     4081 cggacgctca cagttgttga tagtttgttt caaggagttc aactcaagca tcttgatgat
     4141 attgtcatgc aaaagaagct tgaccactat gctgaaacgc attctaaaaa aacaactcat
     4201 gaacttgtgt taaaaatccg tggctcgtta aagtacgcat atgcacgagg actgattaca
     4261 aacgattttg gtcatttgtt aaaagcacga ggtcaagagc aaccaaaacg taatattgct
     4321 ttatctatca ccaattttaa aaagcttagg caatattgcc taagccatca agatgaagaa
     4381 ttcaatattt tagtattact cgctttggaa acaggtgctc ggcggggtga acttttaggt
     4441 ctgacaaaag atgacctctt tgaatatggc atcaatatta ggcgctcgat tagtcccact
     4501 gataatgata cacaactcaa aaccaagcac tctaagcgtg atatctcaat caacaaagac
     4561 gtgtatatag ctttacaaaa tcttgctgaa acaaaattaa cttatttgtt tgattggaac
     4621 ggattccatc aatcttcaca actccaacgt ctgttaaata agttggatat tccaaaaaca
     4681 acgttccatg gcttgcgaga tacacatgcg tcatttttgt tctctaaaga aattagtatt
     4741 gactatattt ctcgtcgctt aggtcataat tcgatactca cgacccagaa ctattatctt
     4801 gaattgatgc cagaaaaaaa gcatcagcaa gatgccgatg ctttagaact gttaaacgat
     4861 ttatcataat taacaccaac tgacaccaaa aagtgccaga aaacgttgat acaataagga
     4921 attgattaac gttttgagaa ttgtgaagct ttacgagctt tcttaagacc tggtttcttg
     4981 caaaattata ttagcctcaa ctgaaagatt tatttttttg ctccctaact tcattagtaa
     5041 taccatgttt cattcgatat cttctaaacg ttctacgatt aatccttgtt agctcttcaa
     5101 cttctttatc tgtagatcca gtcaaaaaca aattaaatgc taattgaagt cgcggatcat
     5161 tctcacgata ttttagcgta cctcccttgt atttcccttt tgctttagcg agtgcaattc
     5221 cttgttgttg cgtttcgcga atataccgac gttcttcttc ggcctggtac ttgtatagtt
     5281 cgataattaa gttattaagt aatcgacgta aattatcatc agtgatacct gttaacgctg
     5341 gcaaattcaa aacttccagt gtagctttct tcagctgaat taaattcatc gtttctgtta
     5401 gctctttatt atttcgtcca agtcgtttta attctgtcac aacgacaata tcaccttcac
     5461 gtatgaattt catcatttct tgaaatcctt ctcgattggt atctttccca cttgctttat
     5521 cactaaaaat tttatcacac tctgataata attctaattg tcggtctaaa ttttgatcta
     5581 agctgcttac tcgagcatac ctaattttcg ccataataaa actcctttaa aattctgaaa
     5641 tccggatcat ttttaaattc tgttgcccaa ctctgttgtc atttttctag ataaatggca
     5701 taaattttca gcgctaagtg acaaaagcaa ctaatttttc atgatagatt tttaaatatc
     5761 tcaatctatc tccaaaatta gttgcttttg tagctatagt tgactatgaa tatgacaaat
     5821 aattagaaaa aactgtgcca attaaatata aaaatgacac tcatttacat tcaatagaac
     5881 accaagagcc caacagggta tactctggtg tccttttaaa aaagtaactt tctgacctaa
     5941 ttaattgagc tactaaaaaa gctatgaaat gaggaataaa aaagggatca aaaactttta
     6001 agtttctgac ccttttgagt tctaattatt tcttccagaa cgaagaatgt tatctgcttt
     6061 atctgcaatt gattctgtta gaaggactga aaaatttatg ccagcccttt tgcctagctt
     6121 atccgcccat tgaggaattg ttaaggtttt tctgacaagt tctatgtctt tcaaatattt
     6181 ggcaacatct acacagatta aaagcatgat tttgtatcat agtctattat aagagattta
     6241 acaatttttt atatcacgaa ttaataatga aattatcatt gatagtcttt ataaatttaa
     6301 ttgttctttt tctttactga tgtcttcccc atccataatg tgacaggtaa aatatattcc
     6361 ttttttgttt cttctttatt tattcgtttc atcaaaatag caacagctgt gtctgctata
     6421 tcattaattg gttgtattac tgttgtcaga tctggtaata tactccgagt cattaaagta
     6481 ccatcatatc cgactactaa taaatcagct ggtacattta accccttttc ttttgctact
     6541 tgtaatatta gggccgcatc gatgtcattt gaagcaaaaa taccatctat tttaggataa
     6601 tcctcaaaca tttggaaaaa aatctgtttt ttctttacat aagaaatatt gaaatctaat
     6661 gtcacagttc gtggaattaa ctgatatgct ttcatagtat cttcataggc tagtctccga
     6721 cgattagccg gagtctgtaa gtcaattgga ccgtttgtat gaataatatt ttttgcacct
     6781 tgtgcaatta ataacttggt tgcaagtttt ccaccattgt agttatcgga ttcaacaact
     6841 ggaatatctt cattcatcac tcgatcaatt gcaacaatcg gtaaattctg atatttatat
     6901 tcctgaattc cttggttatg tgtaccaaca attaaaccat ctacttgatc agacaataac
     6961 tgatttaaat aatttgtttc cttttcttta ttattcatcg aattgcctat taatacctta
     7021 taaccaattt catataactt tttttccaat gcctcaacga gttcactaaa aaaaggattg
     7081 gctactgtag gaaatagtaa cccgacaatg ttagttttct tttgaaataa ttgacgcgct
     7141 acaacgttcg gttgataatt gagttcttct atagccttat aaacattatc aatcgttttt
     7201 tgacttaaat agccacgatt gtttaataca cgtgaaacag ttgtttttga tacatttgct
     7261 cttttggcaa catcttctaa ttttgggcgc attctcttgt ctctcctaaa taatgcaata
     7321 attaactatc gaaaaaaaga actctataga actactatac ctgataatcg atattttttc
     7381 tatgttttat tcttgtaatt gattcaagcc caaagcaaag tttcctagtg ttgccgatcc
     7441 attattttta attaaaggac gcgtaatata tttacttaac ggtggtattt ccacgtaatc
     7501 atccaataat ttattaacat taacacgaat tttttctaac aatttttcat ttactacgct
     7561 tccaccaaat actattttat ccggtcgtat aatcaatgtt gtttgaatca cagcttgtgc
     7621 tagataatag gccattacat cccaaatagg atccgttaaa gaaacgtctt cacccttttt
     7681 tccaaaacgt gcttcaaacg ttggtcccgc aactaagcct tctaaacaat caccatgata
     7741 aggacaaatt cctttaaaat caatatcatc aggatggcgt tttacaaacg tatggcccat
     7801 ttctgaatga ccaaacgatc ctaatagttt tccgtcaaca acagctccac caccaacgcc
     7861 agtaccaacg gtgtaataaa ctaatgaatt tattttttca ttcgataaag tggacatgat
     7921 gtactctcca taagctgatc cgttcacatc cgttgtccaa gacattggaa cattcaactc
     7981 atcttgtaaa taacctaaca cattaacatt tcgccatccc acctttggtg tagacgtaat
     8041 atatcctgat tttggtgaat tttttcgaat ttctattgga ccaaatgatg caatacctaa
     8101 tgcatttata tcaaactgtt taaaatagtc aacagttttt ttcaatgttt ccctgggggt
     8161 ggtcgttgga caaattattt ggtcttgtac tcggtaatcc tcgtctccca cggcacatac
     8221 aaatttagtc ccacctgctt ctatgctacc aattaacatc ataaaacctc cttactcgta
     8281 agaaattgca tcttttttta gatgataaat tgtactcgag acaaaagctt cttcactaac
     8341 actcaaatgc atacttggtt gattttctgt ataataacgc tctgtaaaaa caagctcgcc
     8401 atcattaata aaaatctctg ccgaactttt atcgataaac actcgaagtg ataatctatc
     8461 acacatctga atggttccat accttggatc atttttgccg ttacgagtta aggtacattc
     8521 atttttatcc ttagaataaa aaatattgat taactcttca tcttttgttt ttaatgaaaa
     8581 tttgatttct tgattttttg atttagtcaa tagtacatca attagagctt ctttgaatga
     8641 agcatcttga gcgattatta tttctcctgc caaattaact gtcgtttcaa cgccgctgcc
     8701 aactcgaagt tgttctaatt ctctcacagg tttcatatac aaacgatctc caattaactc
     8761 taattctcga ggaagcgtta atgctcctgc ccatccgtct actttttcag gcatagggct
     8821 ttcccacata tccatccagg ccataacaat acgtctacca tctggagata aagttgtttg
     8881 tgttgcatag aaatcgtgtc ccttatctaa ttcataaaaa tttcctctaa tgaattttgc
     8941 tgtctcataa tcataatcac cgataaagta gcctgtttca tatagattta aataatcttt
     9001 tccatttgct tcaattccct gaggggataa taaaagaata gattttccat ttaattcaaa
     9061 aaagtctggg cattcccaca taaatccttc tgtaagtact tccttcgctt tagaaattgg
     9121 tccaatgtaa tcccaattta ataaatctaa cgactgataa acaatagcac gacctaatcc
     9181 atctttttct tgactaccca aaaccatgta atatttttca tcgtgtttcc atacttttgg
     9241 atctctgaaa tgatgtgtgt tatcttctgg tgcttttgct attacaggat ttttttcata
     9301 tttagtaaag tgaatgccat ccgtactata cgccatgttt tgattttgcc aaaaatgatc
     9361 tggatcattg tctccataat agtgatgtcc tgtatagaac agatacaaca caccattttt
     9421 ttcaatagcg cttcctgaaa aacaaccacc ttcatcttct tgatctccag gagtcaaagc
     9481 aagtggtaaa gattcccaat gaactaaatc cttacttcta gcatgtgccc agtgcattgg
     9541 tccccattca gcactatatg gatgatgttg ataaaacaca tgataatacc catcaaaata
     9601 gcaaaatcca tttggatcat taatccatcc tgatggtgca gacacatgat atcccaaacg
     9661 ataacgtgta tttgttaatt ctatagcttt ttttgtcata atatattcgc tcactttcta
     9721 gataaaattg ttttttattc tgttggtttc cattatcctc gaataaatgg atccccttga
     9781 acatctctat catcttgttt aagaataaag aatgcatata tgcctgctag taatacaata
     9841 agagaaataa ttttaaatgt tggttgatag ccaacgttgt ctcttaatat tcccaatggt
     9901 gttgataaaa tcacttgccc aatttgagca gctatctgaa atcctatcat atagagggtt
     9961 gctgatagct ttgtatcaaa atgaagtgta aaatagcgaa acattggtaa tgtaaataat
    10021 ggtacttcta aagcatgcaa catttttatg catgaaacag ataatggtgt cgaggcaaac
    10081 ccgcacaatc caattcgtac acacataatg gttacaccta ataacaaagt atttcgaacc
    10141 cctaattttc tcataatgat tggaacaatt cccatcatta acgcttcaca aaatacttga
    10201 atagcattca atgtcccata tattttttca ccattagctg atgttgaaaa caaaccagta
    10261 taaaatcccg gaaacatttg ttgatcgaat accgtataaa atgtccatgt aaaaacaata
    10321 aagatgatta tcgtccataa ttgaggcagt ttcattagat caaacatttc ttttaaagaa
    10381 ggaatactat ttgattcagc ttgttcttga ttaaaatttt gatttgcttc ccgttcaact
    10441 ttagggttcc aaaataataa atttaacaat agtaaagaac caaagaaaga tcccgcccaa
    10501 aagtttaaat gaggattttt tacaaataaa aatcctgcca aaagcgctac caaggcataa
    10561 ccaaatgatc cccacgccct tgcttgtcca tattcaaagc caaataaacg actaaacctt
    10621 tctgacacag cttcaaaaac tcctacagaa gctaaatatc cagctgacaa aaatagcgct
    10681 cccataataa tgcctaaatt aaaattgtta tctagcaatg gcccatatat ccatataaaa
    10741 aatggtccaa caagtgtcgc taatacagaa gcaaaaatta ataaatttcg tttaatatac
    10801 aatttatctt gtaatgttcc ataaataaac atcaaaatta aggtaactaa cgaatttgcc
    10861 gagaatactg ttcctacagc actgccagat aaccctaaac catttgattc agatgttagc
    10921 caaagttgaa aaaatgacca ccaaacaccc caagaagcaa aaaatagtaa taatgtagct
    10981 gaactttgta aataagattg atttttaaat acgttaactt ttgacatatt tttatcctcc
    11041 aacgatatat ttgtatcccc tttcatgata accggttaac atttttagaa aaggacttgt
    11101 actcctaaaa ttataaaaga aatccgctta catgtcaacc ggttattcta tttggataat
    11161 gccatataat gtcacaggat atgactttta ttggtgtgtc gcttttcttt tttaatggca
    11221 ctatttttta
//
LOCUS       DAPBBG010000076        11228 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPBBG010000076 DAPBBG010000000
VERSION     DAPBBG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.079"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="RD477_002498"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="RD477_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDT7634815.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="RD477_002499"
     CDS             complement(2944..3378)
                     /locus_tag="RD477_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634816.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="RD477_002500"
     CDS             complement(3395..4600)
                     /locus_tag="RD477_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDT7634817.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="RD477_002501"
     CDS             5294..5680
                     /locus_tag="RD477_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7634818.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="RD477_002502"
     CDS             5774..5965
                     /locus_tag="RD477_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634819.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="RD477_002503"
     CDS             6479..6811
                     /locus_tag="RD477_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7634820.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="RD477_002504"
     CDS             7107..7262
                     /locus_tag="RD477_002504"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDT7634821.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8878
                     /locus_tag="RD477_002505"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="RD477_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="RD477_002506"
     CDS             complement(9119..10735)
                     /locus_tag="RD477_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDT7634822.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="RD477_002507"
     CDS             10853..11071
                     /locus_tag="RD477_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634823.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
BASE COUNT         3677 a         2084 c         1689 g         3778 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat aaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac aaaaaccatt caaagtagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tacctttcat gaggatgcct ttcgcaaaga
     7621 gcgcatcaat gacgaaacat gttttgcttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacaga
//
LOCUS       DAPBBG010000077        11170 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPBBG010000077 DAPBBG010000000
VERSION     DAPBBG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.080"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(58..231)
                     /locus_tag="RD477_002508"
     CDS             complement(58..231)
                     /locus_tag="RD477_002508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634824.1"
                     /translation="MKNSKEEGKNCIFCKKKINSAKKISICSSCRKRSKDSLSALGIV
                     SLSLVGISKKMKK"
     gene            complement(221..1378)
                     /locus_tag="RD477_002509"
     CDS             complement(221..1378)
                     /locus_tag="RD477_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634825.1"
                     /translation="MDEQKETFEISKTVKNNIENFELFNSYVDKYTQDIFEMSSPYEF
                     LLPQYMEKGGTGELSNIFSLDGNSLLNLAGTSINLKELLFSSKNGFVYQPDFDSLSND
                     TKEKIKSGIFKIGESKKIEGNLRAVIVDSTNSNRRVEDLTLKEVVVDKNIARTLTDLA
                     VQAQLKQIYEIVVDIQETQEYQLHWDRNNSILAPFFTARTKILEFKKSGDLNRKIQLL
                     EAASDSMEQAVSAIKADLISNKDHIKKILKKPMYRYSTFERHANFILSDINLLLKIVG
                     IQTYIDLTLDNEEVANDRLEAVKYIFELYSSRNIGATGNIILNTLERLPFINNVIPDV
                     KKVELPSLLEIIHDNYRYTSENKDSWLNINDEMKEGNQFSLLGEGGSEYEE"
     gene            complement(1475..2188)
                     /locus_tag="RD477_002510"
     CDS             complement(1475..2188)
                     /locus_tag="RD477_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634826.1"
                     /translation="MRKTVGLFRPLRFDHLDLTAKLLVKYSIPDTFEILDDVLAKEFK
                     GKEGRRKIINNLTRVWSGGKKEPSTFQKEVLAKYNVSDKNDKIIYQYLMTSLAFPFFS
                     ETTTFVGKYLKMSDTFKSSVILSEMRNSYGSAESVYKGVNAVLNGLKSWEILSEGNSK
                     NHYVFKDNLLEISNEFQLNLLVLVVIQNHVGDSLTIEQINNHASMFPFRYLIRKEDIV
                     SSQFEVIEDRIDTYISILS"
     gene            complement(2201..3664)
                     /gene="pglZ"
                     /locus_tag="RD477_002511"
     CDS             complement(2201..3664)
                     /gene="pglZ"
                     /locus_tag="RD477_002511"
                     /inference="COORDINATES: protein motif:HMM:NF033449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system phosphatase PglZ"
                     /protein_id="HDT7634827.1"
                     /translation="MGWEDKIRNMFPRAGEVYLVKDRDNILDDSSVIDMFSRNNIDYI
                     DFLDPIVFRYAYETKYKYERTNTLIIRTDNYSEIPYDVYSNSFQVQIKLKDVITFLDY
                     SAMKEYSIGIPELVHIDSFQNKTLNYEETKRLLSKDQSREDSEEYVLPVANDITTFGL
                     VVMIIGQWRSGDNTLNDKQISSINDVNSKFQNWLIQNFDQTVSLPSYPKPKMVHQIPD
                     FMNHSDYNQKKALIVMDGMSFTQWNIINDYLINNQFKTEVSPTLAWIPSITSVSRQSI
                     FSGKPPLNFSDTINTTNYEEKQWEDFWVDKGFDKSDIFYQRALGSDNYLEMDLQHILR
                     NKKIVGCVIDSIDRFMHAAQQGLWSVHSELNYWLNKGFLLSFLKDLIENDFEVYITSD
                     HGNIESQGVGRINQGVLANSKGERVRVYDDKNIRNRTREDYFEVSENWDSQFLPQNYY
                     PLIAKENYAFITKNDKIVGHGGTSIEEVFVPFVKVTR"
     gene            complement(3676..6486)
                     /locus_tag="RD477_002512"
     CDS             complement(3676..6486)
                     /locus_tag="RD477_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDT7634828.1"
                     /translation="MDQGSLFEEENRPVECLGMTFESDNERREYFRNELRKKLPYLKS
                     IEGFPIGEDEDIIELSDPPYYTACPNPWMNEMIDNWENEKKYENKVVQHAYVSDVSEG
                     KNDAIYTSHTYHTKVPYKAIMRYILHYTNPGDIVLDAYAGTGMAGVAAYYCGKEEEVA
                     NLGILSRKDDIQIDEESFKVGVRHAILNDLSPAASYISNMFNSPRDSSKIYLEIKNTM
                     NDIKSDFFWMYKTITTEDDELKGKMTEELKRINSLDEMKEYMVRYDKSLGDIQYVVWS
                     DVFVCNNCGNELTYFDNGIDWKNHTTLKQFSCPKCDNILNKRSLEHLFEQSYDKFLDK
                     NVSLAKQVPIKINYVYKNKRYNKEIDSFDLNLIEKISRLIEYNNFPIDELPNGYNTKQ
                     PKESHGITHVHQFYTIRNLFIASEFWRRLDWNNRWILTSFISRNATKLNRFVINKYNP
                     NGRINGPLSATLYVPSLSVEQSIFILIEDKLRDIDWNTYGNIVEVSSASDLRINDESI
                     DYIFIDPPFGANIMYSEVNYIWESWIKVKTNNKEEAIQNPTQDKDINSYKNLMIKGLK
                     ECYRVLKSGKWITIEFSNTQAAVWNVIQSVVQEAGFIISSVGVLDKKHGGIRAMTNPT
                     SVKQDLVISAYKPNDAFKNELINNQNSILSIYYFLDYHLEKLPTFIGRNKEAELIVER
                     TPKVLFDRVVAYHIQNGLSVPVSSAEFQEKVAQKYVMRDGMVFLESQVAEYDKKRILA
                     KEFSQQSLFVSDESSAIEWLRQQLMKKPQSRQDLHPQFMKEIQHIAKHEELPELDKLL
                     AENFLLYDSEDVVPDQIAAYLRKNYHDMRGLENQDDKLKSKAKNRWYVPDPNKQADLE
                     KLREKSLLREFNHYVEEVSGTKKKLKLFRSEAIRAGFKQAWSEKDYQKIVDVAEYLPE
                     KIIQEDDKLLMFHTHALMRLDI"
     gene            complement(6494..10216)
                     /locus_tag="RD477_002513"
     CDS             complement(6494..10216)
                     /locus_tag="RD477_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7634829.1"
                     /translation="MKYKDLVNFEPIDSVIQLTDANNKDRALHLLDTFVISDRMADTI
                     NNTIINQLQFSKPVDNKGLMIVGNYGSGKSHLMSVISTIAEYKDSSQHLQNESVAEKA
                     QEIEGKFKVIRFEIGSTEMSLREIITQQLELGLLDLGITFEFPNADQIVSNKNSLLEM
                     MSIFNEKYPDKGLLLVVDELLDYLRGRKEQELTLDLGFLREVGEIAHNTRFRFIAGIQ
                     EMLFDNPRFSFVADSLRRVKERFEQVRIVKEDISYVISERLLKKDEKQKALVREHLTS
                     FTKLYSRLAEDMDKFVDLYPIHPSYLTAFEKVHNIEKRVALKTITMEIQKIIDHDVPV
                     DSTGVVAYDNYWTFIEEDPSNKTIPSVREVLEKTAILKDKVNSSFPSDRKGQMYKDVS
                     LQVINGLALNRLTMDDIYSPVGLTASTLKDDLFLVIPGQLDFLLEDDDPSAFLETSVS
                     VAIKMIMETVSYQYISVNQENGQYYLDLKKDIDIDSLISERTEIIENATLDSYYYKIL
                     QNAVALDDNTYVSGYRIWQHELPWEQRRVMRRGYLFFGSPNERSTAQPERDFYVYMLR
                     PFEKVQYKDELKSDEVFFELSLEDKNFKMNLLNYAAANELYNETTSAQKRLYKDKIDS
                     YFKKLNKWLTERFVDAFKITYKGKTGYLNDFGMFLPQEDNVKSLVNFSAQEFLAEHFS
                     EKYPEYPSFRNYKPGFISSNNIKVVASDALQRINGRNSIQGESVLEGLVLLSNQKTIT
                     SESVTNSAYARWIIETLEIKGNGKVLNNDELFNINAVRGVEDRRLSKSFDLEPELVVV
                     LLGALLSTGKIEVNIDGTTYTAMNFSEFAVLSMEKLTKFSYIKKPSGLPLIEINEILN
                     LFGESAPNNNEEVLEGSIRRMNSKLNTSIEEALKAKRDLSIGYLVGNLDVLDNNTRSE
                     YADQLESFKLFCEQLLRYNSIPKMKNLNVSISEIEKQKQNKELVESLNILKKNVDEVS
                     QLVNYLDQAKYSYGQNSDWSKTIDETVSKLRSNISDGQSYMSNLSELRQLKEEYITQY
                     YTIHEQSRLTATEDNKKQEILRSPQLNMLRRLQSSISLLPGEQLDNWQENLERTKACY
                     QLKKTDLEMSPTCPHCNFLLSIDKRDSRNIVENSMDKLTDIYDNWLNILIDNLKQDSI
                     QDGLTLLTNDEQRSIELLISSKELPLPLNQRFIDMIKNLFDGFEKVELSKEDIIQMLG
                     NGSPMSIDELEGRIRELIATAVDGKDRDKVRIMYKG"
     gene            complement(10228..10686)
                     /gene="brxF"
                     /locus_tag="RD477_002514"
     CDS             complement(10228..10686)
                     /gene="brxF"
                     /locus_tag="RD477_002514"
                     /inference="COORDINATES: protein motif:HMM:NF033453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system P-loop-containing protein BrxF"
                     /protein_id="HDT7634830.1"
                     /translation="MEDIEWLVKMLEEKVEEKKLDYNQFMVLLWNNNTEIKYLFKEID
                     SSILNINRLISEQLLDIPSKLQAIKTQQIITEVIRNNPQKVIVIDNFEILFSPIVKVK
                     PIELFRELSRYKTIIVIWRYSYENNRLVYAVPEHIEYQEAQINKNEMIIN"
     gene            complement(10854..>11170)
                     /locus_tag="RD477_002515"
     CDS             complement(10854..>11170)
                     /locus_tag="RD477_002515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634831.1"
                     /translation="KIKSELSNSFIFDLDIKKEKEKLSDKYKDFTFTEDKIKSISSSI
                     YSYEKYQLEKLEIEISKDNSIISESAQFRYIFGITLREQVEKLHYDNELLLRSLDNIG
                     IR"
BASE COUNT         3521 a         2033 c         1329 g         4287 t
ORIGIN      
        1 taaaaatcca ttaaattgga taaagttaac accaattaac accataaaat agactagtca
       61 ttttttcatt ttttttgaaa tacctactaa actcaatgat acaattccta aggctgagag
      121 actatctttt gaacgctttc tacagctgct acatatacta attttttttg ctgaattaat
      181 tttcttttta caaaatatac agtttttccc ctcttctttg ctattcttca tactctgacc
      241 ctccctcacc tagtaaactg aattgatttc cctctttcat ttcatcattg atattcagcc
      301 atgaatcttt attttctgaa gtatatcgat aattatcatg aattatttct agtagactgg
      361 gaagttctac ctttttaacg tcaggtatta cattatttat gaacggcaaa cgctccaatg
      421 tattcaaaat aatatttccg gttgcaccaa tattcctact agaatacagc tcaaaaatat
      481 attttacagc ctccagccga tcatttgcga cttcctcatt atcaagagtt aaatcaatat
      541 atgtttgtat gccaactatt tttaacaaaa gattaatatc agataaaata aaattagcat
      601 gcctttcgaa cgtactgtat ctgtacatag gttttttcag tattttttta atgtgatcct
      661 tatttgagat aaggtctgct ttaatggcac ttactgcttg ttccatgcta tcagaagcag
      721 cttctaataa ttgaattttc cgatttaagt caccactctt tttaaactct agtatctttg
      781 tacgagcagt aaaaaatggc gctaaaatac tattgtttct atcccagtga agctgatatt
      841 cttgtgtttc ctgtatatcc actacaattt catatatctg cttaagttgt gcctggacag
      901 ctaaatctgt taaagtacgt gcaatatttt tatctacaac cacttctttt aaagttagat
      961 cttccacacg tcgatttgaa ttagtactat caactattac tgctctcaga tttccctcaa
     1021 ttttctttga ttctccaatt ttaaaaatac cagactttat tttttcttta gtatcattcg
     1081 ataaactatc aaaatctggt tgataaacaa aaccattttt actactgaat agaagctctt
     1141 ttaaattaat agaagttccc gctaagttaa gtaggctatt tccgtctaaa ctaaatatat
     1201 tacttagctc tccggttcct cctttttcca tgtactgtgg gagtaaaaac tcgtaaggac
     1261 tactcatctc gaaaatatct tgagtgtatt tatcaacata tgaattaaat aattcaaagt
     1321 tttcgatatt attctttaca gttttagata tctcaaaagt ttctttctgc tcatccacta
     1381 tttatacaca cccttttagg attctatcac ttcaaactct ggtaattcat caattaattc
     1441 tggagttttc taggcattct ttagattatt actcttatga taatatcgaa atataagtat
     1501 caatcctgtc ttctataact tcaaattgag aagatactat atcttctttt cggattaaat
     1561 atctaaaagg aaacattgat gcatgattgt taatttgttc aatagttaat gaatcaccaa
     1621 cgtgattttg tataactaca agtaccaaga gatttagttg gaactcatta ctaatctcta
     1681 ataagttatc cttaaaaaca taatgattct tactatttcc ttctgataat atctcccaac
     1741 tcttcaaacc atttaaaaca gcattgactc ccttataaac tgactccgca cttccatagg
     1801 aatttctcat ttcagataaa ataacactag atttaaaagt atcagacatt tttaagtatt
     1861 tcccaacaaa tgttgtcgtt tcactaaaga atggaaacgc taaagaagtc atcaaatatt
     1921 gatagataat cttatcattt ttatctgata cattatattt ggctaatact tccttttgaa
     1981 aagtagatgg ttccttttta ccacctgacc atactctagt caaattattt attatctttc
     2041 ggcgaccttc cttaccttta aattctttgg ctaaaacatc atctaatatt tcaaatgtat
     2101 caggtatcga atacttaact aaaagctttg ctgttaagtc tagatgatca aatctcaatg
     2161 gtcgaaataa accgactgtt tttctcattc tatccattcc ttatcttgtt actttaacaa
     2221 atggtacaaa tacttcttca atgcttgtcc caccatgccc aacaatttta tcatttttag
     2281 tgataaatgc ataattttct ttcgcaatta aaggataata attctgcggt aaaaactggc
     2341 tatcccaatt ctcgcttact tcaaaataat cttctctagt tctatttcgt atatttttat
     2401 catcatatac tctgactctt tctccctttg aattcgctaa aacaccttga ttaatccttc
     2461 caacaccttg ggattcgata tttccatgat ctgaagttat atatacttca aaatcattct
     2521 caatcaaatc ttttaaaaat gatagtaaaa agcctttatt caaccaataa tttaattcag
     2581 aatgaaccga ccataaacct tgttgagcag catgcataaa tctgtctata ctatctatca
     2641 cacaaccaac aattttttta tttcttagta tatgttgtaa atccatttcc aaataattat
     2701 cacttcctag agccctttga taaaaaatat cagacttgtc aaacccttta tctacccaga
     2761 aatcctccca ttgtttttct tcataatttg ttgtattaat agtatcgcta aaattcaacg
     2821 gtggttttcc agaaaaaata gattgtcttg atactgaagt tatcgaagga atccatgcta
     2881 aagttggaga tacttctgtt ttaaattgat tatttataag atagtcgttg attatattcc
     2941 actgagtaaa actcattcca tccattacaa ttaatgcctt tttctgatta tagtcactat
     3001 gattcataaa atcaggaatt tgatgaacca tttttggttt aggatacgat ggtaaggaaa
     3061 ctgtttgatc aaaattttga attaaccaat tctgaaattt tgaattaaca tcattaatac
     3121 ttgaaatttg tttgtcattt aaagtattat ctccacttct ccattggcca ataatcataa
     3181 ctactaaccc aaaagttgta atatcattag ctacaggtaa aacatattct tcactgtcct
     3241 cacgtgattg gtctttagat aacaatctct tagtttcctc ataatttaaa gttttatttt
     3301 gaaatgaatc aatgtgaact aactcaggta tcccgatact atactctttc attgctgaat
     3361 aatccaaaaa cgttattaca tcttttaatt taatttgtac ctgaaaagag ttagagtaaa
     3421 catcgtaagg tatttcagaa taattatcag ttcgaataat caatgtatta gttctttcat
     3481 acttatattt agtttcatag gcatatcgaa aaacaattgg atcaagaaaa tcgatatagt
     3541 caatattgtt ccgactaaac atgtctatta cactcgaatc atccaagata ttatcacgat
     3601 ctttcacgag atatacttct cctgctctag gaaacatgtt tcttatctta tcttcccaac
     3661 ccatcataga acctcctaaa tatctaatct cattaatgcg tgagtgtgga acattaataa
     3721 tttatcatct tcttgaataa ttttttctgg taaatattct gctacatcta caattttttg
     3781 ataatctttt tcgctccaag cttgtttaaa tcctgctcga atagcttcag aacgaaagag
     3841 ttttaatttt ttcttggttc cactaacttc ttctacgtaa tgattaaatt ctcttaacaa
     3901 acttttttct cttaattttt ctaaatcggc ttgtttattt ggatcaggaa cataccatct
     3961 atttttagcc ttactcttca gcttatcatc ttgattttct aaaccacgca tgtcatgata
     4021 attttttcgt aaataggcag caatttgatc tggcacaaca tcttcactat catacaacaa
     4081 gaaattttct gccaaaagtt tatctagttc aggcagctcc tcatgttttg caatatgctg
     4141 aatttctttc ataaattgtg gatgtaaatc ttgcctactc tgtggttttt tcattaactg
     4201 ttgacgtaac cattcgattg cactactttc atctgagaca aataatgact gttgcgaaaa
     4261 ttctttcgct aagattctct tcttatcata ctcagctact tgagactcta aaaagaccat
     4321 tccatctctc ataacatatt tttgtgcaac tttttcttga aattcagctg aagagacagg
     4381 tacagacagt ccattttgaa tatgataagc aacaactcta tcaaataata ctttaggtgt
     4441 tcgttctact atcaattcag cttctttatt tcgtccaata aaggttggca atttttctaa
     4501 atgatagtct aaaaaatagt aaattgataa aatagaattc tggttattaa taagttcatt
     4561 tttaaaagca tcatttggtt tatacgccga tatgactaaa tcctgcttta cactagtagg
     4621 atttgtcatc gctcggattc cgccatgctt cttatcaagt acacctacgc tagatataat
     4681 aaatccagct tcttgtacga ctgactgtat cacattccaa acagcggctt gtgtattcga
     4741 aaattcaata gttatccatt taccagactt taaaactcta tagcattctt ttaatccctt
     4801 tatcatcaag tttttatacg agttaatatc tttgtcttga gttgggtttt gaatagcttc
     4861 ttctttattg ttggttttta ctttaatcca tgattcccaa atataattaa cttctgaata
     4921 cattatattt gccccaaaag gaggatctat aaaaatatag tcaattgatt catcattaat
     4981 tctcagatca cttgctgatg aaacctccac aatatttcca taggtattcc aatcaatatc
     5041 tcttaattta tcttctatta aaataaatat tgattgttct accgataaac ttgggacata
     5101 aagtgtagca cttaatggtc cgttaatcct tccattaggg ttatatttat ttatcacaaa
     5161 gcgattcaat tttgtagcat ttctagatat aaacgatgtt agtatccatc gattattcca
     5221 atctaatctc ctccaaaatt cacttgctat aaataaattt cgaattgtat aaaattgatg
     5281 aacatgagta ataccatgcg attcttttgg ttgttttgta ttatatccat taggaagttc
     5341 gtctatagga aaattattat attctattaa tcttgatatt ttttcaatta aattcaaatc
     5401 aaatgaatct atttctttat tataccgctt atttttatat acatagttta tcttaatagg
     5461 gacttgcttt gctaagctta catttttatc caaaaattta tcataacttt gttcaaacaa
     5521 atgttctaaa gaacgtttat ttaaaatatt atcacatttt gggcaactaa attgcttaag
     5581 tgtagtatga tttttccaat caataccatt atcaaaataa gttaactcat taccacaatt
     5641 attgcataca aatacatctg accatacaac atattggatg tcccctaaac ttttatcata
     5701 ccttaccatg tattctttca tttcatctaa agaatttatt cgttttaact cttcagtcat
     5761 ttttcctttt aattcatcat cctcagtagt aatagttttg tacatccaaa aaaaatcact
     5821 ctttatatca ttcattgtgt tttttatttc tagataaatt tttgaggagt ctcttggact
     5881 attaaacatg ttagaaatat atgacgctgc tggagataaa tcatttagta tagcatgtct
     5941 cacaccaact ttaaatgact cttcatctat ttgaatatca tccttcctag ataaaatccc
     6001 tagatttgct acttcttctt cttttccaca ataatatgca gcgacacctg ccatcccagt
     6061 tccagcataa gcatctaaca caatatctcc agggtttgta taatgtagaa tatatctcat
     6121 tattgcttta tacgggactt tcgtatggta ggtatgtgaa gtataaattg catcattttt
     6181 accttctgat acatcggaaa cataggcatg ttgcacaact ttattttcat acttcttctc
     6241 attttcccaa ttatctatca tctcattcat ccacggattc ggacaagctg tataatacgg
     6301 tggatcagat aactcaataa tatcttcatc ttctccaata ggaaatcctt caatactttt
     6361 taagtatggt aatttttttc gcaactcgtt tctgaagtat tcccttctct cattatcgct
     6421 ctcaaatgtc attcctaaac attcaacagg tctgttctct tcttcaaata atgaaccttg
     6481 atccataaaa acatcatccc ttatacatta ttcttacttt atccctatct ttcccatcaa
     6541 cagctgttgc tatcaactca cggattctac cttctagctc atctatactc ataggactac
     6601 cattcccaag catttgtata atgtcttctt tagacagctc cactttttca aatccatcaa
     6661 aaagattctt tatcatatct ataaatcttt ggtttagtgg taatggaagc tccttgctag
     6721 agattaacaa ttctatagat ctttgctcat cattagttag aagagttaat ccatcttgaa
     6781 tagaatcctg ttttaaatta tctattagaa tatttaacca attatcgtaa atgtcagtta
     6841 acttatccat tgaattctca acaatatttc ttgaatcacg tttatctata ctcaataaaa
     6901 aattacaatg tggacaagtc ggactcattt ctaaatccgt tttctttaat tggtaacaag
     6961 cttttgttct ttccagattt tcttgccaat tatctaactg ctctcctggt agtaaagata
     7021 tacttgattg taggcgtcga agcatattta actgtgggga gcgtaatatt tcttgttttt
     7081 tgttatcttc agtcgctgtt aatcttgatt gttcatgaat tgtatagtat tgcgtaatat
     7141 actcttcctt taattgacgt aattcagaaa gattagacat atatgattgt ccatcagaaa
     7201 tatttgaacg tagtttagat acagtttcat caatagtttt agaccaatct gaattttgtc
     7261 cataactata ttttgcttga tcaagataat ttactaactg acttacttca tctacatttt
     7321 ttttcaaaat atttaatgat tcaacaagtt ctttattttg tttttgcttt tctatttcag
     7381 aaatagatac atttaaattt ttcattttag gaatactatt atagcgtagt agttgttcgc
     7441 aaaataattt aaacgactct aattgatctg catattcgct tctcgtattg ttatctaaaa
     7501 catctaaatt accaacaaga tatcctattg atagatcacg tttagccttt aatgcttctt
     7561 caatagaagt attaagctta gaattcattc tgcggattga accttctaat acttcttcat
     7621 tattatttgg agctgattca ccaaataaat ttaaaatttc attgatttct attaaaggta
     7681 gtcctgatgg ctttttaata tatgagaatt tggttaactt ctccatagat aatactgcaa
     7741 attctgaaaa attcattgct gtatatgttg ttccatcaat attgacttca attttccctg
     7801 tacttaacaa cgcacctagt aaaacgacaa ctaattcagg ttccagatca aaggattttg
     7861 atagtctacg atcctcaacg ccacgaacag cattaatatt aaacaattca tcattgttta
     7921 atactttacc atttcctttt atttctaatg tttcaattat ccaacgtgca taagctgaat
     7981 ttgtaacaga ttcactagta attgtctttt gattacttag aagaactaag ccttctaaaa
     8041 ctgactcacc ttgaatactg tttctaccat ttattctttg taatgcgtca gaagcaacaa
     8101 ctttaatatt attactagaa ataaatcctg gtttataatt tctaaaactt gggtattctg
     8161 gatacttctc agaaaaatgt tctgcaagaa attcctgtgc agaaaaattg actaaagact
     8221 ttacattatc ctcttgaggt aagaacattc caaaatcatt taagtatcct gtttttcctt
     8281 tataagttat tttaaatgca tctacaaagc gttctgttaa ccatttgttt aattttttaa
     8341 aatagctatc tattttgtct ttatataatc ttttttgagc tgaagtcgtt tcattatata
     8401 actcgttagc tgcagcataa ttcaataaat tcattttaaa atttttatct tctaaactaa
     8461 gttcaaagaa tacctcatct gatttcaatt catctttata ttgaactttt tcaaacggtc
     8521 tcagcatata gacataaaaa tcacgttctg gttgagctgt agatctttcg ttaggtgacc
     8581 caaagaaaag gtatcctctt ctcattaccc ttctttgttc ccaaggtaac tcatgttgcc
     8641 aaatacgata gcctgaaacg taagtgttat catccaatgc aacagcattt tgtagaattt
     8701 tataatagta actatctaaa gtagcatttt ctatgatttc tgttcgctcg ctaattaaac
     8761 tatcaatatc aatatctttc tttaaatcaa gataatattg tccattctct tggtttactg
     8821 aaatatactg atagcttaca gtttccataa tcatttttat tgccacagac acactggttt
     8881 ctaaaaatgc tgatggatca tcgtcttcca ataaaaaatc taattgtcct ggaattacaa
     8941 gaaacagatc atctttcaaa gttgatgctg ttaaccccac tggactgtat atatcatcca
     9001 ttgttagcct attcaacgcc aacccattaa tgacttgaag tgaaacatct ttatacatct
     9061 gccctttacg atcactagga aaggatgaat tgactttatc ttttaaaata gctgtttttt
     9121 ctaaaacttc tcgaacagat gggatagttt tattagatgg atcctcttca ataaaagtcc
     9181 aataattatc atatgctact actcctgtac tatccactgg tacatcgtga tcaattattt
     9241 tctgaatttc catagtaatg gtttttaatg ctactcgttt ttcaatattg tgaactttct
     9301 caaaagcagt taagtaactt ggatgaattg gataaagatc cacaaactta tccatatctt
     9361 cagctagtct actgtataat ttagtgaaac tagttagatg ctccctaaca agagcttttt
     9421 gcttctcatc tttctttaaa agtctttcag atatgacata tgaaatatct tcttttacaa
     9481 ttcttacttg ttcaaagcgc tcttttacac gtctcaaaga atcagctaca aagctaaatc
     9541 ttgggttatc aaaaagcatt tcttgaatac ccgcaataaa tcgaaatctt gtattgtgcg
     9601 caatttcgcc aacttctcgt aaaaatccta aatctaaagt taactcttgc tcttttcttc
     9661 ctcttaaata atctagtaac tcatcaacta ctagtaataa acctttgtca ggatattttt
     9721 cattaaaaat tgacatcatc tctagtaaag aatttttatt actaacaatc tgatcggcat
     9781 taggaaattc gaaagtaatt cctagatcca ataaaccaag ttctaactgc tgtgttatta
     9841 tttcacgtaa tgacatttct gtactgccta tttcaaatcg aataacctta aatttacctt
     9901 caatttcctg tgctttttcc gcaacagatt cattttgaag gtgctgactt gaatctttat
     9961 actcagcaat tgtagagata acactcatta aatgagattt accacttccg taattaccta
    10021 caatcatcaa tcctttatta tctacgggtt tggaaaattg caactgatta atgatagtat
    10081 tattaattgt atcagccatt ctatcggata taacaaatgt atctaataag tggagagctc
    10141 tatctttatt gtttgcgtct gttagttgga tgactgaatc tatcggttca aaattaacta
    10201 aatctttata tttcattctt cattctccta attgataatc atttcatttt tatttatctg
    10261 tgcttcctga tattcaatat gttctgggac cgcataaact aatctattat tttcataaga
    10321 ataacgccaa ataacaataa ttgttttata acgactcagt tctctaaata attctattgg
    10381 tttaactttt actattggac taaataatat ctcaaaatta tcaataacaa taaccttttg
    10441 aggattattt ctaataactt cagtgataat ttgctgtgtt tttatagctt gtaatttaga
    10501 aggtatatcc aatagttgtt ctgatataag tctattgata ttaagtatag aagaatctat
    10561 ttctttaaaa agatatttaa tctcagtatt attattccat aacaaaacca tgaattgatt
    10621 gtagtctaat ttcttttctt ctactttttc ttcaagcatc tttactaacc attcaatatc
    10681 ttccatccag ccaccaccta taagatttac tttttaaacc acatgtactt attataacat
    10741 ctgaaacgac taatatagtt tgttttttga atgttacctt cttaaagcaa ttcaaatttt
    10801 tctgagtaat aatttagatg aaagcattct aatctatata tccagtataa tttttatcta
    10861 attcctatgt tgtctaatga tcttaataat aattcattat cataatgtaa cttttctact
    10921 tgctctctaa gtgtaattcc aaatatatat ctaaactgtg ctgattctga aatgatacta
    10981 ttatcttttg agatttctat ttcaagtttt tctaactgat acttctcata tgaataaatt
    11041 gaagatgaaa tactttttat tttatcttct gtaaacgtaa aatctttata tttatcactc
    11101 agtttttctt tttctttctt aatatccaaa tcaaaaataa aggaattact taactcggat
    11161 tttatcttga
//
LOCUS       DAPBBG010000078        11118 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPBBG010000078 DAPBBG010000000
VERSION     DAPBBG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.081"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            121..1314
                     /locus_tag="RD477_002516"
     CDS             121..1314
                     /locus_tag="RD477_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634832.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1480..1902)
                     /locus_tag="RD477_002517"
     CDS             complement(1480..1902)
                     /locus_tag="RD477_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634833.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            2492..2947
                     /locus_tag="RD477_002518"
     CDS             2492..2947
                     /locus_tag="RD477_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634834.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(3109..4281)
                     /locus_tag="RD477_002519"
     CDS             complement(3109..4281)
                     /locus_tag="RD477_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7634835.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(4487..5917)
                     /locus_tag="RD477_002520"
                     /pseudo
     CDS             complement(4487..5917)
                     /locus_tag="RD477_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(5914..7416)
                     /locus_tag="RD477_002521"
                     /pseudo
     CDS             complement(5914..7416)
                     /locus_tag="RD477_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(7413..9740)
                     /locus_tag="RD477_002522"
     CDS             complement(7413..9740)
                     /locus_tag="RD477_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7634836.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(10583..10876)
                     /locus_tag="RD477_002523"
     CDS             complement(10583..10876)
                     /locus_tag="RD477_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDT7634837.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         3096 a         2086 c         2055 g         3881 t
ORIGIN      
        1 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
       61 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
      121 atgacaacgt atcagcgaaa gttattatta tcgacatcgg cgggaattgc tttagagaat
      181 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
      241 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
      301 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
      361 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
      421 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
      481 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
      541 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
      601 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
      661 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
      721 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
      781 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
      841 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
      901 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
      961 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     1021 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     1081 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     1141 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     1201 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     1261 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     1321 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     1381 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     1441 agctttttca actgctaatt tccttctatc tggcggctac tattctttga cgtccgtcat
     1501 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     1561 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     1621 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     1681 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     1741 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     1801 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     1861 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     1921 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     1981 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     2041 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     2101 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca aaacgggatt
     2161 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     2221 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagtgaaaaa
     2281 attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
     2341 tactactcaa agcacaagtt cagctcaggc ttccagcagc agtaccacga tctccgaaag
     2401 tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag aagagaaaca
     2461 aaaagcggaa aaagctgcac tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca
     2521 agctgaagca aatcctacaa aagaaaacta tgcaactgct tcagcagcta tccaagcgct
     2581 acctgaaaac aaacaagagt tgacaagccg tcttgctgca attgattcca caatcaaagc
     2641 aaaagaagca gaagaagaac ggaaaaagca agaagaaatt gcacgacaac aagcagaaca
     2701 agcgcaacaa gaagcaatcg cacagcaaca agcagaacag gcgcgacagc aagcagcagc
     2761 tgaacaggcc tctcagcagg cacaagccgc agctcctgag caaaataatg aacaaactgt
     2821 ctatgtcaca ccgaccggtt caaaatacca tacacataaa tgtggaaatg gtacgtatac
     2881 ccctgccact ttaagcgaag ctcaagctcg tggtttaacc ccttgctcca aatgttatgg
     2941 aaactaattc aaaagttata ctataataat aaaattcttc tgaaacataa aaaaatagaa
     3001 gctggcgttc cagatagtaa accaaaaata gcagtttaca cgtccatttc gtcagcttct
     3061 ctcttttttc atttagtctg ttccctctgc ttttttggac atcttcattc attctttctc
     3121 aatcaaatag tacacctctt tttcttttcg cagtttatat cttccttttt tcaatttcat
     3181 tgaaaactgt ttcgaatgtt tatgttttac atggatttta agcaggagct cctcatgttg
     3241 caagcgatag ttcctgcctt ttaaatcaga aaatctcact agtccgtatt catcagaagc
     3301 tttcacaaat ggaacttttt ctctagtaat atagtgtttg ccgttgagcg tttttacaga
     3361 aaagaccatt ccttcgattg gtcgtccgtt tgtgttcttt acataaaaag ataaaggata
     3421 gtttcgctta gcgatggcca aagaatctaa ataagcgatg agtagccaaa ctaagccgat
     3481 tgatccaagt agtataccga taaaccaaat aaaaaatttc cattttttcc attgatcaat
     3541 tccttggatc gctttttttg cctcttttgc ttgataagga atacgatgcc ccgtcacaag
     3601 cagtcgatgt gtattgacca tataaggagt acaggtaagc aaagtcacga gatctctgcc
     3661 tttttggata cccaaagaat ctacttcatc aggtaagacc acttggattt tttctacttg
     3721 atacgccaat gtctttccat tgatctggat gaaaaactgg tctccttttt tcaacttagg
     3781 caaatctgta aataattttg cttctgggag tccacgatgc ccagaaagaa cggcatgggt
     3841 gtttttcccg ccagtcggat aagaactttc ttccaataat gacgttcctt tttgcaaaaa
     3901 tatttccgtt gtctgatcga atataggtaa gctgacattg atttttggga tttcgatgac
     3961 tccaagcata tgttccataa aaaaggcatt actaggtaaa tcttttaatg cttttgcatc
     4021 aactgcttgg ttgaaactgc ctatccctgg tgatacgttt tgctctgcca actgttggtt
     4081 tttctttgtc attttttcct gctgtttttt tatttcctct gaattcttat tcgatgcttg
     4141 cttttgataa tggatgatca tttgctgggc aagaaaatca ttcagtgaat cttctacaaa
     4201 aggataagaa aagatcccga tcccaacgac taaaatgatt gccatgaaga tgtccagtat
     4261 tcttattctg cttttattca tcatgtccac ctattttcta atatgtatat gaagaggttg
     4321 tgaaatcagc gccttgacct gagtattcgc tcaacaaacg gcaaaatagt tcctcctatt
     4381 ttgccgtttg ttgagcgaat accaagagac cgaccacttg tcacaacctt tctccccatt
     4441 tgaatcacta tcgtttattt atcgtatgat ttttgtttcc aatagtttaa gcttctgttt
     4501 cttgatggcg ttttttgaag tagatcactg ctcctccaac taaagcagta cctaccaaga
     4561 tgaatacgat gatccccata ccaccagttg aaggcaagat tcctttaggt tggttatcga
     4621 tcgttgtttc atcagaagtt gttggatcat catctactgt aaattcagtg tacgcagctt
     4681 cattcttagg taatgcgtag ccttctggtg cttttgtttc ttgaaggcag tattgcccct
     4741 tgtttcagcc cttcgattga aaatttccct tctgaatcag aaatcaattt cacttcttcc
     4801 cagtcagaac ctgtgacttc atctgaccaa gcatatgggt attctcctga ggcaagattt
     4861 gctatttttt tcattaattt aacagttgga tcttttgctc ttagaataga aaattctgca
     4921 tcggctaatt tttcttgcgt gttcacatct tgtttgatga agtggaaatc accgtagttt
     4981 tggacatttt ctacagtaat cgtgttatcc gtagttgtat tttcccgatt gactacaccg
     5041 tttggattcc caagattggc ttcatctgcg ccatggatga taccggttgt ttgttgcaat
     5101 tttccgtttt ttactataaa agtagtttct ttttgtggat tggttaattg ataaccatct
     5161 ggtgcttttg tttcaactaa agtataattg ccgtctatta atccattgac actgatggat
     5221 ccttcagtat cagttgtgaa tgtcaacgct gactcagcag gatcatagag agcgacacca
     5281 gtgttttcat caaaaccggt cacgaattga ttctctgaat ttttgatctg gaattgcgcc
     5341 cctgctaact tatctacccc tgaaaagttc ccagctttct ggattttgat attgctttct
     5401 ttgatgacat ttttagcata taggtgaatc gtgtctaaag gtttgtcagg gtctgatgaa
     5461 ttcgggttat aaaagggtaa gcttaacacg atatttggcg acgtttcgac accgggcttt
     5521 ggtgtttcta caaagacgta tacagcatct ttcccatcag atttacctgg aagagtcagg
     5581 ttagctaatc cttcaccaga agtcgttaca cttgcgaagg gtgtagcgtc tgctggaact
     5641 gctgtgtctt tacttggata gtacgtagca gtgatgtcat atacgttgaa ttcaacgccg
     5701 ttcaacggtt cgctattcgg aaatgtcaat tccgttccgt tgtttatttg attttctggt
     5761 gctgaagtga atttcaattt atgcaaatta accgtaacat tttctggtgt aggtgatacg
     5821 gcctagaaac ctctgcagtt cctaggccga gaacgaaagg aagcagcatg atagcagttc
     5881 ctaaaaacgt ccatattttt gttttgatgt ttttcatttt atttccctcc tattgtttcc
     5941 tttttgttgt tttttataaa accaagctgt ccctgttgaa aggactagca acaaccctaa
     6001 aattgagaaa cctccaagca tctcttcgct tgtttttgga aagcttcctc catttttttc
     6061 atttgatgga ccatttgtct gcatctgaac tggcgttttt ccattattcg tcggatttgc
     6121 ttttggttcg ttgatgattt ccaatggtat aggatgaagc ggatcataac tgcctttttc
     6181 aacttcaaag agaatggttg attgactcaa tatatatcct ttaggcggtt taatctccac
     6241 tagttcgtaa cttccaaaag acaatccatg gacagaaaat gttccatctt tactggaagt
     6301 aaaaatcgtt gctcttgaat catctttggc tacccaatga tttatcccgt tttcttgcgc
     6361 taagtattgg tcattttttt tgactagaaa ttgagcatct gctagttttt gtttcgtttg
     6421 ccgatccact ttgacaaatt gtttgcctcc tgtttggatg gcaagatgtg tttctactgt
     6481 ttgattgtca gtcgtcaaac gtacatcgtt atcaaatgtc gttttttcac tgttttcttt
     6541 caatgtcatt tggtaacgga tcgttaattt tttcccagca aacgaactaa gatcttttcc
     6601 agaatctgat aagacaaaac catgttttgt tttttgtgtg tgaaatgtat gaatcttgtc
     6661 tccaccaatc aaaaaatcga tcgtttctgg ttctagccat aactgaggat cagctgtatc
     6721 tttgatcgta taggttccta tctcgttgat gttctcagga attgttacgg tgatgagata
     6781 agggacttta tccccatctg caaaatctgt ctgcttattc tctactgttt ttgttaatgt
     6841 gagtgcagca ggtatttttt gttcgctctt tggatacaag tggatggtcg tcaatttatg
     6901 attagatgaa tctgtcacag gaagcacaac gacaaatggt gctgattttt cttttactta
     6961 atctgaagtc tttatttccg caaatcggta aacagcgtca cgaccttgct catctttgtc
     7021 cgatgcacta aaagaagcaa tgccctcttc ctcgtctact gtttttgtca cattttctgc
     7081 cagtattttt tcactatcac ttttttgtgc tagttttctt tgtgcgtctt ccacactact
     7141 tccttctgac cgcagcttgt aaaattcatc cgagacatca tatatagcga aagtaacgcc
     7201 gttcaaccct tttactcctt gaaagttatc cgggatattt attccgtcgt tttgactgat
     7261 acttggcatc ttatcgcttt cgaacattcg attatgcaaa gtaaatgtaa taggtgattc
     7321 tctagctgac acagtcggag aacctataaa aaagaccagt aggataaaaa agagaacagc
     7381 aaatatatcc acacgtacat tttttgtttt tttcatcgct gttcacctgc ttttttctta
     7441 cgatggataa aataaaagaa gccagaaaaa ataaagatca gtcctaaaaa gacaaagggc
     7501 agtagaccta tcccacctgt cgaaggaagc aagagagtcg gattgatcgt caacttagcg
     7561 gtatctgttg cgataatcgt ataattattg tttacacttc cttcgcctaa actaatcgtt
     7621 actatttgtg atccaatatc tgtccggctg ttaactgtta gggtataatc caatgattcg
     7681 cctaagacaa caccggtaac gtggagattt ccaaccgaaa tcgagtgact ttgggcatca
     7741 taattaaagg cagcgtcatc gacagtaatc actgcgttag ctttatagat ggtataagtt
     7801 cctgatgtgt tggctatatc agtaaatgac aacttgggat ttgctgcttt gactttgtct
     7861 attccgctat tcgttagttt aattgtatag ctgccagcat taaccatatt ttttgctgat
     7921 tgtgcatctg ctgtgtaatc catactgcta agtgccactt gtactggagt aagcgaacta
     7981 gtgacaccct ctgctagctt gacatggatt gacgggacac ctgcttttcc atcatatgtc
     8041 ttactgccac tgagctctac cgcaccgctg atttgcttaa tcgtatagac atcagatgtc
     8101 tgcacgatag taaataaata gtttgttctg gcagctagtt tagctagtcc gctttttgtc
     8161 aattgccaag acgtataggt tccaatttca gtaggcgcag ttacttcaac tcccttttca
     8221 ttcatcgctt tgaaatcagt tggattccat gtgatttgat caatactgag atcattgttt
     8281 aaaagggatg ttgattctgt tttatcatta aaggcatatt taacgatcgg tacaacagaa
     8341 agaccatcat agtacttact gccatttgca ttgattgata ctgttgcatt tgtcttataa
     8401 tataggatga cttcagaaat ctgttttcca tttgcgtcct ttacgattac atttccattt
     8461 gtatctaaac tagctaaaac actcattgct gattggttct ttgtttctgt cgctgaaaca
     8521 gctgtaaagg tatatccgtt gatagtaggc gtattagtcg tcaggtcttg tgcagcactt
     8581 ccgtaagtaa gattcattat tttagaagga gcaatcatct gtccaaaata gtcttgatac
     8641 ttaatagtga ttggtgtttg tttgcctgta taggtcaccg tatcgacgtt actttctagg
     8701 gctgtgaaag aaccgtttgt ttgtgcaaaa gaatagcccg ttactgctgt tggagtgata
     8761 accactttgt cacccacatg gattccagat aatgtagtcc cgtacgttcc gatttttgct
     8821 gctccaccat tgactgtata agaaaaagtt cctgtccgat tcgcattgac ctgaaccgtt
     8881 gctgcttgat aggttggaaa atttgaaaca tttcccttac catcaacgat ctgcccattt
     8941 ttgatcgagt accccgtgaa tggaatatca tttgctaact gtataccgat tgaaaaagta
     9001 ctcatacctc ctccggcagg aagccctccc catgttgagg gtgtaaaaga ataactttta
     9061 tccgcattgg gtgtaactga atcgatcgta tttccagcgg tagccacccc catatagata
     9121 ctttttggga atgtgatagt agtaccattt actgtggagc caataaacag attatggata
     9181 tctggacggt tatcaataaa agcgattgct ttggttacgc cttgtaacgc agttttatcc
     9241 gcactcatcg atccgttaac cgcaacaatg actccatctt tggcatatat cactgtatat
     9301 ccatctggta tggtatgtcc gttgaaattc gcagtggccg caacagtact aaatgtctgg
     9361 cttctacctg tttctgaata agctttctcc cctgctgctt ttgctgctgt gatttctttt
     9421 tctgtcggat caatcagttg agtcgaggat gtggttgagg ttgtctcgtt gttgctagac
     9481 tgattggtgc tttctatgga ctgtatttca gaatagggtg gactatttgt ctcctctgtc
     9541 gtttggttag caaaatttga cgtttctgta taaacgttta cataaatact tgataagctc
     9601 cctaagaaag taaaaatcaa gatgatcgaa atcataatgg gatgtaagta ttttctttta
     9661 gatacagctt tgagtctaga aacaaaatgt ttttgacata tacgttggat tttattttga
     9721 gttttcttaa tcattttcat ccctcctctc aataaaaaat catcaaataa tcctttacac
     9781 gttattctaa atttaatttt tattatttta ttgatgtata gaaagtttgg ggcttttttt
     9841 ctctttttat tctaaggtta ttttagtctt gtcctttaat acaaagattt tatttttaaa
     9901 taaaaaatac tcttttttta cgattattga aaaaaaaaga taaaacttga ttaattgaag
     9961 tattgcttaa atttggaagc tatgagggaa gaataaaaga gttaggacgg gattatctac
    10021 tatttttctt tgttttaaat atttgttatc tttgaacata aaagagaaat gtgtcgactg
    10081 cagtgttgtg tccctcatca cgaataaacg gtgttcaatt agctgatctt gtattcactt
    10141 aaaaaatggt aaagtacgag ttgctattcc ccattttcaa ttttctaacg ctcaggacaa
    10201 aatactgttt tcacaacctc attggaataa aagcatatat attgtttcaa gaacgaaatc
    10261 ttgttatctt tttaaagggc atctgtccaa aatctaattt taggaggtac gttttggtat
    10321 ttattcgtac aaataaaaga gaattatctt tattcatgta acgcatccaa ttgatttttt
    10381 catgtacgct cctggaacga ttttactgac gattgcttat ttaactgcga atcgctcatt
    10441 agcttttatc atagctatcc atattttgaa taacgttctt ggctttgtat tatagataca
    10501 gatgacaaaa aaagttcaag tatgcggtgt agaaccaata tcgcatactt gaactttttt
    10561 caatgagtat aaattaaaac ttctaatttt caattcccat catctgatat gcccgaccgt
    10621 ctgaacttct tttgaaaaag ccaaatgtga ttagataacg tctttgttcg acataatcgc
    10681 cactagaaaa tacgttcagc agtatcgaat taacctctgt ttctgtataa gtcactccat
    10741 ggtcaaagcg tttcaagact tctttcaata ggatgtattt cttttttgtt gaacttggta
    10801 cttgttttac ttttccatca ttcatgaatg tgtctaaaac tttcatcttt tcttgctccg
    10861 tataagtata aaccatcttc tttctccttt tgattctaag aatactccat ttatgttaga
    10921 atattctgga ttttttatta aagcggttac tcttaagaaa gatattatca tagtgctctt
    10981 ttttttacta ctacttatag gtagatttat tctaatccat tattctcccc taccaataat
    11041 taaagaaaaa actggatcag ggtgttcaaa ttacatgcta agaaaaaaga acccttgaat
    11101 accaagggtt ctttctca
//
LOCUS       DAPBBG010000079        10562 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPBBG010000079 DAPBBG010000000
VERSION     DAPBBG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.082"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(184..1128)
                     /locus_tag="RD477_002524"
     CDS             complement(184..1128)
                     /locus_tag="RD477_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDT7634838.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1262..2653)
                     /locus_tag="RD477_002525"
     CDS             complement(1262..2653)
                     /locus_tag="RD477_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDT7634839.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2779..3189
                     /gene="tagD"
                     /locus_tag="RD477_002526"
     CDS             2779..3189
                     /gene="tagD"
                     /locus_tag="RD477_002526"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDT7634840.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3365..3778
                     /locus_tag="RD477_002527"
     CDS             3365..3778
                     /locus_tag="RD477_002527"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7634841.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3935..4192
                     /locus_tag="RD477_002528"
     CDS             3935..4192
                     /locus_tag="RD477_002528"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7634842.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4279..5517
                     /gene="istA"
                     /locus_tag="RD477_002529"
     CDS             4279..5517
                     /gene="istA"
                     /locus_tag="RD477_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDT7634843.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDEI
                     RAYTRESLKQYDLVEVADEE"
     gene            5507..6247
                     /gene="istB"
                     /locus_tag="RD477_002530"
     CDS             5507..6247
                     /gene="istB"
                     /locus_tag="RD477_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDT7634844.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            6657..6821
                     /locus_tag="RD477_002531"
                     /pseudo
     CDS             6657..6821
                     /locus_tag="RD477_002531"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6888..8315)
                     /locus_tag="RD477_002532"
     CDS             complement(6888..8315)
                     /locus_tag="RD477_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDT7634845.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(8312..9454)
                     /locus_tag="RD477_002533"
     CDS             complement(8312..9454)
                     /locus_tag="RD477_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDT7634846.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            complement(9458..10453)
                     /locus_tag="RD477_002534"
     CDS             complement(9458..10453)
                     /locus_tag="RD477_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7634847.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT         3649 a         1888 c         1603 g         3422 t
ORIGIN      
        1 tgatcattaa gtttggcttt tatgtcataa ttgaatttta taaagttctc tttttcatgt
       61 aaaatattta attatatatt tcctaaataa aaagtaaaac aagaattaaa tagttggtta
      121 tacaacttta attcttgttt tactttttag aattatttat ccaaagatgc aatcattttt
      181 tcatcaaatt tctgtcttta aataccgttc cattccgctc aatatatcaa atttaggctg
      241 atagcctaaa gaacgaattt ttgaaatatc cgctaaggaa tcacgaatat cccctgaacg
      301 ctccgcttca tatttaagcg ctagctctgt tcctaagatt tgatcaattg aatggatcaa
      361 ttcaagtagc gtagtgcttt ttcctgtacc gacattgaat tgctgtccta aagctttttc
      421 ttccttggca acaagtaaaa gtgcttgtat aacatcttct atataaacaa agtctctaga
      481 ctgactccca tcgccaaata aagtaaattc cgctttttct cctgccaatt gttttttgta
      541 acgatccact agaatcgaaa tgactccgct atatggagag tttggatttt gatttggacc
      601 ataaacgtta aaaaatcgga cagcactagt cggaacatca tataaatgac agtaatccaa
      661 aacatattgt tctgctgcaa atttatcgat agcatatggt gttaatggac ggatgactga
      721 ttcttctttt tttggcagtg ttggttcatc accataaact gcagctgatg aactaaatac
      781 taatcgtttt aaattagatt gatatttccg tgctaactca agcaataaaa gcgcactgtc
      841 aaaatttaca cgatgtgttt ctacaggccg ttctacagaa tctgccacac tagctacagc
      901 agctagatga aaaatatagg caaactgata ttctttcatg attctttcca tcaattgaga
      961 atctgataca tccccctcaa tgaaagtcac ttgtttatcc atatccaagt tttctttttt
     1021 tcccattgat aaatcgtcaa ctactacgat tttgttttct tttcccaaat aattcgctag
     1081 cgttgaaccg ataaaaccag ctccgccagt aatcaatata gtgtccatca aattcactcc
     1141 aattgcacat tttcgtatca aataatatta gtttaccatt tttatattgt aaaaactata
     1201 aaatttgttg aagttgggat aactagtttt cttttctact ttcatatacc taaaaatagt
     1261 ttcaattagt tactccttct tctttagata tagttttttg gatagaaccc agatatcccc
     1321 aaaaaataaa tgaacctatc gtactaacca aaatgagttc tgtgataaat aatgccgaaa
     1381 ctgctattgt aagaatcatt aaaatagcaa aaaagtgctt atcttttact agatttggtt
     1441 gtgtgaaaca ataacgcaag gctttaaagc attggataaa taagaaggtt aacaatgtca
     1501 atagacctaa tcctcctgta gcagccatca ctaagaaaat aaaattatga gcagttaaag
     1561 tatcttttcc cattaatgta tatggtagtt tatcatgtgc atattcaata tagtagcctg
     1621 gagaacctcc ggatatcggc ttactcttga atatttcaat tgcacttttc caaagttcaa
     1681 accttaaatt agaaacatcc ccgctatcag tgtcagggcg ttttaaatca actttattgc
     1741 tgctagttgt tcctgatgaa tcagatgtat ctttttttac ttctgtaata tgcggaacat
     1801 gtgcccatgc atgagcagtt atatctgtta cataatacat cgcaattata cttagcaatc
     1861 ctactactaa tgcagttagc catcttttta aagtattagt tttaagaaaa cgactactat
     1921 aacttgtaaa gaaactaaaa acaaaagttg ttattaataa acaaactaat gtggtgcgtg
     1981 aacccgataa agcaataaac gagaattgaa taaaaatatt aattacaaaa aatattttaa
     2041 atagaagatt taaatgcttg taagtgacca aataatatat agcaataatg atgacgatta
     2101 atgatataac cgcaccaaaa tttggatctg tatatacacc aaataaacgg ttgcctaacc
     2161 accctaaacg aactgggtaa tacttatatt ttattggaat ttttatgcca tattgaatga
     2221 caaaccagta taatgatact aaattagcta aaaaccatac caatgaaagt atccaggtaa
     2281 atactttgaa aaaaaagtta ctactatagt ctttgccaaa ctgataaatc ataaagaaaa
     2341 agattaattg ccacatgatt aacttaatat tttcgctata tccatatttt aaattcatta
     2401 aactgcttac tagcatcgcg ccaataaaag caattaatag ccaatcatat ttttttagat
     2461 ttttttctct aaatgcaata attgcatcta taatgatcaa gacaactccc gctattccag
     2521 caggccaaaa aagtaaagta tcatatctag taggtaatag taaatacggg cctattgctc
     2581 gtaacattaa aataaaacca aaataaataa aataccaacc tttcacatat tttaaaaatc
     2641 tttcattcaa catttttcct ccatccacct atttctcata aatatttctt atccttttta
     2701 ggataaactg gtcctttcaa ttctaacaaa aaaatgctaa actaacaatg tatattaatg
     2761 aatttcgaga ggtgaaagat gaaaagagta attacttacg gtacctttga tttgcttcat
     2821 tacgggcata tcaatttact gaaacgagca aaacaatacg gagactatct tattgttgcc
     2881 ctttctacag atgaattcaa ttggaatgcg aaacaaaaaa aatgttattt tagttatgaa
     2941 aaaagaaaac agttacttga agctattcgc tacgttgatt tagttattcc tgaagaaaat
     3001 tgggaacaaa aagtaaaaga tataaaagaa taccatgcag attatttcgt gatgggcgat
     3061 gattgggctg gacattttga tgaacttgaa caactaacag atgcacaagt gatttattta
     3121 ccaagaacac ctgagatctc tacaactcaa ataaagaacg aacttaattc tttaaatagt
     3181 gataaataat attttttaaa caataaaaga acaatatatc tggataagac gtaaaaaaat
     3241 ggctctataa tttctaggac tgatagaatt ttctgtcggt cctagatttt ttgtaaaaaa
     3301 aaacagacat aaaaaaattc cgatagaata gagtcgtcta aaacatcaat ctgaaaggaa
     3361 gtttttgata tccatttctc attctatcaa agagctcttc actattaaag ataaaaatat
     3421 tgaaattaat tcttttcaag aaacttttta taaggacaag aaagccatcg taatcgaagg
     3481 tccattagtt cgcactttta gacattgtct attttgtaaa gaatctgcaa agcagatcga
     3541 gaaaaacggc aagaagattt caatgattat cttaaatcgc agtgggaata aacaaaccta
     3601 tttgcgtctt agaaaacaac attaccactg ccaatcatgc gggaaatatt ttactgctca
     3661 tacgtatctt gtcgctcctt catgttttat ttcgaaacaa gttcattaca cgatattaga
     3721 agagttgacc gagcgtcaag cattaaagac aattgcgaaa cgttgtgatt ttcagtaacg
     3781 acagttcaac ggacattatc tgctctatcg gcaacgctgc gacagaagct agactattta
     3841 cctcattgtt tactattcga cgagtttcgc tctctttcaa ttttccatgg gaagttctcc
     3901 ttttcttgta tgaattgaga gacagggaag ctctttgaca ttttgtccag ccggagaaaa
     3961 aaaagatttg attgcttatt tcatgcgttt tacaagaaaa gctcatatgg gggttcgata
     4021 cattgtcaca gatatgaatg ccccttattt ttcattagtc aaagaaccct tcccaaatgc
     4081 acaaatcatc attgattgct ttcacattgg gcaacacttg aacaagtttt tcgatcgtgt
     4141 tcgaaaacgt gtgatgaagc agttgaatca aaaatgtcaa cggcaccttt aaaatgtagg
     4201 aaaacggcaa tttaaaattg ttggtttacc tacatttttg tatgctcttc ttatcccaaa
     4261 tgaaatggag gctcagggat gagaaaagac atacgcgaag gagtgatgat ctacgtgatc
     4321 aatgaaatca aacccaatta tgctgcacta gcaaaacaat acgactgcga ctaccgaaca
     4381 gttaagcatg cttacgaaga agcccaagtt aaagaatcaa aacctccaga acgtaagaag
     4441 cgtccaagta agttagatcc ctacagagaa atcatccaag acaaaatcaa agaccaatgc
     4501 agtgcttaca gcattttcaa gtttattgag cacaaaggat ttggaggaag ctacagttta
     4561 gtaaagacct attgtcgcca gattaagcag gcgaaagtac acaaggcaac cgtccgagtc
     4621 gaacatagcc ctgggctttc tgctcaggtt gactggaagg aagatatgcg actaatcagt
     4681 aatcagggcg aaatttttga gttcaatatc tttttgtacg tcttgccata ttccaagaaa
     4741 aaatatgtca cactaacttt cgaccgaaaa caagacacac tctttgagtg cttagatgat
     4801 gcgttttatc aaaccggtgg cgttcccaaa gaaatctggt tcgataatat gaagacagtc
     4861 gttgaccatt ctcgtagtca gttttccaaa ccgcatttca acaaacgttt ttatgctttc
     4921 agtaaggatg ccggcttcac accaattgcc tgtcggccat ttcgtccgca aacaaagggc
     4981 tgtgtcgaag ctttagccag aactgttgac cgcctacggg tctataatca tgaattttat
     5041 gatgccgttg agctgtgtca tttagtgaat aacctttgtg ttgagttaaa taacgaggtt
     5101 tctcaatcaa ctgatcaaac accagatgaa cgttggcaga cgaacgaaaa agagcacctg
     5161 catccatacc aaaaaaacct tttgaatccc tattttgaag actttattcg ccgtaaagtc
     5221 accaaggaag aaatggtcat tttccgtaaa tgcaggtact ctgtggcgcc tcgctatatc
     5281 ggtaaagaag tcgaaatcga actctccgat aaagaagacc aagtacaaat ctattataac
     5341 ggagaactga ttcggtcgca tccattaacg acaaaacagc tgaactacca cgaggaagac
     5401 tatttccaaa tcttgaaatc cgatgtcatg agtcacaaag aagacgatga aatcagagcg
     5461 tatactcgtg aaagtctcaa gcaatatgat ctggtggagg ttgccgatga agaatagcca
     5521 ttaccaagaa ctgatgacca acttagagac tcttggtttg aatcatatgc gggaatatgt
     5581 tcctaactac atcgaagtgg ccaatcgaga ggagctttct ttaacggaag ctcttctcga
     5641 gctgacacgc agagaagttg atcatcaaaa taagcaaaaa atgaatcgtg tggtggcacg
     5701 cgcccgcttt tccaagagaa ccagcctgga agagtttgat tttgccttcc agccaagtat
     5761 caataagcgc gaagtgctag acttaaggca catgggcttt gtggaaaaac atgagaactt
     5821 ggtttttatc ggcagtcctg gtgtaggcaa gacacatctg acgattgcat tgggaatcga
     5881 agcctgtcgt caaggctatc ggacactatt catcacctgt catgagctgc ttttgcgtct
     5941 gcgaacagcc tatgaaaaag gtaccctaga gcgtgttctc aaacgatacg ctcgctatga
     6001 cgtcttgatt attgatgaga ttggctatct tccgattcaa aaacaggaag ctgatttatt
     6061 ctttcagctt cttgccatgc gctatgaaca ccgctctacg atcattacga cgaacattgt
     6121 cttatctcgt tggggggagt tgttccaaag ttctgagatc gcagctgcca tccttgaccg
     6181 tttagtacat cacgtcaagg tgttcaagat cacaggcaag tcttatcgta tgagagacaa
     6241 gatctaaagt gtcgattgtc gacaattact attgccgaaa acctacattt ttatcctgcc
     6301 caaaacctac aattttaagc tgcccttgac atcaaaaaga cacttcacaa gctaagtgct
     6361 atcgacaatt gaaacctttg tataaattac ttctataatc agatgacgag ttggattata
     6421 caacattcaa gaaatggcag aattttcaat ggcgctattt aacagaaagt gaagtagttg
     6481 attgtctacc ctcaatctct tatgaactta aaatggctta gcagtattat caaaagctgc
     6541 ttaacaatta tcacaagaaa cagctagcta cttcgagaaa tgaccaaaga agttccctcg
     6601 aaacttcact atcttaaaaa gcctttttca tatctgaaaa aggcttttaa ctagcatttt
     6661 cgaaaaagta ttttaataca aaattagaga atcttcgcac acatatcaaa acattgaaac
     6721 gatttgctta tggattttgc agttttacca atatgcgctt gtggattttt ctaatcaacg
     6781 ggttaataag ctatgcatta aaagctagta aatcttacta actttgttga tcataaatga
     6841 ccttatcagc ccgatttgac aaagaactac ttttttacaa cattatttta ttttcgaata
     6901 atctttctta cttcatttcg tatatctgga aaaagaaaat aaacaaacgg aagataaata
     6961 atgaaaatca ttattgattt aattgccata tctaaaaatg aagaagagaa taattgtgga
     7021 agaaagtaat aggccgaagt agtcaaagta agattgacta taaaaaataa gacaacgttc
     7081 ttcccaaaga aataggatat ttttatttct tttcgtacaa aaaatactct aaagaaaaag
     7141 actaataatt ctactaccac gagtacgatt gtagccgcta cagcgccata agtaggcaca
     7201 aaagctacat ttccaataat acttacaatc gcaccaatga tcattggata agcatactcc
     7261 ttatcttttt ctatagcaat tgcaaattgg ttagcaaaaa ctccaccaat tggactaaca
     7321 actataatta gacaagaaat aagcatgttt attgtcatcg gtttgaaatt ctccccaaaa
     7381 aaccatggaa caaattcttt tgtattgata aacaccacac taaaaagaat taatgcaata
     7441 ataatcgtgt aatcaaaaga tttttttgtg taaactaaaa tattttctga ttttccctct
     7501 gcctgtgctt tagatatttt aggcaacata acaatagtaa ttgatgttaa gactgccaat
     7561 ataatacgag caattttttg agactgatca taataagata atgctgttgg atccacaaaa
     7621 tacccaacta tcgttttgtc taaagttgtg tacacttgca taaacaactg aggagtcaaa
     7681 ataataaaag ctggtttcca caactgttta aaaacattag agttaaagag tggtttacct
     7741 acttttccgt ggatactcaa ccagaaaact aaacttccta aaaagttagt aattgaagta
     7801 atgaacacat aaatccacaa atcatcttta gttgaaacaa aaaggattat taacaatacc
     7861 gatgataatt tgataactgt atttctaata accactttcg aaaattcttc aattccttga
     7921 aaaaaccaag aaatatctaa tgttagccct gcaataaata tcaattgaat taagtataaa
     7981 tttttatact ttacatcaaa aaaataaata taggctatat agagaaagga aacactcaag
     8041 cctattaaaa attgaagaaa gtaaatattc cagaattgta aagatagtgc ttttttatct
     8101 ttccttgatt tagcgatatt tcgaatacga aaaggattca ttcccacagc aatgacagtc
     8161 attaaaaatt gtgatattga gaaaacatac ccataaatcc ctaaaccagt tgaacctatt
     8221 ctacgactaa gtataggtat agtaagtaag ggcaaaacaa tcaaaaagac ttgatatatc
     8281 gcattataaa atatatcttt tactgttttt ttcactttat atccctctct aacaactcaa
     8341 gaaccttttc tgtacttgcc cctgtttcat aatttccaat ataatcaagt aactcttgag
     8401 aacttttcaa aagcttttct tgttggtttt taaaaacatc aacaaggcca taactatctt
     8461 gtacgcttat aattggtaac tcacttaact gtatataaaa acctctttct aagctatatt
     8521 ctttttcatc atagttaaaa agaataattg gtttttttgt tagcgcaaaa tcgaaaaaaa
     8581 tagatgaata atctgtgatt aaaacatcta tctcttcaaa aagcatttca agaggaatcc
     8641 catcattaat taaagtaatt tgatgttttt tcatatattc tatactttta gaatcagttg
     8701 ctaatttact atgaacatta ggatgaaatt tagtcattaa atttaattct tgttgttgct
     8761 ttttaaaaaa ttcacgtaag ttatccacac ctaaccattc tagttgcgta attccgttgt
     8821 cctcaatatt atttcgaaaa gttggagcaa ataagacatt gatttgtttc tttttcaagt
     8881 caaacacttg ctctttacta gaggctaatt catcgtttct aggagttcct acttctaata
     8941 ttttagaagg aacgatattg aatgctgaaa catacttctc agaactatag cgattaccag
     9001 ataataaata tgtaattgac tcactatctt ttttcgcata gcttaggtaa cgtttaggta
     9061 acttatcttt tgcatcaaat tcaatttttt ttaaaggcgt accgtgccat gtttgaaaat
     9121 aaaattgttt tttccttttt gcatacttaa tcgttttccg actatcgtct atccaatact
     9181 tagctgtata caaataaaag gcagctaaaa aagaatcgga atcaattctt ttaatataat
     9241 ctggtaaatc agttataatc gcttcttttt tgattaacca aataattctg taattcaatt
     9301 ttttttcatg aattttctct gaaataactc ttggatttcc agaataatta cgcccgccaa
     9361 aacttgaaaa tacaattaaa tttttttgaa taggtaatat tttaataaaa accgggaaaa
     9421 tattccttga aaaaaatttt tttatattct tcatctctta cctcacagat tctatcttaa
     9481 tcaaaaattt acttgctatc ttactacttg acatatagta aggcaagttt ttatttatta
     9541 ataaaaactt tactaatttt tttaattttg gtgaaaagtc gggatcaatt ttcacttctt
     9601 tttcaaagcg ttgtaaaata tttatagaac gtaaataatc ttctttattg gtatagttat
     9661 ttttaatcat tgtcatcatc atccttattg cttcatagac atagcgagtt ctaacattaa
     9721 ttgataattc cggataaaac ttatctatga atactagtat atcttcatgg acttcaaaag
     9781 aatcaaaaat tctaggattg aagcttcctc tcagtatact tttagggctt tggtaatagt
     9841 gatatttagc agatgaattg acagcaactt tttcggaatg atcgatcaac aaataagtag
     9901 tagccttatc ttccattaat ttacccagtg ggaatcttat attatcaaat aaatttctat
     9961 tgaataattt attatttgca gaatagccaa ttttattatt tataaataag tttttcattg
    10021 cttgttctct atcaaatatt tcccattttc tctcagcaat tgtatgttct tttagttgtt
    10081 ctaaacttga aatctttttt atttttagca ccgagatatc tgctccataa gtttgcgtac
    10141 caaaatatag taactcaaga tagtctggtt ctatccaatc atcgctgtca ataaagcaca
    10201 caaaatctcc tgttgcagaa tcaagaccag aattcctagc cgcacttaaa ccaccattca
    10261 cttgatgtat cacttttaat cgcatatctt tctttttata ttcttctgca atttccccac
    10321 ttcgatccgg ggaaccatca tcgactacta ttatttcaat atttgaatgt gtctgattgg
    10381 aaatagattc taagcagttt tctaaatagt tttctacgtt atatgcgggt actataacac
    10441 taattttatg cactctcttc acttcctaaa aatcatcttt tttattttta caaatgtatc
    10501 attaccagta acaattttag tcttccgaag ttaataaaca taaaaaataa catatcgaaa
    10561 aa
//
LOCUS       DAPBBG010000080         9813 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPBBG010000080 DAPBBG010000000
VERSION     DAPBBG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.083"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(128..778)
                     /locus_tag="RD477_002535"
                     /pseudo
     CDS             complement(128..778)
                     /locus_tag="RD477_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            1103..1519
                     /locus_tag="RD477_002536"
     CDS             1103..1519
                     /locus_tag="RD477_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7634848.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1516..1986
                     /locus_tag="RD477_002537"
     CDS             1516..1986
                     /locus_tag="RD477_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7634849.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            2000..2773
                     /locus_tag="RD477_002538"
     CDS             2000..2773
                     /locus_tag="RD477_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634850.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2787..3599
                     /locus_tag="RD477_002539"
     CDS             2787..3599
                     /locus_tag="RD477_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7634851.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3653..4618
                     /locus_tag="RD477_002540"
     CDS             3653..4618
                     /locus_tag="RD477_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7634852.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4755..7478
                     /locus_tag="RD477_002541"
     CDS             4755..7478
                     /locus_tag="RD477_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7634853.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7491..8123
                     /locus_tag="RD477_002542"
     CDS             7491..8123
                     /locus_tag="RD477_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7634854.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9022..>9813
                     /locus_tag="RD477_002543"
     CDS             9022..>9813
                     /locus_tag="RD477_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7634855.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3307 a         1503 c         1904 g         3099 t
ORIGIN      
        1 aacagcgaca ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct
       61 gtcctctctg tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa
      121 cagaaccgcc aatcatatgg aagccaccga ttaggtattt tatgggttta gtcggaaatg
      181 tttcaagagc aatttccatc atatcgataa tgccatgatg actacagcca gtaaagacaa
      241 tcagaccgtc ttcttgctca attacgacca ataattcatc tgaaaaatca tcataaacaa
      301 atttatcatc catctttttg tataaattct tggtggtcga gtttgctttg cctaggggga
      361 tgatatggaa gctcggagca agttctgttt tcgtttttat tgtatgtaaa ttctcatgca
      421 ttttaaggat ttcattttta ggaataccaa catacagaaa cgtggttaaa atctttaaat
      481 aatggggtaa agttacacct tcagctgtat aaataggggc gtttcctgat tttaaatatc
      541 ttaacccgcc ggtgtggtca gaatgaccgt gtgaaataat cactgcctcc agatcattca
      601 aagtttcccc cattttagtc gcattataga ctaatttttt attagcgcca gcatcgaaga
      661 gatatttctt ccctgagtag ttaattaaga gagataaccc atgtccactt ttaaagttca
      721 aatttattct tgagttttca aataaaacct tgatttccac tataacatct cctttcacat
      781 gaacgtgtat tcatatattg tagcacatta agttctattt tttgttgtaa ttaccgaaaa
      841 aaatattaat ggacgctttt tacgctttat ttcgtatgtg gaagaaaatt tcttaggatg
      901 tggcggggga ttggggcaaa gctaaattcc aattacagtg atcatttaat tagacacctt
      961 taaataccca cactttcatc aacactataa tttagggaaa gaaaagtgtt ggcatgatat
     1021 agaaaaacct ttaattaaca gcgtttccta aaattggcac gcatcttgct tatagaaaag
     1081 cgtaaggata ggaggaatga aaatggcaac tataattctt gccagccatg gggaatttgc
     1141 tcaaggatta aagcaaacag cgacaatgat tattggagat agtgtaccaa ttcatgcctt
     1201 gtcagcattt agagatgaag atgaatctat tttggtacaa ataaaaaaac ttttagccac
     1261 aatagatata gaagaaacct acattttaac tgatattcta ggtggtagtg tgaataatga
     1321 tatgctcact attattaaag agtatgaggg attgcaatta attactggaa tgaacttacc
     1381 tttagttatt tctattgcaa cctataatgg aaaaattggg gaaagtgagc tagaaaagct
     1441 tattaaagag agtcaacaat cagtgattga ttgtagaaaa ttattagtag aacaacttac
     1501 tggaggagat gacctatgat taagttagta cgtattgacc atcgtttgtt gcacggacag
     1561 gtagtatttt cttggagtaa atcattaaat atcaatcgta ttattattgc caataaagag
     1621 gcagcgaatg atgaattcaa aaaaatgtct ttgaatttat ctaaaccgac tggcattaaa
     1681 ttaacaattt tttcagttga acagttacta gaaaaaatgc ctaaaatcga aacacttaac
     1741 gaaaatatca tgcttatttt tggtaataca aaagaaattc gtcaattttg tgaagcctat
     1801 cctaaagtac aagaaatcaa ctatggaggg ataatcaaaa aagaaggttc caagcaattt
     1861 agtaatgcca ttttcttgaa tgaatctgag caagaagatg ccaaaaaatt aaaagctctt
     1921 ggcattaaac aatttattca acaggtacca acttctaaaa aagaagattt gaatacaatg
     1981 ttataaagga ggaatgaaaa tgctcgttca gtcaattggt ttagggttaa tcggtgtttt
     2041 ttgtatttta gactcaaggc ttttaggaag aatgaatttt gaacgaccgt tgattgtcag
     2101 tacgcttgtt ggtttggtac ttggagacat ggaaaaagga ttaattgttg gagcctcttt
     2161 ggaattgatg tccttaggat tagtaaatat cggagctgct gcaccacctg atatgaatat
     2221 ggcatcaatt attgctacag cctttgccat tttatcaaat gcgaatgcag aagcagcatt
     2281 aacgattgcc attccaattt cagtgttagg tcaaatacta ggtatcttaa tgcggacgat
     2341 tttgtcaaat ttgactcacg ttgcggatcg ctttatcgaa caagggaaat ttagccaagc
     2401 aaggagaatt catatacttt atggctcggg attatatgct ctcatgtatt ttattcctat
     2461 ttttctagca atttattttg gaacgaatct tgtttctcag attgttgatg cgattcctga
     2521 gtggctaaca aatggcttaa cggttgcaag taaaatttta ccagcatatg ggtttgcgtt
     2581 gttattacaa acgatgttaa cgaaaaaaat ggtgccattt ttattacttg gatttttaat
     2641 tactgcttat tctggattgg gaatcattgg tattgcagcc ttcgcatgta ttgttgcgtt
     2701 tgtcatggtt caagttcaag gtaaaaactc aaatggtaac caaaatgagc cagatccatt
     2761 agatgactta taaggaggta gattaagtga aagagaaaga tcaaaatgca tggaaatatc
     2821 ggcagttctt atggcgttca tgggcaattc aagcctcatg gaactatgaa cgacaaatga
     2881 acatgggttt tatgtacgga attgcaccaa ttctagataa catttacaag aatccagaag
     2941 atgaagcaat gaagaaagaa gcttacgagc ggcatatgat gtactacaac tgtacccctc
     3001 aaacaagtgc atttgtgtta gggttaactg cttctatgga ggagcaatac tacgaagacc
     3061 aagaaaactt taatccggaa tcaatcagtg cagtgaagac ttctctgatg ggaccgctat
     3121 ctggtgttgg tgattctttc tttcaaggaa cgattcgggt attagccttt ggactaggaa
     3181 ttaatttagc ccaacaaggt agtattttag gtccaattct agcaatgtta atttcctttg
     3241 taccttcctt tttagttact tactatggtg ggaaaattgg ctataacatg gggaataaat
     3301 acttgtctaa actgtatcaa gaaggtttga tggataaagt aatgtatgtt tgctccattg
     3361 tggggttgat ggtcattggt tcgatgattg ccagcatgat cgggattaca acaccaattc
     3421 aaatgggtaa agcttttgtc ttacaagatg tattagatag tattatgcca caatttttac
     3481 cattaggatt aaccttcttc atgtactggt tattgcagaa aaaggtcaaa acaggatgga
     3541 tgttggcaat ctgtttagtc ggaggaatta tttttagttt gttaggtatc ttacaataag
     3601 ggaataacca ataaatttgg ttgaataata taaataagga ggaattcaat tgatgaattc
     3661 aaaagaaatt gttaaagaaa tcgtagcgaa aaaagaggta aaatcaatcg tatttgttgg
     3721 ctgtggcgct tcaaaagcgg acctttatcc tgcaaagtat tttctagacc aaaatgcgaa
     3781 gcagttacgg attagtcatt acacagccag cgaatttaac tatgcaacac cagcatctgt
     3841 agatgagtca actgtcgtta tttcagcttc tttaggaggt acgactccag aaacagttga
     3901 agctaacgca gtagctaaag aaaaaggtgc tacagttatt agtctaacac gaagcattga
     3961 ctcaccgtta acaaaagatg cagattatgt aatttatcat cgatttgcag agaactatgc
     4021 tgctaaatta gaaaaaacag gctactgttt agaattggca gtagaacttt tacatcaagt
     4081 agaaggttat gaccattatg aagcaatgca agaaggattt gctactatct atgatcttgc
     4141 ccaaaaaagt gcagaatcag ctcgtaaaga tgcgaagaaa tttgctgaac gctttaaaga
     4201 tgaaaaagtt atttatgtaa tgagtagtgg tgctacacat gaagtagcat actcaacatc
     4261 aatttgcttg atgatggaaa tgcaatggat taattctggt accttccatt caggagaatt
     4321 tttccacgga ccattcgaaa ttgtggataa agacgtaccg tttatattat taatgaatga
     4381 tggaaaaact agaaatatag atgctcgagc attaactttc cttgaacgct ttgatacgaa
     4441 aacagaagtt gtagatgcct tggattatgg cttgtcttca catattgcta aagaagtctt
     4501 agactacttt aacccattcg tgattactgc agtatttaga gtttatgcag aagaattgtc
     4561 agaagcaaga gaacatccat taactaagag acgttatatg tggaaactaa cttattaaaa
     4621 aatagatcaa actaaataag gtggcttgag aaattaagcc accttgtttt cttcaatgtg
     4681 attttataca tatggtaaaa taactagtac atatggtaaa ataactagta catattgtga
     4741 aagcaggtgg caagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     4801 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagcttta cagttgaata
     4861 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     4921 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     4981 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     5041 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     5101 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     5161 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     5221 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     5281 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     5341 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     5401 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     5461 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     5521 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     5581 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagtaaaa
     5641 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     5701 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     5761 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     5821 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     5881 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     5941 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     6001 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     6061 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     6121 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     6181 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     6241 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     6301 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     6361 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     6421 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     6481 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     6541 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     6601 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     6661 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     6721 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     6781 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     6841 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     6901 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     6961 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     7021 agattccata tattggatta gatcgcctga ttaatgggga agcaattggc gaatttgttc
     7081 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     7141 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     7201 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     7261 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     7321 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     7381 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     7441 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     7501 gagtaatttt tgatatggat ggggtactca tagacagcga gacattttat tttgagagaa
     7561 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     7621 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     7681 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     7741 atttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     7801 cactggcatc ttcttctcca gttgttgaga tagagagtat gctcattcaa tgtgaactta
     7861 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     7921 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     7981 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggtggca ctaaaacaac
     8041 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     8101 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     8161 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     8221 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     8281 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacatt
     8341 ctatctattt cagaacgtaa ccttccccag ttcattggga ttcattagca tacaaccaac
     8401 atttcaaaaa gaaattgcac tacaccatta ttagctattt tgactaaccc caaaaaactt
     8461 gctataattc aggtgtaggt aaaaggtgct ggtatcacct tttacgaaac tgcttcggca
     8521 gtggtaatga gtgtggctta aaggactagt cctttaagct ttttactttg ttcgcaatct
     8581 ctaagatcaa gttaatgatc gataagattg ctagaatatt ttgcaacatt ctatttcaga
     8641 acgtaacctt tcccattcca ctgtgattca ttggcaaccc tcctcacaac caacgtttca
     8701 aaagaaattg taaagttgtt ggggtaccac gaccgaacaa tactacacct gaatgatagt
     8761 aagttgtctc attaagtata gcatatcctc tttttgggta aatagaaatc ggttacattt
     8821 tttatccaag accaagtttt ttcatttggt cttatttgct atacccaaac aattccgcag
     8881 tatagtcgac ggtattgatt ttatcaggtg agtaatagaa acttgttttc cttccccaaa
     8941 acattaccta taaaggagaa aaaatggtat cgtaattata taggtatagg atgaaatttt
     9001 tttctaagag gagggttgtt gatgaaaaat ttacagttaa aatttattgt tgatccatcg
     9061 attgtacgct ggtttcggat tatcaatcaa tttgagcgca atcaacttgc cacaaaagga
     9121 gaattagcaa agagtaatca tgtatctgaa cgaaccattc aaacagatgt caataaaatg
     9181 aaggactact tttctgaaag tgttcaattt gtttcaacga aaagtgggta ttcttttcag
     9241 aaggaaagac cggttctttt tctcaagcag aaggagcaac tactggagaa cgaaatttta
     9301 tttgagttgt tagggaatgt cttttatggc gaattggaag atatgacgga gctaattgat
     9361 cgttttcatt tatctgaagc gacttttcgt cggtatttga agcagattca accagttttg
     9421 gaagaatacg gcttagaatt gagtctttat cctcttgatt taaaaggaga agaggcaaat
     9481 attcgcaagt tttttaaaga cttctattat gaaggggaga tgacgcccca tactcttgtt
     9541 ccaccaaggg atcttcatga acttgtgcaa gctacattct atgataaatt tgagtatttc
     9601 tcgattggaa cagggacttc tcctgctgaa ttttattata gtttgtatat tgcgattgag
     9661 cgtgtgagac aaggaaaaac gatagccatt cctcaaggtc tgattgagcg agtaattgct
     9721 agtgagaatt ttcgcttaat gtattccttg aaagaagcga ttcaaacaaa ttatcaagtt
     9781 gctttatcag aagaggagtt ttgttggttg tat
//
LOCUS       DAPBBG010000081         9707 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPBBG010000081 DAPBBG010000000
VERSION     DAPBBG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.084"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(246..1088)
                     /locus_tag="RD477_002544"
     CDS             complement(246..1088)
                     /locus_tag="RD477_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7634856.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1125..2591)
                     /locus_tag="RD477_002545"
     CDS             complement(1125..2591)
                     /locus_tag="RD477_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7634857.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2647..4491)
                     /locus_tag="RD477_002546"
     CDS             complement(2647..4491)
                     /locus_tag="RD477_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7634858.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            5093..6043
                     /locus_tag="RD477_002547"
     CDS             5093..6043
                     /locus_tag="RD477_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7634859.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(6088..6721)
                     /locus_tag="RD477_002548"
                     /pseudo
     CDS             complement(6088..6721)
                     /locus_tag="RD477_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6981..7529)
                     /locus_tag="RD477_002549"
     CDS             complement(6981..7529)
                     /locus_tag="RD477_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634860.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7530..8387)
                     /locus_tag="RD477_002550"
     CDS             complement(7530..8387)
                     /locus_tag="RD477_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7634861.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8673..8960)
                     /locus_tag="RD477_002551"
     CDS             complement(8673..8960)
                     /locus_tag="RD477_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7634862.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(8950..9279)
                     /locus_tag="RD477_002552"
     CDS             complement(8950..9279)
                     /locus_tag="RD477_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7634863.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9356..9502)
                     /locus_tag="RD477_002553"
                     /pseudo
     CDS             complement(9356..9502)
                     /locus_tag="RD477_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(9554..>9707)
                     /locus_tag="RD477_002554"
     CDS             complement(9554..>9707)
                     /locus_tag="RD477_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7634864.1"
                     /translation="NKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIQA"
BASE COUNT         2927 a         1961 c         1491 g         3328 t
ORIGIN      
        1 gattcgtcat ggcaacgcat tgattttcaa ctaaaaaaga tcaagaggaa ctgatccact
       61 tgatctccaa ttattctaag atttctcttt accaacatca tttgacaaag agccgagatt
      121 ttttattatc ttacgttaat cctttttaaa tgtctcttat agataacaaa cagctggaaa
      181 tcaccttgtt tttactataa tccatctcta aagattttaa aagcatcaat agtattactg
      241 agcgctcacc cattagcatg tttcatcaac cgattgatgt ggacacctag gtatacaact
      301 tcatcgttcg gaattgtttt atgataaact ttttcaatgt agtctttgat cttagctgcg
      361 ctttccatgg cagacgggta taagatccac atttgattgt agaattcttc ctcaccatca
      421 tccaacaaag catcagaata atatctttct acaaagaaac gtacatgtgt aatgaagcgg
      481 gcataatgga tcgaattagt atcgatatct ttctgcaacg aataccggac caaattgacg
      541 atcccgccga tcatttttgc atacttgaat ccagcgccgc tatcaggatc ttccgattgt
      601 gcattgatca aatggaaagc aatgtttgag gcttcctctt taggaagatc aattccatat
      661 tgctcgttca aaagggtgag tgcgtacttc ccaatttgaa attctttggg atagtagatt
      721 tttatttccc agtacaaacg gtttgcaata tttacccctt tttgtgcgcg ttcaactgca
      781 aaatgcaggt ggtctgttaa ggttaaatag actccaggat tcagtttttt atctaatcct
      841 ttttctgctt caaaaacgat agtacgagtg atttcaaaaa attcaatagg tatttcttca
      901 actaactcta atagttgtga agatttcttt tcttcaaaag gcagaaatat tttatctact
      961 ttatctcctg atatttcatc cccagctttc tgtccaaagc ctatcccttt acctaatgca
     1021 atcatttctt tgccattttc ttcaacaagt accacacttg aattcaatac tttttttatt
     1081 gaaatcactc attatccccc tatcgctgct taaaaagatc gagactactc gaataaggat
     1141 gcaccgtttg tttcgatcac ttctttgtac cagttgaacg aatccttttt caagcggtta
     1201 tatgtcccat ttccttcatc gtctgcatcc acataaataa atccatatcg tttagacatt
     1261 tgagaagttc ctgcactaac taaatcaatt ggtccccagc tagtataagc tatcaaatct
     1321 acaccttcat cgattgcttc tttcatttgt tggaagtgtg ctttaaagta gtcgatgcgg
     1381 tacggatcat ggattttacc atctactacc tgatcgactg ctcccatacc attttccaca
     1441 atcatcaacg gtacttggta acgatcataa agttcgatca acgaaatttt caaacctttt
     1501 ggatcgattt gccagcccca atctgttgaa ggcaaatgag gattcttaac ccctaaaaca
     1561 gtatttcccg gtgtgcgttc tgcgtttgga tcagctgatt ctgccatgga catgtaatag
     1621 ctgaaagaaa caaaatctac ggtatgtttc tttaaaattt ctcgatcttc atcttccatt
     1681 ttaatctgaa ttccccgatt ttccaaatcc ttttcgatta gagcaggata ttctccctta
     1741 acttgaacat ctgcataaaa attattttct aagttctttt tcaaggtcag ctcaacgtct
     1801 tctggtgcgc aagttaatgg ataagtcatc aatttcgtta acatacaacc aacttggctt
     1861 cctggaattt tttcatgagc atcttttgtc actaacgcac tcgcaacaaa ttgatgatgc
     1921 aatgcttgat agatatcttg agcttcttga ccttctgcgc ttttctcttg gataattcca
     1981 gctgttgtaa atggatgacg gtggatgctg tctatctcat taaatgtcag ccaatatttt
     2041 acgtatttgc cgaagcgatc aaaacaaacg ttagcgaagc gaacaaaatc atcaattact
     2101 cgacgctcca cccagccatt atatttaact gctaaagcta atggcatttc ataatgagat
     2161 agtgtcacaa tcggctcaat tcctaaacgt tgcatttctt taaagacgtt ttcataaaat
     2221 gcaacccctt tttcattcgg ttccagctct tctcctgtag ggaacaagcg agtccaagca
     2281 atagagaggc gcaaggtttt aaagcccatt tcggcaaata aagctaaatc ttctttatag
     2341 tgatgataaa aatcaacgcc ccttcttttt gggtaccatt tatcagattt gtcttcacta
     2401 gcttctttta tagactgcag ggtgacatgc atatgagcag caaaatcttt gttgtctaaa
     2461 tttggtttat aagtagccat atccgctact gaaagacctt taccgtctac attccaagca
     2521 ccttctactt gatttgctgc tatcgcgccg ccccataaaa atccttttgg aaaacttgtt
     2581 gtgtttgtca ttattctatt ctcctctatt ccttcattca atatattttt tttcactatc
     2641 ttgtgtttaa gctatcggac tgatagctaa gagatcacta tcattggtta ccaatttttc
     2701 taggttaaat gaacggatag tgtaattatc cttattggtt accacacaga taactgtggg
     2761 gtcgaatcct gcttctttga ttaaatcaat atcaaatgca atcagctttt gtcctttggc
     2821 tactttttca ccagaacgaa caaatgcctc aaaccctttt ccttctaact gaacagtatc
     2881 aataccaata tgaaaaagaa gttctgcacc attagccgtt tttaaaccaa tagcatgctt
     2941 cgtttcaaat accattgtga tttctccatc ttcaggagct actaatatcc cttcttcagg
     3001 aatgattgct actccatcac ctaccatacc actagaaaaa acagtgtctt ttacttctga
     3061 tagtgagatt acttttccat tcacgggact tgaaagaaca gcatctgaaa caacggccgt
     3121 ttccatctta gtttcaactt cattgacaac ttctttatct ttataaagaa ccaacgctgc
     3181 agcaaaagca accacaaatg aaatccccga tactgcaaat gcccaaacca gattcattgg
     3241 gtcagctttg tcagcgaaca tcgtgatact tgcaatccca ggacccacta aagcaaaggc
     3301 ttgaagtgca acgattcctg caaatgctcc cccgatcagg ctagaaaaca taacggcata
     3361 taaaacacgt ttgtgcagaa tcgttacccc gtaaagagct ggttcagtaa ttccgaataa
     3421 tgcagatata cccgcagaaa cagccgtaga tttcaatttt ttgtcttttg ttttgatact
     3481 gacagcaaag catgcgccag cttctgaaat attgtgtgcc aatgaagccg ggagataaag
     3541 aagttctgca cccaattcac tcatggatga aacagcgtaa gggatcattg ctttatgcat
     3601 acccgttaca accattaatg gcagtacagc tgctaagact ccggttgcaa cccatccaaa
     3661 ttgattgtac aaagacacaa tgatagaaga gaaagctgtt cctacattat aacctaatgg
     3721 tcctagaagc aacaaagcaa taggaactgt aatcaataag ctcatcatag gtacaaagaa
     3781 aatacgaatt ggttttggtg aaacttttgt aaaccatttt tcaagttgag cataaaataa
     3841 aacagttaag atggctggga atacttgaga tgcatacgct atattatcca atccaaaaga
     3901 taagaatgta gcaccttccg ccaacatagc tgtcatttca ggaaggatca atgcaccgac
     3961 agcagatact gcaaccaatt gattgacttt caatttatta gccgtagtca ttgccaccag
     4021 aataggcaag aaataaagcg gtgccccgcc gaccaaattt aaaatctgat aggtttgaga
     4081 ttggtcacta atcagcccta agatagaagc tagtagcaac aaggacttca atactcctcc
     4141 acctgcaatc gcaggaatta aaggttggaa aatagaaata ataaaatcta atagaactga
     4201 acctaatttt tgtttttgtt ttggtgcttc ttcagtattt gaaacattgt cacctaataa
     4261 cgctttcact tcatcataca cttcaacaac atcgtttcca atgattactt ggcattgcac
     4321 attttggcga acgcccataa cgccatccac tttttctaac tggctcatat ttaccttgct
     4381 gttgtctttt aacgtaaaac gtaaacgagt tgagcaatgc tccaaatggg atatgttttc
     4441 ttccccaccg atgtactgca atacttcttc tgctgtttgt ttgtaattca tggtgtttcc
     4501 ctcttttctt ttttgagtga tgaacttttg agtcgaagtg gccatcctca acacaaaaaa
     4561 caggcagaaa ctaaataaat tgacaacaag gcaaacgtgt attgaaacgc atacattgtt
     4621 attcaactta tttaggtcct gcctgcatta ccagtcacat gcctgtttaa gttatattca
     4681 atttaacata ttagaatgta aacgtaaata ataaaatgat tttttaatca ttttttcatt
     4741 ttattcgttc aatatagttt tcgtgttcta aaagttttgt cttttttcta agaatatctt
     4801 ccaacatata taactcttat tctcttctat cttctagcag atgttctgtc tttttaagac
     4861 ctagtataaa aagaatgggt tcattttttc tttcttgcag gatgaggttt tttatataaa
     4921 ctttatttga tataaagaaa aggcattcat aattgtgcta tactactaaa aaatgataat
     4981 acagtgaatt agattagcgc tttattaaat taagaggaca gcatatcgat attgcttcct
     5041 tttttttgct atttatttaa gggatagaag tttagaaagg actgtttata agatgaaaaa
     5101 tgatatagtg acttatgcaa acacaaaatg tgaagaaatt ggcatcagta atttagaaga
     5161 gtgctttttt gacacaaatg acctgtcgca atttttaaga acgggaagaa caaatgccag
     5221 taaacttgcc aatgacttag ttgcaaagaa cgttctattt aaaatcaata ctcgtccagt
     5281 gttatttgcc atcaaagagc gctttgaaga gcactttttg attccggtaa aagatgtata
     5341 cgaaagtata tctgaattaa aaacagactt ggagcccaat tctacagatt ccattctaca
     5401 aaagatgatt gggtatgata tgagcttatc cgatgcggtg gaacaaatga aaactgctgt
     5461 cttctatcct ggaagagggc ttcctatgat gatcactgga gatactggag ttggaaaaag
     5521 tttttttgtt catactgctt atcagtttat gaaaaaagtg aacgttattc atgagaatgc
     5581 tccttttaaa gtacttaatt gtgcgcaata ccataataat cctgaattat tgtcggggat
     5641 actatttggc tatgttaaag gtgcttttac cggcgcagat gaagatcgaa atggattgtt
     5701 gcaagatgca gacggtggag tactgttttt agacgaagta cacagattgc ctcctgaagg
     5761 gcaagagaaa ctatttgttt atatggatgg cggaacttat tctagagtcg gtgaaactat
     5821 ccatcacgcc tccaatgtac gtttagtttt tgcgactaca gaaggcaaag aggattttct
     5881 ggaaaccttt atgcgaagaa tccctataca tatccatatc ccaaatattg aggaacgagg
     5941 tattttagaa aaaaaacaaa ttatcgaaaa tactctcttt agcgaaagca aaaactttag
     6001 caaggctatt gatgtttctc ctaaagtcac tagtgttgca taaatatagt cagaatattc
     6061 tgaattatta ttttcttgaa aaaagtaaaa aaaatcgtat cccaaacttt catctacatt
     6121 tccagaaaga tcttcatgtt gataatcaat tcctgaatca atgaccgcta ccttcacatc
     6181 cttagaaccc ttggtttctt cccaagcgga tggcatgcta atctctctta aattagcttg
     6241 tttactccaa ttaggatcgt tatcgatttc ctaaagaatc agttgtattt ggctcctcaa
     6301 catcattcaa cggctctagt tcaaccacat agtttggctc tgcataattg accatatcag
     6361 aagcattaaa tgttcttact gcctcttcca cactaatttc ttcgttcaat ttcactaata
     6421 atacttgatc tcgcttatta taatcgcttt catttgtcct aagagtcaaa tctttttcca
     6481 ccataacttt tacacccaat aaattttgcg gtttattttt ctctgttgaa attctttgtg
     6541 tagcctttaa actgacaatt agctcattgg gtacatactc tatttgagag tcatctaatt
     6601 ggctcataat cctttctttt tggttagtat ctgcagttac ttgtttagta tgagccatga
     6661 acccaaacat actcacccca acaaccaatc ctaaatatat tttcttttta aactttttca
     6721 tctacatctc ctcctaatag ttttatttta gagttagctc tattacttat ttactaactc
     6781 aacaacagat tatcatacac ttattaatga aatcacaata tctcattaaa acacacccta
     6841 aaatcataac cgttcctatg ctcatttttt ggatttttta atcacgtaac cagaaaaata
     6901 aatcccctct atttcacgca aatcaaagaa gaactaagta actaccgttt aaaggtagtt
     6961 acttagttct tctttgatta ttatctaact ttattattca ttccccaccc tttgcgcagt
     7021 gagtcttcgg tatcttgttt tatctgactg gtagttctta ttatttctcc acgatcatct
     7081 aattctggaa caattggata tttttctctg aacaagtcta gttctttcac cacgtcatta
     7141 aacaaatctt gtttatcttc ttgcgtaaga ttctgtttaa tatactcatc taaagctttt
     7201 ctaagcaatt catctcttgt catattagtc tcatactttt ctactaaaac tctggaagtc
     7261 gcatttaaca aagtcgacag ttttaaagga tgcttggtgg aataaacctt atcaaacttt
     7321 ctgattggtg cgccaacttt ttttgttctt cctctactct gtctttcaac attggaggaa
     7381 gtttttattt cttgaacttg gtgcacttct aattgcttat tttcagatgg tgtaattgct
     7441 tgctgctttt tctttgcttc tgcttctttt tttattttgg cgagtccttt gttgttccca
     7501 aaaatatcaa gtcctttgat gtcagccaat tactccacct ccgcatacca attctcgtgt
     7561 tcttccaatt ctttcaaaat actgataaat aattcgtggc ttttgcgatc ccattgtttt
     7621 aagagacctt tctttgtctt ctccatcgta atacctgatc cgtcgtatcg ttttaagcgc
     7681 gcatttttga gtacaacatt tttaatcaaa tgttccccat aaatctcttg ggcttgttta
     7741 tacatttcct gatctactgc gtcaccgctt tccaacatga aaggaataac accagcaaca
     7801 tataaatttg tatcaaattc tttcacaaaa aagtccatat actcaagata ttgcatcgca
     7861 tttctcatcg attttacttg ggtctgtaag acgacgattg cccaatccgc aatgtacatc
     7921 gcggatttac tatatacaga aatagaaggt gggacatcta aatagatgac gtcaaactca
     7981 tcaaataatg gttctagctt ttctttcata tatttgaatt gggataatga atcattatat
     8041 tggtctttga tccaatcatc atacatttca aaggacatgt ctgctggaag tatatagagg
     8101 ttattgatta ctttatagac agcctcttta ggtggaagat tgtttatcaa cacgtcagtc
     8161 acagtatttt cgtaaacaat cggttcacct tctccgcctc cttcttcgtt agtgaagttc
     8221 atatgcgtta agttcatcac atcagaagta tttccttgca tatccaagtc aattatcaag
     8281 gttctttttc cttcattaac tgctgattca aaccctaaca tttgcgcgca cgtactttta
     8341 cctacgccac ctttaaaatt accgattaca tatttcttcg ctaccacgtt cctacctctt
     8401 ttcttttagc aatattcatt tgatactatt ataacatatt tatttttagt tactaccaaa
     8461 aaaaagatat atatgtacta ccttataatt aataagtaaa aggtagtaca taaataagga
     8521 aaagctgaaa tatattgatt tatagctttc ctcaaagtaa tgacgttatt taatttatcg
     8581 agaagcagag aatactcgcg actttagtca tgtagacgaa tcagattttc gataaaaaag
     8641 aagccgtcct tcttaagaac gacaacaaat cactagatta attttttcaa ctgttcataa
     8701 aagttttcat gcgttccaca taacaatact ggaataacct tgttttctat aatctcgtaa
     8761 gctactctat atgttgtgcc cgcatatttg aacccactag accaaatacc tgataaatcc
     8821 ccagtttttt gttcaccctt atatggatca atcgcaattt catcataaat taaattcaaa
     8881 aatttttctt tcaatggctt ctcttttagc ttttttaaat acttaatgac atgttttgaa
     8941 ggaacgattt cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt
     9001 agtaagtgta tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt
     9061 aaattctaca agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc
     9121 ttcgctaaaa tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc
     9181 taccaacttt gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa
     9241 catgattgaa ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca
     9301 gtatgtaatt acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact
     9361 gtgactcgta atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct
     9421 tataaacaac ccggtgttgt ctattcaatc ttctaaaata cctaccttca tgtgtaggtt
     9481 ttaacttctc aaatgattgg gttctgttgc aaagttttaa ataaagaata aaatccctta
     9541 cggtatctat gatttaagct tggattccca ataatacctt gatttcagta cagaccgaaa
     9601 acccgaagag agtgccttct tttcgggttt tcttatataa tcctcgaatg gcttccatgc
     9661 ctttaatcgt ggtagaggca gtgcgtaaac ttcgatagaa tttattg
//
LOCUS       DAPBBG010000082         9321 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPBBG010000082 DAPBBG010000000
VERSION     DAPBBG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.085"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            453..1025
                     /locus_tag="RD477_002555"
     CDS             453..1025
                     /locus_tag="RD477_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDT7634865.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1170..1874
                     /locus_tag="RD477_002556"
     CDS             1170..1874
                     /locus_tag="RD477_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7634866.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            2102..2440
                     /locus_tag="RD477_002557"
     CDS             2102..2440
                     /locus_tag="RD477_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634867.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2425..3279
                     /locus_tag="RD477_002558"
     CDS             2425..3279
                     /locus_tag="RD477_002558"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDT7634868.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="RD477_002559"
     CDS             3426..3659
                     /locus_tag="RD477_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDT7634869.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3857..5761
                     /locus_tag="RD477_002560"
     CDS             3857..5761
                     /locus_tag="RD477_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDT7634870.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            6115..6480
                     /locus_tag="RD477_002561"
     CDS             6115..6480
                     /locus_tag="RD477_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7634871.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            6497..7108
                     /locus_tag="RD477_002562"
     CDS             6497..7108
                     /locus_tag="RD477_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HDT7634872.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            7136..8797
                     /locus_tag="RD477_002563"
     CDS             7136..8797
                     /locus_tag="RD477_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDT7634873.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            8825..>9321
                     /locus_tag="RD477_002564"
     CDS             8825..>9321
                     /locus_tag="RD477_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7634874.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     MTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIGADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKASSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNL"
BASE COUNT         3024 a         1505 c         2029 g         2763 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tagaatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaag
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtgaaa tcagttaacg gtattgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa accaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg ttcttaaagc aaaagtcaag gccgtttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacgttct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaattgac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 agtggatgat atagatgaag gggatatctt gctcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggtgaagc
     4381 tattccggtc aacaagctag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggaa aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcggaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagtaatgt ggatacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg agaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa ggaactgaag ttgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc tcagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggctacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcagttg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcgg aaagaacaaa aagaagtatc agcacctaca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa taagagtata
     5881 gagtatgttc tttataaaat taattttcgt gtaaattatt aaaaatttaa taataaaatt
     5941 taaattccta ttaagttgta acacatgaaa ttgcttaaaa tagtgtatca aaaaccttgt
     6001 tttattaatg ttgaatttat ttgaaaggtc tacagattaa catattattg acaaaacaat
     6061 atttaaatag ttatacttca ttcaaacgtc tgtttgaacg aggtggtgaa gttaatgact
     6121 gcagaagtat gtacaactac ttgtatacat aaagaaaaag tagctagtgt taaaaataat
     6181 ttaaaagata aagacctttc aactttactt gtattaggaa aatgttttag cgattcttct
     6241 aggattaaaa ttttctatgc tttagaaacg tataaggaga tgtgcgtttg tgatttagca
     6301 gcgatactta atgctagtat tgctacaacg tcacaccatt tacgtttttt gaaaaagaac
     6361 ggaatggcga aatcacgtca atatggaaag gtagtttatt actctttagc gaatgaagag
     6421 attctttccg ctgtgcaggc ttttctaaag ttgtcagaaa atagttctat gaaagcttag
     6481 tttggaggta gttattttgt tacaaaacat tttatcagca ctcgctgtat atatttctac
     6541 cagtattgat tatttattta tcttgctgat tattttttct caaaaccata ctaaaaaagg
     6601 tctccgtcag attttttttg gtcaatatct tggaacagga atcttggtag ccataagttt
     6661 gtttgcagca tatgtgctaa attttattcc tcaagattgg atcattggtc ttctgggact
     6721 aattccgatt tatttaggga ttcgtgttgc ttttgtgggc gaagaggagg aagaagaagg
     6781 ggaagtcgtt gagaagctag gatctagagg aacgaatcgt ttgttttgga cagtttcctt
     6841 aataacgatt gcttctggtg gcgataattt agggatttat attcctttct ttacctcgtt
     6901 atctttttca gaaattgtca cttctttaat cgtctttgct atctctgttg cggttctttg
     6961 ctatattagt tataaatttg cgaaaatttc ttttgtctct gaaacactag aaaaatatga
     7021 acgaatcatt gtacctatcg tatttattgg attagggatt tttattatga tagaaaatgg
     7081 aaccatccaa actatattag gtttgtagag atcttattag taaaggaagc aaacgatgag
     7141 tgaaaataaa aagtaccatg aatggatatt agaaggaatc gactgtgcaa attgtgcaga
     7201 caaagtagaa agaggcgtcg ccaaagttgc aggtgttgcc aatagtagtg tgaactatat
     7261 gacagaaacc ttgagtttcg aagtgacaga ggaaaaagaa tatcttgttt tatccaatgt
     7321 gcaacaaaaa gtaaaaaaat tggagcctga tatcacttta aaaaataaag tggacggtag
     7381 gctgattgaa ataaatccgg cattaatcaa gaaaagtaat ttaaaagacg ataaaaaaaa
     7441 ctacgaatgg atattagaag gaatcgattg tgcaaactgt gctgccaaag tggaaagagg
     7501 agttgctaca gttcccggcg ttacaaacag cagcgtcaat tttatgacag agacactgag
     7561 cttcgaggta gcaggtgaaa ataaaactga cgtgttggct agtgtaaaac aaacagtagg
     7621 aactttagaa cctgacgttg ttctgcgaaa aaaatcggac ggtacattga ttgaacatgg
     7681 aactgactct aataaaaatt cgacttctaa caaatcgatt tctgataaag atgagacaaa
     7741 ccaaaagcag acgaatagag cttatctggc cagccttctc ggaaataaaa ctaaaattac
     7801 ccttattcgt ttaatactag gattaggaat attagcgaca ggaatatttc tccctatgga
     7861 tggacaagcc tctcttgggt tgttcattct ggcttattta atcaccggtt atgacgttgt
     7921 aggacgtgcc attagaaata ttttccgagg tcaagtattc gacgagaact ttctaatgac
     7981 cattgcgacg ttaagtgcct tctatattca agagtatccc gaagctgtag cagtcatgct
     8041 attttatcaa gtgggagaag tattccaaga tattgctatt tcaaaatctc ggcgctccat
     8101 tgcggaccta atggatattc gacctgatta tgccaattta gtcacaggaa acaacgtaga
     8161 aaaagttgct cctgaagcaa ttaagattgg aaatattatt ctcattcgtc cgggcgaaaa
     8221 agtgccattg gatggaaaag tagtggaagg tgcttctgcg atggacactt ctgctttaac
     8281 aggcgaatcc gttccaagaa gcgtgaagcc ggaagacact attttgagcg gatttattaa
     8341 taaaaacgga ttgctaaaag tagttgtaga aaagcccttt tcggagtcta ctgtgaagaa
     8401 aattttagat ttggtccaaa atgctagtgg ccggaaagca cctactgaac aattcattac
     8461 caaattttct cgctactata ctccaattgt ggttatagct gctgccttac tggcaattgt
     8521 tccgccattg ctagtaccag gcgctacttt taatgaatgg atatataggg ccagtatctt
     8581 tttagttatt tcatgccctt gtgctttagt cgtttcgatt ccagttggtt tctttggtgg
     8641 gattggttct gcttcccgca aggggatttt agtaaaagaa agcaatttcc tcgagggttt
     8701 gaacgatctt aaatacgtgg taatggacaa aacgggtaca ttgacaaaag ggttctgttg
     8761 caaagtttta aatctactat caaataaggt agaataatag aaaaagatag taggaggaat
     8821 gacgatgaat cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg
     8881 atactatctt cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg
     8941 cattaacgtt tctcatatga cgatttatcg ttgggtgcaa gaatatggca aactactcta
     9001 tcaaatttgg aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta
     9061 catcaaaatt aaaggaaaat ggcattattt gtatcgagcc atcggtgcag atggtttaac
     9121 cttggatatt tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg
     9181 gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca gataaagcct cctctattac
     9241 aagtgccttt aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat
     9301 taaatacctg aataatttga t
//
LOCUS       DAPBBG010000083         8758 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPBBG010000083 DAPBBG010000000
VERSION     DAPBBG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.086"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     regulatory      408..522
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            658..1758
                     /gene="ribD"
                     /locus_tag="RD477_002565"
     CDS             658..1758
                     /gene="ribD"
                     /locus_tag="RD477_002565"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDT7634875.1"
                     /translation="MSDIEYMKIAIELAKKGAGYVNPNPMVGAVIVKNNRLIGQGYHE
                     VFGGLHAERNALKNCRESPVGATLYVTLEPCCHYGKTPPCTETIIKSGITRVVVGTLD
                     CNPIVSGKGVKQLEENNIQVDVGILEIECQQLIKVFRKYITKRIPYVFMKYAMTMDGK
                     IATYTNQSKWISGEKARKQVHQFRHKVTAIMVGVNTVIQDNPLLTCRLENGKNPIRIV
                     CDTDLRTPITSKIIQTANDIKTYIATSSIDESKIALYRKCGCEIIYTKKKGNHIDLMN
                     LMQCLGNMQIDSLLLEGGSALNWSALEQQIVDEVQTYIAPKIFGGSGKSPVSGQGVVF
                     PKDAIMLKPYAFSQVGNDYLIESEVIYPCLQE"
     gene            1743..2378
                     /gene="ribE"
                     /locus_tag="RD477_002566"
     CDS             1743..2378
                     /gene="ribE"
                     /locus_tag="RD477_002566"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDT7634876.1"
                     /translation="MFTGIIEEIGKVERIQKDTYNCKLSIKSSKILEDIHLGDSIAVN
                     GICLTVTCFTRQSFIVDVMNETWNRTALNLLKHGSLVNLERAMHMNGRLGGHIVTGHI
                     DGTGRISSVRRDKNAVWYQINTQREILNLIVEKGSVTIDGISLTVAKVSKADFSVSVI
                     PHTLEQTILKNKQVGDMVNLENDILGKYVKKLIGNSNETELSKELLCRYGF"
     gene            2398..3603
                     /locus_tag="RD477_002567"
     CDS             2398..3603
                     /locus_tag="RD477_002567"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDT7634877.1"
                     /translation="MKYKKIKEALQALQEGKLVLVMDDKERENEGDLICSAQFATTEN
                     VNFMATYAKGLICMPMSESLANRLMLSPMVENNTDNHETAFTVSIDYKDTTTGISAEE
                     RGLTARMCVVEDINPFDFRRPGHMFPLIAKNGGVLERNGHTEATIDLLRLAGLKECGL
                     CCEIMNENGKMMRTSDLIQFSQIHHIPTLTIKELQEYRKVYDMLVECVSVVEMPTKYG
                     DFKAHCYINKLNGEHHVALVMGELNSGNDVLCRVHSECLTGDVFGSLRCDCGQQLDRA
                     MKMIAENGSGVLLYMRQEGRGIGLVNKLKAYHLQDKGMDTLDANLALGFQGDLREYYI
                     GAQILRDLGIKSLHLLTNNPDKVYQLEDYGMQISSRVPIEIKANPYDSFYLKTKKDRM
                     GHILNMEEK"
     gene            3604..4071
                     /locus_tag="RD477_002568"
     CDS             3604..4071
                     /locus_tag="RD477_002568"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDT7634878.1"
                     /translation="MNTFEGNLVAENIKIGIVVARFNEFITSKLLAGALDNLKRENVD
                     EKDIEVAWVPGAFEIPMIASKMVKSKKYDAIICLGAVIRGSTSHYDYVCSEVSKGIAQ
                     ISLNTETPVMFGVLTTDTIEQAIERAGSKAGNKGSECAQGAIEMVNLIRALEV"
     gene            4690..5904
                     /locus_tag="RD477_002569"
     CDS             4690..5904
                     /locus_tag="RD477_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDT7634879.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRIAVACGISREYYNRIEKGKQP
                     LNNELKEIIEKQIERFNPREPLFLLIDYFRVRFPTTDALKIIRDVLQLKADYMLYEDF
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLGCMT
                     AGGKLKRLDLAINDKAGILDIPKLIEKYKAGECISYFRKQKDYGGTEKNGYDTPQNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKMNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDKENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTVVPQENDGIF"
     gene            6623..6833
                     /locus_tag="RD477_002570"
                     /pseudo
     CDS             6623..6833
                     /locus_tag="RD477_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            6945..8558
                     /locus_tag="RD477_002571"
     CDS             6945..8558
                     /locus_tag="RD477_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7634880.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEVGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKILREEKVPTSSWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAQIKGILKSEVYIGNSVHNRQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIEKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYACSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKASALKKAENRQNEIDCLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITDLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            8656..>8758
                     /locus_tag="RD477_002572"
     CDS             8656..>8758
                     /locus_tag="RD477_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDT7634881.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAII"
BASE COUNT         3155 a         1281 c         1893 g         2429 t
ORIGIN      
        1 aatattacta actttaatgc agtataaaga gtatccagag tattttattt taacttttgt
       61 atatttagta agtagctaca cacttctata taaattcaga agttcttcta gagtttctaa
      121 agtttgtttc ttaattagct tattgattct ataatatttt tgatttatat aggaaattaa
      181 aaatattata gaatcaacac tcagagaacg ggttggtttt taatagattt aataagtact
      241 gataatgaaa tttctcctgg ttgggaaaac gaagtttccc aaacaaaagg atattaagaa
      301 ataacctgtg tttacctgct tgcaaattat tgtattttgg ataagcaaga gaaaaacttt
      361 ttattgacag aaataatatc aaatattata attgtccgta attgaataac tatcttcagg
      421 gcagggtgta attccctacc ggtggtacag cccacgagcc gaaaggcatg atttggtgta
      481 attccaaagc cgacagtata gtctggatga aagaagataa aagtaagttt atgctttttt
      541 gcgtgctctg gaatgatttt atattgtttc agagcatttt ctttaatcgc ataaatcctc
      601 ttttgcatgt cttggataga ttttattcaa ggcatgtatt tttttggagg tgtaaaaatg
      661 agcgatatag aatacatgaa aatagcaata gaactggcaa aaaagggtgc tggctatgtc
      721 aatcctaatc ctatggttgg agcagtaatt gtaaaaaata atcggcttat aggacaggga
      781 taccatgaag tttttggtgg attacatgca gagagaaatg cactaaaaaa ttgcagagag
      841 tcacctgtag gagcaacgct ttatgtaaca cttgaaccat gctgtcacta tggtaagaca
      901 ccaccttgca cagaaacaat tattaaaagt ggtattacaa gggtagtcgt cggaacttta
      961 gactgtaatc cgattgtgtc tggaaaaggt gtaaagcaac ttgaggaaaa taacattcaa
     1021 gttgatgtag gcattttaga aatagaatgt caacagttaa tcaaagtatt taggaagtat
     1081 attacaaagc gtattccgta tgtttttatg aaatatgcaa tgacaatgga cgggaaaata
     1141 gcaacttata caaatcaatc aaaatggata agtggcgaaa aagcgagaaa acaagtgcac
     1201 caattcagac ataaggttac tgcaatcatg gtaggagtaa ataccgttat tcaagataac
     1261 cctttactga catgccgatt agaaaacggg aaaaatccta ttcgtatcgt atgtgataca
     1321 gatttaagaa cacccattac atcaaaaatt atccaaacag cgaatgacat taaaacatat
     1381 atcgctactt cttctattga tgaaagtaaa attgctcttt acagaaaatg tggttgtgaa
     1441 attatttata cgaagaaaaa aggtaatcat attgacttaa tgaatttaat gcaatgttta
     1501 ggaaatatgc agatagacag tctgctttta gagggtggaa gtgcgttgaa ttggagtgct
     1561 cttgagcaac agattgttga tgaggtacaa acttatatag cacctaaaat atttgggggt
     1621 agtggaaaaa gccctgtaag tggtcaaggt gttgtttttc ctaaagacgc cattatgctc
     1681 aaaccatatg ctttttctca agtgggaaac gattatctca tagaaagtga agtgatttat
     1741 ccatgtttac aggaataata gaagaaatcg gtaaagtaga aagaatacag aaagatactt
     1801 ataactgtaa actatccatt aaatcttcaa aaatattaga ggatatacat ttaggcgaca
     1861 gcatagcagt caatggtatc tgccttacag ttacttgttt cacacgacaa tcttttatag
     1921 ttgatgtgat gaatgaaaca tggaacagaa cggctcttaa cctgttaaaa catggaagcc
     1981 ttgtgaattt agaaagagcc atgcatatga atggcagatt gggcggtcac attgttacgg
     2041 ggcatattga cgggacaggc agaatatcgt ctgtgcgtag agacaaaaat gcggtgtggt
     2101 atcaaatcaa tacgcaaaga gaaatcttaa atctaattgt tgaaaaaggt tcggttacta
     2161 ttgatggtat tagtcttact gttgcaaaag tatcgaaagc agatttttca gtatccgtta
     2221 tccctcatac tttggaacaa acaattctca agaataaaca agtcggggat atggtaaacc
     2281 ttgaaaatga tatattaggg aaatatgtga agaaactcat aggaaacagc aatgaaacag
     2341 agttgtcgaa agagttgtta tgccgatacg gattttagca gaaaggataa aggaattatg
     2401 aaatataaaa aaataaaaga ggctttgcag gcgttacaag agggaaaact tgttttagtt
     2461 atggacgata aagagagaga aaatgagggg gatttaattt gctcggcaca atttgctaca
     2521 acagaaaatg taaattttat ggcgacttat gcaaaaggtt taatctgtat gcctatgagt
     2581 gaaagtctgg ctaacaggct tatgctttcc cctatggttg aaaataatac agataaccat
     2641 gaaactgctt ttacagtttc tattgattat aaggatacca caacaggtat ttctgccgag
     2701 gaaagaggat tgacagctcg tatgtgtgtg gttgaggata taaatccctt tgatttccgt
     2761 agaccggggc acatgtttcc tctgattgca aagaacggag gtgttttaga aagaaacgga
     2821 catacggaag caacgattga cttattgagg ttggcaggat taaaagaatg tggcttatgt
     2881 tgcgaaatta tgaatgaaaa cgggaaaatg atgagaacat ctgatttaat tcaattttcc
     2941 caaatacacc atataccaac cctcacaatt aaagaattac aggaatacag aaaagtatat
     3001 gacatgcttg tagaatgtgt ttcagttgta gaaatgccta caaaatacgg ggatttcaaa
     3061 gcacattgtt atatcaataa attgaatggc gaacatcatg ttgcattggt tatgggagag
     3121 ttaaatagtg gaaatgatgt gctatgtcgt gtccactcag agtgtttaac aggagatgtc
     3181 tttggctctt tacgctgtga ttgtggacag cagttagata gagcaatgaa aatgattgca
     3241 gaaaatggtt ctggcgtatt gctttatatg cgacaagagg gacgaggtat cgggcttgtc
     3301 aataaactaa aagcctatca tttacaggat aagggtatgg acacacttga cgccaacctt
     3361 gcactaggat ttcaaggcga tttaagagaa tattatattg gagcacagat tttaagagat
     3421 ttgggaataa aatcattgca tttacttaca aataatcctg ataaagttta tcagttagaa
     3481 gattatggaa tgcaaatttc aagcagagta ccgattgaaa taaaagcaaa tccttatgat
     3541 agtttttatt taaagacaaa gaaagaccga atgggacaca ttttgaatat ggaggaaaaa
     3601 tagatgaaca cttttgaagg aaatttagta gcagaaaata taaaaattgg tattgttgta
     3661 gcaaggttta atgaatttat aacttctaag ctattagcag gtgcattaga taatttgaaa
     3721 agagaaaatg ttgatgaaaa ggacatagag gtagcttggg ttccgggagc atttgaaata
     3781 cctatgatag cgtctaaaat ggtaaaaagt aaaaaatatg atgcaattat ctgcttggga
     3841 gcagttattc gaggtagtac aagccattat gattatgtgt gtagtgaggt atcaaaagga
     3901 attgctcaaa tcagtttaaa tactgaaaca ccagttatgt ttggtgttct tacgacagat
     3961 acgattgaac aggcgataga acgagctggc agtaaggcag gaaataaagg ctctgaatgt
     4021 gcacagggag ctattgaaat ggtaaatcta attcgtgcat tagaggtata atgttgatca
     4081 gcacattata atatgccatt ttcagagaaa taaatagtgc agaaataata cggggatctt
     4141 ccatccccga acccctggaa tccttgttat aacagtaata gaaaaaatta ttaccgttat
     4201 aacaaggcta aaaatgggat gttttaattt tccgaattct gcaaattatg gcatcaaata
     4261 aaatgcgttg taacagtata atatgcttgg ggttaaaagc taaagcgaaa aaacataaca
     4321 ggcggagtga tccagacacg acggtgggaa aatctcttag atttttccgc cgtttttagt
     4381 ggctggttca cttcggcagt tgagcgagct tgcgagcgaa cagtaagccc ccgttatcta
     4441 acaagggggg cacaaaaaac aagagacaat agacactact aagcggaaaa cgctgtattt
     4501 acaaggcaaa accgctttta acagggtgtt aactaaaaga cactcattta acacataaaa
     4561 ttgaataaga cataggaaag acagttgatt tcaaatgaaa aattgattgt ctttttcttt
     4621 tgaagaaatt taagtataga caattatcag taaatggtag ttgtcttttt tgattggagg
     4681 aattgaagaa tgaatgataa gaactttata gaagaattaa gacaaaagcg tgaagaatac
     4741 ggagtaacac aaacaagaat tgctgttgcc tgtggtatca gtcgtgagta ttataaccgc
     4801 attgaaaaag gcaaacagcc attgaataac gaattaaagg aaatcataga aaaacagatt
     4861 gagcgtttca atccacgaga gccgttattt ttactaattg attactttcg tgtccgcttt
     4921 cctactacgg acgcattaaa gattattcgt gatgtattac aactaaaagc cgattatatg
     4981 ctttatgaag attttggaaa gtacggatat gaaagtaaat atgttttagg cgacattaac
     5041 atcatgtgtt ccatgcaaga gcatttaggt gttttgttgg aactgaaagg cagagggtgt
     5101 cggcaaatgg aaagttatct cttggcacag gaacgctcat ggtatgactt tatgcttggt
     5161 tgtatgacgg caggcggtaa gctgaaacgg cttgacttgg caatcaatga taaggcaggt
     5221 atcttggata ttccaaaact aatagaaaaa tataaggctg gagaatgtat ctcctatttt
     5281 cgcaaacaga aagattacgg tggtacagag aaaaatgggt atgatacacc acaaaataca
     5341 ggcgaaacct tatatctcgg ttcaacaagt agcgaattat atatgtgtgc ttatcagaag
     5401 aattatgagc agtatgtcaa gaatggcaca gaagttgaag atacagaaat caagaaccgt
     5461 tttgaaatac gcatgaagaa tgaacgagcc tattatgcgg ttgtagattt actgacttat
     5521 cgggacgctg aacgtactgc tttttctatc atcaatcatt atgtccgttt tgtagataga
     5581 gaggacgata aaccgaaaag tcaatggaaa atgaatgatg attgggcgtg gtttgtagga
     5641 gaaaacagag agccgatacg cttaaccaca aagccagagc cttatacttt acaaaaagca
     5701 ttgcattggc tacaaagaca ggttgcacca accataaaaa tggtacaggc attagacaaa
     5761 gaaaatcata caacgatact aaaagatatg attgaacagg cagaactcaa agacaagcat
     5821 aaacatttat tacagttaga aaaatcaacc atagaagaac gcatagatac cgttgttcca
     5881 caagagaatg acggtatttt ttaatttctt taaaaaatct gcaacaaaac atcaactagc
     5941 gtacagatat ggtacgagaa aaggaaatct aaactttttt gaaaataggt cattaaatcg
     6001 aggtttccat tccctatatg aagtgaaaga agaaaagttt tgaaaaatgt cggaaaatcg
     6061 agttaagcgg acagtgttat gtgaaaagaa gaaatttttt tcattctcaa cttaacaatc
     6121 agcagtttcc attccctata tggagcaaag aaaatctttc attccatagt tggcagtaac
     6181 ggtgttgcat gggtagttag ggtgtgaaaa gaaatctttt catcatcaac ttagcagaat
     6241 gacattttct ttccctatat ggtacagaaa aagaaaaaag ttttgagtat tggttacgct
     6301 tttgctcttt tccaacgcgg tatataggaa agacgatttt cttctttcgt gttctttgac
     6361 aattgaataa agcaattcaa tacattagac agacagtaag ccattgaaac agatacgcca
     6421 tgacctttcc cctgcaaaag cgagcgacaa cctatctgtg ttcttgtaaa agacttgtcc
     6481 tcttttatcg ccatgacctg tcctgtctga taatgatact cccgtacagc cacagcttga
     6541 gcgttaaaag cgtcgcacgc aatgggtacg gctacattgg ataatgcaga ggtggaactc
     6601 ctgtgggcta tgacaaaagc tgttgaattg cttagaaaga ttttacagaa aggggggtat
     6661 gtgttattga taatgctgta aaaacaattc aaaagaaaaa taacaagtgt ggcattggaa
     6721 acagaggaaa gacaaagatt gttgtaaaag agcatttttc cgaaaaggga aagacaatgg
     6781 aagaactttt aactgatgtt atgcttgaga aagcaaagca gacaatcgca taaattcggt
     6841 gcagttgtaa gaaatagatt gaatggcaaa aagtacccgt gttatacttt acttgcaagc
     6901 aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa caacagattt
     6961 acaatactgc actttatctt aggttgagcc gagatgatga attacaaggc gaaagttcca
     7021 gcattaccac acaaagaagt atgttgcgtc tatatgcaaa agaacatcat ttgaatgtca
     7081 ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgaaagaccg agttttcaga
     7141 gaatgattga ggatatagag gtaggaaaaa tcaactgtgt tgtaacaaaa gacctttccc
     7201 gacttggcag aaactatatt atgacaggac aatatacaga attgtatttt cccagccata
     7261 atgtccgtta catagcgatt gacgacggtg tagacagcga aaaaggcgaa agtgagattg
     7321 caccatttaa gaatattatc aatgaatggg tggcaagaga tacaagccgt aaagtgaaat
     7381 cagcctttaa gacaaaattt gcagagggtg cgtattatgg ggcttatgct ccgttaggat
     7441 ataagaaaca ccccgacatc aaagggaaac tgttggttga tgaggaaaca aaatggattg
     7501 ttgaaaaaat cttctctctc gcctatcaag gttacggtag tgcaaaaatc acaaaaatac
     7561 tgcgagaaga aaaagtcccg acatcgtctt ggttgaattt tacaaggtat ggtacttttg
     7621 cacatatttt tgagggcaaa cccgaaagta agcgttatga gtggacgatt gcacaaatca
     7681 aaggaatttt gaaaagtgaa gtttatatcg gcaacagcgt tcataatcga cagtcaacag
     7741 tatcattcaa aagcaagaag aaagtgcgaa agcccgaaag cgaatggttt cgagtagaaa
     7801 atacccacga gccaattatt gaaaaagaag tgttctatcg tgttcaggaa cagataaaat
     7861 caaggcgtag acagacaaag gaaaaggcaa cgccgatatt tgcagggctt gttaagtgtg
     7921 cggattgtgg ctggtctatg agatttgcaa caaataaggc aaataagacg ccttacagtt
     7981 attatgcttg cagtttctat ggacaatttg gcaaaggcta ttgttctatg cactatatcc
     8041 gctatgatgt gctgtatcaa gctgtattgg agcgattgca gtattgggct aaggcagtac
     8101 agcaggacga agaaaaggta ttgcataaga tacagaaagt cggtaatgcc gagcgaatac
     8161 gggaaaagaa gaaaaaggca agtgccttga agaaagccga gaaccgacaa aatgagattg
     8221 actgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataaca gaacgaaact
     8281 tcattatgct gtcgggaaaa tatcaaaagg aacagataga actggaacag cagataaccg
     8341 acttaagaga agaattaagt aaaatggaac aggatatgat aggtgctgaa aaatggattg
     8401 agttaatcaa ggagtattcc gtaccaaagg aactgacagc accgttatta aatgcaatga
     8461 tagaaaaaat actcattcac gaagcaacaa cgaatgagga gaacgaaaga atacaggaaa
     8521 tagagatata ctaccgattt attggaaaag tagactgata aatagggttc atatctttaa
     8581 ctaagggaga cggaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag
     8641 tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa
     8701 tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc cattattt
//
LOCUS       DAPBBG010000084         8621 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPBBG010000084 DAPBBG010000000
VERSION     DAPBBG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.087"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            3..1959
                     /locus_tag="RD477_002573"
                     /pseudo
     CDS             3..1959
                     /locus_tag="RD477_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
     gene            1949..3544
                     /locus_tag="RD477_002574"
     CDS             1949..3544
                     /locus_tag="RD477_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDT7634882.1"
                     /translation="MKNKWCKSAVIYQIYPRSFQDTNGDGIGDIPGILSRLDYLENLG
                     INAIWLSPVYESPNDDNGYDISDYRKIGAEYGTMADMDLLIKEARQRGIRIIMDLVIN
                     YTSDRHPWFQLAKKSVTSPYHDYYVWRKEPNGLESNFGGSAWTYVPEVNQYYLHLHSK
                     KQPDLNWQNPKMRQAIYDMMNFWLEKGIGGFRLDVIDLIGKQPEQSITKNGPLLHDLL
                     QEMYQKTFGHYDVVTVGETWGATPKIAQLYSKEERKELSMVFQFEHINLDKQNGKRKW
                     DLKDLDPQELHRTFSKWQIELGGCGWNSLFWNNHDLPRIISRWGDDQEYRTISGKMLA
                     IYLHFMQGTPYIYQGEEIGMINYPVKSIDEVDDIESQRMYAERIKKGYSKEELIQSIN
                     SKGRDNARHPMQWNNHKNGGFTEGTPWLPVGNTEEINVEAALAAQDSLFYTYQKLIEL
                     RKELPVIVYGKYQVIETEEPNVLAYLRSLKKQKILVVVNFSDQKTTFPIGKYRVKQVL
                     IQNYKKKITNNLLPYEAFSALIY"
     gene            complement(3739..4074)
                     /locus_tag="RD477_002575"
     CDS             complement(3739..4074)
                     /locus_tag="RD477_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7634883.1"
                     /translation="MGIEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            4360..6240
                     /locus_tag="RD477_002576"
     CDS             4360..6240
                     /locus_tag="RD477_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDT7634884.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPLTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDDVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVVIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            6319..6546
                     /locus_tag="RD477_002577"
     CDS             6319..6546
                     /locus_tag="RD477_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7634885.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDKVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            6551..7093
                     /locus_tag="RD477_002578"
     CDS             6551..7093
                     /locus_tag="RD477_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDT7634886.1"
                     /translation="MNIVEEKFAQEIKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLLASTILENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            7338..8057
                     /locus_tag="RD477_002579"
     CDS             7338..8057
                     /locus_tag="RD477_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDT7634887.1"
                     /translation="MKKRILLIMGLAFLLAGCTNGNEPRKESTKETAKTTIATTTTTS
                     TESKSTATSTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KAAQLEAFMTTWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFDQIVYGQVNQ"
     gene            8387..>8621
                     /locus_tag="RD477_002580"
     CDS             8387..>8621
                     /locus_tag="RD477_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDT7634888.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPL
                     LVFFLTMASIAKHKIGNKTFVKPILVLYLVGTLL"
BASE COUNT         2824 a         1413 c         1852 g         2532 t
ORIGIN      
        1 aaatggatta caaaaaagta gctgaagaaa tcagcaaagc gcttggagaa gacaatgtac
       61 aagcagctgc gcattgtgca acacgtttac ggcttgtctt aaaagattca gacaaagtag
      121 accagaaggc tctggatgat aatgaaagtg tgaaaggaac ttttgaagca aatgatcaat
      181 ttcagatcat tatcggagca ggtgatgtga ataacgtata tgatgaattc gttaagatca
      241 ccggcgtaaa ggaagcttca accgacgatt tgaaagcggt agcatcgaat aagaagaata
      301 atactgtcat ggcctttatc aaggtgttgt cagatatctt cgttccgatt gtccctgcat
      361 tggttgcggg tggactgttg atggctctga ataatgtttt gacggcacag gatttgtttg
      421 gtcctcagtc agtggttgag ttgtatccat cagtgaaaga ttttgctggc atcattaatt
      481 tacttgcttc agcgccgttt gcttttttgc cgattttggt aggtttttca gcaactcgac
      541 gttttggagg caatccgtat cttggagcag caatggggat ggcgatggtc atgccggatt
      601 tagtgaatgg atacagtgtg acgaatgcta ttgcagaagg atcgatgcct tattggaata
      661 tttttggaat gaatgtggca caagccggtt atcaaggatc tgttcttcca gtccttgcag
      721 tttcttggat tttagctact ttagaaaaat tcttccataa acggttggct aatgctttcg
      781 actacacatt tactccaatg ctagctatta ttattactgg ttttttaact tttacagtag
      841 tagggccggt gatgcgtact gtcggagacg gcttagctga tggattaagc tggttataca
      901 gtactactgg agctttcggc ttaggtgtct ttggtttctt ctattcgccg atagtcatta
      961 ccggattaca tcaaagcttt ccagcaatcg agactacttt actagcagat gttgccagaa
     1021 cgggtggctc ttttatcttt ccagttgcct caatggcaaa tgtagcgcaa ggagcggctg
     1081 ctttggcgat tatgctgctg acaaaaaata aaaagcaaaa gagtctagct accactgcag
     1141 gtgtttccgc tatgttgggg attactgagc cagcgatatt cggtgtgaat ttgaaactga
     1201 aatttccgtt tatttgtgga atgattgctg caggaattgc ttcattcttt atcggcttgt
     1261 tccacgtatt agcagttgcg atgggaccgg caagcgtcat tggctttatt tccattgcac
     1321 ctcgttcgat tccttcattc atgatcggaa ttgttatcag tatagtgatc ggttttttta
     1381 agcacttact tctatgggaa aaaacatatg gaactgctag cagatggtga atcaaaagga
     1441 acagaagcag ctgagtcaat cagttcagct cctaaaacga ctaggaaacc gcaagatgaa
     1501 attttagctg catcagtcag cgggaagatt ctccctttaa cgaaggtaaa agatccagtt
     1561 ttttccactg aaatgatggg aagaggtata gcagtcaaac cagatgacgg ggtagtccat
     1621 gctccagcag atggtatatt gacagttgtc cacgaatcta aacatgcata tggcttgaaa
     1681 acagagaaag gagcggaagt actgatccat atcggtatcg atacagtgaa tctaaaaggg
     1741 gccggattca cgacacagcg acaatatggt gaattagtga aaaaagggga tattctcggt
     1801 acatttgatc gtaaattgct taaagaagaa gggtacgacg atacaattat ggaaattgtc
     1861 actaattcct ctgcatatgc taatgtaaaa attcttgaaa aaaacacggt caaaagtggt
     1921 gaagatattc tgatagtgag tgaaacagat gaaaaataaa tggtgtaaaa gtgcagtaat
     1981 ctatcaaatc tatcctcgaa gttttcaaga tacaaatgga gatggcattg gagatattcc
     2041 tggtattctt tcaagattag attatctaga aaacctggga atcaatgcga tatggttaag
     2101 cccggtttat gaatcaccta atgatgataa tggctatgat atcagtgact acagaaaaat
     2161 aggcgcagaa tatggaacga tggcagatat ggatttactg ataaaagaag cacgacaaag
     2221 gggaataaga atcatcatgg atcttgttat caattatacg tcagacagac atccatggtt
     2281 tcaattggca aaaaaaagtg ttaccagtcc ctatcacgac tattatgttt ggcgtaaaga
     2341 accgaatgga ttagagtcga acttcggcgg ttcagcatgg acctatgtgc ccgaagtgaa
     2401 tcaatattat ctgcacttgc attcgaaaaa gcagccggat ttgaattggc aaaatccaaa
     2461 gatgagacaa gcaatctatg acatgatgaa tttttggctt gagaaaggga ttggcggatt
     2521 tcgattagat gtcattgatc tgattggaaa acagccggaa caatctatta ctaaaaatgg
     2581 tcctctttta catgatttat tgcaagaaat gtaccaaaaa acttttggtc attatgatgt
     2641 agtgacagtg ggagagactt ggggagcaac gcccaagatt gcgcagctgt attcaaaaga
     2701 agaacgcaaa gaattatcaa tggtctttca attcgagcat attaatctag acaaacagaa
     2761 tggcaagcga aaatgggatc taaaggattt agatccacag gaattacatc ggactttttc
     2821 taaatggcag attgaattag gtggttgtgg ttggaattct ttattttgga ataatcatga
     2881 tctcccacga atcatttctc gctgggggga cgatcaagaa tatcgaacaa tcagtggaaa
     2941 aatgctagcg atatacctcc attttatgca aggtactcct tatatctatc aaggagaaga
     3001 gatcggcatg atcaattatc cagtaaagag tattgatgag gtagatgata ttgaaagcca
     3061 aagaatgtat gcagaacgta taaaaaaagg ctattcgaag gaagagttga tccaatcaat
     3121 caactcgaag gggcgagata atgctcgcca tccgatgcaa tggaacaatc acaaaaatgg
     3181 tggctttacg gaaggcactc cttggttgcc agtggggaat acagaagaga tcaatgtaga
     3241 agcagcatta gctgctcaag attccttgtt ttatacgtat caaaaactga tcgaattgcg
     3301 caaagaatta cctgtgattg tctatgggaa atatcaagtc attgagacag aggaaccgaa
     3361 tgttttagca tatctacgca gtttaaaaaa acaaaaaata ctagttgtag ttaatttttc
     3421 tgatcaaaag actacttttc caatcggtaa gtatcgtgtc aaacaagtct tgatccaaaa
     3481 ttacaaaaag aaaattacta acaatttact cccatatgaa gcttttagcg cgctgatcta
     3541 ttaagtggtt ttttctatag atgaatcttt gttatgaggt agatagttca atgaaacagt
     3601 tattcgattt gtttatagaa aacaatttaa tcagcaaaaa gggggacaaa cttttaattg
     3661 tctcccttta attgttagga tggaatatta tcactcttaa acaataagtt aaaaaaatac
     3721 tcgtttacat aaaaaagatt atttagatgc gataatatca tagataaaga aacattcatc
     3781 ataagaaaca gtgatcccaa attctgtttc aatatttttg aaggcctttt ttacatgaaa
     3841 aattgtttca tgagaaggtc ttgtgtcatc ttctgatata attttaagtt catgaccgtt
     3901 tatcaatgta cgctcaatta gacacgctgt atgaatcaaa agttttaaaa gaatggaatg
     3961 agtgaatttt ttgtggaaag tacattccaa ttcttggata aaatccatta agcttttttg
     4021 gatcttattc ccagctaata agtagagata tttgtccact gcttcaattc ccatatcaaa
     4081 aaacgatatt tcttgtgaat gagtggctgc tgcccttttt tgattgttgt tcttcagtcg
     4141 taagatgaca aagcatctta tctttgaata aaaaaataga actacctaag gaagcgtttc
     4201 ggcgcttcct ttttctatat gatcgtttaa tgactgtagg aattcagtat accaaaagaa
     4261 aaccaccttt ttgactaaac ttgatagtgg tcaatttttt atgatggata agtatctata
     4321 cttagtctcg taagaatgaa aaggaaaaga gggagaaaga tgaaatttca aaagtggatc
     4381 caagagcatc gaaatcatat caccgcctta tcagctgtgt tgattatttt tggttttatc
     4441 ggaaaattta ctggatttgt cttgttctat aacattgctc tagccatagc ttctattatt
     4501 gcggtcatac ctattatgat ccatgcgtat caggcagtaa aagtcaaagt tatctcaatt
     4561 gatttactcg ttactattgc tgtgcttggt gcctttatga tcggagaata caaggagtca
     4621 gctatcgtta cattcttgtt tttgtttggt agttatctgg aacaaaaaac attgaccaaa
     4681 acgcgggaat caatcaaggc tttgaccgag atggccccat tgactgccag cgttgtccaa
     4741 gaagatggaa cgactgaaga agtggaaatc gatgacgtgg aagaaggtga tatcgtctta
     4801 gtaaaaactg gtggagctat tcctgtggac ggacgagtca ttgacggaaa agggtatgcg
     4861 gatgaagccg cagtgacggg tgaatcacga caagtcgtca aaatcagtgg agatgacgta
     4921 ttttctggaa cgattttagc agatggctac gtgacgatcg aagcaactaa ggtcggagat
     4981 gatacgacct ttgccaaaat tatcgaatta gtcgaagaag cacaagatac caaatcacat
     5041 gcggaaaaat tcattgatcg tttttctcag tattatactc caaccgtttt acttattgcg
     5101 ttgcttgttg gtctgatcag tcgtgatttt cgtttagcca tcactgtttt ggttcttggt
     5161 tgtcctggcg ctttagtcat tggtgcgcct gtttcgaatg tagcaggaat cggtaatgga
     5221 gcgaaacggg gcatcttagt caaaggcggt gaagcaatgg atactttttc caaaatcgat
     5281 acttttgtct ttgataaaac cggtacattg accaaaggaa ctactgctgt atccactatc
     5341 aagaactata cagaggatga aggggacagc ttagctttag tggctcgatt agaaacgtta
     5401 tccgatcatc ctttggggcg cgccgtgatc cattatgctc aggagaaaga cattgatttt
     5461 caacaattag cagtcaaaga taatcagacg atcaagggac aaggaatcac agctgagatt
     5521 gatggtcata cagtttgtgc gggcaacacc aaattgattg ccgctcacaa actttctctc
     5581 acaccaaagc aagtacaaga tttacatcaa ttacaacaaa caggcagctc ggtcattatt
     5641 gtagtaatcg acaaaaaaat cactcaaatt attggtgtgt cagatgtgat tcgaccagaa
     5701 gtcgctgaac aattagctat gttgaaacaa gcaggagcaa aacatttagt gatgctgaca
     5761 ggtgataatc agatgactgc tgattatgta gctgatatgt taggcattga tgaagtccat
     5821 gctgaattat taccagatga aaaggttcaa tttgtgaaaa aataccaaga acaaggattg
     5881 cgcgttgctt tcattggaga tggaatcaat gattctccct ctttggctgc ggctgatatc
     5941 gggattgcta tgggatcagg aacggatgtt gctatcgaaa cgagtgacgt tgtcttgatg
     6001 caatctagtt ttggatcgtt ggttcatgcc taccgtttgg ctaagaaaac tgttttgaat
     6061 acccgtgaaa atgtagtgat tgccattggt gtcgtcttat ttttactcat cggcttattt
     6121 gccgggttta tttacatggc tagtgggatg ttcgttcacg aagctagtat cttgatcgtt
     6181 atctttaacg cgatgcgttt attgtatttt ggacacgaaa gcaaagagga aaaatcgtaa
     6241 aacttgataa gaatcaattt ttttatggaa gctgtaatct agaataagac tatcaagaaa
     6301 aagggaggaa gaactataat gaaaaaaatg attatgcaat tagatacact agcttgtcca
     6361 agctgtatgc aaaaaatcga acatgcggtt tcgcaacagc ctggggtaag caatacgaaa
     6421 gttttgttta atgcgagcaa aatcaaagca aactttgatc cagataaagt tcaccctgaa
     6481 caattagctg cagttgtgga aaaattaggt tatgaagtcc aatcaacaaa agtaaaagag
     6541 gcataaatca atgaatatag tggaagaaaa atttgctcaa gaaataaaac aagcagatat
     6601 cgaccatcat aaaccaactg ctggcgccat ggtggggcat gtattagcaa atttcaaatt
     6661 agaagaaatg aaattgatcc aagcacaatt gtatgttttg ggtacatctt cgcaagaatt
     6721 aagtaccctt ttttcagaat atgctgatca agaagcgaga tggttcaata aaatctctca
     6781 tgagttagtg attgagaatg aaatcgttcc gtctacttta gaagaagtca tgcgttatgg
     6841 aaaaatttac gaacatggtc aaaacaaata tttactagcc tcaacaatat tggaaaactt
     6901 tgcaaaagat tttgatttcc aaaatgtctt tatcacacgg gcaatcaagt tagctgaaaa
     6961 agaagaaaaa tatggcttgc aacaattact cgttgaactt ttaagtttca ataaagaaat
     7021 catctaccaa gtacaaagac tccaaggaaa aactgtccga caagatttgg atgaggaaga
     7081 cgatgatgac taaaagtcta tgtttcttca aacacaaata taaagcgtga gaaaagcagg
     7141 aaaaaaacac gaagtttttg tcttaaaaat aaaataaaaa gaagcacccg ctgatgttgt
     7201 ctataagaca tcagcgggtg cttcttagtt tttaaatggt ttaaaagatg gatgagaaaa
     7261 tacaaaaaat caataaaatc acattgctta tttgatagaa taaagaagtg ggggagtaat
     7321 cgaaaggggt tttttaattg aaaaaaagga ttttattaat aatgggtcta gcttttttgt
     7381 tagctggttg taccaacggg aatgaaccaa gaaaagaatc aacaaaagaa acagcgaaaa
     7441 caacaattgc aactactaca acaactagca cagagagcaa gtcaactgca acaagtacag
     7501 aaacagcaga tacctctact tcgtcagagt cttccaaaac gactgaaaca actcaaacat
     7561 ctacttcaag ttcttctgta gaggcttcat cttcctcttc agaaagcagc gaacaagtcg
     7621 aacaaacact gtggaatgca gacaaagccg cacaattaga ggcatttatg accacatggg
     7681 gccaaacaat gggacaacaa tacaaaagtt atacgaatca aatgagtgtg gacttatatg
     7741 gtcttaaagt ccctcaagct attttgaatg gggagtggaa gatggctatt ggtggagtgc
     7801 ctgtttctgc agaatggtca gaaagcggga caggtcaagc agattatcaa atcacagccg
     7861 tctattccga tgcagagaca gaaccatatc tgaaaaagca cgtctatcta ttcggttttc
     7921 agcaaaatca gccaaaagta ttggtgacac aacaaaatca ggggaatccc gataattatc
     7981 tttacttcaa cgagacagcg aataatgaat tgaaaaatgg ctttgatcaa atcgtttatg
     8041 ggcaagtaaa ccagtgagag tgagagaaag aagagactca gtggaaaata ttaattgatt
     8101 gttatgtcaa agaattaaga tgaaggatga aatttctagt ttcgtccttc atcttagtta
     8161 cgttttactt ggataacttt aataataacg ataaatagtt ttctctcgac ttctttagag
     8221 attgaataaa ccctccaata ctaacattga atgttgcaag agcatgaatt gccaattgta
     8281 ttattagaat ttttagaaaa ttgcaccttt agtttcttta gataaaatga tacaatgatt
     8341 ggtaaaattg tgaaagggtg gtcatttgtt agagaagttt ttggatatgt cgttagttca
     8401 acgtattgtt gttggtattt tagtcggaat attgtttggt atctttttcc ccacgtggac
     8461 atttatttca atattgggta cgttgtttgt cagttgcttg aaagcaattg cgcctcttct
     8521 agtttttttc ttgacgatgg cttcaattgc caaacataaa ataggaaata agacatttgt
     8581 gaagcctatt ttagtcttat atttagtagg aacattgctt t
//
LOCUS       DAPBBG010000085         8273 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPBBG010000085 DAPBBG010000000
VERSION     DAPBBG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.088"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(4..807)
                     /gene="msrA"
                     /locus_tag="RD477_002581"
     CDS             complement(4..807)
                     /gene="msrA"
                     /locus_tag="RD477_002581"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7634889.1"
                     /translation="MDKEQVFIQTLYNLILNPNTRDWERKVLIQTKNDIRENTSAKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLLTEGFDTQQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGNLSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SDLLTIYWQITDPTDAFGQFQDRGKQYRPVIFYQDERQKKLAEQSKQELVSSGTFRQP
                     IVTKIEPVGTFWPAENYHQQFYKKQPKRYKKIQQARKQFLIYQRIKNKW"
     gene            complement(1042..1785)
                     /gene="truA"
                     /locus_tag="RD477_002582"
     CDS             complement(1042..1785)
                     /gene="truA"
                     /locus_tag="RD477_002582"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDT7634890.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNSEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHA
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLMGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(1921..2658)
                     /locus_tag="RD477_002583"
     CDS             complement(1921..2658)
                     /locus_tag="RD477_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7634891.1"
                     /translation="MFKYMDKIKQLLDLVEKEEKKSIKEAIDVLTQANLQKRSVYIFG
                     ASHAGILAEEMYYRAGGMMTINAIFGREVMLDRSPITFTSQMERLEGYGTNLAKTVSF
                     KNQDVLILHSVSGRNPIIIDLALTAKAKGVKIISLTNVQYSRSVTSRHSSGKRLFEVS
                     DIVIDNHGDIGDACCELTGIVQKAGPSSTVIGAAILNTIIVEVCEQLIANGIKHPPIF
                     YSANLDGGDLRNAELYEEYKEVIHYEL"
     gene            complement(2659..3909)
                     /locus_tag="RD477_002584"
     CDS             complement(2659..3909)
                     /locus_tag="RD477_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDT7634892.1"
                     /translation="MNFISEHILKNPPVLLGLIAMLGLFIQRKSFSETVKGALTAAFG
                     MVALTAGVNMLVGTISPINIAVQTQLGVEVTEGLSDVTFTAEYGGTVGLAMFLGLVIH
                     LLIARFTPVKTIFLTGHMLWWFPFVFVAAGVEAGLSGGALIGISGILSACYWSFMPWI
                     MRKYVWDATGDDSFLIGHPTGVLSLVSGFVAKRVGNKEKSTEDIKVPENLSFFREISI
                     TGALVMFLMNTVVGIIAPVLIPEGDNLLMFSIEAGLNFGAGLLIMLYGVRLLINQIIP
                     AFQGIAEKVVPGAKPAFDVPILFNYRPNAVIIGFIVAMITSTILVVLANSFHLFGVLL
                     VPLVITSFFECGGAAVIGEGQGGFRGAVVGTIAASFVMVVLAGFSAIVFSTTIQSWIL
                     IFGGNDLSLWGMIGRGFSGLFGGF"
     gene            complement(3914..4195)
                     /locus_tag="RD477_002585"
     CDS             complement(3914..4195)
                     /locus_tag="RD477_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7634893.1"
                     /translation="MKILAVCGFGVGSSMVLKMSLDKVIKELGLKATVENTDLSTAKA
                     TMADVYFTSHELLPELKASVKAPVYPIKKYMDTSEIKAQLEQYLADKGE"
     gene            complement(4208..4639)
                     /locus_tag="RD477_002586"
     CDS             complement(4208..4639)
                     /locus_tag="RD477_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7634894.1"
                     /translation="MSTINKLLPKNQIQVIETAENWMKAVEIASNPLLKNKMITHTYL
                     ENMIRSVEENGPYMVLADYFALMHAKPGLGVNEQSMSLLVVKDAVDMKGKPIHIFLVL
                     AAKNHESHLERLKDIMEIFMDNEKYQTILSGNKETIIQLFA"
     gene            complement(4636..6276)
                     /locus_tag="RD477_002587"
     CDS             complement(4636..6276)
                     /locus_tag="RD477_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7634895.1"
                     /translation="MSVIQYQESKLRRVAILNRLSNFEHVSYQQLSEEYYVSRSSIAN
                     DISYIKYVFAKEGLNLSFDRFGTYFEGNEIQVQKVLKRMILNHFDELEIVREIVDRKL
                     LAIVERSFRESIADKQIELPESYFSSIIVSILLMIQRSREGKKISLKGKNQYGKYFLE
                     FNKYPLVCELLHQLEEQRIYQFSQEEVQYLTYVIVGSGLRFFMKNETIPLVFREKIRQ
                     LIQKVSRGIQIDLTQDSRLEEDLLVHLYQLLLRIEAQTTIVNPLIDEIKQNYPSLYGV
                     VWFALNDFRWPYQVSLSEDEVGFVAIHFQAAIERVKRLNRLLFVCPNGMGTSSFVSAK
                     IRRILPDIDSIETASVDKLNRLDLFDVDLIISTVDIPKQIRPVVRISPMVTARDMKRI
                     MNHYIDLVIDNEQINIEGSIAEKVKPLISSNVYFDHFDSKEAAIRFLVDQQQFENEES
                     KQRFLYSIFEREEIQSTYLENGFAIPHGNPNVVKQTSVAVLIADKPFSWGYQKADIVV
                     LLMIREEDVKEVEPVMKMVMQGIEDKNWFISKMLEVRE"
     gene            complement(6496..7131)
                     /locus_tag="RD477_002588"
     CDS             complement(6496..7131)
                     /locus_tag="RD477_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDT7634896.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EISSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTASIGFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWSFLNKIGFCQRISFFFNYVPIAWLSILHLRQ"
     gene            complement(7283..>8273)
                     /gene="sstT"
                     /locus_tag="RD477_002589"
     CDS             complement(7283..>8273)
                     /gene="sstT"
                     /locus_tag="RD477_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDT7634897.1"
                     /translation="LSALVAVIISSIFTVPITLQETVSEQAPQNLESVLSTMLTNVTQ
                     NPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQISVALSQVVQVIIAFAPIGI
                     LGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLILFCL
                     KESALPAFFTRSSAANIPINMMLAEKLKLTKESYSISIPLGATINMGGAAITISVMSL
                     TAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIV
                     GIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDRLDGLNER"
BASE COUNT         2559 a         1794 c         1337 g         2583 t
ORIGIN      
        1 tttttaccat ttgtttttta ttcgttgata gatcagaaat tgtttacgtg cttgctgaat
       61 ttttttatag cgtttcggtt gttttttgta aaattgctga tgataatttt ctgctggcca
      121 aaacgtacca actggttcaa tttttgtgac gattggttgg cggaacgttc ctgaactgac
      181 tagttcctgt ttcgattgct ccgctaattt tttctgacgt tcgtcttgat aaaaaatcac
      241 cggacggtac tgcttccctc tatcttgaaa ttgtccaaag gcatctgtgg gatcagttat
      301 ttgccaatag attgtcaaca aatcgctata acttatctct ctcgtatcat aaatgatctc
      361 cacagcttcc acatggcctg tgtaaccacc acttacttga tcataatttg gtttttcaac
      421 atgtccgccc gtgtatcctg acaaaacgga taggttccct tttttcgatt caaacggctc
      481 gaccatgcac cagaaacatc cccctgcaaa tactgcacgt tcttgatagt cagcatcttg
      541 catagcatgc ttggaaaagt catatttttg ttgcgtgtca aatccttccg taagcaataa
      601 ataaaagtcc attacgtcag gcgtcagatt cattcggata gctaatggac gtagtgttgc
      661 ttctagtttt gataactgtt ctttagcgga tgtattctct cttatgtcat tctttgtctg
      721 gatcaatact tttcgttccc aatctcgtgt attcggattt aaaatcagat tataaagtgt
      781 ttgaatgaat acttgttcct tatccataga ctctcctcct ttccgaagaa gacaagctag
      841 tgactttagc tgtaatttat cataaagtga ggaaataagc gattgaatcg tatctattca
      901 aagaagataa gcggcttcag atgatataag atgatcttta tcttaaaaag tatactctat
      961 ggtttggttt aactaaaaaa cgacctctga aaaaagggat gttctccagt tctcagaagt
     1021 cgagtaaaga tgattctttt tttatgaacg ataataaact tcatccaaaa acagaccttg
     1081 tgcagggacg gtttctcctg catgctgccg cactttgctt tcgaatattt catcgattgt
     1141 ttctatcggc atggcacctg taccgatttc tagtaatgtt cccatgatga ttcgcaccat
     1201 tttatataga aatccgtctc cgacaaaagt aaaatgaagc atttctccct tacgttcgat
     1261 agataattca tcaatcgtac gtaccgtaga tttttttgat tttttcaatg cagaaaaacc
     1321 aatgaaatca tgtgtaccca tcaatttttc gcaagctgca ttcagcttat ccatatccaa
     1381 ctcttgagga aaataaaagc tgtgatttcg ttcaaatgct gaaggaatca cgttgttcca
     1441 aacataatag ctgtattttt tgcccaccgc attatatctt gcatggaagc gctcaggcat
     1501 ctcttcgatt cttttgatga caatatctcg cggaagatag cgattcataa agtcgatcat
     1561 ttctgccagt ccaatttctg agtttgtttt gaaatttgcc acttgtcctc gcgcatgcgt
     1621 gccggcgtct gttcttcctg atccaataat ttctatctct tcatttgtca tctgatgcaa
     1681 cactttttcg attttccctt ggatggtttt atcgtcatct ccaagtcttt gccagcctga
     1741 atatcttttt ccgtcatatt cgatcgtcaa cttgatgttt ctcatatttt tgtggctcct
     1801 ctatattcca tttggtcttc tctctacctt tttaagattg tagcacaaat taaagctact
     1861 tgcttctctg tgagagaaac caagtagctt taaataattg tttatgaaca attgtattat
     1921 ttataattca taatgaatca cttctttata ttcctcatat aattctgcgt ttcgcagatc
     1981 gcccccatct aaattcgccg aataaaatat aggaggatgt tttatcccat ttgcaataag
     2041 ctgttcgcaa acttccacga tgatcgtatt gaggatggct gcaccgatta cagtagagga
     2101 aggaccggct ttttgtacga tacctgtcag ctcacagcaa gcatctccta tatccccatg
     2161 gttatcgata actatatcag aaacctcaaa caatcgtttc cctgatgaat gacgactagt
     2221 gaccgatcgt gaatattgga cattcgtcaa actaatgatt tttacgccct tcgcttttgc
     2281 agttaacgct aaatcaataa tgattgggtt tcttcccgat accgagtgaa ggatcaatac
     2341 atcttgattt ttaaatgaaa ctgtttttgc taaattcgtt ccatatcctt ctaaacgttc
     2401 catttgactg gtaaaggtga taggggaacg atctagcatc acttctcgtc caaagatagc
     2461 attgatggtc atcatcccac ctgcccgata atacatctct tccgctaaga taccagcatg
     2521 actagcacca aaaatataga ccgaacgttt ttgcagattc gcttgggtca atacatcgat
     2581 tgcttctttg attgactttt tttcttcctt ttcaaccaaa tctaataact gtttgatttt
     2641 atccatatat ttgaacatct agaaccctcc aaacaaaccg ctaaatcctc taccgatcat
     2701 tccccataac gacaaatcgt ttccgccgaa aatcaatatc cagctttgga tcgtcgtact
     2761 aaaaacaata gctgaaaaac cagctaatac gaccatgaca aatgaagctg cgattgttcc
     2821 gacaaccgct ccacgaaagc caccttgacc ttcaccgatg actgctgcac caccacattc
     2881 aaagaaactt gtgattacta atggcacaag caacacgccg aatagatgga atgaattcgc
     2941 caaaacaact aaaatcgtag aagtgatcat tgccacgata aaaccgataa tcactgcatt
     3001 tggtcgataa ttgaataaaa taggcacatc aaatgctggt ttcgctcccg gtacgacttt
     3061 ttctgcaatc ccttgaaacg ctggaatgat ttgattgatc agtagtcgga caccatatag
     3121 cataatgagt agtcctgcac caaaattcaa ccctgcttca atcgaaaaca tcaggagatt
     3181 atccccttct ggtatcaaaa caggtgcgat gatcccaact acggtattca tcaagaacat
     3241 gactagtgca cctgtaattg agatttctcg gaaaaatgaa agattctcag gtactttgat
     3301 atcttctgtc gatttttctt tatttcctac tcgttttgct acaaatcctg aaactaaaga
     3361 caaaacaccc gttggatgac caatcaggaa agaatcatct cccgtagcat cccagacata
     3421 tttgcgcatg atccacggca taaaagacca ataacaagcc gataaaatcc cgctgatccc
     3481 aatcaatgct ccccctgaca gtcctgcttc cacaccagct gctacaaata cgaaaggaaa
     3541 ccaccaaagc atatgacctg ttaaaaagat cgtcttcaca ggtgtaaacc gagcaatcaa
     3601 taaatggata actaatccta aaaacatagc taatccgacc gttccgccat attctgcagt
     3661 aaacgtcaca tcagatagtc cttctgtcac ttctacaccc aactgtgttt ggacagctat
     3721 attgattgga gaaatcgttc cgaccagcat attgactccg gcggtcaaag caaccatacc
     3781 aaatgcagcg gtcaaagctc ccttaaccgt ttctgaaaat gattttcgct ggatgaacag
     3841 cccgagcatc gcaatcaaac ctaacaagac gggcggattc ttcaagatgt gttcactaat
     3901 gaaattcatt cttttattcc cccttatcag ctagatattg ttccaattgc gcctttattt
     3961 ctgaagtatc catatacttt ttaataggat aaacaggcgc ttttacagaa gcttttagtt
     4021 ctggaagtaa ttcatgactt gtaaaataaa catctgccat cgttgctttg gcagtggata
     4081 aatctgtatt ttccactgtc gcctttaaac ccagctcctt aattacttta tcaagagaca
     4141 ttttcaaaac catagaactc cctacaccaa atccacatac tgctaaaatt ttcatttttc
     4201 ttcctcctta tgcaaataac tgaatgattg tttctttatt tcctgataaa atcgtttgat
     4261 atttttcatt gtccataaat atttccatga tatcttttaa tctctccaga tggctttcgt
     4321 ggtttttggc agctaaaacc aaaaaaatat ggattggttt ccctttcata tctacagcat
     4381 cttttacgac taaaaggctc atactttgct cgtttactcc aagacctggt tttgcgtgca
     4441 tcagcgcaaa ataatcagcc aataccatat atggtccatt ttcctctaca cttctgatca
     4501 tgttttctaa atacgtatgc gttatcattt tgttttttaa taatggattg ctagcgattt
     4561 cgactgcttt catccaattt tcagccgttt ctattacttg gatttgattt ttcggcagca
     4621 atttattgat agtactcatt ctctgacctc caacattttt gaaataaacc aatttttatc
     4681 ctctattcct tgcatgacca ttttcatcac aggttcgact tctttcacat cttcttctcg
     4741 gatcattagc agaacaacga tatctgcctt ttgatatccc catgaaaatg gcttgtctgc
     4801 tatcaataca gcaaccgaag tttgtttaac gacattggga ttgccatgag gaatagcaaa
     4861 accattctcc aagtatgttg attggatttc ctcacgctca aaaatagagt agagaaatcg
     4921 ctgtttcgat tcttcattct caaactgttg ctgatcaacc aaaaaacgga tagcagcttc
     4981 ttttgaatca aagtgatcaa aatagacatt agaggaaata agaggtttca ctttttcagc
     5041 tatcgagcct tcgatgttta tttgttcgtt atctatgacc agatcgatat aatgattcat
     5101 gatacgtttc atatctctag cagtaaccat tggcgaaatt cttactactg gtctaatttg
     5161 tttaggtata tcgacagtgg agataatcaa atctacgtca aacaaatcta gtctgttcaa
     5221 tttatcgaca gatgctgttt cgatactatc gatatcaggt aagattcttc tgatttttgc
     5281 agaaacaaat gaactggttc ccattccatt gggacatacg aataagagtc tatttaatcg
     5341 tttgacacgc tcgattgctg cttggaaatg gatagctaca aaacctactt catcttcgga
     5401 caagcttact tgataaggcc agcgaaaatc atttaacgca aaccagacaa caccatacaa
     5461 agaaggataa ttttgtttta tctcatctat caaaggattt acgatggtcg tttgagcctc
     5521 aatacgtaaa agcagctgat acagatggac aagcagatct tcttctaaac gactatcttg
     5581 ggtaagatct atctgaatgc ctctactcac tttttggatc aattgccgga ttttttctcg
     5641 aaatactaaa gggatcgttt catttttcat gaaaaagcgt aaaccacttc ccacaataac
     5701 ataagtcaaa tactgtactt cttcttgcga aaattggtaa atacgctgtt cctctaactg
     5761 atgcagcaat tcacaaacga ggggatattt gttgaattct aaaaaatact ttccatattg
     5821 atttttacct tttaaactga ttttcttccc ttctcttgaa cgctgaatca tcaataagat
     5881 tgaaacgatg atacttgaaa agtagctttc tggtagctca atttgcttgt ctgcaatgct
     5941 ctcgcgaaaa gatcgttcga ctattgctag aagctttcga tccactattt ctcttacgat
     6001 ctccaattca tcaaaatgat tcaggatcat acgcttcagt accttctgaa cctgtatttc
     6061 atttccttca aaatatgtcc cgaaacgatc aaaactgaga ttcaaccctt ctttcgcaaa
     6121 tacatatttg atgtaagaaa tatcatttgc gatactgctt ctggaaacat aatattcttc
     6181 agaaagttgc tggtaactta catgctcgaa attggacaat ctgttcagga tcgctactct
     6241 tcgtagtttg ctttcttgat attgtattac actcatttcc atcaccttct atattcacta
     6301 taattttttc ttataaggag aactagacca aataaaagat taaaataacc tgagaaatca
     6361 tacactgctc tttctcagtt aatatacgct aaaacagaag ctatacaaga aaattatgga
     6421 actatatttc agaaaaacaa aaaaacagcc aacggacgaa atgtccgctg actgttcaat
     6481 ttgatggctc aagttttact gtctgagatg caagatactc aaccaggcaa tgggcacata
     6541 attaaagaaa aaactgattc tttggcaaaa tcctatttta ttcaggaatg accaagaatt
     6601 tatagaagga attcttgcca gaccataaat cgccgcaaaa agcaaactga tcacagacaa
     6661 tccaaggtat aagtggctta cgtccatttg cccatttttt cgatagatca agaaaagaag
     6721 aactggaaac aataaaaaac cactataacc taaacctgat ccgatattat ggacccatgt
     6781 cgagacattc cacgaagatt catgggtatt gatactgaac aaacctgtaa agatacaatc
     6841 acctatacca taaaaaccga tagatgctgt aaccagggta gctaacacac gagatgtact
     6901 tacaaatgca gcatacaatg caggcaatga caggatgaaa aaaatccctg aaaccactga
     6961 ccaaataaga aatgcttttc ttacaggact ggaaatctct ccaaatacac taatcacact
     7021 cttcatttga tctaattcag gataatacaa tcctaatacg tacggagtca aaaaatcact
     7081 caacacgcct aataataaaa agaagaaacc aaatttacgt aaaaaatcca tgatgcgcct
     7141 ccaataacaa ttacctatgc ttttaattgt tattataaac gactttatct cgatttaaca
     7201 ggatcctcta ttttacacga acatctacat aactcattaa aaatttgaat cgtcttaagg
     7261 ttcatcccta aatatctaat attcaacgtt catttaaccc atctaatcga tctttcaatt
     7321 tgattttttc tccacttctt ttacgcgcgc ctaattctcc tattgcagtg aacaacagat
     7381 cacttgagga atttaaggca gtttccattg aatcttggat aacaccgata ataaatccga
     7441 taccgacgat ttgcatcgca acatcattag agataccaaa caaactacat gctaatggaa
     7501 tcaataacag tgaaccgccc gcaacacctg aagccccaca agcagctaaa gcagacaaaa
     7561 tacataaaag gactttcaaa acaaatggtg cttcaatccc taaggaatgt acagccgtca
     7621 atgacatcac ggaaatggta atcgcagcac cgcccatatt gatcgtagca cctaatggga
     7681 tcgatatgga ataagactct ttcgtcaatt tcaatttttc agccagcatc atgtttatcg
     7741 ggatatttgc ggcagaactt cgggtaaaga aagcgggaag cgcactttct ttcaaacaaa
     7801 aaagaatcaa tggatagggg ttttctttaa taaacaaaaa cgtgaggaac gggtacacaa
     7861 ctaatgcgac aaacagcatc gtgccaatca acacaagtaa aagctgcaga tactctgcca
     7921 atccagcgat tccagtcgtg gcaatcgatt gataaaccaa tcctaaaatc cctatcggag
     7981 cgaatgcaat aatcacttgt acgacttgtg ataatgctac agatatttga tccaccaatt
     8041 cttttgtcga ttctgaacgt gctcgaaaag ccagacctaa caacacagcc cagaataaga
     8101 caccaagata attcgcatca atgagcgatt gaacaggatt ttgtgtgaca tttgtcaaca
     8161 tcgtagataa aacactttcc aaattttgcg gtgcttgttc agatactgtt tcttgtaaag
     8221 taataggaac agtaaaaatt gaacttataa tcactgctac aagtgccgaa agc
//
LOCUS       DAPBBG010000086         8271 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPBBG010000086 DAPBBG010000000
VERSION     DAPBBG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.089"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     regulatory      123..202
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            200..1393
                     /locus_tag="RD477_002590"
     CDS             200..1393
                     /locus_tag="RD477_002590"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDT7634898.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1523..2995
                     /locus_tag="RD477_002591"
     CDS             1523..2995
                     /locus_tag="RD477_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634899.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3078..3866
                     /locus_tag="RD477_002592"
     CDS             3078..3866
                     /locus_tag="RD477_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDT7634900.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3933..4601)
                     /locus_tag="RD477_002593"
     CDS             complement(3933..4601)
                     /locus_tag="RD477_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDT7634901.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4716..6428
                     /locus_tag="RD477_002594"
     CDS             4716..6428
                     /locus_tag="RD477_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634902.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6431..8203
                     /locus_tag="RD477_002595"
     CDS             6431..8203
                     /locus_tag="RD477_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7634903.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2530 a         1385 c         1818 g         2538 t
ORIGIN      
        1 gttatcttta tggatgattg tgctaagctt ttttggacag ttctaatgta aaatattttt
       61 atatttttta agcaaaaaaa tgaaaaaata cgagcagaaa actgttttat ctgctatact
      121 tggttcgttg caagttcccg aaaggatccg caggacggag atgccttgta accgaattta
      181 actaggggga atcattataa tggtagaaag acacttattt acatcagaat ctgtttctga
      241 aggacatcca gataaaattg ctgaccaaat cagtgatgca atcttggatg caattttaaa
      301 acaagatcca acagcacgag tagcgtgtga aacgtctgta acaactggtc ttgttttagt
      361 atttggagaa atatctacga cggcatatgt agatattcaa aaagtcgtac gggaaacaat
      421 aaaagaaatt ggttatacac gtgcaaaatt cggatttgat ggagatacag cagctgtatt
      481 ggttgcgatt gatgaacaat ctcctgatat tgctcaagga gttgatgaag cacttgagat
      541 ccgggatgaa gataaaaaag acgtattaga tgagattggt gctggtgacc aaggtttaat
      601 gtttgggttt gctgtagatg aaacaccaga attgatgcct ttaccaatcg ctttgagtca
      661 tcgtctggta cgacgcttgg cagatttgcg taaatcaaat gaattaacgt atttacgtcc
      721 agatgcaaaa tctcaagtaa cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga
      781 tacaatcgtt atttcaacac agcatgatga tgcagtagat aatgaaacaa ttcgtcatga
      841 tgtcatcgaa aaagtagtga aggaagttat tccagctgaa ttattagatg atcaaacgaa
      901 atattatatc aatccgactg gccgatttgt cattggtggt cctcaagggg atgccggatt
      961 aacaggaaga aaaatcattg ttgatacgta tggcggttat gctcgtcatg gtggcggtgc
     1021 tttttctggt aaagatgcga caaaagttga ccgttctgcc agctatgctg cacgttatat
     1081 tgctaaaaac attgttgcag cagggcttgc tcgcaaagca gaagtacagc tagcttatgc
     1141 gattggtgtt gctcaacctg tttcgatctc aatcaatact ttcggtactg gaacggttcc
     1201 ggaagaagaa ctgattgctg cagtgagaga aaacttcgat cttcggccag caggaatcat
     1261 cgagatgctt gatttgcgtc gtccgattta caaacaaaca gctgcttacg gtcattttgg
     1321 tcgtacagat gtagatttgc cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag
     1381 tttagcaaaa taagttatat tcagctggaa cgatcgccaa gtggtggttg ttccagcttt
     1441 ttttatggca gttaacctct tgaaaaagat agtaattatt tatctatttt tagatcctgt
     1501 cataatgaaa gggaggaata agatgaaaaa agaaacaaat gttactttgg tcacagtgag
     1561 tattttcatt gcaacgttca tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc
     1621 aacgattgtc ggttcattac atggaatgga aatcatgaac tgggtatttt cgatttattt
     1681 gctgacaaac gctatgctta ctccgattta cggaaaactt gcggataaag tgggacggaa
     1741 accaatcttt atttttggga cattattgtt tattatcggt tcgttatttt gcggtttatc
     1801 tcaaagcatg attgggttga ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat
     1861 gatgccagta gctttgacga tcatcggcga tctttattct attgaaaagc gagcaaaagt
     1921 tttaggtttg aatagttcag cctgggggat tgccagtgtt tttggaccgc ttgcgggagg
     1981 attcatcgtt gaaacgatca gctggcactg gatctttttt atcaatgtgc cgatcggaat
     2041 tctattaatt attttgattt ggctatattt aaatgaacca aaaatcatac atgagtctaa
     2101 accaatggat atttttggta gttttagttt gatggccatg cttgtctcat tgctgttagg
     2161 gttccaatta ttaggagacg aaggaatgag tccgcttgtt tctgctttgc tgctaggtag
     2221 tatcgtaatg ttaggtattt ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat
     2281 gatgcattta ttcaaaaatc cactatttgt tgtggtcaac ttggctgcag cattggctag
     2341 tggttttttg atgggaatcg atgtttatat ccctatgtgg atgcaaggtg tattagggct
     2401 gaatgcagca attggtgggc tggtattagc accattatca ctagtctgga tggtcggttc
     2461 tttccttgca agcaagtgga tgactcaaat gaaaactcat tgggtcttga gaattggact
     2521 actaatcgca gctattgggg gcgcttggtt gtatcttatg cctttcagaa gtaactattt
     2581 atggtttttt gctatatcaa cggtcatcgg tataggctta ggtgtgacca tgactacctc
     2641 gactgtcagt gcacaaaata gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa
     2701 tacgttagtt cgaacaattg gtcaaacagt catggtttct atttttggtt tattgataaa
     2761 tggggtaact aaacgagaac ttgcagcagc aaatttaaca aatgatgcag atatcatgaa
     2821 taaactggtg aatccacaaa cggctaaata tattgatgcg gatattttgt taccattaag
     2881 aaaaattctt tatgatgggt tacattccgt ttatctagtt ggcttgttac tcgtgatcat
     2941 tgctttggtc ttaacgcttt tcgttcatga aaaaagtggt aaaataaaac agtagtttcg
     3001 gttaagatat gtacgtagcc gaatcattca aatttaaagt tattcaaaaa taacttctta
     3061 ctaaggaagg cgaaaatatg gcatttattc aagcaaatat ttattcgaat gtgttagaaa
     3121 tggaagtcaa tctaaatgtg atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt
     3181 cagagcaaga gacaacggat atacctgttt tatatttact tcatggaatg ggtggcaatc
     3241 atagtgtatg ggaaagacgt acgtcgattg aacgttatgt agcaaactac ggattagctg
     3301 tcatcatgcc atctactgat cttggctggt acacagatac agtttatgac atgaagtatt
     3361 ggacattcat tgcagaagag ctacctgaga tttgtcatga attattcccg caattaacaa
     3421 gaaaaagaga aaaaacatat gccgctggat tatcgatggg cggatatggc gcgttaaaac
     3481 taggccttgc aaaatcagaa aatttcgcag cagtaatctc tctttcaggc gctgtttctg
     3541 taggaaatcg aatggatgat cttttgatgg ttcgtaaagc atctttttgg gaagggatct
     3601 ttggaccatt ggatcaaatc gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac
     3661 tagtaaaaag cggaaaacca gttccaagat tttatatggc ttgtggggaa tctgattttc
     3721 tttatcaggc aaatcaggaa atggtatctg ctatgcagga acaaggcttg gcggtcacct
     3781 ttgaacaagg acctggcgat cataactgga tcttctggga ccaatggatt caacgtgcat
     3841 tggcgtggct aatggaagaa aaataaaaaa agagtcttaa aaagatagct cctatagaga
     3901 tcacaaacat agtgatctct ataggagctt ttttactgct cgttttctct tcttttattt
     3961 tgttggcata agccttcaat ataggtttgt gcactcgtta gaaagagtgc tttttcattc
     4021 aattctgcat gggactgcat actttcttta aatccctcta attgattttc aactacataa
     4081 ttacgcataa atagaactgt ttctttcaaa aattcatttc cgtccataat tttttgtcga
     4141 agctgctgtt catgctgctg atccattaac tgtccaatca ataaattgtg gagagaggca
     4201 atggcctgat gactttgggt gactgaaaat ccttcacgaa caagtaattg gatcatttgc
     4261 tccaaatgtt ctaatcgact cggaaaagcg ggcacagtat tgatttctaa ttctgcccca
     4321 caagggaaac gtgtataaag gtcatagtaa ttttccatat actgcagcaa ctgttcattc
     4381 caaggcaaag ttggatctgg aagcgctaat tcttcttcca ttgtttcagc cattttttgc
     4441 aagagctgtt gtttgttttt gaaataccaa tataaagcag gtgcttgaac atgaattctg
     4501 tttgcgatgt ttcgcatgga aagctgcgaa agagcttgtg tttcagccag gatatcaaag
     4561 gcggcctgaa tgatgattgc ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt
     4621 gcattatctt tttaaacatg gtaaattgtt agtcggtcta attttacagt gttaaattag
     4681 tcctgtcaat ttaaacaaag gggtgtaatc aacgaatgaa attaatctta cgacatgcga
     4741 aaaaatataa agtagctgtt tttatttcct tgctgtccgt agcagtcatg gttgcagcag
     4801 cattatggca acctaagtta ctacagcaag tattggaagc tattattaca gaagataatg
     4861 atgaaatgaa aacaattggt atttacctga tcagtctggc tttgctagga ttagccgcag
     4921 gtgtaacgaa cacgattttc tctgcaaaag tagctcaagg cgtaagtgca gatatccgag
     4981 aagaagcatt tcgaaagatc cagacatttt catttggaaa tatcgaacag ttctctgctg
     5041 gaaatcttgt tgttcgtcta acaaatgata tcacgcaaat ccaaaattta gtgatgattt
     5101 ctttgcaatc actttttcga attccattct tatttattgg tgccttcatt ttagctatga
     5161 tcacgatgcc gcaattatgg tggatcatcg tagcgcttat cgttacggta tttctgatta
     5221 cggcactttc atttacaagg atgggcaagc acttcatgat cattcaaaaa ctgattgatc
     5281 gtgtgaatag cattgcaaaa gaaaacttac tagggattcg tgtagtgaaa tcttttgtac
     5341 aagaagaaaa ccaattgaag tcattcagca aagtaagtga ggacttaaca aaacacaaca
     5401 tcatagtcgg tacattattt tctgtgatga tcccgtcctt catgctggct gccaatttgg
     5461 ctgtggttgg ggcgatcttt tttgtgagcg atcttgttaa agatgatcct acgttaattg
     5521 gtggaattgc ttcattcatg aactacttga tgcagattat gatggcaatc attattggcg
     5581 gaatgatgat gatgatgact tcaagagcag ccgtatcgct gaagcggatt gcggaaattt
     5641 tagatgctga accagatctt acttatttag acgttccaga acaagagttg acgggttctg
     5701 tcacttttga ccatgtttct tttcgttatc ctggtgagga tacagatacg cttaaagata
     5761 tcagcttttc tatcaaacct ggagaaatgg ttggtatcgt cggggcgact ggtgccggga
     5821 agtcaacact agcacaattg atccctcgtc tatttgatcc gacagaaggc aaagttgaag
     5881 taggtggtgt agatcttcgt caagtaaatg aaaagagttt aagacaaacg gtttcctttg
     5941 ttttgcaaaa agcaatttta ttctcaggaa cgattgctca aaacttgcgc caagggaaaa
     6001 agaatgcaac agaaaaagag atggaacatg cttcttctat tgctcaagca aaagaattca
     6061 tcgaaaagtt atctgatcgt tacgaagcac caatagaaga acgtagcagt aacttttctg
     6121 gtggtcaaaa gcaacgttta tccattactc gtggcgttat cggtaatcca aagatcttga
     6181 tcctagatga cagtacgagt gcactggatg ctcgctctga aaagctggtc cgagaagcac
     6241 tcgatcgaga tttgaaggga acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg
     6301 tcaaagcaga ccgcatctta gtattggatg aaggcagatt agtcggcgaa ggaacccatg
     6361 aggaattggt agctggaaac cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg
     6421 aggagtaagt atgactgatc taataaaagc aagtaaattt ttctatcact atttgaagcg
     6481 gtacaaactc tcctttttct ttatctttat cacagtgatc attgctacct atctacaagt
     6541 aaaggcacct caatacatcg gggaagcttt tcaagaatta gctaattacg taggaggctt
     6601 gatgcaaggc gtagacgata aaagcaaatt tgtttctgta atatggaaac tcttgctgtt
     6661 ttatgtacta gctagtgcag ccaactttat ctatagtatt ttgttcacac aagtagtagg
     6721 gaagtcaacg aaccgaatgc gtatcggtct attcaacaaa ttggaaaaat tgacgatccg
     6781 tttctttgat tctcatcaag acggtgaaat tttaagtcgt tttaccagtg acttagataa
     6841 tattcaaaat agtttgaacc aagctttgct tcaagtcatc acgaatgctg ttttacttgt
     6901 cgggatcttg atcatgatgt tccgccaaaa tgtagagtta gcttgggcta caattgcttc
     6961 cactccagta gccgttttga tagctgtctt gattatccgc aaagcacgga aatatgtgga
     7021 tcttcagcaa gatgaagtgg ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca
     7081 acgtgtcatc atcacaaatg gcttacaaga agaaacgatt gaaggattct tagagcataa
     7141 tcaaagtgtt cgcgaagcaa ccttcaaagg acaagcttat tctggtctac tatttccgat
     7201 gatgcaagga atgtcactgg tcaatacagc gatagttatc tttttcggcg gctggttagc
     7261 attaaatggt gatttggaac gttcggtggc attaggctta gttgtgacat tcgtccagta
     7321 ttcacaacaa tattaccaac cactgatgca gatttcatct ggatacagca tgatccaatt
     7381 agcgattaca ggggcaagac gattgaatga aatatttgat gaaaaagatg aagtcaaacc
     7441 agaaaacggt aaagcatttg atggtgttga aaaaggtctt gcgttgcagc acgtagattt
     7501 tggttattct ccagaaaaac tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga
     7561 aatggttgct cttgttggtc caacaggatc ggggaaaacg acgatcatga atctgctaaa
     7621 ccgtttttac gatgtcaatg atggttcagt agtcatcgat ggaatagata tccgagatta
     7681 tgatcttgat gcattacgta gacatgtagg aatcgttttg caagaatctg tattgttctc
     7741 cggaacgatc aaagacaata tcacattcgg caaaccggac gcttcagatg aagaagtcat
     7801 caatgcagca aaacaagcga atattcatga cttcatcatg acgttagaaa aaggttatga
     7861 tacagagata tccgaagaaa ataacttatt cagtacaggt caaaagcagt tgatcagtat
     7921 tgctcgaacg atcatcacaa atccatcttt attgattttg gatgaagcaa caagtaatgt
     7981 agacacggta acagaagcaa ggatccaaaa agcaatggat gaagcaatca aaggaagaac
     8041 cagctttgtg attgctcacc ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt
     8101 gcgtgatggg gaagtcattg aagaaggcag tcatcatgag ttattagcag aaaatggatt
     8161 ttatgcggaa ctttatcata atcaatttgt atttgaaagt taaattaaaa gaacctcgtt
     8221 ataacggggt tctttttata tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DAPBBG010000087         7917 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPBBG010000087 DAPBBG010000000
VERSION     DAPBBG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.091"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..715)
                     /locus_tag="RD477_002596"
     CDS             complement(152..715)
                     /locus_tag="RD477_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDT7634904.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(730..1773)
                     /locus_tag="RD477_002597"
     CDS             complement(730..1773)
                     /locus_tag="RD477_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634905.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDKKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMQTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1906..2310)
                     /locus_tag="RD477_002598"
     CDS             complement(1906..2310)
                     /locus_tag="RD477_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDT7634906.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2478..3113)
                     /locus_tag="RD477_002599"
     CDS             complement(2478..3113)
                     /locus_tag="RD477_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDT7634907.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLGSMIFGVLTRLE"
     gene            complement(3379..4431)
                     /locus_tag="RD477_002600"
     CDS             complement(3379..4431)
                     /locus_tag="RD477_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634908.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4444..5382)
                     /locus_tag="RD477_002601"
     CDS             complement(4444..5382)
                     /locus_tag="RD477_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDT7634909.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5833..6483)
                     /locus_tag="RD477_002602"
     CDS             complement(5833..6483)
                     /locus_tag="RD477_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634910.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6583..7092)
                     /locus_tag="RD477_002603"
                     /pseudo
     CDS             complement(6583..7092)
                     /locus_tag="RD477_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
     gene            complement(7455..7838)
                     /locus_tag="RD477_002604"
     CDS             complement(7455..7838)
                     /locus_tag="RD477_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7634911.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNKVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         2452 a         1635 c         1220 g         2610 t
ORIGIN      
        1 ttctagatgg tcattcatgt tagaagaatc aaactatttt tttactgtcg taataatatt
       61 aaaacactag aacaaatcaa ttaaattcca taaaatataa ataatagacc tagcatcatc
      121 tggtataaaa tggatgctag gtccgttata tttactcaaa attgattcta cgaatagatc
      181 ttcttatcat ttctatttta gtagtcatgc gttgttgttc ttctaaaggt ggtaatggta
      241 taagtagttt agataaattt gtaccattta catttggttg cccagttccg taagaaattt
      301 tttctaataa cttccaatat aatggcgagt ctaagaagca gtctacgtat tctgataata
      361 atttctcaac taacctaatt cgaatcaagt aagaagcaaa aacagattcc tcacttattt
      421 ccttaaccaa gaaacttttt cccatagttc ctcctgtcct tgctatcaaa atgtcatttt
      481 cttccaatct taaatcaact agtttagaat tactaatatc acagtaaggc acactggacc
      541 aatttacacg tccctcttgt atatctgtga ttctcaaaaa cttaacattg ccttgttttt
      601 tcgccgaatc tgtatagcca tattgaatct tagtagaaat acttcctaaa taaacccata
      661 gtcgaacatc gctagatgat ttttcttgtc gttctttatg aacaacagtg atcatcttga
      721 gttgtccgac tattttttag ttgcagcaat ctttttggga cgcttatttg catgaagcaa
      781 cgttgctaag tctagtaata aaacattggc taacacagga ttggctttct cgcgatacgc
      841 tgcggcgcca ctttcaaaca atactgctag acgatcttct acaacaacga tttgttccaa
      901 atacggtgga aatttgattc gttttaggat ccggctagga gagtcccgct cggacactaa
      961 gagttccgag gtataaggac cataagaacg actggctaag agataatggc cataaaaagc
     1021 cacaccttga atcttatcag atgtttttct gctcattgtc acggttggtt ggattttgtc
     1081 tttgcggcga attgctgttt gcatttcagc tatcgtcggc aatagataga aagtcaactg
     1141 tccttcacct tttagtgtga agttgccaga taccagttga tcattgtttt gagtcaaata
     1201 cgatgcttgt ggaatctctg agagatcagt tgtcagccaa taagcgattg gctgtcccgt
     1261 ctcttcagat gtatctgcta agatatcgat caaacgtaaa gcggctactc gtccagtagc
     1321 agatcctgtg attgtcaacc ataaaagttc ccgtttttta tcatatacta agccccctac
     1381 atgtggccgt ttaggtaaaa tcagcagttt gatcggctgt cctgtttctc tatccagtac
     1441 atgaataata gaatgatgct gatggtcata gcagtaggca gaaataaaca caaaatcttg
     1501 agaaaaagcc aacccttgcg gcgtcatcca ttgacaaacc tcattttcgc cggtttccgg
     1561 caccaccatt cttgtttctg ttaatccagg aatgttatat tgttttgctt cagccttttc
     1621 ccaagctgct tgaacaatag gataccgttt taactttttt tcaatatcct gatgtatccg
     1681 ttgctgcttc caatgcttaa actttaggac atagtaaaca aggaggccaa tgaccagcaa
     1741 cactaaaatc attattgcta cccgctgtgt catttttttg cctccttgtt ctattcgatc
     1801 aatcgtacat ataccgctat taattaccgt aactacatgt ttcttttact agcgtttctt
     1861 tataggatct ttcataatct catttatagt aacattaaga atcccttatt ctactatagt
     1921 cacttccaca tcaatattgc ctcttgttgc tttagagtat gggcaaaatg cgtgggcttc
     1981 ttctgcgatt ttctctgctt ggtccttctc aagtcctggc aaagaaactt ctaaaacaac
     2041 cccgatttgg tagcccccat cttctaattg aaataatgat acccgtgctt tgacttcaga
     2101 atcagactca agattatttt cttccagcat gtgttgaacc gcgccgttga agcaagatgc
     2161 gtatccagcc gcaaaaagtt gttccggatt tgtcccttca ctatggattc ctgggcggtc
     2221 gatcttcatg tgcatacttc cgtttggtgc ctcaacctcg ccatcacggc ctccacgatt
     2281 gatcattgct gtttcgtaca tttttttcat tgagatcatc ctttctttcg tcgttctata
     2341 ttaagtgtaa cgagtatttt ttaatctgac aaaggtaatg cttgcattcg gcgacatcag
     2401 ttagacaaca tcgatgatca gtgatagaag cagtttatct atacaaaaaa agaagggttc
     2461 tatgatattt ggtgttgtta ctcaaggcga gtaaggacac caaatatcat agaacccaaa
     2521 tagttaaaga aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga
     2581 ctaatcgacg gaatccgtgc taatccgtaa agacctgcaa ttagtaaact aataactgtt
     2641 aaaacaagat aaaaatggct aagcgtacct tgaccacttt gacgataaag cagaacaagt
     2701 aacaatggga aaagtagaaa gcctgcatag cctagcccag aacctatatt atggatccaa
     2761 gtcgatagat tccaagagct ttcattcgta ttgatactga ataacccagt aaaaatacaa
     2821 tcacctatcc catataaacc aactgttgca acagcaagta ttgcaagtgt ttttgatgtt
     2881 ccaacaaata ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact
     2941 gaccagacca agaaagcacg tctgactgga ctatctacgt caccaaacac actaattacg
     3001 gtagtcattt gattcagttt gggataaaaa aggccaagga tatacggtgt cgataaatca
     3061 ctaatcacac ctaataataa aaaataaaaa ccatattttt gcaaaaaaat cattttcttc
     3121 ccctactttt tagtgattat tcgatacatc caacaatcaa attttttcac attttaacac
     3181 atatttttat tctagttgcc ttttgaacaa tcaataacat aaaggtgagt ttcttctaca
     3241 ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc ctactcaaaa
     3301 aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca ttgattgaaa
     3361 atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat catacagttt
     3421 aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga tgactaacca
     3481 acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg gaccaacaag
     3541 tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata gagcgacagg
     3601 aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt ttctaaatag
     3661 attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac ctgacttatt
     3721 aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca tcgcagccgc
     3781 accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg aaataggaga
     3841 gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg gttgtaaggt
     3901 agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa aagggaataa
     3961 ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca caatcgacac
     4021 tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat
     4081 cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac ccaaatcagg
     4141 tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca tcattctcat
     4201 cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg ttgctagttg
     4261 aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa aatattttaa
     4321 aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg ctgtaccagc
     4381 gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca ttcaatctct
     4441 cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca agaaatcaca
     4501 ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat tgatataatc
     4561 gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat aatgtaaccc
     4621 aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga catctagttc
     4681 aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca ccattttatg
     4741 agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc atagtccgtt
     4801 cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt cagaaaaatg
     4861 agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg cttcttttcc
     4921 atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct gaccaggacc
     4981 ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg gtacaaattt
     5041 ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg tatctttttt
     5101 gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg taaatagaat
     5161 aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg gtagttttgc
     5221 ttctacctct ttaccattaa aatttgcttg taatccatgt tctttgattt gttggacatg
     5281 ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca ttaacccaaa
     5341 gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt cctccttttt
     5401 ctttctatta tacttatttg agaatcattt cacaaccttg ttttgttatt cttctcttat
     5461 ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa aaactggaat
     5521 agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag aaacattcat
     5581 agaaaaaacc tcactcctct gattataaaa gaggggacat taacctcgtg tatactaaaa
     5641 ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt gtagtacgat
     5701 gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac aatacactta
     5761 tttaaaaact tctttcccag attttcttcc atttttacag ttataactag tattatcatg
     5821 aaaaatagcc ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat
     5881 tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat
     5941 ccaagcccag tccaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc
     6001 atattccgtc cttaaaatta tattttcagt gccagacaag tgatttgtat tgagttgata
     6061 aaaaagatct ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat
     6121 tccatcagct ctatgtgtta catacaaata attgtatgac ctcacttcac ctaatgaatc
     6181 aattatttct tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt
     6241 taacaaaagt tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct
     6301 actactaatc caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt
     6361 cgctaaatac tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct
     6421 acatcgattc tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt
     6481 cataaaacca atccagctcc tcgattaaga taatatttta atctcataca aaaactagct
     6541 ctcatcaaaa caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt
     6601 ataaattcct aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt
     6661 tcgttggcct tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc
     6721 ctcagcaaaa acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt
     6781 tagcaacaca ttgttgatat atttgatttc cttgcttttt tacggcgtgt ctactatcgg
     6841 cttatctttt tgcggaccac gtgccaagag aatcaggcca tttaggaatg cgtcaaaaac
     6901 ttggttgttg atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg
     6961 tactttggtc agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa
     7021 agctttaatc aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc
     7081 attgttattc atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc
     7141 ttgctaagat ttttaggatc aatgataaaa accgcatcac ttatgatacg gagctgggta
     7201 tcatgagtga atgatattta taaatcactg gatattgaag tatattctgt tttctcaact
     7261 tatttagtca gattttttaa tgaagatttt ttgagtaaaa tttctataat tcctacttat
     7321 tgaaaagtag tttgtagtgc tgcttctacc tctctcatct gttctacaaa ttccaaataa
     7381 cttgacgcag cttgcggaat gaaaaaatga tatgtgaata tttctcctcc tagcccgctt
     7441 aatatgccta ttttttatac ccatgatttg caggaagggg atcggaagta ggttgcagaa
     7501 agccgccttt cataatctgt cggaaaccgg acatcaaaac ggattatttt acttgtcaca
     7561 tttgtatttt cttgtgagga agtgccaaat accttattta cttgagtgac attctcattg
     7621 tcctgtctga tattgcttcg tataaaagta ccagatccac gtctcgtata taacaaacct
     7681 tcatcaataa ggagttgtat agctttgcgg atagtaacac ggcttgtttt aaactctcta
     7741 gccagacttt cttgctcagg taatttagaa ccttcctgat attttccctc aacgatcctc
     7801 tcttttagag ccaaggctat ttctttatat tttggcatat tttcccctca tttcattgat
     7861 cctacaaaaa aattagtata tatacaaatt tttgtaaagt atatatacta ataaata
//
LOCUS       DAPBBG010000088         7822 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPBBG010000088 DAPBBG010000000
VERSION     DAPBBG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.092"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1182)
                     /locus_tag="RD477_002605"
     CDS             complement(<1..1182)
                     /locus_tag="RD477_002605"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7634912.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
     gene            complement(1361..2134)
                     /locus_tag="RD477_002606"
                     /pseudo
     CDS             complement(1361..2134)
                     /locus_tag="RD477_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2155..3357
                     /locus_tag="RD477_002607"
     CDS             2155..3357
                     /locus_tag="RD477_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDT7634913.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            4090..5058
                     /locus_tag="RD477_002608"
     CDS             4090..5058
                     /locus_tag="RD477_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7634914.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            5069..5884
                     /locus_tag="RD477_002609"
     CDS             5069..5884
                     /locus_tag="RD477_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDT7634915.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5952..6662
                     /locus_tag="RD477_002610"
     CDS             5952..6662
                     /locus_tag="RD477_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7634916.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6698..>7822
                     /locus_tag="RD477_002611"
     CDS             6698..>7822
                     /locus_tag="RD477_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDT7634917.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         2597 a         1238 c         1589 g         2398 t
ORIGIN      
        1 atggagattt tccagtcgtg cattcgaata cttcttcagt aatgccagcc gaatcccttt
       61 ctcgtatttg aaaaaggctt ttttgatatg gtgcagttcc aatggaactt ctccaggcat
      121 tgctcgaagc agctggaaaa aagtatctgg atctttttcc tgatacgcag taagcaggtc
      181 ttggtagtaa cgataagctg ttttaagttc aggagaaatc tttagaaggc ggtcaactac
      241 ttctgtttca gtcaatagtc tccaattgaa attgcgtctt ttcgttagtg tattgtaatc
      301 taaacgatcc tctgatttta aaagtaattt gaataaggat ttcaattggc gatactgttt
      361 agcttgtatt gggtcttttt gatcaagtgt tttcatcaca cgttttcgaa ccgcatcgaa
      421 gaagcggttc agatgttgaa caatgtgaaa ccgatcgacg atcacttgtg catttggaaa
      481 acactcttta actagtgaga aataaggagc gttcatatcc gttacgatgt aatgtacacc
      541 caacctcgct ttgagagaaa aacgcatgaa aaaagcaacg agatcttttt tacggcggct
      601 aggtaagatc tcaaacagtt tcccggtctc accgtccata caagaaaaag aaaatttacc
      661 gtgccatgta gaaagagaac gaaactcatc gaataataga caccgaggta aatactcaag
      721 attctgacgg atatctgatg acaaggcgga caatgtccgc tgaacagttg tcacagaaac
      781 atcgcaatgt ttaccaattg cttttataga ttgacgctct gtcaattctt ctaagatctt
      841 gtaatgaatc tgcttagaaa taaagcagaa tggggtaacg agatacgtcc gagcggtaaa
      901 atatttttta catgctcggc agaggtatcg ctgtttcttc aaccgaagat aggtccgttt
      961 attaccgcta cgattcagga gaatcataga gatcttcttg ccatttttaa cgatttgctt
     1021 tgtagaatct ttacaacacg gacaacgtct gtaggtacgg atcagagttc cctcaataac
     1081 gatacttttt ctgcttttat aaaaagtttc ttcaaaggat tgaatttgga tatttgagtc
     1141 tttaatagta aagagttgtt tgataaaatg attcatggac atacaagaat tcccccagaa
     1201 tgatgttgta ggagactcta ttctaccgga aattttgtat gtctttttgt ttttttacaa
     1261 aaaaactagg acagatagaa aatttccatc agtcctagaa attatagagc caataaaact
     1321 tagtccgata tgcggcgcgt tctgtgcgcc gcatattttt ttagttttca aagatcagac
     1381 cttgttgtaa atagatccgt gcccgaaagt tataaaagtt gcgataacca aacgcaattc
     1441 gtttaatcag cttgatcttg ttatacagcc cctcagtgac gccgtttgag tgggacacct
     1501 taaaggcatt tgaaataccc gattggtagc gatggaagat cgtgaatttc tttttaaacg
     1561 actgaggtaa cgtagatgaa attgttttaa gggtttcttg aaagcctacc gtatctcgtt
     1621 taacgaaata atagcgtaat aattgacaaa aatgatacgc agttcgcaat tcttcgttgt
     1681 agcttaacag ctcattaacg atttctgttt gactaattgg tcgtttaaac agtcgatgat
     1741 aatgccgttt gagtgcctcc catgtcgtgg aatctttgag taagagtttc caatatcgct
     1801 tgagccgacg gtacttcttc tgattttcag agtgtgaggt tcgaaaacga ttcatgatct
     1861 ggacacgtaa ttgattgaag ctgcgattga tgtgttggac aatatgaaag cggtcagtta
     1921 cgatctccgc atttggaaag accgttttaa gcaactgaca atagctggca ttcatgtcca
     1981 tcactaggta cttgaccttt aaacgggcct ttctaggaaa gcgcaggaag tattgagtta
     2041 gtttgtaaag tctgcgatct tcaagggtcg taaatagctg attggttgtc ccatcaacac
     2101 aaataaaact catggaacca gaagtggcaa cattgtgtac ttttctttgc aatcttgtgt
     2161 acaaaactat tgcaaaacac agcgcataag aatccttaca aaacagtaga tgaagcaacc
     2221 caattagatc ttgttatagt tgttaatcag ctcttaacgg gttacgattc aaaatgggtc
     2281 aacaccctat acttagataa gtcacttgaa tatcagaaca taattcaggc tttttcacgc
     2341 acaaatcgtc ttaatggtcc tgaaaaaccg ttcggaacca ttcgttatta tcgtaaagca
     2401 catacgatgg aacagaacat tgaagaggct attgagttat actcaggcaa acaagcaaaa
     2461 gggttatttg ttgaaaaact atataataac ttagaaaagt tgaacgatat attcgaagaa
     2521 attaagtccg tatttaatat ttctggcata gaaaacttca gtcatttacc ggaggttcca
     2581 ggggaaatta agcaatttgt aaatctttat aatcaactaa tccattgtta tgaatcagcg
     2641 atgatacagg gattcacatg ggcaaaatcg acttatgaaa tccaggatta tgaacctata
     2701 acagtagtaa tcacagaata tgaatttaat atcttggcac aacgttacag cgagatagag
     2761 agagaaccta gagacggaaa tgatagtata gcgtatgaca ttgattacca attacctgaa
     2821 ggtagtcgta gtcgtattaa ttatgagtat ttaaacacaa acttcaaaaa atacatgaat
     2881 ataaaagaag aacgtgattc gaatttaatt caatcattgt taaacgactt acatcgcaca
     2941 tttgcgcaat taccagtgga gttacagcca tatgctgaga tgattctaaa tgatgtaaat
     3001 aatggggaat tagttatcaa agaaggatgg gaatttactg attacctcaa tgaatatcag
     3061 cttagtgaag aggacgattt tatccaagat ttagtggatt caaccaatat taatgaggca
     3121 ttattacgtg agatgttgga tttacgctta accgaggtaa acattaatga atatagtcga
     3181 tttgataaat taaaatcaag tgttaacgta ggtcatttca gtggatatat cgagaaaaat
     3241 ttaggtaaaa tggtaatacc aatcaaggta aatatgctaa ttgacagatt attgcgtgca
     3301 tttttattag aagatgtatt cgatgttaca gagtacataa agaattactt taactagagt
     3361 acacctacat cttatagtac ataaaaaaat tcatcttttt tagtttaata attcaccaaa
     3421 aaggtgagta atgccatttt tgaaaattga gcttgtttta tgtctacatc tgtatccgta
     3481 tataatccaa aggattataa actcagaaga gtctttattt tttctgaata taactactgt
     3541 atcaaaaaca atcgattatg agacaaaaca aaatgacctc ttttatttaa gaaaggaggt
     3601 cattttttgt atcataaatt gtattaaaat caatgtatca ctttttaatc aattttaaga
     3661 ccataaattg cgtactaaaa gaaattctgc aattcaaaat aaagcatttt aagccatttt
     3721 caaatttaag tgcacccatc tacccgtcaa atcactttat tttccactcc agtagtttcc
     3781 taattcaact gtgtgcaaaa cgatttcttc taaacaaatc aagacaaact tataaacctt
     3841 catcccagca ccataattat ttaaatcata ggcatttttc gaaattagta tatgtactca
     3901 agtttcactg cagattattt atatttttta gagaaataaa tttttcctat tgacaaacac
     3961 aaagaaagcg gatacaatac aattatgtca catgacgtta tatgacgtta tgtaatatct
     4021 ttacatttaa tcagttactt ttattttgga taaaattaac atttttaaaa ttttaaggag
     4081 gaaatgttga tggatgtagc aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag
     4141 atttctggaa tttatttggt ggcttgtggc ggttcgctgg tagatatgta tgtctcagat
     4201 tattttctta aagccgaagc atcgaagtgt attacaggct tatatacagc gaacgaattt
     4261 aaccatgtaa ccccaaagca attagatgat aaatctgttg tcattctatg ttctcatgga
     4321 ggaaatacag cagaaacggt aaaagcagga gaagtcgctc aagctagagg agctgtgact
     4381 gttggtctga ctcataataa aactgcggat ttattaaagg tttctgatca tagtttcctt
     4441 tacgaatggg gagatgatgc aaaaatagtc aataatccta tggccatcat tcttgattta
     4501 actgtatctt tggtcaatgt taccgaaaca tttgaacatt acggtaaatt cagagaaggc
     4561 atgaaggtta tcgataccgt aattgaaaat ggcattaagc aggtgcagga tcgtattaaa
     4621 gtgtttgctg ataagtacaa agatgaaaca atgttttata tcttaggtag tggaccatca
     4681 tatggacatg cttatggttt ttcaatttgc tctttgatgg aaatgcaatg gcttgattca
     4741 acttctgttc attctggaga attcttccat ggaccttttg aagtaacaga caaagatacg
     4801 aattttattc tcttgatgag cattggacgc actcgtgctt tagatgaacg ggcaaaacga
     4861 ttccttgata aatatgcaga aagagtagaa atcgttgatg caaaggaact aggattggat
     4921 atgatatcag atgaagtttc tgaattcttc aatcccattt tattctacag tattctttca
     4981 gaatatcgtt cagctttagc agatgcacgc aatcatgatt tagatgttcg tcgctatatg
     5041 gggaaagtgg agtactagga ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt
     5101 cgataaatat gaaaatctgg gaattatgta tcctggagga aattcattga attttgcagt
     5161 ttttgcaaaa aaactgggac atgaaagtag ctttatggga gtttttggct cagatgagga
     5221 ggcagctcat gttttatcag caataaaaaa aataggactg gacaacagtc actctcgctt
     5281 tgaaaaaggc gagaatggct gtgccagagt gaagattgat gagggcgacc ggattttcct
     5341 tggcagcaat tcgggaggtg ttactcgtga atacccgatt cagcttactg aagaagacag
     5401 agaatattta aaccagtttg aattgattca tatgggatta tatagccatg tcaatcatct
     5461 attagaagag ttacaaaatg tttctggaaa gatttcttat gatttttctg acgactttac
     5521 ggaagaagag gttcagcggg caattgataa agttgatttt ggcttctttt ctatagaaga
     5581 cttttccgat gaagaagtaa agcagttttt gatgaagcat ttttgtgagc gaaatgatgt
     5641 attgattgca acccgaggag aaaaaacggc tattggttat gatggagcaa agttttatga
     5701 agtgaacccc gtgctagaag aaccagtcga tacaatggca gcgggagatt ctttcttaac
     5761 agctttccta ctttattttc tcgaagggga cgatatagtc accgcgatga aaaaggggaa
     5821 tgaatttgct gggaagtctt gtctggttga cggttcgttc ggctttggta ttcattacga
     5881 ttgagcttgt tttaaattcc tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg
     5941 aggttaaaaa aatgctggat acaaatgcac aattaccatt gtacttacaa ttaaaagaaa
     6001 tattaaaaaa taaaatcaat acaggggagt atcccgagaa tacgaaaatt cccgctgaat
     6061 cagagctgat agaaagttat gaggtcagcc gaatcacagt gagaaaagca gtagaagaac
     6121 tggtagaaga agggtaccta cttaaaaaac aagggaaagg aacttttgtc aaccggcata
     6181 aagtttttcg taaaattgaa tatgttatgg gtttcagtga gagttgtaaa tctaatggtt
     6241 ttacaccgac tagcattgtt ttggaacgca agatcattcc agcaactgaa gggatggcaa
     6301 aaaaactcga tatcgctcaa ggtgatgatg ttttatatat ccagagaaag cgaatggcgg
     6361 ataacatccc cattatgtta gaaaacaatt atttttctta tgaaagatat agtttcttaa
     6421 tggaggaaga gctgactgat tctttgtatg aagtacttaa agtccaaggt accttagcca
     6481 tcaatcctgg ggaaactgca cttgaaattg tcacagctga tgaccaaatt gccaaaataa
     6541 tggaagtagc tgttgggacg ccctttttct ttatggatac tttaatcaac gatcaaaaca
     6601 acataccaat tcatgtaggt aatcaatatt acctgggaga agtatatcgt ttttacttat
     6661 gatttcctgt tttgatttca aggagtagat tttatttatg aagcaaatat tgcggtcaaa
     6721 aatgattttt gatggagagc agccaccttt caaaggttat attgtaatca atgaagggat
     6781 cattgaaaag gtggaaaaca actggaatta cagtgatcta ttaaatgaaa acacggaatt
     6841 aattgcttat gatgatcaat tcgttatgcc agcaattcat gataatcatg tatttttcag
     6901 tggttatttg gctatgaatg ctggcataga tttaagcgat acatcttcag ctaacgaggc
     6961 tgtagagcgt attaaagctg taatgaacca taaaaaagca gatgaaccaa tttatgctca
     7021 tggctgggat gctgccctgt ggaaaactga cccatcagaa gagctattga atgaaataaa
     7081 ccactttggt ccaatcacag cgatcgacaa aaatcgcagc cattgttgga tgaatgctat
     7141 tgctaagcag acttatggtt ttacagaaaa agaaaccagt gctgaaagtc gagtattatt
     7201 aattaaggaa atgctagcaa acaaaaagct tttagcagag gtttacgctg aatttgaagc
     7261 attactgctt tcaaaaggtg ttatatcaat gaaagaaatc gtttttgatg acgcagaatt
     7321 tctttctaat atacctcaga ggaaaatact tagtaaccta tatgtgcagg cagtccaaga
     7381 acctctacag catgaacttc tatatgagta taaggaaaaa gttttccctg aaaaggtccg
     7441 ctttggtgga gtgaagatca tggtagatgg tgttgttgcg gatagaagtg gagatattta
     7501 cggtgtttat cagtcaggag tagcttcacc cgagattgac tatgaggcaa ttaaagcaga
     7561 agttgctgca ttcaatgaag tagagattcc ttgctgtttg acaacggaag gagataaagc
     7621 gggttataaa gctgcgtcaa ttcttgtcga atttggaaag cggttacctg aaggcgtttt
     7681 taattcgatt agtgatttgg aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg
     7741 acaagtagta gcagaggtat atccacaaat tctcggcttg aatcaaacgt tagaagaggc
     7801 ttacatgtca actgttataa aa
//
LOCUS       DAPBBG010000089         7687 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPBBG010000089 DAPBBG010000000
VERSION     DAPBBG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.093"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            110..487
                     /locus_tag="RD477_002612"
     CDS             110..487
                     /locus_tag="RD477_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7634918.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            475..813
                     /locus_tag="RD477_002613"
     CDS             475..813
                     /locus_tag="RD477_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7634919.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            947..1390
                     /locus_tag="RD477_002614"
     CDS             947..1390
                     /locus_tag="RD477_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDT7634920.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1652..2467
                     /locus_tag="RD477_002615"
     CDS             1652..2467
                     /locus_tag="RD477_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7634921.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2477..3139
                     /locus_tag="RD477_002616"
     CDS             2477..3139
                     /locus_tag="RD477_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7634922.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3345..5009
                     /gene="alsS"
                     /locus_tag="RD477_002617"
     CDS             3345..5009
                     /gene="alsS"
                     /locus_tag="RD477_002617"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDT7634923.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5022..5732
                     /gene="budA"
                     /locus_tag="RD477_002618"
     CDS             5022..5732
                     /gene="budA"
                     /locus_tag="RD477_002618"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDT7634924.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMGFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5862..6359
                     /locus_tag="RD477_002619"
     CDS             5862..6359
                     /locus_tag="RD477_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDT7634925.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            6544..6804
                     /locus_tag="RD477_002620"
     CDS             6544..6804
                     /locus_tag="RD477_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634926.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(7166..7405)
                     /locus_tag="RD477_002621"
     CDS             complement(7166..7405)
                     /locus_tag="RD477_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634927.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         2549 a         1181 c         1597 g         2360 t
ORIGIN      
        1 ccagcttagt tttaatagaa atgagtattg acttggagta cacttaaagt tgtagaataa
       61 ataaaataca taagtaaagt ggcaagaaat taagagaaag agtgatcaga tgaatatcaa
      121 acaagtcagt gaagaaaagg gcatttcggc cgatacattg cgctattatg aacgaatcgg
      181 gttgattcca ccagttaatc ggacaaaagg cggaatccgt gattatacag aagaagacct
      241 aaaatgggtc gactttacga tctgtatgcg aaatgccgga ttatcgatcg agtctttgat
      301 tgaatatatt cgcctatcta gcgaaggaga agcaactgta tttgagcggc gtaagctttt
      361 gattgaagaa agtgaacttt tgacgaaaaa aattgctgaa atgcaagaat gtttggaacg
      421 tttacaagga aaaatcaaga aatatgatcg tgtgttatct agaaataaaa tggaatgtat
      481 caagtaaaaa aatcaaggag catcgctatg gagttacgct tactaaaata tttttggaca
      541 attgcagaag aaggaacaat ctctcaagcc gcagaagttc tacatcttac acaaccaacc
      601 ttaagccgac aactgaagga gttagaagag gaactaggaa cagaactgtt tgtacgcgaa
      661 aaccgcaaaa tgatcttgac agaagcaggt tattttctaa aaaagccgag cagaagaaat
      721 tttagattta acgaatcaaa ccactcaaga attcgagaac agaaaaaaac aattattcag
      781 cggccgattt acgatcggct gtgtggaagc tgataattcg aatacattgg cactattact
      841 agaaaagttg atccgtgatt atcccagcgt tcaatttcat atccacagcg gaacaagtga
      901 tgatatcacg gagtgactag aaaaaagcct gttagattta gcgattttgc tagatccagt
      961 agatacagag aaatacgaaa cgattttgtt accaagaaag gaaaaatggg gattgctcat
     1021 tttagaaaat tctgttttag caaaaaaaga cgtgataaca aaagaagact ttgcgaatgt
     1081 acctcttttg atctcaaagc gtccagaaat acagcgaatg tttgctgact ggttaggcat
     1141 aaaaaaggag cagttgctga tcacaggaac gtacaatttg attttcaatg tatttgcgct
     1201 aattgagaat caggtaggtg ctgctttagt catagaaggt gctgtaagtc atctttcaaa
     1261 gcgagtgaca ttcgttccgc ttgacccaat gattgaaacg aactgcatgc tagtctggaa
     1321 aaagaatcgg attttatcac ctgtagtcaa tgaattgatt caccgtttca aagaagcttt
     1381 tgaacagtaa taagtatctc taatcgaaga aatttcaata caaaagcgag acgtatgaca
     1441 gtcttgcttt tgtatttttt catttttaaa tcaaaaaaaa taaagagatt tacataactt
     1501 gttaaataag cgcttacaaa cataagaaga acggaaatga atcagctata ataaccgttg
     1561 ttctggattg taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga
     1621 aagaaaaaaa gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat
     1681 tctgctgatt gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc
     1741 attcaaagca aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca
     1801 tttagagaat tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac
     1861 agctttttta agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta
     1921 cgggttttct aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac
     1981 gattgctgtc ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg
     2041 tttaacatat acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt
     2101 cttgttgaag caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga
     2161 tggcttatca gagtgggaaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat
     2221 tgccagtttg acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat
     2281 ttatttgtct tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct
     2341 atttgatgca gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc
     2401 ttttatattt ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa
     2461 ggaataggag gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt
     2521 ttattggttg tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta
     2581 tatagcagct gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc
     2641 tttacttttt attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa
     2701 agaaaaaagt ttttcaccag tacgtttgtt cataaagacg tttcgccaat tttggctaaa
     2761 gggatttttt tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt
     2821 cttttttata cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg
     2881 tttatcagtt tcctttagca tcaactgctg gtattttcag gtgagaaatc cagcgtcgaa
     2941 accaaatcaa gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt
     3001 actagatttt ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg
     3061 tattctacta ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat
     3121 gagaactatg gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt
     3181 tatgtaaaaa ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa
     3241 aacgtgctat acttttgaat gaaacaggtt tagaaactgt atcacttatt tgtgattatt
     3301 tctaactaat aattaaaaat aaagcataaa aaggacgatg ttcaatggcc gaaaaaatga
     3361 aacaaggttc gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg
     3421 gtataccagg tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat
     3481 taatcgtgac tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta
     3541 caggagagcc aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag
     3601 gtttggtaac agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac
     3661 gaagcgatct attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa
     3721 tcacaaaata cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg
     3781 cttatcgtat ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg
     3841 tagtggatgc acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt
     3901 ctgcttctgc agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg
     3961 tactattagt aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag
     4021 ttgaaaagac agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg
     4081 aattagaagc gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc
     4141 ttttgaaacg aagtgatcta gttattgcaa ttggctatga tccaatcgaa tatgaagcaa
     4201 gaaactggaa tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa
     4261 tcgatccttt tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt
     4321 tattgacagg ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat
     4381 ctcttcaagc aaaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct
     4441 tgcatccttt agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta
     4501 ccgtagatgt aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc
     4561 gtcatttgtt attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt
     4621 ctgctgcatt ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt
     4681 tattctctgc ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga
     4741 tttggaatga tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg
     4801 cgtcaggtgt agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta
     4861 aaggaatccg agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga
     4921 cagaaggacc cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag
     4981 aaacaatctt accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat
     5041 accagcacgg gacattagga gctttgatgg ccggcttgat ggatggaaca gaaacgattg
     5101 ctcatatatt agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag
     5161 tcatcttttt agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag
     5221 atggaagtga acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt
     5281 ctgtctcaaa aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg
     5341 gggaaaacct atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta
     5401 tcatgccgaa gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag
     5461 aattccaaca aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc
     5521 aaggagttgc tgcagcggga tttcacctgc attttatcga tgatacacat acatttggcg
     5581 gacatgtaat gggttttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc
     5641 ttgtacagca ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg
     5701 atttagcagc agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg
     5761 attaacctga cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag
     5821 ctataatctt ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga
     5881 aaaaagatgc atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata
     5941 actggttagc aaatttagca aattcatctg cattgttgaa tgaaactttg cctgagactg
     6001 tctttgcagg atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc
     6061 gagtttcttg cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac
     6121 aagaaacatt gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag
     6181 ccgcacaatc agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag
     6241 atatcgattg cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat
     6301 atgtagatct tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa
     6361 atcgtctatt gagacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg
     6421 ggcatttctg tttttcgttt ttttattttt ttataaaatc acaagtgtta ttggctttca
     6481 taatcatgtt ttaatggtat tatcatttta aataatgaaa aacacattaa gggtggatga
     6541 aatatgaata acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg
     6601 aaacaaaaag tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta
     6661 gacgaatatc atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca
     6721 aaatatactc acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa
     6781 tatgaacata ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc
     6841 aagcttttga aggcacttta tttagttggc agtgattgtt catgattttt gatttattca
     6901 aaaggttcgc ctagagaatc tgtttctgta acgaggcctg gtggagaaac tcgataaaaa
     6961 cctatactgc cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca
     7021 tccgatccaa tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg
     7081 ttatcgtaaa gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca
     7141 ttatttgctg aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg
     7201 ctgacagttt ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta
     7261 ccggagaatt tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat
     7321 ctgaaggtat gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata
     7381 aactgataaa cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac
     7441 acctatgctc agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat
     7501 tagtcttagt aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt
     7561 taccatctgt attaacgatt atgcttctaa acgctttttt tatgaaaaaa tttataagtg
     7621 aaaacgtagg tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg
     7681 aggatag
//
LOCUS       DAPBBG010000090         7599 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPBBG010000090 DAPBBG010000000
VERSION     DAPBBG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.094"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(942..1355)
                     /locus_tag="RD477_002622"
     CDS             complement(942..1355)
                     /locus_tag="RD477_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDT7634928.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="RD477_002623"
     CDS             complement(1811..2296)
                     /locus_tag="RD477_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634929.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="RD477_002624"
     CDS             complement(2435..2650)
                     /locus_tag="RD477_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDT7634930.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="RD477_002625"
     CDS             2874..3674
                     /locus_tag="RD477_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7634931.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="RD477_002626"
     CDS             3693..5561
                     /locus_tag="RD477_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDT7634932.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="RD477_002627"
     CDS             5554..6045
                     /locus_tag="RD477_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDT7634933.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="RD477_002628"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="RD477_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDT7634934.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="RD477_002629"
     CDS             6605..>7599
                     /locus_tag="RD477_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDT7634935.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1443 g         2235 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gttcccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAPBBG010000091         6802 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPBBG010000091 DAPBBG010000000
VERSION     DAPBBG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.095"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            139..1095
                     /locus_tag="RD477_002630"
     CDS             139..1095
                     /locus_tag="RD477_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7634936.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRELLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELKIDQILE"
     gene            1372..2070
                     /locus_tag="RD477_002631"
     CDS             1372..2070
                     /locus_tag="RD477_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDT7634937.1"
                     /translation="MEENKTLVEVTTDKILRYLKEKQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQISRMKSLTQKIETSFREGTQDHVQLDIDFHTTIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMATN
                     RRELQKIIGKKETN"
     gene            2220..3536
                     /locus_tag="RD477_002632"
     CDS             2220..3536
                     /locus_tag="RD477_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634938.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLVTGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIVVSILIYLPFVKVANRVENDVI"
     gene            3553..4968
                     /locus_tag="RD477_002633"
     CDS             3553..4968
                     /locus_tag="RD477_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7634939.1"
                     /translation="MKITIPENFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAKIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYHSGNYGGR
                     IGCILNPEMVYARSSSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCRKQSIYF
                     DPATEDLETVQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEEQYMDEKGIVQDDYRIEF
                     IGQHLRYLIKAAEEGANCEGYMLRAFTDCVSPMNAFKNRYGLVRIELNDERSRILKKS
                     ASWYQSLIDKRELSLPDEAYK"
     gene            5281..5601
                     /locus_tag="RD477_002634"
     CDS             5281..5601
                     /locus_tag="RD477_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7634940.1"
                     /translation="MEKRTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQIESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            5626..5943
                     /locus_tag="RD477_002635"
     CDS             5626..5943
                     /locus_tag="RD477_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7634941.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LDA"
     gene            5972..>6802
                     /locus_tag="RD477_002636"
     CDS             5972..>6802
                     /locus_tag="RD477_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634942.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     V"
BASE COUNT         2197 a         1121 c         1484 g         2000 t
ORIGIN      
        1 aaaatacaag aaaaaatcat tttgactaac tccttttctt taaaatgtag taaactaaaa
       61 gcagaacgaa agaaagcgtt ttaaaaatgg taaggaggag aaaacttgta tattgcagat
      121 agtgcacgtt atgaaaaaat ggaatatcgt aaatccggtc attcaggttt gaaattaccg
      181 attttatctc tcggattatg gcaaaatttt ggtgattatg atcctatcca taaccaaaga
      241 gagattttaa gaggtgcatt cgacatggga attacccatt tcgaccttgc gaataattat
      301 ggaggccctg ctggagctgc agagaaaaac ttcgggcgta tttttagaga agattttcaa
      361 gcctaccgtg atgaactgat tatttctagc aaagcaggtt atcatatgtg ggaaggtcca
      421 tacggagaat ggggctctcg taaatctatc atttcaagtt gtgatcagag tctccaaaga
      481 atggggctag attatgtcga catcttctat catcatcgtc cagaccctga tacacctttg
      541 gaagaaacag cagaagcatt gatgcaactt gtaagacaag ggaaagcatt atatattggt
      601 atatccaact ataatggaga agataccaaa aaaatgactg aaattttaaa aagaaaagaa
      661 gcaccgttca ttattcatca gatgcgctac aatatgtttt ctcgtgaact actagaagac
      721 gacttatctc cagtattaga agaagaaggt ctaggagcaa tcacgttcag cccgcttgcc
      781 caaggtttgc tgacaaatcg ttatctgcat gggattcctg aagattctag agcacatcgt
      841 aaggaaatac cattcctttc agaagaacaa gttggatcaa ctctggaaaa aatcaaagca
      901 ctgcagacaa tcgccgtatc acgagggcaa tcattagcgc aaatggccct tgcatggaat
      961 ttgcgacaaa aatctgttac gagtgtactg gttggagcaa gtcggttaag tcagttacaa
     1021 gaaagtgttc gaatgatgga taatttagat ttctcaccag aagaagaatt gaagatcgac
     1081 caaattctag aatagaaacg atgtaatcaa actttttatc catctatcaa aaaatgtcat
     1141 caagaatcga atgaagctca tctattattg aacatcatac gttttgtcgt tcaaaataaa
     1201 aatgatcgaa ggctaggagt gaattttatc ctagtctttt tttatggtaa atagcttcgt
     1261 ttgtatatcc atgaacggat gaatcgattt ttatacaaaa aatcatcaga tgattcaaac
     1321 gtgttataat aattacgaaa aaatacatca gatgaaaagg aggtatgtgg tttggaagaa
     1381 aataaaaccc tagtagaagt aaccacggac aagatcctta gatatttaaa ggaaaagcag
     1441 ttgcaagtag gagataaatt gccgaatgaa tatgagttgt ccgattattt agaggtggga
     1501 cgcagcacgg tgcgagaagc tgtccgagcg cttgcttcta ggaatattct tgaagttcgg
     1561 caaggggcgg ggacatttgt cagtgataag cgagggattg ctaatgatcc actcggtttt
     1621 tctttgattg aagatcagga ccggatgata cgagacttgt ttgagctgag atatttactg
     1681 gagccaaaga tggcagggtt agcggcggaa tatgcaacga aaaatcaaat cagccgaatg
     1741 aaaagcttaa cgcaaaagat tgaaacgtct tttcgagaag gtacacaaga tcatgttcaa
     1801 ttagacatcg attttcatac gacgattgca gaagctagcg gaaacgtagc gttactgcat
     1861 atcattccga tcattaatga atcgattgct ttatttaaca aaaattatga tatccagcat
     1921 ttgaaaatcg agacgatcga tatccacaca gaaatcgttc aggctatcga gaggcggaat
     1981 gtacttgcgg catttgatgc gatgactgtc catatggcaa caaatcggag agaattacag
     2041 aaaataattg ggaagaaaga aactaattga cagttcatct gatgtatttt atgataaata
     2101 catcagatga atttttgttt tatatttggt aaaacggaca agcaaatagt ttttattcac
     2161 cgaaatgtaa gcgctgacta acaggcaaaa agagatgacg gcaaggagaa gaagcaggaa
     2221 tgaggggatc tggaatgagt tttatggata aattagccaa tgttttaggg aacgtggcaa
     2281 cgagaatcaa taatttaagg tatatcatgg tgataaaaaa cgcatttgct gcattgattc
     2341 cggtaattat tacgggtgct tttggcacgt tgttttcagc aatggtcttt gattcggaaa
     2401 atgggttagc acagataagt tttttgcgtt ttttagaaga attaaaacct atcgcatctg
     2461 ccatcagcta tgtcacacta agtttcttga ccatctatgc tgtttttctg atagggatcg
     2521 aattagcaaa gctgaataaa gtggatggtg tattccctgg tattatcgca gtgatgtctt
     2581 atttatctgt taatccaact gtttatgaat tagtgaatga aaatcagact gttatagtag
     2641 agaacgtatt ggctaaacaa tatacagata cgaaagggtt atttttggga atgttggtct
     2701 ccatcctttc cattgaactt tattgctggt tgaggaaaca gaaaaaacta cagctgagaa
     2761 tgcctgatac cgtgccaacg aatgtttctg ccagtttttc tgcgttattt cccacgattc
     2821 tgacagtatc aggtatcgcg gctgcggggt ttatcatcaa agaacttaca gggatgtacc
     2881 tttatgatat catttacaat gtcgtacaac gcccattaga aggtgtcgtt caagggctac
     2941 caggtatttt gttattgatg ttcattgcgc aagtcttttg ggtgattggt atccatggaa
     3001 atcaaatggt gaagccaatt cgagaaccgt tgcttttagc ggcaattgct gtaaatacgg
     3061 aagcttttga agctggaaaa gaagtaccaa acattatcac tatgccattt tgggatatgt
     3121 atatgagtat gggcgggtct ggggtcacga taggtctact gatagcagta ttgctagtgg
     3181 gaaagcgaga agatatgaaa caaatcacca agttatctct tgctcctggg atcttcaaca
     3241 tcaatgaacc agttattttt ggtatgccga tcatgctgaa tcctatttta gctatcccgt
     3301 ttatcttaac gccgcttgtt acaggaacga ttggttattt tgcaaccgct ttaggatttg
     3361 cggcaaaagc agttgtaatg gttccttggc cgatgccgcc gatcgtcaat gcttatctgg
     3421 ccacagcagg aaatatcggt gcagttatta cgcaacttgt ttgtattgtt gtttctattt
     3481 tgatctatct gccattcgta aaagtagcaa atcgtgtgga aaatgatgtg atctaaaaaa
     3541 caggaggaaa tcatgaaaat tactattcca gaaaatttta ttcttggtgc agcatcttct
     3601 gcttggcaaa cggaaggttg gaaaggaaaa aaagaaggac aggattcgtt tatagatagc
     3661 tggtataaaa atgaacattt tgtttggcat aatgggtatg gtccagccgt agcaactaat
     3721 tttatggagc agtaccaaca ggatgtagat ttgatgaaat ccatccatct gacacattat
     3781 cggacttcta tcaattgggc tcgttttttc actgattacg agaattgtat agtagatgaa
     3841 gaatatgctc agcatataag tgaaatgatc gactctttga tcgaagcaga cgtagaaccg
     3901 atgatttgtt tagaacacta cgaagtccct gcttttcttt tggaaaaata tgatggctgg
     3961 tcttcaagaa aagtggttga tttttatgct aagtacgcaa agattgcttt tgatcgttac
     4021 ggtgacaggg taaaaaactg gtttacattc aacgaaccaa tcgttcccca aacgcgttgc
     4081 tatttggatg ctgttcgctg gccgcatgaa caaaacacaa aaaaatggat gatgtggaat
     4141 tatcacaaag tattagcaac ggctaaagct gtagaaattt accatagtgg caattatggc
     4201 ggaaggattg gctgtatttt aaatcctgag atggtctatg ctcgttccag ttctccagaa
     4261 gaccaaaaag ctgcttatat gtatgatctg ttttataatc gagtattttt tgatccgatg
     4321 gtaaaagggt actacacaga agagttccta gagttgtgtc gaaaacaatc gatttatttc
     4381 gatcctgcaa cagaggattt agaaacggtt cagcatcata cagtagattt ccttgggatc
     4441 aatcaatatt accctaaacg cgttcgtgct cctcgttatc aatggaatga agagacacct
     4501 ttccatccag aacgatatta tgaagtcttt gatttacctg gaaagaaaat gaacaattct
     4561 agagggtggg agatctatcc aaaaatcatg tacgatatag ccatgtatct aaaagaaaat
     4621 tatcataata tcccttggct gataacagaa aatggtatgg gaagagaaca tgaagaacag
     4681 tacatggatg agaaaggaat cgtgcaagat gactatcgta tcgaatttat cggacagcat
     4741 ttgcgttatt tgatcaaagc agcagaagaa ggtgcaaatt gcgaaggtta tatgctacgg
     4801 gcattcactg actgtgtttc accaatgaat gcatttaaga atcgatatgg attagttcga
     4861 atcgaattga acgatgaacg gagcagaata ttgaaaaagt ctgcttcctg gtatcagtct
     4921 ctgattgata agagagaatt aagcttacct gatgaagcct ataaataact agaagttttt
     4981 cgaagatatt gattgtattt gatggttatt tggaataaag gaggttgtga aagtagcatt
     5041 ttgacctctt attcaaaaaa gttgtgacaa aagacttgtc acaacttttt ttgtgcacgt
     5101 taaaagaaat aaatacaata aaaaagaatt tgtgtgttta gtgtgcgctt tcttttttag
     5161 tgtgtatgca acttggtata gaccataaac gtgatattat caacttttta aagcgctttt
     5221 aaagttggca cactacttgc tttatataca agtgtaagaa tagaaaggaa cgtgtttaaa
     5281 atggaaaaga gaacaatcat gcttgtatgt tcagcgggaa tgagtacaag cctcctagtc
     5341 acaaaaatgc aaaaagcagc agaagcaaaa ggaatggaag cagatatctt tgctgtctct
     5401 gcctcagatg cagataatca aatagaatct aaaaatgtag acgttttact cttaggtccg
     5461 caagtacgtt ttatgcaaaa tcaatttgct gaaaagctag cacctaaagg cattcctcta
     5521 gatgtgatca atatggctga ctatggcatg atgaatggtg aaaaagtact cgaccaagca
     5581 gaaaaactaa taaacaaata ataaaaatat cggaggaaac aagaaatgga agatcaaaaa
     5641 aatctagaag ccatcatggg tctgatcatg tttggcggaa atgcaaaaag cgatgcaatg
     5701 gaagcgattg ctgctgcgaa aaaaggagat ttcactttag cagatgaaaa aatcaaagat
     5761 gcagaggaat ctttagtcca agcacatcac tctcaaacag agatgctgac gcaagaagca
     5821 caaggaaacc acgttgaagt aacacttttg acagttcaca gtcaagatca cctgatgaca
     5881 tctattgctt ttacagattt agcaaaagag atcattgatc tgtatcgtcg gttggacgcg
     5941 taattcacag taaagaaaag agggaagatt tatggataaa tttgttgcat ttatggagaa
     6001 acattttata cctgtcgcat caaaaattgg tgcacaacgg catttagttg cgattcggga
     6061 ttcatttatg gtgagtatgc cgttaatgat tttaggagca ttagcagtaa tgatcaataa
     6121 cttaccgatt cccggattcc aagaattgat gaattcaatt tttggcggtg aatcatggaa
     6181 aggattcggg gctgcagctt ggaacggcac atttgccatt ctttctgtct tgattgcttt
     6241 cttacttgcg tatcatttag ctaacggcta ccgaaaagac ggtgtttccg caggtgtgat
     6301 ttctttaggc tctttctttg ctttaggcgg agcattaggt atgagctcaa acggtttgtt
     6361 tattgctatt attgttggga ttatttcaac agaaatcttt gtacgtttaa gcggaaatga
     6421 aaaattgatc atcaaaatgc ctgatggtgt gccaccagca gttgcgaaag catttgcttc
     6481 attactgcca gcaatgatca ctatctctgc ctttgcttta gttgctgcaa tttttgctgc
     6541 atttggggtt gatgatatcg taggttcgtt ctatacagtg gtccaagaac catttatggg
     6601 acttgcgaac tcttatccat ctgcattatt gcttgcattt atcacaccat tcttatggtt
     6661 ctttggttta cacggggcga atatggtcga tcctttgatg caaacgatca atgcgccggc
     6721 aatcgaagca aatgtcaatg cgatcgcagc tggacatagc gcaccattta tcgtgaacaa
     6781 acctttcttc gacagctttg tc
//
LOCUS       DAPBBG010000092         6494 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPBBG010000092 DAPBBG010000000
VERSION     DAPBBG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.096"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(20..712)
                     /locus_tag="RD477_002637"
     CDS             complement(20..712)
                     /locus_tag="RD477_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7634943.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRSGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            707..973
                     /locus_tag="RD477_002638"
     CDS             707..973
                     /locus_tag="RD477_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7634944.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1149..1718)
                     /locus_tag="RD477_002639"
     CDS             complement(1149..1718)
                     /locus_tag="RD477_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7634945.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1792..2115)
                     /locus_tag="RD477_002640"
     CDS             complement(1792..2115)
                     /locus_tag="RD477_002640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634946.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2256..3080)
                     /locus_tag="RD477_002641"
     CDS             complement(2256..3080)
                     /locus_tag="RD477_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634947.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFLHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKYFTKNGFVT"
     gene            3434..4282
                     /locus_tag="RD477_002642"
     CDS             3434..4282
                     /locus_tag="RD477_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDT7634948.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4296..4619
                     /locus_tag="RD477_002643"
     CDS             4296..4619
                     /locus_tag="RD477_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634949.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4616..5367
                     /locus_tag="RD477_002644"
                     /pseudo
     CDS             4616..5367
                     /locus_tag="RD477_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            5357..5989
                     /locus_tag="RD477_002645"
                     /pseudo
     CDS             5357..5989
                     /locus_tag="RD477_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6118..>6494)
                     /locus_tag="RD477_002646"
     CDS             complement(6118..>6494)
                     /locus_tag="RD477_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7634950.1"
                     /translation="RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCP
                     FDNAVAESTYKSLKVEFVYQYTFETLQQLDSELFDYVNWWNHLRLHGTLGYETPVGYR
                     NQRLAQRILDNELGCANASEAV"
BASE COUNT         1853 a         1283 c         1177 g         2181 t
ORIGIN      
        1 aaagaggggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc ttgtcatgaa
       61 aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc atcatgacgt
      121 atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca acatttccta
      181 aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt actctagcta
      241 gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa ttagcaatgt
      301 gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca atgtgggcgt
      361 aatttgttcc atctgtcagc gtagaatttg cagcacctga tcgagttgga tcaattactg
      421 gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa tcataggcta
      481 gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta tgatcgcccc
      541 aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa ggagtgtcta
      601 atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg tatactttat
      661 agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt
      721 tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa ggaaaacacc
      781 aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt tagaagatat
      841 caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc atatttctcc
      901 cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc aaataaaaaa
      961 gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact aattttattt
     1021 ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa
     1081 ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca
     1141 tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc catcaattga
     1201 gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg cggttttctt
     1261 cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc cacgactccg
     1321 ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc tagatttttt
     1381 gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag ctcttccatc
     1441 ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt tatttctttt
     1501 ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg caagagcgtt
     1561 tcgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa tatatatacg
     1621 ccagttcccg cgtctcgcgc ttgagcaaat acatccatcg gcgatcgttt cacttctgtg
     1681 atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc tttactaatc
     1741 gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt
     1801 tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg aaggatggta
     1861 cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg tgatcactag
     1921 atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc gttttcgatg
     1981 tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt catagcgaaa
     2041 aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt cctggagtgt
     2101 tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt ggatcgctag
     2161 ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt cctgataata
     2221 acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagcc attcttggta
     2281 aagtactttg tcagctttga tttgctttcg ctctcaaatt cgctattcag ccaaaacagc
     2341 gaatcaagct tttcagtcac aatcgtcaat ttccgatagt cttgtgtctc ttctatccgt
     2401 ttccaccgct tttcagtaat ctggttacag ctcaagcgca aaattgctag ataatttttt
     2461 tcttttattg gcaaaatctg ctcctctcta aatagcaatt caggaaacaa ttcaaagaat
     2521 agtgttcgac tgtctcctaa aatctcgatt tctgccggat cctttctttg cttcaataat
     2581 tggatgaaca tctcagaaaa ggactgctct ccatcatctg caaaaaaatg cagaaaatct
     2641 ttcaaaaagc aaaacagcag atatcttact tttctttctg aaccaaagat ctgtgctttt
     2701 ttataagaaa aagaaaggtg gtactcgcca aaccactttc tcaatcgctc ttgcgtagaa
     2761 cgatcgtact tctgctcgta aggttcattc aatagcagat ttttcagtaa acaaaacggg
     2821 ggtgattgga tcagaaaaac aaacaagaga taaggttcgc tatatccttg atcataaact
     2881 aactgaacgg ttttcttatg aagaatcagc tgaatgggac agtcgctttc cgccaaacgt
     2941 ttcagatgcc gatccactgt tttaacatcg attttcagtt cttcagctaa atcagctttt
     3001 cgccagatca aatctttcat tagattgatt agtgaaaata aattctgtgc attttttgat
     3061 aatagttttt gccatctcat cgatcatttc tcacctgatt tctaaacata ttgtttgatc
     3121 aaaatgggtt cactcctaaa aagataaaaa actccctttc tatttattta aatacgcaaa
     3181 tcttcctcat tattttttat tttactcaat ttaatcgtat tctcatttca aaacgaatgt
     3241 actttaaaaa aacataataa gatcactttg cttgtaggat gtattccctt ccattacaat
     3301 gaaataaata gcgattttat gagaaggtga tgtgaaaatg gaagaaaaaa tagaagaatc
     3361 caaacaaaaa atcgatcagt tgatataaga attagaaaaa gcggtcatcg gcaaacgttc
     3421 aactatccaa ttgatgatcg tggccctatt agcaggtggt catgtcttat tcgaagatat
     3481 tcccggtgta ggtaaaacgt tactggtcaa agcactagca aaagctgtac agggaacctt
     3541 cagcagagtc caatgtactc ctgatctgct gcctagtgat attcttggtt tttccgtata
     3601 caattcacaa tcaaaagagt ttgaatttcg tccgggaccg atctttacca cgatcttgtt
     3661 ggcagatgag atcaatcgta cgactcctcg tactcagtca gcacttttag aagcaatggc
     3721 tgagaatcat gcaacgatcg acaacaatac ataccctcta gacaatcatt tttttgtctt
     3781 agctacgcaa aatccaatcg agtacgaagg aacatatcct ttgccggaag cacagctcga
     3841 tcgtttttta ttccgactac aaatcggtta tccttctttt gatgacgaac ttttgctact
     3901 tttggataaa ttcgaaaaag tgttggataa cttaaatgaa gtattgaaca gttatgaaat
     3961 agaagaaata aaaacaaatg tggcgaatgt ctttgtcagt cccgaagtgg catcttatgc
     4021 cttgcagtta gtgtcagcaa caagaagtca tgctgccatc cagcttggca tcagtccacg
     4081 aggaagcctt tcctttattc aggcagcaaa agcttatgct ttaatccatg ggagaaatta
     4141 cgtcacgccc aaagatttgc aggatttagt tccttacgtg ttttcccatc gcctgatttt
     4201 ttacgatcgt tcattgaagg aagacgaaaa gaaacaaatc attcatactc ttgttgcaca
     4261 aattcctatt cctgtgaggt agattgaggt agacaatgga aaaaatcaaa gattatttca
     4321 ttaattttgt gttatttctg gtatatttca tcattgtgct ttttgctctt gtattcaaca
     4381 attctttagg ctggttcttg ttggcctttt ttactcttta tgtatcatgg agcttgattt
     4441 cattaagcag ctttctaaag aaactttcgg ttacttgtac attgcagcca ctcgtttatc
     4501 gcaaaaaaag tatgcaggtt attttttcca tagcaaaaaa gagaactttt tctttacctc
     4561 tccctcgttt acaaatcggc ttcccagcaa tctttgcaga aaaaaacaag agattttgat
     4621 tcttacaaat aaaccgcaag aagtccaatt gctttggcag ccaaaagaaa gaggatgcca
     4681 tgaaacatta actgtcattt ttacagccta tgatagtcta ggacttttca gaaaaagtgc
     4741 aaaaagagag atctcttttc ctgtgactgt tctgcctgct ttttgtaaga ttcaagcaga
     4801 aaaattacag agagttctgg aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgctcagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggtttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagtcacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacact ggaatggcaa
     6001 cagttttttt gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt
     6061 ccttgacaat gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta
     6121 gactgcctcg ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaatct
     6181 ctggttacgg taaccaaccg gtgtctcgta gccaagtgta ccgtgcaacc gaaggtggtt
     6241 ccaccaattg acatagtcaa ataactccga atccaattgt tgtaaggttt caaatgtgta
     6301 ttgatagaca aattctactt tcaacgactt ataagttgat tcagctacgg cattatcaaa
     6361 aggacagcct ttatgactca atgatcgatt gatgtcaaaa gttgttaata attcatcaat
     6421 agcttggtta tcaaactctt ttccacgatc agtatgaaaa atctcaacct ctgtcagagg
     6481 ttgtttgata cggc
//
LOCUS       DAPBBG010000093         6422 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPBBG010000093 DAPBBG010000000
VERSION     DAPBBG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.097"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1030)
                     /locus_tag="RD477_002647"
     CDS             complement(<1..1030)
                     /locus_tag="RD477_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7634951.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="RD477_002648"
     CDS             complement(1112..1801)
                     /locus_tag="RD477_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7634952.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="RD477_002649"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="RD477_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDT7634953.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="RD477_002650"
     CDS             complement(2328..2831)
                     /locus_tag="RD477_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDT7634954.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="RD477_002651"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="RD477_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDT7634955.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="RD477_002652"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="RD477_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDT7634956.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="RD477_002653"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="RD477_002653"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDT7634957.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="RD477_002654"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="RD477_002654"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDT7634958.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
BASE COUNT         1893 a         1322 c         1219 g         1988 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 tt
//
LOCUS       DAPBBG010000094         6319 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPBBG010000094 DAPBBG010000000
VERSION     DAPBBG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.098"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            58..477
                     /locus_tag="RD477_002655"
     CDS             58..477
                     /locus_tag="RD477_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDT7634959.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            489..959
                     /locus_tag="RD477_002656"
     CDS             489..959
                     /locus_tag="RD477_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7634960.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            976..1755
                     /locus_tag="RD477_002657"
     CDS             976..1755
                     /locus_tag="RD477_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7634961.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            1742..2563
                     /locus_tag="RD477_002658"
     CDS             1742..2563
                     /locus_tag="RD477_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7634962.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            2580..3629
                     /locus_tag="RD477_002659"
     CDS             2580..3629
                     /locus_tag="RD477_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7634963.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            3642..4646
                     /locus_tag="RD477_002660"
     CDS             3642..4646
                     /locus_tag="RD477_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7634964.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            5279..6298
                     /locus_tag="RD477_002661"
     CDS             5279..6298
                     /locus_tag="RD477_002661"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDT7634965.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         2080 a         1024 c         1382 g         1833 t
ORIGIN      
        1 attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg
       61 gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta
      121 gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat
      181 tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt
      241 gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg
      301 gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg
      361 ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa
      421 atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag
      481 gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt
      541 tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta
      601 cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga
      661 tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt
      721 tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa
      781 tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag
      841 cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag
      901 aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag
      961 aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca
     1021 caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt
     1081 acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta
     1141 gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg
     1201 attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta
     1261 ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt
     1321 ccaattatga accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag
     1381 catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt
     1441 tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt
     1501 caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct
     1561 cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct
     1621 tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta
     1681 gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa
     1741 gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt
     1801 ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta
     1861 tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc
     1921 agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt
     1981 aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc
     2041 gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt
     2101 ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa
     2161 tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta
     2221 tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa
     2281 cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc
     2341 aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc
     2401 tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt
     2461 tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact
     2521 agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa
     2581 tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa
     2641 agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa
     2701 cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc
     2761 ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa
     2821 tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa
     2881 aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac
     2941 ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg
     3001 ttacaaaagg ctattctgcg actgttttac agttactact cttagggata ggaattggaa
     3061 taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa
     3121 taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa
     3181 gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag
     3241 ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt
     3301 tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta
     3361 ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt
     3421 acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat
     3481 tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat
     3541 tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact
     3601 ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag
     3661 aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca
     3721 tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt
     3781 atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg
     3841 tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg
     3901 ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca
     3961 agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc
     4021 tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg
     4081 cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac
     4141 agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg
     4201 gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga
     4261 atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc
     4321 catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa
     4381 caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg
     4441 ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag
     4501 tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag
     4561 tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc
     4621 gctactatcg tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag
     4681 agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa
     4741 acacattttt tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg
     4801 ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag
     4861 tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa
     4921 acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga
     4981 tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt
     5041 cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac
     5101 aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat
     5161 ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca
     5221 actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat
     5281 gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt
     5341 ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat
     5401 caataagtat catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat
     5461 cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc
     5521 aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat
     5581 cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt
     5641 ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca
     5701 tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa
     5761 agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa
     5821 tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg
     5881 cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt
     5941 ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt
     6001 gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc
     6061 cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt
     6121 cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga
     6181 gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc
     6241 agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat
     6301 agatgatcaa tagttaatc
//
LOCUS       DAPBBG010000095         6225 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPBBG010000095 DAPBBG010000000
VERSION     DAPBBG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.099"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            56..508
                     /locus_tag="RD477_002662"
     CDS             56..508
                     /locus_tag="RD477_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634966.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            522..1211
                     /locus_tag="RD477_002663"
     CDS             522..1211
                     /locus_tag="RD477_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634967.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1336..3687
                     /locus_tag="RD477_002664"
     CDS             1336..3687
                     /locus_tag="RD477_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDT7634968.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3767..4162
                     /locus_tag="RD477_002665"
     CDS             3767..4162
                     /locus_tag="RD477_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634969.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4487..5104
                     /locus_tag="RD477_002666"
     CDS             4487..5104
                     /locus_tag="RD477_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7634970.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5145..5444
                     /locus_tag="RD477_002667"
     CDS             5145..5444
                     /locus_tag="RD477_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDT7634971.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5478..5645
                     /locus_tag="RD477_002668"
     CDS             5478..5645
                     /locus_tag="RD477_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7634972.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5690..6214)
                     /locus_tag="RD477_002669"
     CDS             complement(5690..6214)
                     /locus_tag="RD477_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7634973.1"
                     /translation="MDKLFQEKASGKDTERPEFQKLLNYIREGDCVIVTSLDRLGRDY
                     EDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIR
                     ERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGI
                     SRPTIYKIKKANDL"
BASE COUNT         2142 a          858 c         1244 g         1981 t
ORIGIN      
        1 attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga gaatcgtgaa
       61 aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct attttctttt
      121 gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag ctgaacataa
      181 taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag aattgtataa
      241 agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa ttagtgactc
      301 agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca aatccgaatt
      361 agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt ccaacgctat
      421 ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa agcagataaa
      481 agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag atttttggtg
      541 tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct gctgagaaag
      601 aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt gaagaagaag
      661 ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa attgtatcta
      721 tggataatcc agatataatt caaggttcat ataattcaga aactgatcct gcttcagttt
      781 cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt gaaactactc
      841 caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac cttcttgtac
      901 tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg cgttttggag
      961 gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa tcctacattg
     1021 acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt tctattcaag
     1081 gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg cctggttgga
     1141 caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat tatgttgttc
     1201 aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta tttttagttg
     1261 ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg ttcggcaaga
     1321 aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct aaacaagaaa
     1381 tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg gtggcctaca
     1441 atctatggac ttattgaagg actacaagta gaacttggta ttactaatct agtagctaat
     1501 tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg gggcaaaaat
     1561 ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg gattaatcca
     1621 ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga gttgcagact
     1681 gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg ggccaaagcg
     1741 ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat tagaaaaatg
     1801 cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg tgatggtatt
     1861 tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact aggatattct
     1921 cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta tcctgtgtct
     1981 gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt taatggttat
     2041 tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga agtactggca
     2101 ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt tgtaataaaa
     2161 ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga tacttctacg
     2221 caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat agttggtaga
     2281 tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct aactacagag
     2341 gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca agatggaggt
     2401 gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa ggctatagat
     2461 gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt tgatctagac
     2521 atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt atctgctgta
     2581 atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg ttctcaaata
     2641 atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg ttatagtggg
     2701 aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga atttaccata
     2761 ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag agatagcgga
     2821 tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata caatcagaag
     2881 gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct tgataatcct
     2941 tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc atgggacatc
     3001 atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag gaataaatta
     3061 actaatagcg agggcctgta taattatttt atagatccta atactggttc aataataggt
     3121 ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac aatcaatgat
     3181 agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg tgaagttaag
     3241 aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc tgcaggtgga
     3301 acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta taccttagca
     3361 aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg tgataagggt
     3421 gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga taaaatcgct
     3481 tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg tgtagggata
     3541 atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt tacttatctt
     3601 gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat tattcaattt
     3661 caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt gtccttttcc
     3721 tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga tgattatgga
     3781 agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat gtggaattat
     3841 ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca agatagtaga
     3901 ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt ttgaaagagt
     3961 accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag agtctccaaa
     4021 tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa agaaagaata
     4081 caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta aattagaaat
     4141 atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa gaagtcaaat
     4201 tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg ataactagtt
     4261 tatttaatgg cagaccgtta aggcctgcca tttttatgta ttttagcgga ataaatataa
     4321 tcatgcggga gtgtaaactt actgtttcaa aaagtatact ttttgacatg ccttgatttt
     4381 ctttgtttat gtatggcata ataaccacat aatacaattt gaaacgtttc tgatttaatc
     4441 tgattgtttt gaaacaattt tagtgaaaaa agggtagata aaaacgatga gaaaattttt
     4501 ttatatccgg gtcagtagtt atgatcaatc gatcgaacga caactgattt ccgcgaagga
     4561 attaggaatc cctgaagaat atatatttgt agaaaaagcc agcggcaaag attttaaacg
     4621 ccctgaatat caattaatga aacggatgtt tcgaaaagga gatttactct atctacaatc
     4681 attagataga ctaggcagaa ataaacaaat gattttagaa gaatggcgag aattaactaa
     4741 ggaaaagcag attgatattt ctgtgttaga catgcctctg ttagatactg ctagttacaa
     4801 gcaactagat gggttagaga cgcttatctc tgacttaatt ctgcaattac tcagctatat
     4861 ggccgaagac gagcggaaac gaattaatag ccggcaaaga gaaggcgtag aagctgcgaa
     4921 acaaaagggt gtaaaattcg gcagaaagaa aattgcttta cctcctgaat ttcctgcgat
     4981 ctattctgac tggaaagcag gaaagataac tgcagtctat gctatggatc aattgggcat
     5041 gaaacgaaat accttttatc gacgggtgaa agaatatgaa cataccttgc accacttttc
     5101 ttgatagata taacagctat tagacgggaa gggagagttt gaaaatggac ttaacgaatc
     5161 aaagggtcgt ttttactgga acgctgcagc aaatgacacg aacccaagcc atcggtttgg
     5221 ttcactcttt aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat ttcctggttt
     5281 gtggtcagta ttctaagtcg ttgtttgatg attctctcta tacaaaaaag gaacaaaccg
     5341 cgcgggactt gattgcgtta ggtcatgaga ttaccatttt atcagaagtc gaattttatg
     5401 cgttaattag tcaacagttg aaagaatggc aacggtattt ataatcgtta gtaaaattta
     5461 caaggaggaa aatttttatg aagcgaaaat cttatgatcg ttggggcatt gaaataacta
     5521 gttttaatcg aaatattgct tttcacgtgg agtgtgactg tggggaatta gcaaaaagtg
     5581 tttatcaaaa aaattacttt tactgtccta aatgccatcg ccgatacggg gtacatggaa
     5641 catagaaacg aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc tacaaatcat
     5701 tcgctttctt tattttataa atagttggtc gagagatccc gttttctttt gcaatctctg
     5761 caactggaat gccttgcttt aacatgtcca ccacggtttt aaaaactaag cgtttttgcg
     5821 gatctgttga gttaatagaa tactcgattg gtcgtccctt atatcggccg gctttttgag
     5881 ccaataatac tccttgccgc tgacgttcac gaattttctc acgctcgttt tgtgcaatat
     5941 aacttagcag tgataaaaac atatcgaaca tagcggtatc caggagttca tttccagtat
     6001 taaaatctaa aaactgtgca tctaatattt ttaacgctac ttttttctgt ttcataaaag
     6061 caactgtatt ttttatatct tcataatctc ttcctaaacg atctagtgat gtaacgatga
     6121 cgcaatcacc ttcacggata taatttagta atttttgaaa ctcaggacgc tcagtatctt
     6181 taccactggc tttttcttgg aataatttat caatgttaag tcctt
//
LOCUS       DAPBBG010000096         6170 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPBBG010000096 DAPBBG010000000
VERSION     DAPBBG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.100"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..567)
                     /locus_tag="RD477_002670"
     CDS             complement(<1..567)
                     /locus_tag="RD477_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634974.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVT"
     gene            complement(812..1726)
                     /locus_tag="RD477_002671"
     CDS             complement(812..1726)
                     /locus_tag="RD477_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDT7634975.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(1708..2088)
                     /locus_tag="RD477_002672"
     CDS             complement(1708..2088)
                     /locus_tag="RD477_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDT7634976.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(2538..2825)
                     /locus_tag="RD477_002673"
     CDS             complement(2538..2825)
                     /locus_tag="RD477_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7634977.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(2845..3069)
                     /locus_tag="RD477_002674"
     CDS             complement(2845..3069)
                     /locus_tag="RD477_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDT7634978.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(3524..3694)
                     /locus_tag="RD477_002675"
     CDS             complement(3524..3694)
                     /locus_tag="RD477_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634979.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(3709..4242)
                     /locus_tag="RD477_002676"
     CDS             complement(3709..4242)
                     /locus_tag="RD477_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDT7634980.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(4263..5207)
                     /locus_tag="RD477_002677"
     CDS             complement(4263..5207)
                     /locus_tag="RD477_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDT7634981.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            5844..5930
                     /locus_tag="RD477_002678"
     CDS             5844..5930
                     /locus_tag="RD477_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDT7634982.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
BASE COUNT         1633 a         1153 c         1040 g         2344 t
ORIGIN      
        1 ggtaactgat tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc
       61 actgaataaa ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt
      121 tttggttcgc tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata
      181 aagacgagtg agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat
      241 atcgttcatt tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc
      301 ctttttcttt ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact
      361 tcttgaaata cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa
      421 tgtaaatata acccctggca acaaaaacct cttaaatcga attttagagc cttttaatga
      481 atatcttgtt tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat
      541 gagtagatag ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc
      601 taacagtgtc atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat
      661 tacttttttc tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg
      721 tgaatcaatt gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt
      781 ctttatttca ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg
      841 ctcctctttc aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag
      901 tcgtaagagt tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag
      961 ctcttcgtag ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat
     1021 gtaaaatcgt ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt
     1081 tgtggatcaa ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt
     1141 taagagaatc acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa
     1201 tcgcaaaccg aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag
     1261 acatggttcg tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac
     1321 aaatctcgtc atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt
     1381 tgttcccagt ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt
     1441 ctgtatgtgt gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc
     1501 ctagttgatt ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt
     1561 gtgcttgtac acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg
     1621 cataatcaat gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga
     1681 tcgctcggct acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc
     1741 cttttgcatg atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg
     1801 ttggtgtgtg ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc
     1861 gcgcgcgatt gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg
     1921 tgccttggct ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag
     1981 ctttgcaaat tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt
     2041 atatttttta gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct
     2101 ttcagtacgt cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa
     2161 gtaaagctca ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac
     2221 ctacgtattt ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc
     2281 ctccccccca ctctccacaa tacaaaaaag actagcaaat gctagccact ttttgtaaag
     2341 tttcgcttgt ttcaccttcg gtgaaattgt tgcaaatcaa taattaggta gagctacatt
     2401 tttttcgttg ttcaaagatt cttatgccat ttactattaa taaatctata aatatgataa
     2461 tttttcggct ctataattta tgggactgat gaatcagaat tgattcttca gtcccatttt
     2521 cattttattc caaagaatta actccaaata ccaatagaag cccatcctct atatacttga
     2581 gattccttac taacaatttc ttgtaattct attaaagatt taggtaatcc aaatttatgt
     2641 tttaaaatat atcttccaag aaaactactt aatttcatac taacttcacc aacacttttt
     2701 ccctcactga tttctttaga ataaagaaag agcttttctt ttaagtcagg atagtcatct
     2761 atagttgctt cattgtatac tttactaatt gcatttaaaa gtttttcttc tttagtaaaa
     2821 tccattataa taacctccct accactacct tctaggtgcc catggaccat gattaaccca
     2881 accattaaca ataatttttc caatttctcc tttagcttga ttccaatcta cccaacattt
     2941 ttctttgtta caataaagac catttccata gtaagtagct gcatcaactt ttattcctgt
     3001 accgatgcca gctagacaag ttcctaatat tcctagaata acacaatgtt ttaatacttt
     3061 ctttttcatg attgtttttc cttttcgttt ttttaaaatt gcttgttata ttaaataatc
     3121 ttagtaattt tattatataa cattttatta tataacaatg attaacgtca taaaaagtag
     3181 ttttgaattc ataatcgttt aaataaaatt ataagatcag ttatttaaac agctagtaga
     3241 cataatgtac atactaccaa gtaacgcctg taccaaggga tccaggcgct gattttgtga
     3301 atttttattg gtctaggtta aaagctcttt ttaaggcttg gttttctgcg gttgtttctt
     3361 tctaattctt tgtgaatatt gatcggtcat tttttaaata cttgttattt ttgtttgtgt
     3421 ttgttatgct ttttacatat ctcatattca tttattattc ttttctaagg ttaataatag
     3481 gataatctct ttcagaaaga ctgctcgtac gagcagtctt ttcttattta tcaagttttt
     3541 ctacaaatac ttcctcattt tgactgttgt agtaattagg attgagtttt agttcccctt
     3601 ttttatttcc tttgttggcc actgataata acattgggct aaagcttaat attcctagta
     3661 ggatagaacc gaatagcaag tgtttttttg tcataagtat ttccttcttc atttccatag
     3721 ctgccaccat tttttccaat tctttgtttg ttcgtccatt gtttctgttg cttcagaaag
     3781 agtcgttgaa ctattctctg aagactcttc cgtataagtc aactgcagtt gctcctgaag
     3841 tttttcgatt tgttggttgg cttgcagggt gagttgttgt tgctggtcga gtagtttttg
     3901 tatatgagaa ttattttgct ctttgagggc aagttgttct cgtaaaaatt cattttcttc
     3961 acgcaactga tctagcaact tgtttgcaac cacgtttttg tttggtatat aagcattttt
     4021 atcgttgttt tgttcatgtt tatgcaactt atctagcaac ctttgattgt ctaagttgcg
     4081 agcaagttgc aatactccag aacgcaatac aaccttataa ccacgctcat ttttagctat
     4141 catggaagaa tctaattttt ggatatgttt cgatattgct tgcctactca tcttaaaaag
     4201 atctgacaat tcctttattg taaaggtttc ttgtggttgc atactggttg cctcctaggt
     4261 tgctactgga ataaattatc taaatcagat aataaacttt ttttctcttt ttccgaaagt
     4321 gcggactctg tttgtttttc aagagctttt ttctcttttt ctagttgttt gatttgttgg
     4381 aggagttcaa gttctcgttt cttctgtttg attttttcgt taattgttgt agcgggttct
     4441 tcgtattctt tttgtttatc agctaaagcc aatgctttat tttggtcttc tttggttggt
     4501 gcatgtttgt taaagtcgtc tgcttttttg atctcaggtt tgaacgtaaa ttgatagcca
     4561 ataacaggtt ttccccgtcc ttttccgtat ttctttttga ttgttagtcc tttgaagatt
     4621 gcagttagtt cttgacgtat aggttttata actcgtttat ctatatcagg ttgcttataa
     4681 cttttaggaa tatctaataa ttcaagaaag tcttctttgg agaaatatgc gaaacctttg
     4741 gttctgtatt gctttaacag acgaaacata gtctttgcat agccactttg tagctcgttg
     4801 aattgttgta aagaatagcg tacccactcg tctaaattat ttaacaaagg cagtgctttt
     4861 tcgtaaattc gtatatctac gtaaggattt tctacgtccc cgttgatttt aaattctgta
     4921 aacattacaa acatatcgta agaaagccca gactcacttc tacgaccaaa gcgtaaagat
     4981 aagatttttt cataagttgc ttttaaatct tctacaaatc gtacatttgc agttgctttg
     5041 tagttactta aatcttttag ttggtcaaac gtaaaacgaa ctgtcttatc ccctttttca
     5101 cgcattctag acacgatgga aaaaaacaaa ttcatttcaa ccggtgtaaa ttttcttaaa
     5161 ggaattgtat ttaattcatg gtggtatttt acaatctcat tagccatata atcacctcaa
     5221 tccaattatg aatgacattt tttaaatgtc aagacattca tatttgacgt tcaaacagac
     5281 aaaatgtcct tcataaacag acaaaatgtc cttcataaac agacaaaatg tccttcataa
     5341 acagacaaaa tgtccttcat aacattctgt atccattggt agagtaagga cacagcttac
     5401 ggtaaagagg tttaaaaaga ggtttaaaaa gagatataaa aagagggggg ctaattttcc
     5461 cgcctaaaaa ccacttcttt tttctttaaa caggttcgcg catttttcaa aactgccaat
     5521 ccaaaacctt gcttcctacg gtcgccaaaa aggtgtgatc gctacgctct cacattgtac
     5581 tgcgcagtcg cctaaaggct cctttgctaa aaacatgttg gtaaagaggc ctttgagcgg
     5641 ttctgaatgc ttatctaagc cgttttaaga cagtcttgga taaaaatcct tatctagctt
     5701 taaagcgcct tacaggcggt cagggcaagc cctaacaacc ctaaaataag cgatacttaa
     5761 aaacaccaca caagtttaat tttttaagat ataataactt gtcgcctatc cgacagtctt
     5821 gcgactggaa ggaggtgtat ctaatgtttg aattgttcat ttgccccctt cttatagaag
     5881 tttgtgtctc tttattcagt tattggttga ataaccgaaa caagaagtaa aatcaataaa
     5941 ggcgacatca acctgaaaaa aagccccaga gaatccgacc tctctggggc tttcgtgtat
     6001 ctaatgtttg atcatttgca tgtttagttt aacatgattg ccgatcaaat tcaattttgc
     6061 tgttgctgat ctaaccattc cttgacactt tttagataat ttaatgccgt ttcttttttc
     6121 gttggatttt gtatgttcgt tgcttgctga agcagtgctt tataacgttc
//
LOCUS       DAPBBG010000097         6130 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPBBG010000097 DAPBBG010000000
VERSION     DAPBBG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.101"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            10..324
                     /locus_tag="RD477_002679"
     CDS             10..324
                     /locus_tag="RD477_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634983.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(758..1124)
                     /gene="ssrA"
                     /locus_tag="RD477_002680"
     tmRNA           complement(758..1124)
                     /gene="ssrA"
                     /locus_tag="RD477_002680"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1295..2068)
                     /locus_tag="RD477_002681"
     CDS             complement(1295..2068)
                     /locus_tag="RD477_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7634984.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2212..2562
                     /locus_tag="RD477_002682"
     CDS             2212..2562
                     /locus_tag="RD477_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDT7634985.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2543..3259
                     /locus_tag="RD477_002683"
     CDS             2543..3259
                     /locus_tag="RD477_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7634986.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3259..4707
                     /locus_tag="RD477_002684"
     CDS             3259..4707
                     /locus_tag="RD477_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7634987.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4777..5286)
                     /locus_tag="RD477_002685"
     CDS             complement(4777..5286)
                     /locus_tag="RD477_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634988.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5276..6001)
                     /locus_tag="RD477_002686"
     CDS             complement(5276..6001)
                     /locus_tag="RD477_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634989.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2075 a         1040 c         1185 g         1830 t
ORIGIN      
        1 ggatagcttt tggattttta cagtaatttt atcttaataa tagcaatatt attacttcta
       61 aatatttggt tttttgataa gagtagaaat gcagggatag gttttagaac caaacgaagt
      121 acgtcttctg aaaaaaagtg ggtatattca cagactattt tttacggagg ggttatttca
      181 atcagtttac tttcttcaac actttattct tttaatgtta tagacgtttc catgtcaaat
      241 tttatatcta ttattggaat ccttatttct gcaattatta cacaactact tcttgtgttt
      301 gaagagaagt caaaaaataa ttaaagcaat tatgcctttt agtacaggaa ttattacttc
      361 ttacaaatgt atagcgaatg tgaagcctgc tgttttaatt gttctagaaa aacttttgag
      421 tgaacaatta ttttatgtta ggatgaatta atttctctag agtttataat taaaaaactt
      481 atttgaagga acatgcttaa accataattg tgtttttcct tccatcagca taaaagttca
      541 cctcaaaaag agtgtacgaa caaaagttct ttttgtaaac cttaaaatag attttcctga
      601 atacgtattg gggatttcta aatgtttgaa tgcttttttg aatgcaaaat caatgcaaaa
      661 aattttgcat ttctctactt ttctctcctt gaccaaaata aaaaaacgtt gatttaacaa
      721 cgtttttcat acatgtttat ctgtctcaac catacatata tggagacggc gggagtcgaa
      781 cccgcgtcca aacacatcgc cacttaaaaa tctacgttca tagtcaactc atttaaggat
      841 tcgctttaca gcttgccgag tgacaggcat tctgtatcgc tagtctgatg ttctcttttt
      901 caacttacag acggaaagtt gaaacgtatc ccactaaagt taggaccctt actcgagcac
      961 atgggcgatg ccgagaggat ctacgctaac tgtttttagg cagctaaagc gtaagagttg
     1021 ttttcgtttt tagcagttat atttaactgt aacgttttta cgtagacgta acctacgaaa
     1081 cgcaattcaa gctcgactgt gcctgtcgaa tccgtaacgt ccccgttagc taacaagggt
     1141 tacgtgtatt tttcatacaa aattagtata gcatatattg ggataaattc aataaaaaag
     1201 cgaatcatat cccattattt atataataac tgcccctttc ttaagaaaaa ataagcgatt
     1261 ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat cagatcaaaa tacttcaatg
     1321 cgtggacaat tccgccttct gtatttttct ttgtgataaa ggtagaaagt tcttttaatc
     1381 cgtcataggc gttgcccata gcgattttgt tgtcgcaagc ttccaacaag gcaaaatcat
     1441 tcggaccatc gccaaatcca aaagtaggaa ctttttccag ctgaagttct ttttgcagac
     1501 gtttgacacc tgcccctttt gagattcctt tcttcactgt atcgatcgag taaggaccat
     1561 tccgataaaa agttagctca ggaaaatgtt cgtgatagta ttcgtcacct gattgagata
     1621 agatcagcaa catatttact gtgttttctt taaagcctaa aggatcaatt tccggaacat
     1681 ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct atgaccggta caccagattt
     1741 tctgatcgtt atagtaagaa agttcatgac cgttcaactg tacatgatca tacatttttt
     1801 gacattcttc tgaagaaaat gtttgagaat agatttcttc gccgtctact cgaatgaaag
     1861 aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc catgatagag tggatttctg
     1921 cttctgtacg tcctgtagcg atgacgggga gaacattgtt ttcttttaat gcgttcatag
     1981 cttttgccac ttcgggtgtg atttttgatt tcgcatctag taatgttcca tcaagatcaa
     2041 agaaagtgat tgcacgataa tttgacacag ataaaactcc ttttttattg gaataagttt
     2101 atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa gaattcctga acatattggt
     2161 tcagtcaaca atttagagta tactataaga agagaattta ggagggaaag aatgattttt
     2221 ttcagtattg gtatcttatt cttaataatt ggcagtcttt ttcggattct gccctcaaaa
     2281 ggaaatctgc ctttttatgg gtaccattcg cctcttgccg caaaaacaga cgctcattgg
     2341 cggctggctc aaaagacaag cggaaactgg ttttttctta tgggcttact gatggcattg
     2401 atcggctatt atttaaagac atctgggcat acgaattatt ttctgattga aatgcttttg
     2461 ctagtatttc caattatgcc tatttttatt atgacagaaa agaaattaca gaaatatgac
     2521 ctggaaactg gagggaacga taatgaatat tttaatgatt gaagataatc aatctgtttc
     2581 tgaaatgatg cagatgtttt tcttaaatga aggttgggaa gcaactttca agtatgatgg
     2641 aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat tgggatatga ttacactaga
     2701 cctgaatttg cccacactgg acggtatgct agtaggaaga gagatccgca aagtttcaag
     2761 tactgtgccg attatcatgc tgacagctag agattctgaa agtgatcagg tgatcggctt
     2821 agagatgggg gcagatgatt atgtcacaaa acctttcagt cctctgacgc ttattgctcg
     2881 aatgaaagct ttgcatcgtc gtagtgagtt ggcagaagca agagaagaag cagcagtttc
     2941 agatgatcat tttgatatcg agactagtca tttcaagatg aatacgaaaa cacgagaagc
     3001 gtatctggac aaccggccaa ttgaaggact aacgcctaaa gagttcgatc tattgtttac
     3061 tttagcaaaa aaaccacgtc aagtattctc tcgggagcag ctgcttgaat tagtctggga
     3121 ttatcagtac tttggtgatg aacggacagt ggacgctcat atcaagaaat tacgacaaaa
     3181 aatcgaaaag gtcggaccac aagtcatcca aactgtttgg ggagtcggct acaaattcga
     3241 tgattctggt gttgcctaat gcgttatttg tatcagcaac tcttagcttt ttgcggaatg
     3301 gtcgcgttga tcttattgat cgttggagtt tcttttacac aactgacaaa acaaacgatt
     3361 gaagaaaaca attaccagca actgtttgga tatgctgaat cagttgaaaa gacaacgcaa
     3421 acctatgccg atacgctccc gcagtttagt caagatcagg cgtttcaaaa tgcattgctg
     3481 ctgacagagc aagtactgac agaacaaaat gtgaacttta tatttatcga taaaaacgaa
     3541 cgtgtcattt atccgactaa tacagcttta ctgaatttta ccttaacagc tgatcaatgg
     3601 aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga ataaaaatat tttaggacaa
     3661 gcacaggcga cctcttacgc acttgtgcca tttaatttga atcatgaatt ctatggagca
     3721 cttgtagtta gtcaacctgc gcgaaatatc gataacagcg ttcgtccaat tacactgaac
     3781 ctgttcaaag gatttatttt ttccagtatc gttgcagtta ttgccagcta tgcttttgca
     3841 gcattccaag ttaagcgtat caatcggtta aggaatgcga cgaaagaagt gacgaatggt
     3901 aattttgatg tacagctccc cgtacatgac aaagatgaat ttgatgagtt agcagatgat
     3961 ttcaataaaa tgacgaattc attgaaagaa tctcaagcag aaattgaaga acaagaaaat
     4021 cgtcgaagac aatttatggc agatgcttct cacgagatga gaacacctct tacgacaatc
     4081 aacggacttc tggaagggtt agaatacaat gcaatccctg aaaatcagcg agaaaacgcg
     4141 attaaattga tgaaaaatga aacagagcgt ctgatccgtt tggtgaatga aaacttggat
     4201 tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta aaaaattcaa tggaacagaa
     4261 acattgaaaa atattgttgc acagttagaa gctaaagcag aagcagcagg agatacattg
     4321 acattgaaag ccgttgatga tattgatgtt tacgctgatt atgaccgatt tgtccaaatc
     4381 atggtcaata ttattcaaaa cgcgatacag tttactgaaa acggccaaat cacgattact
     4441 ttagaaaaag gctatcttga aacgatcatc acgattgaag atacgggaat tggaatgtct
     4501 gaacaacaaa tgaaaagtat ctgggatcgt tattataaag tagatccttc aagaaaaaat
     4561 acaaaatatg gtgaatcagg attaggttta ccaatcgtgc agcaacttgt acgcttgcat
     4621 aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa caaaattcgc tgttcgtttg
     4681 cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta taaaagatca tgaatcgatc
     4741 ttctataccg cttgttttta ctataaaatg attgatttag cccgcgggct ctaaacgatc
     4801 aatataagga ctttgagcaa tagtagcatc atgagtaaag agtaagatac cttgattttc
     4861 ttcatgagct aaataagaaa gcaagtggag tagctcttgg atctccaata cactaagttc
     4921 atcaaatgta tcttcaataa caatattgtt tcgcttggaa agcaaggcat gaagtaattg
     4981 cagctttact ttttcaaaag acgtcaactc agctgccgca caattaagca aggaaggaga
     5041 gatatcaagt ttttcttgat aggccaaacg atcttgtttg atttcttttt ccggtacatc
     5101 caacaggaca tggtctttaa gattcagata aggaaacatc ggccaatttt tttggatgat
     5161 cgcaaaatta tccagttttg caacaagaac ctcttttttc aagtgctcca atattttatg
     5221 ctgtttttcg agaggacaca aaataacggt caagtgttca gcatgaatca aatattcagg
     5281 tttcattacg tccaggcctc ctactttgca aatgatggat aaaaaaagtg aaagggaata
     5341 gactaagaat gagacagcat ccactgagcc caataagagt ttgggcaaat cccttaagta
     5401 tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa aaaagtctgt tgatccatcg
     5461 gaattgtaag tccgcttacc gaagtcaaga tatcacggat atctggcagt tcagtagtct
     5521 ttaagacaag aaaattgatc ctttgaagaa gcgcttcata aactgactgg aagctgacga
     5581 tccacaataa aaccaagata caacaaagaa agagaggaaa gaacagctgc cagaaaataa
     5641 aaaggaataa tttggtttta gaaagaccag tcaggcgcca attgatcaat tcctgcttgt
     5701 tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa ggcaaataga aaaaataaac
     5761 tgaatatgcc ttttatataa aatcggctag ctaattctaa agccggaaga agctctgtag
     5821 atgcagaagc gccaataaat gaaagccgat cttggacttg attggctaaa gccgattgcg
     5881 aatggtaaag agtcaggcat gctatcaata aaaaagcaga aaagcttgaa aataaacaat
     5941 agagtattgt ctgctttttg taataaatta atcgagcagt agcatagcgg attattttca
     6001 tacttttcac ctctataaaa agtatagaaa atgaatatga ataaagtatg aaattccttt
     6061 agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa agcaagaggt tgtgaagaag
     6121 ctgccctatt
//
LOCUS       DAPBBG010000098         5896 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPBBG010000098 DAPBBG010000000
VERSION     DAPBBG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.102"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(976..1149)
                     /locus_tag="RD477_002687"
     CDS             complement(976..1149)
                     /locus_tag="RD477_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634990.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1152..1550)
                     /locus_tag="RD477_002688"
     CDS             complement(1152..1550)
                     /locus_tag="RD477_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634991.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1766..2821
                     /locus_tag="RD477_002689"
     CDS             1766..2821
                     /locus_tag="RD477_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDT7634992.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2841..3194
                     /locus_tag="RD477_002690"
     CDS             2841..3194
                     /locus_tag="RD477_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634993.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3376..3672)
                     /locus_tag="RD477_002691"
     CDS             complement(3376..3672)
                     /locus_tag="RD477_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634994.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3676..4494)
                     /locus_tag="RD477_002692"
     CDS             complement(3676..4494)
                     /locus_tag="RD477_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7634995.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4720..4941
                     /locus_tag="RD477_002693"
     CDS             4720..4941
                     /locus_tag="RD477_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDT7634996.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4943..5209
                     /locus_tag="RD477_002694"
     CDS             4943..5209
                     /locus_tag="RD477_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7634997.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5322..5861
                     /locus_tag="RD477_002695"
     CDS             5322..5861
                     /locus_tag="RD477_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7634998.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT         2064 a          982 c          874 g         1976 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc atttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaatcattt attatgctct tttataagga tattaattga
      481 tctaatttga aaaaaatcaa aatttaaatg ttttaatatt acaaataaaa tcatgttatt
      541 gtaaaatggt ataaaaaagc taaatattct atttttgtta ttttattagc aggaaaattt
      601 aaatagtttt acaagaatcc gtagaaatat attggttttg ccttcagatt aatactggat
      661 catattaatc tgaatataat atatgaacta ataaatagct taacctcgct attttgtttt
      721 ttctgatcac tatctataca gcattagtaa atagaatttt aaaaataaaa aaattaagac
      781 aaaaaagtat caacataaac atatacatat acacgtttat gttgattcac ttaatttgtc
      841 ctttaaaaag cacttatttt aatataatgt tattttataa tcttattaca agctgattgt
      901 taggcgttgt cctttcatta catttattaa cggttatttt attgtttaat ttattaatta
      961 tttttgatct aaaacttagc ttaacaaata aataattaac caaccgccag ataaaaatgc
     1021 gatcattgct aatacataag caaagataaa aatatgttcg atcagcttaa accctgatgc
     1081 gattaagcag accaagtaaa atagaaattc tcttacagat aatggtaatt ttctaactag
     1141 catactcata attaacaccc caaagtaata ttgtattttg aacattttac atccaataca
     1201 gcatcaatca accattgcca atcaaaaata caaggcttca ttgaagtatg tttttcagat
     1261 gaaaagatat aatataattt cttcggaata atattttcag ttgttgggat caagctaaat
     1321 ctaataccgt ctaactcata gctaccagct ttctctccat taaaaatttc cttagcactt
     1381 tcttctaaag aatcaaaata tttttcgaca gcaatctttg aagcttttac tattgaagca
     1441 tttccctttt cgataaaatc tgcttttgtt ttaccgacaa taagtaattc ttgatcagta
     1501 aaatcttcta aaataatggt agtgtaattc ttatttcttc tcaatcgcat caggatcact
     1561 ccttaatata atttatgttt ttataatatc ataataagat aaatttgcaa gcatttattt
     1621 acaccttttt atttacactt tattataatt aattagactt ttatgattgc atttttttct
     1681 tataaacagt tgcaactaat agtaaatagg tgtaaactat tttcgtaccg aaataccata
     1741 aacacaaaca gaaagaggtc ataccatgaa agaaccatta ttgttttccg ttgacttagg
     1801 gaataaatca gtaaaagaaa tgagtacaca cgtttctcca ctctcacttc cctctcgtat
     1861 tttgaacaaa aatatcatta attcaggacg tttttctaat ataggttcct cattaaaacg
     1921 atcactaaac gtttctgaat atagcctagt aaacactact gaaagctatt actttgggaa
     1981 ggatatacac gagctaggaa aagatgacta cgtgatagaa tcactaggtt ttgggatcag
     2041 cagatacaag caggaaacct accaaaatat cctatctttc tctttagcta acctagcacg
     2101 tttagaagat caatctgatg acgttttaac cgctaatcta gttttaggtc tgccttcatc
     2161 cgactatacc gaaacaatcg tcaaatacgt gatgcaacaa gccacacgac aacattcagt
     2221 tgaagttgac ggtaaagctt ataatataaa cgtagatacc gtttgggtac tgccacagcc
     2281 gattggaaca ctatataacc tcctacttgc tgatgatggt agtgtacaag atgaaacatt
     2341 gatcagtcag aagatcggtt ttgttgatat tggtggtggc acgaaacttt ttgatgttgt
     2401 ccaaaatttt gaaattgacg aaaacaaccg tgaacaaaag gataccggta tttttacttt
     2461 atataatcgt atcgtaaata tgcttgacct tccgatcagt cctaatgctt tccaacttga
     2521 aaagattatc cgagaaggca aagaaaaagg cgtatatcta tttaaaccaa gcggaaatga
     2581 aagttttgat atttcagaag tagttgaaag agaaataaaa acatacacca atatggttgt
     2641 tgcagaagtt ttagctacat tcaaaaactt cacaacctta gataaagttg tctttactgg
     2701 tggtggggct tctattatcg acaaacaact tgtaaaagat aaaattccgc ataccttgtt
     2761 tgttgaagac agtgaatttt ctaatgtaaa aggcttttac aagtttggtt taactcaata
     2821 aaacagcgaa ggagatcaac atgaataagg gaaaatcaga gcgttatgta gttataatca
     2881 atagagaaaa ggttagcgag ttacctttag cagagtttct tgaatctgaa tatgaaacac
     2941 taggcgttaa atactctcag acgatcaaaa aagctttgaa tctatatcgg cgtgtaaaga
     3001 acaatgaagt tacaattatt gaagcaggca acgatagaaa cgatagcaaa gtagctgaag
     3061 taaagaataa accaactgat cataacccta ttcaacaagc gcatccttca acggttacat
     3121 ccgatcattc agcaaagaaa gaaaagccaa tgctaaaagg gttcaatgca ccaagtaaca
     3181 aagacttact ttaaaaataa aaaggcgagg ttacaatacc tagccttttt attatgtaca
     3241 aatacaaaca taaacatata cgtatacatg tatataaaca tgtatacgta tatatccata
     3301 aacacgtata cgaaatttag ttaagctata cctaaccgct tattccaata aaaaaagtga
     3361 tcagctttaa actgatcact caccaaaaac ttcatcatac aagttatcta tcatttcacc
     3421 aagtaaagct gaatctgatc gaattcctaa cttttcgctg attttagata gtttctttct
     3481 ttgtgatggt tgaagcgtga attgataatt tttcttcttt tcagaactat cactttttag
     3541 tttagctacc aaagaacttg ttgtttcttt aggtgtttgt ttaggttctt caactgcttc
     3601 aaccacttca ggaacttctt ctacttcagc ttcagcgaat tttttaaatc tattttcttt
     3661 cacttcaatc actccttatt cttgtgccgt atcaattatt ttgttgtata gcgtaaacgt
     3721 gctttcaaag aaatccttat tcttacgata gatattctta tccttagcga actcagcaat
     3781 attcttacct tctaagctag ttgcattaag caattccttc tcttgtacat atccaagata
     3841 attagataac gttggtacca attctaggaa ctcacgactt gaattagtat tatgtttgat
     3901 cctgctaccg atcgtagcgt atttagtttc catcagtgtt tcaccagtct taatatcaat
     3961 caactgtttt gctagtgcat caatatcttt accaagctta agcaaagctt caaaaccatc
     4021 tttagatgga tcagaaatgc ctacgacaag atcagataca gcccaagcat tttgagctag
     4081 tagacctgca tcattatgag tatcaatcag aattagatca tacttctctt taagctgttc
     4141 atagttaaca acgatccatc taaacagtag ggttctgttg ttaggcatat cacgcatttg
     4201 catctcaact tgttttaaat cttcacctgc tactaaaata tccatgttct catttatagc
     4261 taaaggttca gctgatccat gaagatataa atgattgatt gtattctctc ttttcgcttt
     4321 actgaaagca tcaggaaatc gttttgatag gttttgcgac ttatcttcat caatatacag
     4381 caccttcata cctaaatttt cgacagcata tccacctaga ttgtagattg ttgttgtttt
     4441 acctgttcca cctttttcaa tgtttccagt aatgattgac aacttctttt ccatgattaa
     4501 acctccctta ttaatctttt gtaactccat tatacactat aaacacgttt atgtatatat
     4561 ttatatacat aaacgtgttt atatttatat ttgcttttat attctacaac ataaacatat
     4621 atatgtatat gtttatgttt atgtatatat tcaaatatat aactattgca tttgtcatac
     4681 aatcgtagta caatgtaatt gaaaaggagt tgataaacta tgccagtaat aagtattcgt
     4741 gtatctaaag aagaaaaaga ttttcttgaa aaagttgctg aattcaacgg agataacatt
     4801 tctgaattta tgcgtagtaa ttccctgcaa acagcagaaa cgttagtaga ctttgccacc
     4861 tacaagcaac taatgaaaga gcatgaggaa aacgatcaaa gtatttccca cgaagatatg
     4921 ctaaaagagt taggcttata agatgactta ccaagtaaga tatgaacgtg aagctcaaaa
     4981 agcgctaaaa aaaatggata agtttcaggc aaaggttatc ctaaactgga tcgagaaaaa
     5041 tcttgtaggc actgatgatc caagaaagca tggtaaaggt ctaacagcta ataaatcagg
     5101 ctactggcga tatagggtag gtgcataccg tataatagct gatattagcg agcatgaagt
     5161 gacaattctt atattgtcta ttggtcacag aagtgaagtt tataagtaac agacaagctg
     5221 ataaggcttg tctgtttttt tgttaaccca acaaaatcac tgtttacgat cccactttac
     5281 ctaaaaaaga acaatatcct cttgatatta tacacaccag tgtgtataat ataagtgtaa
     5341 acaagctaat taactttcaa aaggagcgat tttttatggg atggtatggt gattattcat
     5401 cagcaaatga agtgatcgaa gaaaagaaag ggtacgccaa aaatggtttg gaaatcgtag
     5461 gtaccaaagt aacgaagacc tatggaatta tccttttcaa agatgcagaa ggaacctacg
     5521 atattgacag cttcatcttt cgagatgaaa tgtacaaacc aatctacttc ttaaatgaca
     5581 gtcctgctta ctttaagaaa attccgaaaa aatggttgaa attctttgtc ggtgatcaag
     5641 aaaaaatggt taagctacaa ctagatagaa ttaaagaaga aagcaataaa ccaaaacttg
     5701 atgacttatt agttgcaggt aaaacttact caatctggga tggatcgtat gtcgctgtct
     5761 atcagtataa actcaaacgt agctttattt ttcgactaca agatggatca ctcactcggt
     5821 tcactcgact atcagcagga gatgttaagg aagtaaccta aaacttaaat acataaacat
     5881 aaacgtgtat acgttt
//
LOCUS       DAPBBG010000099         5877 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPBBG010000099 DAPBBG010000000
VERSION     DAPBBG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.103"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            641..1375
                     /locus_tag="RD477_002696"
     CDS             641..1375
                     /locus_tag="RD477_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7634999.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="RD477_002697"
     CDS             1391..2560
                     /locus_tag="RD477_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7635000.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="RD477_002698"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="RD477_002698"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDT7635001.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="RD477_002699"
     CDS             3650..4576
                     /locus_tag="RD477_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDT7635002.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="RD477_002700"
     CDS             4595..5845
                     /locus_tag="RD477_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7635003.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1968 a          954 c         1203 g         1752 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaatttctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DAPBBG010000100         5340 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPBBG010000100 DAPBBG010000000
VERSION     DAPBBG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.105"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2616)
                     /locus_tag="RD477_002701"
     CDS             complement(<1..2616)
                     /locus_tag="RD477_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDT7635004.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISD"
     gene            2762..3337
                     /locus_tag="RD477_002702"
     CDS             2762..3337
                     /locus_tag="RD477_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7635005.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3551..4246
                     /gene="vanR"
                     /locus_tag="RD477_002703"
     CDS             3551..4246
                     /gene="vanR"
                     /locus_tag="RD477_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDT7635006.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4224..>5340
                     /locus_tag="RD477_002704"
     CDS             4224..>5340
                     /locus_tag="RD477_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7635007.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVEL"
BASE COUNT         1665 a         1009 c         1019 g         1647 t
ORIGIN      
        1 atccgatata taattcaaaa taaagatcgt tttttctatt cggcccatct cacgtaaggc
       61 tgtagctaag ctgttttgtc ttgaatagga acctagcttc cccataataa gggatgctga
      121 aactgttccc tcccttatag aatgagctaa tcgcaaaaca tcctcataat tttctttaat
      181 gacctttgta tttatttgtc cacgtaaaat ggcttctagt tttggatact cacttgcttt
      241 atctatcgta aataattttg agtccgataa atcccttatt cttggggcaa atttaaatcc
      301 taataaatga gtcagtccga atatttggtc agtgtaaccg gcagtgtctg tataatgttc
      361 ctctatgttt agatccgtct catgatgtaa caaaccatcc aaaacatgaa tcgcatctct
      421 tgaattagta tgaataatct ttgtgtagta agaagagaat tgatcacttg taaatcggta
      481 gatggtggct ccttttccag ttccataatg tggatttgca tctgcatgta gtgatgaaac
      541 acctagctgc attctcatac catctgacga agatgttgta ccgtcgcccc aatagaaagg
      601 caattgtaat ttatgatgaa agtttactaa tatggcttgg gctttattca tggcatcttc
      661 atacatgcgc cattgagata cattggctag ttgcttatat gtaagtccgg gtgtggcttc
      721 ggccatcttg ctcaagccaa tattcattcc cattcctaaa agggcagcca tgataatgat
      781 tgtttcttcc ttatctggtt ttcgattatt ggaagcatga gtgaattgct catgaaatcc
      841 tgttatatgg gccacatcca tgagtaaatc agttaatttt attcttggta gcatctgata
      901 aaggcttgca ctaaattttt ttgcttcttc tggaacatct ttttctaagc gtgcaagtga
      961 tagctttcct ttttcaagag aaaccccatc taacttattg gaattggcag ctaaccactt
     1021 taacctttca ttaaagctgc tggttctctc cgttatataa tcttcgaatg ataaactaac
     1081 tgataatctc gtattcccct tcgattgatt ccatgtatct tccgaaaaca aatattcctc
     1141 aaaatcccta tattgtctgc tgccaacaat ggaaacatct cctgcccgaa catgctcccg
     1201 aagttctgtt aaaacagcca tttcatagta atgacgatta attgttgtac catcatcctc
     1261 gtataaatgt cttttccatc gttttgaaat aaaatccaca ggtgagtcat caggcacttt
     1321 tcgctttcca gattcgttca ttcctcggat aatctcaaca gcttgtaaaa gtggctcatt
     1381 tgcctttgta gaatgaaatt ccaatactct taatagcgtt ggcgtatatt ttcttagtga
     1441 ataaaaccgt ttttgcagta agtctaaata atcatagtcg gcaggacgtg caagttcctg
     1501 agcctcttct actgaagaga caaaggtatt ccattcaata accgattcta aaaccttaaa
     1561 aacgtctaat ttttcctctc ttgctttaat taatgcttgt ccgatgttcg taaagtgtat
     1621 aactttctca tttagctttt taccgttttg tttctggatt tcctcttgag ccttacgacc
     1681 ttttgataac aaactaagta tttgcctatc atgaatttca aacgctttat ccgttagctc
     1741 ctgagtaagt tgtaataaat agatggttaa tatcgaataa cgtttatttt cttgaaagtc
     1801 acggaatgca tacggctcgt atcttgagcc taagcgagac agctgcaaca ggcggttacg
     1861 gtgcaaatga ctaatttgca ctgtttctaa atccattcct cgtatgtatt cgagtcgttc
     1921 tattattttt agaaaagttt cgggtgaagg atgacccggt ggctctttta accaacccaa
     1981 tatcgtttta ttggattcgg atggatgctg cgaggtaata atcccttcaa gcttttcttt
     2041 ttgctcattt gttagagatt tactaaccgt attaaatagc ttcttttcag ccattgccct
     2101 tgcttcccac accattcttt caagtgtagt gatagcaggc agtataattt tgttttttct
     2161 tagaaaatct atgcattcat gcagtagatg aatggcatca ccattttcca aagctaattg
     2221 atgaaggtac ttaaatgtca ttcgatattc actcagggta aaagttacaa agtcgtattc
     2281 acttcgaatt tctttcaaat gatcccaaag tgtattttcc ctttgaggat aatgatcaag
     2341 cgaggatgga ctaacaccaa tctgtttcga tatatattgt atgaccgaat ctgggatgct
     2401 tttgatatga gtgtatggcc aaccgggata ccgaagaaca gctaattgaa cagcaaatcc
     2461 taaacggttt tcttccctcc ttcgcttatt aactatttct aaatcccgtt tggaaaaagt
     2521 gaagtaggtc cccagtatcc attcatcttc agggatttgc ataaaagcct gtctctgttc
     2581 cggtgtaagc aattctctac ctctcgcaat tttcattcag tatcattcca tttctgtatt
     2641 ttcaatttat tagttcaatt atatatcaat agagtgtact ctattgatac aaatgtagta
     2701 gactgataaa atcatagtta agagcgtctc ataagacttg tctcaaaaat gaggtgatat
     2761 tttgcggaaa atcggttata ttcgtgtcag ttcgactaac cagaatcctt caagacaatt
     2821 tcagcagttg aacgagatcg gaatggatat tatatatgaa gagaaagttt caggagcaac
     2881 aaaggatcgc gagcaacttc aaaaagtgtt agacgattta caggaagatg acatcattta
     2941 tgttacagac ttaactcgaa tcactcgtag tacacaagat ctatttgaat taatcgataa
     3001 catacgagat aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga
     3061 agataatcca tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg
     3121 agatcttatt cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt
     3181 taaaggtcga ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct
     3241 atataaagaa ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc
     3301 ttcattatac aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg
     3361 caatattttt aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga
     3421 aaagcccttt gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta
     3481 aatgcttata aattcggccc tataatctga taaattatta agggcaaact tatgtgaaag
     3541 ggtgataact atgagcgata aaatacttat tgtggatgat gaacatgaaa ttgccgattt
     3601 ggttgaatta tacttaaaaa acgagaatta tacggttttc aaatactata ccgccaaaga
     3661 agcattggaa tgtatagaca agtctgagat tgaccttgcc atattggaca tcatgcttcc
     3721 cggcacaagc ggccttacta tctgtcaaaa aataagggac aagcacacct atccgattat
     3781 catgctgacc gggaaagata cagaggtaga taaaattaca gggttaacaa tcggcgcgga
     3841 tgattatata acgaagccct ttcgcccact ggagttaatt gctcgggtaa aggcccagtt
     3901 gcgccgatac aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc
     3961 cggccttgtc attaatgtta acacccatga gtgttatctg aacgagaagc agttatccct
     4021 tactcccacc gagttttcaa tactgcgaat cctctgtgaa aacaagggga atgtggttag
     4081 ctccgagctg ctatttcatg agatatgggg cgacgaatat ttcagcaaga gcaacaacac
     4141 catcaccgtg catatccggc atttgcgcga aaaaatgaac gacaccattg ataatccgaa
     4201 atatataaaa acggtatggg gggttggtta taaaattgaa aaataaaaaa aacgactatt
     4261 ccaaactaga acgaaaactt tacatgtata tcgttgcaat tgttgtggta gcaattgtat
     4321 tcgtgttgta tattcgttca atgatccgag ggaaacttgg ggattggatc ttaagtattt
     4381 tggaaaacaa atatgactta aatcacctgg acgcgatgaa attatatcaa tattccatac
     4441 ggaacaatat agatatcttt atttatgtgg cgattgtcat tagtattctt attctatgtc
     4501 gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat aaataccggc attgatgtac
     4561 ttattcagaa cgaagataaa caaattgagc tttctgcgga aatggatgtt atggaacaaa
     4621 agctcaacac attaaaacgg actctggaaa agcgagagca ggatgcaaag ctggccgaac
     4681 aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat taaaacgccc cttacatcca
     4741 ttatcggtta tttgagcctg cttgacgagg ctccagacat gccggtagat caaaaggcaa
     4801 agtatgtgca tatcacgttg gacaaagcgt atcgactcga acagctaatc gacgagtttt
     4861 ttgagattac acggtataac ctacaaacga taacgctaac aaaaacgcac atagacctat
     4921 actatatgct ggtgcagatg accgatgaat tttatcctca gctttccgca catggaaaac
     4981 aggcggttat tcacgccccc gaggatctga ccgtgtccgg cgaccctgat aaactcgcga
     5041 gagtctttaa caacattttg aaaaacgccg ctgcatacag tgaggataac agcatcattg
     5101 acattaccgc gggcctctcc ggggatgtgg tgtcaatcga attcaagaac actggaagca
     5161 tcccaaaaga taagctagct gccatatttg aaaagttcta taggctggac aatgctcgtt
     5221 cttccgatac gggtggcgcg ggacttggat tggcgattgc aaaagaaatt attgttcagc
     5281 atggagggca gatttacgcg gaaagcaatg ataactatac gacgtttagg gtagagcttc
//
LOCUS       DAPBBG010000101         5327 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPBBG010000101 DAPBBG010000000
VERSION     DAPBBG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.106"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(87..1235)
                     /gene="nagA"
                     /locus_tag="RD477_002705"
     CDS             complement(87..1235)
                     /gene="nagA"
                     /locus_tag="RD477_002705"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDT7635008.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1311..2147)
                     /locus_tag="RD477_002706"
     CDS             complement(1311..2147)
                     /locus_tag="RD477_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7635009.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2331..3548)
                     /locus_tag="RD477_002707"
     CDS             complement(2331..3548)
                     /locus_tag="RD477_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7635010.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3697..3876)
                     /locus_tag="RD477_002708"
     CDS             complement(3697..3876)
                     /locus_tag="RD477_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635011.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3931..4014
                     /locus_tag="RD477_002709"
     tRNA            3931..4014
                     /locus_tag="RD477_002709"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3965..3967,aa:Leu,seq:gag)
     gene            complement(4254..5252)
                     /locus_tag="RD477_002710"
     CDS             complement(4254..5252)
                     /locus_tag="RD477_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635012.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1660 a         1082 c          841 g         1744 t
ORIGIN      
        1 ttatgtaaag tgatttttac tagaacacaa aaaaaacctt aaaaccaaac ttaagaagtt
       61 tagttttaag gttaacttaa attatattat gcgtggaaac gttccacgcc atctagataa
      121 gttgcttcca atgtcatatc agggttcaat acgataaagt ctgcatcgcg tccttgagcg
      181 atttttccac atgtatcatc gattttacag ctgattgctg gaatcagact agccatcatg
      241 actgcttgtt ctggtgttgc taatccccag tctacaacat ttttaattgc ttctttcaat
      301 ttcaatatac ttccagctaa gttgccagat tctagacgtg ctgttccttc ttttactact
      361 actggaaatt cgcctaaatt gtaatttcca tcaggcatcc caccggccat catacagtct
      421 gtaattaatg cgacatgatc atgtcctgct ttttccatta ggattcccgc agcatttgga
      481 tgtacatgat ggccatcaca aatcaattca gaaaagacat gttctaaaga taataacgca
      541 cccaccatac ctggttcgcg atgattcaat ccacgcatac cattgaaagc atggacaaat
      601 acacttgcgc ctgcttcaac agctttttgc gcttcttcta gtgtagcatt actgtgtcct
      661 agagaaacaa ctactccttc ttcagtcact tgtttgacaa actcttctac accttgacgt
      721 tccggtgcta aagcaatttt tttgattaaa ccacctgaag cttcttgcca ttcatggaaa
      781 gtatccaaat caggatcccc gaaataactt gggttttgtg cccctttgtg ttcttctgtg
      841 aagaaaggac cttcaaaata gattcctttg attttagctc ctggtgcttc ctgatacatt
      901 ttgccgattg tctcagctac atcttttaaa cgttctttgc ttgacgtcaa tgttgttggt
      961 aaaaatgaag tcactccaca agataacaga ccttcagaca tcactttgat gccttctgca
     1021 tcattatcca ttacatcatg attcatatag ccgtgaatat gtgtatctac caaaccagga
     1081 gcaatccatt tgccgctttg gtcaatgatg ttgacttctc cctcaggtag ttctttggtg
     1141 tattcaccaa aaagtccatc ggtaatttca agatagcctg catgtttgac acctgacgct
     1201 aaaaagaatt tgtcagcata gataaaagtt ctcattctat tctctccctt tgtttttcta
     1261 cctctaaagt actcttattt agagaagaag tcaagaatgg tctacaccat ttacaaattt
     1321 tttgaaattt gtttcacagc ttgtgctaag ccttcataga atgctgctct ttctggttct
     1381 tctttacgct tgtatattaa gcttttaagc acaaataaat cattgaggta atagtagcta
     1441 ttcttctctt gagcccaata gattccttga ttcacctgtt ctaacgcttc gtcgatttca
     1501 ttttgatgga ataagaaatt tccataatta taaaaaattt gcgaaaattc actttttgat
     1561 cgttcatcag ctgattgatg cagcagttgg atgcttcgac tcaaatagta gcgtgcttca
     1621 gccgtttttc ctgccacacc atatattttt ccgatacaac taatcagttg tatttctgtg
     1681 tcagaaacat ggattctttc tttttggcta gagtagctta atgcttcttt caaataataa
     1741 agggactgag agatattttg acgcaacgtg aattcgcaaa caccgagata ataataatag
     1801 cgttgaaagt cagttgcttg atacagattt tcagaaatat cagaagattt tagtacttgt
     1861 tctaacttcc gataatcacg tttttggaaa caactatcca acagttcaaa taatttatcg
     1921 tttgtccgag tagtttttcg agagagattc atcacatgat caatcgttac ccctagccgt
     1981 tgacaaatct gcatcatcac caatacattc ggcagttcct cattattttc gatccgactt
     2041 aatacacttt gtgaacaaat atcatgcgaa agcatttttt gcgtcatttt ttgttctttt
     2101 cgaatctctt taatgatcgt tccaaaggac tcgattaacg tttccataag tatcctcccc
     2161 aaaatatgcg tattcgcatt ctattcgtat tttactttag gaagtcgcaa acttcaagaa
     2221 aagacttgca actaaataat atactttagt tatttaagaa aactccacta gaaagagagc
     2281 ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg gtttttaaaa tcatttttgg
     2341 cgataggttg ctaggaattc tctcattttc ttagctgttt gtttcaaatc atccatttta
     2401 ggaagataaa cgattcgaaa atgatctggc tggttccaat taaatccacc accatgtaca
     2461 agtagaatat gatgttcatg taaaaagtcc aaaacgaatt tttcatcatc caaaatattg
     2521 aatcgctttg tatcaatttt tgggaatata taaaaagcag cttttggttt tacggctgaa
     2581 agaccaggaa tatcattgat tgcattatag atatattctc tttgttcata gatccttccg
     2641 cctggcagga gcagctcatc gacactttga taaccgccta gcgcggtttg gataatttgc
     2701 tgagacaata cattggaaca caaacgcatg gaggacaaca tattcagccc ttctatatag
     2761 cctttgacgt gtgacttatc tccacttagg accatccagc ctactcgaaa tcctgctaca
     2821 cgatgagatt tggatagacc gttcagcgta acaacgaaca aatcaggcgc taatgtagca
     2881 atcggaatat gttctagacc atccatcact aagcgatcat agatttcgtc ggaataaata
     2941 atcaggttat tttgtcgagc tacttcaacg atttcttcta ataattcttt tggataaagt
     3001 gccccagtag gattattagg attaatgata acaatagctt ttgtccgact cgttattttt
     3061 gatttgatat catcaatatc aggataccat tctgcctctt catcgcaaat ataatgaaca
     3121 ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg gatagtccgg cattggaacc
     3181 agtacttcat ctccattgtc cagcaacccc tgcatacaca tcgtgatcaa ttcgctcact
     3241 ccgttgccgg tgtagatatc attgatcgtc acattcggaa aatgtttcag ttggcaatat
     3301 tgctcaattg cttttctagc agaaaagatc cccttggaat ctgagtagcc ttctgagtca
     3361 cgggcattca tgatcaaatc acgaatgact tcgtttggtg cttcaaatcc aaaaggtgcg
     3421 ggatttcccg tgttcaattt taaaatacta atgccctctt cttgcatccg ctccgcttct
     3481 tctagaacgg gaccgcgaac atcgtaactg acaccttcta atttgttgga tttttcaaat
     3541 tctctcatca cttagccaat ccctcatttc aaaaagttag aactttccaa attttttgtt
     3601 atttactatt tcaatatcat actccatcct agaaaatttt gccacttatt taggaaaaac
     3661 gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa ttgtcatgat agtcattcca
     3721 tagattcgct gcccaatcca ttgcaattcc acaaatgatt cctagcataa aaccaagcaa
     3781 aaaacgtccg aatgcaagtc caatcaatac acctaagatc attcccagtc ctgaaaattc
     3841 tccgtcatat ttctccatct taataagtcc tcccattttt gagcttgaca actatgtaaa
     3901 acctcgctat cattagtgtg ttattattac gcagatgtgt tggaattggc agacaagcat
     3961 gattgagggt catgtgagcg ttagctcgtg tgggttcgag tcccatcatc tgcactacaa
     4021 ctcgtgaagt taattttgct tcacgagttt ttttgtttga acgtttcttt atgccattat
     4081 atacatatca ctgtatcctt tacaaaaaga acgtacaatc atgtaaacag cctgaggtga
     4141 gtatatgaag gtcggaaaca agctactcgt agaaacttgc aagtgttaaa acaattaatc
     4201 gaaaaacgag taaaatccct cacctaatac taaagcgagg gattttttgt atattatctg
     4261 tttatcaccg ttattataaa ggtacaaata ccaagcacgg ttaatccaaa ttgcataaat
     4321 tcattttttt gtacactcat gcttttcatt agctctttaa ctgccattag tactaagatg
     4381 gcgataacaa cataaagtat atagtacgtc cgcattccgg aagcccatac tgtgggagaa
     4441 aatcccataa ttaaccttga acaaaatccc attatcatca aaataactaa aaaaatagaa
     4501 gttttcttat tgtcaaaact taaataaatt ccaacaatta aacatagtaa taatgccaag
     4561 actactaaag tagctatcca agtacctgga taaaaaagtg acagtttcgt tcctaactct
     4621 gtaaagagat tattcaggtt actactactc caaatcattg cccttttatt tccattgacg
     4681 taagtaaagc tttgacccat tgtattacca aaataaataa tcaggtttaa gaaaaacggt
     4741 aatgcagtca atatacgcac ataatagttt tgacatttcc tatatgttag aaggaaaatt
     4801 aagaaaaata gcaataaaaa taagatattc atgtctaaaa aaaacggctt tccaaatgat
     4861 gaaataccta aatctaactt attaagaaag ttgaaatttt tatattcagg aaaccattta
     4921 ttaatttcat gtgcagcacg taatgcattc cctggagttg tcaaagagaa aattaattct
     4981 gctaagctta ttattgaaaa tactgataac ttataattat atcgtccatt gacaatcaaa
     5041 tataaactaa ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc ttgattagtt
     5101 aaaaatatca ataaaggaat acttacccaa tatatttttc tatttatttc ttctcctctt
     5161 aagagttgaa aaaacggata aaaagttagt aaactaaatg ctactggcca ctgatagttc
     5221 aaggtggttg ctctccaacc ggtttccccc attactgtca atggtattaa acagtatata
     5281 gagcaaacca ataataaatt tttaacattt attttctcaa aaacata
//
LOCUS       DAPBBG010000102         5247 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPBBG010000102 DAPBBG010000000
VERSION     DAPBBG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.107"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            365..2938
                     /locus_tag="RD477_002711"
     CDS             365..2938
                     /locus_tag="RD477_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635013.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTRNVVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVMNTIVVGLMILSLMFVGYKMITGKGTIDLKSVGTNIVMSVALI
                     LLMPTMISSGIQFSKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYFFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVE
                     DRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLLK
                     K"
     gene            2953..3171
                     /locus_tag="RD477_002712"
     CDS             2953..3171
                     /locus_tag="RD477_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635014.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            4888..5202
                     /locus_tag="RD477_002713"
     CDS             4888..5202
                     /locus_tag="RD477_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635015.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT         2050 a          701 c         1002 g         1494 t
ORIGIN      
        1 tataaacgaa aaactgaatc tcggtgaaaa aattgatgaa atgaagttca tcaacaccac
       61 agctagaaac cattgggagg acatagctaa gcaagaaaaa gctagtagac aggatatgct
      121 agaggcactg aaaaaaatag gttagaaagg attaataata tgattgaaaa acggttaata
      181 tatcaaattt attgtcttga tcataacgca gatactcttg agttaatcga gagtatctgt
      241 ttcaatacta tagtcctcga tttaaaggct aaaaacgatt gttacgtcac tcacatcata
      301 attgacagaa aaacggctca taagctctct gagagcctaa gaaaatgggc tgaaggagaa
      361 aactatgaaa gcaattaaag aaaaaatcgt tagtagaaag ataaactggt tagcactcag
      421 tgtgcttgtt ttaatgatta gcgttgtttt tacaaggaat gttgttgaag ctagcatttt
      481 caattggttt gatgacacaa aaagtcaaac agagtttttg aataattctc tttatagacc
      541 ttacctgaaa aaatacaatg gagatttcct tagtcagttc gggatttggc ttggatgggc
      601 tgtggtaaaa ggcatgttta ctgtgactga ttcgattcaa aatatgattc cagatgtgct
      661 ggatttgttc aatttcattg aaagtacagg gctaaataac gtttatcaat cagtcatgaa
      721 tacaattgtc gttggattaa tgatcctttc attgatgttt gttggctaca agatgattac
      781 tggaaaagga acaatagatt taaaatctgt tggaacgaat atagtgatgt ctgtagcatt
      841 aattctttta atgccaacga tgatttctag cggtattcaa ttttccaaaa tattttacaa
      901 tgatgctact acgattacaa attcagatga cggagtcgct tggtcattga taaaacaagg
      961 agtaacagat ttggcatata ttaataaaac agatcagtac tcaagcattg ataaaacgga
     1021 ggatcgaaat aagttaacac gaaaaaattt tcaacagacg gatctcacac aagtgttaac
     1081 ggataaagtg attgataaat tggaaaaaga aaatccagct gcggataatc ttcgctatga
     1141 attagttgaa aattctaata atgaatttgt tgctacaaag ttctctgata acttcttatc
     1201 aacattttct gatagtttga aatcaggcta ttatcgctat caagcaaatc tttgggggat
     1261 ttcgattgga ttgacagcgt tagcaatcgc ctattttttc agcgcgtttg tgatcattac
     1321 agcaatatta gaacttgcat ttaaacgtgt gttaggtgtt cttgtttttg cgacagatat
     1381 tgaaacagga caacgttcaa aagttgtttt atccgatatt ttacaatgtt acttaacagt
     1441 tggatttcaa gggtttggac tatctatgtt tgctatgttt atcaattttt taaatgctgg
     1501 acaaggtatc tccacaaata tttttataaa aacaattgct tatatctgtg ctgtgtttgt
     1561 gctgataaaa ggcagtggaa cagtcatgag atactttggt gttgatattg gactcaaaga
     1621 aggctatggg caacttgctt ctgcttttgg tatgggcgca atgctgttta gaaaaggatc
     1681 aactggattt aatcgtgcta aaggaagcgg aaatggaaat ggatcagaca gtggagaagg
     1741 agaagatcga aaaccagaaa agaatttcgg cgaaactcta tcaaaaaaag ctggcaaaac
     1801 tggaagagct ttgggatatg cccatgaacg tggattatca ggtttagctt cagatggagc
     1861 tacaatggct agtgaaagag caataaaacc atttaagagt atgcgtgata tggctaatga
     1921 taccaagaat aagttcaaag aaggtttaga tgatggtaca gtgtccgcta ttaataaaaa
     1981 tagcaaacct atgttagcta aaaataaaga agatgaaact ggaaaatatg ctgattccat
     2041 gccaacacgt ctttcggata gaaaagatgg tgctaaggtt gaaaatgccg atagaattat
     2101 gtctagttct gaacgtatgc gtgaagcaat gaaaaataat gctgaatcga ataataatcc
     2161 tgtaagtgca attcagcaaa aagttcaaca agatattgaa gaacgtaaaa atgctatgca
     2221 cggtcaagct aagtctgctg aagaattaat taatcagaag cgacaagaag ctaaatacac
     2281 gcctgaagca atgaatcgtg aggaaatgtt aaggaaacgt gtagaagggc gtacaggagc
     2341 taatacggac gaaaaagaag cgttgaccaa agaaagaatc caagaagcta aaaactcaaa
     2401 taccggatta gaaaaattgg tcaaagaaaa tctacaaaat tctacaagta gtcaaggtgg
     2461 ccgatcagta gatgtccgag aaaatattca aggttcattg aatggtcatg tcggacggaa
     2521 tgttgatgta agagagaatt tacaaagctc ttcaaatggt caaggtacta agacaattga
     2581 tgttcgagaa aatgtacaaa attctacaag tagtcaaggg tttaaatcaa tggatgtaag
     2641 agagaatgta caaagttcta caagtagtca aggattcaaa acagtagatg ttcgggagaa
     2701 cgttcaaaaa gatagtggag aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga
     2761 cagaaagtca gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg atagtgaaat
     2821 ccgtgaaaat gataagggat caaaaccaag acgaagattc acttatgaag acaacgagtt
     2881 attcagagat acacttaatg atcctaatcc gcttttcgat aatctattaa aaaaataaaa
     2941 gggggaattg tcatgaaatt aattctaaag attagtacgc atgatttctt agaacttgac
     3001 gaattatgtg aatcagtaaa gaacgcaaca aatgaaaaaa tcacggtcac taattatatt
     3061 gaactgatga ttcaagaaga attaaagatg aaacatgaga taaaaaacaa cttgaaatat
     3121 tgttatctag aaaaaagtga agagataatc catgaattaa atggttttta aaaattgtat
     3181 ttgacatgaa tttacgacaa ggaattagtg gggattaagc gattattctc ttccacttta
     3241 gagaaataat gggttaaaaa gccagaatac gtagagcaat attgacaacc tataaaggag
     3301 ctattgttga aaattgaagt aacaaaaaga taaagagagg tttacatcta tcagattaaa
     3361 agtgatagta cacccatgaa acctgcaaaa gagttgaacc taatgtatat cggttcaact
     3421 ctttttattt gcaaattaaa aaaatatcac cgaagtgacg aatgaaacta tgaccaaatt
     3481 aagcaagaaa atatatcttg caaataaaat aaagtagtat aaaaagatat tgataaagcc
     3541 aatcaaaact ttgaatttca aacgttagat aatcaaacag atacaaaaaa tgaaatcagt
     3601 tctacgcgta cgattattaa aaataataaa ttttcactag aagaaaatgg aaataaaatc
     3661 aaataattag gaagaaaaaa caaagcaatt agaaaagcct gcaagcaaat ttgagtaatc
     3721 gagatgtttg tggcagaatt taactacaca ctaatgcagc cgaaagtaca caactatgca
     3781 gcagtttgac tgcgaactag tttccttcca tcataagttt tagtgactta gtgatggaag
     3841 gaaactagtt gcagcaaaac tctgcacttt cggctgcatt tttttgtaaa aagctattgc
     3901 acaacacata atcgagatcc aattttttta aggaagttta acaaaaatat gtaattctat
     3961 tgtcactatt aaagagaaag ttggagcgga atataaagaa aagagaaaag taattaaacg
     4021 caaaaaaatg tagaacttaa aaaatataaa gaggaacaac cgttaaaatt aggaatgatg
     4081 ttgtgtaaac attataattg ctatgatata aatgaagtgt taaagaaagc aaatttaaac
     4141 ccattttgtg aacagaaaag ccttagtcac tatgaccaaa actacggacg aaactatgac
     4201 caaaagtagg tactaaatta tgaccgaaaa taggtccaaa atggattttt ttatgatatc
     4261 aaaaattatg aaatgatttt gaaagaaaac cagttggcac agccaactta gccaccttcc
     4321 tgtaggaagg tgctatcagt tttcccttat gctcttttct ttcttttttg tttcatatgt
     4381 caaatgccat tttgaaatgt accatcttaa gaaatttatc cttaaaaaca attgcgttat
     4441 tggataaaaa gatgctacat tatcttaaaa aagtaatcaa atatgatgaa cttagtggct
     4501 agcatatcgt gtgattaaga tactttatag tgtaagttga aggagaaaaa atatttaaaa
     4561 tatacttccc atttcagtgg gaagtaaaat taatcttgtt gtttctacta gtaatgtatc
     4621 tttttggaaa ctatgtaacg atgtaaacaa gatttttttc agaagaaaaa acgataaaag
     4681 ttgtgataaa cgatttaaga aataggagaa aatatatcaa acgcatggta tgaaacgcac
     4741 atttaaaaag attcgactaa ctaaaaaaat aagtttacaa agcattgttg tgtattacaa
     4801 gtgatctata cactgaaaaa tgatagagaa aatctgatac gtccgcaaca atggacaatt
     4861 gaaaatgaaa gtgaggaaat tggaagaatg gctgatgaaa ataaagttgt tccgcttgaa
     4921 acactagtga taaataagga agagtttgag caactttttc ttgatttttt tgttcaatat
     4981 gacaggattt atgattttag ttcgtcacta gatttatttg aattagaggc agaaataata
     5041 gaaaacagtc gagaggagaa tggtaaaatc atcatttcag tagaaaaatt caatgaatta
     5101 ttctcagaaa catttgaaat agatacagat tattatcaac cacaagaagt tttccaatca
     5161 tttagaaatc agattattcg ctcaggtaag accgtaaact gaaaaacaat aaaagaaacg
     5221 ttgaaagaat aattattcct tcaacgt
//
LOCUS       DAPBBG010000103         5123 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPBBG010000103 DAPBBG010000000
VERSION     DAPBBG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.109"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            302..400
                     /locus_tag="RD477_002714"
                     /pseudo
     CDS             302..400
                     /locus_tag="RD477_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(623..853)
                     /locus_tag="RD477_002715"
     CDS             complement(623..853)
                     /locus_tag="RD477_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDT7635016.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1083..1901)
                     /locus_tag="RD477_002716"
     CDS             complement(1083..1901)
                     /locus_tag="RD477_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7635017.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            2062..2751
                     /locus_tag="RD477_002717"
     CDS             2062..2751
                     /locus_tag="RD477_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDT7635018.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2765..4267
                     /locus_tag="RD477_002718"
     CDS             2765..4267
                     /locus_tag="RD477_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7635019.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4280..4756
                     /locus_tag="RD477_002719"
     CDS             4280..4756
                     /locus_tag="RD477_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7635020.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4853..4963)
                     /locus_tag="RD477_002720"
                     /pseudo
     CDS             complement(4853..4963)
                     /locus_tag="RD477_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1736 a          737 c          918 g         1732 t
ORIGIN      
        1 aagtaaaaga gagtacgtag aaccatttaa aaaataaaga gaagtaagtg agtgaagatg
       61 agcagttggc ggtcttttaa aaatcccctt ttaaggggca gcgagtgtcg accccttcta
      121 gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga cttgatttta
      181 actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt tacggctatt
      241 caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta gaaaatgctg
      301 gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg aagtattttt
      361 gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag tatatttact
      421 tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa ataaaatctg
      481 tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt aacaagtgat
      541 gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa gtattttgca
      601 attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca agatgattgg
      661 gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac attcgcttag
      721 caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc tgctcatcga
      781 tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga ggatgatcca
      841 ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc tttcaagttg
      901 atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta ggaactacag
      961 ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca agtatgctct
     1021 catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta agtgggggta
     1081 gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa ttcaactgaa
     1141 tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata aagttgtgat
     1201 atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg taaaactaag
     1261 tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat ttttgcgcca
     1321 ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga taaagaaaaa
     1381 atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa atgagggtct
     1441 agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc aagactagta
     1501 tttccactag atcccactcc aaatatatat atgttttttg attgagtgat cattttaatg
     1561 gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc ttctgttaaa
     1621 ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt ttgactaaag
     1681 tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat tttttgacaa
     1741 aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact catcgtacta
     1801 taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc agacttcgtt
     1861 aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc tctacaattt
     1921 caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact ccatttttat
     1981 ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca aaaataataa
     2041 aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat ttcaaaaggt
     2101 ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga agaaagttta
     2161 atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg tgcaaattct
     2221 gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg aattattaaa
     2281 agagattatc caccagaatc tccattcatt actgccacta tgagagaagt agatgaatta
     2341 gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg acatgatgga
     2401 ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca gttatttatg
     2461 gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat agattttgta
     2521 ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc agatatagat
     2581 ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag gattcatact
     2641 ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt gggtggtgct
     2701 attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta gggggagaaa
     2761 taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa ttgctatatt
     2821 accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga gtgctataaa
     2881 tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta tgagttatgc
     2941 tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg ctgtaggact
     3001 tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc tagtatatac
     3061 tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac tagatacagg
     3121 tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc gttatagaaa
     3181 aattgaatta ccacaattct taggtttttt tggaggatca agatttattc cgattgtttc
     3241 tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac caattcaaaa
     3301 tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa cttttttata
     3361 tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt accctttgtt
     3421 ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg ctggagccca
     3481 aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta cttatggtac
     3541 tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag catgttatgc
     3601 aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt attttagcgg
     3661 ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt ttttatttgt
     3721 tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt attttgctga
     3781 tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact ttttattatt
     3841 cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt ttggtattgc
     3901 atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta aggtagctat
     3961 tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca aaaatacatc
     4021 attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa atattgagtc
     4081 tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga ttattcaaaa
     4141 acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg gcatccaagc
     4201 tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat taggaaaaga
     4261 taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag aaacttttta
     4321 tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg tcttttcaca
     4381 aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt atgctccatt
     4441 ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa aaagcaactc
     4501 aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag gagagccttt
     4561 ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg ttgatgtgga
     4621 ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt ttccggagaa
     4681 aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga attcatgtgg
     4741 aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa ataggatatt
     4801 tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt ttttatttta
     4861 ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa aagcttcgaa
     4921 atccattggc atttcgttgg aataccttga tatgattatt caaatataaa atatagagag
     4981 aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac ttaggaaata
     5041 gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac tatataccaa
     5101 atatagtaaa atgctgaaag atg
//
LOCUS       DAPBBG010000104         5070 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPBBG010000104 DAPBBG010000000
VERSION     DAPBBG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.110"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            14..175
                     /locus_tag="RD477_002721"
     CDS             14..175
                     /locus_tag="RD477_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635021.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            172..1476
                     /locus_tag="RD477_002722"
     CDS             172..1476
                     /locus_tag="RD477_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7635022.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1572..2441
                     /locus_tag="RD477_002723"
     CDS             1572..2441
                     /locus_tag="RD477_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7635023.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2553..2873)
                     /locus_tag="RD477_002724"
     CDS             complement(2553..2873)
                     /locus_tag="RD477_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDT7635024.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            2976..3422
                     /gene="dhaM"
                     /locus_tag="RD477_002725"
     CDS             2976..3422
                     /gene="dhaM"
                     /locus_tag="RD477_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDT7635025.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3397..4407
                     /gene="dhaK"
                     /locus_tag="RD477_002726"
     CDS             3397..4407
                     /gene="dhaK"
                     /locus_tag="RD477_002726"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDT7635026.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            4435..5040
                     /gene="dhaL"
                     /locus_tag="RD477_002727"
     CDS             4435..5040
                     /gene="dhaL"
                     /locus_tag="RD477_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDT7635027.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         1678 a          916 c         1131 g         1345 t
ORIGIN      
        1 tgaagatgaa aaattgaaaa aaagacaaga attacttgaa gaagtgtata cggaacgttt
       61 tggaacaaaa gaagagagag aaacggtccg tttttattct gtctccgaag agaaaaatct
      121 agatacgaca ttcattgcag atttgtataa agaaaatggg gtggaactga aatgatctca
      181 atcacccata caggtaattt attcttagat acttgtttgt ctattatgta tttttttctc
      241 gtttcttatc cgattttagg cggatttgta tggtttattg gtgtctggtg ttatgtgttt
      301 ttatacaaac ataagcaaaa agaatgggta gatgttccct tgagtgtcga accgttcatc
      361 acgatcatgg tgccagccca taatgaagaa attgtgatcg aagatacaat cgagtatttg
      421 atgacgaaat tgaattatca caactatgag gtattagtga cagacgatgg ttcaacggac
      481 caaacaccag aaatattagc tagactgatg aaaaaatacg ctaatcttcg tgtggttcgg
      541 atcgaaaaaa ataaaggaaa agcccacgcg tttaatatcg gacttgcctt tgcaaaaggg
      601 aaattgattt taagcaatga tgcagacacg gtaccggagc cagatgcact gattaggtat
      661 gtgaattatt tcattcgccc tggtgcaaga catattgctg cggtcactgc aaatatggat
      721 gtccaaaatc gtaccaaatt gattgcaaaa tctcaaacgg ttgaattctc aagtatcgtg
      781 ggcatcatta aacgaacaca gtcagctgtt ttcggtggtc tttatgcgta cagtggagca
      841 aatacgatgt atcgaaaaga agccttgatc gatgtgggcg gattcagaca agatcgagcg
      901 acagaagaca tcagtattgc ttgggatcat cagttaaatg actggttatc tgtttttgct
      961 cctgagatta tctttttcat ggaagtacct gtgacgttga agatgctata tcgacacaga
     1021 aaacgttggg caaaaggtgg aacagaagtc tggctgacga atttcaaaaa agtgatgctc
     1081 catcctttta aacatattgg aagaacgatc atcttcattg atcaaacatt gagtattgtc
     1141 tggtcaatat tcttttgtat ttccgtagtt ctttttgctg gattaatcgg gcattatgta
     1201 tatcaaggaa actacgaaca gatttatatt acttttacat tcagttttgt gttcatctgc
     1261 tttgaaatgg tcgcaggatt tttccagttg ctagcttcgc tgattgtaga tgatcgacga
     1321 agaaaattga aataccttct tttcgcacca ctatatatgc ttttgttctg gatagtgaac
     1381 gcgataacga tcgtgacgac tttcattcca gcagtcaaaa cgattttagg ttatggaagc
     1441 gggacatgga aaagcccgga acgtacaaaa aaataaaaga tgcaccgttt tttagacaaa
     1501 cagcctgttg tgtttatggt tcttccaaac atttttgctt ataataagaa attagttaaa
     1561 aagtagtaag gatgggaaaa gtgatgaaaa aaagaatgaa gccgttaatc tggccaattg
     1621 ctgtttccag tattttctta ttgggacaga cagcttttgc ggaaacttct gaacctgctc
     1681 aaacagaacc ttcaacaacc ataagctctt ctggtgctga aaacggtgcc tcggaaacag
     1741 aaatgacttc caatgtaccg acagataatt caggagaaac gatttcatct tcagaagata
     1801 catcaataaa tagttctacc tcttctgacc aaatcgatca tttgcaagac ttggaaaata
     1861 aaagaaaaga actcgaagaa gcattagaag ctgcaaaaaa caaattgaaa gaagaccaag
     1921 cagcaatcga tcaagattta gctgatctta aaaataaagt gggaaatgac cgtgcacaac
     1981 ttgagaaaaa gcttgccgaa gcgaaagcaa aagcggaagc tgtccaaaca gatcttgacg
     2041 ggaagatcca acaagcaaaa gaattcaatc agcaattaaa tcaggttgct caaacagctt
     2101 ctggaaagct aagtcaaact caagcggata tcacagcggc cagtcaagca atccacggaa
     2161 agatcagcga agcacaaaca gttgctaatg attttgaagc aaaaaaagct gacttagcag
     2221 ctagtttcca acgaataaaa gatattttgc attcggcagg agaaaaatca gtgacctctg
     2281 gaagcacgac agaaacagtt cagcaaacga cagagccatc caaatcaaca agtcagactt
     2341 tgccaaaaac aaatgaaaaa acagcttctg tcagttattc cttagcaggg gcgggtgtgt
     2401 tagtcgcagc agcttacgga atcatccgca aaaagaaata aaaaagagag gatgggaagg
     2461 tagagtacct cgaagttaga ctgaaaaatc taattcttga gtgccactac atgtcccatc
     2521 tctctttagt tattaaaaca gatgtcccct acttatctcg tggagacagc tgttccacta
     2581 catgtgacca tcagcatgcc attgtcgctg cctaatactt catagtccat ttttacgcca
     2641 atgatcgcat cagctcctaa tgcttttgca cgttcggcca tttcggcaag tgcttcttct
     2701 cttgcaccca ttagttcgtt ttcatagctt tttgagcggc cgccaaagat atttcgcaac
     2761 cccgcctcca agtctttaaa tgcatggatc ccagtaatga cctcaccaaa aacgatccct
     2821 ttataagcta ctacggcttt tccctctaca ctgttcgttg ttgttgtaat cattcgatca
     2881 ctcctctctt ttaattaaag tataacaaac ttcaagaagc actacacata ttttcccaaa
     2941 attagaaatt tattatatac ttgaattgtt cattaatgaa agcgaaacca atcgttagca
     3001 actatgtaaa agaaaggaag attcttgtga aaaaaagtat tttgcttatt tctcatagtc
     3061 aaaaagtaac agatggtatt aaagaaatga ttgagcagat gcaaaaatcc gaagaggtgc
     3121 aaattttttc tctcggcgga ataaatggcg agatcggatc agacccgatg aaaatcgtag
     3181 aagcagtcaa tgaatcaagc gatgcagcta gttattttgt ttttgcggat atcggtagcg
     3241 cggtattgaa ctcggaactg gcaaaagaca tgttggaaga agagcagcag caacgttatc
     3301 atctggtgga tgcgccttta gtggaaggag cttttgcagc agccatcact gccggtataa
     3361 cagatgatac tcagcaaatc ttaacagaag cacaaaatgc cggaaagaaa ggatgggaat
     3421 aaattgaaaa aaatcatgaa tgatccaagc aatattgtcg aagaaatgct ggaaggatta
     3481 gtaaaaagct atccagaact tgttcatcgt gtagaatctt cccgtgttgt agccaagaat
     3541 cagaaagcgg aacaagttgg tttggtttct ggtggaggaa gcggacatga accatcccat
     3601 gccgggtttg taggggaagg gatgttaagt gcagcggtgc ttggtgatgt gtttacttct
     3661 ccaactccag atcagattca gacggctatt aaggaagccg atagtggaaa aggagttttg
     3721 ctgatcgtta agaattatac aggggatgca ttgaacttcg atatggcaaa ggaattagct
     3781 gctatggacg atattgaagt agaaagtgtg atcgtagatg atgatatcgc cgttgaaaat
     3841 agtacgtata ctgctggaaa acgcggagtt gcaggtactg tccttgtaca caaaatcgtg
     3901 ggcgatgcag caagaaacgg tgcttcccta gctgagttga aagaactggg agaaaaggtc
     3961 gtacaggcaa ctaagacgat cggtctagcg cttcgagcag caactgtacc agaagtaggg
     4021 aaaccaggat tcgaactagg agaagatgag atcgagtatg gtgttggtat ccacggtgag
     4081 ccaggctatc gtcgagaaaa gatgcagcct tctaaagtac tggcaaagga acttgttact
     4141 aagattttag atgattactc gaatttacca aaagaagcag gtgtactagt caacggaatg
     4201 ggcgggacgc ctttgatgga acagttcgtg ttcatgaatg atgttttagc tctgttggaa
     4261 gaaagaggcg tcaatgtagt tttccgaaaa gtcggtaatt ttatgacctc actagatatg
     4321 caaggtcttt ctctaacact gatcgacttg acggagacac aatggaaaga ttctttagaa
     4381 agcaatgtac agacgatttc atggtaaaaa atctcaagat aaaagggtga taatatgaaa
     4441 ctaaccgttc aagaaatcca agaatggctc acgcaatttg cagaggcaat caatcagaac
     4501 aaacaatatc tcagtgatct agataccccg attggcgatg gagaccatgg aaataacatg
     4561 ggaagagggg tttcagctta tgaagaagct tttcaagccg atcatcctga aacaatcagc
     4621 gatacattca aagtattctc tatggcgatg atatcgaaag tcggtggggc ttctggtccg
     4681 ttatacggat ctgcattcat gaacatgatg aaagcgacaa aagaggtcga ttctattgat
     4741 tctcaagaaa aattaggcga agtgatcgag cagggaacag cgggtattca aagccgagga
     4801 aaggcagaag ctgacgataa aacgatgtta gatgtttggc tgccagtcac agaggcattg
     4861 aaggcaggga atttaacaaa agaaatcatt gaccaggcaa aagaacacac caaagtcttg
     4921 gtagctaaaa aaggacgagc ttcctatcta ggagagcgtg caatcggaca tatcgaccca
     4981 ggagctgctt ctagtgccat tttattttca acgcttttag atgtgatcaa tcaaccttaa
     5041 gttcaataaa actgcggtga tgctcatcgc
//
LOCUS       DAPBBG010000105         4931 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPBBG010000105 DAPBBG010000000
VERSION     DAPBBG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.111"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            85..972
                     /locus_tag="RD477_002728"
     CDS             85..972
                     /locus_tag="RD477_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDT7635028.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="RD477_002729"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="RD477_002729"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDT7635029.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="RD477_002730"
     CDS             2274..2720
                     /locus_tag="RD477_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7635030.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="RD477_002731"
     CDS             2993..4345
                     /locus_tag="RD477_002731"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDT7635031.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="RD477_002732"
     CDS             4516..4803
                     /locus_tag="RD477_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635032.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAPBBG010000106         4730 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPBBG010000106 DAPBBG010000000
VERSION     DAPBBG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.112"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(93..1055)
                     /locus_tag="RD477_002733"
     CDS             complement(93..1055)
                     /locus_tag="RD477_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7635033.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="RD477_002734"
     CDS             complement(1057..2496)
                     /locus_tag="RD477_002734"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDT7635034.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="RD477_002735"
     CDS             2681..4627
                     /locus_tag="RD477_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDT7635035.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1503 a          805 c          775 g         1647 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaggcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttgt ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctatcccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc tattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaatg tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaatcaaa tatttgagaa taacatttta tttaatcttt tcatatatca
//
LOCUS       DAPBBG010000107         4708 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPBBG010000107 DAPBBG010000000
VERSION     DAPBBG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.113"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..1155
                     /locus_tag="RD477_002736"
     CDS             82..1155
                     /locus_tag="RD477_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HDT7635036.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            1199..1468
                     /locus_tag="RD477_002737"
     CDS             1199..1468
                     /locus_tag="RD477_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635037.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1700..1996
                     /locus_tag="RD477_002738"
     CDS             1700..1996
                     /locus_tag="RD477_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635038.1"
                     /translation="MEVRIRNVDEKIVREIDRRCNELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            1998..2420
                     /locus_tag="RD477_002739"
     CDS             1998..2420
                     /locus_tag="RD477_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635039.1"
                     /translation="MTKVIDKHIYFYQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            2439..4031
                     /locus_tag="RD477_002740"
     CDS             2439..4031
                     /locus_tag="RD477_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635040.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSKYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDSKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGIEKRNLLTRKSDLRNTLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTQYLLTKEPEYQEYIQ
                     ISKALREEANSLYGESKRKSKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            4043..4378
                     /locus_tag="RD477_002741"
     CDS             4043..4378
                     /locus_tag="RD477_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635041.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPYCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFTCKSI
                     EESISLTEC"
     gene            4524..>4708
                     /locus_tag="RD477_002742"
     CDS             4524..>4708
                     /locus_tag="RD477_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635042.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1805 a          675 c          944 g         1284 t
ORIGIN      
        1 cagatcattt gaagctctat atatagctgc taaaaaatat tttacagctg ataataaata
       61 aaaaattaag gtgggttcta tgtgaaaaaa acgtatcaaa agtttttatg gattagctat
      121 atcgtcctat ttatcgttgg gtttctcgtc agtgtgtccg tgatcgtcgg aacgcttttt
      181 cttttgttct ctgacgaaga tctggtactt aaggggttaa tgagtacgtt atcgtgtgtc
      241 atgttacttt atttatgtat acagatcaac gaaattatgc cccctcattg gcaagaaaag
      301 aagaaagaat ttaaggaaga aatcgaaata aaatccaatg actggattga atacacatgg
      361 cttcctctga aaagaaatag caaacgaaag gaattttggg cattgcaatt atggccaata
      421 ggaaaacttt ttatttatat ggcttcactt atggttactt taatcggtta tttggtggtg
      481 gtctatgggg tggcctatta cttgttaagt gagccaaaac agatcgcggc gtcaaatatt
      541 ttcacggcag taatgacatt aatgtgtgtc atgtgctttt ctatttttag ttacttactc
      601 gtccaattta tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt attggactta
      661 cttatcttag gcagtatgtt aacgactcta acagatcggt tcgaacaatc tgaaaaaacg
      721 gtagagggga taaagaactt cttaacgacg attgatcaat ttataaatgg atatatgtat
      781 ttgtttgaag taggctttat tctcactttg tttggttgtg tatttataaa actcattaaa
      841 agatcaatcg aaaaagaaat ggttattgat ctaattccga cgctgagcgg ctatgagtat
      901 tacgttgatt ttggaaagta tgaggagttg atccatacgg atatcgttca cacgagtatc
      961 catgaagtaa ccgttaaggg catcagatct actttaaaag atggacagga gacgaaaaca
     1021 atcgaccgaa gaaaagaggt ctacaatttt tataaagttg aagcaagtct acgatatggc
     1081 attgggaaat tgaggttaga tgggagtaat attcaacaaa acaaaccata tgttttagga
     1141 aaagtggtga tttagaactt cctatgaaag atgaataaat aaggagaatc ttttgaaaat
     1201 gattcttctt tacagaattg tgcttatagt tggcacggag ttaaaaatct taaagaagga
     1261 gcagttattc aaagtgaaag aacatcataa tcagttacta tgtattaaaa cgggtacgat
     1321 gaacattcaa taccgaacag aatatttaga attaacgaat caaactgatt ttttgtcgat
     1381 tcgagagcaa cataagcaag aaaaattaat ggctacttat agccaattga ttgatgagtg
     1441 ggaacaattc aaggaatcta aaacataaaa ttccatttaa acaccttatt ttttaaagag
     1501 cataaggaaa cgtgggaaca ctttttaaaa aagtgttcca ataggctgtg cctattacgt
     1561 ttctcaaaaa taattgtggg aacaaaatat ttaaaaatag gatagtgaaa ggatagcgaa
     1621 ggatagtaaa aggatagaag taggatagcg aaaaggatag gctccttttc gctatccttt
     1681 ttaaattgga ggaattaata tggaagtaag aataagaaat gtagatgaga aaatcgtaag
     1741 agaaattgat cgaagatgta acgaactaag taaccgtaca ggaaataaat ggagtcgtaa
     1801 tgattatttg aaattactaa tagaaaatga ttttgatcgt cctttgatgg aatataaaaa
     1861 agaaaagttt gatcagttat tagaaaagtt ttcagaaatt caaagctata acacaaagat
     1921 attagaagaa tatgttagcc aaaataatcg tattttagaa atcttgatcg agcaaaatag
     1981 aggaagtgaa ctttgatatg acaaaggtta tagacaaaca catttacttt taccaaacac
     2041 atatcgatgt gttggatgaa attatttcta ctcatgcaga ggttaaaaac tatagtgaag
     2101 ctgttcgctt tttatgtatg aactatgatc cagaagtgga gaaaaaagta aattccaatc
     2161 aagcaaagtt gaatggaatg agtaaagaaa tttctatttt aaccgaaatg gtttcagagt
     2221 ttgcagatct tcatttgaaa gatacaggaa tactaattgg tacaaattca acggtttacc
     2281 aagaagcaaa agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa aaatattccc
     2341 ctcagttaaa gaaacctgtg aatgaatctg ttcatgccag agtagatcca tttgatccaa
     2401 cgaagattct aaaaagataa aagtagaagg ggaacatgat gaataaatcg cctgcagtta
     2461 ttctcacttg tcaatttact ttgcccaata aaaaatcctt ttcaaaatac attgattata
     2521 tgacaagaga aaaagcgctg gaagaaaagg aacgacgtac gcccgaggaa gaacaggaac
     2581 tgcgtctaat caagaatgct ttagaggatt tttatattcc tgaaggagaa actttttcgg
     2641 agagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agcgcaatcg ttgatggaac
     2701 atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt tcttatatga
     2761 cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagtgaacaa aaagaatacc
     2821 aacgcttgaa ccaagcaata aaaaaatatc aaacagagcc acacaaaaaa gcttcaggaa
     2881 accttcctgg agttttttct atggcttcag acgaagtaaa gggaagtgat ctgaaggaga
     2941 ttaagcaaat cttccaaaga gggcaacaaa aaggctcaat catttatcaa gatgttgtct
     3001 ctcacgacaa ttcctatctt gaaaaaatag gattgtacga ttccaaaaca gatactttag
     3061 atgaagaagg attaaaagca gctggaagaa aaatgatgga gacattattc gagaaagagc
     3121 aactgaatga aacaggctac tggatggcca cgattcaccg aaatactaaa catatccata
     3181 ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc atacactgag gttgaaaatg
     3241 gtgtgagtta tatcgtaccg aagggtaaga gaagacaagc aacattagac gcaatgaaaa
     3301 ccaattatgt gcatgaatta gaacgttttt cttatgaaaa agaccgaatg ataggcatag
     3361 agaaacgaaa tttattaacg cggaaaagtg atttgcgtaa cactttaaca aaagtggtaa
     3421 aagaaccagt acgttatgat cgacacgcaa tgaacttatt aaatgaagta tataaaacct
     3481 tacctgacaa acgttcggaa tggcattatg gggatgaaaa acgaaccaaa ctttcaccat
     3541 acacaagaga gaaactcgat gagttaaccc aatatttgtt aacaaaagaa cctgaatacc
     3601 aagaatatat acagatttca aaagcattga gagaagaagc aaacagtctt tatggggaaa
     3661 gcaagagaaa atcaaaggat gcagaaaaaa atgctctctt tgatttgaag aagcgcacag
     3721 gaaatgccat tctaacagaa ctgaagaaac aggatagtca aataaaacct ttaatagaaa
     3781 gtatggatca gatgcaatct atttacaatg aacgatcctt ccgaacactt aaacagttgc
     3841 caacaaatga atacaagtat agttacgaag aattcatgaa gcgaaggaaa caattccaac
     3901 aaagagttgt cacgcaacgg agtttgcgtc aattaaatcg aacgattaat gcccatgaag
     3961 aaaaatacca cgcaagtaaa gcatacgaag agcaacaacg aagaattcaa atggaacaag
     4021 aaagacagta ggaggaatag caatgaaaca atatttagat caatggaaag ttatagaagg
     4081 atcattaaga gaagaacgaa tcgagcaact tccctattgt ttagaaaaag agcatttgtt
     4141 tcagataaga gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagtcgttga
     4201 gtacccagct actggtgtgt actgttgtaa tcatgtgaag ggtgaaaaat attttatcat
     4261 tcaagaatat gagggcaagt tagcgcctta ttatactact tgggaaatga atgaagaggg
     4321 gatcaataac ttcacgtgta aaagcattga agagtctatt tcactcacag aatgttagtt
     4381 gttaagtgaa taaaagaagt aaaagagtcc tgaacgtaat tgtccaggac tctttttttc
     4441 aatgaattgt tttccgccaa ttcaatgagt ggtgcgattt ccgactacat tatatcaaaa
     4501 gaaagagaag ggaggaagaa gaaatggctt attcaactat tggagttgtt gttgaaaaaa
     4561 gcagagacaa tctcgttttt gtgacagaaa ttcaaacggg cagagctttt gtcgtcacag
     4621 acaaagcggc aaaagcttat caaagtggcg atattttaac gttaaatctg actacgaaga
     4681 cgttcgtcga tgcggcagaa gattatcc
//
LOCUS       DAPBBG010000108         4381 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPBBG010000108 DAPBBG010000000
VERSION     DAPBBG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.114"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(151..426)
                     /locus_tag="RD477_002743"
     CDS             complement(151..426)
                     /locus_tag="RD477_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDT7635043.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(398..1351)
                     /locus_tag="RD477_002744"
     CDS             complement(398..1351)
                     /locus_tag="RD477_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7635044.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1963..3456
                     /locus_tag="RD477_002745"
     CDS             1963..3456
                     /locus_tag="RD477_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDT7635045.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3591..3887
                     /locus_tag="RD477_002746"
     CDS             3591..3887
                     /locus_tag="RD477_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDT7635046.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3988..>4381
                     /locus_tag="RD477_002747"
     CDS             3988..>4381
                     /locus_tag="RD477_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635047.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRT"
BASE COUNT         1471 a          710 c          795 g         1405 t
ORIGIN      
        1 tttagtatag taaaaactag aaacaataaa atttgtgaat gttgtatacc cccaaacaat
       61 acacatattt tgaaggatta ggattaataa agctaaaaat atagtaagta tactaaatat
      121 atttactata tctagtatac ttactatatt ttaatctttt gatcgtctat tatatataat
      181 ccgttcctcg tcgtttaaag tatcaacata ggcgtccatt gcaagtttga ctatttcata
      241 cataggaata tcttttgcat aactaagatc ccgaatggtt tttagtacgg gaggatctac
      301 ttgaactggt tttctttctt tcgcagtgta ctttcgttcc cctaactcga cattaatatc
      361 atctttaatt gaaatatctt cagcaccaat tttcttctta tctttgttcg ttggtaataa
      421 tgccatttag tttaatctcc tttccaagag gtaggatatg attggccatt gagtcagcat
      481 atttaggtgt ataatcaaat ccttcaatat caccatgttg ttcatagtat tcaattcttt
      541 cttctaattc tgtaaagatg tctgcaaaaa tagcccacca tcttcgatct acataatcat
      601 ttaattggat accattctca ccatacattt ctaaccgatc cgagccttta acaatgcttt
      661 taaaaatgtt tgattcacca tacttatcaa ttgttttttg atagtttgcg tgttgagagg
      721 aacgtcgcac tgagaaaaga acaggcaaaa ttccaatgat ttgaagatga gactcttcaa
      781 aattcgtgac aatcggaact aaaactttat taataaaatc ttctgttcct tccattgcgt
      841 accgttttaa ttcttgcatt gcaataatat aatctgcagc agttaagaat gcacgaacca
      901 cattatctgt tgatggtggg cagtcgataa agatataatc gaaatttttt ctcaattctt
      961 ctaaaggttt aatgaaggcc aatgatctct tacgacgatc aggatatttt tcggacaagt
     1021 attcattaag ttcttcagat ccaggagagc cagccataat atataaatgt ggacttactt
     1081 gagtaatacc tgaaacccag tccccttttt tgattgctgc agacatggat cgatcaaagc
     1141 tagtaatttg atacgttgat ccaatgttgg aagtagtatt tctttgttgg tcataatcaa
     1201 ttagcaacac gtctttttca aaaagttgac tagaaacaat cgctaataaa tcattgtctg
     1261 tagtcttacc aatgccgcct tttctacctg taagcattac aacttttcct agtttatctg
     1321 atgttttaaa tgcagtccgt aagttttcca tagttccctc caatttccta tactaaatat
     1381 atttagtata cttcatatac ttaatatatt cgatatatct aatatactcc aaattgataa
     1441 gaattcagac tttataagga atatagtgga tatactaaat atatttgcta tattcagtat
     1501 atcagatata ttcaaaatgt caaaatatat ttgtgcgttt tgcgttgaca atttagagtg
     1561 aaaagaggta aacttaattc tatagaaacc acagataatt cactatttac aaagtagcaa
     1621 aagcagtggt actctttaaa cagaaaaatt acataaaaaa aagagacgga aataattgat
     1681 tttagcagaa tcaattattt ctgaatcaca tggttaggac atgtgttcac gctcgtctca
     1741 agaatatgta agtagctacc aactacttat attaaaagtt gatcggatac cttttccgat
     1801 tgcctttatt ctaccaaaaa aaggcgagca actcaacaga agaaagttta tttagtgtac
     1861 ttaaattaaa tattcttaaa cactgaagag agaacacata ctatggctga gattaaagct
     1921 aaactgtgtt ctctttttgt ttgagaaagg acgttacaat cgatgttgag cgaaatttat
     1981 tcagctattc ttaaaaacgg cttacgtaca tacaaatatc gaaacagcac catgaaacct
     2041 gcaggtcata ttgattccag taggagtggt gctatctttg cgtaccgtag caagcaatta
     2101 atgaatgttg ggcgtggtat ggtcattact tcggaagaag ctattttaga gaacgaaaag
     2161 aaactaacgc attggacacc taatacctat cgctttggta cttatgcgga tgattatcga
     2221 acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa tcaacacatt tgtagttgat
     2281 atcgattcaa aagagaacca tcaaggcgaa attattttag cctgtttaga tcaagtgggc
     2341 tacatgccaa cgctaatttt agaatcagat catggttatc aagtttattt tgtactaaag
     2401 acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga tcgatgtagc aaaaaaaatt
     2461 tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg gagtggatct aggatgtaac
     2521 cacttcggaa ttgctcgatt cccacaagaa aataatattg tttttaaaga aatgaactat
     2581 cagtattctt ttgctgactg gattcaatgg tcgatgaagc ttgaatcaga tcaaaaaaca
     2641 aaagaagatc gagaacgaaa actaattgtc tttcctgaga aaaaagaaat tcgtcaagta
     2701 gatgaaccgt ggttcgatat gttacttcat aaagcaaaca tcgtcggcgg agaaggacgt
     2761 cttggtcgaa ataatgtgat tttcacgctt tctttagctt attactcttc aggttatgca
     2821 ttggatactt gcgaatacaa tatgttcaca ttcaacgaac ggttacagga accgctatct
     2881 gaagcagaat tagtaaagat tgtacgtagt gcttattctg gtaattatca agcagcaagc
     2941 agagaattca ttattgaatt gtgccagcaa tgggtttcac caaccttaaa aaataatgat
     3001 ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc cacgagatca aagaaaatat
     3061 agtcataaaa atgaatggaa agaagatcta ttggcttatc taagcgaaaa aagtcttgct
     3121 agtgagcctt atttgaacct gtcaaaaaaa gaattggctt cacaactaac gatgcccaag
     3181 cgaacacttg ataagatctt aaatgagtta cgagaggaga ataaagtctt ctacaaggta
     3241 acacgaggaa gaaatggcgg aatgatcatt gcttcagtca aaacgttgtt cgcgagggtt
     3301 attcaactaa gtaaagaaaa gagactagat tattcaacaa ggatcacaga ggtatttgga
     3361 ctcaacgaga gcttagtaga agagacattt agacaattat caaccccctt gaaccctctt
     3421 aaagaagtga atctttttga agtagataca ggttgaaaaa taaaaggtgc aaattgccct
     3481 atacaatata tctatgatac ttctgtaggt gttgctccct ttgcctagct acgtgtaggt
     3541 ggtatactta taactgcgta tgcagtctta tgtatcatgg aggaagttgt atgtcgttaa
     3601 aaaattatgt ctattcacat cctgttaatg tgtttatcat tggtcgtttg ggattatctg
     3661 tggaagtgtt ctgtgagctt tatggtttta agcaggggac tttgtcttct tgggttacca
     3721 gggaaaaaac agttgcttct ttgccgattg agtttcttca tgggttgtcc ttagcttctg
     3781 gggagactat ggatcaggta tatgattgtt tgtgtgtgtt agagcaggag tatattgagt
     3841 ataaaatagc taacgagtta agaaaaagga agaagtatat ccagtagtag tttttattta
     3901 cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa
     3961 aggaaagaat ttagggaaga aatcgaaatg aaatcggacg actggattga acacacctgg
     4021 cttcctctga aaagaaatag caaacgaaag aaattttggg tattgcaatt atggccatta
     4081 ggaaaacttt gtagttacgg ggcacgactt attcttgatt taaccgttta ctcgattgga
     4141 gtatttggaa cggcttatta cttgataaat gatcccaata ggattgcagc atcagacatt
     4201 tttacagtgc taatgacgtt gatttttgtc atgctattgt ctatttttac tcaattagtc
     4261 atgcaattta ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct attcgattta
     4321 attgttgtaa atgcaattct agcgacttta ataaaacaaa ttgaacaatc tggaagaaca
     4381 a
//
LOCUS       DAPBBG010000109         4342 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPBBG010000109 DAPBBG010000000
VERSION     DAPBBG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.115"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="RD477_002748"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="RD477_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDT7635048.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="RD477_002749"
     CDS             complement(3250..3768)
                     /locus_tag="RD477_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDT7635049.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1424 a          713 c          657 g         1548 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aa
//
LOCUS       DAPBBG010000110         4196 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPBBG010000110 DAPBBG010000000
VERSION     DAPBBG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.116"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(607..1488)
                     /locus_tag="RD477_002750"
     CDS             complement(607..1488)
                     /locus_tag="RD477_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7635050.1"
                     /translation="MLYGAIEAGGTKFVCAVGTKDLEVIERIQIPTTTPIETMNNVID
                     FFKKHQEQLAAIGIGSFGPIDIHPNSATYGYITSTPKKAWQNFDFIGYLNKNLKELPI
                     VFTTDVNAAAYGEYVAGNGKGRSTIVYYTIGTGIGGGALQNGTFVEGFSHPEMGHMLV
                     RRHPVDTFKGVCPYHSDCLEGMASGPAIEQRTGMKGQELSVADPSWELEAYYIAQCVY
                     NTTLLFSPDVIIFGGGVMKQAHLRERVQEALVGLMNHYVDYPAFENYVLPPKLGDDAG
                     IIGCLALALEKSEKDIF"
     gene            complement(1510..2493)
                     /locus_tag="RD477_002751"
     CDS             complement(1510..2493)
                     /locus_tag="RD477_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7635051.1"
                     /translation="MAVKLTDVAKKAGVSPTTVSRVINNYGSLSQKTIDKVNQAMKEL
                     NYQPNSLARSLQGKNTQLIGLIFPTVANPFFGELVEQLESKLFDLGYRSILCDSANNK
                     EKERNYLNMLAANKVDGIIAGAHNLGIQEYENIELPIVSFDRYLADGTPIVSSDNFRG
                     GYLATENLYLKGARKIAILTGSEESNSPTNDRMNGYLKFMDEQQLEPQIFQFHSTARS
                     TALKNLEIKRILQNEEIDSIFCTDDLTAVLAYNLCQELDIRIPEQLKIIGYDGTKFIQ
                     NYFPHLSTIAQPITDIADILVDLLLQRIQDAEKPLESHYVLPVKLIQGKTT"
     gene            complement(2506..3984)
                     /locus_tag="RD477_002752"
     CDS             complement(2506..3984)
                     /locus_tag="RD477_002752"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDT7635052.1"
                     /translation="MKLKENWSRTDRYRPYKEWSEEHLHSLEKKINSSHWRLGYHIQP
                     VTGLLNDPNGFSYFDGKWQLFYQAYPMGSVHGLKSWYHLSSVNLIDWKNDGLKLIPDN
                     SFDSHGVYSGSALPIDNQLFLAYTGNVRDSNWGRHSFQLGAWMDKNGNITKIPTPLIS
                     SPPPGYTQEIRDPQLIAYDDGYLLIVGAQTEQKQGRILTYYSKDLQNWQFLGTLGFTE
                     EEMGFMIECPNLLFVNGKALLVFCPQGLSHEIHSYSNIYPNTYVIGQTFDFEKNELSD
                     PTHIKNIDEGFDIYATHCFSDENGRTLAISWIGLPEISYPTDEEGWAHCLSIVKELTI
                     EKEQLYQRPVKETAQLRKGKPYHFSGLETTIDAEINQYELQLEFDYSQKGTILLFADE
                     QQKHGLALSFDMIHGKMSMDRSHAGKSFAENFGTVREFSIEKKPLKLQIFVDRSVVEV
                     FVNDGQQTATARVFPTDDQTGLYIKSDREFKGKLWHLRNMNK"
BASE COUNT         1244 a          797 c          791 g         1364 t
ORIGIN      
        1 ctagaagtgg aaatgtataa aagatttatt ttatgaagtt ttcctaatat cattcactca
       61 tgatacgcag ctccgtatca taagtgatga tatttttgta atgtgttcgg gcatgctctc
      121 tacttactag taggtcaagt gattgatcat taaatagtag aaatagcatg tctaaggaga
      181 cattccacaa aatcattaac atacattaaa taaattgagc tgttgcataa tagacataag
      241 gaaatgttct tttctatatt tagaaaagct gtcaaggcga agtttaaata tcacaaaact
      301 taattggaaa agagaatcaa ctggaacttg ctagcgttgc aagttgaata aaaaaacaac
      361 caagattaga ttgtaggaac ttgtagaaga tcataatttg aagttaacaa aagaattcca
      421 cgacacctct actctgttaa gtacaggaaa cagagagtta agggcaagaa aaataggcga
      481 ggatatcggg ttgaaaggcc gattggagaa cataagttaa tagtaaataa aaagacgaag
      541 aaatcgcatc aagtggattc ttcgcctgct tattgatgag attgttgtga caacgtgctt
      601 attttattag aagatatctt tttctgattt ttctaatgct aaagctaaac acccaatgat
      661 tccagcatca tctcctaatt ttggaggtaa tacatagttt tcgaatgctg ggtagtctac
      721 atagtgattc atcaaaccaa ccaaagcttc ttggactcgt tctctcagat gtgcttgttt
      781 cattacgcct ccgccaaaga tgatgacatc aggtgaaaat agaagagttg tgttatagac
      841 acattgagcg atgtaataag cttctaattc ccaagaagga tcagcgacag acaactcttg
      901 acctttcatg cctgttcttt gttcgattgc aggtccagaa gccattcctt ccaggcaatc
      961 gctatgataa ggacaaacac ctttaaaggt atccacaggg tgtcggcgca caagcatatg
     1021 tcccatttca gggtggctga aaccttctac aaaagtgcca ttttgcaagg caccgccacc
     1081 aatacctgtt ccaatcgtgt agtaaacaat agtggaacgc ccttttccat tccccgcgac
     1141 atattctccg taagcagctg cattgacatc ggtggtaaag acaatcggta gttcttttaa
     1201 atttttattt aaataaccga taaaatcaaa attctgccaa gctttttttg gcgtagaggt
     1261 gatatatccg tatgttgctg aattgggatg aatatcaatc ggtccaaaag aacctattcc
     1321 aatcgctgcc agttgttctt ggtgtttttt gaagaaatca ataacgttat tcatcgtttc
     1381 tatcggcgtg gttgtaggga tttgtattct ttcaattact tctaaatctt tcgtacctac
     1441 tgcgcagaca aattttgttc cgccagcttc aattgctcca tataacataa aacatttgct
     1501 cctttgcagt taagtcgttt taccttgaat cagctttact ggtaaaacgt aatgtgattc
     1561 cagtggtttt tcagcatctt ggattctttg cagtagcaag tctacaagga tgtcggcaat
     1621 atccgttatt ggttgagcga tagtagacag atgcggaaaa taattctgga taaattttgt
     1681 tccgtcatat ccaatgattt tcaattgctc agggatgcgg atatcgagct cttgacacag
     1741 attgtatgcc aatacagctg taagatcatc tgtgcaaaaa atactgtcaa tctcttcatt
     1801 ttgcaaaatc cgtttgattt ctaaattttt taaagcagtg gaacgagcag ttgaatggaa
     1861 ttgaaaaatc tgtggttcta gctgctgctc atccataaat ttcaaatagc cgttcattcg
     1921 gtcatttgtg ggggagttgg attcttccga gcccgtcaaa atagcgattt tacgagctcc
     1981 ttttagatag agattttctg tcgcgagata accgcctcgg aaattatcac tgctgacgat
     2041 tggtgttccg tctgccagat agcggtcaaa agagacgatt ggcaactcaa tattttcgta
     2101 ctcctgtatc ccgagattat gagcgccggc gatgatgccg tccactttat ttgctgctaa
     2161 catgttgaga taatttcgtt ctttttcttt attattagca ctgtcacata agatagaacg
     2221 atagcctaaa tcaaataatt tactttccaa ctgttcgact aattcgccaa aaaacggatt
     2281 ggcgactgtt gggaaaatca agccgatcaa ttgggtgttt tttccttgaa gtgaacgggc
     2341 taacgaattc ggctgataat tgagttcttt cattgcttga ttcactttat cgattgtttt
     2401 ttgacttaaa gaaccataat tatttatcac tctcgagaca gtagtaggag agactcctgc
     2461 tttttttgcc acatctgtca atttaacagc cattttcttc atcctttatt tgttcatatt
     2521 ccgcagatgc cataatttcc ctttaaattc cctgtcactt tttatataca agcccgtttg
     2581 atcatctgtc ggaaagacac gggcagtcgc tgtttgctgt ccatcattta caaacacctc
     2641 tacaacggaa cgatcaacaa atatttgtaa tttcagcggt ttcttttcaa ttgagaattc
     2701 acgtacagtt ccaaaatttt cagcgaaaga tttgccggca tggctgcgat ccatactcat
     2761 tttaccatgt atcatatcaa aggatagtgc taaaccgtgt ttttgttgtt catctgcaaa
     2821 caataaaata gtacctttct gtgagtaatc gaattctaac tggagttcat attgatttat
     2881 ttccgcgtca atagtggttt ccaaaccgct aaagtgataa ggttttcctt ttcgcaactg
     2941 cgctgtttct ttcaccggtc gttgatagag ctgctctttt tcaatagtaa gttcttttac
     3001 gatgctcaag cagtgtgccc atccttcttc gtcagttgga tagctgattt caggcaaccc
     3061 aatccaactg attgccaagg ttcgtccatt ttcatcactg aaacaatgcg tcgcgtaaat
     3121 gtcaaatcct tcatctatat tttttatgtg agtagggtcg gaaagctcat ttttttcaaa
     3181 atcaaatgtt tgaccgatca cgtaagtatt cgggtaaata ttactgtatg agtggatctc
     3241 gtgagataaa ccttgtgggc agaagaccag tagtgctttg ccgttcacaa ataatagatt
     3301 tggacactcg atcataaaac ccatttcttc ctctgtaaaa cccaaagttc ctaaaaactg
     3361 ccaattttga agatctttac tgtaataagt taggattctt ccttgttttt gttcagtttg
     3421 tgctccgaca atgagcaagt aaccgtcatc ataagcaatc agctgaggat cacggatttc
     3481 ttgtgtatat cccggaggtg gggagctgat caaaggagtc ggtatcttag tgatattacc
     3541 atttttgtcc atccatgccc ctaactggaa ggaatgacgt ccccaattcg aatcacgcac
     3601 atttccggta tatgcaagga acagttgatt atcaatcggc aaagctgaac ctgagtacac
     3661 accgtgactg tcaaatgaat tatccggaat cagtttgagg ccatcgtttt tccaatctat
     3721 cagattaact gaggagagat ggtaccaaga tttcagacca tgaacagacc ccatcggata
     3781 tgcttgataa aatagctgcc atttcccgtc gaaataggaa aaaccattcg gatcgttcaa
     3841 taaaccagta actggttgta tatggtagcc taagcgccaa tgggaagaat tgattttttt
     3901 ctctaaggaa tgcaaatgtt cttcagacca ttctttatat ggacgataac gatccgttcg
     3961 tgaccaattt tctttaagtt tcatgacaaa ctccttgtaa acgtattttt ggaattataa
     4021 tatatttttt ctggtaaaat gtcaaacgaa tatcaaaatt aaatggctta taaaagctat
     4081 ttttttatat ataaaaaatt attttattta aaatttgata atcgattgac aattgtttta
     4141 aatcatggca tattgtgctt aagaaaaatt tatagttact gcttcaataa ttttgg
//
LOCUS       DAPBBG010000111         4175 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPBBG010000111 DAPBBG010000000
VERSION     DAPBBG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.117"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..1150
                     /locus_tag="RD477_002753"
     CDS             5..1150
                     /locus_tag="RD477_002753"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDT7635053.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLATSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="RD477_002754"
     CDS             1321..1779
                     /locus_tag="RD477_002754"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDT7635054.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="RD477_002755"
     CDS             1938..2408
                     /locus_tag="RD477_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDT7635055.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            2541..3968
                     /locus_tag="RD477_002756"
     CDS             2541..3968
                     /locus_tag="RD477_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HDT7635056.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(4002..4117)
                     /gene="rrf"
                     /locus_tag="RD477_002757"
     rRNA            complement(4002..4117)
                     /gene="rrf"
                     /locus_tag="RD477_002757"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1382 a          770 c          771 g         1252 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagcgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct cagactgtag
     2461 acaaactcct aaattgggag tgttgtttgc agtttttttg ttttatactc attgtataaa
     2521 ctattctggg agtgattcta atgatgagaa aacaatcgat tgaaggtaga aatcaatttg
     2581 ctatgttaac aattgatgat cttgtaccta aagatcactt agttcgaaag attgatgctg
     2641 cgattcagtt tgatttcatt tatcccattg tagaatctac gtattcgacc catggtcgtc
     2701 ccagtattga tccagtggtc ttaattaaat tagtatttat ccaatattta tttggtatcc
     2761 gttcgatgag acagactatt aaagacgttg aaacaaatgt tgcttatcgt tggtttttag
     2821 gttattcatt cgaggatcct atcccgcatt tctctacttt tggtaaaaac tatgttcgaa
     2881 gattcagaga aacaacttta tttgaagaca ttttctctca tattctagaa caagctgtaa
     2941 aagctggctt cgttacggaa gacaaccttt atattgattc tactcatatt aaggcaaatg
     3001 ccaataaaca taagttcaca aaagaaatga cctacggtca agctaaagct tatcaagatg
     3061 aattagaaga tgaaataaat aaagaacgta ttgctgtagg aaaacgtcct tttacatggg
     3121 acacagaaag tgagttgagt cttcaaaaaa taagtcattc agatccagaa agtggttact
     3181 atgttaaagg tgaacgagaa aaacaatttg cttattccgc gcatacttcc tgtgaagata
     3241 atggttttat cttatctaca ttaatcacac caggaaatgt acatgacagc caagtcgcaa
     3301 tcgatctggt caaacagtcg aaaagagctt ttccaaatat aaagcgtgtt gtcgctgatg
     3361 caggatataa gacgcctaaa tttgttcatt ttctgtcaca cttgaaactc tacccgattc
     3421 ttccatatac tgtacctcat ggagtaaaag gaatgtttcg taaaaaagac tttgtttatg
     3481 atgcctattt tgactgctat ttatgtccca ataatcaacc gttgttattt tcaactatca
     3541 ctcgtgatgg ttatcgaacc tataaatcaa atccaaaatt ctgtaggagt tgtccattac
     3601 ttaaaaactg tactttgagt acaaaacatc aaaaaatagt aactcgacat gtttgggcgg
     3661 atcttatgga tgaacttgaa caccaacgcc atacggcgtt gaatagagaa atttataaaa
     3721 agagaaaaca aaccattgaa cgaatatttg cagatgcaaa agaaaagcat ggtatgcgtt
     3781 ggacgaaata tcgaggtctc gaaaaagtcg ccacgcacac gatgcttacg tttgctgcca
     3841 tgaatttaaa aaaactagcc acatggctat ggaaagcaaa aaagcctatg gctttttatt
     3901 taaaatttct acaaagacta acaaaaagga gttggcaatt aagccactcc tttttgtcta
     3961 cagtctgaga tgaactattt ctagttcatc tttttgcctt tgcgtggcga cgtcctactc
     4021 tcacaagggg caacccctca ctacaatcgg cgttaagaag cttaacttct gtgttcggca
     4081 tggttacagg tgtatccttc tcgctatcgc caccacactg tggtgttatc ttttattgag
     4141 taaattttgt tcactcaaaa ctggatttga agtca
//
LOCUS       DAPBBG010000112         4079 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPBBG010000112 DAPBBG010000000
VERSION     DAPBBG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.118"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..462
                     /locus_tag="RD477_002758"
     CDS             <1..462
                     /locus_tag="RD477_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7635057.1"
                     /translation="KDDYDFILVDVPPTLSTYTDSALLASDYTIIVLQTQERSLVGAE
                     AYVGYLQELIDNYNADFDILGVLPVLLKNNSKVDEATLNTAKEKFGEENLFENVVKNM
                     ERLKRYDIIGIVDPDLDTKYDIHDKRVMGLYKRVTEEMLKRLEEIESRNKQ"
     gene            504..866
                     /locus_tag="RD477_002759"
     CDS             504..866
                     /locus_tag="RD477_002759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635058.1"
                     /translation="MADNLLNKNKGKRNLLERKEEVKPQQSFNRSSLFTVNESQQTNG
                     DQKKDVPRKKERGTTTTIRCATDTSHRLNAIVTSFNLDSVNELIEILLDNYEASLTTD
                     ERREIKTLLEVYQRKSKK"
     gene            1175..1690
                     /locus_tag="RD477_002760"
     CDS             1175..1690
                     /locus_tag="RD477_002760"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HDT7635059.1"
                     /translation="MVPLTSEKIFGLAPIIDDQAKILILGSMPGKESLKKEQYYANKR
                     NQMWKIMENTLKLQLPEEYPKRINVLRKHHIALWDVIHSCKRVGSLDSAITEDVPNDF
                     GSFFKEYSSLKIVGFNGNKAYQTFKSKIGFQDFPEIQFIKLPSTSPIPGKNVKSFEEK
                     VVEWSKVVNSL"
     gene            1755..2270
                     /locus_tag="RD477_002761"
     CDS             1755..2270
                     /locus_tag="RD477_002761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635060.1"
                     /translation="MIHVEQGLTDIQVVPTALVDSEWKFIKDLTIYLEENERIFEGKE
                     LYLIRNFSRKGIGFFEESGFYPDFIMWYFNGSQEYIIFIEPHGMLRENLDGNKVQLYK
                     EIKKVENEISLSEGTRPVLESFILSPTPFATLNNPINNPSNYSKGDLNRQHVIFMEDE
                     NYIEQLFESLI"
     gene            complement(2370..2633)
                     /locus_tag="RD477_002762"
     CDS             complement(2370..2633)
                     /locus_tag="RD477_002762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635061.1"
                     /translation="MILQVYNEQQGLTGGSSESSSSPESSEPESAAPSTPAGGDVSSV
                     DTNGNGNVTIKEAKAAGYSMPITSDHWLYQYMNDRDNDGMVGE"
     gene            2708..2857
                     /locus_tag="RD477_002763"
     CDS             2708..2857
                     /locus_tag="RD477_002763"
                     /inference="COORDINATES: protein motif:HMM:NF025625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDT7635062.1"
                     /translation="MIEKNGVLRTILDISYNSPSRVAELISGNSTNGWSFVEGLSELR
                     TMDQE"
     gene            complement(2892..3122)
                     /locus_tag="RD477_002764"
     CDS             complement(2892..3122)
                     /locus_tag="RD477_002764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635063.1"
                     /translation="MLKNLFFKNKKQLTILEKIFYFFVGNKNRMQNKSYYVRERAGAK
                     WRYRKFKDPWIAKNRNTLFKRVFGFFEDIFRG"
     gene            3498..4061
                     /locus_tag="RD477_002765"
     CDS             3498..4061
                     /locus_tag="RD477_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7635064.1"
                     /translation="MSGYIYGYARVSTRSQELNRQIDLLNEQNCNEILTEKMTGTKAD
                     RPQLNRLKDKVRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHEVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQRDIERAVKLYDSQ
                     IYSIKEITEMTGISKATLYRYLKTNNF"
BASE COUNT         1449 a          668 c          740 g         1222 t
ORIGIN      
        1 aaggatgact acgattttat tttggtcgat gttccaccta ctttaagtac ttatacggac
       61 tcggctttgt tagcttccga ctacaccatt attgtcctac agacgcaaga acgttctttg
      121 gtaggggccg aagcttatgt agggtacttg caagaactca ttgataacta taatgcggat
      181 tttgatattt taggtgtact acccgtatta ctaaaaaaca attcaaaggt cgacgaagct
      241 acgctcaata ctgcgaaaga aaaatttggt gaagaaaatc tcttcgaaaa tgtagttaaa
      301 aatatggaac gattaaaacg atatgacatt attgggatag ttgatcctga tttagacact
      361 aaatatgata ttcacgataa gagagtaatg gggctttata agcgtgtcac tgaagagatg
      421 ttgaaacgat tagaagagat agaatctcga aacaaacaat agacattatt cataccatat
      481 acaagaaaga agagggtgat agtatggcag ataatttatt aaataaaaat aaagggaaac
      541 ggaatcttct tgagcgaaaa gaagaagtga aaccccaaca atcttttaat cgatccagtt
      601 tatttacagt aaacgaatct cagcaaacta atggagacca aaaaaaagac gtccctcgaa
      661 aaaaagaaag gggcacgaca actactattc gttgtgcaac ggatacttca catcgtctga
      721 acgcaatcgt tacttctttc aatttagata gtgttaatga actgatagaa atcctcctag
      781 ataattatga ggcttcttta acgacggatg aacgaagaga aataaagact ttactagagg
      841 tttaccagcg aaaatctaaa aagtagattt gtagatttgt agacacgtct acatgtagac
      901 gtgtctcttt aatcgtgtaa atttcgctat ataatggcaa ttacaatgat gtagtcaaaa
      961 aaatgggaag gacttttgta aacttatacg ttgaaaattt aaacaatagt gacaccaaac
     1021 gttttgagga tttagttcaa atatacgaag ataaagtcgt caatagacac caaaaaaagc
     1081 taaaaaaaac attctagtat actacaagga ttagatacca taacgtttta aaaaattaac
     1141 tcttatgtga ctgaactaca aaggggataa aaatatggtt ccattaacct cagaaaaaat
     1201 atttggttta gctcctatta tcgacgatca agctaaaatt ttgattttag gatccatgcc
     1261 tggaaaagaa tctctcaaaa aagagcaata ttatgctaac aaaaggaatc aaatgtggaa
     1321 aattatggag aacaccctca aattacaact ccctgaagaa tatccaaaac gaattaatgt
     1381 tctaagaaaa caccacattg ctttatggga cgtgattcat agttgtaaga gagtagggag
     1441 tttagattct gccatcacag aagatgtgcc aaatgacttt gggtcttttt ttaaggaata
     1501 ctcatcctta aaaattgttg ggttcaatgg gaacaaagca tatcaaacat ttaaatcaaa
     1561 aatcggtttc caggattttc cagagattca gtttattaag ttaccttcta ctagcccaat
     1621 tccagggaaa aacgtaaaat cgtttgaaga aaaagttgta gagtggagca aggtagtaaa
     1681 tagcttataa acatttaggt attgatttga aaatcagagc ctactaaaag aaacataaat
     1741 ctttatataa cccgttgata catgtagaac aaggactaac cgatattcaa gtagtcccta
     1801 ccgcgttagt tgatagtgaa tggaaattta ttaaagactt gacaatctac ttagaggaaa
     1861 atgaacgaat ttttgagggt aaagaacttt acttaattcg taacttctca agaaagggaa
     1921 ttggattttt tgaagaatct gggttttatc ccgattttat catgtggtat ttcaatggca
     1981 gtcaggaata tataatattt attgagcctc acggtatgtt gcgagagaat ttagatggta
     2041 ataaggtcca gttatacaaa gaaattaaaa aggtagaaaa tgaaatttca ttgtcagaag
     2101 gtacccgtcc agttctagag tcctttattc tatctcccac accatttgct acattaaata
     2161 acccaattaa taatccatct aattattcta aaggtgactt aaacagacaa catgtcatat
     2221 ttatggaaga cgaaaattat attgagcagt tatttgaatc actaatataa aaacagataa
     2281 aagagactaa atttccgaat gaactgcccc ctgtctactt gacagggggc agttcattaa
     2341 tctggtcttt catttttcat tattttggtt tattctccaa ccataccgtc attatctcta
     2401 tcattcatat attgatataa ccaatgatca ctagtaatag gcatagaata gccagctgct
     2461 tttgcttctt taatggttac attcccattt ccattggtat ctacagagga aacatctcct
     2521 ccagcaggag tacttggtgc ggcagattct ggctctgagg attcaggaga agatgaagat
     2581 tcactacttc ctcctgttaa accttgctgt tcattgtaga cctgtagaat caagtgcgca
     2641 atggaagact ggtaatttct ttattcgcta taacgatgtg attgacagtt acattgaaga
     2701 tggaaaagtg atcgaaaaaa atggagtttt gagaacaata cttgatattt cctataatag
     2761 tccttcgaga gttgctgaat taatatcagg aaactcaaca aatggatggt catttgttga
     2821 aggtttaagt gaattgcgga ctatggatca ggaataaaaa agaggcatgg gcctcttttt
     2881 tatttggaaa tttatcctct aaatatatcc tcaaaaaaac caaagactct tttgaatagg
     2941 gtgtttctat tttttgctat ccatggatct ttaaattttc gatatcgcca ctttgcccct
     3001 gcacgttctc gaacataata ggacttattt tgcatgcggt ttttatttcc tacaaaaaaa
     3061 tagaatattt tttctaaaat tgttagctgt ttcttattct taaaaaataa gtttttaagc
     3121 attcctttcc cccttggctc agccttaata ctgtgtaagt atctaactac tatattagca
     3181 tacccctaaa ctaaaaatac gttaaagtgt gcctaagtaa aaattatatg gcacaacagg
     3241 ttaaacaaat tttggtttat tgtttccaga aaataatcaa ctccttaaac ttaaagcttt
     3301 tagataataa ctcaaaaaat taagtcagtt aaaattaaac ctgaagtctc aaaaacaatc
     3361 gattatgaga cgattttaaa ggacggattt ttcacctaaa aagtagtcct ttttagtctc
     3421 aaaacttctc tctttatgta tgcaccttta actcccgttt atgatacaat aatatcaaaa
     3481 acaaaggatg attttttatg agtggatata tatatggcta tgccagagtg agtacacgct
     3541 cgcaagagtt gaatcgacaa atagacctac tcaatgaaca aaattgcaat gagatcctaa
     3601 cggaaaaaat gaccggaacc aaagccgatc gtcctcagtt gaatcgacta aaagataagg
     3661 tgcgtcctgg agacacggtt atcgtggaaa gtttctctcg tctaggtcgg agtaccaaag
     3721 atttaattga tctcgttacc tactttgaag aacatgaagt gaagctggtg agtttgaaag
     3781 aaaacttcga tactagcact cctcacggac gcttaatgat gaccgtcttc caagcgttta
     3841 gtcagtttga acgtgatttg attgtggagc gtaccaaaga agggttaaaa agtgcgcgtg
     3901 ctagaggccg aaaaggagga cgcccacgcg ttaatcaacg agatattgag agagcggtga
     3961 aactgtatga cagtcaaata tacagcataa aagaaattac agaaatgact ggcatcagta
     4021 aagctacact ctatcgctat ttaaaaacaa ataattttta aatcttatca tgaaatgaa
//
LOCUS       DAPBBG010000113         4051 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPBBG010000113 DAPBBG010000000
VERSION     DAPBBG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.119"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1023..2036
                     /locus_tag="RD477_002766"
     CDS             1023..2036
                     /locus_tag="RD477_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7635065.1"
                     /translation="MTKKNRVTIRDVAQAAGVSISTVSKALNSLDGVSPETRVEIQNI
                     ASRLNYVPNLMGKQLKSGKTKMIGVYTNRISGPYFNSLIDEIAFESKLHGYGLNVIVS
                     DERDVIMSSLLGNFVDGAIVVEDVLNQKDIETINSNGVATVFINRIVADKKVGSVAFD
                     SFEKGYLAGNYLLNLGHKKIGYVNGVDDIYDNDQRFQGFKAALSKVGLTYNEDFTIAG
                     NFVEEDAYANTLAFIDNQPAELPSAFLAANDMSAVGLIKALTEKNYSVPEDFSVVGFD
                     DVEVLKYFKPSLTTIHNSTKEQGKKAIEHLLEMMGGKSVGRVIEVKGTLMIRETAIEY
                     TGE"
     gene            2043..2393
                     /locus_tag="RD477_002767"
     CDS             2043..2393
                     /locus_tag="RD477_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7635066.1"
                     /translation="MYVGIDVGGTSIKYGLVDSKGNIVAKDTLVTSFEKEELLQNLAD
                     IVEKYVSSSPETIEGVGISMPGIVQEDGFLLTAGAIRSCYGINLKEEMEKRTGILTKE
                     VIDSNGANLDWENK"
     gene            2496..3860
                     /locus_tag="RD477_002768"
     CDS             2496..3860
                     /locus_tag="RD477_002768"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HDT7635067.1"
                     /translation="MNKKSAILLLSCGVFLSLATERSFATEENKTNHVSAETALVATS
                     KTTVSKDNPTVFDFPFNGEDNRMHRADGWSNGNMFNTTWRASQVNFEDGKMQLSINKD
                     EENTKIPYISGEYRSNQNFHYGLYEVNMKPIKNDGVVSSFFTYTGPSENNPWDEIDIE
                     FLGKDTTGVQFNYFTNGLGNKEYYHNLGFDASEAFHTYAFEWLPDSITWFVDGEKIYT
                     ATENIPQTPGKIMMNVWPGTGVDSWLNPFDGTVPLKAEYDWIKFTQYKIVEEDVTPKP
                     EEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTTEPTTE
                     PTTEPTTEPTTEPTTEPTTEPTESSTTGSSTTESSTTNSSGNTTPPATTETTNTTSSS
                     TSQSSKAAGKTTTPKNNSTTSKKDLPATGENTHASYWTAFGGMLILAGLGGALFFKNK
                     NRKA"
     gene            3874..>4051
                     /locus_tag="RD477_002769"
     CDS             3874..>4051
                     /locus_tag="RD477_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635068.1"
                     /translation="MKAKGVMLVLGCSLLLGLVINRSLAHEKKSKNSGEETESTMVEN
                     KNNAKAQTTVFDFPL"
BASE COUNT         1425 a          678 c          834 g         1114 t
ORIGIN      
        1 acaaaaagaa agatcttttg cccattttat ttttataaaa tgggtaggtg gcgttcgcgt
       61 aaagcaaatc gatacaatcc aatggttttg gcgaatcaga tttaccattc taggcttgtt
      121 taatacagac atcgattgtt attttgaaaa tttatatcct tattggcaat gaaatggata
      181 ttctccatat caatagaaat cttcaagata attttcggaa atatataaga gttaatcaat
      241 tatgatcata tagtaggtaa aaattaggac agccttcaat tatatgggaa accaatataa
      301 tcaagtaatt attcgggtaa cacaaatact agagtactca tttcttataa ctgttttaca
      361 cctaaaaatg agcttatcgg aaactttgca acagaaccag ttagctgtta catttaagat
      421 aatttcaaaa tcttgatcta ttaattagaa agtagggggt aaggtgggga aaaagaattt
      481 tgttttattt ttatttgaaa cgatggtcca gctttggtcg ttatttttat ttctgttaat
      541 tatttataaa tacttttttg gaactagttt ttcttttatt acgatcactc ggccagaatt
      601 ttatttaacg ggtttagtta gtcttgtttc tacgatcgtt ttatattttg tccgatcgcg
      661 aaagtaaaca aataaattct taaagccaac tccctttttg taaggagttg gcttcttact
      721 ttcctccgcc cacaagttca tttacctaac gtgtgaaaaa cattcccata atttctaatt
      781 aaataattta tttttattat aaaaacgttt tccttttctg gtaaaagagc aggcgttaga
      841 aatccccaag ctaaaaggtt tttccctgat tcaaacacga aaaacaaatg tatacgttaa
      901 caaaggcgtt aaattagctt ttatttttta taaaaaaagc ttgtatccaa aaaaatacag
      961 tgttataatt caatcgaagt aaagaaaaac gttttcttta aaaagtacat gaagtgaata
     1021 aaatgacaaa aaaaaatcgg gtaacgatta gggatgtggc acaagcagct ggcgtttcaa
     1081 tctcgacggt ttccaaagcg ttaaacagtt tggatggcgt ttcacctgaa acccgggtcg
     1141 aaattcaaaa tattgccagc cgtttaaact atgtgcccaa cttaatgggg aaacagttaa
     1201 agtctggtaa aacgaagatg atcggggttt atacgaatcg gatcagcggt ccttatttta
     1261 actcactaat tgatgagatt gcttttgagt ctaagttaca tggatatggt ttaaatgtaa
     1321 ttgtctctga tgagcgggat gtaattatga gtagtctgtt aggtaacttt gttgatgggg
     1381 cgattgtggt ggaggatgtc ttaaatcaga aagacattga aaccattaat tccaacggcg
     1441 tggcgacggt ttttattaac cgcatcgtag cggataaaaa agtcggcagt gtggcctttg
     1501 attcgtttga aaaaggttat ctggcgggga attatttatt aaacttaggt cacaaaaaaa
     1561 ttggttatgt caacggggtc gatgatattt acgataacga ccaacgtttt caaggattta
     1621 aagcagcctt atcaaaagtc ggtttgactt ataatgagga ctttacgatt gccggtaact
     1681 ttgtagaaga agatgcctat gccaatacgt tagcctttat tgacaatcaa ccagcagagt
     1741 taccgtcggc ctttttagcg gcgaatgata tgagtgcggt ggggttgatt aaagccttaa
     1801 ctgaaaaaaa ttattctgta ccggaagatt ttagtgttgt tggttttgat gatgtggaag
     1861 ttttgaaata tttcaaacca agcttaacaa cgattcataa ttcaacgaag gaacaaggta
     1921 aaaaagcgat tgaacattta ttagagatga tgggtgggaa atcagtcggt cgtgtgattg
     1981 aagttaaggg cacgctgatg attcgagaaa cagcaataga atacacagga gagtgattga
     2041 gtatgtacgt tgggattgac gttgggggca caagtattaa atacggttta gttgattcaa
     2101 aaggaaacat cgtggccaaa gatacgttag tcacgagttt cgaaaaagag gagctcttac
     2161 aaaacttagc ggacatcgtg gagaaatatg taagcagttc gcctgaaaca atcgaaggtg
     2221 tgggaatcag catgccaggg attgtccaag aagatgggtt tttattaacc gctggggcga
     2281 ttcgttcttg ttacggtatc aatctaaaag aagagatgga aaaacgtacg gggattttaa
     2341 caaaagaagt gattgattca aacggggcta acttagactg ggaaaataaa taagtcacaa
     2401 agaaacgtca aacattaaca gaaaaataaa tgtttggcat ttcctttacg aaaaaatgaa
     2461 aacgattgct taaaaacaat aactggaggg aatttatgaa taaaaagagt gcaattttac
     2521 tattaagctg tggggtattt ttaagtttgg ctactgagcg gagcttcgca acggaggaga
     2581 acaaaacaaa tcatgtgagt gctgaaacgg cactagtcgc caccagtaaa acgacggttt
     2641 ctaaagacaa tccaacggtc tttgactttc ctttcaatgg agaagataac cggatgcatc
     2701 gggccgatgg ttggagcaat gggaatatgt ttaatacaac gtggcgggcc tctcaagtaa
     2761 acttcgaaga tggtaaaatg caactttcga ttaataaaga cgaggaaaat acaaaaattc
     2821 cgtatattag tggggaatac cgttccaatc aaaacttcca ttacggttta tacgaagtta
     2881 atatgaaacc aattaaaaat gatggggtag tgtcatcttt ctttacgtat accggcccaa
     2941 gtgaaaataa tccctgggat gaaattgata ttgagttttt aggtaaagat acgactggcg
     3001 tacaatttaa ttattttacg aatggcttgg gcaataaaga atactatcat aatttaggtt
     3061 tcgacgcctc agaagcattc catacgtatg cctttgaatg gttaccagat tcgatcacat
     3121 ggtttgtcga tggtgaaaaa atctataccg cgactgaaaa tattccgcaa acaccaggca
     3181 aaattatgat gaatgtttgg ccgggtactg gagttgactc atggttaaat ccctttgatg
     3241 gaacggtacc attaaaagcc gaatacgact ggattaaatt tacgcaatat aaaatcgttg
     3301 aggaagatgt cacgcctaaa ccagaagaac caacgacaga accaacgaca gaaccaacaa
     3361 cagaaccaac aacagaacca acaacagaac caacgacaga accaacaaca gaaccaacga
     3421 cagaaccaac aacagaacca acgacagaac caacaacaga accaacaaca gaaccaacaa
     3481 cagaaccaac aacagaacca acgacagaac caacgacaga accaacgaca gaaccaacaa
     3541 cagaaccaac aacagaacca acggaatctt caacaacggg aagttcgaca acggaatctt
     3601 caacaactaa cagtagcgga aatacgacac caccagcaac aactgaaaca actaacacaa
     3661 cctcaagctc aactagtcag tcgtccaaag ccgcaggcaa aacaacgacg ccaaaaaata
     3721 atagtacaac tagtaaaaaa gatttacctg ctacaggaga gaatacacat gctagctatt
     3781 ggactgcttt tggcggaatg cttattttag ctggtctagg cggcgcctta ttctttaaaa
     3841 ataaaaaccg taaagcgtaa tcggagggga tttatgaaag cgaaaggtgt tatgctagta
     3901 ttaggctgta gcttattgct tggtttagta attaatcgca gtttggccca tgagaaaaaa
     3961 agtaaaaata gtggcgagga gacggagagt acgatggtag aaaataaaaa taacgctaaa
     4021 gctcagacaa ctgtctttga ctttccacta g
//
LOCUS       DAPBBG010000114         3988 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPBBG010000114 DAPBBG010000000
VERSION     DAPBBG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.120"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            131..505
                     /locus_tag="RD477_002770"
     CDS             131..505
                     /locus_tag="RD477_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7635069.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="RD477_002771"
     CDS             complement(619..1248)
                     /locus_tag="RD477_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7635070.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="RD477_002772"
     tRNA            complement(1382..1454)
                     /locus_tag="RD477_002772"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="RD477_002773"
     tRNA            1668..1741
                     /locus_tag="RD477_002773"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="RD477_002774"
     CDS             complement(1879..2043)
                     /locus_tag="RD477_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635071.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="RD477_002775"
     CDS             2338..3666
                     /locus_tag="RD477_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDT7635072.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..>3988
                     /locus_tag="RD477_002776"
     CDS             3868..>3988
                     /locus_tag="RD477_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HDT7635073.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLK"
BASE COUNT         1357 a          615 c          747 g         1269 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaa
//
LOCUS       DAPBBG010000115         3970 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPBBG010000115 DAPBBG010000000
VERSION     DAPBBG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.121"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..356
                     /locus_tag="RD477_002777"
     CDS             <1..356
                     /locus_tag="RD477_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7635074.1"
                     /translation="EIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKS
                     LEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGN
                     VFKIEYTKKRQRLAY"
     gene            591..827
                     /locus_tag="RD477_002778"
     CDS             591..827
                     /locus_tag="RD477_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635075.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            834..2786
                     /locus_tag="RD477_002779"
     CDS             834..2786
                     /locus_tag="RD477_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDT7635076.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="RD477_002780"
     CDS             complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="RD477_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDT7635077.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3585..>3970
                     /locus_tag="RD477_002781"
     CDS             3585..>3970
                     /locus_tag="RD477_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7635078.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1415 a          640 c          697 g         1218 t
ORIGIN      
        1 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
       61 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga
      181 attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc
      241 ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
      301 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tattaagtag
      361 ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa tgagtatcaa attgcaactg
      421 aatttgatac tcgtttttta ttgttaaatc aacgtttgtt gttaatttac accacaaaat
      481 gtaagttaaa atgtatataa aaatgcgaac gtgaaataat tattgtagga atgcaaattg
      541 gtgtgttaaa atggttacaa tttactaagc aaaaaggggt gcattttcct atgcaatttc
      601 ttgagccaaa aaataagaac gctaagtctg tggattggga aatatcagag caagtaaggg
      661 tgatagttaa gcaatacgct gaatatgcag agcgtactga aagcgaagca gtggatgaat
      721 tcttattgaa cattttagac gataagaagt ttattgaatg gattgccaac aagcgaagta
      781 ataagcggat tgttgaaaaa atgggtatca aagatagagt gggttgatag acaatgacca
      841 agcttaaaag attagccacg aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa
      901 acgaagaatt aaaagatagg gctaaacaat atgatttatt aactccaaaa cgatttgcaa
      961 caaggtataa caaaatgttg ttcttgccaa cgtcttttaa atggaacggt tctgaatatc
     1021 ccattcaata taattactgc atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca
     1081 aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat gactggtagt tccaaagata
     1141 aaggtcataa aggtatgtac tgcaatgaca atcctattgg cagaggtgta tcgcaaaact
     1201 gtacagtaac ccctttatct aattggtctg ttgttgaaga aattaaacgc cttatagaaa
     1261 tcaatagcat ccaagatgtt gagccagatt atcaatttca taaagaaggc tgctcggaag
     1321 aagaatcaac cccattcaat gagccgaagc aattttacaa aagggggaaa agtaggggca
     1381 aatctcaacg ctatcaatgt aaggcgtgta agaagttcac taatgttcta ccaaaaagag
     1441 aagaaaccac tacttatcac caacaaaaga acaccattct tccaatgttc gctaagatgg
     1501 ttgttggaag ggtttcagtc agtcgtactt gtgatatttt aggaattgga gttggaacct
     1561 actatcataa gctggagtgg ctgtataggc gttgtttgga gtttttagaa cgatatgaga
     1621 cacaacccct tcaaacaaag aaatttaacg agatgtggct taatacggac aagatgcact
     1681 actacctaaa taacgtccgt aagaaaggac aaggttcaaa gaaatatacg gggtttgaag
     1741 accttaatat gcaaacgtat atagttgtat ctgctgaggt cttatcaaga tatgtgttcc
     1801 gttctgatgt tgcctatgat tggaatattt caatggatga attaaatgaa gatacaagaa
     1861 aattcaaaga agaccatctc aatactttta gtcgtaaaaa tgataggtta gattggtctt
     1921 attatccaca agaaccatcc gctaacgata gcgaaaatag gaatgcttat cttcacgaat
     1981 tagggaaaat tactaatagg agtagatttg tcgatggttt aaatgttgat gctccttata
     2041 ctacaactgc tcactattgg ttaattaaac aaatggttaa tgccgacgaa tggcgaatga
     2101 ttagtgatga tgacttctct attagaaacg ccttttatag agtattcacg aaagaattaa
     2161 ggttgagcga tgcacatcat tttatttgcc aagttaacaa gacgaaaagc agaaaacaat
     2221 gcttaaaaga attcggacaa gcaaaagcag aactgctcga ttggggagat atcagaggat
     2281 tcaaaacaaa gtttctacgg actttggcat ctcattatct aactgaatta ctaacaagtc
     2341 atcaattcca cgaagaggct attagtaaag atggagaaag atatcgcaaa tatgccgata
     2401 accctatcaa acatccatta gccactaagg ataaaggatt ttattcagtt gattgtagaa
     2461 cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc
     2521 attcgaccaa tagctttatt caacagataa gacgttatat atcatctctt gaaagaccat
     2581 taacaacagc tcgtggtgat aagaagagct atatttacgc taacttcaat ccgaagtatg
     2641 ctcaatttgc gattacaatt ctaagaactt actacaactt ttgtcgccct tttaaatcag
     2701 cggataaaaa ggttttgaca cctgctcaaa gattagggat tacagataaa caatttgatt
     2761 ggaaagatat aatttatttc aaatgacaaa aagagcttgg agtaatccca agctcttgtg
     2821 ctttatcttt actgttcttc ttcaattaac gcacccgtta actcaataag ttttttcttt
     2881 catatacatg aaaatagtag ttatacggat ttttatcatt ctttatccct ttttgggcaa
     2941 atacctcatt ccattcctca taattcactt ctggaaaaaa agtatctcct tcgaattcat
     3001 gatgtatttt tgtgatgtac attttctcaa cataagggaa aaacaaatta taaatctgtt
     3061 ctcctccgaa aataaaaatt tcttcttcgt ttttacataa ctcaaaaaca tcttctattg
     3121 aatgaacaat ttcacaacca ttaaaggtaa accccttatc tctcgtcaga ataatatttc
     3181 ttctgtcagg taaggctctt ccgattgatt caaggttctt cctacctaat attatcggat
     3241 gtccctttgt agtattttta acatattccc agtccttggg aatcctccaa ggaatgtcat
     3301 tctctttgcc aatcactcta ttcttatcca tcgcagcaat caaagaaact ttcatttata
     3361 acacagcctt tttaactaat ttaccatcta atttcttatt tctatcaaaa aaccttttct
     3421 cattatcctg cccgttagtt gaataagcaa ttttttctat aagttatatt atcataatac
     3481 cacaaaatca caaattttca ttcaattatc attttcaatt gcattttata aatcattttg
     3541 tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc cattttgaag tacattttgt
     3601 cattttgtac tccaaaactg aactacttac ttgcatatat acgcctttat agtggagtac
     3661 tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt
     3721 atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta
     3781 ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac
     3841 agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac
     3901 ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc
     3961 tacgatatta
//
LOCUS       DAPBBG010000116         3956 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPBBG010000116 DAPBBG010000000
VERSION     DAPBBG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.122"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..156
                     /locus_tag="RD477_002782"
     CDS             <1..156
                     /locus_tag="RD477_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDT7635079.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="RD477_002783"
     CDS             175..1452
                     /locus_tag="RD477_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7635080.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="RD477_002784"
     CDS             1462..2118
                     /locus_tag="RD477_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7635081.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="RD477_002785"
     CDS             2118..3494
                     /locus_tag="RD477_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7635082.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="RD477_002786"
     CDS             3624..>3956
                     /locus_tag="RD477_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7635083.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAPBBG010000117         3912 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPBBG010000117 DAPBBG010000000
VERSION     DAPBBG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.123"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(256..1629)
                     /locus_tag="RD477_002787"
                     /pseudo
     CDS             complement(256..1629)
                     /locus_tag="RD477_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343224.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1747..3333)
                     /locus_tag="RD477_002788"
     CDS             complement(1747..3333)
                     /locus_tag="RD477_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDT7635084.1"
                     /translation="MAFTFQINNDVVWDYDQKLLKKAEENIPKLIETEIQPIRIVEKI
                     PAQQRTNEFKLQDSQKDICTLAQYSLGRGESIILDLGDHYVGQFSIDIDSVGSPMDAP
                     LYLRLKFAEVPSELYARSEDYEGWLSRSWIQEEYLHLDTLPTTLQLPRRYSCRYVELT
                     VIDTSPKWQASFSSPKFIAKSSVSMAQVPTMTIEDNTLKAIYDVSVKTLLDCMQTVFE
                     DGPKRDRRLWLGDLRLQALANYYTFNDLSLVKRCLYLFGGMRTNEGKIPANVFTEPKL
                     TPDDTFLFDYSLFFVTSLYDYVAHTKDKNVLADLYLVAKKAIDLALKQVDIEGRLVLT
                     EDFPVFVDWSNDFDKATAGQAIMIYTLKRFLKLAKLSNEANEMYYQEILEKMINFSNQ
                     TLFNHEKKQFVIEKNNEINVASQVWMVLAEVFDKQTNKEIMLETIKYHFPIQGIATPY
                     MYHHVVEALFIADCQEEAIKLMKEYWGKMIELGADTFWEAFKPEDLTFSPYGSPILNS
                     YCHAWSCTPAYLIGKYLIGK"
     gene            complement(3361..>3912)
                     /locus_tag="RD477_002789"
     CDS             complement(3361..>3912)
                     /locus_tag="RD477_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7635085.1"
                     /translation="QFQTYALTNAGASQTQATGLGIILNVVALGTTIVFTSISGGKYR
                     NKAFFVGAFVQFAAMLGMVLIGGGAGFIALATTIAFYNFGNPLAGEAIYKVWTQESFP
                     AETRASLQGFINGFSRLCCGLFAFVTPYLVMPDHIQSSMIGFAWIVLVATIAGIVMMR
                     LQKKYGTSETRGTEDLQDTNVTA"
BASE COUNT         1318 a          769 c          607 g         1218 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tacgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaaagag gtcgtgacta aaaacaagtc acaacctctt gttacttagt attctcatct
      241 aagttacttt tcttatcatt tgacatccaa agatttcttc atttccaaat cggcctttat
      301 ttggggataa atattatcta gatgataaaa catcattgtt actatcgaac agataatcaa
      361 gagcattggt acataaagat acatcgcttg aatggcaaaa attgcctttc cggattgtct
      421 gatttctcca ggttgataca ttccccaagc aagtaaggca cccgctacac caccagaaat
      481 tgcctgtgca actttcccta aaaagccatt aattgcagaa agtgaaccag aaacattcac
      541 acctgtcttc caaacaccaa aatcaactgg atctgcttgc atcgaaaaat aaatactttc
      601 ttttactccg taaccaatgg ctgaaataaa ggtaccaaca agtatcagtg ttatattctt
      661 agcagcaaac atgataacta tcaatcctaa taactggata cttgcggata aaagatataa
      721 atttcttttg cctaatagtt tagcaagtat aggaacaaaa aagtttgcaa acataggaac
      781 aattgacata atcgtcgcta cagtaataga aagtgtcttg cttccaatca ctacagaaaa
      841 ataataaact agtgcacttg attaaataaa aaaactggtc cacataaaca ggatattgac
      901 tgcaaagatc atccaaggtt tattttgctt caaagacgta aagacccttt tgaatgaagg
      961 attttttgtt ccatggacag gcaattctcg aacagcggag aatgtaaaaa agaatagtag
     1021 acagctgatt acgccaaaca ttcccatgac aatcttgaag cctaattgtt catttccctt
     1081 accaaaaaca gcaacaagtg tcaaaacaga tgaactaaca atcgttgcac ctaggaaagc
     1141 aaaaaatttt cgagaagttg atagtgccgt tctttcttct gtatcatttg tcaaacttgg
     1201 caaaatcgat gccattggta catttactaa tgtatatgca aaagacaaac caagatatgt
     1261 gacatatgca tacactaatt ttcctgaatt agaaaaatca ggcacattga aacacaaaaa
     1321 agcaaaaagt gcaaaaggaa ctgctccaaa aagaaaatac ggtcgtgatt ttccccagcg
     1381 tgtattcgtg tgatcgatca agtagccaac caataaatct gtcacaccat ctacaaaacg
     1441 cgtcactaca aacatcaggc tgattgctgc agcgttcaaa ttcatgatat ccgtataaaa
     1501 atataagaga tagagcgaaa ttacttgcca gataagatta catgcgaagt cagatacccc
     1561 ataaccaatc cgttgtcgcc attttgtcaa ccactttctt tttaatagac tcatttgtgt
     1621 tgtttccata ataagcctcc tttactatat ctgccaacag agataacagc tttcattttt
     1681 tcttaaatca cggatcaatt cattaacaag taagaactga tccatgtatt tttcctaatt
     1741 aactaactat ttacctatca gatatttccc tattaaatag gctggtgtac aactccatgc
     1801 atgacaataa ctatttaaaa ttgggctacc atatggagaa aaggttaaat cttctggttt
     1861 aaaagcttcc caaaatgtat ctgcacctaa ttcaatcatc tttccccaat attctttcat
     1921 caattttata gcctcttctt gacaatcggc tataaataaa gcttccacca catgatgata
     1981 catataaggt gtcgcaattc cttgaattgg aaaatgatat ttaatcgttt ctagcatgat
     2041 ttccttattt gtttgcttgt caaatacttc ggctaacacc atccaaactt gactggcaac
     2101 atttatttca ttgttttttt caatgacaaa ctgttttttc tcatgattaa atagagtttg
     2161 gtttgaaaag tttatcattt tttctagtat ttcttgatag tacatttcat tcgcttcatt
     2221 cgatagtttc gcgagtttta aaaatcgttt caatgtatag atcatgattg cttgtccagc
     2281 agttgcttta tcaaaatcat tcgaccaatc gacaaagaca ggaaaatctt cagtcagtac
     2341 caaacgcccc tcaatatcaa cttgttttag tgctaaatca attgcctttt tagcaacaag
     2401 ataaagatct gctaagacat tcttatcttt ggtatgcgca acataatcat ataaggaagt
     2461 aacaaaaaag aggctataat caaacaaaaa cgtatcatct ggtgttaact taggttctgt
     2521 aaaaacatta gcaggtattt tcccttcatt tgtccgcatt ccgccaaaaa gatacaagca
     2581 tcgtttgact aaagacaaat cattaaaagt atagtaatta gctagtgctt gaagacgtag
     2641 atcgcctaac cataatcttc gatctcgttt aggaccatct tcaaagacgg tctgcatgca
     2701 atctaaaagt gtttttacag aaacatcgta gattgccttt aaagtattgt cttcgatcgt
     2761 catcgtaggt acttgtgcca ttgaaacaga actttttgct ataaattttg gactactaaa
     2821 acttgcttgc catttaggag aggtatcgat aactgttagt tctacataac gacagctgta
     2881 gcgcctaggt aattgcagtg tagtaggtaa cgtatcaaga tgaagatatt cttcttgaat
     2941 ccaggaacgt gacaaccagc cttcgtagtc ctctgaacgg gcatataatt cagaaggtac
     3001 ttctgcaaat ttcaagcgta aatacaaagg tgcatccatc ggtgaaccaa cagaatcgat
     3061 atcaatacta aattgaccga cataatgatc ccctaaatcc aagatgatgg actcacctct
     3121 tcccagtgaa tactgagcta atgtgcagat gtccttttga ctatcttgaa gtttaaactc
     3181 attggtacgt tgttgtgcag gaattttttc aacaattcga attggttgaa tttctgtttc
     3241 aattagtttt ggaatatttt cttctgcttt tttcaataat ttctgatcat aatcccaaac
     3301 tacatcgtta tttatttgaa aagtaaatgc catatttatt ctccttactt ggtcaatttt
     3361 tcaagcagtg acattagtgt cttgaagatc ttctgtccct cgtgtctcag atgttccata
     3421 tttcttttgt aaccgcatca tgacaatacc agcaattgtt gctactaaaa cgatccaagc
     3481 aaagccaatc atcgaagatt gaatatgatc tggcattact aagtaaggcg taacaaatgc
     3541 gaaaagtcca caacataatc gtgaaaaacc attgataaat ccttgcaatg atgctcttgt
     3601 ttctgcggga aatgattctt gtgtccatac tttataaatg gcttccccag ctaaaggatt
     3661 accaaaatta taaaaagcaa tcgtcgttgc caaagcaata aaacctgcgc ccccaccgat
     3721 taaaaccatt ccgagcatag ctgcaaattg aacgaaagca cctacaaaaa aagctttatt
     3781 tcgatatttc ccaccagaaa tagaagtaaa aacaatcgtt gtacctaaag caacaacatt
     3841 caaaatgatt cctaatccag ttgcttgtgt ttgactagct ccagcattcg ttaaagcata
     3901 cgtttggaac tg
//
LOCUS       DAPBBG010000118         3877 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPBBG010000118 DAPBBG010000000
VERSION     DAPBBG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.124"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(717..3866)
                     /locus_tag="RD477_002790"
     CDS             complement(717..3866)
                     /locus_tag="RD477_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDT7635086.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAPBBG010000119         3718 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPBBG010000119 DAPBBG010000000
VERSION     DAPBBG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.125"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(118..708)
                     /locus_tag="RD477_002791"
     CDS             complement(118..708)
                     /locus_tag="RD477_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDT7635087.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="RD477_002792"
     CDS             1231..1392
                     /locus_tag="RD477_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7635088.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="RD477_002793"
     CDS             1411..1905
                     /locus_tag="RD477_002793"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7635089.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1898..2059
                     /locus_tag="RD477_002794"
     CDS             1898..2059
                     /locus_tag="RD477_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635090.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2543..3566
                     /locus_tag="RD477_002795"
                     /pseudo
     CDS             2543..3566
                     /locus_tag="RD477_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT         1384 a          522 c          625 g         1187 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     2461 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     2521 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     2581 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     2641 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     2701 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     2761 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     2821 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     2881 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     2941 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     3001 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
     3061 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaatcaag agcaattgat
     3121 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
     3181 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
     3241 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
     3301 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
     3361 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
     3421 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     3481 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     3541 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     3601 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     3661 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaa
//
LOCUS       DAPBBG010000120         3654 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPBBG010000120 DAPBBG010000000
VERSION     DAPBBG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.127"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            115..975
                     /locus_tag="RD477_002796"
     CDS             115..975
                     /locus_tag="RD477_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDT7635091.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            987..1814
                     /gene="panB"
                     /locus_tag="RD477_002797"
     CDS             987..1814
                     /gene="panB"
                     /locus_tag="RD477_002797"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDT7635092.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1829..2677
                     /locus_tag="RD477_002798"
     CDS             1829..2677
                     /locus_tag="RD477_002798"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDT7635093.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2687..3082
                     /locus_tag="RD477_002799"
     CDS             2687..3082
                     /locus_tag="RD477_002799"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDT7635094.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3284..3654
                     /locus_tag="RD477_002800"
                     /pseudo
     CDS             3284..3654
                     /locus_tag="RD477_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1425 a          412 c          721 g         1096 t
ORIGIN      
        1 atattatgat aaattaacta tggatgatat ttgaaaaaaa catactgtat atttgattta
       61 atttttattt ttaagctcat atcgtaagaa tgggacttgt agtctcactt ctttatggag
      121 gtgataatta tgaacgttgg ttttattgga gccggcaagg taggttgttc attcgggaag
      181 tatttccagg aacataaaat acaagtaaca ggattttata gtaaaagcga agactcgtca
      241 ttagcagctt cgaattttac atctagtaaa caatatttaa acttaagaga attagtagat
      301 gaaaatgata ctattttcat tacgactcct gatggtcaaa ttcaggaggt atggcaagaa
      361 attaaaaatt atcaaatcaa aaacaaatta atctgccatt gcagtggtgc aatatcttca
      421 gatatctttt caaatataca ggactatggt gcttatggct attctgtcca tcctatgttt
      481 tcaatatcag ataaatataa ttcatacaaa aaattaaaaa aatcttttat aacaattgaa
      541 ggcgatgaaa aatatgcaaa gtttttatgt gatttcttca aaaagttagg taatagtact
      601 gtaattgtat ctaaggagaa taaatctctt tatcatgccg catcagtagt atctagtaac
      661 cttgtgctag gacttattaa tacaagcgtt acatatctaa ttcaatgtgg atttactgaa
      721 aaaatggcaa ttgaagcttt atatccatta attgaattta atattagaaa tataaaagaa
      781 aaaggaatta tagaaagttt gacagggcca gttgaacgtt gtgatatttc aacaattaaa
      841 ggtcactgtg aagttttatc agatgaagac agagctttat atatgctatt atcaaaaaat
      901 gtattagaga tagctatgtt aaaaaatatt aatagagatt atagtgaatt agaaaagtat
      961 ttaggagaga gttaggagag aaatagatga aaaatacagc agttacattt aaagaatcta
     1021 aattaagaaa tgaaaaatta acaatgctta cagcttatga ctattcaaca gcaaaaataa
     1081 ttgatgaagc tgggataaat ggaatattag taggagattc tcttggtatg gtatgtctag
     1141 gacatgaaga tacactttca gtaacaatgg aagacatgat tcatcatact agagcagtta
     1201 caagaggagc taagaacact ttagttgttg cagatatgcc atttatgtct tatcaaactt
     1261 cagtatatga ttcagtagtt aatgcaggaa gattaataaa agaaggacga gctcaggttg
     1321 ttaaattaga aggagggata gaagtatgcg ataaaataga ggctatagta aaggcttcta
     1381 tacctgttat ggcgcatata ggtttaacac ctcaatctgt taatgcattt ggaggattta
     1441 aggtacaagg aaaggacaag gaagctgcta aagaattaat aagagctgct aaagctgtag
     1501 aaaaagcagg agcattcgct gtagtgttag aatgtgttcc aactaagctt gcagagttaa
     1561 tttctaaaga gatatcaata cctacaattg gtattggagc aggtgcaggg tgtgatggac
     1621 aaattcttgt atatcaagat atgttgggga tgtatagtga ttttacccct aaatttgtga
     1681 agaaatatgc taatttatca gaagagatga ataaagcatt tacaaaatat atagaggaag
     1741 taaaagatgg agtttttcca ggtccagaac atgggtttaa aataagtgat gatgttttag
     1801 aaaaattata ttaaaagggt caagaaatat ggttatattt aaaacagtga atgatgttag
     1861 aagtcaagta aaagagtgga aaaaacaagg cttaaaggta ggattagttc ctactatggg
     1921 atatttacat gaaggtcatg aaagtttaat tagaaaagca tcaaaagaaa atgataaagt
     1981 tgttgtaagt atctttgtaa atccaactca gtttgggaaa aatgaagatt taggtagtta
     2041 tccaagagac ttagaaagag atatagaagt atgcacaaga ggtagagcaa cagcaatatt
     2101 taatcctgaa gttgaagaga tgtattgtga caatgcaagt acttttgtta atataactgg
     2161 attaacagaa ggtttatgtg gagctagtag gccaatacat tttagagggg tttgcactgt
     2221 tgtaagtaaa ttatttaaca taataccagc agatagagct tattttggag aaaaggatgc
     2281 acaacagtta gctgtaatta aaagaatggt tagggacctt aatattgata tagacgttgt
     2341 agggtgtcca attataaggg aagaggatgg actagctaaa agttctagaa acacatactt
     2401 aagtttagag gaaagatctt cagctactat tttaaataaa tctttaactt tagctaagga
     2461 agctttaaat aatggagaga gagacagctt aaaaataatt gagataataa gtaaaaatat
     2521 aaatacttat aatttggcaa agattgatta tgtagaagtt gttgattctt tatcattaca
     2581 aagagtaaat tatattgaaa aatcagtttt agttgcaata gcagtattta taggaaaaac
     2641 aagattaata gataatttta catttgaatt acaatagggg gctataatga aagttacaat
     2701 gttaaaagga aaaatacata gagctacagt tgaacaagct gagttagatt atgtaggaag
     2761 tattactgtt gatatgaatt tattagaagc agcaggaata tatgaatatg aaaaagtaca
     2821 aatagttgat gttaataatg gaaatagatt tgaaacttat acaatagctg gagagagagg
     2881 aacagggatg atttgcttga atggagctgc agctagatgt gtatcaactg gagataaaat
     2941 aattttaatg gcatattgtg aattaaatga aaatgaggtt aaggatcata gacctaaggt
     3001 tgtttttgtc gatgataata ataaagttga aagagtaaca tcatatgaaa aacatggaag
     3061 gttatcagat ataattcttt aggttttgta atgttattta tatatagcta gactattatt
     3121 gaaatattta ttgattacta attggataga tgtcaataaa gtagacacaa aaagagaaga
     3181 tacagactgt agacaaagtc cattttatgt gaatcggcta cactagacta gacagaaaaa
     3241 ataaggtgtg tagaataata aaaaacacac tggaggattt ttcatgtcaa gacgtcaacg
     3301 aagaacctat tcaaaagaat tcaaacaaca aatcgtcaat ctctatctcg ctggtaagcc
     3361 tcgcgcagaa attattcgag agtatgagct tacgccttct tctttcgata aatggatgaa
     3421 gcaagcacaa tcaacgggct cattcaaaaa aagagacaac ttaacaccag aacaagcaga
     3481 attgatcgca ctaagaaaga aaaataagca actcgaaatg gagaatgata ttttaaagca
     3541 agcggcgctg atattcggac gaaaagacaa gtaattgatg ccaacaagca taaatattcc
     3601 atatcagcga tgtgcaaaat tctaaatatt tctcgtcaga cctactatta tcaa
//
LOCUS       DAPBBG010000121         3634 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPBBG010000121 DAPBBG010000000
VERSION     DAPBBG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.128"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            348..431
                     /locus_tag="RD477_002801"
     CDS             348..431
                     /locus_tag="RD477_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDT7635095.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            556..1293
                     /gene="erm(B)"
                     /locus_tag="RD477_002802"
     CDS             556..1293
                     /gene="erm(B)"
                     /locus_tag="RD477_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDT7635096.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1556..2419)
                     /locus_tag="RD477_002803"
     CDS             complement(1556..2419)
                     /locus_tag="RD477_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7635097.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNLIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(2421..2693)
                     /locus_tag="RD477_002804"
     CDS             complement(2421..2693)
                     /locus_tag="RD477_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7635098.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(2710..2919)
                     /locus_tag="RD477_002805"
     CDS             complement(2710..2919)
                     /locus_tag="RD477_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDT7635099.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2989..3573)
                     /locus_tag="RD477_002806"
     CDS             complement(2989..3573)
                     /locus_tag="RD477_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7635100.1"
                     /translation="MASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLL
                     APLKSDYDLIIIDTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQE
                     QFNPGLDMIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNG
                     ITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1115 a          611 c          591 g         1317 t
ORIGIN      
        1 tttgtacatt taacttgacg gtgacatctc tctattgtga gttattagtg gtacagtttt
       61 caaccgtttt aattataaaa aagtggtgca tttttaaatt ggcacaaaca ggtaacggtt
      121 attgcaggtg tatttcttat ctatgggttt aacatggatt ttatcattaa aatcatgagt
      181 attgtccgag agtgattggt cttgcgtatg gttaacccta aagttatgga aataagactt
      241 agaagcaaac ttaagagtgt gttgatagtg cattaaaaat tttgtataat aggaattgaa
      301 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
      361 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
      421 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      481 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
      541 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      601 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      661 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      721 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
      781 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      841 aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      901 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      961 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     1021 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     1081 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     1141 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     1201 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     1261 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     1321 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     1381 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     1441 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     1501 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     1561 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     1621 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     1681 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     1741 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     1801 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     1861 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     1921 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     1981 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     2041 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     2101 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     2161 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     2221 cattatcaat gacaataaga ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     2281 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     2341 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     2401 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     2461 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     2521 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     2581 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     2641 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     2701 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     2761 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     2821 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     2881 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     2941 tcttcttact cttattttat catcaacaat cacttgtgat ttgtgattct attctttttc
     3001 gttttctaat tgaataattc gctcaagcat ttcaaaaaat acgttcttat acatggataa
     3061 aacttttttg tcatagcctt tatgctctgt aatgccgttt ttagaccagg tacttacttt
     3121 attacttcgc ttgataatat tttggaaaac caagttatct tctttatgtt gcttatacag
     3181 ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag gaacaaaacc
     3241 gatcatatct agtccagggt taaattgttc ttgtaaatca atcaaatagg aaatatagtt
     3301 ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt aatcactcgc
     3361 cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa taatgagatc
     3421 atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcgt tttcaaacgt
     3481 ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggaa tcaagtctaa
     3541 attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcagtc cttcataaaa
     3601 attgacacgt ggcaattcta ccttaaatgt tttt
//
LOCUS       DAPBBG010000122         3557 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPBBG010000122 DAPBBG010000000
VERSION     DAPBBG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.129"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            3..1193
                     /locus_tag="RD477_002807"
     CDS             3..1193
                     /locus_tag="RD477_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDT7635101.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            1297..2052
                     /locus_tag="RD477_002808"
     CDS             1297..2052
                     /locus_tag="RD477_002808"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDT7635102.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            2168..3466
                     /gene="eno"
                     /locus_tag="RD477_002809"
     CDS             2168..3466
                     /gene="eno"
                     /locus_tag="RD477_002809"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDT7635103.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         1238 a          675 c          744 g          900 t
ORIGIN      
        1 aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg
       61 ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt atcaaagcag
      121 cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt ttctctcacc
      181 ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca gttgcaaaac
      241 gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat
      301 tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa aatacacgtt
      361 ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt aaatactggg
      421 cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt gcacatgctt
      481 ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg atggaaaaag
      541 aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg cccaatgatt gcaatccttg
      601 gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct aaagcagata
      661 aaatcttaat cggtggcgga atgacttaca cattctacga agcaaaagga atcaagatcg
      721 gtaactctct agtagaagct gataaagtag aactagcaaa agaattaatc gaaaaagcag
      781 gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca aatgatgttg
      841 aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac atcggcccag
      901 catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt tggaacggac
      961 ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa
     1021 tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagct gcagcagcag
     1081 aacaattagg attcgctgac aaattcacac acatctcaac aggtggtggc gcaagcttgg
     1141 aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa taaacgacca
     1201 agtttgctca attctgacga gtaatcgatc aaaaggtgaa gggagttttc atgactccct
     1261 agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt
     1321 aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac
     1381 aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt attcttacaa
     1441 gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg
     1501 gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga tctaggtgtt
     1561 cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac agacgaagac
     1621 atcaataaaa aagcaaaagc tatctttgca aatggtatga caccaatcct atgctgcggc
     1681 gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg acaaattaca
     1741 gctggtttag ctgatctttc agctgaacaa gtgtcaaaca tggttattgc ttatgaacca
     1801 atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga aatttgtgga
     1861 gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc agttcgtatc
     1921 caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa agaaaatgtt
     1981 gacggtgctt tagtcggtgg cgcaagcttg caagcagatt cattcttgtc attgttagac
     2041 gctgtaaaat aattgttgaa aatttgtgaa aaatgcaaga aatgtttgca ctaatgagca
     2101 aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact caaaggagag
     2161 acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact cacgtggtaa
     2221 cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg gaatggttcc
     2281 ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag acaaagctcg
     2341 ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca ttgctgaagc
     2401 aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga tcgctttaga
     2461 cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt
     2521 tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag gcggattcaa
     2581 cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac atgctgataa
     2641 ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat tcaaagaagc
     2701 tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag ctcgcggatt
     2761 agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa acgaagaagg
     2821 ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta aagacgttgt
     2881 tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact
     2941 agctgactca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct acgaagaatt
     3001 agtttctaaa tatccaatca tctcaatcga agatggatta gacgaaaacg actgggacgg
     3061 attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg acgacttgtt
     3121 cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta actcaatcct
     3181 tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg aaatggctaa
     3241 agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag attcaacaat
     3301 ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa actggttctc tatcacgtac
     3361 tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg gtgaagttgc
     3421 agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct tttctaatga
     3481 agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa gtaatcgaac
     3541 tctctacttg ctgaaat
//
LOCUS       DAPBBG010000123         3462 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPBBG010000123 DAPBBG010000000
VERSION     DAPBBG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.130"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            238..1629
                     /locus_tag="RD477_002810"
     CDS             238..1629
                     /locus_tag="RD477_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDT7635104.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1667..2497
                     /locus_tag="RD477_002811"
     CDS             1667..2497
                     /locus_tag="RD477_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7635105.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2469..3458
                     /locus_tag="RD477_002812"
     CDS             2469..3458
                     /locus_tag="RD477_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7635106.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
BASE COUNT         1238 a          475 c          652 g         1097 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaagtattc cttactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc ctcacaagga
     1561 ctagatgaag atgaaaatat cagttcatta gcagaaagtg catttccaaa agaaaaaacg
     1621 tatcgttaga ttggaaaaat tttgagcaaa gaagtaggag agaacattgg aaaataaaaa
     1681 tataagtata ctgctttctt tgtatgataa agaaaagccg gaatatttaa aggaagcatt
     1741 agctagtatc tttgagcaaa cggtgatgcc ggcagaaatc gtattggttt atgatggccc
     1801 tataaatgag aagcttcagg cagttgtcac ttatttccag caaaaagttc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ctggattaga
     1921 acatactaaa aatcgcttag tggccagaat ggatacagat gacatcatgg aaccacacag
     1981 attggaaaaa caattagaga tcttccaaga atatccagaa atagcgattg ttgggagtaa
     2041 tattgaagaa tttactggca cacttagcaa tgttatcgga agaaggattg tacctgagta
     2101 taatgatgaa atccgtgtgt tttcgaaaag aagaaaccct tttaatcata tgacagtcat
     2161 gtttgataaa aatgctattt tagaagtggg aaactatcgc ccattaattg gatttgaaga
     2221 ttattatttg tgggtaagac tattgaaggc gggatataaa ggatataata tccaagagat
     2281 tctagtacat gctagagcag gtgctgacat gtatgctaga cgtggaggaa gtaaatatct
     2341 tttaccagga attaaaggcc gatatcgcgt atggaaagat ggtctaggat cactaaaaga
     2401 ttttctgata gttatttgcg gacatcttgt aattagttta ttaccgaata aaattagggg
     2461 aaaagtatat gaatcaaaac ttagaaaaac aaactaaact ttctataatc ataccttttt
     2521 acaatgcaaa gaatacttta agaagagctg tagacagcgt cttaacgcaa ggttatccca
     2581 atattgaaat cttgttaatt gatgatggat ctacagatgg tagtagtgac attggtgacg
     2641 aaattgctaa tctagagagt aaagtagcat gttatcactt ggaaaacgct ggtccatcag
     2701 ttgctaggaa ctatgggata aaagaagcaa ctggaaattt ggtaggattt ttagatagtg
     2761 acgattattt tttacctgat tgttttaata atgttttgaa agactaccat aggaatcacc
     2821 ccgatatcat tgcttttgga ttaagtaaag gaaaaaatat agaaaatgca aatttatttg
     2881 ttcccgaaaa gaagattggt tgttcaagtg aagaagcaat tgagcaaatg tttagaagta
     2941 aggcagtgga cttttacgta tggaataaat tttttaaaag ggaattattc aatcatatag
     3001 tttttccaga aaataaatta tatgaggata tggttcctat gtatgaagct tttagggcgg
     3061 ctagtaaaat tgatattttg ccattttcag gtatttttta ttatcaaaat aacgatagca
     3121 tcgtttatca gtcttttaat tcaaaacagt atgataatat tttgcagaga aaaatattag
     3181 ttgatgaagt aaagaaagat tatcctcgac ttattaattt ggccaatgct cggttagtag
     3241 acggatatct ttcaacaggg tttaaaatta ctggcaaaaa tcaaaatcag aaagaaaaac
     3301 aaaaatatta taatcagtct aaaagagaaa taaaaagtat atattcaaaa ataagaagta
     3361 acaatgagat atcaattgct aaaaaattag ctttattttt atacttaatt aatcctaaaa
     3421 cttattttat tttatataaa aaagttctaa aaaaataaag tt
//
LOCUS       DAPBBG010000124         3431 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPBBG010000124 DAPBBG010000000
VERSION     DAPBBG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.131"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..730)
                     /locus_tag="RD477_002813"
     CDS             complement(<1..730)
                     /locus_tag="RD477_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HDT7635107.1"
                     /translation="MAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYMKMLGTPDDDVKILFTKGFVFFLNEDLLTFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPVKLDVILKSGQVEPLRGDLMEKWYPAGIPSDVKSDTQLSQGQDSQDQ
                     TNQDKDSIEEINQDQNSFFENIINQKGIEIITTLGDRYTEVQREKYLKLIASMKDSIE
                     REYKIEIRGQDYFDITNKMLTRLFN"
     gene            complement(759..960)
                     /locus_tag="RD477_002814"
                     /pseudo
     CDS             complement(759..960)
                     /locus_tag="RD477_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1005..1277)
                     /locus_tag="RD477_002815"
     CDS             complement(1005..1277)
                     /locus_tag="RD477_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7635108.1"
                     /translation="MLEVILPPDLDRELRNYFYHVAREAVDQVRIETSFNKEVFRLGE
                     ACEYLSVSRSTLKKFIREKGLKVSVINDCQFIRKEELARFIKEHEV"
     gene            complement(1291..1491)
                     /locus_tag="RD477_002816"
     CDS             complement(1291..1491)
                     /locus_tag="RD477_002816"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7635109.1"
                     /translation="MLAEIIKIKRIERGLTQEELARIVGISRSYYSDIENNRTIPSGK
                     VLLNLNKTLKFFKINDVNNGIK"
     gene            1608..1796
                     /locus_tag="RD477_002817"
                     /pseudo
     CDS             1608..1796
                     /locus_tag="RD477_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2290..>3431
                     /locus_tag="RD477_002818"
     CDS             2290..>3431
                     /locus_tag="RD477_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7635110.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGINPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKQDTNETFQEIYELWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGKFRIKEITVKHVQKILNQWAKMTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRNQLEILFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALNWSDIDFDSNTVTINKTLSQTKYGYKISSPKTKNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLN"
BASE COUNT         1207 a          615 c          476 g         1133 t
ORIGIN      
        1 aattaaaaag tcttgttagc attttgtttg ttatatcaaa ataatcttga cctcgaattt
       61 ctattttata ttctctttca attgaatctt tcatactagc aatcaactta agatactttt
      121 ctctttgaac ttctgtatat ctatcaccta gtgtagtgat tatttcaatt cctttctgat
      181 ttataatatt ttcaaaaaag gaattctgat cttgattaat ctcttctata ctatccttat
      241 cttgattagt ttgatcttga ctatcctgac cttgacttaa ctgtgtatcc gatttgacat
      301 cgcttggtat accagctgga taccattttt ccattaaatc gcctcttagc ggttctactt
      361 ggccagattt caaaatcaca tcaagtttta ctggctttaa ttttgagaaa aattctggtt
      421 ttaagttcaa taacttgaag cggtaaatag atggtttata cctatctatt cgtatatttt
      481 ggttttgtaa ccaatggggg agaaatgtta acaaatcttc atttaaaaaa aatacaaaac
      541 cttttgtgaa aagtatcttt acatcatcat cgggtgttcc gagcattttc atgtatggat
      601 acgcttcgac aattccgtca tcgtctgcgt ttgtgttcag atatacgtat aataattttg
      661 ctgaatctga catcttcata aaacttgcat tatttaaaaa tgatttagcc agcattcgtt
      721 tttctgccat aaaattcaca cttcttccta tttgaatgtt aaaatgtagt caaataaagt
      781 tttctcagaa ctttattgtt aagtgctccc ctttgtctgc aaactatcag gatcgctcct
      841 ttttattttc ttcaaagcgt tcttaatacc tttttctgca tattcccgtt gcagaatatc
      901 aaactgcaag tacatctcga tttcttttag aatcaacttt gctacttcat catcgttcaa
      961 acaaacactt cctttttcat atcaaaagca cgccacgcaa ctttttatac ttcatgttct
     1021 ttgataaacc ttgctaattc ttcttttcta ataaattgac agtcattgat aacactaact
     1081 tttaatcctt tttcacgtat aaatttcttt aatgtacttc tgctcacact taaatactca
     1141 cacgcttcgc ccaaccggaa gacctcttta ttaaaagaag tttcaattct aacttgatct
     1201 accgcctctc tagcgacgtg ataaaagtaa tttctcaatt ctctatccaa atcaggtggt
     1261 aaaataactt ccaatattac tcctcctcac ttatttaatt ccgttattta cgtcatttat
     1321 cttaaaaaat tttagggtct tatttaagtt caataaaacc ttccctgacg gaatagtacg
     1381 attattttct atatcagaat aataacttct cgatataccg acaattctag ctagctcttc
     1441 ttgcgtaagc cctcgttcaa tacgctttat ctttataatt tcagctaaca ttacaatccc
     1501 ccttccaatg acgtacattt acgtctaaaa atatcatact gcactgcagt tcctaagtca
     1561 acaaaaatgt tgtgtattta cgtcatttaa catatactac tttatatagt gtggaggtga
     1621 aatgtttgat tgatattgga aacaacatca aaaatattag aaaaagtaaa aaaatgacac
     1681 aaactgattt tgctgagtta atgggtctat cacgatcata tataggggat ttagaaaaca
     1741 acaggagtaa tcccagtata aaaacactgg aagccattgc aagagcattg aatgtcgacc
     1801 tacaaaaaat tattagttct gatcgaactc taaatgccaa agataatacc ctaaaaataa
     1861 tcgagtatac acaattatat aataataaat atttatttaa attggctggt acggtgactc
     1921 ataaatggaa ttttatatat gcaaatgttg atctatcagc aattaatctc aaaaataagt
     1981 actatatttt tattgagtgt catacttccc ctgattcagg agattcatct tcagatttaa
     2041 aaagagcaac agaattcata aaaaaatttt attcttcaaa agcacttcaa aatgtttcaa
     2101 gagaaatatt agaaattatc ttttcagaac aaaaagactt actacttggg attcagacag
     2161 taagaataat ctgggaagga gttcaatact tacctagtga aacattaact atcgatatag
     2221 aaacaaatta gttaaatcag atttatttcc tatcaaaagc acgccacgca actgatagga
     2281 ggaaaaacaa tggcaacaat taaacaatat gaaaagaaag acggaaccaa agcatggttg
     2341 tttcaaactt accttggaat caatccatta actggtaaag aagtccgtac tacacgtaga
     2401 aattttaaaa caaaaaaaga agctcaaatc gagcttaata gactacttgt agactttgaa
     2461 gagaacggtc ttaaacagga tacgaacgag acttttcaag aaatctatga actttggtat
     2521 gaaagctata aaaccactgt aaaggaagtc actcaattaa aaattgagat caagttcaat
     2581 aaatggattc ttccaaaata tggtaaattc aggatcaaag aaatcactgt aaaacacgtt
     2641 cagaaaatat tgaatcaatg ggcgaaaatg acagatcaat ataaagtcct acattctact
     2701 gtaagccgta ttttcaaata cgcaatgact ttggggctaa ttactaaaaa cccctgtgaa
     2761 cttgtaatca tgcccaatag aaatgttgaa aaaaagaaag atactgtcaa aatttattca
     2821 cggaatcaac tagaaattct cttccaatac ttggaatcaa aaagaagtac ctatagagca
     2881 gattacgata aaacgctact tcgctttctg ttttttagtg gttgtcgaat tagtgaggct
     2941 ttagctttga actggtcaga tattgacttt gattcaaaca ccgttactat aaacaaaacc
     3001 ttaagtcaaa caaaatatgg atacaaaatt tcaagtccaa aaacgaaaaa tagctatggt
     3061 tctctatctt tagacaataa aaccattaat tggctaaaga aatggcaaat caatcaaaaa
     3121 agatatatgt taacccttgg tattactgat ccaacaatga ttttttgtgg aatctacaag
     3181 gaaatcatta ctaaacacgc tatttattct cgtatgatca caatctgcaa gaattctggt
     3241 attccatttc taggaaacca tgtaaccaga cacactcatg ctagtatgtt gttagagtca
     3301 ggagtttcta tgaaggaagt tcaagaacgc cttagacact catcaatcaa attaacaatg
     3361 gatacttatg ctcatctaac taaagaaacc aaagaaaaaa ccgttgaaaa gcttgcagaa
     3421 cacctaaatt t
//
LOCUS       DAPBBG010000125         3428 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPBBG010000125 DAPBBG010000000
VERSION     DAPBBG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.132"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..411
                     /locus_tag="RD477_002819"
     CDS             <1..411
                     /locus_tag="RD477_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635111.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            521..571
                     /locus_tag="RD477_002820"
                     /pseudo
     CDS             521..571
                     /locus_tag="RD477_002820"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            605..1981
                     /gene="tet(L)"
                     /locus_tag="RD477_002821"
     CDS             605..1981
                     /gene="tet(L)"
                     /locus_tag="RD477_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7635112.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            2576..2887
                     /locus_tag="RD477_002822"
                     /pseudo
     CDS             2576..2887
                     /locus_tag="RD477_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            3022..>3428
                     /locus_tag="RD477_002823"
     CDS             3022..>3428
                     /locus_tag="RD477_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HDT7635113.1"
                     /translation="MTFNIINLETWDRKEYFNHYFNQQTTYSVTKEFDITLLKSMIKN
                     KGYELYPALIYTIVNIINQNKVFRTGINSEGNLGYWEKLNPLYTVFNKETEKFSNIWT
                     ESNVSFNSFYNSYKSDLLEYKDKNEMFPKKPIP"
BASE COUNT         1137 a          470 c          641 g         1180 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgaaat gaatgtaaca gggttcaatt
      541 aaaagaggga agcgtatcat taaccctata aactacgtct gccctcatta ttggagggtg
      601 aaatgtgaat acatcctatt cacaatcgaa tttacgacac aaccaaattt taatttggct
      661 ttgcatttta tcttttttta gcgtattaaa tgaaatggtt ttgaacgtct cattacctga
      721 tattgcaaat gattttaata aaccacctgc gagtacaaac tgggtgaaca cagcctttat
      781 gttaaccttt tccattggaa cagctgtata tggaaagcta tctgatcaat taggcatcaa
      841 aaggttactc ctatttggaa ttataataaa ttgtttcggg tcggtaattg ggtttgttgg
      901 ccattctttc ttttccttac ttattatggc tcgttttatt caaggggctg gtgcagctgc
      961 atttccagca ctcgtaatgg ttgtagttgc gcgctatatt ccaaaggaaa ataggggtaa
     1021 agcatttggt cttattggat cgatagtagc catgggagaa ggagtcggtc cagcgattgg
     1081 tggaatgata gcccattata ttcattggtc ctatcttcta ctcattccta tgataacaat
     1141 tatcactgtt ccgtttctta tgaaattatt aaagaaagaa gtaaggataa aaggtcattt
     1201 tgatatcaaa ggaattatac taatgtctgt aggcattgta ttttttatgt tgtttacaac
     1261 atcatatagc atttcttttc ttatcgttag cgtgctgtca ttcctgatat ttgtaaaaca
     1321 tatcaggaaa gtaacagatc cttttgttga tcccggatta gggaaaaata taccttttat
     1381 gattggagtt ctttgtgggg gaattatatt tggaacagta gcagggtttg tctctatggt
     1441 tccttatatg atgaaagatg ttcaccagct aagtactgcc gaaatcggaa gtgtaattat
     1501 tttccctgga acaatgagtg tcattatttt cggctacatt ggtgggatac ttgttgatag
     1561 aagaggtcct ttatacgtgt taaacatcgg agttacattt ctttctgtta gctttttaac
     1621 tgcttccttt cttttagaaa caacatcatg gttcatgaca attataatcg tatttgtttt
     1681 aggtgggctt tcgttcacca aaacagttat atcaacaatt gtttcaagta gcttgaaaca
     1741 gcaggaagct ggtgctggaa tgagtttgct taactttacc agctttttat cagagggaac
     1801 aggtattgca attgtaggtg gtttattatc cataccctta cttgatcaaa ggttgttacc
     1861 tatggaagtt gatcagtcaa cttatctgta tagtaatttg ttattacttt tttcaggaat
     1921 cattgtcatt agttggctgg ttaccttgaa tgtatataaa cattctcaaa gggatttcta
     1981 aatcgttaag ggatcaactt tgggagagag ttcaaaattg atcctttttt tataacagga
     2041 attcaaatct ttttgttcca ttaaagggcg cgattgctga ataaaagata cgagagacct
     2101 ctcttgtatc ttttttattt tgagtggttt tgtccgttac actagaaaac cgaaagacaa
     2161 taaaaatttt attcttgctg agtctggctt tcggtaagct agacaaaacg gacaaaataa
     2221 aaattggcaa gggtttaaag gtggagattt tttgagtgat cttctcaaaa aatactacct
     2281 gtcccttgct gatttttaaa cgagcacgag agcaaaaccc ccctttgctg aggtggcaga
     2341 gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga aaggtcttaa aggttttatg
     2401 gttttggtcg gcactgccga cagcctcgca gagcccacct ttacgaagta aagtatagtg
     2461 tgttatactt tacatggaag ttgtaccgaa atattgtgtt gtgaagtcta tataatttta
     2521 aaattatgaa gtctatatac gtgcgtaaag atatttaaag agattaaaaa taataaaaat
     2581 aaaaattgaa ttaaaaagag atatggttga ttattggaac gattgtttta atgatttaca
     2641 tatattacaa ccagattgga aaactatcga acgtacttct gatagagcaa tggtttttat
     2701 gttgttgaat gatgaagaag aatggggaaa attagaaaga cgtactaaga ataaatataa
     2761 aaaattaatt aaagaaatat ctctaattga tttaactgat ttaatgaaat cgactttaaa
     2821 agcgaacgaa aaacaattgc aaaagcagat tgatttttgg caacgtgaat ttagattttg
     2881 gaagtaaagt aactttgttt tatacaaaat gttactttag tataattaac gtttaagagg
     2941 tgacacaata tatgaaaaaa tcagaggatt attcctccta aatacaaaga gataagatga
     3001 aatttaggag gaaaattata tatgactttt aatattatta atttggaaac ttgggataga
     3061 aaagaatatt ttaatcatta tttcaatcaa caaacaactt acagtgttac taaagaattt
     3121 gatatcactt tacttaaaag tatgataaaa aataaaggat atgaactgta tcctgctttg
     3181 atttatacaa ttgtaaatat tataaatcaa aataaagtat ttagaacagg aattaatagt
     3241 gagggaaatt tgggttattg ggaaaaatta aaccctttat atacagtctt taataaagaa
     3301 actgaaaaat tttctaacat ttggacagaa tcaaatgtta gttttaattc tttttataat
     3361 agttataaga gtgacttact tgaatataaa gataaaaatg aaatgtttcc taaaaaacca
     3421 atacctga
//
LOCUS       DAPBBG010000126         3419 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPBBG010000126 DAPBBG010000000
VERSION     DAPBBG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.133"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            164..715
                     /locus_tag="RD477_002824"
     CDS             164..715
                     /locus_tag="RD477_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HDT7635114.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1391..1645
                     /locus_tag="RD477_002825"
                     /pseudo
     CDS             1391..1645
                     /locus_tag="RD477_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1715..2227
                     /locus_tag="RD477_002826"
     CDS             1715..2227
                     /locus_tag="RD477_002826"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7635115.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2224..3261
                     /locus_tag="RD477_002827"
     CDS             2224..3261
                     /locus_tag="RD477_002827"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7635116.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1253 a          577 c          678 g          911 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaatt atctaaagag aattaagttt tgattatgta
     1141 ataagttttg gtactaaaaa agacttgatc taattagacc aagtcttttg ttagtgttat
     1201 attaataaca aaataaaaat aagaagtcgc tcactccccg accaaagttt gtgagcaaaa
     1261 taaacagtcg atgtttgttt cttaattgta tatcttgata ttaaacgatt taagattatt
     1321 cttcaagata tatattcgca aatgtaattg gaaaagcgac ttcttaaaca aaattaagga
     1381 gtcgcttttt atggcaaaaa taacgaaaaa tgagcaagaa aatttggtgg aaaattacac
     1441 aaaaaaaccg gcgtactcta atagccggtt agacgcacat ttcgtgtgca cttctaatcc
     1501 gaaattgagc tttgacgcta tgacgatagt cggaaacctt aataaaaaca gcgctaaaaa
     1561 gctatccgat tttatgagtt tagatccaca aattagactt tgggatatac ttcaaacaaa
     1621 atttaaagct aaagcactac aagaccccct atgttaggct agttgtcgta taggataata
     1681 agttataata aagactatac ggaggatgat taccatgaca aataaaaaca atgaacgtta
     1741 ctctaccgaa ctgaaagcga cagccatcaa gaaaatgatg ccacccgaaa atagggggat
     1801 tcgccaatta agtcaaactt tgggcattcc agaagcgact ctttgttctt ggcgtaaaaa
     1861 agctagaatt caagggaata ctacacccgg aacgaaaaaa aatgtcgaaa aatggtcaag
     1921 tgaagataaa tttttaattg tgatggaatc attctcaatg aaccaagtag aacttgcgga
     1981 atattgccga aagaagggct tatttaaaga acaaattgag gcttggaaga aaacgtgctt
     2041 atctgcgaat gaacaggaag aaaatcgaac gagagaactt gcaactgaac taaaagaaga
     2101 gaaaaaacaa gcgcgtcaat tagagaaaga attacgtcat aaggaaaaag cattagcgga
     2161 agccgctgct ctcttattac taagaaaaaa agcacacgcg atttgggggg accaagagga
     2221 agaatgattt catcttcaga tcgcaaacaa gctgttgaac ttattcaaga agccaatcgc
     2281 aatggggccc gtctcaccta tgcctgtaat gagctgaata ttagcgttcg aacctacgaa
     2341 cgatggacgc gtgaaggtac gatagcgcat gatcaacgcc ctttggcgaa acgtcctgta
     2401 cccaaaaata aactgacgga tcaagaacgc gaaaaaataa tagaaaccgt ttcaaaaaaa
     2461 gaatttatga atttgccacc tagccaaatt gtaccaaaac ttgcggattg ctcgatttat
     2521 attgcatcag agtctagctt ctatcgtgta ttgcgagaaa aaaatatgca acatcaccgt
     2581 ggccgaagtc aagtttcaca aaaacgcata ccgccaagtc accttgcgac gaagccaaat
     2641 gaagtttgga cttgggatat tacttggcta aaaggtccaa taaaaggact cttttaccgg
     2701 ttatatctca tcattgattt atttagccgt aaaatggtag gctgggagat ttgggaatca
     2761 gaagaagctc gtcatgccga aaaacttatt aaacagacgg tgcttaagga acgtatccaa
     2821 ggacagccgt tggttttaca ctccgataat ggcagcccta tgaaagccgc gacttttcaa
     2881 gtcttactcg aaaaattagg aattcaaagt tcttattcaa gaccacgagt gagtaatgac
     2941 aatccttatt cggaagcggc tttccgaacg ttaaaatatc gaccagaata tcccaataac
     3001 ggctttaaaa caatagatga tgctcgaaaa tggacgaaag agtttgtcga ttggtataac
     3061 gattctcatt tgcatagtgg gattcaattt gtgtctcctg cagcgtgtca tgaaggacga
     3121 catctcgcta ttttagagaa acgaaagcag gtttacgagg acgctaaaaa gcgccatcct
     3181 gaacgctggt caaaaggtgt tcgaaattgg actccacatc aatcggtggc gttgaatcct
     3241 ttaaaagaaa cagaaagtta acaggagcat aatctttcaa gattatcaaa taaccctcgt
     3301 cacccaatag gggaaaaagt tattcttccc ctattggata acgaggccaa ccgaccatag
     3361 ggaggttctg ctttttgata ggtggaaaat aaaaatacga caactatatt gacaaacac
//
LOCUS       DAPBBG010000127         3302 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPBBG010000127 DAPBBG010000000
VERSION     DAPBBG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.134"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(369..1487)
                     /locus_tag="RD477_002828"
     CDS             complement(369..1487)
                     /locus_tag="RD477_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635117.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1958..3265
                     /locus_tag="RD477_002829"
     CDS             1958..3265
                     /locus_tag="RD477_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDT7635118.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1038 a          684 c          593 g          987 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 at
//
LOCUS       DAPBBG010000128         3245 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPBBG010000128 DAPBBG010000000
VERSION     DAPBBG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.135"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..209)
                     /locus_tag="RD477_002830"
     CDS             complement(<1..209)
                     /locus_tag="RD477_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7635119.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="RD477_002831"
     CDS             complement(308..622)
                     /locus_tag="RD477_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635120.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="RD477_002832"
     CDS             complement(690..1184)
                     /locus_tag="RD477_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635121.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1177..1491)
                     /locus_tag="RD477_002833"
     CDS             complement(1177..1491)
                     /locus_tag="RD477_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635122.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1707..3098)
                     /locus_tag="RD477_002834"
     CDS             complement(1707..3098)
                     /locus_tag="RD477_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDT7635123.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
BASE COUNT          954 a          605 c          434 g         1252 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaat tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttacccg
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa
     1681 actggaaagt ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt
     1741 tgctgatttt caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt
     1801 acatcgtcat tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga
     1861 gaacaatttt caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata
     1921 gaaattgttt tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta
     1981 atcgtgtatt cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct
     2041 cgcaagaagt catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt
     2101 tgtgcgatgg aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact
     2161 ccactaatta gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat
     2221 tcttctaaat tcttttcaag ttgttcagaa acagattcaa atgaatatct tttccctaac
     2281 tttcttgcac taaaagtacg ttcgacatac tgactttttt cttttctaga ttgtttatct
     2341 cttcctaaag taattaactc acctctacgc tcatagaaat ttacaccttg tttgtaaaga
     2401 acatagccaa gtttgttatt tgtttttgaa aattgataaa cttcaatgtt atattctttt
     2461 gccaaatgat ccgaaaaatc ttcaaaagaa ttcacttcgc atttttccaa cgttgcttcg
     2521 atcgcatttt gcacaaactc ttgataatta atttttccat atttcatctt agaaggatcg
     2581 tttgaataaa gattggttct cttttctaag ttttcatagt actcattatt cactgattca
     2641 agaccatgtt cattgaggac ttgatcgtta atttttcgca ttagttctaa atctttaggg
     2701 tcaatacgca accttttaca tgtttccatg ttaatattat taatgacaag atgactatga
     2761 aaatgatctt tatcattgtg acttgcaaca atgatttgcg aatttggaaa agctttctct
     2821 gcaaattcga cagctatttc gtgcatttta tcaatactta actctttaga agtactctta
     2881 tcaaacgaat gaataacatg aaacgcttgt gctatatctt tagatttatt catcttggta
     2941 cgtactaaat tcatttcttg cagtgcttcc tcactatcat cactacaatt tattcctgag
     3001 actgcaacac atttttcttt ttttgaagga ttagctgcat attgtaatag cgaggataaa
     3061 tttttcttgc gtcctttcga tgttatagta gctcccaaag ttgtttcacc tcatctttta
     3121 aacttgcaat aatctgaatt gagtgaatta aattccgatc aactttttgg gaatactctt
     3181 ctttcgtacc cggtctagtt tgcttttcta tccaatattc gaaatattct ttttctactt
     3241 ctgat
//
LOCUS       DAPBBG010000129         3182 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPBBG010000129 DAPBBG010000000
VERSION     DAPBBG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.136"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            119..3151
                     /locus_tag="RD477_002835"
     CDS             119..3151
                     /locus_tag="RD477_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDT7635124.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTAVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
BASE COUNT         1073 a          522 c          686 g          901 t
ORIGIN      
        1 tataataaat caaatgattg ataataagat agctgaattg atggaacttg agagttataa
       61 aaaagctctc atctacgaat acgtaaccgg taaaaaagaa gcctaggagg tgattcattt
      121 ggtagagaac aaagaaattc gttttgagca ggatattgag acttcactga ctacgtatgg
      181 caaatggaag aaagaaacat tcaatgaatc aaactttgat cccataattg gtttggattg
      241 tcaaaaattg ctaggctttg tccagaagac tcaaccaaaa cagtgggcac gtcaccaacg
      301 aaactataac aatgatggag aagtcaagtt ccttcaacgc tttaatcaag aggtagaaac
      361 ccacggcatg ttacatgtat tgcgcaatgg gatcaaagac cgcggtgtgc gctttaaagt
      421 agcttacttc aaacctgctt cagagctgaa tgatgaaacg atggaaaagt atgaagcaaa
      481 tagttttacc tgtactcgcc agtttgctta cagtcctaat aaccataaaa ccattgatat
      541 ggttttgtcg ttaaatggta ttccgctcgt tgctttagaa ttgaaaaacc agtacactgg
      601 tcaaacagta gaaaatgcca aactccagtt tcaaaaagat cgtgatccaa aagaattggt
      661 atttcgtttt aatcgccgct tcttagtcta ttttgcggtg gaccatacag ctgtttatat
      721 gacgacaaag ttggacggta aaaaaacgta cttcttgcca ttcaatcaag gttccaatgg
      781 gccgggaaga gttggtggag caggcaatcc gtccaataaa gatggctacc caactgccta
      841 cttgtgggaa aaggtgctta ctagagacaa tttgatggat attatccaac gttttatgaa
      901 tctagagatt aaaaaagaaa agcaaggcga cagagaagtc acaaagaaaa cgttgatttt
      961 tcctcgttat catcagttag atgtggttcg aaaattggtt gcagatgtaa aaagccatgg
     1021 tagtggagaa aattatttga tccagcatag tgctggttct ggaaaatcta atagtattgc
     1081 atggctggca taccatttag cctctattca tgatcaactg aactacccaa ttttttcatc
     1141 aattattatt gtgactgatc gaacagtgtt ggatcgccag ttgcaaaata ctatcatgaa
     1201 ctttgatcat cagacaggtt tggtagaaac gattgacgat cgcaaatcct ctcaagattt
     1261 aaaagaagcc attaatgatg ggcgtaagat cattattacc actttgcaaa agttcccagt
     1321 gatctatcag gaagtagatc aagtggaagg tcgtaatttt gctgtgattg tggatgaggc
     1381 acattcttca caaactggga acagtgccca gaagctaaaa gccgctcttt cggacaaatc
     1441 taaatccttg gaagagtatt atgaactgga aggcgaagtt gacgatgtat taccagatgc
     1501 tgaagatgaa ctagttcaac aattggtagc acaaggtaaa catcaaaatt taagtttctt
     1561 tgcttttacc gctacaccga aagaaaagac attggaaatg tttggtacga aacaaccaga
     1621 tgggcatttc gaaccctttc atgtttactc gatgcgccag gcgattgagg aaggctttat
     1681 cttagatgtg ctgaagaact atatgaccta tagtacttcc tacaaaattg cccgtgaaat
     1741 tgaagataac ccagaattac caaagagtga agcaacccga gcgattgttc gtttccaatc
     1801 attgcatcct tgggcagtta ctcaaaagac ggctgtgatg gttgagcaat tccgagcggt
     1861 tactcgtaca gctattggtg ggcaggcaaa agcaatggtc gttaccgcat cacgcttaca
     1921 cgctgttcgc tatatgaaag aatttaaaaa atatatagcg aacaaaggtt ataatgactt
     1981 ggatgtgttg gttgcttttt ctggttcggt agtagatgga gagactgaat atacagaaac
     2041 gaagatgaat gtcacaaaat ctggtgagaa tgttcgggag aatcaattaa aggaagcttt
     2101 ccatacagat aattttaata tcttgattgt tgctgaaaaa tatcaaacgg gttttgatga
     2161 accgttgctg cataccatgt ttgtcgataa gaagttaaag ggtgttaaag ctgttcagac
     2221 tttatcacga ctcaatcgaa caatgcctgg gaagaaggat acctttattc ttgattttgt
     2281 aaatgatgtt gaagatattg aagctgcttt ccaacccttt tatgaacaaa caaatttgag
     2341 agaagaaatc aatgttaact tgatttatga tactcaaagc aagctacgaa aatttaatgt
     2401 ttacaatcaa ggtgatattg aacgtgtcat gaaattggtg aaacaagccc aaaagcaaca
     2461 gaatgagcgc ttgcttggac gaatttctag catgtttaag ccgattatgg atcgctatga
     2521 ggaaatgaac aaggataatc agtaccagtt tcgggtgact ctacgtaact ttggcaagtg
     2581 gtatgattat atttcacaac ttgatcggtt attcgatatg gaactattgg aggaacgaat
     2641 ttttactgga tacttgctga agtttctacc taaagaaaca cgagaaaata ttgatatttc
     2701 tgacaaagta aagttggaat attataagtt acgtcaggac ttttccggtg aaatccatct
     2761 agtgaataaa gaaggcgagc tagtaaatcc aaagtcaatt gatgcaggaa tcaaaccgcc
     2821 ggatgaacga gattcactag aagaaatcat tcataaaatc aatgaacgat ttccaaatga
     2881 ttttgacgaa ggagaccggg tgttagtaga gattttgtat cggtcatttg ccgataaccc
     2941 agatttgaag gttgtcagca tggctaagaa taacgatgct cagatgtttg aaaaatcgct
     3001 gttccctgat gtatttaaag ataaggtgat ggagcagtac gagaagaaca atgcagcttt
     3061 tgagaaaatg tttgggcatg atgataagta ctttgatcta gtatatacat tagttgctag
     3121 agatttatac aagttattac ggacgaaata aaatttataa ataaggatta gacaatacaa
     3181 tt
//
LOCUS       DAPBBG010000130         3051 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPBBG010000130 DAPBBG010000000
VERSION     DAPBBG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.137"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..521)
                     /locus_tag="RD477_002836"
     CDS             complement(<1..521)
                     /locus_tag="RD477_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDT7635125.1"
                     /translation="MENENRTDWTLPRKNLNPKTKQPYKRGRNWWIVVYPESLPENWK
                     EIISTEPVAISPLHDKDVNADGTKKKPHYHIVFNYKGNKSFEQMDEMARVLRAPIPER
                     ISGLTGAVRYLTHMDNPEKYQYDNTEIQVFGGFDLESCLALSTGDKRQALKEMLGFIS
                     DNNIMHLKDFADY"
     gene            complement(645..800)
                     /locus_tag="RD477_002837"
     CDS             complement(645..800)
                     /locus_tag="RD477_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein repA"
                     /protein_id="HDT7635126.1"
                     /translation="MVEVEKKKVTLSLPVESNDKLEKMAQKYGMTKSGLVTFLINQAD
                     DKGTIFK"
     gene            complement(1344..2555)
                     /locus_tag="RD477_002838"
     CDS             complement(1344..2555)
                     /locus_tag="RD477_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDT7635127.1"
                     /translation="MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNY
                     DLVNRTENYKRDIEQFINDNKSSSRAVRKDAVLINEWIITSDNPFFKDKDDKEIKDFF
                     DTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFNDEHKLSAKTVFNRQALQAVQ
                     DELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKSEVLALSND
                     KAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVI
                     KSYEELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEK
                     ENKSLKNEIKGIYKGFNQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNE
                     TKTRDRDELSR"
     gene            complement(2808..>3051)
                     /locus_tag="RD477_002839"
     CDS             complement(2808..>3051)
                     /locus_tag="RD477_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HDT7635128.1"
                     /translation="ENTVPISMIPWIDFSSFNLNIGNNSRFLLPIITIGKFYSKNNKI
                     YLPVSLQVHHAVCDGYHVSLFMSEFQNIVDSVNEWI"
BASE COUNT          904 a          631 c          407 g         1109 t
ORIGIN      
        1 caataatcag caaaatcttt taaatgcata atattgttat ccgaaataaa accaagcatt
       61 tcttttaacg cttgtctttt atcaccagta gataacgcta aacaactttc aaggtcgaat
      121 ccaccaaaca cttgtatttc tgtattatcg tattgatact tttcaggatt atccatatgc
      181 gttaaatatc taacagcacc agttaaacca cttattcttt caggaatcgg tgcccttaaa
      241 actcttgcca tttcgtccat ttgttcaaat gatttattgc ccttgtaatt aaaaactata
      301 tgataatgcg gctttttctt tgttccgtct gcattaacat ctttatcatg taatggacta
      361 attgccacag gttcagttga aataatctct ttccaattct ctggcaaact ttcaggataa
      421 acaacaatcc accagttacg acctcgctta taaggctgtt tagtttttgg atttaaattc
      481 tttctaggca atgtccaatc tgttctattt tcattttcca tatttttcta caccttccta
      541 gttactctac taggaaaaca agggaaaatt tgataaactt gagttgcgaa atcaaagaaa
      601 accaaatctt gcgagccctt gctatcttcc tagcagggct ttttttattt aaaaatagtc
      661 cccttatcat cagcttgatt aattaaaaat gtaaccaaac ctgatttagt cattccatat
      721 ttttgtgcca ttttttctag tttgtcattc gattcaacag gtaaagataa agtcactttt
      781 ttcttctcaa cttcaaccac aaaatgcacc tcaattctct tcaaaatctc ttttacactg
      841 taattatatt atacatacga caggaataca agattttaca aaaaactttt tttcacctcg
      901 aaataggtgc ttaaaacgtt catataaagg tatttacaag acatttaatc tttttatcta
      961 tttggacaat ggacaatgga cacatggggg gtgtcgtagt gcccccatgt tttttcgctc
     1021 caaaaattcc catgtttttt cgccccctgc tcccactaaa tttccaaata ataaaaccgt
     1081 gggctattcg cccacaccca acaagcgcaa agcgcttgac cttgcctctg aaaagcaact
     1141 tgataactgc attcgtttct ttccgtcgtc ttagcaaaag ctaaacaact caaaaaaaga
     1201 gatcaagggt aaatacactc actacgttcg cccttgacct cttttatttc gctgtttttt
     1261 acgcatttgc cgacgaaaag taaacaatat gcagaattac cctagttttg ataaaaaagc
     1321 gcttagaacg caaatatgag cttctaacga cttaattcat ccctatctcg tgttttagtt
     1381 tcgtttctat gaattttttc aaactctcca ccttttacaa actctttaac ttcgctcata
     1441 aggttattca tcagcttttt agcttgtcca acagttgcac caagggtatt ttccataaac
     1501 tggttaaaac ctttgtaaat gcccttaatt tcgtttttaa gcgacttatt ctctttctct
     1561 aattcttcaa gtgtttcaat attctgatta ctcttacgta ccaacctatt gtaatctttc
     1621 gctaattcgc gatatttctc tttaactcta gtgatttctt taggtaaatc agtttccgca
     1681 taatttttta gcttttcatt tgctttatat agctcttcat agcttttaat cactgcattt
     1741 aatttacttt cagggataat tacatttccc gtttttcgtt cttcattttt cataaattca
     1801 tgtcgcagct taccaatccc aatggtttcg ccacttggta ctttgaccgt tttcgtttcg
     1861 tcttttaaat ctaaactttc tttcttgatt gttggtgctt tatcgttcga taacgctaaa
     1921 acttctgact ttcgctgttc cagcgtggta gttatctctt ttaattcgtc ctttgccttt
     1981 ttatattcag gcaccgttaa atttttacgc tctgaccctt tttcgccacg ttcaagctca
     2041 aaaccacgct catttaaata ctttggcaat tcgtcttgta ccgcttgtaa ggcttgacga
     2101 ttaaaaacag tctttgctga tagcttgtgt tcgtcattaa acggcacaat tcctaagtgc
     2161 atatgcggtg tcgtttcgtc taagtgaact tgagcgtagc ggatattatt atcgccaaat
     2221 ttgtccgcaa aatatgattt cgcagtatca aaaaaatctt taatttcttt atcgtcttta
     2281 tctttaaaaa atgggttgtc agaagttatg atccattcgt ttattaatac agcatctttt
     2341 ctgacagcac gactacttga tttattgtcg ttaataaatt gctcaatatc tcgtttataa
     2401 ttttctgtcc gattgaccaa atcataattt aaatgcgacc gttcaacatc aatatctttg
     2461 ttggaatgat tgtctgtatt tctctgatta tgattaccta ccccgactaa atttcctgat
     2521 tttaccttct gcattctcgc cactgcaaat gacatgtaaa cactcctaac ttcggcacaa
     2581 cttccatgta aagtataacc cactagactt atttactacg tttcataagt cattaatgcg
     2641 tgtgctctgc gaggctagtt tttgtgcaag cacaaaaaat ggactgaata aatcagtcca
     2701 taagttcaaa accaaattca aaatcaaaac accagcaacc aaaaatggta gttgctataa
     2761 acttataaat atcataatta cttaagttta tgaaagttat tttagattta aatccattca
     2821 tttacactat caactatatt ttgaaattca ctcataaata atgaaacatg gtaaccatca
     2881 cataccgcat gatgaacttg caatgagact ggtaaataga tcttattatt cttactataa
     2941 aatttaccta ttgtaataat tggcaatagg aatctactat tattaccaat atttaaatta
     3001 aatgaactaa aatcaatcca aggaatcatc gaaataggaa ctgtgttttc a
//
LOCUS       DAPBBG010000131         3042 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPBBG010000131 DAPBBG010000000
VERSION     DAPBBG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.138"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            255..1223
                     /locus_tag="RD477_002840"
     CDS             255..1223
                     /locus_tag="RD477_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDT7635129.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1216..2247
                     /gene="vanA"
                     /locus_tag="RD477_002841"
     CDS             1216..2247
                     /gene="vanA"
                     /locus_tag="RD477_002841"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDT7635130.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2253..2861
                     /locus_tag="RD477_002842"
     CDS             2253..2861
                     /locus_tag="RD477_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDT7635131.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(2950..>3042)
                     /locus_tag="RD477_002843"
     CDS             complement(2950..>3042)
                     /locus_tag="RD477_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7635132.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          900 a          584 c          733 g          825 t
ORIGIN      
        1 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taatttttta
       61 ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa
      121 cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga
      181 gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag
      241 ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg
      301 cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca
      361 acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata
      421 aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt
      481 ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca
      541 ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc
      601 ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt
      661 tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc
      721 agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata
      781 gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg
      841 atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac
      901 aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag
      961 atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg
     1021 tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc
     1081 aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt
     1141 ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg
     1201 aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag
     1261 gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa
     1321 tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct
     1381 tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg
     1441 cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt
     1501 tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc
     1561 ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg
     1621 acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa
     1681 gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca
     1741 ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa
     1801 tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc
     1861 ggttgtgcgg tattgggaaa cagtgccgcg ttagttgttg gcgaggtgga ccaaatcagg
     1921 ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac
     1981 gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg
     2041 gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta
     2101 caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac
     2161 agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc
     2221 ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg
     2281 aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa
     2341 aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc
     2401 ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt
     2461 accgtcctaa gcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc
     2521 tgacaaagga aagttattat cccaatattg accgaactga gatgatttca aaaggatacg
     2581 tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag
     2641 acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc
     2701 atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca
     2761 tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg
     2821 aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg
     2881 acaaactata taagcggttc tgttgcaaag ttttaaataa agaataaaat cccttacggt
     2941 atctatgatt taagctggga ttcccaataa taccttgatt tcagtacaga ccgaaaaccc
     3001 gaagagagtg ccttcttttc gggttttctt atataatcct cg
//
LOCUS       DAPBBG010000132         2866 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPBBG010000132 DAPBBG010000000
VERSION     DAPBBG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.140"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            95..1054
                     /locus_tag="RD477_002844"
     CDS             95..1054
                     /locus_tag="RD477_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HDT7635133.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="RD477_002845"
     CDS             complement(1487..2059)
                     /locus_tag="RD477_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7635134.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="RD477_002846"
     CDS             complement(2075..2680)
                     /locus_tag="RD477_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDT7635135.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          919 a          581 c          471 g          895 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc ctctacaaaa ttcacaatag atataatcta ttgaaaattt
     2821 aaaagaagac aaaatagagt acagaaaatg ttttctgtac tctata
//
LOCUS       DAPBBG010000133         2798 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPBBG010000133 DAPBBG010000000
VERSION     DAPBBG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.141"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            248..532
                     /locus_tag="RD477_002847"
     CDS             248..532
                     /locus_tag="RD477_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635136.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            548..784
                     /locus_tag="RD477_002848"
     CDS             548..784
                     /locus_tag="RD477_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635137.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            806..1060
                     /locus_tag="RD477_002849"
     CDS             806..1060
                     /locus_tag="RD477_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635138.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1142..1327
                     /locus_tag="RD477_002850"
     CDS             1142..1327
                     /locus_tag="RD477_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDT7635139.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1394..1789
                     /locus_tag="RD477_002851"
     CDS             1394..1789
                     /locus_tag="RD477_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7635140.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1779..2156
                     /locus_tag="RD477_002852"
     CDS             1779..2156
                     /locus_tag="RD477_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635141.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2178..2552
                     /locus_tag="RD477_002853"
     CDS             2178..2552
                     /locus_tag="RD477_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635142.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT          988 a          475 c          488 g          847 t
ORIGIN      
        1 attctttact ggaatatcac tgataatttc ctatttgatc tataagaaag ccgaaaatca
       61 cacaagcaaa gcaatcggac tagccgtttc atccttttta actttgctga ctttatttgt
      121 tactttatcg ttttcttttg tttagaacag ctgatccatt agcacctttt tctctttaca
      181 ttatacacac tgttgtgtat aatgtatttg taaacaagct aattaacttt taggaaggag
      241 tgacaagatg ctaacagcta ctttacagca acctactcac gttgctattg cagaaagtta
      301 tacaattaat gcagggatcg ttaaacagct gatcaaggat cgagaattca aaaagtttaa
      361 cagtcggcaa gcgtataaca gacagctaaa gaaagctatt ctaaagcaca aagattcctt
      421 catcgggaac ttggaaagtg tagatgttaa aactgtttca acggcatttt cagttgttga
      481 agtgactatt gacggtcgct tgatttctct atttgctttt ggaattaatt gatagggagt
      541 gtgaactttg gaaggtaaga cttttgcaga aatttggact gaatttattg aggtaccaac
      601 tcgcattgat ccgataactt ctgaaaaggt attggatgca gacttctaca atctcaaagc
      661 aggtactagc cttgaatgcg tttggatgta tttagaagag catttcaaag gcgtaacgct
      721 cggtcacgtc ttataccaca atggtgccga gtgggatgca attggaaata tccatttttc
      781 ttaatataag aaaggagaac acaatatgaa ttataccgat gaagaacaaa tcgctttgga
      841 atacgttttc acaaatgaag aaggggagga aattgaaaaa gtcttgatca tttcatcaaa
      901 agacttggag aaggctttgg cttatgaacc atacgaatca accgtaactg tcattgaatt
      961 cttagatacc tatacttcta atgatacttg ggaaattatg aatatttgca acgctaataa
     1021 cattcaattt gctgaaaaaa ccaataaatc attttattag ttttgcaata aaacatcctc
     1081 tttttctatt gattccatac acaacaatgt gtataataga ttttgtaaag ggagggtagc
     1141 tatgcctaag acagcaagag aaattgtaaa aattctcaaa tctaacggct tcaaaaagac
     1201 tggacaaact ggcagtcatg caaaatttta taattccaag actaaaagaa cggtcgttgt
     1261 tccgatccac aatggtgatg taccactagg aacagaagct aatatctgga agcaagcagg
     1321 gctttaacag ccttgccttc ctatttggta ttaggcatac tatcctctct tacactagaa
     1381 aaggagcgag gaaatgaaga caaaaaatat tttttatccg gcaatcttcg agaaagaagg
     1441 agaagcttat aacatcgttt tccctgactt accggaaata tctacctttg gaaatagtct
     1501 tgaagaagca tatttaaacg ctaaggatgc tttaggatta gcgctatata gtgtaccaga
     1561 tgcagaattt cctaaaccat cagcagtgga agaattaact ttgaaagcca accaagtggt
     1621 agtaatcatc caactaaaca ttaaactttt ccgaagaagc ttaaatacaa aaacaattag
     1681 aaaaaatgtt agtgttccgg aatggttagt tcttttaggg aaggagaaaa acataaactt
     1741 ctctcaacta cttcaaaaag cactagaaga agagttgctt gaaaagtaaa aaggagaccg
     1801 aacctatgaa agctacacac ttaaactatt tctctcgaac gttaatcaat aatctattaa
     1861 aagatacaac tggcattagc gttagtacca aacactttgc caatgatcta attatttttg
     1921 aggataataa cttgcgaatt gagatgttta ttgaacaggc aacccctaac tcaatacctg
     1981 aattctgcta tacgcacact gaaaaaatga aacatgcaaa atcagttaaa gatgttgaag
     2041 cattaagtga atcactaacg aacgctttaa acctcgttaa gaaggcaaaa ttgttatttg
     2101 aatttcaaac cgactaccac aatgagttgc ttgctcaatc tgaaatttta gattaattga
     2161 aacaggagaa gagaaagatg aaacaaacac aaacagaaac cgttaacgat acgcttgctg
     2221 atcaattagc aggttattat aaattatttg agatgggtaa agcacagcgc ttgatccact
     2281 atctttggaa atacaaaatt ttgtttcttt tttgggaatc aacactaaca ttctcagttc
     2341 ttttttataa gtcagatttt tataatcaac acttagtttt agggagtttt ttgcttatct
     2401 acaccatttt gatgcttaat ttatatacct acatcacact tgaagaacac ttagtaatta
     2461 gcgatgcagg gatcgagaga aacatcaaaa gggtatcctt caagatcacg ttaaacaagc
     2521 ctaccgataa aaagctctca gaagcgttct gagagctttt gacattttct tgtataattt
     2581 tatcctaaca cattacaaag cacctaattc gagcttatat caagcaaatt tgctatttat
     2641 tactcaatac atatctaaaa attacttgat ggaaaattag atacatggta agcatgaaaa
     2701 gaaaatcaat ttgagcagat gcagtacaat acatagcaat ttaaaagctc tcagaaggct
     2761 tctgagagct tttaatatat tctttgataa tttgtctt
//
LOCUS       DAPBBG010000134         2761 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPBBG010000134 DAPBBG010000000
VERSION     DAPBBG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.142"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..433)
                     /locus_tag="RD477_002854"
                     /pseudo
     CDS             complement(1..433)
                     /locus_tag="RD477_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(992..1309)
                     /locus_tag="RD477_002855"
     CDS             complement(992..1309)
                     /locus_tag="RD477_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7635143.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1310..1564)
                     /locus_tag="RD477_002856"
     CDS             complement(1310..1564)
                     /locus_tag="RD477_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7635144.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1824..2042)
                     /locus_tag="RD477_002857"
                     /pseudo
     CDS             complement(1824..2042)
                     /locus_tag="RD477_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2602..2688)
                     /locus_tag="RD477_002858"
                     /pseudo
     CDS             complement(2602..2688)
                     /locus_tag="RD477_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          903 a          498 c          398 g          962 t
ORIGIN      
        1 tcatatttcc ttgattacct gaggcaatga aagacttatc tacttgaatt agggtgaaat
       61 tctcattcaa atatggtagt aaaattaata tatgtgaaaa tcgaatcttg agatagctat
      121 tccccttgag atattcactg atacttctac tcaaatcatt taaattttta taattatttg
      181 tacggaacgt atatatatca acatcagttt tctttacatg agaacttatt ctaaaatgaa
      241 aaagcctact ctcagaaaga attgaaaaat agatactttc agtagatact gaagattaaa
      301 caaaattaaa cttgtatcct tcaggcatag cacttttaac agtctgctgc attaactcgg
      361 ataatctttt gaaagtagta atatattttt taccagcaaa ccatacatat cttttttcat
      421 taagtatgtt cactatctct cctccattaa ttatatttag catcaaatag ttattgtacc
      481 tatattttat cccattcctt ttaaaacaag aatacgtaca aagtaaaatg ttgtaaatag
      541 tctagcaggg atgtatattt tcatgtacca acataatatt taagaagttt cccatagata
      601 tattggactt cctcttttaa aacacaaaaa taaatactac cttatttagg tagtatttat
      661 taaaagttac gtcaattttg attaaaaata tactacttga acctctcatg agtaaagtca
      721 cgaaattttt gggaatagag cgtacttgat atcttatttt ttcactaata gcagtgtctc
      781 ttattcacca agcgatcccc gtagttccta cggttcttaa tttttctcta gactaatgat
      841 tgtatcttta gaccttcggc cataacatgg agcaaaataa aaaggtacta agtcttatat
      901 atcaatcttc gagtaaccga cggttaacaa ccatatcgag cggcattaat cacccacctg
      961 tagaggatga gcgacttttg cctaattatt gttaaaagcc tagtttcatt tgtttagatt
     1021 ggaaaatacg tcctacaagt atctcttttt tttcatggtc gatccgataa aataaggcat
     1081 agcttttgcc gatcacgatc cgatatgttg ggatatccat tccatagatt tcggaaactt
     1141 cctcatgcat agttggaaaa tttttggtta gttctaaagc cttctggatc gcacctacaa
     1201 atctttttat gctggctgga gaaagagcaa gctcattttc ccattcttct attatcgttt
     1261 caaggctggt ctggaaactt ttagaataaa ctagcgtgta ctctttcatc tattcactcc
     1321 agccgtattt tccgaaagct tttaccattt caatttctga aactgttaaa cctttctcaa
     1381 agtcttggaa actttcttca atcaatttgc gatcgtgttc ttttgctatt tgagtagtat
     1441 ctaatatgaa taagttgcct tcttgtttgg cttggatagt gtctccgctt tttaatccaa
     1501 gagagcttgg aaggatcact cccacgctat tgccgatctt tctaaccttt agttcttttc
     1561 ccatgttatc acacttcttt tagtttttga taaataaatt ataacatgtt ataacgacaa
     1621 ttgccaacgg tgtttcttaa gggattccac tccatattat tattggacaa aataagccac
     1681 ttagaacgct gaaaacatat acaaacgata tcaaaatacg agtgaactac tcaccataca
     1741 cttccgttaa catccattta agtaggtatt tttattatat atagatttaa catatgtcgg
     1801 tcggttgatt gatatagcta aagttataac tcaaccgaag ccgattcatc tacacgaata
     1861 aagttgcgag tgttctcggc taattcataa aaattgttat gtgttccata tagcaatact
     1921 ggaataactt tattttcttg aatctcataa gcaactctat acacggtgac cgcatactta
     1981 aatccattag accaattacc tgataaacct ccagtttttt gctcttactt aaatggatca
     2041 atagcaattt catcacaaac ctatagagga tgagtgactt ctgcctaatt acgttaaaaa
     2101 atattgaaag taactctatg tagcaaaaaa tatctagaat ttcatttcct caaaactgat
     2161 cgatattgta tctaaaaata tatactacct aaaataggta gtatatatta aaatatgtgc
     2221 ttttgttaga aaaatcaata cataaattat catatctgta tatgtcattt aggtatttca
     2281 gttatgttat aatagttttg ctgtctgcta ccccggccta atctggcaag gggaggagga
     2341 gatataatga aatacctttt tgattttgtt tttgcaatca agccatttgc agaggtaact
     2401 gcacactaca tctgcaagtg gcttgattgc aaaattgcca aaaaagacag taagcctacc
     2461 agttaattct ataatataga aacgtaaaaa aaaccccatc agcctacacg ctgatggggt
     2521 ttgatgatat aatgaaatac ctctttgtta ctcataatta taccataatt agtcacgaat
     2581 gcaaccaata gaaaaaacta cttagaacgg tagatcatcg ctaataacaa tgcattgtcc
     2641 accaaatgga ttttgtgaag cattaaatac attgttatta attgtcgctg cttttaacta
     2701 ggcctctccc cttctattat aaaagaagat atatattatt tcttaattac ctactacctt
     2761 a
//
LOCUS       DAPBBG010000135         2490 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPBBG010000135 DAPBBG010000000
VERSION     DAPBBG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.143"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(365..1657)
                     /locus_tag="RD477_002859"
     CDS             complement(365..1657)
                     /locus_tag="RD477_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7635145.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="RD477_002860"
     CDS             complement(1931..2191)
                     /locus_tag="RD477_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635146.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          381 g          800 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaaggcgct tctgtagaat
     2401 aaagttaacg acaaccaatt cacagaaaag aggacttccc tatgaatgat tttactacag
     2461 aaattgtgca aactctagtc actaaaggcg
//
LOCUS       DAPBBG010000136         2419 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPBBG010000136 DAPBBG010000000
VERSION     DAPBBG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.144"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..287
                     /locus_tag="RD477_002861"
     CDS             <1..287
                     /locus_tag="RD477_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635147.1"
                     /translation="TQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNKN
                     YFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(800..873)
                     /locus_tag="RD477_002862"
     tRNA            complement(800..873)
                     /locus_tag="RD477_002862"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Ile,seq:cat)
     gene            complement(1062..2081)
                     /locus_tag="RD477_002863"
     CDS             complement(1062..2081)
                     /locus_tag="RD477_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDT7635148.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2092..2298)
                     /locus_tag="RD477_002864"
     CDS             complement(2092..2298)
                     /locus_tag="RD477_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDT7635149.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          739 a          466 c          404 g          810 t
ORIGIN      
        1 agacacaatt atggtttaaa tttgaccctt cgaatagatt tatcaaagat ttttataaag
       61 tatggaattc agaagttttc tttttagcaa tcgaagatag cttattaatc aatctctact
      121 attctaataa gaactatttc aaaattcctg ctgcgaaaac tagaatgaag aaggatgtat
      181 actttttgtt tgatatcgtg actgacgtgc cggacgcacg tagcgatcat cggcgttatg
      241 actatataaa gtatactttc gttgatccag aaagatacaa agattaaagt aggctaccta
      301 aaaaggtagc ccggaacgaa ttttatcacc atacttgtga aaggagatat tttttaagtt
      361 agtattaaga ttgtgtaata tgatgatatc tatattttat agtatcagtg ctataaaatc
      421 aaaaataagt cactaattaa ctaccactcc aattgtaagc cttttttctc actttttttc
      481 aaaaatatgg tatgcctttt aatagcttca aatataaaag agtttaaagc gtaacacact
      541 tatggggaag tggcttgggg tgcgctttaa actcttcttt attatcatct cacaatttaa
      601 cccaaatgtc tttctattta aaaatcaaag taaaactttt caaatataca gaaatataac
      661 tatgtgaaac atcctttcat taatccataa aaggatacat aaaaaagcca ctcatttgag
      721 tggcaatgaa gaaaagcttt agcttgtata atactcttca aaaaattcta acacagaacg
      781 attcaaatgg ctacgttaat gtaccctgta ggactcgaac ctacgaccgg acggttatga
      841 gccgtctgct ctgaccaact gagctaaggg tactggttgt tgccacataa agccataaac
      901 aatcaaccag tagaatgtgt ggcaacaaac ctgttatcgc atagcttgga gtgtgactat
      961 tatgggtgat agtgaagata tgcgataaca tcactatttt atcgaatgat ttttatagtt
     1021 gtcaatatag ttatgtaatg ctcctcaacg aggagctatt tttatcgttt aggaatattt
     1081 aaataccaac gtttgtcatg gaaatcttgc gcaccgcctt tagtgtttcc ctctggatca
     1141 ttcgttgccc gcatcatgac atagactttc ttattaggga agttacgcat gttaaaagat
     1201 acatgatagc caacatttcc agaagtatta taagcttgat ttacatctga tctataaatt
     1261 ccatcagctc ttactcgagc taattctttc ccagtattgt aatccataat gaagatatac
     1321 tcgtatttat agttagcaat gtgccatcca gctacatgca aatttgcatt ttcgatttct
     1381 ccaaactgat caatgtgggc gtaattcgtt ccatctgtca gtgtaggatt agctgcacca
     1441 gctcgtgttg gatcaatgac aggcttgttt tcagaagttg ttggattttc atcggtaaat
     1501 ccatgagcta aatcatatgc taatttttct ttacttacgc ccatttcaga aagataaccg
     1561 taaggatctg tatgatcgcc ccagatgttt tgtgttaccc ataaatgcga tttgattccc
     1621 ggttggttat aaggagtgtc caacgttaat ggaataccat atttcattgc tgaatctcta
     1681 gccaattcaa cgtatgcctt gtagtttttc tcaaacgttg ctttatcatg tgtgtgttgt
     1741 aactcaatct gcacaggact gttggcatta gcatacgaac cagcaccgta ctgtacataa
     1801 ccaggttgac cgacttgata aacaattccg ccgtctccca caatataagc agtataagcg
     1861 ctagtccatg aacgttgcat atactgcgct tcattgcgtc ctgttgctgt ttcattagcc
     1921 gtttcatgca gtaaaatata ctgattattt gctacttgtg agctaccttc atttgcgccc
     1981 aaattaaatt cattgttgat agtataggca aatccgttaa ttggcaataa aaaaagagcc
     2041 gttaacaggc tcatcgcagt aatagtaatt ttctttttca tatctatcct cctatttatc
     2101 tgatgaaatt ttcttatacg ttcgatttga tacacctaaa atactcccta gaaatgctcc
     2161 aaaacctgta ataatcatca ccgttatatc tgtgtaccgc cagttaacac cttttccgac
     2221 aagaccgaca aaagtcgcta aagcggggat aactactata gcgatccatt tcaaaatatc
     2281 aaaggttcta ttgttcatac tatccacctc ccttcattgg ttgggctact ttctttttca
     2341 atcgcttgtt ttcttctatc aatatttctc tttctgaaag caaggcatca atttgcccgt
     2401 ccagttgttc aattttatc
//
LOCUS       DAPBBG010000137         2401 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPBBG010000137 DAPBBG010000000
VERSION     DAPBBG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.145"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            207..2069
                     /locus_tag="RD477_002865"
     CDS             207..2069
                     /locus_tag="RD477_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7635150.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="RD477_002866"
     CDS             2069..2401
                     /locus_tag="RD477_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7635151.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 a
//
LOCUS       DAPBBG010000138         2307 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPBBG010000138 DAPBBG010000000
VERSION     DAPBBG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.146"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(70..141)
                     /locus_tag="RD477_002867"
     tRNA            complement(70..141)
                     /locus_tag="RD477_002867"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Glu,seq:ttc)
     gene            complement(153..226)
                     /locus_tag="RD477_002868"
     tRNA            complement(153..226)
                     /locus_tag="RD477_002868"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ile,seq:gat)
     gene            complement(239..309)
                     /locus_tag="RD477_002869"
     tRNA            complement(239..309)
                     /locus_tag="RD477_002869"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(275..277),aa:Gly,seq:tcc)
     gene            complement(314..386)
                     /locus_tag="RD477_002870"
     tRNA            complement(314..386)
                     /locus_tag="RD477_002870"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Phe,seq:gaa)
     gene            complement(390..462)
                     /locus_tag="RD477_002871"
     tRNA            complement(390..462)
                     /locus_tag="RD477_002871"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Asp,seq:gtc)
     gene            complement(481..554)
                     /locus_tag="RD477_002872"
     tRNA            complement(481..554)
                     /locus_tag="RD477_002872"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Met,seq:cat)
     gene            complement(567..656)
                     /locus_tag="RD477_002873"
     tRNA            complement(567..656)
                     /locus_tag="RD477_002873"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(618..620),aa:Ser,seq:tga)
     gene            complement(679..750)
                     /locus_tag="RD477_002874"
     tRNA            complement(679..750)
                     /locus_tag="RD477_002874"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(715..717),aa:Glu,seq:ttc)
     gene            complement(772..859)
                     /locus_tag="RD477_002875"
     tRNA            complement(772..859)
                     /locus_tag="RD477_002875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Ser,seq:gct)
     gene            complement(884..957)
                     /locus_tag="RD477_002876"
     tRNA            complement(884..957)
                     /locus_tag="RD477_002876"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Ile,seq:gat)
     gene            complement(971..1041)
                     /locus_tag="RD477_002877"
     tRNA            complement(971..1041)
                     /locus_tag="RD477_002877"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1007..1009),aa:Gly,seq:tcc)
     gene            complement(1043..1118)
                     /locus_tag="RD477_002878"
     tRNA            complement(1043..1118)
                     /locus_tag="RD477_002878"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Asp,seq:gtc)
     gene            complement(1137..1210)
                     /locus_tag="RD477_002879"
     tRNA            complement(1137..1210)
                     /locus_tag="RD477_002879"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1174..1176),aa:Met,seq:cat)
     gene            complement(1223..1312)
                     /locus_tag="RD477_002880"
     tRNA            complement(1223..1312)
                     /locus_tag="RD477_002880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1274..1276),aa:Ser,seq:tga)
     gene            complement(1336..1409)
                     /locus_tag="RD477_002881"
     tRNA            complement(1336..1409)
                     /locus_tag="RD477_002881"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1373..1375),aa:Ile,seq:cat)
     gene            complement(1423..1496)
                     /locus_tag="RD477_002882"
     tRNA            complement(1423..1496)
                     /locus_tag="RD477_002882"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1460..1462),aa:Met,seq:cat)
     gene            complement(1524..1596)
                     /locus_tag="RD477_002883"
     tRNA            complement(1524..1596)
                     /locus_tag="RD477_002883"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1561..1563),aa:Ala,seq:tgc)
     gene            complement(1616..1689)
                     /locus_tag="RD477_002884"
     tRNA            complement(1616..1689)
                     /locus_tag="RD477_002884"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Pro,seq:tgg)
     gene            complement(1699..1772)
                     /locus_tag="RD477_002885"
     tRNA            complement(1699..1772)
                     /locus_tag="RD477_002885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1736..1738),aa:Arg,seq:acg)
     gene            complement(1783..1868)
                     /locus_tag="RD477_002886"
     tRNA            complement(1783..1868)
                     /locus_tag="RD477_002886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1832..1834),aa:Leu,seq:taa)
     gene            complement(1885..1956)
                     /locus_tag="RD477_002887"
     tRNA            complement(1885..1956)
                     /locus_tag="RD477_002887"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1922..1924),aa:Gly,seq:gcc)
     gene            complement(1968..2040)
                     /locus_tag="RD477_002888"
     tRNA            complement(1968..2040)
                     /locus_tag="RD477_002888"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2005..2007),aa:Thr,seq:tgt)
     gene            complement(2058..2139)
                     /locus_tag="RD477_002889"
     tRNA            complement(2058..2139)
                     /locus_tag="RD477_002889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2103..2105),aa:Leu,seq:tag)
     gene            complement(2147..2219)
                     /locus_tag="RD477_002890"
     tRNA            complement(2147..2219)
                     /locus_tag="RD477_002890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2184..2186),aa:Lys,seq:ttt)
     gene            complement(2223..2295)
                     /locus_tag="RD477_002891"
     tRNA            complement(2223..2295)
                     /locus_tag="RD477_002891"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2260..2262),aa:Val,seq:tac)
BASE COUNT          627 a          636 c          541 g          503 t
ORIGIN      
        1 aactctgcta agctttttaa aatcattctt tcagataaaa agaaaaagat actcaaaaag
       61 tatcttccat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct
      121 taaccgcttg accaacgggc caataaaata aatgggccta aatggactcg aaccatcgac
      181 ctcacgctta tcaggcgtgc gctctaacca gctgagctat aggcccataa aaactaaaag
      241 cgggtgacga gaatcgaact cgcgacaaca gcttggaagg ctgtggtttt accactaaac
      301 tacacccgca taatggcgcg ggacagaatc gaactgccga cacatggagc ttcaatccat
      361 tgctctacca actgagctac cgagccaaac ggttccgacg ggaatcgaac ccgcgatctc
      421 ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa ccaatgtttt tagtttttaa
      481 ttgcgggagc aggatttgaa cctacgacct tcgggttatg agcccgacga gctaccagac
      541 tgctccatcc cgcgataata tgaaaaagga ggataaggga ttcgaaccct tgcacggttt
      601 tacccgcctg acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccatgt
      661 aaaactatat taaattaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg
      721 gcggtgtctt aaccgcttga ccaacgggcc aggtaaaaca aaatattgaa acggagaagg
      781 agggatttga accctcgcgc cggtttcccg acctacaccc ttagcagggg cgcctcttca
      841 gccacttgag tacttcccca aaaccaataa gattttgtac taatgggcct aaatggactc
      901 gaaccatcga cctcacgctt atcaggcgtg cgctctaacc agctgagcta taggcccata
      961 aaaaactaaa agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt
     1021 ttaccactaa actacacccg catggcggtt ccgacgggaa tcgaacccgc gatctcctgc
     1081 gtgacaggca ggcatgttaa cccctacacc acggaaccag tgtttttagt ttttaattgc
     1141 gggagcagga tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccagactgct
     1201 ccatcccgcg ataatatgaa aaaggaggat aagggattcg aacccttgca cggttttacc
     1261 cgcctgacgg ttttcaagac cgttcccttc agccggactt gggtaatcct ccatatgaaa
     1321 cttacaacgc cacaatggac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
     1381 tctgctctaa ccaactgagc taaaggtcca ggctcttaaa aatcgcggcg gaggggatcg
     1441 aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac accgcgaaaa
     1501 agatattcat tatttaaatg aaatggagcc tagcgggatc gaaccgctga cctcctgcgt
     1561 gcaaagcagg cgctctccca gctgagctaa ggccccgtgt ttaaatttat aattatcggg
     1621 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     1681 tacttcccgt ttaattaacg cgcccaagag gagtcgaacc cctaaccttt tgatccgtag
     1741 tcaaacactc tatccagttg agctatgggc gcatatttga aatgccgagg accggaatcg
     1801 aaccggtacg gtgatcactc accgcaggat tttaagtcct gtgcgtctgc cagttccgcc
     1861 accccggcgc gattgctttg gcaaagcgga aaacggggtt cgaacccgcg acccccacct
     1921 tggcaaggtg gtgctctacc actgagctat ttccgcgtgt ttaaagatgc cggctaaagg
     1981 acttgaaccc tcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc
     2041 atgtaaaact taaattatgc gggtgaaggg acttgaaccc ccacgccgta aggcgctaga
     2101 tcctaaatct agtgcgtctg ccaattccgc cacacccgca aaaatatgag ccgtacaggg
     2161 ctcgaacctg tgaccctctg attaaaagtc agatgctcta ccaactgagc taacggctca
     2221 tatggaggtt aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag
     2281 ctgagctaaa cctccatgga taactta
//
LOCUS       DAPBBG010000139         2157 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPBBG010000139 DAPBBG010000000
VERSION     DAPBBG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.149"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..147
                     /locus_tag="RD477_002892"
     CDS             <1..147
                     /locus_tag="RD477_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7635152.1"
                     /translation="EILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILSA
                     IRVF"
     gene            complement(434..919)
                     /gene="vanZ"
                     /locus_tag="RD477_002893"
     CDS             complement(434..919)
                     /gene="vanZ"
                     /locus_tag="RD477_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDT7635153.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1072..1983)
                     /locus_tag="RD477_002894"
     CDS             complement(1072..1983)
                     /locus_tag="RD477_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDT7635154.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          682 a          411 c          306 g          758 t
ORIGIN      
        1 gaaatactta ctgctagtgt cgctacaacg tcacaccatt tacgtttttt aaaaaaacat
       61 ggaatggcaa aatcacgtca agatggaaaa gtagtttatt actctttagc gaatgaagac
      121 atcctttccg ctattcgtgt tttttaaagt tatcagaaaa tctatctatg aaatcttagt
      181 ttgggggtac cgtcaggaaa atgcggattt acaacgttaa ggaaatttat taaagagacc
      241 aattcctcag cactaaaatt gataattggg ctttctttgt tactatagtg ttaggtaagc
      301 tttttacttt atcaccggtt ttcccctcac ttcacaccta cgggcgagtt tccccacatc
      361 ccgttttcca tctaatccaa ttcattcaat atctttatgt gtatgaaaga tcagattgct
      421 tgatttagac atcttacacg taatttattc ttaaatgggt acggtaaacg agcaataata
      481 cgagcaaaag tatacctaca aaaataataa ctctgtctaa ttttttttga ttcatatgct
      541 tattgcttaa accatataat ttcagtccaa gaaagcctcc aacagtattt gtaattacat
      601 ctgttatgtc tgtcgctcca atagcgaaga taaattgaat tatttcaaaa gtaagactta
      661 aaaccagtac aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg acattcaaaa
      721 gcaagccaaa tggaataaag attataacat tatctatcat ctctctgaaa ttcccagtag
      781 cagtaaatgg agtcaagtta agacttcttt gatgataatt aaatactgat aaaatattgt
      841 attgtaattt gaataacact aaccagatta gtgtcactaa atataaagct agcaatcctc
      901 tagataatat ttttcccaat ataatttcct ctaattcttt tgtttaagtt gtatcattag
      961 tatagcaatg tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt
     1021 gctagtacaa taatgctgtt cattaatttc gttggtttca ttccgccatc cttacctcct
     1081 tgaattagta tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta tattgtttcc
     1141 tgatatctca taacgaagat tagtcggcac atgaatggtg gtatttttag taacaggata
     1201 ataaaagatc tcatattttt ccccatttac actaacagaa atggtttttt cttcttttag
     1261 gtaatccata tattcctcga gaacgaaatt cttttctttc ataatcgcac tatgtggtaa
     1321 accaacatag cgaatatgcc atggttcata ttgaattcct gttaactctg ttttgtcctc
     1381 tggataacgt aaaatgaacc cgtatttcca agcattttct tctatccact ttccttcagg
     1441 ggctcgttcc attttcgtca agcttgatcc tacatctagt gataaacctg aattatgctc
     1501 actataacct gctggtaagg catactcagc ccccatttct tggtaaagca cactttgctc
     1561 atcaaagtct cgatagccac tattaataat aaaatgacta acgccaccct ttacagcatc
     1621 attgaccatc tctgaaaatt tttgtgctat ttcttttgac atataaatat tactatcaag
     1681 caacccgtat ccatttatta attcgtcatg tttagataaa ttcacgatat ctgacttcac
     1741 actttcttgg cgaacaggat atttactatt gattaatagc agatttcctt gataaacctg
     1801 ttcttctgta attgttttct cttgggtatt ttcagaagtc ccactatttt ctaaatgttc
     1861 tttgggattt tgatcataat tttgaaattc gactttttcc tgagaaaaca gtgcttcatt
     1921 aacgtagtca taacctaagt atattaagaa taacaataac aataaaaaaa acaacttctt
     1981 cattttcagt ctcctcctta actatatagt tggaagtatg aaatatttct agaatcttcc
     2041 ccttaaacgg cacaatctcg tttgagattg tgccgttttg ccccgtaaat tacagtcaat
     2101 tatttcgccc tactggattt actattatca ccaatgtaga tgaattttca taagtga
//
LOCUS       DAPBBG010000140         2069 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPBBG010000140 DAPBBG010000000
VERSION     DAPBBG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.150"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(205..735)
                     /locus_tag="RD477_002895"
                     /pseudo
     CDS             complement(205..735)
                     /locus_tag="RD477_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            807..>2069
                     /locus_tag="RD477_002896"
     CDS             807..>2069
                     /locus_tag="RD477_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7635155.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMI"
BASE COUNT          659 a          399 c          368 g          643 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taacatttcc atttcattta
      121 cctctttttc gtttaattat actaaagttc tatccaagcg acaagctgtg tataataaaa
      181 atatacccgt ggtgctagtt aattttaata ccctaaagaa tatctcaaag tcccttcaaa
      241 atgttgctgt gctcggctta aaagaatact gtacgataat aatatgcttt caaacttact
      301 ttctagccct tgaatagaac gtgatttgaa attttgaaaa cctagtcttt cgagagaaga
      361 aaaaacagtt tctactgctt ttctttgtct agtaagtaat cgatggtcta tcttccctag
      421 tttatccatg ttttttcgtg acggaactaa gactgtaatg cccttaaggg caagcttttc
      481 atggatttta tggctgacat agcctttatc tccaaggata tttggaaggt ttgcttcttc
      541 cgataaagtc tctaaaactt tcacatcatg aacactagga tccgtgattg aataagtaac
      601 tggaaagcct gttttagtga cgataatatg aattttaaga ccataaaaat aagcgttttt
      661 cgttgagtta tagcccctac agctctatac tttttgatgt tggtggatga caccagcatc
      721 ttttttattt tccaaaacat atagaaaaga caccttgaaa tcctttagaa ttaatttggc
      781 gaaccaaccc aaaaggagga atcaagatgt cttacactca ccttatcaaa gaaacattag
      841 atattctaga cttatcagtt acttttaacg aaaattgtct cacaaaagaa aaatacaaag
      901 ggcaaatctg tcatatttat cgtggaaact tgatttatac agcccaagaa tgcatccact
      961 gtaaacacca gattgcttca gatattgtgc gttggggtac aacaaccgtc cggttgttaa
     1021 tgaacgatgt ttctgaatgc cgtacctacc tcgaattgaa aaagcaacgc ttcaaatgca
     1081 aagcttgtca gcggaccttt gtcgcagata cttctgttgc gaaaaaacac tgtttcatca
     1141 gtgagaaagt ccgctggtct gtcgtgactc gtctgaaaaa aaatacctca atgacagaga
     1201 tcgccgctca gaaaaatcta tcggtctctt ctgtttaccg tattatgaag cgattttatc
     1261 gtccgttgaa tccttttcgg acaccgttac ctaaggtact ttgttttgat gaattcaaat
     1321 cagttcgggg tgtttcaggt gctatgagtt tcatcatgat ggatggacaa acacagcgct
     1381 tgttggacat tgtcgagaac cgacaactgc cttttttaaa acgttatttt agtcattttt
     1441 cacgagaaat tcgtgaagcg gtcgaatgga tcgtgattga tatgtacgct ccttatgtat
     1501 cgctcgttaa aaagctgttt ccaaaagcac agctgattat tgaccggttt cacatcgtcc
     1561 aacatattgg tcgtaccttt cgcaatcatc gaatcaaaga aacgaaccaa ctattaaaaa
     1621 gcaaagaaca aaaacactat caattaggta agcaattaaa aagatactgg aaacttcttc
     1681 aaaaagatga acgcaagctt gattatactc gacgcctttg gcgacctggc ttcaaagctc
     1741 atttaacaga gaccgatatt gtggatcgct tactgaaagg gagccctgct ttgcgtgtag
     1801 gctatcagtt atatcaagac tttctttacg ctgtaaagga acgagactac gtatcgtttg
     1861 aagaactact taccaataat atcatgcttc ccgaaggcta tcagacgacc ttgagaacgt
     1921 tccagaaatt tctgccacaa atcaaaaatg ctttacaaca aagttattca aatggcccac
     1981 ttgaatgtct gaacaaccat atcaaagtgt taaaacgaaa tgcatatggc tttcgtagct
     2041 tttataattt caaattgcgc atcatgatt
//
LOCUS       DAPBBG010000141         2001 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPBBG010000141 DAPBBG010000000
VERSION     DAPBBG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.151"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..376
                     /locus_tag="RD477_002897"
     CDS             <1..376
                     /locus_tag="RD477_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDT7635156.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="RD477_002898"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="RD477_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDT7635157.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="RD477_002899"
     CDS             1857..>2001
                     /locus_tag="RD477_002899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635158.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DAPBBG010000142         1976 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPBBG010000142 DAPBBG010000000
VERSION     DAPBBG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.152"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            128..1852
                     /locus_tag="RD477_002900"
     CDS             128..1852
                     /locus_tag="RD477_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7635159.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          773 a          240 c          347 g          616 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaaatta
       61 tatcttctta aacaatgata atattaaaat gcaagcgctt gtaaaaaata aggagaagag
      121 gtaaaaaatg aagaaaaaac tattaacttt atttcttgtg atgtctttct caatcgtttt
      181 gagtgtgtac tacaatacta ttgttttttc agaacaaata aactacacag aagaacagta
      241 taatatagaa aaagaaaaag ctttaaaagt tggttatagt gaagaacagt ttaagcagat
      301 tatggaaata cctacaaatt tatcaaatga aaatagtgaa acaagaatag taaattatac
      361 tatgacttct aatcaaacca aagtcattaa taaagcaatg gagcaaattg gaaagccata
      421 cgaatgggga gcatcgggac caaattcttt tgactgtggt ggtttagtga aatatgttta
      481 caaacaagct gtaaatattg aactgcctat gggaaccacc aatcaagagc aatacggtac
      541 agaggtaagt ttaaattcac ttaagcctgg tgatttatta ttttacggca atcgaggtgc
      601 aacttatcat gtaggtatat ataaaggtaa cggtgtaatg attcatgcac cgcaacctgg
      661 agaaactgta aaagaggtta acatacaata tttttatcct aactttgcaa aaagaatttt
      721 gcctgatgaa cctgactacc cttatattga ctataataaa atggtaactg ttacaaaagc
      781 ttggtcaata tggaatgatt tacaatttag tcatgaaatt aagaaagcta ttattggaga
      841 taattacaag atagggaagg tttatactaa tccagaaaat aataataaat atggagaaat
      901 attagtaagt aataaagtat atggatacat taatttttat gctgttaaag agttgacctc
      961 tgttcaaata aacagatatt taacttctaa agatagtggt caaccaactt ggggaaatct
     1021 agagtgtact ataagtaaag gacaaactac aaaagataaa atatattttg ttaaaggagc
     1081 atataattta ggggatggaa agtatctata ttctatttat aaagatcagg attcctctga
     1141 atggttaggg tatttaaaag cacatgttag tttagcctat actcctattg aagaaattaa
     1201 taaaaacgta actgttacta aaaattggtc gatttggaat aatctacaat gggaaaagga
     1261 aattgaaaag cctcagattg gttctgtatt ttcagctaga ttaaagttaa ccaatgtatc
     1321 gaataatgct gtctattaca agctttataa atctggaaaa ttttatggtt atatcaatgc
     1381 tgaggctgtc aaagacctta caacaacaaa gcttaataag tatgttacat ttagtgttaa
     1441 taatgaagat ttttggtctt ctttagatgt aaattattca aaaggaaaaa ctgaaagagg
     1501 ccgagtattt tacattagta tttcatataa taccgcagat aaccaaccga tttattcagt
     1561 atatacagat gaaacatgta cggaatggcg agggtattat aagggaaaca actttgaaga
     1621 cactcaaatt actatgcttg agaataaaag cgtgaaagta acaaaaagtg ggtatacagt
     1681 atggggagac ttgaattttt ggactaagtc aggaatatcg aacacaggag atatatacac
     1741 cactgaccga aaattttata attttaccaa caatgcttac tattatgaat tgaaaaaaga
     1801 tgggagggtc tatggctata taaatagtga agcagctgta gagatgaact aaaatattgc
     1861 tgtcccttaa tcttatgggg cagcaatatt tttatattat taatcatttt tctgaagcga
     1921 gggtagaaga attaatcaat agtttttctt gttttgtaat ataatagttt aattac
//
LOCUS       DAPBBG010000143         1811 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPBBG010000143 DAPBBG010000000
VERSION     DAPBBG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.153"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..712
                     /locus_tag="RD477_002901"
     CDS             <1..712
                     /locus_tag="RD477_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7635160.1"
                     /translation="PPNSLSTEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGG
                     FLGYVANRGNKYTFELAQSFSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVT
                     QYLVTTEFDDDTVQAIMDEALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPR
                     TAQQQYDVTQHIPLSEAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYAD
                     LTSPYWQQHLVGAGRIKQ"
     gene            709..1641
                     /locus_tag="RD477_002902"
     CDS             709..1641
                     /locus_tag="RD477_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7635161.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
BASE COUNT          605 a          328 c          375 g          503 t
ORIGIN      
        1 tccacctaat tcattgagta cagaagaatc cattaagcaa ggtgtgaagt atttcagtga
       61 attattagcc agtagcgaaa ggctcagtgt agatttagaa tcggttatcc agtcctacaa
      121 ttatggtggt ggtttcttag ggtatgtggc taatcgtgga aataaatata cctttgaact
      181 ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa gtgtcttacc ccaatcccat
      241 agccatacct atcaatgggg gctggcgata caactatggc aatatgtttt atgtgcaact
      301 ggtaacgcag tatcttgtca caacagagtt tgatgatgat acggtacaag ccatcatgga
      361 cgaagcactg aaatatgagg gctggcgata cgtttacggt ggagcttccc cgactacttc
      421 ttttgattgt agcggactga cacaatggac gtatggaaaa gctggaatta acttaccacg
      481 aaccgcacaa cagcaatatg atgtgaccca gcatatccca ctatcggaag cacaagctgg
      541 cgatttggtt ttctttcatt ctacctataa cgctggctct tatattactc atgttgggat
      601 ataccttggc aataaccgta tgtttcatgc aggcgaccca atcggttatg ccgacttaac
      661 aagcccctac tggcaacagc atttagtggg agcaggacga atcaaacaat gagaaaggaa
      721 gatttaatga tgaaatttag aaaaaatcag aataaagaaa aacagatacc aaaggaaaag
      781 aaacctcgtg tctataaggt caatcctcat aaaaaggttg tgattgcctt gtgggtactt
      841 ttagggctta gtttcagctt tgcgatattc aagcacttta cagctataga tactcatact
      901 attcacgaaa caactatcat agaaaaggaa tacgttgata ctcatcatgt agaaaatttt
      961 gtagagaact ttgcgaaagt ctactattca tgggagcaat ccgataagtc cattgataat
     1021 cgaatggaaa gtctaaaagg ctatctgaca gatgaacttc aagctctcaa tgttgataca
     1081 gtacgcaaag atattcctgt atcgtcttct gtaagaggat ttcagatatg gacggtagag
     1141 ccaactggcg acaatgagtt taatgtaacc tacagtgtag accagctcat tacagaggga
     1201 gaaaatacaa agaccgtcca ctctgcttat atagtgagtg tctatgtaga tggttctgga
     1261 aatatggtac tggttaagaa tccgaccatt accaacatac ctaagaaatc aagttataaa
     1321 ccaaaagcca ttgaaagtga ggggacggtt gattccatta caaccaatga aatcaatgag
     1381 tttttaacga cgttcttcaa gctctatcct acagcgacag ccagtgaact ttcctactat
     1441 gtgaatgacg ggatattaaa accaatcgga aaagagtaca tctttcaaga actggtaaat
     1501 cctattcaca atcgtaagga taatcaagtc acggtatcgc tgacagtgga gtatatcgac
     1561 cagcagacca aagcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt
     1621 aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg
     1681 tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt
     1741 taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
     1801 aatttgatag c
//
LOCUS       DAPBBG010000144         1629 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPBBG010000144 DAPBBG010000000
VERSION     DAPBBG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.154"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            287..694
                     /locus_tag="RD477_002903"
     CDS             287..694
                     /locus_tag="RD477_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635162.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="RD477_002904"
     CDS             708..1109
                     /locus_tag="RD477_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635163.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="RD477_002905"
     CDS             1111..1482
                     /locus_tag="RD477_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635164.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          612 a          209 c          283 g          525 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtataca
//
LOCUS       DAPBBG010000145         1596 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPBBG010000145 DAPBBG010000000
VERSION     DAPBBG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.155"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            481..1035
                     /locus_tag="RD477_002906"
     CDS             481..1035
                     /locus_tag="RD477_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7635165.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1289..>1596
                     /locus_tag="RD477_002907"
     CDS             1289..>1596
                     /locus_tag="RD477_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635166.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          642 a          166 c          268 g          520 t
ORIGIN      
        1 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
       61 acaaaattat gatcttgcgt aaagttataa aatatcaata aaaatatttt attaatgaaa
      121 agcttggtag cacggttatt catatgctaa tttatttcac tttatagtaa ggggagttct
      181 tagtatattg ttcaaactta aatttcatat aatttaatta tgtaaattag ctattctaag
      241 aacttctacg gataattgcc cgtctaaaat ttaaataaga ggttagaatc ttcatataag
      301 taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa
      361 atcataaact aaaccccttg attttcttca cttttaacag agtatatata taagttcatg
      421 attatggtaa aatatatata taatcatgaa ctcagaatat ttttaagaag ggaaacaata
      481 gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg
      541 ctaagaagaa caagtaatcc agaaagagat agttttttat ttttgtttgg aatcaataat
      601 gggttacgta tgtcagatat tataggatta aaagttggag acatacgagg taaaagtaag
      661 cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga
      721 gaagagattt tattatatac tgaaggtaaa gaagaaaatg attggttatt tccaagtcgg
      781 caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa
      841 aaattaggaa gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac
      901 tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca
      961 attacaaaaa ggtacattgg tataactgaa gatgaaatag gggcatcgtt acgtggtttt
     1021 aaattgggag gctaattgag atattaggat atattttttg tgtataatga aaacttagaa
     1081 ggtgagatta atgaagataa ctgaattttc ggaagtattt aaaacgttta gtaacattta
     1141 caatagaata ccagagatag aacaaaatgt tgtagttgat aatgattatt atatagagaa
     1201 tgattttaat aaaccaagaa aatatgacaa cctatacaat tttatacaca atagaacaac
     1261 aataaataac aacagtaaca ctcgagtttt gattaattca gcatattttc atattaaata
     1321 tttaaagaaa tatgagtatg aagaagttgt attagattat gggctaaata gacagtcatt
     1381 tttaatttat gcaataaata acggaaattt gccaagcaat aaaaagaaca taattattga
     1441 aacatatgcc tatctggagg ggggatctaa aaaactaatt aagtcatgta atatagagta
     1501 tcaattatca gtaggagaaa ttaaatgtgt tttcgaagaa gatttagaac ttgacttaaa
     1561 aaaaattttt gccagcattc atgattatat aggttt
//
LOCUS       DAPBBG010000146         1525 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPBBG010000146 DAPBBG010000000
VERSION     DAPBBG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.156"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            263..997
                     /locus_tag="RD477_002908"
     CDS             263..997
                     /locus_tag="RD477_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635167.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKGKRIMVPLQSKKTSSASKVRTFF"
     gene            complement(994..1491)
                     /locus_tag="RD477_002909"
     CDS             complement(994..1491)
                     /locus_tag="RD477_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7635168.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRIYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          495 a          261 c          243 g          526 t
ORIGIN      
        1 gtttgctaga tccaaactct tattaaactt tccttgctat aaaacccaaa catagtaata
       61 aaagaataac ttattacctt taaattatac taccaacaaa tggtagtata ttattaattt
      121 atataatatg aaacctaaaa tatagtagta tacccgtggt gctagttaat tttttcctag
      181 aggaaaatgt ctaaacatgt tggtaaatca acaaaaaaac gcatatagta gtctcaaatc
      241 tcccaagaaa tgaggactac atatgcgttc atcaaaatat actgaacatt ttgacttagc
      301 caatcctgtt actaaagtcg atgatatccc cgattatgaa atgtacagtc aaacaatcga
      361 tagcttaaat aagcgttttg gcaatcgtgt tttaaaaggc atcgaaatcg gttacatcgc
      421 ttctgaaaag gatcggatca tcgactattt agccgataaa gattacgatt taaaattact
      481 cagtgtgcat cataatggtc aatttgatta tttagatgac gaagtcaaag atatggatcc
      541 tgctattgtg attcctcagt attttgcaca actttcagaa gcactagttg ttatagaagc
      601 agatgtcttt gctcattttg actatggttt tcgggtattt ggtctatccg ttgctgaatt
      661 taaacaatat gaagcgcagt ttttaccgat tctagatcaa gtaatcaaaa ataaattggc
      721 attcgaatta aatgctaaga gcgcttactt gtatgacaat ttagcgttat atgaatatgt
      781 catcgattta tatctttcac gtggtggtac cttgtttagt gttggatcag atgggcatta
      841 ccttgaacat ttccgtttcc atttcgatga tctttttgct ctgttaaaag caaaaggtgt
      901 gacggaatta gcgatttatc aaaaaggaaa gcgaattatg gttccattac agtctaaaaa
      961 aacgtcctca gcatcaaaag tgaggacgtt tttttagatt tctttttcta aaatcggaca
     1021 aatacgatct tgttttccta aagggaaatg tttgatcccg atttttttaa atccatgacg
     1081 ttcataaaaa cgaatacctg catgattttt ttcataaaca gtcaaacgta aataacgttt
     1141 ttgtttcgtt ttagcataac gattcgcttc atcgagtaac cgttgcccta accctttgtt
     1201 ttgataatct ttgagcagat agatccgttg aatctctata gcatcttttt catcattgac
     1261 tttcatataa ccaatgagta tttgatgatc cttaatacca ataaatttac tggttgactg
     1321 tgccaattct actgccaata tttcagttgt caattgtgtc tgtaaatatc cttgcatatc
     1381 aagtggatct gcgccatcga taaacgattc ataaaaactg acaacactta caacttgtaa
     1441 agcatctaga tcatcataat ctaaatacgc tagtgtgatt tgattgttca tataactcct
     1501 ccttttgaca tagtatacta taaat
//
LOCUS       DAPBBG010000147         1519 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPBBG010000147 DAPBBG010000000
VERSION     DAPBBG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.157"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..418)
                     /gene="tnpA"
                     /locus_tag="RD477_002910"
     CDS             complement(<1..418)
                     /gene="tnpA"
                     /locus_tag="RD477_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDT7635169.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYI"
     gene            661..1519
                     /locus_tag="RD477_002911"
                     /pseudo
     CDS             661..1519
                     /locus_tag="RD477_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          502 a          286 c          295 g          436 t
ORIGIN      
        1 taatatatcg aatgcgatca tcagcttctt gttcacggat gtatttttca tttgtctttt
       61 gattcaatcc gactgtactc acatagtatc tcttcgacca aaaggatttg tttccgtatt
      121 tatatttgag atttgcgtgc tgctcgtgga ttagtaccgc actacgacct ttcagatacc
      181 ccataaaact ggatatacat atttttggtg gtactcgaac aagcatgtga atatgatcta
      241 gcattgcatg tgcttctatg atttccacat ctttcatttc gcacaacttc ctaagtattt
      301 ttccaagatc ttttcgtaat tgcccataga ttacttttcg ttgatatttt ggtgtaaaaa
      361 taatatgata cttacaattc caacgagtat atgctaaact tttatcgtca tttgacatga
      421 aaaaactcct ttcgatctga tgagcggttg gtagccactc actagtatat cgaaaggagt
      481 ttttatcgct aaagatactt cttccacgcg tagaaacgcg cggttttctt gtttcgcacg
      541 ctttacgcac aaaactgact gaagtcatta agaaaataaa agggtctaaa acttttaagt
      601 tttgtaccga cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt
      661 atggctaagt atacttttga acttaaatta aagatcgttc atgattactt ggatggtaaa
      721 ggcggaagtg attatctggc aaagaaatat tccattaagg ctccatcaca agtgaagcgc
      781 tggatcaatg cctatcaaga atttggtgaa gagggtctag taagaaagcg acaaaagaaa
      841 aaatattctg ttcaatttaa gctggatgcg atagagttat atctaacaag tgagctgtcc
      901 tatcgtgaag ttgccaacac acttaacatg aacaacccta atttgatcgc tagttggatg
      961 cgtcaattcc gtgagggtgg gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc
     1021 ttgtctaaga aaaatgaacc caaaaataag aaaaaatcta caactaaagc aacgtctaaa
     1081 gaacgtgaac gcatcgagga gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc
     1141 tttttaaaag aattgaggaa gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa
     1201 tcgcacgaat catcgcaagc ctccgaggac cgtttaaatt agttgagctt ctagaaactt
     1261 taaagttccc taaggcaatc tttatgtact ggcaaaaacg attggaccgt aaaaatccag
     1321 atcaagagat tgaagcggaa atgcttaaag ttcgggagaa gcacaaggat tatggctgtt
     1381 tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc
     1441 gcctgatcaa gaagctagga ataaaagtaa cgacatacac acgtaaatca cggcgctaca
     1501 attcttatcg aggacaagt
//
LOCUS       DAPBBG010000148         1493 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPBBG010000148 DAPBBG010000000
VERSION     DAPBBG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.158"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            185..1057
                     /locus_tag="RD477_002912"
     CDS             185..1057
                     /locus_tag="RD477_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7635170.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1291..>1493)
                     /locus_tag="RD477_002913"
     CDS             complement(1291..>1493)
                     /locus_tag="RD477_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7635171.1"
                     /translation="DNSACEGFFGRLKNEMFYQRDWKNTTINQFINKLDNYIHWYNNQ
                     RIKLSLGGLSPLQYRKKQRYIC"
BASE COUNT          464 a          264 c          316 g          449 t
ORIGIN      
        1 aaatatttta cactctctat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat
       61 aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata
      121 atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat
      181 gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga
      241 tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat
      301 gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggaa ttgggattgg
      361 ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac
      421 cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag aatttccgat
      481 cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag
      541 tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg gcggcggtgc
      601 cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc atatgctcgt
      661 cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca actgtttaga
      721 agggatggcc gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac aagaggttgc
      781 agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg cctacaatac
      841 aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga aacaacgcca
      901 tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg tcgaaacacc
      961 tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa cgctcggttg
     1021 tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct cttcgacatt
     1081 tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag gcagagtgct
     1141 taactggttt ttgttttact tggaagatca agccttgaat gagataaatt cttgctcgaa
     1201 agttatggaa atttcgatag ccgtaggcta cttgtattgt gcaatagttt tttacaaaaa
     1261 aaaggggtgc ggatattttt ttggaccttt ttaacaaata tatctttgct tcttccgata
     1321 ttgaagaggg cttagccctc ctaaagataa cttaattcgt tgattattat accaatgaat
     1381 ataattatcc aatttgttta taaactgatt gattgttgta ttcttccagt cacgttgata
     1441 aaacatctcg ttttttaatc gtccaaagaa accttcgcac gctgaattat ctg
//
LOCUS       DAPBBG010000149         1459 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPBBG010000149 DAPBBG010000000
VERSION     DAPBBG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.159"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            73..483
                     /gene="spx"
                     /locus_tag="RD477_002914"
     CDS             73..483
                     /gene="spx"
                     /locus_tag="RD477_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDT7635172.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            497..853
                     /locus_tag="RD477_002915"
     CDS             497..853
                     /locus_tag="RD477_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDT7635173.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          211 c          269 g          439 t
ORIGIN      
        1 ttattagcta ataattggta ttttaaaaga aattactgta gaattggatt ataattaaaa
       61 aggagtgaga aaatgataaa agtatatact tcgcctagct gtacttcctg tagaaaagca
      121 agagcatggt tgcaagcgaa tcaacttgaa tttgaagaga agaatatttt tgccgaaccg
      181 ttaacagaaa gtgagatcaa aaagatctta gcagcgactg aaggaggagt tcaagatatt
      241 atttccactc ggtcgaaaat ttacgaaaag ctgggcatag attttaacga attaacttta
      301 aagcaaatga tcgtcttatt caaagaatat ccttcattat tgagacgacc tattttgatt
      361 gatgaaaaaa gaatactaat cggatttaat gaagatgaga tccggtcatt tgtccctcgt
      421 gaaattcgat gtgttgccag aaatctgatc tatttacaaa acaataacat cttaaattca
      481 tagggaggga gccttcgtgg aagtgaaagt ctatggatca gcgcactgtg taacaagtct
      541 gaaggcgaaa caatggttag aacaccatca attgccttat caatttgttg acttagaaga
      601 acgcgatcta agtacagaag aagcagaaga attatgcagt tttgaagaag tgcaagcaga
      661 acagctattt gcgacttggt ctgaggcttt tcacaacatt gcgattgacc tttcctgtgt
      721 aggaaaaaaa caactgattt ttttatgcac acaacatacc aatctcctca gacgtccttt
      781 gattattatc gatgaagttc tatttattgg atacaatgag cagttgatgg aacagcttat
      841 cttaaataaa tagactttaa ctgttttaat caagtcactg agcatttcat acagttgtaa
      901 cagtcgaaca cttagcatca agcgtgttat gtgttctttt tttgacttta tttttctact
      961 atagagacat gctcaattta tgtggtcaaa attcaaatct aggtggggat atagagttaa
     1021 aaatatattg acatgtgtaa atttacatga tatattcatt tctgttgttt taagtagcaa
     1081 agcaataaaa tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca
     1141 tgataagata actgagttgt taaaaaagaa ccacttgtga agcttgattc tatcgaaaaa
     1201 aacttcaaaa aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt
     1261 aagatttcga aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc
     1321 tctgatatga tataaaagtt gctacggcag ccaaaaacga atcggggaac actcctgatg
     1381 aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa atttgtaggg cgtcttgatt
     1441 caattcaaga caacaaaca
//
LOCUS       DAPBBG010000150         1455 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPBBG010000150 DAPBBG010000000
VERSION     DAPBBG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.160"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            108..1040
                     /locus_tag="RD477_002916"
     CDS             108..1040
                     /locus_tag="RD477_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDT7635174.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="RD477_002917"
     CDS             1112..>1455
                     /locus_tag="RD477_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635175.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DAPBBG010000151         1424 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPBBG010000151 DAPBBG010000000
VERSION     DAPBBG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.161"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(150..1337)
                     /locus_tag="RD477_002918"
     CDS             complement(150..1337)
                     /locus_tag="RD477_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDT7635176.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAPBBG010000152         1419 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPBBG010000152 DAPBBG010000000
VERSION     DAPBBG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.162"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            86..1381
                     /locus_tag="RD477_002919"
     CDS             86..1381
                     /locus_tag="RD477_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7635177.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          447 a          279 c          267 g          426 t
ORIGIN      
        1 atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga attaatttgg
       61 ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag ataccctaaa
      121 tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa aatacaaagg
      181 gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt gttcccattg
      241 tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc ggttgttact
      301 gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat ttaaatgtaa
      361 gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact gtttcattag
      421 tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca tgacggaaat
      481 cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc gattctaccg
      541 cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg aattcaaatc
      601 ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa gccatgcatt
      661 attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt ctcggttttc
      721 attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc cttatgtttc
      781 gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca
      841 acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg
      901 aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca
      961 aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta
     1021 tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg
     1081 ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga
     1141 ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac
     1201 gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc
     1261 attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa
     1321 cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt taaccaaata
     1381 aacagaacag aaagaggttt tcttctttct gttctgtcc
//
LOCUS       DAPBBG010000153         1358 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPBBG010000153 DAPBBG010000000
VERSION     DAPBBG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.164"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>1358
                     /locus_tag="RD477_002920"
     CDS             82..>1358
                     /locus_tag="RD477_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDT7635178.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          462 a          262 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatca
//
LOCUS       DAPBBG010000154         1337 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPBBG010000154 DAPBBG010000000
VERSION     DAPBBG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.165"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1337
                     /locus_tag="RD477_002921"
     rRNA            <1..1337
                     /locus_tag="RD477_002921"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAPBBG010000155         1311 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPBBG010000155 DAPBBG010000000
VERSION     DAPBBG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.166"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            200..742
                     /locus_tag="RD477_002922"
     CDS             200..742
                     /locus_tag="RD477_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635179.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1248
                     /locus_tag="RD477_002923"
                     /pseudo
     CDS             898..1248
                     /locus_tag="RD477_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAPBBG010000156         1304 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPBBG010000156 DAPBBG010000000
VERSION     DAPBBG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.167"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..>1304
                     /locus_tag="RD477_002924"
     CDS             137..>1304
                     /locus_tag="RD477_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635180.1"
                     /translation="MELLEKAYSSTELIDSYEYEDLKYIKDQRNYSAHPVVTYNGNTW
                     ELRDITKETCQDVIRKSYEIIFLKNPILGKKVTIEIIQYASRIHAMSVTPEEFHSALK
                     NSYLNKLSEKAKENLCKTAFKFVFQLSYGNEEADRDRESTFRLLNALIFDNKEFYLNI
                     LKKEIAKYRFTAESREEINKSLGENQQITYTKGFFLLTFIATHPELQHDFSDETIQNL
                     QISINDFKPKQQVLTEIEYYYRLRSLAVLGNNNEELQNHLNSLVDPILQQKMRVSSLP
                     SSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIYNKLNQEQFNK
                     LLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYPNAVTMDQGF"
BASE COUNT          521 a          193 c          192 g          398 t
ORIGIN      
        1 ttacagtgcg cttattttag atttaattga taaattatct gatttagcaa atatttacga
       61 ggataatact tctaagaaaa ttttagaaga tatagctaga ctaagaaaaa gtgatccaaa
      121 ctctccaaaa tgggaaatgg aactactaga aaaagcttat agttcaactg agctaattga
      181 tagctatgaa tatgaggatt taaaatatat taaggatcaa agaaattatt cagctcatcc
      241 tgtagtcact tacaacggta atacttggga attaagagat ataacaaaag aaacttgtca
      301 ggatgttata cggaagtcct atgaaattat attcctaaaa aatccaatat taggaaaaaa
      361 agtaacgatc gaaattatac aatatgcaag tcgcatacat gctatgtctg ttacacctga
      421 agaatttcac tctgcactaa aaaatagcta tctaaataaa ctaagcgaaa aagccaaaga
      481 aaacctatgt aagacagcct ttaagtttgt ttttcaatta tcttatggaa atgaggaagc
      541 agatagagat agggaaagta ctttcagact acttaatgcc ctaatttttg ataataaaga
      601 attctattta aatattctaa aaaaagaaat tgcaaagtat aggttcacag ctgaatcaag
      661 agaggaaatt aataaatcac ttggtgaaaa tcaacagatc acgtatacaa aagggttctt
      721 tttacttact tttatagcta cccatccaga attacaacat gatttttcag acgagacaat
      781 tcaaaattta caaatatcaa taaatgattt taaaccgaaa caacaagtac taacagaaat
      841 agaatattac taccgtttac gttcccttgc agttctagga aacaacaatg aagaattaca
      901 aaatcactta aatagtctag ttgatcctat actccagcaa aaaatgagag tttcttcgct
      961 accatcttca acattagaaa caatatataa tcaatattta tattttggtg aaaaagagat
     1021 gttcatagat tttatgatcg aacatcttac cgatgctagt tcattcaggt tagcagataa
     1081 ggatatggaa gtattaccat ggatttacaa taaattaaat caggagcaat ttaacaaatt
     1141 actatacaac ctaaatggaa ataatcaatt ttacaaaaac agtaattttc cgccttttat
     1201 ttctaatctg ttagactttt ataatcagaa attccaagtg aatttacgta aacattatag
     1261 tgggcttcta tatccaaatg cagtgactat ggatcagggt tttc
//
LOCUS       DAPBBG010000157         1282 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPBBG010000157 DAPBBG010000000
VERSION     DAPBBG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.168"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            81..1253
                     /locus_tag="RD477_002925"
     CDS             81..1253
                     /locus_tag="RD477_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDT7635181.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          478 a          235 c          227 g          342 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatcttta ccaccattcc taaaaaaaat tcaaaatctt
      901 tcagagaagc tgttaaagga atttttaagt tcacagatat taacttagcg cgtgaggcta
      961 aaaatcgatt gattcatgat tatatcgatc aaccaaaata ttcaaaagct tgcgcatcat
     1021 tggatgatgg attcgaagac gcctttcaat ataccgtaca aggaaattcc cacaatcgac
     1081 taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga gaaaagatta
     1141 ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt cttatggacc
     1201 tacatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag tagaaatggt
     1261 aaaaacattg tatagcattt ta
//
LOCUS       DAPBBG010000158         1203 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPBBG010000158 DAPBBG010000000
VERSION     DAPBBG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.169"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1153
                     /locus_tag="RD477_002926"
     CDS             <1..1153
                     /locus_tag="RD477_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDT7635182.1"
                     /translation="REEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLI
                     SNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDP
                     LGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAG
                     DVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQ
                     AVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQ
                     YACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVY
                     KTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          389 a          217 c          258 g          339 t
ORIGIN      
        1 acgagaagaa attcgtttcc atcaggctat gttggattgt ctcacccatt atcaacatac
       61 ctattgttgt aaaagctgcg aaaaagaagg actttcttct tttaaaaaag cgatcgttcc
      121 taaacctttg atttctaata gcttaggttc caatagttta gtggcggaaa cgatccggat
      181 gaaatttggt cagaaagttc ctgcctatcg gcaagagaac tattggaaac aaacacatgg
      241 gttggatatt tcgagagata atatcaccaa ttggcatatt aaagcagtgc aaaacgcgtt
      301 agacccttta ggagaacgat taagagtcta tcttaatcaa gaagagattc tacacgggga
      361 tgaaacaagt tatcgtgtga ttgaaagtgc caaaacagat acctattact ggcagttttg
      421 tacaggaaaa gacagtcagc atcccatcgt ttattatcac cacgacgaaa gtcgtgctgg
      481 agatgttccg aaaacttttc tcaaggagtt tacagggtat ttacactgtg acggctatag
      541 cgggtataat gccgtggaaa gtgtacgact tgtctattgt ttcgcgcatg ttcggcggaa
      601 atttttcgaa gcaattccaa aaggaaagaa aaacacggat attcccgcag ctcaagcagt
      661 caaacaattg gataaatggt ttgtcctaga aaagaaatgg aaagactttt ctcctgagaa
      721 gcgattaagc tgtcggcaac aagaactgcg tccattattt attgcttttt atgagtggat
      781 ggcaacgata gatcctgttg caaaatccaa gttggatgca gccgttcaat atgcgtgtaa
      841 actacgaagt ggttttgaac ctatttttga agatggtcgt ttagaactca caaataatcg
      901 tgcggagcga aatatcaaag agctggtgat aggacggaaa aattggttgc attccacaag
      961 tcttgaagga gcacgaactt caggaatcat tctgagtgta tataaaacag cagaacttaa
     1021 cggattgaat cctgtaaagt atctagagtt tctatttgat aagataccaa atcttcccgt
     1081 actttcagca gaaactttag atcaactgtt gccttggaac aaggatgtac aacaacattt
     1141 ttctcgtaac taaaaacgcc aaacattccc gattctattc tataggtcgg gttatttgac
     1201 gta
//
LOCUS       DAPBBG010000159         1068 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPBBG010000159 DAPBBG010000000
VERSION     DAPBBG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.171"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..373)
                     /locus_tag="RD477_002927"
     CDS             complement(<1..373)
                     /locus_tag="RD477_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7635183.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQM"
     gene            complement(475..828)
                     /gene="tnpB"
                     /locus_tag="RD477_002928"
     CDS             complement(475..828)
                     /gene="tnpB"
                     /locus_tag="RD477_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDT7635184.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(818..1012)
                     /locus_tag="RD477_002929"
     CDS             complement(818..1012)
                     /locus_tag="RD477_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635185.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          302 a          213 c          188 g          365 t
ORIGIN      
        1 tcatctgtga accgcaatgt tcacaaaatt gttcttccaa aggaaggaga tgttctttct
       61 ctgttatagg taagtgtttg attttgtctg ctttcgttcc tacaactcgt tgatgtcctt
      121 ttactggaat aatggtggca gctgcttctg gaacacttac agaaatttct tcatcactaa
      181 aaagattcaa ttgcccattc gtttcctcaa agagagtctc tttagagcgt ccaaataatt
      241 tgttggttaa ataattgacc ttttcattca atagttggat ttcagttgta agattctgaa
      301 tggtttgttg ttgtgcttga ttggttttat gggcttcttg aagcaactga agcaacgtat
      361 ctgtagtttc caaaattttt caccaccttg aatcgaactg attttgttac agaataccac
      421 aagaaacgca caagaaatag gggttcaaca cattttttgt gttaaccccc cagcttaaaa
      481 aactcctttt tttcctggca aaatagctcg aggttgatcg atacttaatc cttctaacaa
      541 ccatcgaacc tgttggttcg ttaaggttcg aacttcgtct ttcgtccgag gccattttaa
      601 cttgccgttt tctatccgct tataacagag aagaaaacca tcaccgtccc aatagagaag
      661 tttgaaacgg tcttgacggt tcccgcaaaa taaaaaaacg gcatcttcgt aaagctcgag
      721 ttgatactct tgttgaataa gactggcaag accatcaatt ccttttcgca aatctgtttt
      781 tccgcacaca atataaatat gtttggtttg aagtaaatca taaagcattg gacaacagtt
      841 ctttcaaaat agcttgaaga atataattgt taataccgtt atagatcaaa acttcagaac
      901 cattctctct tttgatgcgc atcgctaact tggcagccgc gggtttacct ttttctggtg
      961 cactcttttc gtttcctaat tggacaggaa caatctcatt taatttatac atagaaaacc
     1021 tccagacttc ttgatagctc aagtatatct tgaggtgcat ttgttagt
//
LOCUS       DAPBBG010000160         1061 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPBBG010000160 DAPBBG010000000
VERSION     DAPBBG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.172"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(36..992)
                     /locus_tag="RD477_002930"
     CDS             complement(36..992)
                     /locus_tag="RD477_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635186.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DAPBBG010000161         1047 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPBBG010000161 DAPBBG010000000
VERSION     DAPBBG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.173"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..463
                     /locus_tag="RD477_002931"
     CDS             <1..463
                     /locus_tag="RD477_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635187.1"
                     /translation="TTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVGYVSTKLIKRSI
                     EKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSILKNGQEMKAI
                     DRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMKDK"
     gene            484..767
                     /locus_tag="RD477_002932"
                     /pseudo
     CDS             484..767
                     /locus_tag="RD477_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          399 a          126 c          217 g          305 t
ORIGIN      
        1 aacaacaaaa ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat
       61 gtatttgttt gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat
      121 taaacgttca attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga
      181 gtacaatgtt gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag
      241 tatccatgaa tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa
      301 agcaatagat cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata
      361 tggcatcgga aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt
      421 ttggaacagt agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg
      481 aaaatgattc ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag
      541 aaggagcagt tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt
      601 acgatgaaca ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
      661 ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg
      721 atgaatggga acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg
      781 taaagagcat aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc
      841 tattacgttt ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg
      901 atagtgaagg atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc
      961 tatccttttt aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa
     1021 atcgtaagag aaattgatcg aagatgt
//
LOCUS       DAPBBG010000162         1035 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPBBG010000162 DAPBBG010000000
VERSION     DAPBBG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.174"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>1035)
                     /locus_tag="RD477_002933"
     rRNA            complement(<1..>1035)
                     /locus_tag="RD477_002933"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAPBBG010000163         1013 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPBBG010000163 DAPBBG010000000
VERSION     DAPBBG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.175"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(73..303)
                     /locus_tag="RD477_002934"
     CDS             complement(73..303)
                     /locus_tag="RD477_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635188.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(397..735)
                     /locus_tag="RD477_002935"
     CDS             complement(397..735)
                     /locus_tag="RD477_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635189.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          329 a          203 c          109 g          372 t
ORIGIN      
        1 aatattaaca gcaaagatat cgaaaaaaca ataacataat atgtgtcaaa atttttcata
       61 ataagaactc ccttactgtt gcatactaac ataatccaaa aactcttcta cttcagctaa
      121 agacattcca ctttctttag tccaaaaaac agttttttct atcgatcctt ctctgtaaac
      181 tgtaaatgtc ggagtcgagt taattgagta gcgactcttg aattctttcc actcattttc
      241 atcttctcta acttttgcta cattcacgta ataaatattt tcttttccct tttctaccag
      301 catatcgaat agcttcctgc ttttgatgat cgatttcttg tgctacatct agtaaatttt
      361 catcaatttc tatatcataa agtatttcac tagtgtctac atccacaata tatacttttt
      421 atcagtagca atttttgagt aatccgttaa aatgatctca gtctcgtcac tttcatcagt
      481 ataaatatac gttaacaatg aactcacctt ttcaatcggt gttagctcta agcctactgt
      541 attttttact attgagtcat tatttaccac cataacttcg tactgtatat accatcctat
      601 ttggtttcca tcaatatcag taattggctc actagctaat ggagtctccg gaacaatatt
      661 ttcctgatat ttcttaaact cttctgtagc acgaaaaagg ttatcatgtt cctcacttga
      721 aactggttga gtcatagaaa atgttacttg gctaaacaga gggaaaataa acactacaat
      781 caacaaagta taaaaaaaag cccttctttt tcatttttta tcttccaatc acaaattgaa
      841 aaacttattc ctgcaacttt gagtctagta tcacaatcga ctgactacaa ctattcattt
      901 acattctact ttaagttcct ccttaaccat taattttatt aaagatactt gtaatagtga
      961 ttctcacaaa atttcatgaa aaatacttta ttactgtatc taaaattcca ata
//
LOCUS       DAPBBG010000164          972 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPBBG010000164 DAPBBG010000000
VERSION     DAPBBG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.176"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            89..781
                     /locus_tag="RD477_002936"
     CDS             89..781
                     /locus_tag="RD477_002936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635190.1"
                     /translation="MNIITRDELIYAYKDEVNLLEVSQYRSSNVITLNIDTVSEFIKL
                     AKELKINHVYYNYLYYSIDNYLIPLDTYDEFSDEINIEVKKHNEVVDSLDFSKPKQLT
                     LLIVLNGTIMEITLNHFWIKESGIPDNETKHSEIQDKFFKDFKLQQEEISRNEHDDKN
                     RLKEYILSDNEFSYMKNQRLRQDYLYDLLSKDKFEKFNYLFEGDFDRGISIPIKHYMD
                     RIWQEYKERRKK"
BASE COUNT          385 a          118 c          154 g          315 t
ORIGIN      
        1 aaattattca tctttaattc tgaaaaatta ataattgatt gaagtgatat ttttggatta
       61 tataataatc gggtaggaag tggagttaat gaatattata actagagatg aattaattta
      121 tgcatataaa gatgaggtca atttattgga agtgagtcaa tatcgatcct caaatgtaat
      181 tactttaaat attgatacgg tatcggagtt cataaagtta gcaaaagaat taaagattaa
      241 tcatgtatat tataattatc tatactattc tattgataat tacctcatac ccttagatac
      301 atatgatgaa ttttctgatg aaatcaatat agaggttaaa aagcataatg aagtggtaga
      361 tagtcttgat ttttctaaac caaaacaact aaccttacta attgtactga atggtactat
      421 aatggaaatt actttaaatc atttttggat aaaggaaagc ggaataccag ataatgaaac
      481 taagcattct gagatacaag acaaattttt caaggatttt aaacttcaac aggaagaaat
      541 atctagaaat gaacacgacg ataaaaatag attaaaagaa tatatattga gcgacaatga
      601 gttctcttac atgaaaaatc aacgcctgag acaagattat ctctacgatt tactttcgaa
      661 agataaattc gagaaattta attacctgtt tgaaggtgat tttgaccgag gcatctcgat
      721 tcctatcaag cattatatgg atcgaatctg gcaagaatat aaggaaagaa gaaaaaaata
      781 gcgcatttta agtcgatttc gaatatcaaa gtggacaact atccacgaaa taggcttaaa
      841 acgcgtttta tttcatttga atttatagct tttatgaaga aaatccttac tgacttataa
      901 acataagata taagtaggac aaatacgcac taagtattga ttatcaatac atggtcatta
      961 atcttaagtt tt
//
LOCUS       DAPBBG010000165          943 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPBBG010000165 DAPBBG010000000
VERSION     DAPBBG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.177"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>943
                     /locus_tag="RD477_002937"
     rRNA            <1..>943
                     /locus_tag="RD477_002937"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAPBBG010000166          928 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPBBG010000166 DAPBBG010000000
VERSION     DAPBBG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.178"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            55..928
                     /locus_tag="RD477_002938"
                     /pseudo
     CDS             55..928
                     /locus_tag="RD477_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          362 a          153 c          178 g          235 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattatt
//
LOCUS       DAPBBG010000167          911 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPBBG010000167 DAPBBG010000000
VERSION     DAPBBG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.179"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            70..>911
                     /locus_tag="RD477_002939"
     CDS             70..>911
                     /locus_tag="RD477_002939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635191.1"
                     /translation="MNKKEIKEFVKANRWFTYEQAENELRKHWNSDCDKDGDYLKIKR
                     RQIQKILRSDIIGTYLEMDKRDVGITKEEIFMKKLYGWTAEETFYLEYYDSERDDIIE
                     EEYTEKLHVFPKYDRLFPNNESSMILSTWDEQLGEDEYTAMKVFMREVYEKVFQLDTR
                     GNTAYIMNESYLFALKHELERREYPTKIVYLKPHSPKEITKNLSNTKHDYDILQNSDI
                     LLKDFNEQIENLVITHNKAKDVEIERYFNIDNFFIKWQNTYPEAINRIISSLKEKELF
                     EKFF"
BASE COUNT          379 a           87 c          168 g          277 t
ORIGIN      
        1 ttaaattatt agtctacttg tgtattatgg tcttcataag aaaaatgaaa ttgaatagga
       61 gcagaagtga tgaacaaaaa agagataaaa gagtttgtta aagccaatag gtggtttaca
      121 tacgaacaag ccgaaaatga attaagaaaa cattggaatt ctgactgtga taaagatggt
      181 gattatttaa agataaaaag aagacaaatt caaaaaatct tacgttcaga tattataggg
      241 acatatttag aaatggataa aagagatgtt gggattacta aagaggaaat atttatgaag
      301 aaattatacg ggtggactgc ggaagaaacc ttttacttag agtattatga ttcagaaaga
      361 gacgatatta ttgaggaaga gtacacagag aagcttcatg tttttccaaa atatgatagg
      421 ttgtttccta ataatgaaag tagtatgatt ctttctacgt gggatgaaca gttaggggaa
      481 gatgaatata ctgcaatgaa agtttttatg agagaagtat atgagaaagt atttcaatta
      541 gatactaggg gaaatactgc atatattatg aatgaatcgt atttatttgc attgaagcat
      601 gaattagaga gaagagagta tcctactaaa atagtgtatt taaaacctca ctcacctaaa
      661 gaaataacta aaaatttatc gaacactaaa catgattatg atattttaca aaattcagat
      721 attcttttga aggacttcaa tgaacaaata gaaaatttag taattacaca taacaaagct
      781 aaagatgttg aaatagagcg atattttaat attgataact tttttattaa atggcaaaat
      841 acttatccag aagcaataaa tcgaattatt agtagtttaa aggaaaagga attatttgaa
      901 aaattcttca a
//
LOCUS       DAPBBG010000168          890 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPBBG010000168 DAPBBG010000000
VERSION     DAPBBG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.180"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..774
                     /locus_tag="RD477_002940"
     CDS             <1..774
                     /locus_tag="RD477_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDT7635192.1"
                     /translation="HFLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAE
                     LPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIR
                     ALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAK
                     YAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEV
                     PKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          318 a          173 c          157 g          242 t
ORIGIN      
        1 cattttcttg aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa
       61 tcgacgagtc tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat
      121 gaacttgctg agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc
      181 cgtttcaagc aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac
      241 cgcctaggtg ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa
      301 atacgagcac ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt
      361 gtcattccag aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc
      421 ggtttaatcg ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa
      481 tacgctggat taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct
      541 ctgacaaaac aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta
      601 aaaaattatc ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag
      661 catcaacaca aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg
      721 ctactacgta aacaccaact ctatacgcca ccaaggagtg tgatagagaa ataacaatcc
      781 gttattgacg caaatccttg aatcaacccg aaaaaaattt gattttgatc gggtctagtt
      841 tcgtgcgctt caaaacatat tactcaataa agaaaatctt aaatttcatc
//
LOCUS       DAPBBG010000169          863 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPBBG010000169 DAPBBG010000000
VERSION     DAPBBG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.181"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..861
                     /locus_tag="RD477_002941"
     CDS             <1..861
                     /locus_tag="RD477_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDT7635193.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DAPBBG010000170          811 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPBBG010000170 DAPBBG010000000
VERSION     DAPBBG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.182"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..672
                     /locus_tag="RD477_002942"
     CDS             <1..672
                     /locus_tag="RD477_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7635194.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAPBBG010000171          796 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPBBG010000171 DAPBBG010000000
VERSION     DAPBBG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.183"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..720
                     /gene="aph(2'')-I"
                     /locus_tag="RD477_002943"
     CDS             <1..720
                     /gene="aph(2'')-I"
                     /locus_tag="RD477_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDT7635195.1"
                     /translation="KAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTP
                     EIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYND
                     LTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGII
                     DEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIK
                     NIKQEFIENGRKEIYKRTYKD"
BASE COUNT          350 a           52 c          127 g          267 t
ORIGIN      
        1 aaagcaatat ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt
       61 gaatattcgt atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact
      121 tttttaacac cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga
      181 gatattgcca gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt
      241 actattgata ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat
      301 aatgatttaa ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca
      361 acaacagttt ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta
      421 ttgttagatg gcaataatag attaactgga ataattgatt ttggagattc tggaattata
      481 gatgaatatt gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat
      541 tttggagaag atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat
      601 caagatatag ttgaagaata ttatcctatt gaaactattg tttatggaat taaaaatatt
      661 aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata aaaggactta taaagattga
      721 ttatataata tatgaaaagc tattataaaa gacattagta ttaaatagtt taaaaaaatg
      781 aaaaataata aaggaa
//
LOCUS       DAPBBG010000172          792 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPBBG010000172 DAPBBG010000000
VERSION     DAPBBG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.184"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..781
                     /locus_tag="RD477_002944"
     CDS             <1..781
                     /locus_tag="RD477_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7635196.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          107 c          102 g          244 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgcccct tt
//
LOCUS       DAPBBG010000173          787 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPBBG010000173 DAPBBG010000000
VERSION     DAPBBG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.185"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            39..554
                     /locus_tag="RD477_002945"
     CDS             39..554
                     /locus_tag="RD477_002945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7635197.1"
                     /translation="MITSVEGDQVLEVGVGTGKNLLLYPEKLEITGIDFSEKMLEKAI
                     QKTKGKENITLIEMDAQNMSFADNTFDTVVTSCVFCSVPDPVKGLKEIRRVCKPNGKI
                     VMLEHMRSHHTIIGKFMDMANFFPLYTWGANINRETISNILKAGFKEEEIETQDLWSD
                     IVKLIEIRNKK"
BASE COUNT          309 a           94 c          150 g          234 t
ORIGIN      
        1 cgatggaagc tatgacaatg agcacatgga gagaaaaatt gattacatct gttgagggag
       61 atcaagtttt agaagttgga gttggtactg gaaagaactt acttctttat ccagaaaaat
      121 tggaaataac tggtatagat tttagcgaaa agatgttgga gaaagcgatt caaaagacaa
      181 aaggaaaaga aaatattaca ttaatagaga tggatgccca aaatatgagt tttgcggata
      241 atacctttga tacagtcgtt acttcttgtg tgttttgctc tgtgccagat cctgtaaaag
      301 ggctaaaaga aataaggaga gtctgtaaac ctaacggtaa aattgttatg ttggaacata
      361 tgagaagtca ccatactatc attggaaaat ttatggatat ggcgaacttt ttcccgctat
      421 atacttgggg tgctaatatt aacagagaga caatttcaaa tatattaaaa gctggattta
      481 aagaagaaga gatagaaact caggatttat ggagtgatat tgtaaaatta atagaaataa
      541 gaaataagaa ataaaggtgt agagaccttt atgaaaaaaa gactttatct ataaagaact
      601 aatactatcg gaagaaacga tcttaaaagt gcttcttccc gaagcttata aaattcaaga
      661 tactttatcg gtgaaaaaaa tcatttttta aatatcaatt tatttaaaga tatgtttacg
      721 tctaaggcgt aagcacatct ttatttttta ttcccacatt aaaatagata aaacctgata
      781 atcaaaa
//
LOCUS       DAPBBG010000174          765 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPBBG010000174 DAPBBG010000000
VERSION     DAPBBG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.186"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            660..>765
                     /locus_tag="RD477_002946"
     rRNA            660..>765
                     /locus_tag="RD477_002946"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          315 a          124 c          134 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaaga
//
LOCUS       DAPBBG010000175          750 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPBBG010000175 DAPBBG010000000
VERSION     DAPBBG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.187"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(85..516)
                     /locus_tag="RD477_002947"
     CDS             complement(85..516)
                     /locus_tag="RD477_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDT7635198.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          256 a           98 c           85 g          311 t
ORIGIN      
        1 atttttttag gaatgtaaag tcttatctct tttcttaatt gtacaattag tattatctgc
       61 actactttct aattaaataa atttttagtt attaacttga ttaaaatcag agataatatc
      121 atttaattca gtttgaaggg ttgtaacgat taaatggaaa ttttcttcat ctgttttatt
      181 atatggggat gttttcgtta tatataacaa cttttctttt tcaattttca ttaataactc
      241 tgtcgaagtc tgtactttat cttgataatt aaatgcagac atcccacttt gtataattgt
      301 aattattgct gaagcaattg aaacaatcaa tagtaaagac ctgtgttcgg aagcctgatc
      361 aattaatatt gggatactag ctgataagac tatttttata atatttccga taccaattac
      421 tctattataa aatcgaattt ctttttttaa tctatcgatt gtagaatcaa tagattcgat
      481 aaacgctttt tgatccatca ttttctctcc aatcatttta ttaaaatcat taacaaaaat
      541 attataatag gatttaagta agttctaact aatcacatat aaaaataaaa aaacatttgt
      601 attttatcaa agttttatta tacttgtcaa tttgtgtaca tcctaaaata taagctattt
      661 gtttacttct gtagaatata tactttgttg ttagataagt tgcacatgtt tatagaatct
      721 aaataataaa tttttatgta ccgcccctca
//
LOCUS       DAPBBG010000176          733 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPBBG010000176 DAPBBG010000000
VERSION     DAPBBG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.188"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            163..>733
                     /locus_tag="RD477_002948"
     CDS             163..>733
                     /locus_tag="RD477_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDT7635199.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT          272 a          101 c          141 g          219 t
ORIGIN      
        1 aatttctctc aaaaataaag gagaagttgt attgtttgct tttaagaaag ccctgaaaaa
       61 tttgttacgt actaaattgt ctgaactctg tcatatcagc tatgtcagaa taaaggaaat
      121 agatttattt ttataaacag tgtcgtctcg gagatttaaa ctatgtcaac gacaattgaa
      181 gtgaacaaac aaagcgtgaa gcagtttctt gaaacaggga aaatcaaaaa attcgttatt
      241 ccagaatatc aacgtcctta tgcctggact gacgagcaaa accaagtact atttgatgat
      301 ttagcggagt acacagcaaa caataacgag agtacttact ttttaggaag cattgtggct
      361 tatgaaaatg atcataacga gcaagaaatc attgatggac agcaaaggat aactacgcta
      421 tttttatttt tgagagctat ctatgctaaa ttagaaaata gttgtgaaaa ggaagcttta
      481 ttcttgaaaa gccaaattga acccgcgtta tgggaacaag atgatttaac aggtgaggtt
      541 aaaccagata aaattctgat tatgtctcga gtaatggggg atgaggggaa tgaagaattt
      601 gctagtattc tagtaagtgg tgaagcagat gttaaatcaa aaagtaatta ttcgaagaac
      661 tatattttaa ttcaacatct attaaatgag tatgcaacaa acgaaccact atcattttat
      721 cgttttatca gta
//
LOCUS       DAPBBG010000177          723 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPBBG010000177 DAPBBG010000000
VERSION     DAPBBG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.189"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(51..422)
                     /locus_tag="RD477_002949"
     CDS             complement(51..422)
                     /locus_tag="RD477_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635200.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          290 a           99 c          117 g          217 t
ORIGIN      
        1 ttttaaaagt ttttccttaa atgtgtcttt attgatacag agaagcttct ttaataaaac
       61 aatgcctccc taaattttct tgagatagga aaggtgaaca tacaaaataa tacgaccact
      121 ttgaaaaccc agatgatcca caaatggatc gtatgatact gatgaagtaa aaagcaatgg
      181 atcagaaact caaagagatt gaaaaagaca aggaagaaat aatatctctt ttttactctt
      241 gtctgtttta gccaataata gctcgtaaag taatctagac aaacaaatag aagtaaacaa
      301 agtttcgtaa gagcagcaat cgtatcatgt tgctctgcta gacgattgac gagcaggaaa
      361 aatgttggta atggaatata gatcacgagt aatgaaagaa aaaagtaaac aataaatggc
      421 atactattcc tcctaattgt gtgagttcgc cctcatttta aaataacgat atttgtttgt
      481 gaagcttttt cgtgtcgtaa caaatgttat ctaatgtatt tttaaataaa taatcattat
      541 tatcaaaata atgttaaaaa taaaaaagaa ttgtccatca ctgttattca aatcatttta
      601 tcaaaactac atagtaaatg ttaaaaagca acatgacata atatatagaa ggctttacag
      661 tatatatggg agcttactaa aaaagaatgg agtaagagtt aacacaatgg gccattagct
      721 gaa
//
LOCUS       DAPBBG010000178          691 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPBBG010000178 DAPBBG010000000
VERSION     DAPBBG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.190"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          238 a          113 c          106 g          234 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc acaatttaac
      481 ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact
      541 atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt
      601 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta acacagaacg
      661 attcaaatgg ctacgttaat gtaccctgta g
//
LOCUS       DAPBBG010000179          685 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPBBG010000179 DAPBBG010000000
VERSION     DAPBBG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.191"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            42..>685
                     /locus_tag="RD477_002950"
     CDS             42..>685
                     /locus_tag="RD477_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HDT7635201.1"
                     /translation="MCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRR
                     FQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRL
                     AYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQY"
BASE COUNT          250 a          106 c          145 g          184 t
ORIGIN      
        1 tggaaattat aatcaaacag aaagtagagc tgcaccctaa tatgtgtgtg atgagctgta
       61 cggaacctga gcaatgggat gtggtaatag aaggaaatga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattagaac tcgaacaaga ggaaatcaga agatttcaga
      181 attgctcctt gtaccctgtt tatcatggaa gcgcaaaaag caacataggg attgagcagc
      241 ttatagaagt gataacgaat aaattttatt catcaacata cagaaagaag tctgaacttt
      301 gcggaaatgt cttcaaaatt gaatattcgg aagaaagaca acgtcttgca tatgtacgcc
      361 tttatggcgg aatcctgcat ttgcgggatt cggttagaat atcggaaaag gaaaaaataa
      421 aaattacaga aatgtatact tcaataaatg gtgaattatg taaaattgat aaggcttatt
      481 ccggggaaat tgttattttg caaaatgagt ttttgaagct aaatagtgtt cttggagata
      541 caaagctatt gccacagaga gagagaattg aaaatccgct ccctctgctg caaacaactg
      601 ttgaaccgag caaacctcaa caaagggaaa tgttacttga tgcactttta gaaatctccg
      661 acagtgaccc gcttctacaa tatta
//
LOCUS       DAPBBG010000180          630 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPBBG010000180 DAPBBG010000000
VERSION     DAPBBG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.192"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          242 a           81 c           79 g          228 t
ORIGIN      
        1 aagaaggtat caagagtcac agtgaattta ttccgaaaag tgaaaagagc aaatataaaa
       61 tttaataaaa atatagaact aaataggaaa acgtagacgt aattttctac gttttccttt
      121 tcatatatat taaatattac ggaaaattat ataaactaat ttagtgtttt gtttgtaatt
      181 cctaattttg ctccactttt agataatttt taggtctatt taactcatca gacattctat
      241 gctcattttc tccaaatttg tttcaactct ttcgtactta attttgcgta tttgaaaatt
      301 cttcttttag agtactttta ttacgatact agtatgatat atatctatag caaacagtta
      361 aaatcttcct aaaataagcg aatagcattc ctaaaaccta taaaattttt agatgaagcg
      421 acaagtggac aattaataaa ctacttgaac atgaaattat ggatcacctt tcaaatttag
      481 aaaatggaat tcttatactt attgcacaac atttattgat tgcaaaatta tgtgacaaaa
      541 agaactagta gaagaaggaa taatggcttg ataataaagt actacctatt aaaggtagta
      601 ctttatttaa tttagtataa attaacatac
//
LOCUS       DAPBBG010000181          622 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPBBG010000181 DAPBBG010000000
VERSION     DAPBBG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.193"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(95..574)
                     /locus_tag="RD477_002951"
     CDS             complement(95..574)
                     /locus_tag="RD477_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635202.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          202 a           94 c           68 g          258 t
ORIGIN      
        1 tgcttttcac taattacaaa atatgtgata taggaagttt agttattgtt ctcataatgc
       61 catcctgttt ttagattatc tagtaatttt tttgttattt aaaattagga gcagtgccaa
      121 ttgttttaat tttaaaggct ttttttatat cttctaaatt agaagatatt tgtatatcaa
      181 tatggaatat attacctttt ttgtattttt ttatgttatc tttagctaaa acatcaaata
      241 catgtccttg aaattgtatc gaggcagctg gatttagttt aaattgagga aactttacca
      301 cagctgattc aaaacacttg tcacattttt ttattttttt acctttgtaa aaatattcga
      361 ttgaatattc tatttggcac gactcacttg gcagtatatg tatgtcaata tttttcatct
      421 gtttcatagg tatttcaaaa tctaaactat taaaaacata taattccctg taattattta
      481 catctaactc tactaaattt atctttggaa caatagatcc tccacccatt ccaccataat
      541 aaaaaaaatc ttgttctcta tcaaaatagc acatattaag ctcagtttgt ttatcatcct
      601 caataatttt ataaatcaaa cc
//
LOCUS       DAPBBG010000182          586 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPBBG010000182 DAPBBG010000000
VERSION     DAPBBG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.194"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..546
                     /locus_tag="RD477_002952"
     CDS             <1..546
                     /locus_tag="RD477_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDT7635203.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT          207 a          100 c          110 g          169 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttct
//
LOCUS       DAPBBG010000183          581 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPBBG010000183 DAPBBG010000000
VERSION     DAPBBG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.195"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            312..>581
                     /locus_tag="RD477_002953"
     CDS             312..>581
                     /locus_tag="RD477_002953"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDT7635204.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTI"
BASE COUNT          197 a          107 c           83 g          194 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacgat c
//
LOCUS       DAPBBG010000184          568 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPBBG010000184 DAPBBG010000000
VERSION     DAPBBG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.196"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>568
                     /locus_tag="RD477_002954"
     CDS             <1..>568
                     /locus_tag="RD477_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7635205.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTN"
BASE COUNT          234 a           50 c          100 g          184 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataa
//
LOCUS       DAPBBG010000185          557 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPBBG010000185 DAPBBG010000000
VERSION     DAPBBG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.197"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            58..>557
                     /locus_tag="RD477_002955"
     CDS             58..>557
                     /locus_tag="RD477_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7635206.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGR"
BASE COUNT          188 a          101 c          109 g          159 t
ORIGIN      
        1 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
       61 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      121 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      181 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      241 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      301 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      361 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      421 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      481 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      541 aaaccgtgtg gtagaca
//
LOCUS       DAPBBG010000186          556 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPBBG010000186 DAPBBG010000000
VERSION     DAPBBG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.198"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..154
                     /locus_tag="RD477_002956"
     CDS             <1..154
                     /locus_tag="RD477_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635207.1"
                     /translation="IKLKADLPLLNSTQIDRHENIKVNIEKGALAQLYTEKVEQFFEA
                     GANKDF"
     gene            221..520
                     /locus_tag="RD477_002957"
     CDS             221..520
                     /locus_tag="RD477_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635208.1"
                     /translation="MAEDMYNMTLKKIKSYDHPLNLTLNKGILSFVLAGYVDYEYTAD
                     YLETPLQHDNNDINFRFIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
BASE COUNT          220 a           82 c           86 g          168 t
ORIGIN      
        1 tataaaacta aaagctgatc ttcccttact gaacagcaca caaattgata gacatgaaaa
       61 tattaaagtt aatattgaaa aaggagcgtt agcacaactt tatactgaaa aagttgaaca
      121 attttttgag gctggtgcca ataaagactt ttaaaaaata agcgattatt cggataactt
      181 ttttaatgag acccaagatg aaatcacgaa ctcaactttt atggctgaag atatgtacaa
      241 tatgacgttg aaaaaaataa agtcgtatga tcatccatta aatctaacat taaataaagg
      301 gatactctct tttgtattag caggttacgt tgactatgaa tatacagcag attatttgga
      361 gacgcccctt caacacgata ataacgatat taattttaga tttatctaca attcaaaaag
      421 taaaacatgg ttagttgatc aattgagcac tataaatacg ggatttcatg aattacctaa
      481 gcctgacaat gaaactgctt tggtcattaa aacattctaa aaaagaaacg ttgaaggaat
      541 aattattctt tcaacg
//
LOCUS       DAPBBG010000187          519 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPBBG010000187 DAPBBG010000000
VERSION     DAPBBG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.199"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            10..375
                     /locus_tag="RD477_002958"
     CDS             10..375
                     /locus_tag="RD477_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7635209.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            376..>519
                     /locus_tag="RD477_002959"
     CDS             376..>519
                     /locus_tag="RD477_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HDT7635210.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKK"
BASE COUNT          211 a           36 c           88 g          184 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatggt
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaa
//
LOCUS       DAPBBG010000188          514 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPBBG010000188 DAPBBG010000000
VERSION     DAPBBG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.200"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..132)
                     /locus_tag="RD477_002960"
     CDS             complement(<1..132)
                     /locus_tag="RD477_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDT7635211.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAPBBG010000189          488 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPBBG010000189 DAPBBG010000000
VERSION     DAPBBG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.201"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>488
                     /locus_tag="RD477_002961"
     CDS             <1..>488
                     /locus_tag="RD477_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635212.1"
                     /translation="RKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTD
                     AKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNK
                     EDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPT
                     VEG"
BASE COUNT          197 a           73 c          114 g          104 t
ORIGIN      
        1 cgtaaagtaa cctttactgc aggagaagga acaagtattg aaagtggaac aacagtcttt
       61 gcagtgaaag acggcgtaag cttaccagaa gataaacttc cagtgttgaa agcaaaagat
      121 ggttatacag atgcgaaatg gccagaggaa gcaactcaac caattacagc agatgataca
      181 gaatttgtat caagtgcaac aaaactggat gataaatctg acgctgacaa atataatcct
      241 gaaggtcaaa aagtgactac agaattgaat aaggaaccag acgcatttga gggaattaag
      301 aacaaagaag atttaccaaa agatactaag tatacttgga aagaaaaagt agatgttagc
      361 gcagctggaa ataaaaaagg gactgttgta gtgacatatc cagatggatc atctgatgaa
      421 gttgaagtag atgttacagt gacagacaat cgttctgacg ccgataaata tgagccaaca
      481 gtagaagg
//
LOCUS       DAPBBG010000190          462 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPBBG010000190 DAPBBG010000000
VERSION     DAPBBG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.202"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..430)
                     /locus_tag="RD477_002962"
     CDS             complement(<1..430)
                     /locus_tag="RD477_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7635213.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAPBBG010000191          456 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPBBG010000191 DAPBBG010000000
VERSION     DAPBBG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.203"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          151 a           72 c           71 g          162 t
ORIGIN      
        1 gtcaagattt acctgaagat catccttttt aggaaattga agctttttat gttgcacaat
       61 gcatttataa tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga
      121 aacagataca cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg
      181 ttaatcttcc gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta
      241 aggttggcaa aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt
      301 tggttgacac ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt
      361 acctaaaaag tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat
      421 ttcaacttat atgttttagt gaactacccc cactta
//
LOCUS       DAPBBG010000192          450 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPBBG010000192 DAPBBG010000000
VERSION     DAPBBG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.204"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          143 a           90 c           68 g          149 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata
//
LOCUS       DAPBBG010000193          426 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPBBG010000193 DAPBBG010000000
VERSION     DAPBBG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.205"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>426
                     /locus_tag="RD477_002963"
     CDS             <1..>426
                     /locus_tag="RD477_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDT7635214.1"
                     /translation="INVSHTTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIK
                     GKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSA
                     FKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          157 a           73 c           80 g          116 t
ORIGIN      
        1 cattaacgtt tctcatacga cgatttatcg ttgggtgcaa gaatatggca aactactcta
       61 tcaaatttgg aaaaagaaaa ataaaaaatc cttttattca tggaaaatgg atgaaacgta
      121 catcaaaatt aaaggaaaat ggcattattt gtatcgagcc atcgatgcag atggtttaac
      181 cttggatatt tggttacgta aaaaacggga cacacaagca gcctatgctt ttcttaagcg
      241 gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca gataaagccc cctctattac
      301 aagtgccttt aagaaactaa aagaatacgg cttttatcaa gggacagaac atcgtaccat
      361 taaatacctg aataatttga ttgaacaaga ccatcgtcca gtaaagagac gcaataaatt
      421 ctatcg
//
LOCUS       DAPBBG010000194          415 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPBBG010000194 DAPBBG010000000
VERSION     DAPBBG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.206"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..339)
                     /locus_tag="RD477_002964"
     CDS             complement(<1..339)
                     /locus_tag="RD477_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7635215.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAPBBG010000195          410 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPBBG010000195 DAPBBG010000000
VERSION     DAPBBG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.207"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>410
                     /locus_tag="RD477_002965"
     CDS             <1..>410
                     /locus_tag="RD477_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7635216.1"
                     /translation="HLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKT
                     EYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYG
                     HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI"
BASE COUNT          152 a           75 c           68 g          115 t
ORIGIN      
        1 gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta
       61 cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg
      121 atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga
      181 gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat
      241 tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat
      301 gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga
      361 agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt
//
LOCUS       DAPBBG010000196          389 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPBBG010000196 DAPBBG010000000
VERSION     DAPBBG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.208"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..374
                     /locus_tag="RD477_002966"
     CDS             <1..374
                     /locus_tag="RD477_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7635217.1"
                     /translation="CDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDL
                     LTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKF
                     LNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          146 a           71 c           59 g          113 t
ORIGIN      
        1 tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg aataccttct
       61 tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag tcactcatag
      121 aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga accatttact
      181 caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt agaaaagagc
      241 agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat acatacaatc
      301 aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca ttagtttcaa
      361 tgtttactga gtaactaatt attttgcag
//
LOCUS       DAPBBG010000197          376 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPBBG010000197 DAPBBG010000000
VERSION     DAPBBG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.209"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(44..>376)
                     /locus_tag="RD477_002967"
     CDS             complement(44..>376)
                     /locus_tag="RD477_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7635218.1"
                     /translation="DRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVK
                     VEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERG
                     SDTSGEAA"
BASE COUNT          102 a           94 c           68 g          112 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtc
//
LOCUS       DAPBBG010000198          370 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPBBG010000198 DAPBBG010000000
VERSION     DAPBBG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.210"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>370
                     /locus_tag="RD477_002968"
     CDS             82..>370
                     /locus_tag="RD477_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDT7635219.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          132 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa
//
LOCUS       DAPBBG010000199          351 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPBBG010000199 DAPBBG010000000
VERSION     DAPBBG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.denovo.211"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            76..>351
                     /locus_tag="RD477_002969"
     CDS             76..>351
                     /locus_tag="RD477_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7635220.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          141 a           59 c           63 g           88 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt c
//
LOCUS       DAPBBG010000200        20862 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.051, whole genome shotgun sequence.
ACCESSION   DAPBBG010000200 DAPBBG010000000
VERSION     DAPBBG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.051"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            267..1304
                     /locus_tag="RD477_002970"
     CDS             267..1304
                     /locus_tag="RD477_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7635221.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="RD477_002971"
     CDS             complement(1393..1716)
                     /locus_tag="RD477_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7635222.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="RD477_002972"
     CDS             complement(1703..2410)
                     /locus_tag="RD477_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7635223.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="RD477_002973"
     CDS             2496..2837
                     /locus_tag="RD477_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDT7635224.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="RD477_002974"
     CDS             complement(3008..3856)
                     /locus_tag="RD477_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635225.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="RD477_002975"
     CDS             4454..5317
                     /locus_tag="RD477_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7635226.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="RD477_002976"
     CDS             5417..5899
                     /locus_tag="RD477_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDT7635227.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="RD477_002977"
     CDS             complement(6162..6629)
                     /locus_tag="RD477_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDT7635228.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="RD477_002978"
     CDS             6752..7603
                     /locus_tag="RD477_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7635229.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="RD477_002979"
     CDS             complement(7854..9812)
                     /locus_tag="RD477_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7635230.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="RD477_002980"
     CDS             complement(9809..10681)
                     /locus_tag="RD477_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7635231.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="RD477_002981"
     CDS             complement(11023..11895)
                     /locus_tag="RD477_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7635232.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="RD477_002982"
     CDS             12023..12592
                     /locus_tag="RD477_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDT7635233.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="RD477_002983"
     CDS             12659..13396
                     /locus_tag="RD477_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7635234.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="RD477_002984"
     CDS             13627..14895
                     /locus_tag="RD477_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7635235.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="RD477_002985"
     CDS             15382..15846
                     /locus_tag="RD477_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7635236.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="RD477_002986"
     CDS             15830..16459
                     /locus_tag="RD477_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7635237.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr(C)"
                     /locus_tag="RD477_002987"
     CDS             16813..18291
                     /gene="msr(C)"
                     /locus_tag="RD477_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDT7635238.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="RD477_002988"
     CDS             18338..18784
                     /locus_tag="RD477_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDT7635239.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="RD477_002989"
     CDS             complement(18983..19498)
                     /locus_tag="RD477_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7635240.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="RD477_002990"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="RD477_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6631 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DAPBBG010000201        16446 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.060, whole genome shotgun sequence.
ACCESSION   DAPBBG010000201 DAPBBG010000000
VERSION     DAPBBG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.060"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..251
                     /locus_tag="RD477_002991"
     CDS             <1..251
                     /locus_tag="RD477_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7635241.1"
                     /translation="LEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDN
                     LIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            486..722
                     /locus_tag="RD477_002992"
     CDS             486..722
                     /locus_tag="RD477_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635242.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            729..2681
                     /locus_tag="RD477_002993"
     CDS             729..2681
                     /locus_tag="RD477_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDT7635243.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2753..3250)
                     /gene="dfrG"
                     /locus_tag="RD477_002994"
     CDS             complement(2753..3250)
                     /gene="dfrG"
                     /locus_tag="RD477_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDT7635244.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3612..4652
                     /locus_tag="RD477_002995"
     CDS             3612..4652
                     /locus_tag="RD477_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7635245.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4750..4938
                     /locus_tag="RD477_002996"
     CDS             4750..4938
                     /locus_tag="RD477_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDT7635246.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4996..5349)
                     /locus_tag="RD477_002997"
     CDS             complement(4996..5349)
                     /locus_tag="RD477_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7635247.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5880..6302
                     /locus_tag="RD477_002998"
     CDS             5880..6302
                     /locus_tag="RD477_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7635248.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6299..6529
                     /locus_tag="RD477_002999"
     CDS             6299..6529
                     /locus_tag="RD477_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7635249.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7027..7227
                     /locus_tag="RD477_003000"
     CDS             7027..7227
                     /locus_tag="RD477_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7635250.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7254..8447
                     /locus_tag="RD477_003001"
     CDS             7254..8447
                     /locus_tag="RD477_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7635251.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8515..10080)
                     /gene="guaA"
                     /locus_tag="RD477_003002"
     CDS             complement(8515..10080)
                     /gene="guaA"
                     /locus_tag="RD477_003002"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDT7635252.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10139..11062)
                     /locus_tag="RD477_003003"
     CDS             complement(10139..11062)
                     /locus_tag="RD477_003003"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDT7635253.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11258..11863
                     /locus_tag="RD477_003004"
     CDS             11258..11863
                     /locus_tag="RD477_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7635254.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12171..13187
                     /locus_tag="RD477_003005"
     CDS             12171..13187
                     /locus_tag="RD477_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7635255.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13328..14629
                     /locus_tag="RD477_003006"
     CDS             13328..14629
                     /locus_tag="RD477_003006"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDT7635256.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14675..15337
                     /gene="deoC"
                     /locus_tag="RD477_003007"
     CDS             14675..15337
                     /gene="deoC"
                     /locus_tag="RD477_003007"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDT7635257.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15358..15756
                     /locus_tag="RD477_003008"
     CDS             15358..15756
                     /locus_tag="RD477_003008"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDT7635258.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15822..>16446
                     /locus_tag="RD477_003009"
     CDS             15822..>16446
                     /locus_tag="RD477_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7635259.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQ"
BASE COUNT         5493 a         2895 c         3246 g         4812 t
ORIGIN      
        1 ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa
       61 gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata
      121 tagggattga taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag
      181 gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc
      241 ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac ctaaatgagt
      301 atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa
      361 tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa ataattattg
      421 taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt
      481 ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat tgggaaatat
      541 cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt actgaaagcg
      601 aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt gaatggattg
      661 ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat agagtgggtt
      721 gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt gtagatgtta
      781 aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat ttattaactc
      841 caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct tttaaatgga
      901 acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt tgtaattttg
      961 ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat aaaatgactg
     1021 gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct attggcagag
     1081 gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt gaagaaatta
     1141 aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa tttcataaag
     1201 aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg
     1261 ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag ttcactaatg
     1321 ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc attcttccaa
     1381 tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat attttaggaa
     1441 ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt ttggagtttt
     1501 tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg tggcttaata
     1561 cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat
     1621 atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct gaggtcttat
     1681 caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg gatgaattaa
     1741 atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt aaaaatgata
     1801 ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa aataggaatg
     1861 cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat ggtttaaatg
     1921 ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg gttaatgccg
     1981 acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt tatagagtat
     2041 tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt aacaagacga
     2101 aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg ctcgattggg
     2161 gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat tatctaactg
     2221 aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga gaaagatatc
     2281 gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa ggattttatt
     2341 cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca aagatgctat
     2401 taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt tatatatcat
     2461 ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt tacgctaact
     2521 tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac aacttttgtc
     2581 gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta gggattacag
     2641 ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag cttggagtaa
     2701 tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc cgttaactca
     2761 ataagttttt tctttcatat acatgaaaat agtagttata cggattttta tcattcttta
     2821 tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga aaaaaagtat
     2881 ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa gggaaaaaca
     2941 aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta cataactcaa
     3001 aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc ttatctctcg
     3061 tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg ttcttcctac
     3121 ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc ttgggaatcc
     3181 tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca gcaatcaaag
     3241 aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc ttatttctat
     3301 caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt tctataagtt
     3361 atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt caattgcatt
     3421 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
     3481 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
     3541 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
     3601 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
     3661 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
     3721 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
     3781 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
     3841 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
     3901 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
     3961 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
     4021 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
     4081 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
     4141 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
     4201 atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg
     4261 ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa
     4321 aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat
     4381 atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac
     4441 tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc
     4501 gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat
     4561 atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata
     4621 aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg
     4681 tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag
     4741 gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga
     4801 ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag
     4861 aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc
     4921 aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta
     4981 tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc
     5041 ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta cctgtaacct
     5101 tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag atacatggag
     5161 taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt tttctatatt
     5221 tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag tgagaccccg
     5281 tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt tatcttcttt
     5341 tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca taaacaggta
     5401 tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat ttattaatgc
     5461 gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca actttataaa
     5521 atttagtcat actcctagaa agtagataaa attggcgact taatggaggg aaaagtcgcc
     5581 atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa aaaacttatc
     5641 cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata gatagaaaaa
     5701 tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca cttaataaat
     5761 atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg gggtttagta
     5821 tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg gtgaaaagca
     5881 tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc tgcaaacaag
     5941 ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca gcaaaagaag
     6001 tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt gatacgtacg
     6061 tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa agtgatttat
     6121 taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta cttcattact
     6181 ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt tcgactgttt
     6241 atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag tgtaaagaat
     6301 gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag atggcgacgt
     6361 ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac gttcattacg
     6421 tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc tacgtggcag
     6481 aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt cccatttttt
     6541 cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt tattccatca
     6601 aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt ctttgacaac
     6661 caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta acacgccatg
     6721 acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt agcaggattg
     6781 ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc cgagcgttag
     6841 aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa attcctgtga
     6901 gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta ttattataaa
     6961 actatttgat ataattatag gtgaatattg gagttttcct agcctataat tgggaaagga
     7021 gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg aacttctttt
     7081 tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca tatctactat
     7141 caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct aggaattcgg
     7201 ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa actatggtta
     7261 aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat ctaggttatg
     7321 attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa aaagaagcaa
     7381 gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag aataaggata
     7441 aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag acacaggtaa
     7501 agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct tattttgata
     7561 aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta aaattatcaa
     7621 agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt gctatggatc
     7681 gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc aatgtgaaaa
     7741 agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta atctctctaa
     7801 tttacaaaga agattactat caacactttt tatttatctc tctgtggttt ttatttatga
     7861 ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat tttgactcag
     7921 gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat agatttgttg
     7981 aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcaca ttaacttatt
     8041 tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta atgagctata
     8101 acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat gctaagctgg
     8161 ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc ttgttaatca
     8221 gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat attgagacga
     8281 ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct aacaaattaa
     8341 atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca aagaatcaat
     8401 ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa
     8461 agataagaag agccagaaac ccttggtata agaggatttc tagctctcct acaactattc
     8521 ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc
     8581 tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa
     8641 gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt
     8701 acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca
     8761 gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg
     8821 tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc
     8881 agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg
     8941 gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg
     9001 cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat
     9061 aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt
     9121 tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc
     9181 tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga ctttgatgat
     9241 gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag
     9301 cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac
     9361 cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac
     9421 ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc
     9481 acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg
     9541 tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat
     9601 cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc
     9661 accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc
     9721 gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc
     9781 gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa
     9841 attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc
     9901 agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct
     9961 caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg
    10021 actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa
    10081 atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct
    10141 aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat
    10201 cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc
    10261 atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat
    10321 aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt
    10381 tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt
    10441 agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag
    10501 gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag
    10561 gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca
    10621 tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat
    10681 ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag
    10741 acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga
    10801 tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga
    10861 acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat
    10921 ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt
    10981 gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt
    11041 agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt
    11101 aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc
    11161 tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata
    11221 gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga
    11281 acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt
    11341 tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt
    11401 cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg
    11461 atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat
    11521 ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat
    11581 cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc
    11641 agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac
    11701 aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca
    11761 aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac
    11821 aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg
    11881 gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt
    11941 tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag
    12001 tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc
    12061 ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt
    12121 gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga
    12181 ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga
    12241 ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg
    12301 aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca
    12361 tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag
    12421 agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag
    12481 aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg
    12541 ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc
    12601 caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg
    12661 aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg
    12721 gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc
    12781 atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga
    12841 ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta
    12901 ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca
    12961 aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca
    13021 tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac
    13081 gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa
    13141 caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat
    13201 tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag taaaccagtt
    13261 tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag acaaggagga
    13321 tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg agctgtcgaa
    13381 agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag attatcaagt
    13441 cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa tcacgaactt
    13501 aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg aaggaataaa
    13561 ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt tagcccccct
    13621 agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag gttacacagg
    13681 tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt cggatgaaga
    13741 atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc
    13801 accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg attctcttcc
    13861 gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt
    13921 ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa gacgtctggc
    13981 aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg ttatctcaga
    14041 tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg aggcaatcga
    14101 aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct taggaagcca
    14161 aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga
    14221 agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa atcaaggggg
    14281 agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca caatggagct
    14341 tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc
    14401 aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg ctgttggatt
    14461 gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat tgacgatcta
    14521 tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata ttgaaattgg
    14581 tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa ggaaaaaagc
    14641 ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca
    14701 tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg aagaagcaaa
    14761 aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca
    14821 attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag gagcaaacac
    14881 acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag atgaagtcga
    14941 tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc aaaaagatat
    15001 cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc
    15061 gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg caggcgctga
    15121 ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg atgtccgttt
    15181 gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta tccataacga
    15241 agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt
    15301 agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga gaagctcatg
    15361 gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa agcctatgtt
    15421 ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa
    15481 ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg cacagctttt
    15541 ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc
    15601 cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct
    15661 gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac cgttcgtgca
    15721 ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca gtcagctgac
    15781 tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa gcaaaattat
    15841 ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc ggcaacaacg
    15901 gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca gcattgatca
    15961 cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa ggactagaaa
    16021 aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat ttccaatctt
    16081 caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga ttcaaaacga
    16141 tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca aacaatacag
    16201 gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt gtatctgcaa
    16261 ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat acgactgaaa
    16321 caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg
    16381 gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc aaagtattga
    16441 atcaat
//
LOCUS       DAPBBG010000202        16182 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.061, whole genome shotgun sequence.
ACCESSION   DAPBBG010000202 DAPBBG010000000
VERSION     DAPBBG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.061"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..835
                     /locus_tag="RD477_003010"
     CDS             <1..835
                     /locus_tag="RD477_003010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7635260.1"
                     /translation="DLLDKPTDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCS
                     DGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQG
                     AAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLG
                     AKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPII
                     AICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILP
                     K"
     gene            1093..2595
                     /gene="eat(A)"
                     /locus_tag="RD477_003011"
     CDS             1093..2595
                     /gene="eat(A)"
                     /locus_tag="RD477_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDT7635261.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2623..3189
                     /locus_tag="RD477_003012"
     CDS             2623..3189
                     /locus_tag="RD477_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7635262.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3400..4461
                     /locus_tag="RD477_003013"
     CDS             3400..4461
                     /locus_tag="RD477_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7635263.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4464..5360
                     /gene="murQ"
                     /locus_tag="RD477_003014"
     CDS             4464..5360
                     /gene="murQ"
                     /locus_tag="RD477_003014"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDT7635264.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5374..7356
                     /locus_tag="RD477_003015"
     CDS             5374..7356
                     /locus_tag="RD477_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7635265.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7421..7867)
                     /gene="nrdI"
                     /locus_tag="RD477_003016"
     CDS             complement(7421..7867)
                     /gene="nrdI"
                     /locus_tag="RD477_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDT7635266.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8078..9502
                     /locus_tag="RD477_003017"
     CDS             8078..9502
                     /locus_tag="RD477_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7635267.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9683..10456)
                     /locus_tag="RD477_003018"
     CDS             complement(9683..10456)
                     /locus_tag="RD477_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7635268.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10453..11358)
                     /locus_tag="RD477_003019"
     CDS             complement(10453..11358)
                     /locus_tag="RD477_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7635269.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11512..11832)
                     /locus_tag="RD477_003020"
     CDS             complement(11512..11832)
                     /locus_tag="RD477_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDT7635270.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12306..12992
                     /locus_tag="RD477_003021"
     CDS             12306..12992
                     /locus_tag="RD477_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDT7635271.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12994..13938
                     /locus_tag="RD477_003022"
     CDS             12994..13938
                     /locus_tag="RD477_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDT7635272.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            14137..14781
                     /gene="pcp"
                     /locus_tag="RD477_003023"
     CDS             14137..14781
                     /gene="pcp"
                     /locus_tag="RD477_003023"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDT7635273.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            14860..16137
                     /locus_tag="RD477_003024"
     CDS             14860..16137
                     /locus_tag="RD477_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635274.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5212 a         2846 c         3489 g         4635 t
ORIGIN      
        1 agatctactt gataaaccaa cggatcaaac tatccgactt gcttccagtt atgccttagg
       61 agaacttatt aaagatgcac tgcttcaaga agtagaacaa attattgtca ctttaggagg
      121 aagcgggtgt agcgatggtg gtctaggatt actccaatcc ttgggtgctt ctttagaagg
      181 gtacacagaa ggaaatcctt tattatctac taaaaaactt cattttgatc gaagaaacgt
      241 tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt accaatccat atacaggtca
      301 acaaggagct gcttggcaat ttggaagaca aaaaggagga atagatacga cgctagcttt
      361 cttagataaa caagcaggaa agattgctca gcaaataaaa gatttctggg aggtggattt
      421 agatgatatt ccaggaagtg gagcagcagg cggtattggt ggagcccttt ttttgttggg
      481 agcgaagatg gtttctggtt tcgatttagt cagccaaatc actggattgg aaaaaaagat
      541 cgaacaagca gatgtcatca ttacaggaga agggcggatc gatcagcaaa cgatccacgg
      601 aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat
      661 ttgcggaagt agagaccaag agctagaagg actttctgat tttctcccaa ttgtcttaag
      721 tattcatttg ggtccaatag gtttagaaga agcaatggaa cacaggcaaa cattagaaaa
      781 aacaaaaatc ttaggacaga cacttagcag atttttcacc atcctcccga agtagttgac
      841 aaaatcattt tctttatgct atcgtaattt caaatatagg acaaagtgag ggatccgtgc
      901 attgaaaaat taagtcgatt gatgaaagct gcattcagac tgtaccttgt ggagacaagg
      961 aagcagtgtg cagattttcg ggattggctt ggttttttcg atgtatgggt ctttttgtgt
     1021 gtatccaacc cttgcatcct ctttagccag ttctgaaaat aggactggtc tttttttacg
     1081 aggtgatgga aaatgtctaa aatcgaaata aaaaatctga cattcggcta cgacagccaa
     1141 ggcacattat tatttgaaca agcaaatcta aattttgaca cacaatggaa actaggactt
     1201 atcggacgaa acggtcgagg aaagacaact ttactgaata ttctacaaaa caaactacct
     1261 tatcaagggc aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat
     1321 aaagaacgtt taacctatta cgtgttaaat gatattacgg attttgagat atgggaaatc
     1381 gaaagagagc tccaattgat gcaaacagat cctgaaatct tatggagaga attcagcaca
     1441 ctatcggggg gagagaagac aaaagtccta ctggcacttt tatttgtgga tgacactcat
     1501 ttcccgttaa tcgatgaacc aacgaatcat ttggatatct ctggtagaaa acaagtagcg
     1561 gcttatttga aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg gggatttatc
     1621 gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc aactggaact ttatcaaggg
     1681 aatttctcta tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat
     1741 gaaaaattga aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa agccgaatgg
     1801 tctcgttccc gagaaggaga taaaacaaag aaacaagtcg gattcatcga tactgaatct
     1861 agacgagtga ataaaggagc agtgggtgct gatgctgcac ggacgatgaa acgatccaaa
     1921 gcaatcgtga atcggatgga gacccagatc agcgagaaag aaaaactatt aaaagatatc
     1981 gaatatatcg attctttgac gatgaatagc caagcgtctc accataagcg acttttaagc
     2041 gtagaagatc ttcaattagg gtatgaaaat ctgttattcg agccaattca ttttacaatc
     2101 gagcctcatc agcgggtggc gatttcaggt cctaacggtg caggaaagtc atccattatc
     2161 cattatcttc tgggggcatt caacggcaag gttataggag aaaaatacca gccaaaacat
     2221 ctgagcatta gttatgcaag ccaaaattat gaagacaatc gaggaacgtt ggcggaattt
     2281 gcagagaaaa accaagtaga ctaccaagca tttttgaaca acctccgaaa gcttgggatg
     2341 gaaagagatg tttttcataa caagatcgag cagatgagta tgggccaacg gaaaaaagtg
     2401 gaattggcta aatctttatc acagccagct gaactatata catgggatga accattgaat
     2461 tatttggatg tcttcaatca agaacaatta gaacaactga tcttgaacgt gaaacctgcc
     2521 atgttactag tggaacatga tcaaaccttc ctggataaag tatctactga gattatttct
     2581 cttgagagaa tctaaccctt gtaatctcaa gaacaacaag gtatggttaa ggggaacttt
     2641 gccaacgaag gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac
     2701 gcacaaggat tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg acagatgcag
     2761 gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat
     2821 tcccctcttc aacgagcaag agatacagca caagcagttg ctgatcgtct cagcttgccg
     2881 ctgaccgttg atgaacgatt ggtagaaatg gattttgggg attatgatgg actgccaagc
     2941 aaagatgaga actttcaaaa agcacgttta gcgttcgctg tacgattccc aaatggggag
     3001 tcagtcttag atgtgtatgc acggatcgtt cctttattga aagaatgcat cgaagacgaa
     3061 gaaaatgtct atctgttagt ttgccataat gccttgataa gagtgatcaa tgcttatttt
     3121 catccgatgc ctaatgaggg gttctttaca ttcatggtag ataatacaga attggtttct
     3181 tatgagtaat tatgtttttg tgttatttaa ataaaatatt tcaaggcact gcttttgctt
     3241 agcagtgcct tttttaggtt atgacaattc ttttgataat ataaactcaa cctttagaaa
     3301 tgaaatttaa ttattttaat aaaattattg aaattttgtt tctttagata tatactctag
     3361 tcatagaaat gaagacggat acagaaaaag agggataata tgtttggctt ttcaatattt
     3421 atgaacgatt ctttgtcaga agagaagaaa cagtatatcc gccaaatggc atccaatggc
     3481 tttgtcggta ttttcacttc catgcatata ccagaagatg atgtttcggc atatcaaaag
     3541 cgattgatcg aactaggaag cgaagcgaaa aagaataaac tagaattgat ggtcgatatt
     3601 tcaggagagg cattggaacg agcgggtttt tcattagaag atctaacgcc tttgaaagca
     3661 attggtgtaa caggtttaag aatggattat catatttcca atcagcagat tgccgactgg
     3721 tcacatcaat tgaaaatcag tttgaatgct agcacgatca cgcctaaaga tatcgatgaa
     3781 ttgaaagaag cagaagctga tttctcacag attgaagctt ggcataatta ttatccacgt
     3841 cccgaaacag ggctggacaa agaatggtat cagaaaaaaa atcaatggct caaaattcaa
     3901 ggtctccttg tccaaggttt tgtaccagga gatacagagt tgagaggccc actatatcaa
     3961 ggattgccaa ctttggaaga acatcgaggg atccatcctc ttgcaggcgc tctagatcta
     4021 ttagcaagta acacggatat tgtttacatc ggagatgcag gtctgtcaga aaacgttcag
     4081 gagcaatttg cttctttcca aaaagaacag acagtcttgc tccatacgga acctgtagat
     4141 gaagaatttt acgaatatat attagggaag catacgaatc ggcaagacga tgcaagagat
     4201 gtcatacgaa gcgtagatgc acgttttcga gagattccgc ctatcccagc aagaaataca
     4261 gctacgcgta tgaaagggtc tattacttta gataacgaaa agtatttgcg ctatatgggt
     4321 gagatccaat taaccaaata tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa
     4381 gttatcaaag aagagctacc attgatcaac caaatcaaag ctggtatgaa ctatcaattt
     4441 ataagaaaag aaggaagata aaaatgagca aaatcaattt agagaaccta acaacggaac
     4501 gcagaaacca agaaactttt ggattagatg agatgagtat ttctcaagcc gttcaactaa
     4561 tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa agaactgaca agtatcgaac
     4621 cagtgattgc taacacgatt gagtccttta aaaaaggtgg acgtctgatt tatatgggag
     4681 ccggaacaag tggaagacta ggtgtgttag atgctgctga atgcgtaccg acattcggtg
     4741 tagaaccgga aatggtcata ggtctaattg ctggtggaga agaagcaatg actgtagctg
     4801 tcgaaggggc cgaagattct tatgagctag ggcaagaaga tctaatcaag ctgaatttga
     4861 caaaaaatga tatagttatt ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg
     4921 gattagatta cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc aataaacaag
     4981 ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca gaatttttga
     5041 caggctctac tcgattgaag tcaggaacag cacaaaaact tatcttgaat atgatttcta
     5101 ctatcagtat gattggtata ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa
     5161 caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt
     5221 acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg gctatcgtca
     5281 tgattttgac aaatagtgat aaagaggaag ctcaagcaaa attagctgaa gcaaacggat
     5341 ttgtaaaagg gaccttatga gggggaaaag accatggcaa aaaacgaact gactttggaa
     5401 gaactggcaa aacaaattta taaaggggca ggcggcatgg gcaatgtaga aagcattgtc
     5461 cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa
     5521 ttaaaagcga ttccaggagt gcttggtgta gtagaagatg aacagcttca agtcatcatc
     5581 ggacctggga aagtgaacaa agtagcacag caaatggttg atttggcagg cgtgaaatta
     5641 ggagaaaaac tttcttctgt aagtacagct acagcaaatt catcagcatc tggaaaagac
     5701 cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct
     5761 aaaatcaaat cggtattaaa agacatttct aatatctttg tgccaatgat tcctgcattt
     5821 gtcggtactg ggatcgtggc agggattgcc gcagttttag caaacttagt cacagccgga
     5881 acacttgatg cagcaacatg gcaacaatat atcgatgtga tgaacatctt gaaaaacggg
     5941 atgtttagct acctagttat ctatacaggt atcaatgctg cacaagtttt tggtgcaaca
     6001 ccaactcttg gtggagttat cggggccgtt gtgatgctga cagggatgaa tccggaagca
     6061 ccactgaaaa acttatttac tggtgaagct cttgcagctg gacaaggtgg gatcctaggt
     6121 gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa aactgcataa agtagtacct
     6181 gatgcggtag atatcatcgt tacaccaact ctttcattga ttgccatcgg tctgattgaa
     6241 attttcttca tcatgccttt agcaggtttc atttctgatg gcatggtcgg tgggatcaac
     6301 tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc taggcacatt attcttgccg
     6361 atggttatgt tcggactgca tcaaattttg actccaatcc atgtgcaaat gattgatgaa
     6421 actggtcgta cgctattgct tccaattctt gcgatggctg gtgcaggaca agtgggtgca
     6481 gcattggcat tatggattcg ctgcaaaaaa gataaagaat taacagaaat gatcaaagga
     6541 gctttaccag tcgggatcct aggaatcggc gaaccattga tttacggagt aacattacct
     6601 ctaggacgcc catttattac tgcttgtatc ggtggaggta tcggcggggc agtgatcgga
     6661 atgatcggaa atgtcggggc tatcgctatt ggaccttcag gagccgcttt gatcccattg
     6721 atctctgacg gtaaatggtt aggctacatc cttggattat tggcagctta tgctggtggc
     6781 ttcgttgcta cattcttctt cggtattcct aaagaccgtt tagaaaaaga agaattagcg
     6841 actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa
     6901 agtgtgacta tcctctcatc agttgctgac ggaacagttg tttctttaga taaagcaagt
     6961 gatccagttt ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc agaaaacgga
     7021 catatctatt ctccagttac tggggtagtc agctctgtct tcccaacgaa acacgctatt
     7081 gggatcacaa cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa cacagttgac
     7141 ttaggcggta cgccatttga tgtgaaagta acagaagggc agcaagtgac acctgatacg
     7201 cttgtagcag aagctgactt agaagcgatc aaagcagcag gcaaagaaac atccatgatc
     7261 gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca
     7321 aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa
     7381 agaagaaact gaaatctatt tttcagcttc ttctttttta ttatttattg ttttctgcta
     7441 aaatctgata aattcgtgtc acatcagatg gtgttccccg taattcataa tctgccaaga
     7501 aaggaaagcc aaatgtttgt gcatattgtt tggctgttag acaatactga ttattgaagt
     7561 ttttatttcc gctaccgact acaccaagac aaagtttatg attgtcttca tgttccagat
     7621 attcacgcat cgtttcggtc aagatttctg tatcaccgtt atctaaacca tttccgccgt
     7681 ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt
     7741 catggatttc ttttaaagta acttcaggat tgacaggatc ttctgcatgc attttttcag
     7801 catattctgc tagatgtttg gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa
     7861 tgttcatgga tcatcctcca attctttcct tttagtatag catctttttt aagttttttg
     7921 gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa aagccggtag
     7981 aattatattt ctattaaagt ttttattaat taagtaaaaa taaaaaaggt ttgttatact
     8041 ttttgataaa agcggtttta ttttggagga tgcttacatg gatacagagc aacagaaaaa
     8101 aggaattggt ttaattccac ttgctgcctt agtaattggt tcagcgattg gaggtggcgt
     8161 atttggtatt atgacggaca tctccggatc ggctggcggc ccagctattt tcagttgggt
     8221 actcgtagga acagcaatgt tgatgctctc cttgtcgatc aataatatca ataaaaaacg
     8281 tccagattta aaaggcggga tctttagtta cgcagaagaa ggttttggcc gtttcgctgg
     8341 ttttatcagc ggttggggtt actggctcac atgctggtta ggcaacgtag cttttgcaac
     8401 attgttgatg agcgcattag gatatttctt cccagtattt gaaggtgggc aaaatctggc
     8461 atcgatcgtg atgagtacag tctttttatg gggatatgca tggctagtga atcggggagt
     8521 cgaaaatgct agtttcgtca atgctatcgt cacattctgt aaattagtgc cattattttt
     8581 atttattgta gtagctatca gcacgttttc gatccaacaa ttcattgata acttttctag
     8641 cgacattata ttgaatgcga atggacagcc gatcccgttt tccagtcaag tattgagttg
     8701 catcatggtg atgctttggg tatttgttgg tattgaagga gcctctgtcg taggttcacg
     8761 agcaaaaaaa caatcagatg ttgggaaagc aacgattcta gggatattcg gcttgattat
     8821 tatttacgtc ttggtttcca tgcttccata tgggacaatg acgatgaatc agctgcaaac
     8881 gatcaaggcg cagcctgcta tgggctatgt gtttgaaatg atggttggta aatggggagc
     8941 agtcgtgatc aacggcggtt tgatcatttc tttagttggt gtttggctct catggacgat
     9001 cttaccgatt gaaacaatgg gaaacatggc tgaagacgga cttttgccta aagcatgggg
     9061 aaaactcaac aaaaatgggg caccaactta tgctatcgta ttgacgacgt tatgtacgaa
     9121 tgtcttttta ctttcattat tattcactga cagtgcttat aactttgctt acacacttgg
     9181 tacagcagct attttcttca cgtggctttt cttaggattg taccaaatga aactttctta
     9241 tcagcggaaa gaatgggtcc aatttttagt agggctttta gcaagtgcct ttcaagtatg
     9301 ggctatgcta gtcgtagcct ttaatgaagt aatgcttgtt cttgttcttt tcttgccagg
     9361 catttatttt tacctcaaag cacgtaagga gcaaaacgag cctgtacaac atatccactt
     9421 agataagatt ttacttatag gtgtgacgat ggtcggagtg attgcaatgg ttttatttgc
     9481 cactgggatg cttcacgtat agaagtataa aagaagtata aagagaggtt gtgagcctgt
     9541 cgtttattca aaaacatact aggaaaggag ctgtgacaca aatgttgtca cagctccttt
     9601 ttcagaataa acggtgttta agagacagcc aatcggcaat aaggctgaac gtctctttca
     9661 cagcctcttt tttgatgagt catcatacat tttttcgctt aaatacgaga tatcctaatg
     9721 ctaagaacaa aacgatgtaa gctaaattac cgaatagcat ttgattagta gaaagatgag
     9781 tcaattgctt catgatctct tcgtttccaa tttggttttg cagattcaac atactgatcg
     9841 gattccattt gatccactcc catttttgga tgagtgcaat aaggatacca gatatgattg
     9901 acgaagcaaa ataaaagacg attcctgctg agatggatgc gccggaacta ttgataaagc
     9961 aagcaatcat caacactaaa cttaaaatca gccataaacc gatataacta cccaaagtat
    10021 aagtgaacaa gctttggatc agtgtttgac cagtagaaac cttctccgtg aaggaaatcg
    10081 aagggaaaag aatcagtttc atcaacacag tgacaataat agctaaaagg tataaataaa
    10141 cagaataaat aactaatgtg atccatttgc ttactaatat ttctcctcga gaatactttc
    10201 gataaaggag atttttcaat gtaccatatt gtgcttccat cgaaatgatc gtgcttgcag
    10261 ctgcaatcat gataaagaca atccaagatg tggcactaaa aagctgagca gtcatttcaa
    10321 ctggatcaat gatcgtcgtg tattttttgg ctagaagtgc tgttcctatg agcaaaacaa
    10381 ctaatagaac agataaaaca gcagttgact ttttcttgat caatttaaat aattcttgtt
    10441 ttacaagcaa tgtcattttt cttcttcccc ctttactgaa tccagaattt ccaatagaga
    10501 agattccaaa tcattcgtca gatgctggac gtctgtcaca tggatatttt gtgcagacaa
    10561 tagctggatc acggttccta ataattcttc gttttctttt aaaagcttga ttttatcagg
    10621 atcttccaaa gtatatccag cttctgtcaa gatcgttcga gcagctagat catttgttgt
    10681 agagataacg atgaaatctt gcgctgtttg gttcaattca gccattgtac attgtttgat
    10741 gatctttcct tgattgatca ggatcagctc atcgaccatt ttttcaagct cgctgagtaa
    10801 gtgactggag atcaagaaag aggtgccctg agtagccaag tcggtaatga tttttcgcac
    10861 atctcttgtt gcttgtggat ccaaaccgtt catcggctca tctaaaataa cgatagacgg
    10921 atggttcagt aaggcaagag caattcccaa tttttgtttc attcccaacg agtaggattt
    10981 cgcttttttg tgaatgtact tttccatctt gagctgattc acgatagtgt cgatagcaga
    11041 tacatcgttg gtttcagaga ataacttcag atgatcatag cctgttaaga atggatagat
    11101 acccgggtat tcgatcaatg ctcctacttg ttccaaccct ttatgactag agggagaaat
    11161 ctcattttca ccaatacaga ttgttccatc tgagtaatgg atcagtccta aaatagcttt
    11221 catgatcgtt gtttttccag cgccattagg acctactaat ccaacgattt tgccagggga
    11281 taagctaaaa ctgacatcgc tgagggcttg tttgtgacca aagttcttgg ataattcttg
    11341 gaccgaaaga cttttcaaaa atattcactc cttttctata gatagtacaa gcatacacct
    11401 gatgagtgga gaagtcgcta ggtcattgga cttatttctt attttttcat aaaaaaattg
    11461 aatattaaat aaaaaggctt attttttctg atcagtactt attccaagtg tctagtggcc
    11521 gctagtaatc ttgatgccaa tgatactgat gagtagcatc cccacaaaga accaagtgga
    11581 aagattcagc ttatcatgaa acaagaccac accaattatg accgaaccaa ctgcgccaat
    11641 ccctgtccag ataggataag agagactcat cggcaaactt ttgattgcct ttgacaaaaa
    11701 gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa taattgattt tagaaaaacc
    11761 atcactccat ttcatcatcg ttgaccagaa tacttccaaa attcctgcta tgattaattc
    11821 catccatgcc atacttacat tcctccttaa ctataaaaaa atacacaaaa agaggaaaat
    11881 catcccttca aagacctaat ggaatgatca tcctctttat cctacccggt ggcagattgt
    11941 atatcttttt ctgtagtata gcacacctat ccagatatcg gcaatctcat actttttagt
    12001 aaagtgtacc aagcactgac ggatatatcg ttagcggaaa agaagatgaa tgctttgcaa
    12061 tttcatagaa agaagttgtt atttattaat catttaaaaa aaacagtaac tagaccttat
    12121 ttctatatat tatcgggcaa taaattaaaa aaagctttac cttacaataa gtaagataat
    12181 ttttgtgata aaaaaataac gaaaaatatt taaaaatagt gtctatttcg tgagaaaaca
    12241 actatatttt ttatgtgttt tgctttataa ttaaaaaaat attgtcattt tatggaggaa
    12301 aatatatgga aaattatctt agccttttag gcgtcttgat tgttattctc gggtttgcat
    12361 ttaaattaga ctcgatattg atcgtaatgg ttgctttagt tgttacagct ttgactggcg
    12421 gtcttggcat tcaaggaatg ttagaacttt taggtacaag ttttgtaaac aatcgcggga
    12481 tggcgatttt tatcatcatt atgttggcaa caggaacgct tgaaagaaat ggtctgaaag
    12541 aatcagcggc ttccttgatc aagcgattca agaaagtatc tgctggtatg attgtagata
    12601 tttatgggat ctttcgaatg atctttgcgg cttttaacgt gagttttggt ggagttgctg
    12661 gatttgttcg tccaatcatt ttaccgatgg cattggggac catcgaatca aaagggttag
    12721 aagtccatcc taagcatgaa gaagaactaa aaggtatggc ttcagctatg gaaaatgtct
    12781 gctggttctt tggccaagta ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa
    12841 cactgaaaga tctaggatat gacgtaacat tgggacagct tgctttagtg gaagttccag
    12901 tggctatcac ggctttagtt gtggcaagtg tctacttcta tcttaaagaa aagaaattag
    12961 cgaagaaata ttatccagga aaggggcaat aagatggact tttttacaaa tcctgatatt
    13021 ttattaggcg ataaattact ggagatcctt tatatcgtga tgggactcgt attgatttac
    13081 actggagtaa gaaatttaac agataaaacc aatcctcatc gttatgggtc agcctttttc
    13141 tggacggtct taggggtagt tattgcagga ggaagatggc ttccggcaat cgtgaacggt
    13201 ggcttgatct ttgcgatgac cataccagca attgctaaga aagtcagcaa aggagaaagc
    13261 cggcttccat caaaagaata tatggaaaaa atgtcagaca aactaggaat gaaaattttt
    13321 attccagctt tgagtatcgg tgtctttgcg atcttgtttg ctctatttac caatttaggt
    13381 gctttagtcg gcgtgggcgt cggtgttttt gcagccatgc taatcttgat gttcttttct
    13441 cgagataatc gaccaagtac atttttagat gatgcagcag atatgctggg cacagtaggg
    13501 ccgttaagca tgttgccaat gcttttagct tctctaggtg cggtatttac aagcgctgga
    13561 gttgggacag tgatttccaa tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc
    13621 ggcatcatca tttacgctct tggcatggtg atatttacgg taatcatggg gaatgcattt
    13681 gcggccatca ccgtgatgac ggttgggatc ggtgcaccat tcgtatttag tcatggagcg
    13741 aatccagcgt taataggaat ggttgcattg acttgcggtt tttgtgggac cctgctgaca
    13801 ccgatggctg ctaacttcaa tatcgtgcct gtagcgatgc tggagatgaa agacaaatac
    13861 ggagtgatca aaaaccagtt gtttattgcg ttatttatgc tagttttcca aattgcttat
    13921 atgattctat ttaaataata gaagagaggg gttgtgacag aataattgtc gcaacctctt
    13981 ctaacattgt agtggaacac tattttttta taagaatata ttaagtttga tctgttatgt
    14041 tatttaaact attgtttcct ttatttacgc tgttcaagga aaatggtatg ataaaagata
    14101 accatatgtt aaatcaatcg gacggaggaa caacagatga aagtactagt aacaggtttt
    14161 gatccatttg gtggggacaa agtgaatcca gcctatgaag cagtaaaaaa aatgccagat
    14221 gaaatcagcg gtgctgaaat catcaaagtg gaagtaccga ccgtattcga aaaaagcagt
    14281 caagtagtca aagaagccat ccaacagcac caaccagatg tcgtgatctg tgtcggacaa
    14341 gcaggtgggc ggtcagcagt ctcgttcgaa cgtgtcgcaa tcaatctagc agaagcaaga
    14401 atccctgata atgaaggaaa tcagccattc gatacggctt tagaagaaaa cggtccagcc
    14461 gcttacttta cctcattgcc aatcaaagca atgacaaaaa atgtacaaga tcatggtcta
    14521 ccagcgtaca tctcttatac agcaggaaca tttgtctgca atgatatcat gtatcgtttg
    14581 cttcatttga tcgaaacaca attcccggca attcgaggtg ggttcatcca cgtgccattc
    14641 tcacccgatc aagtgatcga tcgtccagtg ggtacgcctt ctatgtcttt agaagacatt
    14701 gcttcttctt tgacttatgc agttgaagca gcagtagaaa ataaagaaga tatttccgga
    14761 aatgccggaa caactcacta atttagcaag aaacactggc tggttgctaa cgttatgtta
    14821 gtagccagct ttttgtaatt agaaaggaaa gaaacagccg tggaagaatg gcaatccgtc
    14881 tttgaagaat ggtttccaaa agaaatcagc aaaagctatc ccatcaaaat ttctaaacaa
    14941 tacacaagca gtcaacgttg ggagatctat gcaaaattaa cgaaaaaaca gcgggaacta
    15001 gtcgataagc accgccgata tctaatcagc tctcgtttta tggaagaaca ttatttagct
    15061 gcaacagatt gggtgttttc tgacttcaag atcaatccgt tttttcgaac aaaacgaagc
    15121 cagcaaaaat tgtattgtga gtgtggtaga gaattaaaag tgcaatacat cgttaaatcg
    15181 ccaaaaaccg gaaagatcct aaaactaggc atcaaccact ttgccgatca cctgcatgtc
    15241 tctccgacag tggcagcttc gatccatcag ggaatgacaa aagttgatct ggccttagac
    15301 gaattattat ggcttaagca gaagaatatc gattttcctg aaggactatg gcaaaaatac
    15361 tgttttgtct tgtaccaaaa tcgtcggatg aaacagccat atttaccgga tataaaatta
    15421 gctcagcggc tcgcagaatt tcgccaagta gaaatgccga tttacatagc ggattatcaa
    15481 gcattggaaa atgaaatcaa gaaaataagt gaacacatca atgggcagtc aaaaaagcga
    15541 cagataaaaa aagaactatt tgacgacttt gcagaagaat tagtaaagga tgtagaagaa
    15601 ttcttgatca actatcgcgc atttttacgt aaagattggc aatccatcgt atatgaagaa
    15661 gtgccagtac atcctaatgc ttattttgaa acatttattt ccgtgttgag aaagacaaaa
    15721 cgtcaacgaa caccagaagt cactgctcag atggaatatt ttgcgaaaaa ccaacgattc
    15781 atccaaccaa aaatctatct atttatttgg aagcaatatt gtcgctatgg ttttacagaa
    15841 gggttcttcg acagcattcc tcggatagtc agaaatggtt ttttaaaagt attgcgtaaa
    15901 gaacgagaag ctatacaatc tgctgataag aaagaccgta cagtctcaaa agaaaaatgg
    15961 caactagtag tgaaagatat ccaatctgga aatgtgcaag agacaatcga caaatggaaa
    16021 ggaaaacatt atcgttttac tgaagctcag aaacaagcgt tggaatatta tcagaagtta
    16081 gaagaatcac tgcgtttcaa tgatgaggca agaaagtatc tgaaggaatt actataaaaa
    16141 atagaggctg ggacagaagc gtttaactcc aagaaataag aa
//
LOCUS       DAPBBG010000203         7959 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.090, whole genome shotgun sequence.
ACCESSION   DAPBBG010000203 DAPBBG010000000
VERSION     DAPBBG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.090"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            128..1564
                     /locus_tag="RD477_003025"
     CDS             128..1564
                     /locus_tag="RD477_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7635275.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="RD477_003026"
     CDS             1753..4200
                     /locus_tag="RD477_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDT7635276.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="RD477_003027"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="RD477_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDT7635277.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="RD477_003028"
     CDS             complement(5690..6793)
                     /locus_tag="RD477_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7635278.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD477_003029"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD477_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDT7635279.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAPBBG010000204         5422 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.104, whole genome shotgun sequence.
ACCESSION   DAPBBG010000204 DAPBBG010000000
VERSION     DAPBBG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.104"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(39..2657)
                     /locus_tag="RD477_003030"
                     /pseudo
     CDS             complement(39..2657)
                     /locus_tag="RD477_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2803..3378
                     /locus_tag="RD477_003031"
     CDS             2803..3378
                     /locus_tag="RD477_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7635280.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3592..4287
                     /gene="vanR"
                     /locus_tag="RD477_003032"
     CDS             3592..4287
                     /gene="vanR"
                     /locus_tag="RD477_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDT7635281.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4265..5416
                     /locus_tag="RD477_003033"
     CDS             4265..5416
                     /locus_tag="RD477_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7635282.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCCKVLNKE"
BASE COUNT         1693 a         1022 c         1031 g         1676 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaacctt catccgatat ataattcaaa
       61 ataaagatcg ttttttctat tcggcccatc tcacgtaagg ctgtagctaa gctgttttgt
      121 cttgaatagg aacctagctt ccccataata agggatgctg aaactgttcc ctcccttata
      181 gaatgagcta atcgcaaaac atcctcataa ttttctttaa tgacctttgt atttatttgt
      241 ccacgtaaaa tggcttctag ttttggatac tcacttgctt tatctatcgt aaataatttt
      301 gagtccgata aatcccttat tcttggggca aatttaaatc ctaataaatg agtcagtccg
      361 aatatttggt cagtgtaacc ggcagtgtct gtataatgtt cctctatgtt tagatccgtc
      421 tcatgatgta acaaaccatc caaaacatga atcgcatctc ttgaattagt atgaataatc
      481 tttgtgtagt aagaagagaa ttgatcactt gtaaatcggt agatggtggc tccttttcca
      541 gttccataat gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata
      601 ccatctgacg aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga
      661 aagtttacta atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat
      721 acattggcta gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca
      781 atattcattc ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt
      841 tttcgattat tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc
      901 atgagtaaat cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt
      961 tttgcttctt ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga
     1021 gaaaccccat ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg
     1081 ctggttctct ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc
     1141 ttcgattgat tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg
     1201 ctgccaacaa tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc
     1261 atttcatagt aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat
     1321 cgttttgaaa taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc
     1381 attcctcgga taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat
     1441 tccaatactc ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt
     1501 aagtctaaat aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag
     1561 acaaaggtat tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct
     1621 cttgctttaa ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt
     1681 ttaccgtttt gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt
     1741 atttgcctat catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa
     1801 tagatggtta atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg
     1861 tatcttgagc ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc
     1921 actgtttcta aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt
     1981 tcgggtgaag gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg
     2041 gatggatgct gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat
     2101 ttactaaccg tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt
     2161 tcaagtgtag tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca
     2221 tgcagtagat gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc
     2281 attcgatatt cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa
     2341 tgatcccaaa gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca
     2401 atctgtttcg atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc
     2461 caaccgggat accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc
     2521 cttcgcttat taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc
     2581 cattcatctt cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta
     2641 cctctcgcaa ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat
     2701 tatatatcaa tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt
     2761 aagagcgtct cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat
     2821 attcgtgtca gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc
     2881 ggaatggata ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt
     2941 caaaaagtgt tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga
     3001 atcactcgta gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca
     3061 agtttaaaat cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa
     3121 ttcttaatta ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga
     3181 caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag
     3241 tatcataaaa atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg
     3301 actgtaaatc aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta
     3361 tcagaagtga ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa
     3421 aaggaaacta taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa
     3481 agaatcatca tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc
     3541 ctataatctg ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat
     3601 aaaatactta ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa
     3661 aacgagaatt atacggtttt caaatactat accgccaaag aagcattgga atgtatagac
     3721 aagtctgaga ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact
     3781 atctgtcaaa aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat
     3841 acagaggtag ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc
     3901 tttcgcccac tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc
     3961 agtggagtaa aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt
     4021 aacacccatg agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca
     4081 atactgcgaa tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat
     4141 gagatatggg gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg
     4201 catttgcgcg aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg
     4261 ggggttggtt ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact
     4321 ttacatgtat atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc
     4381 aatgatccga gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt
     4441 aaatcacctg gacgcgatga aattatatca atattccata cggaacaata tagatatctt
     4501 tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt
     4561 cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa
     4621 acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg
     4681 gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt
     4741 tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct
     4801 gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt
     4861 ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa
     4921 cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat
     4981 gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc
     5041 cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt
     5101 gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc
     5161 cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc
     5221 tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc
     5281 gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc
     5341 ggaaagcaat gataactata cgacgtttag ggtagagctt ccagggttct gttgcaaagt
     5401 tttaaataaa gaataaaatc cc
//
LOCUS       DAPBBG010000205         5183 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.108, whole genome shotgun sequence.
ACCESSION   DAPBBG010000205 DAPBBG010000000
VERSION     DAPBBG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.108"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            74..190
                     /locus_tag="RD477_003034"
     CDS             74..190
                     /locus_tag="RD477_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDT7635283.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            206..2125
                     /gene="tet(M)"
                     /locus_tag="RD477_003035"
     CDS             206..2125
                     /gene="tet(M)"
                     /locus_tag="RD477_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDT7635284.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2235..2285
                     /locus_tag="RD477_003036"
                     /pseudo
     CDS             2235..2285
                     /locus_tag="RD477_003036"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2319..3695
                     /gene="tet(L)"
                     /locus_tag="RD477_003037"
     CDS             2319..3695
                     /gene="tet(L)"
                     /locus_tag="RD477_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7635285.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            4290..4601
                     /locus_tag="RD477_003038"
                     /pseudo
     CDS             4290..4601
                     /locus_tag="RD477_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            4736..5143
                     /locus_tag="RD477_003039"
                     /pseudo
     CDS             4736..5143
                     /locus_tag="RD477_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
BASE COUNT         1745 a          734 c         1008 g         1696 t
ORIGIN      
        1 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
       61 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
      121 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
      181 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
      241 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
      301 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
      361 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
      421 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
      481 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
      541 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
      601 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctatggaaat
      661 tataatcaaa cagaaagtag agctgcaccc taatatgtgt gtgatgagct gtacggaacc
      721 tgagcaatgg gatgtggtaa tagaaggaaa tgatgacctt ttagagaaat atatgtccgg
      781 taaatcatta gaagcattag aactcgaaca agaggaaatc agaagatttc agaattgctc
      841 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
      901 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
      961 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     1021 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     1081 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     1141 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     1201 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     1261 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     1321 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     1381 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     1441 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     1501 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     1561 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     1621 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     1681 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     1741 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     1801 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     1861 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     1921 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     1981 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     2041 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     2101 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     2161 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     2221 ttatgaagtg aaatgaatgt aacagggttc aattaaaaga gggaagcgta tcattaaccc
     2281 tataaactac gtctgccctc attattggag ggtgaaatgt gaatacatcc tattcacaat
     2341 cgaatttacg acacaaccaa attttaattt ggctttgcat tttatctttt tttagcgtat
     2401 taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt aataaaccac
     2461 ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt ggaacagctg
     2521 tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt ggaattataa
     2581 taaattgttt cgggtcggta attgggtttg ttggccattc tttcttttcc ttacttatta
     2641 tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta atggttgtag
     2701 ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt ggatcgatag
     2761 tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat tatattcatt
     2821 ggtcctatct tctactcatt cctatgataa caattatcac tgttccgttt cttatgaaat
     2881 tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt atactaatgt
     2941 ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct tttcttatcg
     3001 ttagcgtgct gtcattcctg atatttgtaa aacatatcag gaaagtaaca gatccttttg
     3061 ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt gggggaatta
     3121 tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa gatgttcacc
     3181 agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg agtgtcatta
     3241 ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac gtgttaaaca
     3301 tcggagttac atttctttct gttagctttt taactgcttc ctttctttta gaaacaacat
     3361 catggttcat gacaattata atcgtatttg ttttaggtgg gctttcgttc accaaaacag
     3421 ttatatcaac aattgtttca agtagcttga aacagcagga agctggtgct ggaatgagtt
     3481 tgcttaactt taccagcttt ttatcagagg gaacaggtat tgcaattgta ggtggtttat
     3541 tatccatacc cttacttgat caaaggttgt tacctatgga agttgatcag tcaacttatc
     3601 tgtatagtaa tttgttatta cttttttcag gaatcattgt cattagttgg ctggttacct
     3661 tgaatgtata taaacattct caaagggatt tctaaatcgt taagggatca actttgggag
     3721 agagttcaaa attgatcctt tttttataac aggaattcaa atctttttgt tccattaaag
     3781 ggcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt attttgagtg
     3841 gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt gctgagtctg
     3901 gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt aaaggtggag
     3961 attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt taaacgagca
     4021 cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt ttcttttttc
     4081 tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg gtcggcactg ccgacagcct
     4141 cgcagagccc acctttacga agtaaagtat agtgtgttat actttacatg gaagttgtac
     4201 cgaaatattg tgttgtgaag tctatataat tttaaaatta tgaagtctat atacgtgcgt
     4261 aaagatattt aaagagatta aaaataataa aaataaaaat tgaattaaaa agagatatgg
     4321 ttgattattg gaacgattgt tttaatgatt tacatatatt acaaccagat tggaaaacta
     4381 tcgaacgtac ttctgataga gcaatggttt ttatgttgtt gaatgatgaa gaagaatggg
     4441 gaaaattaga aagacgtact aagaataaat ataaaaaatt aattaaagaa atatctctaa
     4501 ttgatttaac tgatttaatg aaatcgactt taaaagcgaa cgaaaaacaa ttgcaaaagc
     4561 agattgattt ttggcaacgt gaatttagat tttggaagta aagtaacttt gttttataca
     4621 aaatgttact ttagtataat taacgtttaa gaggtgacac aatatatgaa aaaatcagag
     4681 gattattcct cctaaataca aagagataag atgaaattta ggaggaaaat tatatatgac
     4741 ttttaatatt attaatttgg aaacttggga tagaaaagaa tattttaatc attatttcaa
     4801 tcaacaaaca acttacagtg ttactaaaga atttgatatc actttactta aaagtatgat
     4861 aaaaaataaa ggatatgaac tgtatcctgc tttgatttat acaattgtaa atattataaa
     4921 tcaaaataaa gtatttagaa caggaattaa tagtgaggga aatttgggtt attgggaaaa
     4981 attaaaccct ttatatacag tctttaataa agaaactgaa aaattttcta acatttggac
     5041 agaatcaaat gttagtttta attcttttta taatagttat aagagtgact tacttgaata
     5101 taaagataaa aatgaaatgt ttcctaaaaa accaatacct gaaaaggttc tgttgcaaag
     5161 ttttaaataa agaataaaat ccc
//
LOCUS       DAPBBG010000206         3715 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.126, whole genome shotgun sequence.
ACCESSION   DAPBBG010000206 DAPBBG010000000
VERSION     DAPBBG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.126"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            389..472
                     /locus_tag="RD477_003040"
     CDS             389..472
                     /locus_tag="RD477_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDT7635286.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            597..1334
                     /gene="erm(B)"
                     /locus_tag="RD477_003041"
     CDS             597..1334
                     /gene="erm(B)"
                     /locus_tag="RD477_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDT7635287.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1597..2460)
                     /locus_tag="RD477_003042"
     CDS             complement(1597..2460)
                     /locus_tag="RD477_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7635288.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNLIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(2462..2734)
                     /locus_tag="RD477_003043"
     CDS             complement(2462..2734)
                     /locus_tag="RD477_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7635289.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(2751..2960)
                     /locus_tag="RD477_003044"
     CDS             complement(2751..2960)
                     /locus_tag="RD477_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDT7635290.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(3030..3629)
                     /locus_tag="RD477_003045"
     CDS             complement(3030..3629)
                     /locus_tag="RD477_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7635291.1"
                     /translation="MKNGNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRL
                     LATLLAPLKSDYDLIIIDTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYL
                     IDLQEQFNPGLDMIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVST
                     WSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1145 a          624 c          603 g         1343 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc ttttgtacat ttaacttgac
       61 ggtgacatct ctctattgtg agttattagt ggtacagttt tcaaccgttt taattataaa
      121 aaagtggtgc atttttaaat tggcacaaac aggtaacggt tattgcaggt gtatttctta
      181 tctatgggtt taacatggat tttatcatta aaatcatgag tattgtccga gagtgattgg
      241 tcttgcgtat ggttaaccct aaagttatgg aaataagact tagaagcaaa cttaagagtg
      301 tgttgatagt gcattaaaaa ttttgtataa taggaattga agttaaatta gatgctaaaa
      361 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgtt atgtagataa
      421 aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta
      481 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa
      541 caaatcgttt aacttctgta tttgtttata gatgtaatca cttcaggagt gattacatga
      601 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
      661 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
      721 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
      781 atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc
      841 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggagtattc
      901 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat gcgtctgaca
      961 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag
     1021 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct
     1081 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg
     1141 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat
     1201 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca
     1261 atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta
     1321 acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa
     1381 gggaatgtag ataaattatt aggtataggg cacctctaat aactaccaat taattcacct
     1441 caaaatgaaa ttagaaaaaa acagcgaaat ttttaaatct atttcttatc gatacaaatt
     1501 cctcgtaggc gctcgggacc ccaacatgag gaaatgtttt tataaaaaaa gagtagttac
     1561 cgttttttgg taactactct tttccttttt taaggtttaa atacctggaa gtttaggtgt
     1621 tttgggtatt ggtggtgtag gtggctgcaa gctttccaat ttttgttgaa ttgctttaaa
     1681 ttcaggtgtc tcttggtgtt tatttagaac cattttttgc tctattcgtt ctaaagtcgg
     1741 ttgaatttct ttccctgata ctttacgatt caattctttt tctaaggttt cttttggact
     1801 aatagaaggt gtttccaaac ttgaatagag ttttactcct tctctgttat atagccttat
     1861 atcgctaaat aagcccgttt tatgaagggt ttctaaattg gtcggtaagt ttttgacaac
     1921 aatatcatgc gcttgttttg gtgttgccct ggctgtcatt ggatcatctg catacatggt
     1981 ttcatatcgt tcaattgttc ctaaatatga gttgatttta ggtactgcca tgacatacat
     2041 ttttgtttca taacctttgg cttgaagcat tgttgcggtt tgaataggaa cgtctgttgt
     2101 tcgtcctgta ccttcgatca ccaaattata cccttgatcg ctcaaacggc ttatgatcgc
     2161 ttctgtcatg cgattagaat aaggggtaac gtgttttact acgtcttttt cataaagttt
     2221 cactagttca tcaaaattag ggtgctgttg tttaaaggta tcattatcaa tgacaataag
     2281 attcccttgt gtttcttcaa aaattgctga tcgcaaactg gttttccctg accctggttg
     2341 cccaccaagt aaaaaagcgg ttggcgattc aaccgctttt tttccttgaa tcaattcttc
     2401 taaattatca tttaagcgat tctcaaattg tttgtcagta aaattgacta tatttgccat
     2461 attaagccac tttctcttta ttcaaaactt gttttgagta acgattcatt gaccgccaat
     2521 actcatgaac ggcttgtaat tcttctaaag aataatcaaa aagatttaca cgttttgcaa
     2581 gctttaattg gtttaataaa ttgacttcca ataattgaga gtcattttta tttaattcat
     2641 ggttcaaaac atagtttaat actcgattat aaacgcttgc cgataattcg ttaatgatct
     2701 ctatttcaaa tgttttttca tacgtaactg ccatttcatt ttcactcctt ttaaagtttg
     2761 tcaggtaaat atttccgtat atattcttct aaggcttgat ccataacttc ttttacgttt
     2821 ccgccattct ttgctgtttc gatttttatg atatggtgca agtcagcacg aacacgaacc
     2881 gtcttatctc ccattatatc tttttttgca ctgattggtg tatcatttcg ttttgctttt
     2941 tgtgcgccta aatttcccac aatcactcac ttctttctat ttcttcttac tcttatttta
     3001 tcatcaacaa tcacttgtga tttgtgattc tattcttttt cgttttctaa ttgaataatt
     3061 cgctcaagca tttcaaaaaa tacgttctta tacatggata aaactttttt gtcatagcct
     3121 ttatgctctg taatgccgtt tttagaccag gtacttactt tattacttcg cttgataata
     3181 ttttggaaaa ccaagttatc ttctttatgt tgcttataca gttcttccag gtttgatttt
     3241 atcgttgcgc tgtccgtatc aactaaataa ggaacaaaac cgatcatatc tagtccaggg
     3301 ttaaattgtt cttgtaaatc aatcaaatag gaaatatagt tttgaatgtt gtttgtactt
     3361 tcttcttctg cttgtaaagg gatcataacg taatcactcg ccacgattgc attatttgta
     3421 taaacgcttg gcgttggtac agtatcaata ataatgagat catagtcact ttttaaaggt
     3481 gctaaaagag tagcaagcaa tctactttcg ttttcaaacg tccatgagcg agttaatttt
     3541 ggcagtaaca tcaaatcaaa cgtgccagga atcaagtcta aattatcagt caaatgaata
     3601 atagaagaag ccaagtttcc atttttcagt ccttcataaa aattgacacg tggcaattct
     3661 accttaaatg tttttgcggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DAPBBG010000207         2974 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.139, whole genome shotgun sequence.
ACCESSION   DAPBBG010000207 DAPBBG010000000
VERSION     DAPBBG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.139"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            296..1264
                     /locus_tag="RD477_003046"
     CDS             296..1264
                     /locus_tag="RD477_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDT7635292.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1257..2288
                     /gene="vanA"
                     /locus_tag="RD477_003047"
     CDS             1257..2288
                     /gene="vanA"
                     /locus_tag="RD477_003047"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDT7635293.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2294..2902
                     /locus_tag="RD477_003048"
     CDS             2294..2902
                     /locus_tag="RD477_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDT7635294.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          885 a          568 c          719 g          802 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgc ttccagcgat gccagacttg
       61 gttgataaaa ggaggtccta agagatgtat ataatttttt aggaaaatct caaggttatc
      121 tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt cttacacggt
      181 agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt gacaagatta
      241 ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct taattatgaa
      301 taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat tccatgctct
      361 ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg aatccaacgc
      421 caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga tttccgcctc
      481 tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa gcatcggctg
      541 caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca atgtggcgta
      601 ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag tacgcaacgt
      661 aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg accgtggcaa
      721 ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca aagcggttat
      781 tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc gaagtataga
      841 ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta cgcttcatgt
      901 gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga gaatgaagca
      961 aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg agttggttaa
     1021 agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag gagaggaaga
     1081 gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac ttaaacttca
     1141 aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc aagcgttgcg
     1201 tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac aggagcatga
     1261 atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac gtatcggtaa
     1321 aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg ttatacattg
     1381 gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa tgggaaaacg
     1441 acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta cttgttaaaa
     1501 agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg catggcaagt
     1561 caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct tttgtaggct
     1621 gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc gttgcgaaaa
     1681 atgctgggat agctactccc gccttttggg ttattaataa agatgatagg ccggtggcag
     1741 ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc ttcggtgtga
     1801 aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga caatatgaca
     1861 gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa
     1921 acagtgccgc gttagttgtt ggcgaggtgg accaaatcag gctgcagtac ggaatctttc
     1981 gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata accgttcccg
     2041 cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa atatataaag
     2101 cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac ggccgcattg
     2161 tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat ccccgtatga
     2221 tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta ttagcgttaa
     2281 aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac acggtgttcg
     2341 ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg acggttatga
     2401 agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg caaaagaact
     2461 ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta agcgtgctgt
     2521 aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg aaagttatta
     2581 tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa aatcaagcca
     2641 tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg agcttgtacc
     2701 aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa atggaatatc
     2761 atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca gtgggtttga
     2821 agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc ccaatagcta
     2881 ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat ataagcggtt
     2941 ctgttgcaaa gttttaaata aagaataaaa tccc
//
LOCUS       DAPBBG010000208         2239 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.147, whole genome shotgun sequence.
ACCESSION   DAPBBG010000208 DAPBBG010000000
VERSION     DAPBBG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.147"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            216..1127
                     /locus_tag="RD477_003049"
     CDS             216..1127
                     /locus_tag="RD477_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDT7635295.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1280..1765
                     /gene="vanZ"
                     /locus_tag="RD477_003050"
     CDS             1280..1765
                     /gene="vanZ"
                     /locus_tag="RD477_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDT7635296.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2020..2239)
                     /locus_tag="RD477_003051"
                     /pseudo
     CDS             complement(2020..2239)
                     /locus_tag="RD477_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          786 a          318 c          424 g          711 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgt atcacttatg aaaattcatc
       61 tacattggtg ataatagtaa atccagtagg gcgaaataat tgactgtaat ttacggggca
      121 aaacggcaca atctcaaacg agattgtgcc gtttaagggg aagattctag aaatatttca
      181 tacttccaac tatatagtta aggaggagac tgaaaatgaa gaagttgttt tttttattgt
      241 tattgttatt cttaatatac ttaggttatg actacgttaa tgaagcactg ttttctcagg
      301 aaaaagtcga atttcaaaat tatgatcaaa atcccaaaga acatttagaa aatagtggga
      361 cttctgaaaa tacccaagag aaaacaatta cagaagaaca ggtttatcaa ggaaatctgc
      421 tattaatcaa tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat atcgtgaatt
      481 tatctaaaca tgacgaatta ataaatggat acgggttgct tgatagtaat atttatatgt
      541 caaaagaaat agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag ggtggcgtta
      601 gtcattttat tattaatagt ggctatcgag actttgatga gcaaagtgtg ctttaccaag
      661 aaatgggggc tgagtatgcc ttaccagcag gttatagtga gcataattca ggtttatcac
      721 tagatgtagg atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag tggatagaag
      781 aaaatgcttg gaaatacggg ttcattttac gttatccaga ggacaaaaca gagttaacag
      841 gaattcaata tgaaccatgg catattcgct atgttggttt accacatagt gcgattatga
      901 aagaaaagaa tttcgttctc gaggaatata tggattacct aaaagaagaa aaaaccattt
      961 ctgttagtgt aaatggggaa aaatatgaga tcttttatta tcctgttact aaaaatacca
     1021 ccattcatgt gccgactaat cttcgttatg agatatcagg aaacaatata gacggtgtaa
     1081 ttgtgacagt gtttcccgga tcaacacata ctaattcaag gaggtaagga tggcggaatg
     1141 aaaccaacga aattaatgaa cagcattatt gtactagcac ttttggggta acgttagctt
     1201 tttaatttaa aacccacgtt aactaggaca ttgctatact aatgatacaa cttaaacaaa
     1261 agaattagag gaaattatat tgggaaaaat attatctaga ggattgctag ctttatattt
     1321 agtgacacta atctggttag tgttattcaa attacaatac aatattttat cagtatttaa
     1381 ttatcatcaa agaagtctta acttgactcc atttactgct actgggaatt tcagagagat
     1441 gatagataat gttataatct ttattccatt tggcttgctt ttgaatgtca attttaaaga
     1501 aatcggattt ttacctaagt ttgcttttgt actggtttta agtcttactt ttgaaataat
     1561 tcaatttatc ttcgctattg gagcgacaga cataacagat gtaattacaa atactgttgg
     1621 aggctttctt ggactgaaat tatatggttt aagcaataag catatgaatc aaaaaaaatt
     1681 agacagagtt attatttttg taggtatact tttgctcgta ttattgctcg tttaccgtac
     1741 ccatttaaga ataaattacg tgtaagatgt ctaaatcaag caatctgatc tttcatacac
     1801 ataaagatat tgaatgaatt ggattagatg gaaaacggga tgtggggaaa ctcgcccgta
     1861 ggtgtgaagt gaggggaaaa ccggtgataa agtaaaaagc ttacctaaca ctatagtaac
     1921 aaagaaagcc caattatcaa ttttagtgct gaggaattgg tctctttaat aaatttcctt
     1981 aacgttgtaa atccgcattt tcctgacggt acccccaaac taagatttca tagatagatt
     2041 ttctgataac tttaaaaaac acgaatagcg gaaaggatgt cttcattcgc taaagagtaa
     2101 taaactactt ttccatcttg acgtgatttt gccattccat gtttttttaa aaaacgtaaa
     2161 tggtgtgacg ttgtagcgac actagcagta agtatttctg cggttctgtt gcaaagtttt
     2221 aaatctacta tcaaataag
//
LOCUS       DAPBBG010000209         2235 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.148, whole genome shotgun sequence.
ACCESSION   DAPBBG010000209 DAPBBG010000000
VERSION     DAPBBG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.148"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(724..1014)
                     /locus_tag="RD477_003052"
     CDS             complement(724..1014)
                     /locus_tag="RD477_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7635297.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1222..1914)
                     /locus_tag="RD477_003053"
     CDS             complement(1222..1914)
                     /locus_tag="RD477_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDT7635298.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          643 a          410 c          323 g          859 t
ORIGIN      
        1 tatggatttt ttcatttaca caaaatattt tatactctcg agtataacat agctttaagc
       61 attacttaaa gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt
      121 gatgcaaaag aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt
      181 tcttagttaa ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga
      241 catacaatca tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa
      301 tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt
      361 caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg
      421 aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc tctttttatg
      481 gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt
      541 taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata
      601 catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc
      661 atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt
      721 tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata
      781 ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct
      841 attattataa taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt
      901 caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct
      961 ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt
     1021 cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc
     1081 tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga
     1141 aggacatgta ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt
     1201 aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt
     1261 atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa
     1321 gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc
     1381 tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt
     1441 ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag
     1501 atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg
     1561 tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc
     1621 tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc
     1681 tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat
     1741 cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc
     1801 aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc
     1861 aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat
     1921 agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag
     1981 cgtttttaaa ttacatatgt aattttaata taaatactat caaatactca tttaatcctc
     2041 cttttttatc tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta
     2101 aataaatatt tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc
     2161 atttcataga ttactgtatt tattacaaaa aacgcggatt tgtcataaat tgtcatgctt
     2221 cttttaccta tgaaa
//
LOCUS       DAPBBG010000210         1412 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.163, whole genome shotgun sequence.
ACCESSION   DAPBBG010000210 DAPBBG010000000
VERSION     DAPBBG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.163"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1295
                     /locus_tag="RD477_003054"
     CDS             <1..1295
                     /locus_tag="RD477_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDT7635299.1"
                     /translation="YTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDE
                     IVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKS
                     GFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIG
                     SGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISD
                     ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNK
                     QNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLD
                     GNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQ
                     DIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          600 a          109 c          241 g          462 t
ORIGIN      
        1 attatacaga gccttgggaa gatgaagttt ttagagtaat tattgaatat aacaatgttc
       61 ctattggata tggacaaata tataaaatgt atgatgagtt atatactgat tatcattatc
      121 caaaaactga tgagatagtc tatggtatgg atcaatttat aggagagcca aattattgga
      181 gtaaaggaat tggtacaaga tatattaaat tgatttttga atttttgaaa aaagaaagaa
      241 atgctaatgc agttatttta gaccctcata aaaataatcc aagagcaata agggcatacc
      301 aaaaatctgg ttttagaatt attgaagatt tgccagaaca tgaattacac gagggcaaaa
      361 aagaagattg ttatttaatg gaatatagat atgatgataa tgccacaaat gttaaggcaa
      421 tgaaatattt aattgagcat tactttgata atttcaaagt agatagtatt gaaataatcg
      481 gtagtggtta tgatagtgtg gcatatttag ttaataatga atacattttt aaaacaaaat
      541 ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc aatatataat tttttaaata
      601 caaatttaga aactaatgta aaaattccta atattgaata ttcgtatatt agtgatgaat
      661 tatctatact aggttataaa gaaattaaag gaactttttt aacaccagaa atttattcta
      721 ctatgtcaga agaagaacaa aatttgttaa aacgagatat tgccagtttt ttaagacaaa
      781 tgcacggttt agattataca gatattagtg aatgtactat tgataataaa caaaatgtat
      841 tagaagagta tatattgttg cgtgaaacta tttataatga tttaactgat atagaaaaag
      901 attatataga aagttttatg gaaagactaa atgcaacaac agtttttgag ggtaaaaagt
      961 gtttatgcca taatgatttt agttgtaatc atctattgtt agatggcaat aatagattaa
     1021 ctggaataat tgattttgga gattctggaa ttatagatga atattgtgat tttatatact
     1081 tacttgaaga tagtgaagaa gaaataggaa caaattttgg agaagatata ttaagaatgt
     1141 atggaaatat agatattgag aaagcaaaag aatatcaaga tatagttgaa gaatattatc
     1201 ctattgaaac tattgtttat ggaattaaaa atattaaaca ggaatttatc gaaaatggta
     1261 gaaaagaaat ttataaaagg acttataaag attgattata taatatatga aaagctatta
     1321 taaaagacat tagtattaaa tagtttaaaa aaatgaaaaa taataaagga agtgagtcaa
     1381 gtccagactc ctgtgtaaaa tgctatacaa tg
//
LOCUS       DAPBBG010000211         1123 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-002
            SAMN36683392-rid18909803.guided.170, whole genome shotgun sequence.
ACCESSION   DAPBBG010000211 DAPBBG010000000
VERSION     DAPBBG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683392
            Sequence Read Archive: SRR25390910
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831679.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831679.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 22:18:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,056
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 41 of 89
            Pseudo Genes (incomplete)         :: 32 of 89
            Pseudo Genes (internal stop)      :: 27 of 89
            Pseudo Genes (multiple problems)  :: 9 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683392-rid18909803.guided.170"
                     /isolate="VREC-002"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            24..416
                     /locus_tag="RD477_003055"
     CDS             24..416
                     /locus_tag="RD477_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7635300.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1123
                     /gene="aac(6')-Ie"
                     /locus_tag="RD477_003056"
     CDS             417..>1123
                     /gene="aac(6')-Ie"
                     /locus_tag="RD477_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HDT7635301.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKYYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKG"
BASE COUNT          455 a           87 c          196 g          385 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatgg tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa tattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa agg
//
